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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

HA-LPA4-res/pCAGEN (#RDB17290)

Expression vector of mose LPA4.

Clone info. Expression vector of mose lysophosphatidic acid receptor 4 (LPA4) tagged with HA at N-terminus, CAG promotor. The LPA4 ORF containds silent mutation.
Comment Expression was confirmed by the depositor with immunocytochemistry and western blotting.
Vector backbone pCAGEN (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name mouse LPA4 cDNA
Depositor|Developer Kurabayashi, Nobuhiro |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kurabayashi N. et al. Development, 145(17), pii: dev162529, 2018.)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kurabayashi N. et al. Development, 145(17), pii: dev162529, 2018.)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17290 HA-LPA4-res/pCAGEN DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-LPA4-res/pCAGEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17290).

Reference section:

Kurabayashi, N., Tanaka, A., Nguyen, M.D., Sanada, K., The LPA-LPA4 axis is required for establishment of bipolar morphology and radial migration of newborn cortical neurons. Development 145 (17). pii: dev162529 (2018). PMID 30217809. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17290_A9Dbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: HA, insert 5'
Sequence file: RDB17290_A9Dba.seq check
>D06232A2_A9Db_1_pAxCA_F1_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN NTGCCTTCTT CTTTTTCCTA CNGCTCCTGG GCAACGTGCT
   61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GAGGATATGT ACCCATACGA
  121 TGTTCCAGAT TACGCTATGG GTGACAGAAG ATTTATTGAC TTCCAATTCC AAGATTTAAA
  181 TTCAAGTCTC AGACCCAGGT TGGGAAATGC AACTGCCAAT AATACTTGCA TTGTTGATGA
  241 TTCCTTCAAG TATAATTTGA ATGGTGCTGT CTATAGTGTT GTATTCATCC TGGGTCTAAT
  301 AACCAACAGT GCCTCCCTGT TTGTCTTCTG CTTCCGCATG AAAATGAGAA GTGAGACGGC
  361 TATTTTCATC ACCAACCTGG CCCTCTCTGA TTTGCTTTTT GTTTGTACCC TACCTTTCAA
  421 AATATTTTAC AACTTTAATC GCCACTGGCC TTTTGGTGAC ACCCTCTGTA AGATCTCAGG
  481 GACTGCGTTC CTCACCAACA TCTATGGGAG CATGCTGTTT CTCACCTGCA TCAGTGTGGA
  541 TCGTTTCCTA GCCATTGTCT ATCCCTTCCG ATCGCGTACC ATCAGGACCA GGAGGAATTC
  601 CGCCATTGTG TGCGCTGGAG TCTGGATCCT AGTCCTCAGT GGTGGTATTT CAGCTTCTTT
  661 GTTCTCCACC ACTAATGTCA ACAATGCGAC CACCACTTGC TTTGAAGGCT TCTCCAAACG
  721 TGTCTGGAAG ACATACCTGT CNNAGATCAC TATATTCATT GAAGTTGTTG GATTCATCAT
  781 TCCTCTGATA TTGAATGTTT CTTGTTCTTC TGTGGTGCTT ANAACCCTCC GCAAGCCTGC
  841 AACATTGTCT CAGANTGGGN NNNATAAAAA AAAAAGTGNT GAAGATGATN ACAGTGCATA
  901 TGGCAGTGTT TGTGGTATGC TTTGNACCNT ACNNCTCCNT TCTCTTTTTA TATGCCNTNN
  961 NANNCTNNNN NNCNNTTACT AANTNGCTTA NTNNNAANNN NTNGNNNNNT CNTGNANCNN
1021 ANTNNNNNNN NNN
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globin pA, insert 3'
Sequence file: RDB17290_A9Dbb.seq check
>D06232A2_A9Db_1_pCAGGS_R1_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCN NTTNNNNNNA AANTAAATTT CCTTTATTAG CCAGAAGTCA
   61 GATGCTCAAG GGGCTTCATG ATGTCCCCAT AATTTTTGGC AGAGGGAAAA AGATCTCAGT
  121 GGTATTTGTG AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA
  181 CCTGAGGAGT GCGGCCGCGA TCTAGAAGGT GGATTCCAGC ATTAATTCAC CACCATTATT
  241 TGTTGTTTGA TCACTAACTT CCTCTTGGAT AGCTGGAAGG GAAGGTTTGG GGGTCAGAGG
  301 TGTCTCAGTC TTAAACAGCG ACTCCATCCT TATATGTGTA TTGATATAAA AGGACTTCTG
  361 AAAGGATTCA AGAGTGAAGT AATAGATAAA AGGATCAAAG CAACAATTCA GAGTTGCAAG
  421 GCACAAGGTA ATTGGGTACA TGATCTTTGC AAACCTTTCC AATAAGCAAT TAGTAATGGC
  481 TTGGGAGCGT ACCAAGGCAT ATAAAAAGAG AACGGAGTTG TATGGTACAA AGCATACCAC
  541 AAACACTGCC ATATGCACTG TGATCATCTT CAACACTTTT TTTTTATTGG TCCCAATCTG
  601 AGACAATGTT GCAGGCTTGC GGAGGGTTCT AAGCACCACA GAAGAACAAG AAACATTCAA
  661 TATCAGAGGA ATGATGAATC CAACAACTTC AATGAATATA GTGATCTTGG ACAGGTATGT
  721 CTTCCAGACA CGTTTGGAGA AGCCTTCAAA GCAAGTGGTG GTCGCATTGT TGACATTAGT
  781 GGTGGAGAAC AAAGAAGCTG AAATACCACC ACTGNNGANT ANGATCCAGA CTCCAGCGCA
  841 CACAATGGCG GANTTCCTCC NGGTNCTGAT GGTACNCGAT CGGNANNGNA TAGACAATGG
  901 CTANGAAANG ATCCNCACTG ATGNNNNGAN AAACAGCANG CNNCCNTNGA TGNNNNNNAN
961 NNNNNNANNC CNNNANNTNN NACANANGGN NNNNNNNAAA NNNCANNNNN NNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori, SV40 pA, ori
Sequence file: RDB17290_A9Dbc.seq check
>D06232A2_A9Db_1_SV40pro_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA NNNGTGAGGA NGNTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT
  121 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
  181 TATCTTATCA TGTCTGGATC CGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG
  241 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
  301 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
  361 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
  421 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
  481 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
  541 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
  601 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
  661 GGTGTAGGTC GTTCGCTCCN AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
  721 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
  781 ACTGGCAGCA GCCACTGGTA ACAGGNTTAG CAGANCGANG TATGTANGCG GTGCTACAGA
  841 GTTCTTGAAG TGGTGGNCNA ACTACGGCTA CNNTANAANA ACNGTATTTG GTATCTGCNC
  901 TCTGCTGAAG CNNGTTACCT TNNGAAAAAN ANTTGGTAGC TCNTNNTNCG GNNANNAAAN
  961 NNNNGCNGGN ANCGGNNNNT TTTTTTNNNN NNNNCNNCNN ANNNNNCNNN NNAAAAAANN
1021 NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kurabayashi, N., The LPA-LPA4 axis is required for establishment of bipolar morphology and radial migration of newborn cortical neurons. Development 145 (17). pii: dev162529 (2018). PMID 30217809. [link to RRC of NBRP]