icon below as well as clone information before placing your order.
Expression vector of human TLR8 tagged with FLAG at C-terminus.
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External Database human TLR8 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014) |
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| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014)。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB16912 | human TLR8-CFlag expression vector | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The human TLR8-CFlag expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16912). |
Reference section:
| Itoh, H., Tatematsu, M., Watanabe, A., Iwano, K., Funami, K., Seya, T., Matsumoto, M., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB16912_A9Ajp1-3.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(TLR8 5') Sequence file: RDB16912_A9Ajd.seq
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>D06031A4_A9Aj_2_EF1a-C_F_D05_11_ABI24.ab1 1 NNNNNNNNNT NGNNNNTTTT NANTTTGGNN CTTGGTTCNT TCTCNAGCCT CAGACAGTGG 61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGA TCCCTCGACC 121 TCGAGGCCAC CATGAAGGAG TCATCTTTGC AAAATAGCTC CTGCAGCCTG GGAAAGGAGA 181 CTAAAAAGGA AAACATGTTC CTTCAGTCGT CAATGCTGAC CTGCATTTTC CTGCTAATAT 241 CTGGTTCCTG TGAGTTATGC GCCGAAGAAA ATTTTTCTAG AAGCTATCCT TGTGATGAGA 301 AAAAGCAAAA TGACTCAGTT ATTGCAGAGT GCAGCAATCG TCGACTACAG GAAGTTCCCC 361 AAACGGTGGG CAAATATGTG ACAGAACTAG ACCTGTCTGA TAATTTCATC ACACACATAA 421 CGAATGAATC ATTTCAAGGG CTGCAAAATC TCACTAAAAT AAATCTAAAC CACAACCCCA 481 ATGTACAGCA CCAGAACGGA AATCCCGGTA TACAATCAAA TGGCTTGAAT ATCACAGACG 541 GGGCATTCCT CAACCTAAAA AACCTAAGGG AGTTACTGCT TGAAGACAAC CAGTTACCCC 601 AAATACCCTC TGGTTTGCCA GAGTCTTTGA CAGAACTTAG TCTAATTCAA AACAATATAT 661 ACAACATAAC TAAAGAGGGC ATTTCAAGAC TTATAAACTT GAAAAATCTC TATTTGGCCT 721 GGAACTGCTA TTTTAACAAA GTTTGCGAGA AAACTAACAT AGAAGANGGA GTATTTGAAA 781 CGCTGACAAA TTTGGAGTTG CTATCACTAT CTTTCAATTC TCTTTCACAC GTGCCACCNA 841 AACTGCCAAG CTCCNTACGC AAACTTTTTC TGANCAACAC CCAGATCNAA TACNTTAGTG 901 AAGAANANTN CNAGGGNNTG ATAAATTTNA CATTACNAGA NTTAAGCGGG AACTGTCCNA 961 NGNGCTTCNN NGNCCNATTN NNNNGCGNGN CNTNGNNANG GNGGNGCTTC ANTTNATATA 1021 NATCGNTTTN GNTTTNNNAA ANNTNNNNCN NNNNTNNNNN NNNNN // |
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Primer: hGCSF_R (Pr0031) Region: insert 3'(FLAG,TLR8 3') Sequence file: RDB16912_A9Aje.seq
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>D06031A4_A9Aj_2_hGCSF_R_E05_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGTCNCNG GTGGGGNNCC TCACTCTNNG AGTCGCGGCC 61 GCTTACTTGT CGTCATCGTC TTTGTAGTCG TATTGCTTAA TGGAATCGAC ATACATATTG 121 TTATACCGTG AATCATTTTC AGTCAAGACC ACATTTCTCA GAGTTTGCCA AAACAAGCCT 181 TCTGCCTTCG GGTTGTCAGG CCACTGGAGG ATGGAGCTCT TACAGATCCG CTGCCGTAGC 241 CTCAAATACT GAGAATGCTG TAACACTGGC TCCAGCAGGA TAAATATAAT CACATCCATG 301 TTCTCATCCA TTAGCCTCTG CAAAGCCAAG TAAAAAGCTG TTTTAAAGTT CCAGCTTTTT 361 GCATATTTTT TGGTTAAAAC AAATACTGTT TTCTTGCTTT GGTTGATGCT CTGCATGAGG 421 TTGTCGATGA TGGCCAATCC CGGGTCCCAA TCCCTCTCCT CTAGACAAAG GAGAACGTTT 481 TTGTCTCGGC TCTCTTCAAG GTGGTAGCGC AGCTCATTTA TCACCCAGTC AGTAACAGAG 541 GCATCTTTGG TGTCATAAGA AATGTAAGCA TCATAGAAAG TTTGGGATGT GGAAAGAGAC 601 CTGTAGCCTT TTACCTTAGC TAAACACACA TTATATATAA ACCAAACATC CCAGTAAAAC 661 AAATGGTGAG CCAGGGCAGC CAACATAACC ATGGTGGTGA TAAAGAACGT GAAGAAAAAT 721 AATATCACTG CAGTGACATC TGAAACACAA GTTGTTAGCT CCNGACTCAC AATACTCTTC 781 CCTCTTTGAT CCCCAGGANT GGCACAAATG ACATCTACCA GTCTGGGAAT TTTGACATTC 841 AGATGTTCAT CCNTCCATCT TCGGAAATCT CCAATGTCAC AGGNGCATTN NAAGGGGTTT 901 CCGNGTANTT NCNACATAGA TAATTTGGTG GNGGTCTTAN TTTCAAGTGN GGNNTTGNTT 961 ANNGNTTTTA NCNNANTGGN ANNNNNAATN NANGTGNTTC NNANNACTGA CTTCNNAAAN 1021 AAANNNNNNN GGNAGGTGGN NAANNNNGNN ATGANNNNNN NNNN // |
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Primer: reverse (Pr0099) Region: SV40 pro_ori,EF-1 alpha pro Sequence file: RDB16912_A9Ajf.seq
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>D06031A4_A9Aj_2_reverse_F05_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNAG GCAGAAGTNN GCNAAGCATG CATCTCNATT AGTCNGCAAC 61 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC 121 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC 181 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT 241 TTGCAAAGAT GGATAAAGTT TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG 301 GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC 361 ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG 421 CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG 481 GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC 541 GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT 601 GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT ACGTGATTCT TGATCCCGAG 661 CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT 721 CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC 781 CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA TTTAAAATTT TTGATGACCT 841 GCTGCGACGC TNTTTTTCTG GNAAGATAGT CTTGTAAATG CGNGCCNAGA TCTGNACACN 901 GGNATTTCGG TTTTTGGGGN CGCGGGCNNC GANNGGGCCN GTGCGNCCCN NCGCNCNTGN 961 TCGGNGANNN GGGGNNNGCN ANNNNNGNNN NNNNANAANN NNNNNGGGGT ANNNNCNNNC 1021 NNGNNNNGNN NNNNNNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Itoh, H., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301. [link to RRC of NBRP] |
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2023.08.15
GNP_filter3_RDBDEP_html_230702.pl