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human TICAM-1 C Flag expression vector (#RDB16925)

Expression vector of human TICAM-1 tagged with FLAG at C-terminus.

Clone info. Expression vector of human Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule 1 (TICAM-1) tagged with FLAG at C-terminus.
Comment The full-length human TICAM-1 was cloned from mRNA of the human fibroblast cell line MRC-5. Expession was confirmed by the depositor.
Vector backbone pCMV-Flag (plasmid)
Selectable markers Amp^r
Gene/insert name human TICAM1 cDNA
Depositor|Developer Seya, Tsukasa |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Oshiumi, H. et al. Nat. Immunol. 4(2) : 161-167, 2003) and an acknowledgment to the DEPOSITOR are requested. Basic research use only. Do not pass the plasmids to the third persons without permission.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Oshiumi, H. et al. Nat. Immunol. 4(2) : 161-167, 2003) を引用し、謝辞の表明を必要とする。基礎研究に限る。許可なく第3者に渡すことを禁じる。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16925 human TICAM-1 C Flag expression vector DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TICAM-1 C Flag expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16925).

Reference section:

Oshiumi, H., Matsumoto, M., Funami, K., Akazawa, T., Seya, T., TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction. Nat. Immunol. 4 (2): 161-167 (2003). PMID 12539043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16925_A9B7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a_F2 ()
Region: EF-1a pro, insert 5'
Sequence file: RDB16925_A9B7a.seq check
>D06030A3_A9A7_2_EF1a_F2_A01_01_ABI08.ab1
    1 AGGGGTCTCT GGTGCTGGCC TCGCGCCGTC GTGTATCGCC CCGCCCTGGG CGGCAAGGCT
   61 GGTCTGGTCG GCACCAGTTG CGTGAGCGGA AAGATGGCCG CTTCCCGGCC CTGCTGCAGG
  121 GAGCTCAAAA TGGAGGACGC GGCGCTCGGG AGAGCGGGCG GGTGAGTCAC CCACACAAAG
  181 GAAAAGGGCC TTTCCGTCCT CAGCCGTCGC TTCATGTGAC TCCACGGAGT ACCGGGCGCC
  241 GTCCAGGCAC CTCGATTAGT TCTCGTGCTT TTGGAGTACG TCGTCTTTAG GTTGGGGGGA
  301 GGGGTTTTAT GCGATGGAGT TTCCCCACAC TGAGTGGGTG GAGACTGAAG TTAGGCCAGC
  361 TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT
  421 TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT
  481 TCTCTAGAGA TCCCTCGACC TCGAGTCACC ACCTCCAGCT TCTGCTGCCG GAGGCTGCAC
  541 CCACCTGTGC CCATGGCCTG CACGGGCCCA TCACTTCCTA GCGCCTTCGA CATTCTAGGT
  601 GCAGCAGGCC AGGACAAGCT CTTGTATCTG AAGCACAAAC TGAAGACCCC ACGCCCAGGC
  661 TGCCAGGGGC AGGACCTCCT GCATGCCATG GTTCTCCTGA AGCTGGGCCA GGAAACTGAG
  721 GCCAGGATCT CTCTAGAGGC ATTGAAGGCC GATGCGGTGG CCCGGCTGGT GGCCCGCCAG
  781 TGGGCTGGCG TGGACAGCAC CGAGGACCCA GAGGAGCCCC CAGATGTGTC CTGGGCTGTG
  841 GCCCGCTTGT ACCACCTGCT GGCTGAGGAG AAGCTGTGCC CCGCCTCGCT GCGGGACGTG
  901 GCCTACCAGG AAGCCGTCCG CACCCTCAGC TCCAGGGACG ACCACCGGCT GGGGGGAACT
  961 TCAAGATGAG GCCCGAAACC GGTGTGGGTG GGACATTGCT GGGGATCCAG GGAGCATCCG
 1021 GGACGCTCCA GTCCAATCTG GGCTGCCTCC CACCATCCTC GGGCTTTGCC CTCTGGGGAA
 1081 CAAGGAGCTC CCACGCCCCC ATTGACGGTG TTCGACCTGG AGCCTAGGAT GCTCCCTGCG
1141 AATTCCCACT GGGGCCAGGT CAT
//
Primer: hGCSF_R ()
Region: hGCSF 3'LTR + poly(A), FLAG, insert 3'
Sequence file: RDB16925_A9B7b.seq check
>D06030A3_A9A7_2_hGCSF_R_B01_02_ABI08.ab1
    1 GTGGGAAATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGTC GCGGCCGCTC
   61 ACTTGTCATC GTCGTCCTTG TAGTCTTCTG CCTCCTGCGT CTTGTCCTCG GGCGCCTGGG
  121 ACCCTCTCTG GTTCCACATG TGGTTGTTCA GCCCCAGCTG TACCATCTGT GCGTGGTGGA
  181 TAATGAGGGG TTGCAGCCCT GGGCTCTGAG GGGGTGCGGG TGAGGCCGTA GGGAAGGCTG
  241 GGGACTGCGG GAAGGGCAGT GACTGTGGAA AGGCTGCTGG CTGTGGGGAG GGCGGTGGGG
  301 GGGTGCCAGC CTGCCATGCG TGCAGGGGTG GCGGCTGCGG GCACCCCGGC CAAGTGGGAA
  361 AGGGTGGCGG GGCTCCCAGG GGCACCTGGC CCCCAAAGGG CATTCGAGCC CCATAGGGCG
  421 CCCCAGTCCC AAATGACATG TGGCTCCCAA AAGCCACCTG GAGCTGCTCC ATCTGTGCCT
  481 GGTAGGACAA GTAGCTCTGG AGGTAGGCTG AGTAGGCTGC GTTCAGTGCC GCCGCCTGCA
  541 TCCGCTCACC ATCCAGGTGT TGGCTCTGTT CCCGCAGGGC TCGGGTGTCC TGTTCCTTCC
  601 TCCACATGGC CTTTCGGGCC TGAAGCCTGT GGGGCTTGAA GGTGTTGGCC ACCTTCCTGG
  661 CGAAGATCTG GGAGTGTTCG TCCAGCCGCA CCAGCCCGGA GAGCAGGCTG GCCGTGTCGG
  721 AGCTGAGCTG GGCCGGGGAG CTCTCCAGGG GCAGGAAGGG GATGACACAG TCTGGCGACC
  781 CCTGTCGCGT GAGGTTGCTC ATCATGGCTT GGTTCACCTG GTGCAGGCTC AGGCGACAGT
  841 CGAAGTTGGA GGTGAGAAGT AGGATGATGA AAGCTGAGTG GTCTATGGCG TCCTGCAGGC
  901 AGCTCAGCTC CCCGCGCCCC GGCACCTGAA ATCCTCGCAG AAGGTGGCCC CGTCGGGCAC
  961 GCCAAAGGGC CCTCCAGCTT CTCCCGAACC CGCAGGCGAT GTGTTTCGTC TGCTCTGGCG
 1021 TGGAGGATCA CAAGTTTAAT AGAATTTCTG TTCCGATGAT GAATTTCCAG GGAGGGAAGG
 1081 AACAGGGAGA GGGGGTCAGG TGAGCCTGGA ACAGAGTAGA ATGAAGGAGG AAGGATGGTA
1141 ATGAAGGAGA GTAAGGGGGA TATG
//
Primer: pT2KXIG#1 ()
Region: SV40pro/ori, EF-1a pro
Sequence file: RDB16925_A9B7c.seq check
>D06030A3_A9A7_2_pT2KXIG#1_A01_01_ABI08.ab1
    1 GATTTAATTC CCTTCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA
   61 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT
  121 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC
  181 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
  241 CTTTGCAAAG ATGGATAAAG TTTTAAACAG AGAGGAATCT TTGCAGCTAA TGGACCTTCT
  301 AGGTCTTGAA AGGAGTGGGA ATTGGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC
  361 CCACAGTCCC CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACCGGTGCC TAGAGAAGGT
  421 GGCGCGGGGT AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG
  481 GGGGAGAACC GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG
  541 CCGCCAGAAC ACAGGTAAGT GCCGTGTGTG GTTCCCGCGG GCCTGGCCTC TTTACGGGTT
  601 ATGGCCCTTG CGTGCCTTGA ATTACTTCCA CCTGGCTGCA GTACGTGATT CTTGATCCCG
  661 AGCTTCGGGT TGGAAGTGGG TGGGAGAGTT CGAGGCCTTG CGCTTAAGGA GCCCCTTCGC
  721 CTCGTGCTTG AGTTGAGGCC TGGCCTGGGC GCTGGGGCCG CCGCGTGCGA ATCTGGTGGC
  781 ACCTTCGCGC CTGTCTCGCT GCTTTCGATA AGTCTCTAGC CATTTAAAAT TTTTGATGAC
  841 CTGCTGCGAC GCTTTTTTTC TGGCAAGATA GTCTTGTAAA TGCGGGCCAA GATCTGCACA
  901 CTGGTATTTC GGTTTTTGGG GCCGCGGGCG GCGACGGGGC CCGTGCGTCC CAGCGCACAT
  961 GTTCGGCGAG GCGGGGCCTG CGAGCGCGGC CACCGAGAAT CGGACGGGGT AGTCTCAAGC
 1021 TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC GCGTCGTGTA TCGCCCCGCC CTGGCGGCAA
 1081 GCTGTCTGGT CGCACCAGGT TTGCGTGAAC GGAAGATGGC TGCTTCCCTG TCCTGGCTGC
1141 AGGGGAGCTC CAAAAAATGG AAAGGTAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oshiumi, H., TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction. Nat. Immunol. 4 (2): 161-167 (2003). PMID 12539043. [link to RRC of NBRP]