Please review the QC test results indicated by icon below as well as clone information before placing your order.

phRIT2

Lentiviral expression vector of human RIT2.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Uenaka, T. et al. Mol. Genet. 27 (22), 3974-3985, 2018) Additional terms and conditions: The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Uenaka, T. et al. Mol. Genet. 27 (22), 3974-3985, 2018) 付加的提供条件： 本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16941_A9Cqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_v2_F () Sequence file: RDB16941_A9Cqa.seq
>D06248A1_A9Cq_1_cPPT+CTS_v2_F_A01_01_ABI08.ab1     1 GTTAGGCATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT    61 TTATTACAGG GACAGCAGAA ATTCACTTTG AATTAATTCG ATTAATAGTA ATCAATTACG   121 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC   181 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC   241 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT   301 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT   361 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT   421 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC   481 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC   541 GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC   601 TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA   661 GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCCGCCACC ATGGAGGTAG AAAATGAAGC   721 CAGCTGCTCC CCGGGCAGCG CATCAGGCGG GTCCAGAGAG TACAAGGTGG TAATGCTGGG   781 AGCAGGGGGA GTTGGTAAAA GCGCAATGAC AATGCAGTTT ATTAGTCATC AGTTCCCTGA   841 TTATCATGAC CCTACTATAG AAGATGCTTA TAAGACCCAG GTCAGGATTG ACAATGAGCC   901 AGCTTACTTG GACATCTTGG ACACTGCTGG CCAGGCAGAA TTCACAGCCA TGCGGGAGCA   961 GTACATGCGA GGTGGGAAGG CTTCATCATC TGCTACTCCG TCACTGACCG TCAATCATTT  1021 CAGGAGGCTG CTAAGTTTAA GGAGCTCATT TTCCAGGTCG TCCACACTAA TGAAATCCCC 1081 TGGATGCTGC TGGGCTTAAC CGAAAATTGA TTCCTGGGAA CACAG //
Primer: IRES-R () Sequence file: RDB16941_A9Cqb.seq
>D06248A1_A9Cq_1_IRES-R_B01_02_ABI08.ab1     1 GATTTCGGGG GGCTTTTCGG CCCGTACCGG TTAGGGGGGG GGGGAGGGAG AGGGGCGGAT    61 CCGCGGCCGC TCATGTCATA TTTTCTCTCT TCTTCTTCAA AGAACCTTTG AGCTTCTTCC   121 ACAGGCTGTC TTTTCTCTTC AGTTTCTTTT CCATCAAGGA TGGCATGGAC TCCTTCTTGC   181 GAATTTCCCT CACTAAGCCA TGAAAAGCAT CATCAATACA GAATCTGAGG GCTGCAGAGG   241 TCTCAAAAAA ACCACAATTA TATTCTTGGG CAAGACTCAA GCCTTCTTCT GTAGAAACCT   301 GGCGGAACTG TTCCAGATCA ATTTTGTTAC CCACCAGCAC CAGGGGAATT TCATAGGTGT   361 GGCGGACCTG AAAAATGAGC TCTTTAAACT TGGCAGCCTC CTGAAATGAT TGACGGTCAG   421 TGACGGAGTA GCAGATGATG AAGCCTTCCC CACCTCGCAT GTACTGCTCC CGCATGGCTG   481 TGAATTCTGC CTGGCCAGCA GTGTCCAAGA TGTCCAAGTA AGCTGGCTCA TTGTCAATCC   541 TGACCTGGGT CTTATAAGCA TCTTCTATAG TAGGGTCATG ATAATCAGGG AACTGATGAC   601 TAATAAACTG CATTGTCATT GCGCTTTTAC CAACTCCCCC TGCTCCCAGC ATTACCACCT   661 TGTACTCTCT GGACCCGCCT GATGCGCTGC CCGGGGAGCA GCTGGCTTCA TTTTCTACCT   721 CCATGGTGGC GGCGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT TATATAGACC   781 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT   841 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGG   901 ACTTGGAAAA TCCCGTGAGT CAAAACCGCT ATCCACGCCC ATTGATGTAC TGCCAAAACC   961 GCAATCACCA ATGGGTAATA GCGATGACTA AATACGTAGA ATGTACTGGC CCAAGTAGGA  1021 AGTTCCCATT AAGGTCATGT ACTTGGGGCA TAATGCAGCC GGCAATTTAA CCCGTCATTT  1081 GACCGTCAAT AAGGGGGGCG GTACTTGGCA TATGAATACC CTTGGAATGT ACTTGCAAAG 1141 TGGCCAGGTT TAACCGGATT A //
Primer: HIVgp_R1 () Sequence file: RDB16941_A9Cqc.seq
>D06248A1_A9Cq_1_HIVgp_R1_C01_03_ABI08.ab1     1 CCGGGCCTTC TATTCCCACT GCTCTTTTTT CTCTCTGCAC CACTCTTCTC TTTGCCTTGG    61 TGGGTGCTAC TCCTAATGGT TCAATTTTTA CTACTTTATA TTTATATAAT TCACTTCTCC   121 AATTGTCCCT CATATCTCCT CCTCCAGGTC TGAAGATCAG CGGCCGGCCG CTTGCTGTGC   181 GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC TATCTTGTCT AAAGCTTCCT TGGTGTCTTT   241 TATCTCTATC CTTTGATGCA CACAATAGAG GGTTGCTACT GTATTATATA ATGATCTAAG   301 TTCTTCTGAT CCTGTCTGAA GGGATGGTTG TAGCTGTCCC AGTATTTGTC TACAGCCTTC   361 TGATGTTTCT AACAGGCCAG GATTAACTGC GAATCGTTCT AGCTCCCTGC TTGCCCATAC   421 TATATGTTTT AATTTATATT TTTTCTTTCC CCCTGGCCTT AACCGAATTT TTTCCCATCG   481 CGATCTAATT CTCCCCCGCT TAATACTGAC GCTCTCGCAC CCATCTCTCT CCTTCTAGCC   541 TCCGCTAGTC AAAATTTTTG GCGTACTCAC CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG   601 CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA   661 GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT   721 CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG   781 CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC   841 TGGTCTAACC AGAGAGACCC AGTACAGGCA AAACGCGCTG CTTATATAGA CCTCCCACCG   901 TACACGCCTA CCGCCCATTT GCGTCAATGG GGGCGGAGTT GTTACGACAT TTTGGGAAAG   961 TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTTGG  1021 AATCCCCGTG AGTCCAACCC GCTATCCACG CCCAATTGAT GTACTGCCGA AACGCATCAC  1081 CATGGTAATA GCGAATGACT ATACGTTAGA TGTACCTGCC AAGGTAGGAA AGGTTCCCCA 1141 ATTATAAA //
Primer: IRES-F () Sequence file: RDB16941_A9Cqd.seq
>D06248A1_A9Cq_1_IRES-F_D01_04_ABI08.ab1     1 TGTTTCCTTT TCATGGTGTT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC    61 GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACAACCA TGGTGAGCAA   121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA   181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC   241 CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC   301 CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT   361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA   421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT   481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA   541 CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC   601 CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA   661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA   721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT   781 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAG AATTCAAGGC   841 CTCTCGAGCC TCTAGAGTTA ACATCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT   901 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TTACGCTATG   961 TGGATACGCT GCTTTTAATG CCTTTGTATC ATGCTATTGC TTTCCCGTAT GGGCTTTCAT  1021 TTTCTCCTCC TTGGTAATAA ATCCTGGTTG GCTGGTTCTT CTTTTATGAG GGAGGTGATG  1081 GGCCGTTGTT CAGGCAACGT GCCGTTGGTG TTGCCACTGT GATTTGGCTT GAACGCCAAA 1141 CTCTC //
Primer: BGH_rev () Sequence file: RDB16941_A9Cqe.seq
>D06248A1_A9Cq_1_BGH_rev_E01_05_ABI08.ab1     1 GTTTAACGGG GGGCTTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT    61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT   121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT   181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA   241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC   301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT   361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG   421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA   481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT   541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG   601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG   661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG   721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC   781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT   841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG   901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC   961 AGCAGCGGGG CAAGGCAGGC GGGCGATGAG TTTCCGGCCG TGGCAATAAG GGAGGGGGGA  1021 AAGCGAAAGT CCCGGGAAAG GAGCTGAACA GTGGTGGGCA ATGCCCCATC AGTGGGGGGG  1081 ATGCGTTCAG CCAAACACAG TGCCACACTA GTCACGTGCC TGGACAACGG GGCCACAACT 1141 TCTTCAATTA GGAGAACTTG CCGAC //
Primer: SV40pro_F () Sequence file: RDB16941_A9Cqf.seq
>D06248A1_A9Cq_1_SV40pro_F_F01_06_ABI08.ab1     1 CCTCGGGGCT TGGGGCTTTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT   121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG   181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT   241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG   301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA   361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG   421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT   481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG   541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG   601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC   661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA   721 ATAGCATCAC AAATTTCACA AATAAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGGTTT   781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT   841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA   901 CAAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG GTGCCCTAAT GAGTGAGCTA   961 ACTCACATTA AATTGCGTTG GCGCTTCACT GGCCCCGCTT TTCCAGTCGG GGAAACCCTG  1021 TCGTGCCAGC TGCATTTAAT GAAATCGGGC CAACGCGCCG GGAAGAGGCC GAATTTGGCG  1081 GTATTGGGGC CGCTCTTCCG GCTTCGTCGG CTTCACTGGA CTCCGCTGCG CCTCGGGTTC 1141 GATTTCAGAC CTGCGAGCGG AAGACGGGAT TATTCACGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl