Resource data sheet
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pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

A donor vector for introducing Tet-OsTIR1 at the human AAVS1 locus.

Catalog number RDB16812
Resource name pMK365 (Tet-OsTIR1-loxP-PURO-loxP)
Clone info. A donor vector for introducing Tet-OsTIR1 at the human AAVS1 locus. Oryza sativa OsTIR1 (NP_001052659.1), codon optimized cDNA for the expression in human cells.
Vector backbone plasmid
Selectable markers Am^r (E. coli), Puro^r (mammalian cells)
Gene/insert name human AAVS1 genomic Oryza sativa OsTIR1 cDNA
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB16812 pMK365 (Tet-OsTIR1-loxP-PURO-loxP) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16812_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Pause site_R RDB16812_A8Lpa.seq
>D06157A8_A8LP_2_Pause site_R_C02_08_ABI24.ab1
    1 CAGGACCAAT TTGCTAGTTG TTTTGTTTCG TTTTGTTTTG ATGGAGAGCG TATGTTAGTA
   61 CTATCGATTC ACACAAAAAA CCAACACACA GATGTAATGA AAATAAAGAT ATTTTATTCT
  121 CGAGGTGATA ATTCCACGGG GTTGGGGTTG CGCCTTTTCC AAGGCAGCCC TGGGTTTGCG
  181 CAGGGACGCG GCTGCTCTGG GCGTGGTTCC GGGAAACGCA GCGGCGCCGA CCCTGGGTCT
  241 CGCACATTCT TCACGTCCGT TCGCAGCGTC ACCCGGATCT TCGCCGCTAC CCTTGTGGGC
  301 CCCCCGGCGA CGCTTCCTGC TCCGCCCCTA AGTCGGGAAG GTTCCTTGCG GTTCGCGGCG
  361 TGCCGGACGT GACAAACGGA AGCCGCACGT CTCACTAGTA CCCTCGCAGA CGGACAGCGC
  421 CAGGGAGCAA TGGCAGCGCG CCGACCGCGA TGGGCTGTGG CCAATAGCGG CTGCTCAGCA
  481 GGGCGCGCCG AGAGCAGCGG CCGGGAAGGG GCGGTGCGGG AGGCGGGGTG TGGGGCGGTA
  541 GTGTGGGCCC TGTTCCTGCC CGCGCGGTGT TCCGCATTCT GCAAGCCTCC GGAGCGCACG
  601 TCGGCAGTCG GCTCCCTCGT TGACCGAATC ACCGACCTCT CTCCCCAGGG GGATCATCGA
  661 ATTACCATGT CTAGACTGGA CAAGAGCAAA GTCATAAACT CTGCTCTGGA ATTACTCAAT
  721 GGAGTCGGTA TCGAAGGCCT GACGACAAGG AAACTCGCTC AAAAGCTGGG AGTTGAGCAG
  781 CCTACCCTGT ACTGGCACGT GAAGAACAAG CGGGCCCTGC TCGATGCCCT GCCAATCGAG
  841 ATGCTGGACA GGCATCATAC CCACTCCTGC CCCCCTGGAA AGGCGAGTCA TGGCAAGACT
  901 TTCTGCGGAA CAACGCCCAA GTCATACCGC TGTGCTCTCC TCTCACATCG CGACGGGGCT
  961 AAGTGCATCT CGGCACCCGC CCAACAGAGA AACAGTACGA ACCCTGGTAA AATCAGCTCG
 1021 CGTTCCTGTG TCAGCAACGC CTCTCCCTGC AGAACGCACT GTACGCTCTG GTCCCGTTCG
1081 TGGGACCACT TTAACCACTT
//
Primer: PGK_N_rev RDB16812_A8Lpb.seq
>D06157A8_A8LP_2_PGK_N_rev_D02_11_ABI24.ab1
    1 CCAAGTACGC TCCCTACCCG GTAGATTTCG AGGTCGAGAT CCTAAGCTTG GCTGGACGTA
   61 AACTCCTCTT CAGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG
  121 TTATTGAATA TGATCGCCCC GAAAAGTGCC ACCTGACGTC GGCAGTGAAA AAAATGCTTT
  181 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA
  241 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT
  301 TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCC TCTAGACATA
  361 TGCTGCAGAG ATCTGGATCC ACCGGTATCG ATACGCGTGT CGACGGGCCC GGGATCCGAT
  421 GGATCCTCAC AGAATCTTCA CAAAGTTGGG AGCATCATCT CTTGCCCCTG CGGTGGTCCG
  481 ATACACATAC AGTTTCTCCA CCTTTGGCAG GTCGCCATGA TTTTCCTCCA TTTCGTTAGA
  541 CCCGTCCCGC TCATTAATGA CTTCCACGTT CAGCATAGGC ATTTTACTAG CCAGGACCTG
  601 ACAGCCCTTC AGAGTCACAT TGCAACTAGA CATCCACAGG CTCCTCATTG TCTCGTATCT
  661 GGCAAAGTTT CCCAGCAGAG CGGCATCCCC GAAAGGGCTG TCCCGGATTT CCAGTTTTCG
  721 CAGATTCTTA CACCCGTTCA TCACGTGCAT CATGCCCTTA TCGGAGTCTC CGGCAAATGC
  781 AATGCTCAGC ATCTCCAGCT GCTTAGCATA CTTGCCGATG TACATGAACA CTTTGTCTGT
  841 CAGCAGGCCG GAAATGCTCA GCCTCTGGAG TCCCTTGCAC TCTCTCACGA TAGCTCCAAA
  901 CCCTTTCATC CAGTGGCTGG GATGTGACCA CGTCGGGTTT GCCTGGGTTC CAGAATACAC
  961 AGCCCGAAAT CGGGTGAAAA TTAGGGCAGT TTCTTTGACG ACGGTCACCA AGTGCAGCAT
 1021 TTAGTCATCT GATGACAGAA ATACAGGCAG GCTGGATTCA GCTTGGCCAT TCCTAGTGGA
1081 GACTGACCCA CCCAAGGACG ATC
//
Primer: synthPA_F RDB16812_A8Lpc.seq
>D06157A8_A8Lp_2_synthPA_F_E07_13_ABI24.ab1
    1 TGTCAGGATT AGTAACTAAC ATACGCTCTC CATCAAAACA AAAACGAAAA CAAAACAAAC
   61 TAGCAAAATA GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG AACATTTCTC TCTCGAGTTT
  121 ACTCCCTATC AGTGATAGAG AACGTATGAA GAGTTTACTC CCTATCAGTG ATAGAGAACG
  181 TATGCAGACT TTACTCCCTA TCAGTGATAG AGAACGTATA AGGAGTTTAC TCCCTATCAG
  241 TGATAGAGAA CGTATGACCA GTTTACTCCC TATCAGTGAT AGAGAACGTA TCTACAGTTT
  301 ACTCCCTATC AGTGATAGAG AACGTATATC CAGTTTACTC CCTATCAGTG ATAGAGAACG
  361 TATAAGCTTT GCTTATGTAA ACCAGGGCGC CTATAAAAGA GTGCTGATTT TTTGAGTAAA
  421 CTTCAATTCC ACAACACTTT TGTCTTATAC CAACTTTCCG TACCACTTCC TACCCTCGTA
  481 AAGAATTCGC GGCCGCACGC GAAGCGCTGC CACCATGACA TACTTTCCTG AAGAGGTCGT
  541 CGAACACATT TTTAGCTTCC TGCCTGCACA GAGAGATAGA AACACAGTGA GCCTGGTCTG
  601 CAAAGTGTGG TACGAGATCG AACGCCTGAG CCGGAGAGGA GTGTTCGTCG GCAACTGCTA
  661 TGCTGTGAGA GCAGGCAGGG TCGCCGCTAG GTTTCCAAAT GTGCGCGCAC TGACCGTCAA
  721 GGGGAAACCC CACTTCGCCG ACTTTAACCT GGTGCCCCCT GATTGGGGAG GATACGCCGG
  781 CCCTTGGATC GAGGCAGCCG CTCGCGGCTG TCATGGACTG GAGGAACTGC GCATGAAGCG
  841 AATGGTGGTC TCTGACGAAA GTCTGGAGCT GCTGGCTCGG GAGCTTCCCT AGGTTTCGCG
  901 CACTGGTGCT GATTTCCTTG CGAAAGGCTT CAGCACCGAT GGACTGGCAG CCCGTGGCCT
  961 CCCAACTGTA AGCTGCTGCG GGAGCTGGAA CCTTCCAGGA AGAATGAAAG TGGAAGGAAT
 1021 AGAGGGCCCC AGATTGGCTG TCTTGCCTTT CCCTAGACTC AATGTAACCA GTCCTGGATG
1081 TCCCTTGGAA CCTTTGGCTG GCAATCCAAA GGCCTAAGTT GAATGCCCTG GGGATTC
//
Primer: PGKpro_F RDB16812_A8Lpd.seq
>D06157A8_A8LP_2_PGKpro_F_F02_17_ABI24.ab1
    1 ACTTCGGGCC GATCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCATCCA TCTAGATCTC
   61 GAGCAGCTGA AGCTTACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC
  121 GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC
  181 CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC
  241 ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG
  301 GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC
  361 ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG
  421 CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC
  481 CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC
  541 GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCACAACC TCCCCTTCTA CGAGCGGCTC
  601 GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC
  661 CGCAAGCCCG GTGCCTGACG CCCGCCCCAC GACCCGCAGC GCCCGACCGA AAGGAGCGCA
  721 CGACCCCATG CATCGATGAT ATCAGATCCC CGGGATGCAG AAATTGATGA TCTATTAAAC
  781 AATAAAGATG TCCACTAAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA GAAGGGTGAG
  841 AACAGAGTAC CTACATTTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG GGGTGGGGGT
901 GGGA
//
Primer: Tet-On 3G C_F RDB16812_A8Lpe.seq
>D06157A8_A8LP_2_Tet-On 3G C_F_G02_20_ABI24.ab1
    1 TGGCGGCTAG GTGCGAAGCG GCGGGCCGAC CGACGCCCTT GACGATTTTG ACTTAGACAT
   61 GCTCCCAGCC GATGCCCTTG ACGACTTTGA CCTTGATATG CTGCCTGCTG ACGCTCTTGA
  121 CGATTTTGAC CTTGACATGC TCCCCGGGTA AATCTCCAGA GGATCATAAT CAGCCATACC
  181 ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA
  241 CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA
  301 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCC CCGAGCTTCC
  361 TCGCTCACTG ACTCAGCTTG CCCCACTGTG GGGTGGAGGG GACAGATAAA AGTACCCAGA
  421 ACCAGAGCCA CATTAACCGG CCCTGGGAAT ATAAGGTGGT CCCAGCTCGG GGACACAGGA
  481 TCCCTGGAGG CAGCAAACAT GCTGTCCTGA AGTGGACATA GGGGCCCGGG TTGGAGGAAG
  541 AAGACTAGCT GAGCTCTCGG ACCCCTGGAA GATGCCATGA CAGGGGGCTG GAAGAGCTAG
  601 CACAGACTAG AGAGGTAAGG GGGGTAGGGG AGCTGCCCAA ATGAAAGGAG TGAGAGGTGA
  661 CCCGAATCCA CAGGAGAACG GGGTGTCCAG GCAAAGAAAG CAAGAGGATG GAGAGGTGGC
  721 TAAAGCCAGG GAGACGGGGT ACTTTGGGGT TGTCCAGAAA AACGGTGATG ATGCAGGCCT
  781 ACAAGAAGGG GAGGCGGGAC GCAAGGGAGA CATCCGTCGG AGAAGGCCAT CCTAAGAAAC
  841 GAGAGATGGC ACAGGCCCCA GAAGGAGAAG GAAAAGGGAA CCCAGCGAGT GAAGACGGCA
  901 TGGGGTTGGG TGAGGGAGGA GAGATGCCCG GAGAGGACCC AGACACGGGG AGGATCCGCT
  961 CAGAGGGACA TCACGTGGTG CAGCGCCCGA GAAGAAAGTG CTCCGGGAAA GAGCATCCCT
 1021 TGGGCAGCAC ACAGCAGAGA GCAAGGGGAG AGGAGTGTAG GAGACGGATC TGGACGAAGC
1081 GGCAGGGAAG GAATCCTG
//
Primer: PuroR_F RDB16812_A8Lpf.seq
>D06157A8_A8Lp_2_PuroR_F_E01_05_ABI08.ab1
    1 GGCCTTTTTC CGGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC
   61 AAGCCCGGTG CCTGCACTGC CCGCCCCACG ACCCGCAGCG CCCGACCGAA AGGAGCGCAC
  121 GACCCCATGC ATCGATGATA TCAGATCCCC GGGATGCAGA AATTGATGAT CTATTAAACA
  181 ATAAAGATGT CCACTAAAAT GGAAGTTTTT CCTGTCATAC TTTGTTAAGA AGGGTGAGAA
  241 CAGAGTACCT ACATTTTGAA TGGAAGGATT GGAGCTACGG GGGTGGGGGT GGGGTGGGAT
  301 TAGATAAATG CCTGCTCTTT ACTGAAGGCT CTTTACTATT GCTTTATGAT AATGTTTCAT
  361 AGTTGGATAT CATAATTTAA ACAAGCAAAA CCAAATTAAG GGCCAGCTCA TTCCTCCCAC
  421 TCATGATCTA TAGATCTATA GATCTCTCGT GGGATCATTG TTTTTCTCTT GATTCCCACT
  481 TTGTGGTTCT AAGTACTGTG GTTTCCAAAT GTGTCAGTTT CATAGCCTGA AGAACGAGAT
  541 CAGCAGCCTC TGTTCCACAT ACACTTCATT CTCAGTATTG TTTTGCCAAG TTCTAATTCC
  601 ATCAGAAGCT GGTCGAGATC CGGAACCCTT AATATAACTT CGTATAATGT ATGCTATACG
  661 AAGTTATTAG GTCCCTCGAA GAGGTTCACT AAGCTTTACT AGGGACAGGA TTGGTGACAG
  721 AAAAGCCCCA TCCTTAGGCC TCCTCCTTCC TAGTCTCCTG ATATTGGGTC TAACCCCCAC
  781 CTCCTGTTAG GCAGATTCCT TATCTGGTGA CACACCCCCA TTTCCTGGAG CCATCTCTCT
  841 CCTTGCCAGA ACCTCTAAGG TTTGCTTACG ATGGAGCCAG AGAGGATCCT GGGAGGGAGA
  901 GCTTGGCAGG GGGGTGGGAG GGAAGGGGGG GGATGCGTGA CCTGCCCGGG TTCTCAGTGG
  961 CCACCCTGCG CTACCCTCTC CCAGAACCCT GAGCTGCTCT GACGCGGCTG TCTGGGTGCG
 1021 TTTCACTGAT CCCTGGGTGC TGCAGCTTCC CTTACACTCC CAGAGGAGAA GCAGTTTGGA
 1081 AAACAAAATC AGGATAGTGA TCTGAGTCTA CTTGCTCTTC CACCTTCTAG TCCCCAATTT
1141 AATATTGGAT TTCCTT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl