Sequencing and PCR primers for primer information.
Expression vector of human TLR8 tagged with FLAG at C-terminus.
| Catalog number | RDB16912 |
|---|---|
| Resource name | human TLR8-CFlag expression vector |
| Clone info. | Expression vector of human Toll-like receptor 8 (TLR8) tagged with FLAG at C-terminus. Complementary DNAs for human TLR8 was cloned by RT-PCR from the mRNA of monocyte-derived immature DCs. Please look at AAZ95441 as a reference. Expression was confirmed by the depositor with immunoprecipitation and western blotting. |
| Vector backbone | pEFBOS (plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | human TLR8 cDNA |
| Depositor | Matsumoto, Misako | |
| Other clones in our bank | human TLR8 (NCBI Gene 51311) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014) |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16912 | human TLR8-CFlag expression vector | DNA solution |
Electronic file
Original reference
| original | Itoh, H., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301. |
|---|
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16912_A9Ajp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Region: EF1alpha pro,insert5' | Primer: EF1a-C_F | Sequence: RDB16912_A9Aja.seq |
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>D06031A4_A9Aj_2_EF1a-C_F_D05_11_ABI24.ab1 1 TCATGGAATT TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG 61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGA TCCCTCGACC 121 TCGAGGCCAC CATGAAGGAG TCATCTTTGC AAAATAGCTC CTGCAGCCTG GGAAAGGAGA 181 CTAAAAAGGA AAACATGTTC CTTCAGTCGT CAATGCTGAC CTGCATTTTC CTGCTAATAT 241 CTGGTTCCTG TGAGTTATGC GCCGAAGAAA ATTTTTCTAG AAGCTATCCT TGTGATGAGA 301 AAAAGCAAAA TGACTCAGTT ATTGCAGAGT GCAGCAATCG TCGACTACAG GAAGTTCCCC 361 AAACGGTGGG CAAATATGTG ACAGAACTAG ACCTGTCTGA TAATTTCATC ACACACATAA 421 CGAATGAATC ATTTCAAGGG CTGCAAAATC TCACTAAAAT AAATCTAAAC CACAACCCCA 481 ATGTACAGCA CCAGAACGGA AATCCCGGTA TACAATCAAA TGGCTTGAAT ATCACAGACG 541 GGGCATTCCT CAACCTAAAA AACCTAAGGG AGTTACTGCT TGAAGACAAC CAGTTACCCC 601 AAATACCCTC TGGTTTGCCA GAGTCTTTGA CAGAACTTAG TCTAATTCAA AACAATATAT 661 ACAACATAAC TAAAGAGGGC ATTTCAAGAC TTATAAACTT GAAAAATCTC TATTTGGCCT 721 GGAACTGCTA TTTTAACAAA GTTTGCGAGA AAACTAACAT AGAAGATGGA GTATTTGAAA 781 CGCTGACAAA TTTGGAGTTG CTATCACTAT CTTTCAATTC TCTTTCACAC GTGCCACCCA 841 AACTGCCAAG CTCCCTACGC AAACTTTTTC TGAGCAACAC CCAGATCAAA TACATTAGTG 901 AAGAAGATTT CAAGGGATTG ATAAATTTAA CATTACTAGA TTTAAGCGGG AACTGTCCGA 961 GGTGCTTCAA TGCCCCATTT CCATGCGTGC CCTTGTGATG GTGGTGCTTC AATTAATATA 1021 GATCGTTTTT GCTTTTCAAA ACTTTGACCC AACTTTCGAT ACCTAAACCT CTCTAGCACT 1081 TTCCCCTCAA GAAGATATGC TGCCCTGGAT TTTAAAAATA TGGCTTCAAT CTGAGGTGCC 1141 TGGATCTTGA ATTCAACTAT TTTAAGGTGG GGAAGAAAAA ATATAGAC // |
| Region: hGCSF p(A),FLAG,insert3' | Primer: hGCSF_R | Sequence: RDB16912_A9Ajb.seq |
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>D06031A4_A9Aj_2_hGCSF_R_E05_14_ABI24.ab1 1 TGGGGGAACT GAAACTCTCA AGGGTCCCAG GTGGGGACCC TCACTCTAAG AGTCGCGGCC 61 GCTTACTTGT CGTCATCGTC TTTGTAGTCG TATTGCTTAA TGGAATCGAC ATACATATTG 121 TTATACCGTG AATCATTTTC AGTCAAGACC ACATTTCTCA GAGTTTGCCA AAACAAGCCT 181 TCTGCCTTCG GGTTGTCAGG CCACTGGAGG ATGGAGCTCT TACAGATCCG CTGCCGTAGC 241 CTCAAATACT GAGAATGCTG TAACACTGGC TCCAGCAGGA TAAATATAAT CACATCCATG 301 TTCTCATCCA TTAGCCTCTG CAAAGCCAAG TAAAAAGCTG TTTTAAAGTT CCAGCTTTTT 361 GCATATTTTT TGGTTAAAAC AAATACTGTT TTCTTGCTTT GGTTGATGCT CTGCATGAGG 421 TTGTCGATGA TGGCCAATCC CGGGTCCCAA TCCCTCTCCT CTAGACAAAG GAGAACGTTT 481 TTGTCTCGGC TCTCTTCAAG GTGGTAGCGC AGCTCATTTA TCACCCAGTC AGTAACAGAG 541 GCATCTTTGG TGTCATAAGA AATGTAAGCA TCATAGAAAG TTTGGGATGT GGAAAGAGAC 601 CTGTAGCCTT TTACCTTAGC TAAACACACA TTATATATAA ACCAAACATC CCAGTAAAAC 661 AAATGGTGAG CCAGGGCAGC CAACATAACC ATGGTGGTGA TAAAGAACGT GAAGAAAAAT 721 AATATCACTG CAGTGACATC TGAAACACAA GTTGTTAGCT CCAGACTCAC AATACTCTTC 781 CCTCTTTGAT CCCCAGGACT GGCACAAATG ACATCTACCA GTCTGGGAAT TTTGACATTC 841 AGATGTTCAT CCATCCATCT TCGGAAATCT CCAATGTCAC AGGTGCATTC AAAGGGGTTT 901 CCGTGTAGTT CCAACATAGA TAATTTGGTG GTGGTCTTAG TTTCAAGTGC GGATTTGTTT 961 ATTGTTTTTA GCAGATTGGA AACTTAAATC GAGGTGCTTC AGACTACTGA CTTCAGAAAG 1021 AAAGCCAGAG GGTAGGTGGG AAATCCTGTT ATGACTCAGC AGCAGTGTCC GAAGGAAGAT 1081 GTAAAGTCAG ATAGGGCTAT CAGGTTAAAA GAGTAGGTTT GTTTCCACGT AGTCAAGCAC 1141 TCGAGACGAG AACCTGCCTG AAGTAATGTC CAGGTTAAAA AACCTTTTAA CACATA // |
| Region: SV40pro_ori,EF1alpha pro | Primer: reverse | Sequence: RDB16912_A9Ajc.seq |
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>D06031A4_A9Aj_2_reverse_F05_17_ABI24.ab1 1 CTAAATTTCC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 61 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC 121 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC 181 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT 241 TTGCAAAGAT GGATAAAGTT TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG 301 GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC 361 ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG 421 CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG 481 GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC 541 GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT 601 GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT ACGTGATTCT TGATCCCGAG 661 CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT 721 CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC 781 CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA TTTAAAATTT TTGATGACCT 841 GCTGCGACGC TTTTTTTCTG GCAAGATAGT CTTGTAAATG CGGGCCAAGA TCTGCACACT 901 GGTATTTCGG TTTTTGGGGC CGCGGGCGGC GACGGGGCCC GTGCGTCCCA GCGCACATGT 961 TCGGCGAGGC GGGGCCTGCG AGCGCGGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC 1021 TGGGCCGGGC CTGCTCTGGG TGCCTGGCCT CGCGCCCGTC GTGTATCGCC CCCGCCTGGC 1081 GGCAAGGCTT GGTCTGGTCG GCACCAGTTG CGGTGGAGCG AAAGATGACG CATCCTGATC 1141 CTTGCTGCCA GGGAGCTCAA AATTGAGGGA CGCGCGGCGG CCTCTCCG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Itoh, H., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301. |
|---|
2019.09.10
GNP_filter3_RDBDEP_html_190816.pl