Please review the QC test results indicated by icon below as well as clone information before placing your order.

pT2b_mT_gvpC20-T2A-mKO2_pP_rm (#RDB16905)

Tol2 transposon expression vector of Planktothrix rubescens gvpC20 and mKO2 marker (C).

Clone info.	Tol2 transposon expression vector of Planktothrix rubescens gvpC20 and mKO2 marker (C).
Comment	Reference requence, AJ132361.1. Expresion was confirmed by measuring fluorescence level of mKO2 using FACS or confocal microscopy.
Vector backbone	pT2/Onc2 (plasmid)
Selectable markers	Amp^r
Gene/insert name	Planktothrix rubescens gvpC20 cDNA
Depositor|Developer	Watanabe, Tomonobu | Mizushima, Ryota |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Mizushima, R. et al., Contrast Media Mol. Imaging 2020: 5425934 (2020)) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the Medical & Biological Laboratories Co., Ltd prior to MTA.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16905	pT2b_mT_gvpC20-T2A-mKO2_pP_rm	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pT2b_mT_gvpC20-T2A-mKO2_pP_rm was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16905).

Reference section:

Mizushima, R., Inoue, K., Fujiwara, H., Iwane, A.H., Watanabe, T.M., Kimura, A., Multiplexed 129 Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells. Contrast Media Mol. Imaging 2020: 5425934 (2020). PMID 32256252. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16905_A9Afp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: beta-gi_F (Pr0497) Region: gvpC20, T2A, mKO2 5' Sequence file: RDB16905_A9Afa.seq
>D05911A3_A9Af_6_beta-gi_F_A12_03_ABI24.ab1     1 NNNNNNNNNN NNCNNNNTNN NCTTTCNNAN NGCTCCTGGG CAACGTGCTG GTTGTTGTGC    61 TGTCTCATCA TTTTGGCAAA GCTAGCATGG CTCTGAAGGA CAAATGGCAG CAGGATAGGA   121 TCGGCAGACA GCAGGGAGTG CAGGAACGGC AGCAGCAGGT CCAGACCACA CTGTCTCTCT   181 GGCAGCAGGA GAGGCAGAAC CAAGCAAGTG AGTTCCGCGA AGACCTGGAG TACCGAGTGA   241 CCGATCTGCT CGCAAATTAT CAGAAGCAGC GACTGGAAGC CAGGGAGACA CTGCTCGAAG   301 ACCTCGCTAT CTTTAGACAG ACTCTGTACC GGGAAGTCGA GGAATATCTG GGCGAGCTCG   361 ATATTCTGCA CCAGCAGATG GCCGCTCAGC TCCAGCAGCA GCTGCAGCAG AGCCGGACCG   421 AGCGAAAGGA CGCAGTGCAG AAACTCTTCG AAGATCTGGG CGTCTTTAGG GCCGAGCTGC   481 AGGACTACCA TCTGAAGCTC CAGCAGACAG TGTGGGGAAG CTCCCACAGA CATAGCATCA   541 AGCCCCAGCC TACTGTGAAC CCTGGAGTCC CACAGCCAGC TACCCTGGAT CAGCCACAGG   601 GTGAATTCGA GGGCAGAGGA AGTCTTCTAA CATGCGGTGA CGTGGAGGAG AATCCCGGCC   661 CTATGGTGAG CGTGATCAAG CCCGAGATGA AGATGAGGTA CTACATGGAC GGCTCCGTCA   721 ATGGGCATGA GTTCACAATC GAGGGTGANG GCACAGGCAG ACCTTACGAG GGACATCAGG   781 AGATGACACT GCGCGTCACA ATGGCCGANG GCGGGCCAAT GCCTTTCGCC TTCNACCTGG   841 TGTCCCACGN NTTCTGTTAC NGNCACNGAG TTTTTACTAA ATATCCNGAA NANATNCCAG   901 ANNATTTCNN GCNNGNCNTT CCTGAGGGNC NGNCCTGGGA NANGNCCCNG GNNTTNNANG   961 NNNNNGCTNN NNNNCCNNNN NCNNNNNNNT CNNCNNGNNG GNNNNCNNCN TNNNNNNNNN 1021 N //
Primer: PGK_N_rev (Pr0243) Region: BGH pA_1, mKO2 3' Sequence file: RDB16905_A9Afb.seq
>D05911A3_A9Af_6_PGK_N_rev_A11_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCNCNNAAG CCNTAGAGCC CACCGCATCC CCAGCATGCC    61 TGCTATTGTC TTCCCNATCC TCCCCCTTGC TGTCCTGCCC CACCCCACCC CCCAGAATAG   121 AATGACACCT ACTCAGACAA TGCGATGCAA TTTCCTCATT TTATTAGGAA AGGACAGTGG   181 GAGTGGCACC TTCCAGGGTC AAGGAAGGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC   241 AACTAGAAGG CACAGTCGAG GCTGATCAGC GAGCTCTAGA TCATCGATCA AGCTTATCGA   301 GCGGCCGCTT AGGAGTGGGC CACGGCGTCC TCCACCTGCT CGGTGATGTT GCCCTCGGTC   361 TTCCTCACCA GCCTGTGGCC GATGTAGTGG TCGCCGGGCA TCTCCAGGAT CTCCTTGGCG   421 GCCTTGTAGG TGGTCTTCAT CTGGCACTTG TGGTTGCCGC CGCCCTCCAG CTTCAGGTAC   481 ATGGTCACGT CGCCCTTCAG CACGCCGTCG CTGGCGGTGA TCTTCTCGGT GGAGGGCTCC   541 CAGTCCACGC TCTGGTTCTG CATGATGGGG CCGTCGGCGG GGAAGTTCAC GCCGGTGAAC   601 TTGGACTTGT GGTAGAAGGT GTTGCCCCTC AGGCTGATGT GGGCGCTCAC GGAGGCGGAG   661 CCGCCGTCCT CGAACTCCAG GGACCTCTCC CAGGACAGGC CCTCAGGAAA GGNCTGCTTG   721 AAATAGTCTG GGATCTCTTC TGGATATTTA GTAAAAACTC TGTGGCCGTA ACAGAACACG   781 TGGGANACCA GGTCGAANGN GAAAGGNATT GGCCCGCCCT CGGCCATTGT GACGCGCANT   841 GTCNTCTCCT GATGTCCCTC GTAANGNCTG CCNGNGCCCT CACCCTCNAN TGNGAACTCA   901 TGNCCATTGA CNGANCCNTC CNNGTANTAN CNCATCTTCA TCTCGGGCTN GATCACNCTC 961 ANCNTNNNNC NNGNNNTNNN NNNNNCNNCN NNNNNNNNN //
Primer: PGKpro_F (Pr0249) Region: PuroR, BGH pA_2 Sequence file: RDB16905_A9Afc.seq
>D05911A3_A9Af_6_PGKpro_F_B11_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNN NNNTCTCCGG GCCTTTCGAC CTGTGGCCNC AACCNTGACC    61 GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC   121 CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC   181 ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC   241 AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC   301 GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG   361 CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG   421 TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC   481 GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC   541 TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC   601 GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG   661 CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC GATGATCTAG   721 AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC   781 CCCCGTGCCT TCCTTGACCC TGGAANGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA   841 GGAAATTGCA TCGCATTGTC TGANTANGTG TCATTCTATT CTGGGGGGTG GGGTNNNNAN   901 GNNAGCAAGG GGGANGANTG GGAAGANAAT ANCNNNNTGC TGGGGNNGNN GNGGGCNCNA 961 TGGCNGCNNC CNNNNCTTNN NNNNNNCNTN GNNCATANCT GTTTNCNNGN NNNNNNNNN //
Primer: f1ori_R3 (Pr0142) Region: Left Tol2, I-SceI, cHS4 ins_1 Sequence file: RDB16905_A9Afd.seq
>D05911A3_A9Af_6_f1ori_R3_F12_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCNNTTGG GANGGCGATC GGTGCGGGCC TCTTCGCTAT    61 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT   121 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG   181 GCGAATTGGG TACCAGAGGT GTAAAGTACT TGAGTAATTT TACTTGATTA CTGTACTTAA   241 GTATTATTTT TGGGGATTTT TACTTTACTT GAGTACAATT AAAAATCAAT ACTTTTACTT   301 TTACTTAATT ACATTTTTTT AGAAAAAAAA GTACTTTTTA CTCCTTACAA TTTTATTTAC   361 AGTCAAAAAG TACTTATTTT TTGGAGATCA CTTGTCGAGT AGGGATAACA GGGTAATGAT   421 CCCCCGGGCT GCAGTCGCGA CGCGTCCTAG AGGGACAGCC CCCCCCCAAA GCCCCCAGGG   481 ATGTAATTAC GTCCCTCCCC CGCTAGGGGG CAGCAGCGAG CCGCCCGGGG CTCCGCTCCG   541 GTCCGGCGCT CCCCCCGCAT CCCCGAGCCG GCAGCGTGCG GGGACAGCCC GGGCACGGGG   601 AAGGTGGCAC GGGATCGCTT TCCTCTGAAC GCTTCTCGCT GCTCTTTGAG CCTGCAGACA   661 CCTGGGGGGA TACGGGGAAA AAGCTTTAGG CTGAAAGAGA GATTTAGAAT GACAGAATCA   721 TAGAACGGCC TGGGTTGCAA AGGAGCACAG TGCTCATCCA GATCCAACCC CCTGCTATGT   781 GCANGGTCAT CAACCAGCAG CCCAGGCTGC CNNNNNCCAC ATCCNNNCTG NCCTTGAATG   841 CCTGCAGGGA NGGGGCATCC NCAGCCTCCT NNGNCAACCT GNNCAGTGNG TCANCACCCT   901 CTGGGGGAAA AACTGCCNNC NCNTATCNNA NCCAANNNTC CCCTGTCTCN NNGNAAAGNC   961 ATTCCCCCTT GNNCTATNNN GGGGGANTTT GNTGTGNNNT TNNNGGNCTG GGGNNNNNCA 1021 TNTTTNNCNA NNNNNNNNNA TNNNNNNNNN N //
Primer: beta-gro_rev (Pr0512) Region: mini CMV pro, tet RE, pA, cHS4 ins_1 Sequence file: RDB16905_A9Afe.seq
>D05911A3_A9Af_6_beta-gro_rev_C12_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNANNNNT TNNNNNNNNN NATAANNTGA ATTTTACAAT    61 AGCGAAAAAG AAAGAACAAT CAAGGGTCCC CAAANTCNNN CTGAAGTTCT CAAGGCTGGA   121 TCGGTCCCGG TGTCTTCTAT GGAGGTCAAA ACAGCGTGGA TGGCGTCTCC AGGCGATCTG   181 ACGGTTCACT AAACGAGCTC TGCTTATATA GGCCTCCCAC CGTACACGCC TACCTCGACC   241 CGGGTACCGA GCTCGACTTT CACTTTTCTC TATCACTGAT AGGGAGTGGT AAACTCGACT   301 TTCACTTTTC TCTATCACTG ATAGGGAGTG GTAAACTCGA CTTTCACTTT TCTCTATCAC   361 TGATAGGGAG TGGTAAACTC GACTTTCACT TTTCTCTATC ACTGATAGGG AGTGGTAAAC   421 TCGACTTTCA CTTTTCTCTA TCACTGATAG GGAGTGGTAA ACTCGACTTT CACTTTTCTC   481 TATCACTGAT AGGGAGTGGT AAACTCGACT TTCACTTTTC TCTATCACTG ATAGGGAGTG   541 GTAAACTCGA TGTAAGCTTG CCGTTGTCGA CCACACAAAA AACCAACACA CAGATGTAAT   601 GAAAATAAAG ATATTTTATT CCTAGAGCCC CATCCTCACT GACTCCGTCC TGGAGTTGGA   661 TGAGAGATAA TGGCCTTACG TTGTGCCAGG GGAGGGTCGG GCTGGATTTA GCNAAGATTT   721 ACCTTCTCCA AAGAGCGGTG CTGCAGTGGC ACAGCTGCCC ACGGAGGTGG GGGGGTCACC   781 GTCCCTGGAG GTGATGAANA ACTGTGGGGA NGTGGCACTG ANGGACATGG CCAGTGGGCN   841 NNNGNGGGTG GGGTTGGGGN NGGNCTTGGG NANCNNGGAG GGCTTTTNNN NNNTTCATGN   901 ANTTNNNCNA ATNNNATGAA NCNTCTACNT GGGNNNTTNN NCNNCTGNCN NGTCNCAGTC 961 NNACTGNNCN NANCTNNNNN NNNNN //
Primer: tetR-C_F2 (Pr0862) Region: tetR, SV40 NLS, KRAB Sequence file: RDB16905_A9Aff.seq
>D05911A3_A9Af_6_tetR-C_F2_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNTCG GGCTNNNNNC ATTAATATTT    61 GGATTTTCTG CACAGTTNNA TGAAAAAAAG CATACACCCA TTGAGGATGG TAATAAATCG   121 CCAAAAAAGA AGAGAAAGGT CGATGGCGGT GGTGCTTTGT CTCCTCAGCA CTCTGCTGTC   181 ACTCAAGGAA GTATCATCAA GAACAAGGAG GGCATGGATG CTAAGTCACT AACTGCCTGG   241 TCCCGGACAC TGGTGACCTT CAAGGATGTA TTTGTGGACT TCACCAGGGA GGAGTGGAAG   301 CTGCTGGACA CTGCTCAGCA GATCGTGTAC AGAAATGTGA TGCTGGAGAA CTATAAGAAC   361 CTGGTTTCCT TGGGTTATCA GCTTACTAAG CCAGATGTGA TCCTCCGGTT GGAGAAGGGA   421 GAAGAGCCCT GGCTGGTGGA GAGAGAAATT CACCAAGAGA CCCATCCTGA TTCAGAGACT   481 GCATTTGAAA TCAAATCATC AGTTTAAGGA TCCGAGCTCG GTGATCCGCT GGCACGACAG   541 GTTTCCCGAC TGGAAAGCGG GCANNNNNNG CAACGCAATT AATGTGAGTT AGCTCACTCA   601 TTANGCACCC CAGGCNNNNN NNTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG   661 CGGATAACAA TTTCANNNNN NAAACAGCTA TGACCNTGAT TACGCCAAGC TTGGGCTGCA   721 GCCATAGAGC CCACCGCATC CCCAGCATGN CNTGCTATTG TCTTCCCNAA TCCTCCCCCT   781 TGCTGTCCTG CCCCACCCCA NCCCCCANAA NAGAATGACN CCTACTCANA NNATGCNANN   841 NNATTTCCTC ANTTTATNAG GAANNGANAG TGGGNANNGG CNCCNTNNCA GGGTNNNAGG   901 NAGGCNCNGG GGGNNGGNNN AAANNANCAN ANGGCNGGNN AACNNNAANG NNNANTCNNN   961 GGCNNNATCA NNNNGCNNNN NNNATCATCN ANNNNCANNG GGGNNNNNNG NNNNNCCTTN 1021 NNNNNNGGNN NNNNNNGGNN NNNNGGGGGN NGNNNNNNNN GNNNNNNGGN NNNNNNNNN //
Primer: IRES-F (Pr0036) Region: IRES, tetR, SV40 NLS Sequence file: RDB16905_A9Afg.seq
>D05911A3_A9Af_6_IRES-F_H12_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNN GTCGAGGTTN NNNNNNGTCT AGGCCCCCCG AACNACGGGG    61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCTA GATTAGATAA AAGTAAAGTG   121 ATTAACAGCG CATTAGAGCT GCTTAATGAG GTCGGAATCG AAGGTTTAAC AACCCGTAAA   181 CTCGCCCAGA AGCTTGGTGT AGAGCAGCCT ACATTGTATT GGCATGTAAA AAATAAGCGG   241 GCCCTTATGA ACATGCTTTC AGAGGCAATA CTGGCGAAGC ATCACACCCG TTCAGCACCG   301 TTACCGACTG AGAGTTGGCA GCAGTTTCTC CAGGAAAATG CTCTGAGTTT CCGTAAAGCA   361 TTACTGGTCC ATCGTGATGG AGCCCGATTG CATATAGGGA CCTCTCCTAC GCCCCCCCAG   421 TTTGAACAAG CAGAGGCGCA ACTACGCTGT CTATGCGATG CAGGGTTTTC GGTCGAGGAG   481 GCTCTTTTCA TTCTGCAATC TATCAGCCAT TTTACGTTGG GTGCAGTATT AGAGGAGCAA   541 GCAACAAACC AGATAGAAAA TAATCATGTG ATAGACGCTG CACCACCATT ATTACAAGAG   601 GCATTTAATA TTCAGGCGAG AACCTCTGCT GAAATGGCCT TCCATTTCGG GCTGAAATCA   661 TTAATATTTG GATTTTCTGC ACAGTTAGAT GAAAAAAAGC ATACACCCAT TGAGGATGGT   721 AATAAATCGC CAAAAAAGAA GAGAAAGGTC GATGGCGGTG GTGCTTTGTC TCCTCAGCAC   781 TCTGCTGTCA CTCAAGGAAG TATCATCAAG AACAAGGAGG GCATGGNTGC TAAGTCACTA   841 ACTGCCTGGT CCCNGACACT GGTGANCTTC AAGGANGTAT TTGTGGANNT CNNNNNGGAN   901 GANTGGAAGC TGCTGGANNC TGCTCANCAN ATCGNGTACA GAAATGTGAT GCTGGANAAC   961 TNTAANAANN NGGNTTCCNN GGGTNANCNN CNTNNTNANN NNGANGTGAT NNNNNNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: SV40 pro_ori, rtTA-Advenced Sequence file: RDB16905_A9Afh.seq
>D05911A3_A9Af_6_SV40pro_F_V2_D11_11_ABI24.ab1     1 NNNNNNNNNN NNNTNNNNNN NNNNNNNNNC TTTTGCAAAA AGCTCTCTGG CTAACTAGAG    61 AACCCACTGC TTACTGGCTT ATCGAAATTA ATACGACTCA CTATAGGGAG ACCCAAGCTG   121 CAATTCACCA TGAGCAGACT GGACAAGAGC AAAGTCATAA ACGGCGCTCT GGAATTACTC   181 AATGGAGTCG GTATCGAAGG CCTGACGACA AGGAAACTCG CTCAAAAGCT GGGAGTTGAG   241 CAGCCTACCC TGTACTGGCA CGTGAAGAAC AAGCGGGCCC TGCTCGATGC CCTGCCAATC   301 GAGATGCTGG ACAGGCATCA TACCCACTTC TGCCCCCTGG AAGGCGAGTC ATGGCAAGAC   361 TTTCTGCGGA ACAACGCCAA GTCATTCCGC TGTGCTCTCC TCTCACATCG CGACGGGGCT   421 AAAGTGCATC TCGGCACCCG CCCAACAGAG AAACAGTACG AAACCCTGGA AAATCAGCTC   481 GCGTTCCTGT GTCAGCAAGG CTTCTCCCTG GAGAACGCAC TGTACGCTCT GTCCGCCGTG   541 GGCCACTTTA CACTGGGCTG CGTATTGGAG GAACAGGAGC ATCAAGTAGC AAAAGAGGAA   601 AGAGAGACAC CTACCACCGA TTCTATGCCC CCACTTCTGA GACAAGCAAT TGAGCTGTTC   661 GACCGGCAGG GAGCCGAACC TGCCTTCCTT TTCGGCCTGG AACTAATCAT ATGTGGCCTG   721 GAGAAACAGC TAAAGTGCGA AAGCGGCGGG CCGGCCGACG CCCTTGACGA TTTTGACTTA   781 NACATGCTCC CAGCCGATGC CCTTGACGAC TTTGANCTTG ATATGCTGCC TGCTGACGCT   841 CTTGACGANT TTGACCTTGA CATGCTCCCC GGGNAACTAA GTAAGGANCA TCCNCCCCCC   901 CCCCCTAACG TTACTGGNCG AANCCGCTNG NAATNNNGNC GGNGNGNGTT TGNCTATATG   961 TTATTTTNCN NCNTNNTGNC GNCTTTTNGN NNTGNGANGG NCNNGNAANN CNTNNNNNNN 1021 NNN //
Primer: SV40pA3'Element reverse (Pr0108) Region: Right Tol2, I-SceI, cHS4 ins_2 Sequence file: RDB16905_A9Afi.seq
>D05911A3_A9Af_6_SV40pA3'element Reverse_G12_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN NNATGNNNNN NNNCNNGCAA CGCGGCCTTT TTACGGTTCC    61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG   121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC   181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG   241 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA   301 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT   361 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA   421 ACAGCTATGA CCATGATTAC GCCAAGCTCG AAATTAACCC TCACTAAAGG GAACAAAAGC   481 TGGAGCTCCA GAGGTGTAAA AAGTACTCAA AAATTTTACT CAAGTGAAAG TACAAGTACT   541 TAGGGAAAAT TTTACTCAAT TAAAAGTAAA AGTATCTGGC TAGAATCTTA CTTGAGTAAA   601 AGTAAAAAAG TACTCCATTA AAATTGTACT TGAGTATTGG ATCAATTACC CTGTTATCCC   661 TATCGAGACG CGTCGGTCCG GCTAGAGCCC CATCCTCACT GACTCCGTCC TGGAGTTGGA   721 TGAGAGATAA TGGCCTTACG TTGTGCCAGG GGAGGGTCGG GCTGGATTTA GCAAGATTTA   781 CCTTCTCCAA AGAGCGGTGC TGCAGTGGCA CAGCTGCCCA CGGANGTNGG GGGGTCACCG   841 TCCCTGGANG TGATGAANAN NTGTGGGGAN GTGGCACTGA NGGANATGGC CAGTGGGCAC   901 GGNGGGTNGG TTGGGGTNNG NCTTGGGGNN CTTGGANGGC TTTTNCAGCC TTCATGATTT   961 GACNANTGTA TNNNNTCNNC NTGGCANNTN NNCNNCTGNN TGNCCNNNNN CNANTGNNCN 1021 ANCNNNNTCT CTNNNNNNNN NN //
Primer: SV40pro_R (Pr0470) Region: SV40 pro_ori, cHS4 ins_2 Sequence file: RDB16905_A9Afj.seq
>D05911A3_A9Af_6_SV40pro_R_C04_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNNGNNNNN NANNNNGGGC GGANCTGGGC    61 GGANTTAGGG GCGNNNNGNG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA   121 TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT   181 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA   241 CACCCTAACT GACACACATT CCACAGCCGT TAATTAAGAT CCCTAGAGGG ACAGCCCCCC   301 CCCAAAGCCC CCAGGGATGT AATTACGTCC CTCCCCCGCT AGGGGGCAGC AGCGAGCCGC   361 CCGGGGCTCC GCTCCGGTCC GGCGCTCCCC CCGCATCCCC GAGCCGGCAG CGTGCGGGGA   421 CAGCCCGGGC ACGGGGAAGG TGGCACGGGA TCGCTTTCCT CTGAACGCTT CTCGCTGCTC   481 TTTGAGCCTG CAGACACCTG GGGGGATACG GGGAAAAAGC TTTAGGCTGA AAGAGAGATT   541 TAGAATGACA GAATCNNNNN ACGGCCTGGG TTGCAAAGGA NNNNNNTGCT CATCCAGATC   601 CAACCCCCTG CTATGTGCAG GGTCATCAAC CAGCAGCCCA GGCTGCCCAG AGCCNNNTNN   661 NNNCTGGCCT TGAATGCCTG NNNNGATGGG GCATCCACNN NCTCCTTGGG CAACCTGTTC   721 NGTGCGTCAC CACCCTCTGG GGGAAAAAAN CTGCCTCCTC ATATCCAACC CNNAAACCTC   781 CCCTGNCTCA GTGNNAAAGC CATTCCCCCC NTTGTCCTAT CAAGGGGGGG AGTTTGCTGN   841 NNCATNGNTT GGTCTGGGNG GTGANNNCAT GTTNGNCNNA NTTCAGTNGC ATCNNNNNNN   901 NGNNNNATCT TTNNGGNATN AANGNNAGTN NCANGNNNNN CATGGNNNNN NGGNNNNNNG 961 NCNNNNNNNT TNNAANGGNC NNNNNGNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Mizushima, R., Multiplexed 129 Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells. Contrast Media Mol. Imaging 2020: 5425934 (2020). PMID 32256252. [link to RRC of NBRP]

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External Database
Planktothrix rubescens gvpC20

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Reference sequence
  

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