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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

human TLR8-CFlag expression vector (#RDB16912)

Expression vector of human TLR8 tagged with FLAG at C-terminus.

Clone info. Expression vector of human Toll-like receptor 8 (TLR8) tagged with FLAG at C-terminus.
Comment Complementary DNAs for human TLR8 was cloned by RT-PCR from the mRNA of monocyte-derived immature DCs. Please look at AAZ95441 as a reference. Expression was confirmed by the depositor with immunoprecipitation and western blotting.
Vector backbone pEFBOS (plasmid)
Selectable markers Amp^r
Gene/insert name human TLR8 cDNA
Depositor|Developer Matsumoto, Misako |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014)。

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RDB16912 human TLR8-CFlag expression vector DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TLR8-CFlag expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16912).

Reference section:

Itoh, H., Tatematsu, M., Watanabe, A., Iwano, K., Funami, K., Seya, T., Matsumoto, M., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16912_A9Ajp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,insert 5'(TLR8 5')
Sequence file: RDB16912_A9Ajd.seq check
>D06031A4_A9Aj_2_EF1a-C_F_D05_11_ABI24.ab1
    1 NNNNNNNNNT NGNNNNTTTT NANTTTGGNN CTTGGTTCNT TCTCNAGCCT CAGACAGTGG
   61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGA TCCCTCGACC
  121 TCGAGGCCAC CATGAAGGAG TCATCTTTGC AAAATAGCTC CTGCAGCCTG GGAAAGGAGA
  181 CTAAAAAGGA AAACATGTTC CTTCAGTCGT CAATGCTGAC CTGCATTTTC CTGCTAATAT
  241 CTGGTTCCTG TGAGTTATGC GCCGAAGAAA ATTTTTCTAG AAGCTATCCT TGTGATGAGA
  301 AAAAGCAAAA TGACTCAGTT ATTGCAGAGT GCAGCAATCG TCGACTACAG GAAGTTCCCC
  361 AAACGGTGGG CAAATATGTG ACAGAACTAG ACCTGTCTGA TAATTTCATC ACACACATAA
  421 CGAATGAATC ATTTCAAGGG CTGCAAAATC TCACTAAAAT AAATCTAAAC CACAACCCCA
  481 ATGTACAGCA CCAGAACGGA AATCCCGGTA TACAATCAAA TGGCTTGAAT ATCACAGACG
  541 GGGCATTCCT CAACCTAAAA AACCTAAGGG AGTTACTGCT TGAAGACAAC CAGTTACCCC
  601 AAATACCCTC TGGTTTGCCA GAGTCTTTGA CAGAACTTAG TCTAATTCAA AACAATATAT
  661 ACAACATAAC TAAAGAGGGC ATTTCAAGAC TTATAAACTT GAAAAATCTC TATTTGGCCT
  721 GGAACTGCTA TTTTAACAAA GTTTGCGAGA AAACTAACAT AGAAGANGGA GTATTTGAAA
  781 CGCTGACAAA TTTGGAGTTG CTATCACTAT CTTTCAATTC TCTTTCACAC GTGCCACCNA
  841 AACTGCCAAG CTCCNTACGC AAACTTTTTC TGANCAACAC CCAGATCNAA TACNTTAGTG
  901 AAGAANANTN CNAGGGNNTG ATAAATTTNA CATTACNAGA NTTAAGCGGG AACTGTCCNA
  961 NGNGCTTCNN NGNCCNATTN NNNNGCGNGN CNTNGNNANG GNGGNGCTTC ANTTNATATA
1021 NATCGNTTTN GNTTTNNNAA ANNTNNNNCN NNNNTNNNNN NNNNN
//
Primer: hGCSF_R (Pr0031)
Region: insert 3'(FLAG,TLR8 3')
Sequence file: RDB16912_A9Aje.seq check
>D06031A4_A9Aj_2_hGCSF_R_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGTCNCNG GTGGGGNNCC TCACTCTNNG AGTCGCGGCC
   61 GCTTACTTGT CGTCATCGTC TTTGTAGTCG TATTGCTTAA TGGAATCGAC ATACATATTG
  121 TTATACCGTG AATCATTTTC AGTCAAGACC ACATTTCTCA GAGTTTGCCA AAACAAGCCT
  181 TCTGCCTTCG GGTTGTCAGG CCACTGGAGG ATGGAGCTCT TACAGATCCG CTGCCGTAGC
  241 CTCAAATACT GAGAATGCTG TAACACTGGC TCCAGCAGGA TAAATATAAT CACATCCATG
  301 TTCTCATCCA TTAGCCTCTG CAAAGCCAAG TAAAAAGCTG TTTTAAAGTT CCAGCTTTTT
  361 GCATATTTTT TGGTTAAAAC AAATACTGTT TTCTTGCTTT GGTTGATGCT CTGCATGAGG
  421 TTGTCGATGA TGGCCAATCC CGGGTCCCAA TCCCTCTCCT CTAGACAAAG GAGAACGTTT
  481 TTGTCTCGGC TCTCTTCAAG GTGGTAGCGC AGCTCATTTA TCACCCAGTC AGTAACAGAG
  541 GCATCTTTGG TGTCATAAGA AATGTAAGCA TCATAGAAAG TTTGGGATGT GGAAAGAGAC
  601 CTGTAGCCTT TTACCTTAGC TAAACACACA TTATATATAA ACCAAACATC CCAGTAAAAC
  661 AAATGGTGAG CCAGGGCAGC CAACATAACC ATGGTGGTGA TAAAGAACGT GAAGAAAAAT
  721 AATATCACTG CAGTGACATC TGAAACACAA GTTGTTAGCT CCNGACTCAC AATACTCTTC
  781 CCTCTTTGAT CCCCAGGANT GGCACAAATG ACATCTACCA GTCTGGGAAT TTTGACATTC
  841 AGATGTTCAT CCNTCCATCT TCGGAAATCT CCAATGTCAC AGGNGCATTN NAAGGGGTTT
  901 CCGNGTANTT NCNACATAGA TAATTTGGTG GNGGTCTTAN TTTCAAGTGN GGNNTTGNTT
  961 ANNGNTTTTA NCNNANTGGN ANNNNNAATN NANGTGNTTC NNANNACTGA CTTCNNAAAN
1021 AAANNNNNNN GGNAGGTGGN NAANNNNGNN ATGANNNNNN NNNN
//
Primer: reverse (Pr0099)
Region: SV40 pro_ori,EF-1 alpha pro
Sequence file: RDB16912_A9Ajf.seq check
>D06031A4_A9Aj_2_reverse_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAG GCAGAAGTNN GCNAAGCATG CATCTCNATT AGTCNGCAAC
   61 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC
  121 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC
  181 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT
  241 TTGCAAAGAT GGATAAAGTT TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG
  301 GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC
  361 ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG
  421 CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG
  481 GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC
  541 GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT
  601 GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT ACGTGATTCT TGATCCCGAG
  661 CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT
  721 CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC
  781 CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA TTTAAAATTT TTGATGACCT
  841 GCTGCGACGC TNTTTTTCTG GNAAGATAGT CTTGTAAATG CGNGCCNAGA TCTGNACACN
  901 GGNATTTCGG TTTTTGGGGN CGCGGGCNNC GANNGGGCCN GTGCGNCCCN NCGCNCNTGN
  961 TCGGNGANNN GGGGNNNGCN ANNNNNGNNN NNNNANAANN NNNNNGGGGT ANNNNCNNNC
1021 NNGNNNNGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, H., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301. [link to RRC of NBRP]