RDB16909 - human TLR3 expression vector

Resource data sheet
Prev.
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

human TLR3 expression vector

Expression vector of human TLR3.

Catalog number	RDB16909
Resource name	human TLR3 expression vector
Clone info.	Expression vector of human Toll-like receptor 3 (TLR3).
Comment	Complementary DNAs for human TLR3 was cloned by RT-PCR from the mRNA of monocyte-derived immature DCs. Expression was confirmed by the depositor with western blotting with anti-TLR3 Ab, flowcytometry with anti-TLR3 mAb (clone TLR3.7).
Vector backbone	pEFBOS (plasmid)
Selectable markers	Amp^r
Gene/insert name	human TLR3 cDNA
Depositor\|Developer	Matsumoto, Misako \|

Other clones in our bank

human TLR3

External Database
human TLR3

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)

Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Order form [Credit Card Payment] [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16909	human TLR3 expression vector	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Matsumoto, M., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16909_A9Ajp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Region: EF1alpha pro,insert5' Sequence file: RDB16909_A9Aja.seq
>D06031A1_A9Aj_1_EF1a-C_F_A04_01_ABI24.ab1 1 TATGGGATGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 121 GGCCGCCACC ATGAGACAGA CTTTGCCTTG TATCTACTTT TGGGGGGGCC TTTTGCCCTT 181 TGGGATGCTG TGTGCATCCT CCACCACCAA GTGCACTGTT AGCCATGAAG TTGCTGACTG 241 CAGCCACCTG AAGTTGACTC AGGTACCCGA TGATCTACCC ACAAACATAA CAGTGTTGAA 301 CCTTACCCAT AATCAACTCA GAAGATTACC AGCCGCCAAC TTCACAAGGT ATAGCCAGCT 361 AACTAGCTTG GATGTAGGAT TTAACACCAT CTCAAAACTG GAGCCAGAAT TGTGCCAGAA 421 ACTTCCCATG TTAAAAGTTT TGAACCTCCA GCACAATGAG CTATCTCAAC TTTCTGATAA 481 AACCTTTGCC TTCTGCACGA ATTTGACTGA ACTCCATCTC ATGTCCAACT CAATCCAGAA 541 AATTAAAAAT AATCCCTTTG TCAAGCAGAA GAATTTAATC ACATTAGATC TGTCTCATAA 601 TGGCTTGTCA TCTACAAAAT TAGGAACTCA GGTTCAGCTG GAAAATCTCC AAGAGCTTCT 661 ATTATCAAAC AATAAAATTC AAGCGCTAAA AAGTGAAGAA CTGGATATCT TTGCCAATTC 721 ATCTTTAAAA AAATTAGAGT TGTCATCGAA TCAAATTAAA GAGTTTTCTC CAGGGTGTTT 781 TCACGCAATT GGAAGATTAT TTGGCCTCTT TCTGAACAAT GTCCAGCTGG GTCCCAGCCT 841 TACAGAGAAG CTATGTTTGG AATTAGCAAA CACAAGCATT CGGAATCTGT CTCTGAGTAA 901 CAGCCAGCTG TCCACCACCA GCAATACAAC TTTCTTGGGA CTAAAGTGGA CAAATCTCAC 961 TATGCTCGAT CTTTCCTACA ACAACTTAAA TGTGGTTGGT AACGATTCCT TTGCTTGGCT 1021 TCCACAACTA GAATATTTCT TTCCTAGAGT ATAATAATAT ACAGCATTTG TTTTTCTCAC 1081 TTCTTTGCAC GGACTTTTTC AATGTGAGTA CCTGATTTGG AACGATCTTT TACCTAAACC 1141 AAAGGTATTT CCCTTGACCT CAACTCCCCC CAAAGAAATG GA //
Primer: hGCSF_R Region: hGCSF p(A),insert3' Sequence file: RDB16909_A9Ajb.seq
>D06031A1_A9Aj_1_hGCSF_R_B04_04_ABI24.ab1 1 CTGGGAAATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGTC GCGGCCGCGA 61 CCTGCAGGCG CAGAACTGGT AGGTATGGAA GATCCCTCGA GATCCATTGT GCTGGCGGTA 121 GGCGAGCAGC GCCTGCCTGA AGCTGCGGGC ATTCCCGATC AGAAATGAGC GCCAGTCGTC 181 GTCGGCTCTC GGCACCGAAT GCGTATGATT CTCCGCCAGC ATGGCTTCGG CCAGTGCGTC 241 GAGCAGCGCC CGCTTGTTCC TGAAGTGCCA GTAAAGCGCC GGCTGCTGAA CCCCCAACCG 301 TTCCGCCAGT TTGCGTGTCG TCAGACCGTC TACGCCGACC TCGTTCAACA GGTCCAGGGC 361 GGCACGGATC ACTGTATTCG GCTGCAACTT TGTCATGCTT GACACTTTAT CACTGATAAA 421 CATAATATGT CCACCAACTT ATCAGTGATA AAGAATCCGC GCCAGCACAA TGGATCTCGA 481 GGAAATTCCG GAATTCGGCT TGTCAAAAAT AGGCCTGAAA TAAGGAGACA CATTTTCCTA 541 GAAGAGATGT AATTGTGATA GAATATAATC ATTTACAAAT ATGAATAAAC AAATTATAAC 601 ACCTTTATGG AAAACTTAAA TTTGCCATAG AACTTTTTAA ATTGAGAAAG TTTCTCCTTT 661 GCTAATTGAA TATTTAAATA AATTTAATGT ACAGAGTTTT TGGATCCAAG TGCTACTTGC 721 AATTTATGGC GAAAGGCACC TATCCGTTCT TTCTGAACTG GCCAGTTCAA GATGCAGTGA 781 GATTTAAACA TTCCTCTTCG CAAACAGAGT GCATGGTTCA GTTTATAATC TGGAATCTCC 841 TCAAGGAAAA CCAATATAAT GGAATCCAGA TTTTGTTCAA TAGCTTGTTG AACTGCATGA 901 TGTACCTTGA ATCTTTTGCA TAATGGGTCT TTTAATAGAT GGTGTGTTAT AACAAAAATA 961 ATTTTTCTGC TTCTTTTGAT GCTGTTAACA ATTGCTTTCT AGTTCAAAAA CACCCGCCTC 1021 AAAGTCCCTT TCTTCCAGAC AAAATTTGAG AGATGGTTCT TCCTTTTCCA TTTGAAGAGG 1081 AAATGTTCCC AGACCCAAAT CCTTATTCTT TATAGCCATG ATTATATATG CCTGCCATAT 1141 CAAACTGTTC TGGTCCTGGT TCTGGTCTTA TTTC //
Primer: reverse Region: SV40pro_ori,EF1alpha pro Sequence file: RDB16909_A9Ajc.seq
>D06031A1_A9Aj_1_reverse_C04_07_ABI24.ab1 1 CATAGTCCTC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT 61 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC 121 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA 181 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTTG 241 CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA GCTAATGGAC CTTCTAGGTC 301 TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA 361 GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC 421 GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA 481 GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC 541 AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC 601 CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT 661 CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT 721 GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT 781 CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT AAAATTTTTG ATGACCTGCT 841 GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG GCCAAGATCT GCACACTGGT 901 ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG CACATGTTCG 961 GCGAGGCGGG GCCTGCGAGC GCGGGCCACC GAGAATCGGG ACGGGGGGTA GTCTCAAGCT 1021 GGGCCGGGCC TGCTCTGGGT GCCTGACCTC GCGCCGTCGT GTATCGCCCC GCCTGGCGGC 1081 CATGCTGGTC TGTCGGCACA GTTGCGTGAA CGGAAGATGG ACGCGTCCTG ATCCTGCCTG 1141 CCAGGTAGCT TCAAAATGTG TAAGGAACGC T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsumoto, M., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl