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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

phRIT2

Lentiviral expression vector of human RIT2.

Catalog number RDB16941
Resource name phRIT2
Alternative name CSII-CMV-hRIT2-IRES2-Venus
Clone info. Lentiviral expression vector of human Ras-like without CAAX 2 (RIT2), CMV promoter.
Comment Expression was confirmed by the depositor with western blotting. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS-IRES2-Venus (Plasmid)
Size of vector backbone 9.9 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human RIT2 cDNA
Depositor|Developer Toda, Tatsushi | Uenaka, Takeshi |
Other clones in our bank

External Database
Human RIT2

          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Uenaka, T. et al. Mol. Genet. 27 (22), 3974-3985, 2018)
          Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks Remember that you will be working with samples containing infectious virus.
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Uenaka, T. et al. Mol. Genet. 27 (22), 3974-3985, 2018)
          付加的提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。Venusの使用について、利用者は、提供承諾書を用いて、事前に理化学研究所脳神経科学研究センター・宮脇敦史博士の承諾を得る。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          宮脇敦史博士による提供承諾書 [Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16941 phRIT2 DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Uenaka, T., In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum. Mol. Genet. 27 (22): 3974-3985 (2018). PMID 30137437.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16941_A9Cqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: cPPT+CTS_v2_F
          Sequence file: RDB16941_A9Cqa.seq check
          >D06248A1_A9Cq_1_cPPT+CTS_v2_F_A01_01_ABI08.ab1
              1 GTTAGGCATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT
             61 TTATTACAGG GACAGCAGAA ATTCACTTTG AATTAATTCG ATTAATAGTA ATCAATTACG
            121 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
            181 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
            241 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
            301 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
            361 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT
            421 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC
            481 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC
            541 GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC
            601 TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA
            661 GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCCGCCACC ATGGAGGTAG AAAATGAAGC
            721 CAGCTGCTCC CCGGGCAGCG CATCAGGCGG GTCCAGAGAG TACAAGGTGG TAATGCTGGG
            781 AGCAGGGGGA GTTGGTAAAA GCGCAATGAC AATGCAGTTT ATTAGTCATC AGTTCCCTGA
            841 TTATCATGAC CCTACTATAG AAGATGCTTA TAAGACCCAG GTCAGGATTG ACAATGAGCC
            901 AGCTTACTTG GACATCTTGG ACACTGCTGG CCAGGCAGAA TTCACAGCCA TGCGGGAGCA
            961 GTACATGCGA GGTGGGAAGG CTTCATCATC TGCTACTCCG TCACTGACCG TCAATCATTT
           1021 CAGGAGGCTG CTAAGTTTAA GGAGCTCATT TTCCAGGTCG TCCACACTAA TGAAATCCCC
          1081 TGGATGCTGC TGGGCTTAAC CGAAAATTGA TTCCTGGGAA CACAG
          //
          Primer: IRES-R
          Sequence file: RDB16941_A9Cqb.seq check
          >D06248A1_A9Cq_1_IRES-R_B01_02_ABI08.ab1
              1 GATTTCGGGG GGCTTTTCGG CCCGTACCGG TTAGGGGGGG GGGGAGGGAG AGGGGCGGAT
             61 CCGCGGCCGC TCATGTCATA TTTTCTCTCT TCTTCTTCAA AGAACCTTTG AGCTTCTTCC
            121 ACAGGCTGTC TTTTCTCTTC AGTTTCTTTT CCATCAAGGA TGGCATGGAC TCCTTCTTGC
            181 GAATTTCCCT CACTAAGCCA TGAAAAGCAT CATCAATACA GAATCTGAGG GCTGCAGAGG
            241 TCTCAAAAAA ACCACAATTA TATTCTTGGG CAAGACTCAA GCCTTCTTCT GTAGAAACCT
            301 GGCGGAACTG TTCCAGATCA ATTTTGTTAC CCACCAGCAC CAGGGGAATT TCATAGGTGT
            361 GGCGGACCTG AAAAATGAGC TCTTTAAACT TGGCAGCCTC CTGAAATGAT TGACGGTCAG
            421 TGACGGAGTA GCAGATGATG AAGCCTTCCC CACCTCGCAT GTACTGCTCC CGCATGGCTG
            481 TGAATTCTGC CTGGCCAGCA GTGTCCAAGA TGTCCAAGTA AGCTGGCTCA TTGTCAATCC
            541 TGACCTGGGT CTTATAAGCA TCTTCTATAG TAGGGTCATG ATAATCAGGG AACTGATGAC
            601 TAATAAACTG CATTGTCATT GCGCTTTTAC CAACTCCCCC TGCTCCCAGC ATTACCACCT
            661 TGTACTCTCT GGACCCGCCT GATGCGCTGC CCGGGGAGCA GCTGGCTTCA TTTTCTACCT
            721 CCATGGTGGC GGCGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT TATATAGACC
            781 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT
            841 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGG
            901 ACTTGGAAAA TCCCGTGAGT CAAAACCGCT ATCCACGCCC ATTGATGTAC TGCCAAAACC
            961 GCAATCACCA ATGGGTAATA GCGATGACTA AATACGTAGA ATGTACTGGC CCAAGTAGGA
           1021 AGTTCCCATT AAGGTCATGT ACTTGGGGCA TAATGCAGCC GGCAATTTAA CCCGTCATTT
           1081 GACCGTCAAT AAGGGGGGCG GTACTTGGCA TATGAATACC CTTGGAATGT ACTTGCAAAG
          1141 TGGCCAGGTT TAACCGGATT A
          //
          Primer: HIVgp_R1
          Sequence file: RDB16941_A9Cqc.seq check
          >D06248A1_A9Cq_1_HIVgp_R1_C01_03_ABI08.ab1
              1 CCGGGCCTTC TATTCCCACT GCTCTTTTTT CTCTCTGCAC CACTCTTCTC TTTGCCTTGG
             61 TGGGTGCTAC TCCTAATGGT TCAATTTTTA CTACTTTATA TTTATATAAT TCACTTCTCC
            121 AATTGTCCCT CATATCTCCT CCTCCAGGTC TGAAGATCAG CGGCCGGCCG CTTGCTGTGC
            181 GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC TATCTTGTCT AAAGCTTCCT TGGTGTCTTT
            241 TATCTCTATC CTTTGATGCA CACAATAGAG GGTTGCTACT GTATTATATA ATGATCTAAG
            301 TTCTTCTGAT CCTGTCTGAA GGGATGGTTG TAGCTGTCCC AGTATTTGTC TACAGCCTTC
            361 TGATGTTTCT AACAGGCCAG GATTAACTGC GAATCGTTCT AGCTCCCTGC TTGCCCATAC
            421 TATATGTTTT AATTTATATT TTTTCTTTCC CCCTGGCCTT AACCGAATTT TTTCCCATCG
            481 CGATCTAATT CTCCCCCGCT TAATACTGAC GCTCTCGCAC CCATCTCTCT CCTTCTAGCC
            541 TCCGCTAGTC AAAATTTTTG GCGTACTCAC CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG
            601 CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA
            661 GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT
            721 CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG
            781 CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC
            841 TGGTCTAACC AGAGAGACCC AGTACAGGCA AAACGCGCTG CTTATATAGA CCTCCCACCG
            901 TACACGCCTA CCGCCCATTT GCGTCAATGG GGGCGGAGTT GTTACGACAT TTTGGGAAAG
            961 TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTTGG
           1021 AATCCCCGTG AGTCCAACCC GCTATCCACG CCCAATTGAT GTACTGCCGA AACGCATCAC
           1081 CATGGTAATA GCGAATGACT ATACGTTAGA TGTACCTGCC AAGGTAGGAA AGGTTCCCCA
          1141 ATTATAAA
          //
          Primer: IRES-F
          Sequence file: RDB16941_A9Cqd.seq check
          >D06248A1_A9Cq_1_IRES-F_D01_04_ABI08.ab1
              1 TGTTTCCTTT TCATGGTGTT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC
             61 GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACAACCA TGGTGAGCAA
            121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
            181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
            241 CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
            301 CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT
            361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
            421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
            481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
            541 CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC
            601 CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA
            661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA
            721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
            781 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAG AATTCAAGGC
            841 CTCTCGAGCC TCTAGAGTTA ACATCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT
            901 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TTACGCTATG
            961 TGGATACGCT GCTTTTAATG CCTTTGTATC ATGCTATTGC TTTCCCGTAT GGGCTTTCAT
           1021 TTTCTCCTCC TTGGTAATAA ATCCTGGTTG GCTGGTTCTT CTTTTATGAG GGAGGTGATG
           1081 GGCCGTTGTT CAGGCAACGT GCCGTTGGTG TTGCCACTGT GATTTGGCTT GAACGCCAAA
          1141 CTCTC
          //
          Primer: BGH_rev
          Sequence file: RDB16941_A9Cqe.seq check
          >D06248A1_A9Cq_1_BGH_rev_E01_05_ABI08.ab1
              1 GTTTAACGGG GGGCTTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
             61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
            121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
            181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA
            241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC
            301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT
            361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG
            421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA
            481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT
            541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG
            601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG
            661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG
            721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC
            781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT
            841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG
            901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC
            961 AGCAGCGGGG CAAGGCAGGC GGGCGATGAG TTTCCGGCCG TGGCAATAAG GGAGGGGGGA
           1021 AAGCGAAAGT CCCGGGAAAG GAGCTGAACA GTGGTGGGCA ATGCCCCATC AGTGGGGGGG
           1081 ATGCGTTCAG CCAAACACAG TGCCACACTA GTCACGTGCC TGGACAACGG GGCCACAACT
          1141 TCTTCAATTA GGAGAACTTG CCGAC
          //
          Primer: SV40pro_F
          Sequence file: RDB16941_A9Cqf.seq check
          >D06248A1_A9Cq_1_SV40pro_F_F01_06_ABI08.ab1
              1 CCTCGGGGCT TGGGGCTTTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
             61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
            121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
            181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
            241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
            301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
            361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
            421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
            481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
            541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
            601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
            661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
            721 ATAGCATCAC AAATTTCACA AATAAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGGTTT
            781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT
            841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
            901 CAAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG GTGCCCTAAT GAGTGAGCTA
            961 ACTCACATTA AATTGCGTTG GCGCTTCACT GGCCCCGCTT TTCCAGTCGG GGAAACCCTG
           1021 TCGTGCCAGC TGCATTTAAT GAAATCGGGC CAACGCGCCG GGAAGAGGCC GAATTTGGCG
           1081 GTATTGGGGC CGCTCTTCCG GCTTCGTCGG CTTCACTGGA CTCCGCTGCG CCTCGGGTTC
          1141 GATTTCAGAC CTGCGAGCGG AAGACGGGAT TATTCACGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Uenaka, T., In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum. Mol. Genet. 27 (22): 3974-3985 (2018). PMID 30137437.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl