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pEGFP-N3-hMap7 (#RDB16943)

Expression vector of human microtubule associated protein 7 (MAP7) fused with EGFP.

Clone info. Expression vector of human microtubule associated protein 7 (MAP7) fused with EGFP at C-terminus.
Comment Expression was confirmed by the depositor with western blotting, immunofluorescence, immunoprecipitates, live imaging. The cDNA encoding hMAP7 was provided by the RIKEN BRC (IRAK049L15, Cat No. HGX019879),
Vector backbone pEGFP-N3 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kam^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human MAP7 cDNA
Depositor|Developer Kikuchi, Koji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (EMBO Rep. 19 (7), pii: e45471, 2018.)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 (EMBO Rep. 19 (7), pii: e45471, 2018.)

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16943 pEGFP-N3-hMap7 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-N3-hMap7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16943).

Reference section:

Kikuchi, K., Nakamura, A., Arata, M., Shi, D., Nakagawa, M., Tanaka, T., Uemura, T., Fujimori, T., Kikuchi, A., Uezu, A., Sakamoto, Y., Nakanishi, H., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16943_A9D8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB16943_A9D8a.seq check
>D06215A1_A9D8_1_CMV_Forward_A01_01_ABI24.ab1
    1 TTGTTCACTC CGCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTATGG
  121 CGGAGCTAGG AGCTGGCGGC GACGGCCACA GGGGCGGCGA CGGCGCAGTG CGAAGCGAAA
  181 CAGCACCCGA CAGCTACAAA GTGCAAGATA AGAAAAATGC CTCCAGCCGC CCTGCCTCTG
  241 CAATTTCAGG ACAAAATAAC AACCACTCAG GAAATAAACC AGACCCTCCG CCTGTGTTAC
  301 GTGTTGATGA CCGGCAGCGG CTGGCCCGGG AGCGACGTGA GGAACGGGAG AAACAGCTAG
  361 CTGCAAGAGA AATAGTGTGG TTAGAAAGAG AAGAGCGAGC CAGGCAGCAC TACGAGAAGC
  421 ACCTGGAAGA GCGGAAGAAG AGGTTGGAGG AGCAGAGGCA GAAGGAGGAG CGGAGGAGGG
  481 CTGCTGTGGA GGAGAAGCGG AGGCAGAGAC TTGAGGAGGA CAAAGAACGC CACGAAGCTG
  541 TTGTACGGCG CACAATGGAA AGGAGCCAGA AGCCAAAACA GAAGCATAAC CGTTGGTCGT
  601 GGGGAGGCTC TCTCCATGGG AGCCCTAGCA TCCACAGTGC AGATCCAGAC AGGCGGTCAG
  661 TTTCCACCAT GAATCTTTCG AAATATGTTG ATCCCGTCAT TAGCAAGCGG CTCTCCTCTT
  721 CATCTGCAAC TTTACTAAAT TCTCCAGATA GAGCTCGCCG CCTGCAGCTC AGCCCATGGG
  781 AGAGCAGCGT TGTTAACAGA CTCCTGACGC CCACACATTC GTTCCTGGCC AGAAGTAAAA
  841 GCACAGCTGC CTTGTCTGGA GAAGCAGCAT CTTGCAGCCC CATCATCATG CCCTACAAAG
  901 CTGCACACTC TAGAAATTCG ATGGATCGAC CAAAACTCTT TGTAACACCA CCTGAGGGCT
  961 CTTCTCGCAG GAGGATCATT CATGGCACAG CGAGCTATAA AAAAAGAAAG AGAGAGAGAA
 1021 AATGTACTCT TTCCTCACAT CTGGGCACCC CGAAGGGCTG TATCTCCAAT CTATTCCCTA
 1081 AAGCAGACAC CCAGCTCGCC TCCCGGACTT GCCTCGCTCA AGGTCTTTTC CTCCAGTTGG
1141 CCTGTGGCAC AAC
//
Primer: EGFP-N ()
Sequence file: RDB16943_A9D8b.seq check
>D06215A1_A9D8_1_EGFP-N_B01_04_ABI24.ab1
    1 GAAACCCCGG GGGGGACAGC TCCTCGCCTT GCTCACCATG GTGGCGATGG ATCCCGGGCC
   61 CGCGGTACCG TCGACTATAA CTTCTGCAGT CTGCTGTGTC TGAACACCAT CTACCTGAGG
  121 CAGGGGCCCA AGTGTCCCTT CATCATCAAA GGCCAAAATT GGATTCAAAG GAATTTCTGG
  181 GCTCTCACTG TTGGTGACAT CTAATCTGGA TGGTTTAGAT CCAATGGGTA AGTTTATAAT
  241 TTCTTCAAAA TTTTCATTCT GAACAGATAC ACCATTTTCA TTTGGTTGTT TTTCCAAATC
  301 GGGAGTGCTC TCCACTGTCA CTTTTGACTG GTGTGAGGTA ACCACATGTG GGCTACCAAC
  361 TGGCTTTCCA TTTCCCGGAG CGTTTGTTGT ACATGGAAGT GCAGACACCT CTGTTCCTCC
  421 AGTGAGAGCT CCCTTGGCTA TATCACCGTT TCTCTGATCA CTGGTTTTCT TATCTGTAGC
  481 TTCTGTTCTC CTGGTTCTTT TCATAATCTC CTCAAGTCGC TTCTTTCTCT CCAGGCGCTC
  541 TTGCTCTTCT CTCTGGAAAT GCTTCTCTCG TTCCTGCCGG ACCCTCTCTG CTTCTTCACG
  601 AACGCGAGCT TCTTCTTCTT TCTGCCTCTG GGCGCGCTCT GCCTCCTCCC ACTCGCGCAG
  661 CGCCCGCTCC TCCGCCTGCC GCTGCAGCTG CTCCTCCTTC TCCCGGGCCT GCTCGGCTTC
  721 CAGCCTGCGC GACTCCTCCT CACGGCGAGT CGTCCTCTCT TCAGCCACAC GTTGAGCCAA
  781 TTCCTCTCTC TTTTGTCTTT CAAGCTCTTC CTGCTCCCTC CTCTCCCTTT CTTCCTTTTC
  841 TCTCTGCTCT CGGGCCAGCC GCCTCTTCTC AGCTAGAAGC CTTGTGGCCT CCTCTGGGTC
  901 GGTGGTGCCT GCAGAAGTCT TAACAGAAGC ACTGGCATTC ACAGTGGGAT GACGGGGCTG
  961 AGACCATGGC TGGGGTGGGG ACCGGGCTGG AGCTGGGGCC GAGCTGGAGC TGGGGCCGAG
 1021 GCTGGAGCTG GGGCCATGGC TGGAGCAGCA AGGGCCAAAC TTCTGGTTTC AGCAGTGTCC
 1081 GCCTCCTTCA CCTGTGGCTC TCTACCTTCA CTAAGTGCTC TGCCTTATTG AAGCTCATGC
1141 CAACTTTCTG AAGTTCCTCC TCAAGGGATC CTCTTCCT
//
Primer: ME-1290RV ()
Sequence file: RDB16943_A9D8c.seq check
>D06215A1_A9D8_1_ME-1290RV_C01_07_ABI24.ab1
    1 AGGGCAATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
   61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTTACTTG
  121 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
  181 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGGTGCTCAG GTAGTGGTTG
  241 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
  301 TGCACGCTGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TCACCTTGAT GCCGTTCTTC
  361 TGCTTGTCGG CCATGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC
  421 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG
  481 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG
  541 GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG
  601 TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC GAGGGTGGGC
  661 CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTAGGTG
  721 GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC
  781 TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG
  841 ATGGATCCCG GGCCCGCGGT ACCGTCGACT ATAACTTCTG CAGTCTGCTG TGTCTGAACA
  901 CCATCTACCT GAGGCAGGGG CCCAAGTGTC CCTTCATCAT CAAAGGCCAA AATTGGATTC
  961 AAAGGAATTT CTGGGCTCTC ACTGTTGGTG ACATCTAATC TGGATGGTTT AGATCCAATG
 1021 GGTAAGTTTA TAATTTCTTC AAATTTTCAT CTGACAGATA CACATTTTCA TTTGGTTGAT
 1081 TTTCGAATTC GGGAGTGCTC TCACTGGTCA CTTTTGGAAC TTGATGTAGA TACGACAGGT
1141 GCTACTACTG CATTCCTATT TTG
//
Primer: SV40pro_F ()
Sequence file: RDB16943_A9D8d.seq check
>D06215A1_A9D8_1_SV40pro_F_D01_10_ABI24.ab1
    1 CCCCCGGCCG TTTAAACTTT TCAGAAGGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
   61 TTGCAAAGAT CGATCAAGAG ACAGGTATGA GGATCGTTTC GCATGATTGA ACAAGATGGA
  121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
  181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
  241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG
  301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
  361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC
  421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
  481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
  541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG
  601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG
  661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC
  721 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT
  781 GATATTGCTG AAGAGCTTGG CGGCGAATGG GGCTGACCGC TTCCTCGTGC TTTACGGGTA
  841 TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG
  901 CGGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCCTGCC ATCACGAGAT
  961 TTCGATTTCC ACCCGCCCGC CTTCTAATGA AAGGTTGGGG CTTCGGAATC GTTTTCCGGG
 1021 ACGCCCGGCT GGGATGATCC TCCCAGCGCG GGGATCTCAT GCTTGGAAGT CTTTCGCCCC
 1081 ACCCCTAGGG GGGAGGCCTA ACTGAAACAT CGAGGGAGAC CAATTACCGG AAGGACCCGC
1141 GCTATGACGC CATAAAGGAC GGAATTAAAA CGCCCACGCG GGTGTTGTGG GGTCTCGCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kikuchi, K., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710. [link to RRC of NBRP]