Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFPN3-mMAP7D1

Expression vector of mouse Map7d1 fused with EGFP.

Catalog number RDB16945
Resource name pEGFPN3-mMAP7D1
Clone info. Expression vector of mouse microtubule associated protein 7 domain containing 1 (Map7d1) fused with EGFP at C-terminus.
Comment Expression was confirmed by the depositor with western blotting, immunofluorescence, immunoprecipitates, live imaging.
Vector backbone pEGFP-N3 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kam^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Map7d1 cDNA
Depositor|Developer Kikuchi, Koji |
Other clones in our bank

External Database
mouse Map7d1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (EMBO Rep. 19 (7), pii: e45471, 2018.)
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 (EMBO Rep. 19 (7), pii: e45471, 2018.)
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16945 pEGFPN3-mMAP7D1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kikuchi, K., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0100e (English text)
          Featured content Dnaconda's recommendation EXR0100j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16945_A9D8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB16945_A9D8a.seq check
          >D06215A3-A9D8_1_CMV_Forward_F02_17_ABI24.ab1
              1 ACGGTAAACC TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGAC TCAGATCTCG
            121 AGCTCAAGCT TCGAATTCCC GGGATATCGT CGACCCACGC GTCCGGCAGA ATCGCGGTCG
            181 CGGAGGACCG ACCTCGGGTA GCTGGCTACT CAGAGCTTCG TTAGCCACCG GCCTCTACCT
            241 CTCCCACTCC GAACGCCGCC GCCTCTGGGT CTTTGCCCGC TGGGCGGGCC GGGCGGGATG
            301 CGCCGCGGGA CCCTGGTCCT GGCCTCCGGC CGTCGCCGCC GTCGCGGCTG AGACCCTCCC
            361 CCAAAACCTC CAGTGACGCC GCAGCCATGG AGAGTGGCCC GCGTGTGGAG CCCGGCCCGG
            421 GCGCACCCGC AGCTGTGTTA GCCAGGATCC CCCAAGAGCC AAGACCGTCT CCGGAAGGTG
            481 ACCCTTCCCC TCCTCCGCCG CCAACACCGA TGTCAGCCTT GGTTCCTGAC ACTCCCCCAG
            541 ACACACCTCC TGCCCTGAAG ACTGCCACGA ATCCTAAGCA GCTCCCACTG GAACCAGGGA
            601 ATCCCACAGG GCAGATCTCA CCTCAGCCAG CTCCCCCACA GGAAGAATGC CCGTCCTCAG
            661 AAGCCAAGAG CAGGGGCCCC ACCCCCACAG CCACAGGACC ACGGGAGGCC AAGCCTTCTC
            721 GAAGAAGCAG CCAACCATCC CCCACCACAG TGTCAGCCTA TGACAGCCCT CCTGCCAAGC
            781 AAGATGTAAA GAAGGCAGGA GAGAGACACA AGCTTGCCAA AGAGCGCCGA GAAGAGCGGG
            841 CCAAGTACCT GGCGGCCAAG AAAGGCAGTG TGGTTGGAGA AGGAGGAAAA AGCCCAAGGC
            901 GCTCCGGGAA AAGCAACTCC AGGAGCGCCG CCGACGGCTG GAGGAGCAGC GGCTTAAAGC
            961 CGAACAGCGC CGGGCTGCCC TGAGGAGCGG CAGAGCAGAA GCTTGAGAAA AACAGGAGCG
           1021 CTATGAAGCA GCAATCCAGC GGTCAGTGAG AGACGTGGGC TGAGATTCGG CCAACAGCGC
           1081 TGGTCTGGCA GGGTCTGCAC ACAGCTCCCC TGCACGTAGA CAGTGGGAAG CCAAGGGTGC
          1141 CTCTCCG
          //
          Primer: EGFP-N
          Sequence file: RDB16945_A9D8b.seq check
          >D06215A3_A9D8_1_EGFP-N_A02_02_ABI24.ab1
              1 GCCAACCCGG GAGACAGCTC CTCGCCCTTG CTCACCATGG TGGCGATGGA TCCCGGGCCC
             61 GCGGTACCGT CGACAAGAAC TTCTGTGACC TGTGGAGGCT GCACCACTGC TTTCTTGAGG
            121 AAGGCTTCTG CCTCTGCAAA TGGGAGGAGC CCCTCTGCTG TGCGACCCAG ACTCTTGTCC
            181 CCAGCGGTTC CCTTTGGGGA GAAGCCGTTC TCCTGGTGCG CTGGGAGAGG CTGCAGGCCA
            241 TTTACCAGGG ACCCTGGCAT CTCCTTGCTT GGCAGGCTCC ACTGTGGCTC CTGGGGGGCC
            301 AGCTCCTCTT TCTGCATAGA ATCCTTCTGT AGCCCAGAGG GCCGAGTCTC TACAGCTTTC
            361 ACAGGGTCTG GGCCGGAATT GTTAGCTGCG GTCTCCTTTG CATCCTGCTT CTTGGTTTCG
            421 GCGGCTTCTG ATTTCCGAGT CCTCTTCATT ATCTCCTCCA GCCGCTTTCT TCGCTCTTGT
            481 CTCTCCTGTT CCTCCTTCTG AAAGTGCTTT TCCCGCTCCT GGCGCTGGCG CTCAGCTTCT
            541 TCCCGGGACC GGGCTTCGGC TTCCTCTTTC TGCTTCTGCA GTCGCTCCTG CTCCTCCTGC
            601 TCAGCCTGCG CCTTCTCTCG AGCCTCCTGC TCCTCCCGTC TCCGCGCCTC GGCCTCACGT
            661 TCAGCCCGGG CTTCAGCCTC CCGGGCCAGC TGCTCCTCTC GCATTCGCTT GTCCCTTTCA
            721 GCCTGCAGCT TCCGCTCCTG TTCTTCGCGT TCCCTCTGTT CCCGAGCCTG GCGCCGCTTT
            781 TCAGCCAGGA GCCGCGTGGC TTCCTCTCGG TCTGTGGTGC CCGCCATGGG TTTGCTAGGG
            841 GTCACCGAGG GAGCAGCGGT AGGAGGGGCA GTCGGAGCAG CTGGGACAGC CGGGACAGCA
            901 GTTTCTGCAG GGATCTGTGT TGAGGATTGC TCCTTCTGAG GCTGGGCTGG GGTGGGCGAG
            961 GGCACGGTGA GGGCGCTGGT GAAGGCTGGG GCAGCTGCTC CTTCTCCTCA GCGGCTCTAC
           1021 TCTGGCGTGA TTTTGTCCTC AAGCCCGACG GGCTGGGGCT CTCTTGCTCT ATCCTTCGAC
           1081 CGCTCCTGGT GATGCATGGT TGCTCGGGAT GTGATTGGAG AAGCAATTCC TGATCTCCTG
          1141 GGCTCTGTGG GGCACCAG
          //
          Primer: ME-1290RV
          Sequence file: RDB16945_A9D8c.seq check
          >D06215A3_A9D8_1_ME-1290RV_B02_05_ABI24.ab1
              1 AGTTCAATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
             61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTTACTTG
            121 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
            181 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGGTGCTCAG GTAGTGGTTG
            241 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
            301 TGCACGCTGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TCACCTTGAT GCCGTTCTTC
            361 TGCTTGTCGG CCATGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC
            421 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG
            481 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG
            541 GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG
            601 TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC GAGGGTGGGC
            661 CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTAGGTG
            721 GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC
            781 TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG
            841 ATGGATCCCG GGCCCGCGGT ACCGTCGACA AGAACTTCTG TGACCTGTGG AGGCTGCACC
            901 ACTGCTTTCT TGAGGAAGGC TTCTGCCTCT GCAAATGGGA GGAGCCCCTC TGCTGTGCGA
            961 CCCAGACTCT TGTCCCCAGC GGGTTCCCTT TGGGGAGAAG CCGTTCTCCT GGTGCGCTGG
           1021 GAGAGGCTGC AGGCCCATTT ACCAGGGAAC CCTGACATCT CCTTTGCTTG GGCAGCTCAA
           1081 CTGTGCTCTT GGGGGCCAGC TCCTCTTCTG CCATAGATTC TTCTGTAGCC AGACGCCGAT
          1141 CCTACAGCCT TCACAGGCTC TCTGA
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB16945_A9D8d.seq check
          >D06215A3-A9D8_1_SV40pro_F_V2_H02_23_ABI24.ab1
              1 CACCAACTTT TTTTGGAGGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
             61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
            601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
            901 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
            961 CGGAATCGTT TTCCGGGACG CCGGGCTGGA TGATCCTCCA GCGCGGGGGA TCTCATGCTG
           1021 GGAGTTCTTC GCCCACCCCT AGGGGGAGCT ACTGAAACAC GGGAGGAGAC AATACCGTAG
           1081 AACCCGCGCT ATGACGGCCA TAAAAGACGA TAACGCACGG TGTTGGGTCG TTTGGTTTCC
          1141 AATTAAAAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kikuchi, K., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl