Please review the QC test results indicated by icon below as well as clone information before placing your order.

human TLR3-CFlag expression vector

Expression vector of human TLR3 tagged with FLAG at C-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16911_A9Ajp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(TLR3 5') Sequence file: RDB16911_A9Ajd.seq
>D06031A3_A9Aj_2_EF1a-C_F_A05_02_ABI24.ab1     1 NNNNNNNNNN NNTTTTGAGT TTGGNNCTTG GTTCNTTCTC NAGCCTCAGA CAGTGGTTCA    61 AAGTTTTTTT CTTCCNTTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA   121 GGCCGCCACC ATGAGACAGA CTTTGCCTTG TATCTACTTT TGGGGGGGCC TTTTGCCCTT   181 TGGGATGCTG TGTGCATCCT CCACCACCAA GTGCACTGTT AGCCATGAAG TTGCTGACTG   241 CAGCCACCTG AAGTTGACTC AGGTACCCGA TGATCTACCC ACAAACATAA CAGTGTTGAA   301 CCTTACCCAT AATCAACTCA GAAGATTACC AGCCGCCAAC TTCACAAGGT ATAGCCAGCT   361 AACTAGCTTG GATGTAGGAT TTAACACCAT CTCAAAACTG GAGCCAGAAT TGTGCCAGAA   421 ACTTCCCATG TTAAAAGTTT TGAACCTCCA GCACAATGAG CTATCTCAAC TTTCTGATAA   481 AACCTTTGCC TTCTGCACGA ATTTGACTGA ACTCCATCTC ATGTCCAACT CAATCCAGAA   541 AATTAAAAAT AATCCCTTTG TCAAGCAGAA GAATTTAATC ACATTAGATC TGTCTCATAA   601 TGGCTTGTCA TCTACAAAAT TAGGAACTCA GGTTCAGCTG GAAAATCTCC AAGAGCTTCT   661 ATTATCAAAC AATAAAATTC AAGCGCTAAA AAGTGAAGAA CTGGATATCT TTGCCAATTC   721 ATCTTTAAAA AAATTAGAGT TGTCATCGAA TCAAATTAAA GAGTTTTCTC CAGGGTGTTT   781 TCACGCAATT GGAAGANTAT TTGGCCTCTT TCTGAACAAT GTCCAGCTGG GTCCCAGCCT   841 TACAGANAAN CTATGTTTGG AATTAGCAAA CACAAGCATT CGGAATCTGT CTCTGANTAA   901 CAGCCAGCTG TCCNCCACCA GNNATACNAC TTTCTNGGNN NTNAAGTGGN NAAATCTCAC   961 TANGCTCNNT CTTTCCNANN NCNACTTAAA NGNGGNNGNN NNCGANNNCN TNGNNNNNNN 1021 TCCNNNNNNA NNNNNNTTNN NNNCNNNNNN NNNN //
Primer: hGCSF_R (Pr0031) Region: insert 3'(FLAG,TLR3 3') Sequence file: RDB16911_A9Aje.seq
>D06031A3_A9Aj_2_hGCSF_R_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAN GGTCNNNGGT GGGGNNCCTC ACTCTNNGAG TCGCGGCCGC    61 TTACTTGTCA TCGTCGTCCT TGTAGTCATG TACAGAGTTT TTGGATCCAA GTGCTACTTG   121 CAATTTATGG CGAAAGGCAC CTATCCGTTC TTTCTGAACT GGCCAGTTCA AGATGCAGTG   181 AGATTTAAAC ATTCCTCTTC GCAAACAGAG TGCATGGTTC AGTTTATAAT CTGGAATCTC   241 CTCAAGGAAA ACCAATATAA TGGAATCCAG ATTTTGTTCA ATAGCTTGTT GAACTGCATG   301 ATGTACCTTG AATCTTTTGC ATAATGGGTC TTTTAATAGA TGGTGTGTTA TAACAAAAAT   361 AATTTTTCTG CTTCTTTTGA TGCTGTTAAC AATTGCTTCT AGTTCAAAAA CACCCGCCTC   421 AAAGTCCCTT TCTTCCAGAC AAAATTTGAG AGATTGGTCT TCCTTTTCCA TTGAAGAGAA   481 ATGTTCCCAG ACCCAATCCT TATCTTTATA GGCATGAATT ATATATGCTG CATATTCAAA   541 CTGTTCTGTC TGTCTGTCTA TTTCTTTGAA ACCAAGAACT CGATGTACTG AAACATTCCA   601 ATAAAAAGAT ATCCTCCAGC CCTCAAAGTG GATGAGAAGT ACAATAAAGA TAAAAATCAA   661 CAGGATACTG GTATTGATCA TGAAAAAGAG TTCAAAGGGG GCACTGTCTT TGCAAGATGA   721 TGTATCAAAA AGTCTCACTG GGAACCCATG ATAGTGAGGT GGAGTGTTGC AAANNNAGTG   781 GCTTGACAGC TCAGGGATGT TGGTATGGGT CTCGTTAATC NNATTAACAA ACCAGGCAAT   841 ACTTTCACAC GTGCAATCAA AGGGANTAAA GCGCATATCT NACTCNGTCA GGNTNCTGAA   901 AGCTGGNCCG AAAANNTTCT NCTCNNCNNA TGTTATGANA NNCTTCTGAA NNNTNANTGN   961 NTTTNNAGAN NNCTGANTAT TAAAGANAGA TGCTGGNANN GTGTTTNAAN TNATTNNNTC 1021 CNAAATNGAN GATNNTTANN NNNNNNANNN CNNNNNNNNN N //
Primer: reverse (Pr0099) Region: SV40 pro_ori,EF-1 alpha pro Sequence file: RDB16911_A9Ajf.seq
>D06031A3_A9Aj_2_reverse_C05_08_ABI24.ab1     1 NNNNNNNNNN NNNNNANGCA GAAGTANGCN AAGCATGCAT CTCNATTAGT CAGCAACCAT    61 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC   121 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA   181 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTTG   241 CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA GCTAATGGAC CTTCTAGGTC   301 TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA   361 GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC   421 GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA   481 GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC   541 AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC   601 CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT   661 CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT   721 GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT   781 CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCNTTT AAAATTTTTG ATGACCTGCT   841 GCGACGCTTT TTTTCTGGCN AGATAGTCTT GTAAATGCGG GCCNAGATCT GNACACTGGT   901 ATTTCGGNTT TTGGGGNCGC GGGCGGCGAN NGGGCCNNNN CGNNCCNNCG CNCATGTTCN   961 GNGANNNGGG GNCNGCNANC NNGGNNNNNN ANNNNNNNNN GGGGNNANNN NNNNNCNNGN 1021 NNNGNNNNGN NNNNGNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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