Resource data sheet
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human TLR3-CFlag expression vector

Expression vector of human TLR3 tagged with FLAG at C-terminus.

Catalog number RDB16911
Resource name human TLR3-CFlag expression vector
Clone info. Expression vector of human Toll-like receptor 3 (TLR3) tagged with FLAG at C-terminus.
Complementary DNAs for human TLR3 was cloned by RT-PCR from the mRNA of monocyte-derived immature DCs. Expression was confirmed by the depositor with immunoprecipitation and western blotting.
Vector backbone pEFBOS (plasmid)
Selectable markers Amp^r
Gene/insert name human TLR3 cDNA
Depositor Matsumoto, Misako |
 
Other clones in our bank human TLR3 (NCBI Gene 7098) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)
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RDB16911 human TLR3-CFlag expression vector DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Matsumoto, M., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16911_A9Ajp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: EF1alpha pro,insert5' | Primer: EF1a-C_F | Sequence: RDB16911_A9Aja.seq
>D06031A3_A9Aj_2_EF1a-C_F_A05_02_ABI24.ab1
    1 TCATGGGATG CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA
  121 GGCCGCCACC ATGAGACAGA CTTTGCCTTG TATCTACTTT TGGGGGGGCC TTTTGCCCTT
  181 TGGGATGCTG TGTGCATCCT CCACCACCAA GTGCACTGTT AGCCATGAAG TTGCTGACTG
  241 CAGCCACCTG AAGTTGACTC AGGTACCCGA TGATCTACCC ACAAACATAA CAGTGTTGAA
  301 CCTTACCCAT AATCAACTCA GAAGATTACC AGCCGCCAAC TTCACAAGGT ATAGCCAGCT
  361 AACTAGCTTG GATGTAGGAT TTAACACCAT CTCAAAACTG GAGCCAGAAT TGTGCCAGAA
  421 ACTTCCCATG TTAAAAGTTT TGAACCTCCA GCACAATGAG CTATCTCAAC TTTCTGATAA
  481 AACCTTTGCC TTCTGCACGA ATTTGACTGA ACTCCATCTC ATGTCCAACT CAATCCAGAA
  541 AATTAAAAAT AATCCCTTTG TCAAGCAGAA GAATTTAATC ACATTAGATC TGTCTCATAA
  601 TGGCTTGTCA TCTACAAAAT TAGGAACTCA GGTTCAGCTG GAAAATCTCC AAGAGCTTCT
  661 ATTATCAAAC AATAAAATTC AAGCGCTAAA AAGTGAAGAA CTGGATATCT TTGCCAATTC
  721 ATCTTTAAAA AAATTAGAGT TGTCATCGAA TCAAATTAAA GAGTTTTCTC CAGGGTGTTT
  781 TCACGCAATT GGAAGATTAT TTGGCCTCTT TCTGAACAAT GTCCAGCTGG GTCCCAGCCT
  841 TACAGAGAAG CTATGTTTGG AATTAGCAAA CACAAGCATT CGGAATCTGT CTCTGAGTAA
  901 CAGCCAGCTG TCCACCACCA GCAATACAAC TTTCTTGGGA CTAAAGTGGA CAAATCTCAC
  961 TATGCTCGAT CTTTCCTACA ACAACTTAAA TGTGGTTGGT AACGATTCCT TTGCTTGGCT
 1021 TCCACAACTA GAATATTTTC TTCCCTAGAG TATAATAATA TACAGCATTT GTTTTTCTCA
 1081 CTCTTTTGCA CGGCTTTTCA TGGTGAAGGT ACCCTGATTT GGAACCGCTC TTTTAACCTA
1141 AACCAAGTAT TTCCCTTGCT TCACTTCTTA AGGAATTTGA ATGGT
//
Region: hGCSF p(A),FLAG,insert3' | Primer: hGCSF_R | Sequence: RDB16911_A9Ajb.seq
>D06031A3_A9Aj_2_hGCSF_R_B05_05_ABI24.ab1
    1 GGGGGGGAAT TATCTCTCAG GGTCCCAGGT GGGGACCCTC ACTCTAAGAG TCGCGGCCGC
   61 TTACTTGTCA TCGTCGTCCT TGTAGTCATG TACAGAGTTT TTGGATCCAA GTGCTACTTG
  121 CAATTTATGG CGAAAGGCAC CTATCCGTTC TTTCTGAACT GGCCAGTTCA AGATGCAGTG
  181 AGATTTAAAC ATTCCTCTTC GCAAACAGAG TGCATGGTTC AGTTTATAAT CTGGAATCTC
  241 CTCAAGGAAA ACCAATATAA TGGAATCCAG ATTTTGTTCA ATAGCTTGTT GAACTGCATG
  301 ATGTACCTTG AATCTTTTGC ATAATGGGTC TTTTAATAGA TGGTGTGTTA TAACAAAAAT
  361 AATTTTTCTG CTTCTTTTGA TGCTGTTAAC AATTGCTTCT AGTTCAAAAA CACCCGCCTC
  421 AAAGTCCCTT TCTTCCAGAC AAAATTTGAG AGATTGGTCT TCCTTTTCCA TTGAAGAGAA
  481 ATGTTCCCAG ACCCAATCCT TATCTTTATA GGCATGAATT ATATATGCTG CATATTCAAA
  541 CTGTTCTGTC TGTCTGTCTA TTTCTTTGAA ACCAAGAACT CGATGTACTG AAACATTCCA
  601 ATAAAAAGAT ATCCTCCAGC CCTCAAAGTG GATGAGAAGT ACAATAAAGA TAAAAATCAA
  661 CAGGATACTG GTATTGATCA TGAAAAAGAG TTCAAAGGGG GCACTGTCTT TGCAAGATGA
  721 TGTATCAAAA AGTCTCACTG GGAACCCATG ATAGTGAGGT GGAGTGTTGC AAAGGTAGTG
  781 GCTTGACAGC TCAGGGATGT TGGTATGGGT CTCGTTAATC CAATTAACAA ACCAGGCAAT
  841 ACTTTCACAC GTGCAATCAA AGGGATTAAA GCGCATATCT AACTCAGTCA GGTTCCTGAA
  901 AGCTGGCCCG AAAACCTTCT TCTCAACGGA TGTTATGAGA TTCTTCTGAA GGTTCAATGA
  961 CTTTAGAGAC ACCTGATTAT TAAAGACAGA TGCTGGAAGT GTGTTTAAAT TTATTCAATC
 1021 CTAAATCGAT GATCTTTAGT TCAATAAATC CCTTGAAGAC CTTCAACTGG GATCTCGTCA
 1081 AAGCCGTTGG GACTCCAAGT TAGGATGGTG AGTGAGAACA GACCCTTTAG GAATTATTGG
1141 GACACATGTT TGCGTGTTTC AGAGACGTGC TAAGGTTGTG TTTATGGCTG C
//
Region: SV40pro_ori,EF1alpha pro | Primer: reverse | Sequence: RDB16911_A9Ajc.seq
>D06031A3_A9Aj_2_reverse_C05_08_ABI24.ab1
    1 CATATTCCTC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT
   61 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC
  121 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA
  181 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTTG
  241 CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA GCTAATGGAC CTTCTAGGTC
  301 TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA
  361 GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC
  421 GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA
  481 GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC
  541 AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC
  601 CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT
  661 CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT
  721 GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT
  781 CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT AAAATTTTTG ATGACCTGCT
  841 GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG GCCAAGATCT GCACACTGGT
  901 ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG CACATGTTCG
  961 GCGAGGCGGG GCCTGCGAGC GCGGCCACCG AGAATCGGAC GGGGGTAGTC TCAAGCTGGG
 1021 CCGGGCCTGC TCTGGGTGCC TGGCCTCGCG CCGTCGTGTA TCGCCCCGCC CTGGCGGCAA
 1081 GGCTGGTCTG TCGGCACCAG TTGCGTGAGC GAAGATTGAC GCTCCTGTCC TGCTGCAAGG
1141 AAGCTTCAAA ATGGAAAGGA ACCGCCGGGC GG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsumoto, M., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664.

2019.09.10

GNP_filter3_RDBDEP_html_190816.pl