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pcDNA3.1-V5His6-hMap7 (#RDB16944)

Expression vector of human microtubule associated protein 7 (MAP7) tagged with V5 and 6xHis.

Clone info. Expression vector of human microtubule associated protein 7 (MAP7) tagged with V5 and 6xHis at C-terminus.
Comment Expression was confirmed by the depositor with western blotting, immunofluorescence, immunoprecipitates. The cDNA encoding hMAP7 was provided by the RIKEN BRC (IRAK049L15, Cat No. HGX019879).
Vector backbone pcDNA3.1-V5His6 (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human MAP7 cDNA
Depositor|Developer Kikuchi, Koji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (EMBO Rep. 19 (7), pii: e45471, 2018.)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 (EMBO Rep. 19 (7), pii: e45471, 2018.)

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RDB16944 pcDNA3.1-V5His6-hMap7 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-V5His6-hMap7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16944).

Reference section:

Kikuchi, K., Nakamura, A., Arata, M., Shi, D., Nakagawa, M., Tanaka, T., Uemura, T., Fujimori, T., Kikuchi, A., Uezu, A., Sakamoto, Y., Nakanishi, H., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16944_A9D8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB16944_A9D8a.seq check
>D06215A2_A9D8_2_CMV_Forward_E01_13_ABI24.ab1
    1 CTGGTTAAAC TTCCGCCCCC ATTTGGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCGAGCTCGG
  181 ATCCATGGCG GAGCTAGGAG CTGGCGGCGA CGGCCACAGG GGCGGCGACG GCGCAGTGCG
  241 AAGCGAAACA GCACCCGACA GCTACAAAGT GCAAGATAAG AAAAATGCCT CCAGCCGCCC
  301 TGCCTCTGCA ATTTCAGGAC AAAATAACAA CCACTCAGGA AATAAACCAG ACCCTCCGCC
  361 TGTGTTACGT GTTGATGACC GGCAGCGGCT GGCCCGGGAG CGACGTGAGG AACGGGAGAA
  421 ACAGCTAGCT GCAAGAGAAA TAGTGTGGTT AGAAAGAGAA GAGCGAGCCA GGCAGCACTA
  481 CGAGAAGCAC CTGGAAGAGC GGAAGAAGAG GTTGGAGGAG CAGAGGCAGA AGGAGGAGCG
  541 GAGGAGGGCT GCTGTGGAGG AGAAGCGGAG GCAGAGACTT GAGGAGGACA AAGAACGCCA
  601 CGAAGCTGTT GTACGGCGCA CAATGGAAAG GAGCCAGAAG CCAAAACAGA AGCATAACCG
  661 TTGGTCGTGG GGAGGCTCTC TCCATGGGAG CCCTAGCATC CACAGTGCAG ATCCAGACAA
  721 GGCGGTCAGT TTCCACCATG AATCTTTCGA AAATATGTTG ATCCCGTCAT TAGCAAGCGG
  781 CTCTCCTCTT CATCTGCAAC TTTACTAAAT TCTCCAGATA GAAGCTCGCC GCCTGCAGCT
  841 CAGCCCATGG GGAGAGGCAG CGTTTGTTAA CAGACTCCTG ACGCCCACAC ATTCGTTTCC
  901 TGGGCCAGAA GTAAAAGCAC AGCTGCCTTG TCTGGGAGAA GCAGCATCTT TGCAGCCCCC
  961 ATCATCATGC CCCTACAAAG CTGCACACTT CTAGAAATTC GAATGGGATC GACCAAAACT
 1021 CTTTTGTTAA CACCACCCTG AAGGGCTCTT TCTTCGCCAA GGAGGGATCA ATTCATGGGC
 1081 ACAGCGAAGC TTATAAAAAA GAAAGAGGAG AGAGGAAATG TTACTCTCTT TCCTCTCACA
 1141 TCCTGGCACC CCGAAAGGGG CTGTATTCCT CATTCTAAAT TCCCAAGTCA GACACCGAGC
1201 TTCGGCCGTT GTC
//
Primer: f1ori_R3 ()
Sequence file: RDB16944_A9D8b.seq check
>D06215A2_A9D8_2_f1ori_R3_F01_16_ABI24.ab1
    1 GGGGGGGAAA CTGGAGCCCA GCTGGTTCTT TCCGCCTCAG AAGCCATAGA GCCCACCGCA
   61 TCCCCAGCAT GCCTGCTATT GTCTTCCCAA TCCTCCCCCT TGCTGTCCTG CCCCACCCCA
  121 CCCCCCAGAA TAGAATGACA CCTACTCAGA CAATGCGATG CAATTTCCTC ATTTTATTAG
  181 GAAAGGACAG TGGGAGTGGC ACCTTCCAGG GTCAAGGAAG GCACGGGGGA GGGGCAAACA
  241 ACAGATGGCT GGCAACTAGA AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACTCAATGG
  301 TGATGGTGAT GATGACCGGT ACGCGTAGAA TCGAGACCGA GGAGAGGGTT AGGGATAGGC
  361 TTACCTTCGA ACCGCGGGCC CTCTAGACTC GAGCCTATAA CTTCTGCAGT CTGCTGTGTC
  421 TGAACACCAT CTACCTGAGG CAGGGGCCCA AGTGTCCCTT CATCATCAAA GGCCAAAATT
  481 GGATTCAAAG GAATTTCTGG GCTCTCACTG TTGGTGACAT CTAATCTGGA TGGTTTAGAT
  541 CCAATGGGTA AGTTTATAAT TTCTTCAAAA TTTTCATTCT GAACAGATAC ACCATTTTCA
  601 TTTGGTTGTT TTTCCAAATC GGGAGTGCTC TCCACTGTCA CTTTTGACTG GTGTGAGGTA
  661 ACCACATGTG GGCTACCAAC TGGCTTTCCA TTTCCCGGAG CGTTTGTTGT ACATGGAAGT
  721 GCAGACACCT CTGTTCCTCC AGTGAGAGCT CCCTTGGCTA TATCACCGTT TCTCTGATCA
  781 CTGGTTTTCT TATCTGTAGC TTCTGTTCTC CTGGTTCTTT TCATAATCTC CTCAAGTCGC
  841 TTCTTTCTCT CCAGGCGCTC TTGCTCTTCT CTCTGGAAAT GCTTCTCTCG TTCCTGCCGG
  901 ACCCTCTCTG CTTCTTCACG AACGCGAGCT TCTTCTTCTT TCTGCCTCTG GGCGCGCTCT
  961 GCCTCCTCCC ACTCGCGCAG CGCCCGCTCC TCCGCCTGCC GCTGCAGCTG CTCCCTCCTT
 1021 TCTCCCGGCC TGCTCGGGCT TTCCAGCCTG CGCGACTCTC CTCACGGCGA GTCGGTCCTC
 1081 TCTTCAGCCA CACGTGGAGC CCACTCGTCT CTCATTTGGT CATTCCAGGC TCATTCTTGC
1141 CTTCCCGTCC CTCCTTCCCG TTTTCTCTTT C
//
Primer: SV40pro_F ()
Sequence file: RDB16944_A9D8c.seq check
>D06215A2_A9D8_2_SV40pro_F_G01_19_ABI24.ab1
    1 CCCTGGGTCC TGGGGCTTTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
  121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
  661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGCGAAATG ACCGACCAAG
  961 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CCGCCCTTCT ATGAAAGGTT
 1021 GGGCTTCGGA ATCGTTTTCC GGGGACGCCC GGCTGGGATG ATCCTCCAGC GCGGGGGATC
 1081 TCATGGCTGC AGTCCTCGCC CACCCCAAAC TTGGTTAATG CAGCTATATG CTAACAAATA
1141 AAGCAAATAG CATCACCAAA TTTCCACCCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kikuchi, K., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710. [link to RRC of NBRP]