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pEGFPN3-mMAP7D1 (#RDB16945)

Expression vector of mouse Map7d1 fused with EGFP.

Clone info. Expression vector of mouse microtubule associated protein 7 domain containing 1 (Map7d1) fused with EGFP at C-terminus.
Comment Expression was confirmed by the depositor with western blotting, immunofluorescence, immunoprecipitates, live imaging.
Vector backbone pEGFP-N3 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kam^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Map7d1 cDNA
Depositor|Developer Kikuchi, Koji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (EMBO Rep. 19 (7), pii: e45471, 2018.)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 (EMBO Rep. 19 (7), pii: e45471, 2018.)

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RDB16945 pEGFPN3-mMAP7D1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFPN3-mMAP7D1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16945).

Reference section:

Kikuchi, K., Nakamura, A., Arata, M., Shi, D., Nakagawa, M., Tanaka, T., Uemura, T., Fujimori, T., Kikuchi, A., Uezu, A., Sakamoto, Y., Nakanishi, H., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16945_A9D8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB16945_A9D8a.seq check
>D06215A3-A9D8_1_CMV_Forward_F02_17_ABI24.ab1
    1 ACGGTAAACC TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGAC TCAGATCTCG
  121 AGCTCAAGCT TCGAATTCCC GGGATATCGT CGACCCACGC GTCCGGCAGA ATCGCGGTCG
  181 CGGAGGACCG ACCTCGGGTA GCTGGCTACT CAGAGCTTCG TTAGCCACCG GCCTCTACCT
  241 CTCCCACTCC GAACGCCGCC GCCTCTGGGT CTTTGCCCGC TGGGCGGGCC GGGCGGGATG
  301 CGCCGCGGGA CCCTGGTCCT GGCCTCCGGC CGTCGCCGCC GTCGCGGCTG AGACCCTCCC
  361 CCAAAACCTC CAGTGACGCC GCAGCCATGG AGAGTGGCCC GCGTGTGGAG CCCGGCCCGG
  421 GCGCACCCGC AGCTGTGTTA GCCAGGATCC CCCAAGAGCC AAGACCGTCT CCGGAAGGTG
  481 ACCCTTCCCC TCCTCCGCCG CCAACACCGA TGTCAGCCTT GGTTCCTGAC ACTCCCCCAG
  541 ACACACCTCC TGCCCTGAAG ACTGCCACGA ATCCTAAGCA GCTCCCACTG GAACCAGGGA
  601 ATCCCACAGG GCAGATCTCA CCTCAGCCAG CTCCCCCACA GGAAGAATGC CCGTCCTCAG
  661 AAGCCAAGAG CAGGGGCCCC ACCCCCACAG CCACAGGACC ACGGGAGGCC AAGCCTTCTC
  721 GAAGAAGCAG CCAACCATCC CCCACCACAG TGTCAGCCTA TGACAGCCCT CCTGCCAAGC
  781 AAGATGTAAA GAAGGCAGGA GAGAGACACA AGCTTGCCAA AGAGCGCCGA GAAGAGCGGG
  841 CCAAGTACCT GGCGGCCAAG AAAGGCAGTG TGGTTGGAGA AGGAGGAAAA AGCCCAAGGC
  901 GCTCCGGGAA AAGCAACTCC AGGAGCGCCG CCGACGGCTG GAGGAGCAGC GGCTTAAAGC
  961 CGAACAGCGC CGGGCTGCCC TGAGGAGCGG CAGAGCAGAA GCTTGAGAAA AACAGGAGCG
 1021 CTATGAAGCA GCAATCCAGC GGTCAGTGAG AGACGTGGGC TGAGATTCGG CCAACAGCGC
 1081 TGGTCTGGCA GGGTCTGCAC ACAGCTCCCC TGCACGTAGA CAGTGGGAAG CCAAGGGTGC
1141 CTCTCCG
//
Primer: EGFP-N ()
Sequence file: RDB16945_A9D8b.seq check
>D06215A3_A9D8_1_EGFP-N_A02_02_ABI24.ab1
    1 GCCAACCCGG GAGACAGCTC CTCGCCCTTG CTCACCATGG TGGCGATGGA TCCCGGGCCC
   61 GCGGTACCGT CGACAAGAAC TTCTGTGACC TGTGGAGGCT GCACCACTGC TTTCTTGAGG
  121 AAGGCTTCTG CCTCTGCAAA TGGGAGGAGC CCCTCTGCTG TGCGACCCAG ACTCTTGTCC
  181 CCAGCGGTTC CCTTTGGGGA GAAGCCGTTC TCCTGGTGCG CTGGGAGAGG CTGCAGGCCA
  241 TTTACCAGGG ACCCTGGCAT CTCCTTGCTT GGCAGGCTCC ACTGTGGCTC CTGGGGGGCC
  301 AGCTCCTCTT TCTGCATAGA ATCCTTCTGT AGCCCAGAGG GCCGAGTCTC TACAGCTTTC
  361 ACAGGGTCTG GGCCGGAATT GTTAGCTGCG GTCTCCTTTG CATCCTGCTT CTTGGTTTCG
  421 GCGGCTTCTG ATTTCCGAGT CCTCTTCATT ATCTCCTCCA GCCGCTTTCT TCGCTCTTGT
  481 CTCTCCTGTT CCTCCTTCTG AAAGTGCTTT TCCCGCTCCT GGCGCTGGCG CTCAGCTTCT
  541 TCCCGGGACC GGGCTTCGGC TTCCTCTTTC TGCTTCTGCA GTCGCTCCTG CTCCTCCTGC
  601 TCAGCCTGCG CCTTCTCTCG AGCCTCCTGC TCCTCCCGTC TCCGCGCCTC GGCCTCACGT
  661 TCAGCCCGGG CTTCAGCCTC CCGGGCCAGC TGCTCCTCTC GCATTCGCTT GTCCCTTTCA
  721 GCCTGCAGCT TCCGCTCCTG TTCTTCGCGT TCCCTCTGTT CCCGAGCCTG GCGCCGCTTT
  781 TCAGCCAGGA GCCGCGTGGC TTCCTCTCGG TCTGTGGTGC CCGCCATGGG TTTGCTAGGG
  841 GTCACCGAGG GAGCAGCGGT AGGAGGGGCA GTCGGAGCAG CTGGGACAGC CGGGACAGCA
  901 GTTTCTGCAG GGATCTGTGT TGAGGATTGC TCCTTCTGAG GCTGGGCTGG GGTGGGCGAG
  961 GGCACGGTGA GGGCGCTGGT GAAGGCTGGG GCAGCTGCTC CTTCTCCTCA GCGGCTCTAC
 1021 TCTGGCGTGA TTTTGTCCTC AAGCCCGACG GGCTGGGGCT CTCTTGCTCT ATCCTTCGAC
 1081 CGCTCCTGGT GATGCATGGT TGCTCGGGAT GTGATTGGAG AAGCAATTCC TGATCTCCTG
1141 GGCTCTGTGG GGCACCAG
//
Primer: ME-1290RV ()
Sequence file: RDB16945_A9D8c.seq check
>D06215A3_A9D8_1_ME-1290RV_B02_05_ABI24.ab1
    1 AGTTCAATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
   61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTTACTTG
  121 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
  181 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGGTGCTCAG GTAGTGGTTG
  241 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
  301 TGCACGCTGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TCACCTTGAT GCCGTTCTTC
  361 TGCTTGTCGG CCATGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC
  421 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG
  481 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG
  541 GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG
  601 TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC GAGGGTGGGC
  661 CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTAGGTG
  721 GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC
  781 TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG
  841 ATGGATCCCG GGCCCGCGGT ACCGTCGACA AGAACTTCTG TGACCTGTGG AGGCTGCACC
  901 ACTGCTTTCT TGAGGAAGGC TTCTGCCTCT GCAAATGGGA GGAGCCCCTC TGCTGTGCGA
  961 CCCAGACTCT TGTCCCCAGC GGGTTCCCTT TGGGGAGAAG CCGTTCTCCT GGTGCGCTGG
 1021 GAGAGGCTGC AGGCCCATTT ACCAGGGAAC CCTGACATCT CCTTTGCTTG GGCAGCTCAA
 1081 CTGTGCTCTT GGGGGCCAGC TCCTCTTCTG CCATAGATTC TTCTGTAGCC AGACGCCGAT
1141 CCTACAGCCT TCACAGGCTC TCTGA
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB16945_A9D8d.seq check
>D06215A3-A9D8_1_SV40pro_F_V2_H02_23_ABI24.ab1
    1 CACCAACTTT TTTTGGAGGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
  901 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  961 CGGAATCGTT TTCCGGGACG CCGGGCTGGA TGATCCTCCA GCGCGGGGGA TCTCATGCTG
 1021 GGAGTTCTTC GCCCACCCCT AGGGGGAGCT ACTGAAACAC GGGAGGAGAC AATACCGTAG
 1081 AACCCGCGCT ATGACGGCCA TAAAAGACGA TAACGCACGG TGTTGGGTCG TTTGGTTTCC
1141 AATTAAAAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kikuchi, K., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710. [link to RRC of NBRP]