Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

phRIT2 (#RDB16941)

Lentiviral expression vector of human RIT2.

Alternative name CSII-CMV-hRIT2-IRES2-Venus
Clone info. Lentiviral expression vector of human Ras-like without CAAX 2 (RIT2), CMV promoter.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone CSII-CMV-MCS-IRES2-Venus (Plasmid)
Size of vector backbone 9.9 kb
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Human RIT2 cDNA
Depositor|Developer Toda, Tatsushi | Uenaka, Takeshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Uenaka, T. et al. Mol. Genet. 27 (22), 3974-3985, 2018)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Uenaka, T. et al. Mol. Genet. 27 (22), 3974-3985, 2018)
付加的使用条件：
本件研究材料は、非営利機関の非営利学術研究に限って提供する。
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16941 phRIT2 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The phRIT2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16941).

Reference section:

Uenaka, T., Satake, W., Cha, P.C., Hayakawa, H., Baba, K., Jiang, S., Kobayashi, K., Kanagawa, M., Okada, Y., Mochizuki, H., Toda, T., In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum. Mol. Genet. 27 (22): 3974-3985 (2018). PMID 30137437. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16941_A9Cqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_v2_F ()
Sequence file: RDB16941_A9Cqa.seq check
>D06248A1_A9Cq_1_cPPT+CTS_v2_F_A01_01_ABI08.ab1
    1 GTTAGGCATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT
   61 TTATTACAGG GACAGCAGAA ATTCACTTTG AATTAATTCG ATTAATAGTA ATCAATTACG
  121 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
  181 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
  241 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
  301 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
  361 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT
  421 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC
  481 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC
  541 GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC
  601 TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA
  661 GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCCGCCACC ATGGAGGTAG AAAATGAAGC
  721 CAGCTGCTCC CCGGGCAGCG CATCAGGCGG GTCCAGAGAG TACAAGGTGG TAATGCTGGG
  781 AGCAGGGGGA GTTGGTAAAA GCGCAATGAC AATGCAGTTT ATTAGTCATC AGTTCCCTGA
  841 TTATCATGAC CCTACTATAG AAGATGCTTA TAAGACCCAG GTCAGGATTG ACAATGAGCC
  901 AGCTTACTTG GACATCTTGG ACACTGCTGG CCAGGCAGAA TTCACAGCCA TGCGGGAGCA
  961 GTACATGCGA GGTGGGAAGG CTTCATCATC TGCTACTCCG TCACTGACCG TCAATCATTT
 1021 CAGGAGGCTG CTAAGTTTAA GGAGCTCATT TTCCAGGTCG TCCACACTAA TGAAATCCCC
1081 TGGATGCTGC TGGGCTTAAC CGAAAATTGA TTCCTGGGAA CACAG
//
Primer: IRES-R ()
Sequence file: RDB16941_A9Cqb.seq check
>D06248A1_A9Cq_1_IRES-R_B01_02_ABI08.ab1
    1 GATTTCGGGG GGCTTTTCGG CCCGTACCGG TTAGGGGGGG GGGGAGGGAG AGGGGCGGAT
   61 CCGCGGCCGC TCATGTCATA TTTTCTCTCT TCTTCTTCAA AGAACCTTTG AGCTTCTTCC
  121 ACAGGCTGTC TTTTCTCTTC AGTTTCTTTT CCATCAAGGA TGGCATGGAC TCCTTCTTGC
  181 GAATTTCCCT CACTAAGCCA TGAAAAGCAT CATCAATACA GAATCTGAGG GCTGCAGAGG
  241 TCTCAAAAAA ACCACAATTA TATTCTTGGG CAAGACTCAA GCCTTCTTCT GTAGAAACCT
  301 GGCGGAACTG TTCCAGATCA ATTTTGTTAC CCACCAGCAC CAGGGGAATT TCATAGGTGT
  361 GGCGGACCTG AAAAATGAGC TCTTTAAACT TGGCAGCCTC CTGAAATGAT TGACGGTCAG
  421 TGACGGAGTA GCAGATGATG AAGCCTTCCC CACCTCGCAT GTACTGCTCC CGCATGGCTG
  481 TGAATTCTGC CTGGCCAGCA GTGTCCAAGA TGTCCAAGTA AGCTGGCTCA TTGTCAATCC
  541 TGACCTGGGT CTTATAAGCA TCTTCTATAG TAGGGTCATG ATAATCAGGG AACTGATGAC
  601 TAATAAACTG CATTGTCATT GCGCTTTTAC CAACTCCCCC TGCTCCCAGC ATTACCACCT
  661 TGTACTCTCT GGACCCGCCT GATGCGCTGC CCGGGGAGCA GCTGGCTTCA TTTTCTACCT
  721 CCATGGTGGC GGCGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT TATATAGACC
  781 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT
  841 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGG
  901 ACTTGGAAAA TCCCGTGAGT CAAAACCGCT ATCCACGCCC ATTGATGTAC TGCCAAAACC
  961 GCAATCACCA ATGGGTAATA GCGATGACTA AATACGTAGA ATGTACTGGC CCAAGTAGGA
 1021 AGTTCCCATT AAGGTCATGT ACTTGGGGCA TAATGCAGCC GGCAATTTAA CCCGTCATTT
 1081 GACCGTCAAT AAGGGGGGCG GTACTTGGCA TATGAATACC CTTGGAATGT ACTTGCAAAG
1141 TGGCCAGGTT TAACCGGATT A
//
Primer: HIVgp_R1 ()
Sequence file: RDB16941_A9Cqc.seq check
>D06248A1_A9Cq_1_HIVgp_R1_C01_03_ABI08.ab1
    1 CCGGGCCTTC TATTCCCACT GCTCTTTTTT CTCTCTGCAC CACTCTTCTC TTTGCCTTGG
   61 TGGGTGCTAC TCCTAATGGT TCAATTTTTA CTACTTTATA TTTATATAAT TCACTTCTCC
  121 AATTGTCCCT CATATCTCCT CCTCCAGGTC TGAAGATCAG CGGCCGGCCG CTTGCTGTGC
  181 GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC TATCTTGTCT AAAGCTTCCT TGGTGTCTTT
  241 TATCTCTATC CTTTGATGCA CACAATAGAG GGTTGCTACT GTATTATATA ATGATCTAAG
  301 TTCTTCTGAT CCTGTCTGAA GGGATGGTTG TAGCTGTCCC AGTATTTGTC TACAGCCTTC
  361 TGATGTTTCT AACAGGCCAG GATTAACTGC GAATCGTTCT AGCTCCCTGC TTGCCCATAC
  421 TATATGTTTT AATTTATATT TTTTCTTTCC CCCTGGCCTT AACCGAATTT TTTCCCATCG
  481 CGATCTAATT CTCCCCCGCT TAATACTGAC GCTCTCGCAC CCATCTCTCT CCTTCTAGCC
  541 TCCGCTAGTC AAAATTTTTG GCGTACTCAC CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG
  601 CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA
  661 GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT
  721 CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG
  781 CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC
  841 TGGTCTAACC AGAGAGACCC AGTACAGGCA AAACGCGCTG CTTATATAGA CCTCCCACCG
  901 TACACGCCTA CCGCCCATTT GCGTCAATGG GGGCGGAGTT GTTACGACAT TTTGGGAAAG
  961 TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTTGG
 1021 AATCCCCGTG AGTCCAACCC GCTATCCACG CCCAATTGAT GTACTGCCGA AACGCATCAC
 1081 CATGGTAATA GCGAATGACT ATACGTTAGA TGTACCTGCC AAGGTAGGAA AGGTTCCCCA
1141 ATTATAAA
//
Primer: IRES-F ()
Sequence file: RDB16941_A9Cqd.seq check
>D06248A1_A9Cq_1_IRES-F_D01_04_ABI08.ab1
    1 TGTTTCCTTT TCATGGTGTT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC
   61 GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACAACCA TGGTGAGCAA
  121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
  181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
  241 CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
  301 CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT
  361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
  421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
  481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
  541 CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC
  601 CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA
  661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA
  721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
  781 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAG AATTCAAGGC
  841 CTCTCGAGCC TCTAGAGTTA ACATCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT
  901 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TTACGCTATG
  961 TGGATACGCT GCTTTTAATG CCTTTGTATC ATGCTATTGC TTTCCCGTAT GGGCTTTCAT
 1021 TTTCTCCTCC TTGGTAATAA ATCCTGGTTG GCTGGTTCTT CTTTTATGAG GGAGGTGATG
 1081 GGCCGTTGTT CAGGCAACGT GCCGTTGGTG TTGCCACTGT GATTTGGCTT GAACGCCAAA
1141 CTCTC
//
Primer: BGH_rev ()
Sequence file: RDB16941_A9Cqe.seq check
>D06248A1_A9Cq_1_BGH_rev_E01_05_ABI08.ab1
    1 GTTTAACGGG GGGCTTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
   61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA
  241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC
  301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT
  361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG
  421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA
  481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT
  541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG
  601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG
  661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG
  721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC
  781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT
  841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG
  901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC
  961 AGCAGCGGGG CAAGGCAGGC GGGCGATGAG TTTCCGGCCG TGGCAATAAG GGAGGGGGGA
 1021 AAGCGAAAGT CCCGGGAAAG GAGCTGAACA GTGGTGGGCA ATGCCCCATC AGTGGGGGGG
 1081 ATGCGTTCAG CCAAACACAG TGCCACACTA GTCACGTGCC TGGACAACGG GGCCACAACT
1141 TCTTCAATTA GGAGAACTTG CCGAC
//
Primer: SV40pro_F ()
Sequence file: RDB16941_A9Cqf.seq check
>D06248A1_A9Cq_1_SV40pro_F_F01_06_ABI08.ab1
    1 CCTCGGGGCT TGGGGCTTTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGGTTT
  781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT
  841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
  901 CAAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG GTGCCCTAAT GAGTGAGCTA
  961 ACTCACATTA AATTGCGTTG GCGCTTCACT GGCCCCGCTT TTCCAGTCGG GGAAACCCTG
 1021 TCGTGCCAGC TGCATTTAAT GAAATCGGGC CAACGCGCCG GGAAGAGGCC GAATTTGGCG
 1081 GTATTGGGGC CGCTCTTCCG GCTTCGTCGG CTTCACTGGA CTCCGCTGCG CCTCGGGTTC
1141 GATTTCAGAC CTGCGAGCGG AAGACGGGAT TATTCACGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Uenaka, T., In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum. Mol. Genet. 27 (22): 3974-3985 (2018). PMID 30137437. [link to RRC of NBRP]