Resource data sheet
Prev.

human TLR8-CFlag expression vector

Expression vector of human TLR8 tagged with FLAG at C-terminus.

Catalog number RDB16912
Resource name human TLR8-CFlag expression vector
Clone info. Expression vector of human Toll-like receptor 8 (TLR8) tagged with FLAG at C-terminus.
Complementary DNAs for human TLR8 was cloned by RT-PCR from the mRNA of monocyte-derived immature DCs. Please look at AAZ95441 as a reference. Expression was confirmed by the depositor with immunoprecipitation and western blotting.
Vector backbone pEFBOS (plasmid)
Selectable markers Amp^r
Gene/insert name human TLR8 cDNA
Depositor Matsumoto, Misako |
 
Other clones in our bank human TLR8 (NCBI Gene 51311) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014)
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RDB16912 human TLR8-CFlag expression vector DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Itoh, H., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16912_A9Ajp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: EF1alpha pro,insert5' | Primer: EF1a-C_F | Sequence: RDB16912_A9Aja.seq
>D06031A4_A9Aj_2_EF1a-C_F_D05_11_ABI24.ab1
    1 TCATGGAATT TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG
   61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGA TCCCTCGACC
  121 TCGAGGCCAC CATGAAGGAG TCATCTTTGC AAAATAGCTC CTGCAGCCTG GGAAAGGAGA
  181 CTAAAAAGGA AAACATGTTC CTTCAGTCGT CAATGCTGAC CTGCATTTTC CTGCTAATAT
  241 CTGGTTCCTG TGAGTTATGC GCCGAAGAAA ATTTTTCTAG AAGCTATCCT TGTGATGAGA
  301 AAAAGCAAAA TGACTCAGTT ATTGCAGAGT GCAGCAATCG TCGACTACAG GAAGTTCCCC
  361 AAACGGTGGG CAAATATGTG ACAGAACTAG ACCTGTCTGA TAATTTCATC ACACACATAA
  421 CGAATGAATC ATTTCAAGGG CTGCAAAATC TCACTAAAAT AAATCTAAAC CACAACCCCA
  481 ATGTACAGCA CCAGAACGGA AATCCCGGTA TACAATCAAA TGGCTTGAAT ATCACAGACG
  541 GGGCATTCCT CAACCTAAAA AACCTAAGGG AGTTACTGCT TGAAGACAAC CAGTTACCCC
  601 AAATACCCTC TGGTTTGCCA GAGTCTTTGA CAGAACTTAG TCTAATTCAA AACAATATAT
  661 ACAACATAAC TAAAGAGGGC ATTTCAAGAC TTATAAACTT GAAAAATCTC TATTTGGCCT
  721 GGAACTGCTA TTTTAACAAA GTTTGCGAGA AAACTAACAT AGAAGATGGA GTATTTGAAA
  781 CGCTGACAAA TTTGGAGTTG CTATCACTAT CTTTCAATTC TCTTTCACAC GTGCCACCCA
  841 AACTGCCAAG CTCCCTACGC AAACTTTTTC TGAGCAACAC CCAGATCAAA TACATTAGTG
  901 AAGAAGATTT CAAGGGATTG ATAAATTTAA CATTACTAGA TTTAAGCGGG AACTGTCCGA
  961 GGTGCTTCAA TGCCCCATTT CCATGCGTGC CCTTGTGATG GTGGTGCTTC AATTAATATA
 1021 GATCGTTTTT GCTTTTCAAA ACTTTGACCC AACTTTCGAT ACCTAAACCT CTCTAGCACT
 1081 TTCCCCTCAA GAAGATATGC TGCCCTGGAT TTTAAAAATA TGGCTTCAAT CTGAGGTGCC
1141 TGGATCTTGA ATTCAACTAT TTTAAGGTGG GGAAGAAAAA ATATAGAC
//
Region: hGCSF p(A),FLAG,insert3' | Primer: hGCSF_R | Sequence: RDB16912_A9Ajb.seq
>D06031A4_A9Aj_2_hGCSF_R_E05_14_ABI24.ab1
    1 TGGGGGAACT GAAACTCTCA AGGGTCCCAG GTGGGGACCC TCACTCTAAG AGTCGCGGCC
   61 GCTTACTTGT CGTCATCGTC TTTGTAGTCG TATTGCTTAA TGGAATCGAC ATACATATTG
  121 TTATACCGTG AATCATTTTC AGTCAAGACC ACATTTCTCA GAGTTTGCCA AAACAAGCCT
  181 TCTGCCTTCG GGTTGTCAGG CCACTGGAGG ATGGAGCTCT TACAGATCCG CTGCCGTAGC
  241 CTCAAATACT GAGAATGCTG TAACACTGGC TCCAGCAGGA TAAATATAAT CACATCCATG
  301 TTCTCATCCA TTAGCCTCTG CAAAGCCAAG TAAAAAGCTG TTTTAAAGTT CCAGCTTTTT
  361 GCATATTTTT TGGTTAAAAC AAATACTGTT TTCTTGCTTT GGTTGATGCT CTGCATGAGG
  421 TTGTCGATGA TGGCCAATCC CGGGTCCCAA TCCCTCTCCT CTAGACAAAG GAGAACGTTT
  481 TTGTCTCGGC TCTCTTCAAG GTGGTAGCGC AGCTCATTTA TCACCCAGTC AGTAACAGAG
  541 GCATCTTTGG TGTCATAAGA AATGTAAGCA TCATAGAAAG TTTGGGATGT GGAAAGAGAC
  601 CTGTAGCCTT TTACCTTAGC TAAACACACA TTATATATAA ACCAAACATC CCAGTAAAAC
  661 AAATGGTGAG CCAGGGCAGC CAACATAACC ATGGTGGTGA TAAAGAACGT GAAGAAAAAT
  721 AATATCACTG CAGTGACATC TGAAACACAA GTTGTTAGCT CCAGACTCAC AATACTCTTC
  781 CCTCTTTGAT CCCCAGGACT GGCACAAATG ACATCTACCA GTCTGGGAAT TTTGACATTC
  841 AGATGTTCAT CCATCCATCT TCGGAAATCT CCAATGTCAC AGGTGCATTC AAAGGGGTTT
  901 CCGTGTAGTT CCAACATAGA TAATTTGGTG GTGGTCTTAG TTTCAAGTGC GGATTTGTTT
  961 ATTGTTTTTA GCAGATTGGA AACTTAAATC GAGGTGCTTC AGACTACTGA CTTCAGAAAG
 1021 AAAGCCAGAG GGTAGGTGGG AAATCCTGTT ATGACTCAGC AGCAGTGTCC GAAGGAAGAT
 1081 GTAAAGTCAG ATAGGGCTAT CAGGTTAAAA GAGTAGGTTT GTTTCCACGT AGTCAAGCAC
1141 TCGAGACGAG AACCTGCCTG AAGTAATGTC CAGGTTAAAA AACCTTTTAA CACATA
//
Region: SV40pro_ori,EF1alpha pro | Primer: reverse | Sequence: RDB16912_A9Ajc.seq
>D06031A4_A9Aj_2_reverse_F05_17_ABI24.ab1
    1 CTAAATTTCC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC
   61 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC
  121 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC
  181 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT
  241 TTGCAAAGAT GGATAAAGTT TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG
  301 GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC
  361 ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG
  421 CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG
  481 GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC
  541 GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT
  601 GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT ACGTGATTCT TGATCCCGAG
  661 CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT
  721 CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC
  781 CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA TTTAAAATTT TTGATGACCT
  841 GCTGCGACGC TTTTTTTCTG GCAAGATAGT CTTGTAAATG CGGGCCAAGA TCTGCACACT
  901 GGTATTTCGG TTTTTGGGGC CGCGGGCGGC GACGGGGCCC GTGCGTCCCA GCGCACATGT
  961 TCGGCGAGGC GGGGCCTGCG AGCGCGGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC
 1021 TGGGCCGGGC CTGCTCTGGG TGCCTGGCCT CGCGCCCGTC GTGTATCGCC CCCGCCTGGC
 1081 GGCAAGGCTT GGTCTGGTCG GCACCAGTTG CGGTGGAGCG AAAGATGACG CATCCTGATC
1141 CTTGCTGCCA GGGAGCTCAA AATTGAGGGA CGCGCGGCGG CCTCTCCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, H., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301.

2019.09.10

GNP_filter3_RDBDEP_html_190816.pl