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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-N3-hMap7

Expression vector of human microtubule associated protein 7 (MAP7) fused with EGFP.

Catalog number RDB16943
Resource name pEGFP-N3-hMap7
Clone info. Expression vector of human microtubule associated protein 7 (MAP7) fused with EGFP at C-terminus.
Comment Expression was confirmed by the depositor with western blotting, immunofluorescence, immunoprecipitates, live imaging. The cDNA encoding hMAP7 was provided by the RIKEN BRC (IRAK049L15, Cat No. HGX019879),
Vector backbone pEGFP-N3 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kam^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human MAP7 cDNA
Depositor|Developer Kikuchi, Koji |
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External Database
human MAP7

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (EMBO Rep. 19 (7), pii: e45471, 2018.)
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 (EMBO Rep. 19 (7), pii: e45471, 2018.)
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16943 pEGFP-N3-hMap7 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kikuchi, K., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0100e (English text)
          Featured content Dnaconda's recommendation EXR0100j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16943_A9D8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB16943_A9D8a.seq check
          >D06215A1_A9D8_1_CMV_Forward_A01_01_ABI24.ab1
              1 TTGTTCACTC CGCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTATGG
            121 CGGAGCTAGG AGCTGGCGGC GACGGCCACA GGGGCGGCGA CGGCGCAGTG CGAAGCGAAA
            181 CAGCACCCGA CAGCTACAAA GTGCAAGATA AGAAAAATGC CTCCAGCCGC CCTGCCTCTG
            241 CAATTTCAGG ACAAAATAAC AACCACTCAG GAAATAAACC AGACCCTCCG CCTGTGTTAC
            301 GTGTTGATGA CCGGCAGCGG CTGGCCCGGG AGCGACGTGA GGAACGGGAG AAACAGCTAG
            361 CTGCAAGAGA AATAGTGTGG TTAGAAAGAG AAGAGCGAGC CAGGCAGCAC TACGAGAAGC
            421 ACCTGGAAGA GCGGAAGAAG AGGTTGGAGG AGCAGAGGCA GAAGGAGGAG CGGAGGAGGG
            481 CTGCTGTGGA GGAGAAGCGG AGGCAGAGAC TTGAGGAGGA CAAAGAACGC CACGAAGCTG
            541 TTGTACGGCG CACAATGGAA AGGAGCCAGA AGCCAAAACA GAAGCATAAC CGTTGGTCGT
            601 GGGGAGGCTC TCTCCATGGG AGCCCTAGCA TCCACAGTGC AGATCCAGAC AGGCGGTCAG
            661 TTTCCACCAT GAATCTTTCG AAATATGTTG ATCCCGTCAT TAGCAAGCGG CTCTCCTCTT
            721 CATCTGCAAC TTTACTAAAT TCTCCAGATA GAGCTCGCCG CCTGCAGCTC AGCCCATGGG
            781 AGAGCAGCGT TGTTAACAGA CTCCTGACGC CCACACATTC GTTCCTGGCC AGAAGTAAAA
            841 GCACAGCTGC CTTGTCTGGA GAAGCAGCAT CTTGCAGCCC CATCATCATG CCCTACAAAG
            901 CTGCACACTC TAGAAATTCG ATGGATCGAC CAAAACTCTT TGTAACACCA CCTGAGGGCT
            961 CTTCTCGCAG GAGGATCATT CATGGCACAG CGAGCTATAA AAAAAGAAAG AGAGAGAGAA
           1021 AATGTACTCT TTCCTCACAT CTGGGCACCC CGAAGGGCTG TATCTCCAAT CTATTCCCTA
           1081 AAGCAGACAC CCAGCTCGCC TCCCGGACTT GCCTCGCTCA AGGTCTTTTC CTCCAGTTGG
          1141 CCTGTGGCAC AAC
          //
          Primer: EGFP-N
          Sequence file: RDB16943_A9D8b.seq check
          >D06215A1_A9D8_1_EGFP-N_B01_04_ABI24.ab1
              1 GAAACCCCGG GGGGGACAGC TCCTCGCCTT GCTCACCATG GTGGCGATGG ATCCCGGGCC
             61 CGCGGTACCG TCGACTATAA CTTCTGCAGT CTGCTGTGTC TGAACACCAT CTACCTGAGG
            121 CAGGGGCCCA AGTGTCCCTT CATCATCAAA GGCCAAAATT GGATTCAAAG GAATTTCTGG
            181 GCTCTCACTG TTGGTGACAT CTAATCTGGA TGGTTTAGAT CCAATGGGTA AGTTTATAAT
            241 TTCTTCAAAA TTTTCATTCT GAACAGATAC ACCATTTTCA TTTGGTTGTT TTTCCAAATC
            301 GGGAGTGCTC TCCACTGTCA CTTTTGACTG GTGTGAGGTA ACCACATGTG GGCTACCAAC
            361 TGGCTTTCCA TTTCCCGGAG CGTTTGTTGT ACATGGAAGT GCAGACACCT CTGTTCCTCC
            421 AGTGAGAGCT CCCTTGGCTA TATCACCGTT TCTCTGATCA CTGGTTTTCT TATCTGTAGC
            481 TTCTGTTCTC CTGGTTCTTT TCATAATCTC CTCAAGTCGC TTCTTTCTCT CCAGGCGCTC
            541 TTGCTCTTCT CTCTGGAAAT GCTTCTCTCG TTCCTGCCGG ACCCTCTCTG CTTCTTCACG
            601 AACGCGAGCT TCTTCTTCTT TCTGCCTCTG GGCGCGCTCT GCCTCCTCCC ACTCGCGCAG
            661 CGCCCGCTCC TCCGCCTGCC GCTGCAGCTG CTCCTCCTTC TCCCGGGCCT GCTCGGCTTC
            721 CAGCCTGCGC GACTCCTCCT CACGGCGAGT CGTCCTCTCT TCAGCCACAC GTTGAGCCAA
            781 TTCCTCTCTC TTTTGTCTTT CAAGCTCTTC CTGCTCCCTC CTCTCCCTTT CTTCCTTTTC
            841 TCTCTGCTCT CGGGCCAGCC GCCTCTTCTC AGCTAGAAGC CTTGTGGCCT CCTCTGGGTC
            901 GGTGGTGCCT GCAGAAGTCT TAACAGAAGC ACTGGCATTC ACAGTGGGAT GACGGGGCTG
            961 AGACCATGGC TGGGGTGGGG ACCGGGCTGG AGCTGGGGCC GAGCTGGAGC TGGGGCCGAG
           1021 GCTGGAGCTG GGGCCATGGC TGGAGCAGCA AGGGCCAAAC TTCTGGTTTC AGCAGTGTCC
           1081 GCCTCCTTCA CCTGTGGCTC TCTACCTTCA CTAAGTGCTC TGCCTTATTG AAGCTCATGC
          1141 CAACTTTCTG AAGTTCCTCC TCAAGGGATC CTCTTCCT
          //
          Primer: ME-1290RV
          Sequence file: RDB16943_A9D8c.seq check
          >D06215A1_A9D8_1_ME-1290RV_C01_07_ABI24.ab1
              1 AGGGCAATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
             61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTTACTTG
            121 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
            181 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGGTGCTCAG GTAGTGGTTG
            241 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
            301 TGCACGCTGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TCACCTTGAT GCCGTTCTTC
            361 TGCTTGTCGG CCATGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC
            421 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG
            481 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG
            541 GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG
            601 TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC GAGGGTGGGC
            661 CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTAGGTG
            721 GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC
            781 TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG
            841 ATGGATCCCG GGCCCGCGGT ACCGTCGACT ATAACTTCTG CAGTCTGCTG TGTCTGAACA
            901 CCATCTACCT GAGGCAGGGG CCCAAGTGTC CCTTCATCAT CAAAGGCCAA AATTGGATTC
            961 AAAGGAATTT CTGGGCTCTC ACTGTTGGTG ACATCTAATC TGGATGGTTT AGATCCAATG
           1021 GGTAAGTTTA TAATTTCTTC AAATTTTCAT CTGACAGATA CACATTTTCA TTTGGTTGAT
           1081 TTTCGAATTC GGGAGTGCTC TCACTGGTCA CTTTTGGAAC TTGATGTAGA TACGACAGGT
          1141 GCTACTACTG CATTCCTATT TTG
          //
          Primer: SV40pro_F
          Sequence file: RDB16943_A9D8d.seq check
          >D06215A1_A9D8_1_SV40pro_F_D01_10_ABI24.ab1
              1 CCCCCGGCCG TTTAAACTTT TCAGAAGGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
             61 TTGCAAAGAT CGATCAAGAG ACAGGTATGA GGATCGTTTC GCATGATTGA ACAAGATGGA
            121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
            181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
            241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG
            301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
            361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC
            421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
            481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
            541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG
            601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG
            661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC
            721 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT
            781 GATATTGCTG AAGAGCTTGG CGGCGAATGG GGCTGACCGC TTCCTCGTGC TTTACGGGTA
            841 TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG
            901 CGGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCCTGCC ATCACGAGAT
            961 TTCGATTTCC ACCCGCCCGC CTTCTAATGA AAGGTTGGGG CTTCGGAATC GTTTTCCGGG
           1021 ACGCCCGGCT GGGATGATCC TCCCAGCGCG GGGATCTCAT GCTTGGAAGT CTTTCGCCCC
           1081 ACCCCTAGGG GGGAGGCCTA ACTGAAACAT CGAGGGAGAC CAATTACCGG AAGGACCCGC
          1141 GCTATGACGC CATAAAGGAC GGAATTAAAA CGCCCACGCG GGTGTTGTGG GGTCTCGCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kikuchi, K., Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling. EMBO Rep. 19 (7). pii: e45471 (2018). PMID 29880710.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl