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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1-V5His6-hMap7

Expression vector of human microtubule associated protein 7 (MAP7) tagged with V5 and 6xHis.

Catalog number RDB16944
Resource name pcDNA3.1-V5His6-hMap7
Clone info. Expression vector of human microtubule associated protein 7 (MAP7) tagged with V5 and 6xHis at C-terminus.
Comment Expression was confirmed by the depositor with western blotting, immunofluorescence, immunoprecipitates. The cDNA encoding hMAP7 was provided by the RIKEN BRC (IRAK049L15, Cat No. HGX019879).
Vector backbone pcDNA3.1-V5His6 (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human MAP7 cDNA
Depositor|Developer Kikuchi, Koji |
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External Database
human MAP7

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (EMBO Rep. 19 (7), pii: e45471, 2018.)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 (EMBO Rep. 19 (7), pii: e45471, 2018.)
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB16944 pcDNA3.1-V5His6-hMap7 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kikuchi, K., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0100e (English text)
          Featured content Dnaconda's recommendation EXR0100j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16944_A9D8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB16944_A9D8a.seq check
          >D06215A2_A9D8_2_CMV_Forward_E01_13_ABI24.ab1
              1 CTGGTTAAAC TTCCGCCCCC ATTTGGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG
             61 TCTATATAAG CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA
            121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCGAGCTCGG
            181 ATCCATGGCG GAGCTAGGAG CTGGCGGCGA CGGCCACAGG GGCGGCGACG GCGCAGTGCG
            241 AAGCGAAACA GCACCCGACA GCTACAAAGT GCAAGATAAG AAAAATGCCT CCAGCCGCCC
            301 TGCCTCTGCA ATTTCAGGAC AAAATAACAA CCACTCAGGA AATAAACCAG ACCCTCCGCC
            361 TGTGTTACGT GTTGATGACC GGCAGCGGCT GGCCCGGGAG CGACGTGAGG AACGGGAGAA
            421 ACAGCTAGCT GCAAGAGAAA TAGTGTGGTT AGAAAGAGAA GAGCGAGCCA GGCAGCACTA
            481 CGAGAAGCAC CTGGAAGAGC GGAAGAAGAG GTTGGAGGAG CAGAGGCAGA AGGAGGAGCG
            541 GAGGAGGGCT GCTGTGGAGG AGAAGCGGAG GCAGAGACTT GAGGAGGACA AAGAACGCCA
            601 CGAAGCTGTT GTACGGCGCA CAATGGAAAG GAGCCAGAAG CCAAAACAGA AGCATAACCG
            661 TTGGTCGTGG GGAGGCTCTC TCCATGGGAG CCCTAGCATC CACAGTGCAG ATCCAGACAA
            721 GGCGGTCAGT TTCCACCATG AATCTTTCGA AAATATGTTG ATCCCGTCAT TAGCAAGCGG
            781 CTCTCCTCTT CATCTGCAAC TTTACTAAAT TCTCCAGATA GAAGCTCGCC GCCTGCAGCT
            841 CAGCCCATGG GGAGAGGCAG CGTTTGTTAA CAGACTCCTG ACGCCCACAC ATTCGTTTCC
            901 TGGGCCAGAA GTAAAAGCAC AGCTGCCTTG TCTGGGAGAA GCAGCATCTT TGCAGCCCCC
            961 ATCATCATGC CCCTACAAAG CTGCACACTT CTAGAAATTC GAATGGGATC GACCAAAACT
           1021 CTTTTGTTAA CACCACCCTG AAGGGCTCTT TCTTCGCCAA GGAGGGATCA ATTCATGGGC
           1081 ACAGCGAAGC TTATAAAAAA GAAAGAGGAG AGAGGAAATG TTACTCTCTT TCCTCTCACA
           1141 TCCTGGCACC CCGAAAGGGG CTGTATTCCT CATTCTAAAT TCCCAAGTCA GACACCGAGC
          1201 TTCGGCCGTT GTC
          //
          Primer: f1ori_R3
          Sequence file: RDB16944_A9D8b.seq check
          >D06215A2_A9D8_2_f1ori_R3_F01_16_ABI24.ab1
              1 GGGGGGGAAA CTGGAGCCCA GCTGGTTCTT TCCGCCTCAG AAGCCATAGA GCCCACCGCA
             61 TCCCCAGCAT GCCTGCTATT GTCTTCCCAA TCCTCCCCCT TGCTGTCCTG CCCCACCCCA
            121 CCCCCCAGAA TAGAATGACA CCTACTCAGA CAATGCGATG CAATTTCCTC ATTTTATTAG
            181 GAAAGGACAG TGGGAGTGGC ACCTTCCAGG GTCAAGGAAG GCACGGGGGA GGGGCAAACA
            241 ACAGATGGCT GGCAACTAGA AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACTCAATGG
            301 TGATGGTGAT GATGACCGGT ACGCGTAGAA TCGAGACCGA GGAGAGGGTT AGGGATAGGC
            361 TTACCTTCGA ACCGCGGGCC CTCTAGACTC GAGCCTATAA CTTCTGCAGT CTGCTGTGTC
            421 TGAACACCAT CTACCTGAGG CAGGGGCCCA AGTGTCCCTT CATCATCAAA GGCCAAAATT
            481 GGATTCAAAG GAATTTCTGG GCTCTCACTG TTGGTGACAT CTAATCTGGA TGGTTTAGAT
            541 CCAATGGGTA AGTTTATAAT TTCTTCAAAA TTTTCATTCT GAACAGATAC ACCATTTTCA
            601 TTTGGTTGTT TTTCCAAATC GGGAGTGCTC TCCACTGTCA CTTTTGACTG GTGTGAGGTA
            661 ACCACATGTG GGCTACCAAC TGGCTTTCCA TTTCCCGGAG CGTTTGTTGT ACATGGAAGT
            721 GCAGACACCT CTGTTCCTCC AGTGAGAGCT CCCTTGGCTA TATCACCGTT TCTCTGATCA
            781 CTGGTTTTCT TATCTGTAGC TTCTGTTCTC CTGGTTCTTT TCATAATCTC CTCAAGTCGC
            841 TTCTTTCTCT CCAGGCGCTC TTGCTCTTCT CTCTGGAAAT GCTTCTCTCG TTCCTGCCGG
            901 ACCCTCTCTG CTTCTTCACG AACGCGAGCT TCTTCTTCTT TCTGCCTCTG GGCGCGCTCT
            961 GCCTCCTCCC ACTCGCGCAG CGCCCGCTCC TCCGCCTGCC GCTGCAGCTG CTCCCTCCTT
           1021 TCTCCCGGCC TGCTCGGGCT TTCCAGCCTG CGCGACTCTC CTCACGGCGA GTCGGTCCTC
           1081 TCTTCAGCCA CACGTGGAGC CCACTCGTCT CTCATTTGGT CATTCCAGGC TCATTCTTGC
          1141 CTTCCCGTCC CTCCTTCCCG TTTTCTCTTT C
          //
          Primer: SV40pro_F
          Sequence file: RDB16944_A9D8c.seq check
          >D06215A2_A9D8_2_SV40pro_F_G01_19_ABI24.ab1
              1 CCCTGGGTCC TGGGGCTTTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
             61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
            121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
            181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
            241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
            301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
            361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
            421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
            481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
            541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
            601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
            661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
            721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
            781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
            841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
            901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGCGAAATG ACCGACCAAG
            961 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CCGCCCTTCT ATGAAAGGTT
           1021 GGGCTTCGGA ATCGTTTTCC GGGGACGCCC GGCTGGGATG ATCCTCCAGC GCGGGGGATC
           1081 TCATGGCTGC AGTCCTCGCC CACCCCAAAC TTGGTTAATG CAGCTATATG CTAACAAATA
          1141 AAGCAAATAG CATCACCAAA TTTCCACCCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kikuchi, K., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl