RDB16800 - pCAGGS-FLAG Qki7

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCAGGS-FLAG Qki7 (#RDB16800)

Expression vector of mouse K homology type RNA binding domain, quaking 7 (Qki7).

Clone info.	Expression vector of mouse K homology type RNA binding domain, quaking 7 (Qki7) tagged with FLAG at N-terminus, CAG promoter.
Comment	Expression was confirmed by the depositor with immunostaining.
Vector backbone	pCAGGS2 (plasmid)
Size of vector backbone	4.8 kb
Selectable markers	Amp^r
Gene/insert name	mouse Qk cDNA
Depositor\|Developer	Yano, Yoshika \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hayakawa-Yano, Y et al. Genes Dev. 31 (18), 1910-1925, 2017) and an acknowledgment to the DEPOSITOR are requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hayakawa-Yano, Y et al. Genes Dev. 31 (18), 1910-1925, 2017) and an acknowledgment to the DEPOSITOR are requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hayakawa-Yano, Y et al. Genes Dev. 31 (18), 1910-1925, 2017) を引用し、謝辞の表明を必要とする。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hayakawa-Yano, Y et al. Genes Dev. 31 (18), 1910-1925, 2017) を引用し、謝辞の表明を必要とする。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16800	pCAGGS-FLAG Qki7	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS-FLAG Qki7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16800).

Reference section:

Hayakawa-Yano, Y., Suyama, S., Nogami, M., Yugami, M., Koya, I., Furukawa, T., Zhou, L., Abe, M., Sakimura, K., Takebayashi, H., Nakanishi, A., Okano, H., Yano, M., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16800_A8L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F () Sequence file: RDB16800_A8L7a.seq
>D05707A3_A8L7_2_pCAG-F_H02_23_ABI24.ab1 1 AGGGGGGGGG GAAGGCTGCC TTCGGGGGGG GAGGGGCAGG GCGGGGTTCG GCTTCTGGCG 61 TGTGACCGGC GGCTCTAGCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC 121 TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTGC 181 TCGAGATCCA AGAGAGATAC CACCATGGCC CTCGAGGACT ACAAGGACGA CGATGACAAG 241 TCTAGAATGG TCGGGGAAAT GGAAACGAAG GAGAAGCCGA AGCCCACCCC AGATTATTTG 301 ATGCAGCTGA TGAACGACAA GAAGCTCATG AGCAGCCTGC CCAACTTCTG CGGGATCTTC 361 AACCACCTCG AGCGGCTGCT GGACGAAGAA ATTAGCAGAG TACGGAAAGA CATGTACAAT 421 GACACGTTAA ATGGCAGTAC AGAGAAAAGA AGTGCAGAAT TGCCTGACGC GGTGGGACCC 481 ATTGTTCAGT TACAAGAGAA ACTTTATGTG CCTGTAAAAG AATACCCTGA TTTTAATTTT 541 GTTGGGAGAA TCCTTGGACC TAGAGGACTT ACAGCTAAAC AACTTGAAGC AGAAACGGGA 601 TGTAAAATAA TGGTCCGAGG CAAAGGCTCA ATGAGGGATA AAAAGAAGGA GGAGCAAAAT 661 AGAGGCAAGC CCAATTGGGA GCATCTAAAT GAAGACTTAC ATGTACTAAT CACTGTGGAA 721 GATGCTCAGA ACAGAGCAGA AATCAAGCTG AAGAGAGCGG TTGAAGAAGT GAAGAAGTTA 781 CTGGTACCTG CGGCTGAAGG TGAAGACAGC CTGAAGAAGA TGCAGCTGAT GGAGCTTGCA 841 AATTCTGAAT GGCACCTACA GAGACGCCAA CATTAAATCA CCAGCCCTTG CCTTTTCTCT 901 TGCAGCAACT GCCCAGGCTG CTTCCAAGGA TCATCACTGG GGCCTGGCGG CCTGTCCTCC 961 CACCAAGCTG CTCCTGCGTA ACAACCTAAC GCCAGCCTGG GCCCTACCCA TAATGCCCTT 1021 TTGAATCAGA ACAAATAACA GAACCGCCTG GTCATTGCCC AAACGGGAAC TCCTTCAACT 1081 CAACTGGCTG GGCATAGGTT CCCCTTCCAA GGGCCCTTGA AGCCTGGGGT TAAATCTAAC 1141 ACAACCCCCT AAGTGAAATA ACCCCCCGTA CAACATTGGG GCACTCAGGC CTTCCAACCA 1201 AATCCGTTGT GAGAGTTATC CCGCGTATAT ATGTGTTGAG CAA //
Primer: pCA-bgter () Sequence file: RDB16800_A8L7b.seq
>D05707A3_A8L7_2_pCA-bgter_A03_03_ABI24.ab1 1 ACCCCAAGAA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG GTATTTGTGA 61 GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG 121 AATTCTCAAT GGGCTGAAAT ATCAGGCATG ACTGGCATTT CAATCCACTC TAACACACCA 181 CTGGGTTCAA TAGGGTACTC AAGGATTGAT GTAGCTGGTG CCAATGTGTA GGGGTATTCA 241 TAGGGTGTGT AGATTAACCC AGCTTCAGGC CCTGGAGGGA CTATTGCAGC AGTTGGGTGA 301 GGAGTTCCGT TTGGCATGAC AGCGGTCTGT ATTTGTCTGA TCAAAGGCAT TATGGTAGGG 361 CCAGCTGGCG TAGGTGTACG CAGAGCAGCT GGTGGGAGGA CAGGCGCAGG CCCAGTGATG 421 ATCCTTGGAG CAGCCTGGGC AGTTGCTGCA AGAGAAAAGG CAAGGGCTGG TGATTTAATG 481 TTGGCGTCTC TGTAGGTGCC ATTCAGAATT GCAAGCTCCA TCAGCTGCAT CTTCTTCAGG 541 CTGTCTTCAC CTTCAGCCGC AGGTACCAGT AACTTCTTCA CTTCTTCAAC CGCTCTCTTC 601 AGCTTGATTT CTGCTCTGTT CTGAGCATCT TCCACAGTGA TTAGTACATG TAAGTCTTCA 661 TTTAGATGCT CCCAATTGGG CTTGCCTCTA TTTTGCTCCT CCTTCTTTTT ATCCCTCATT 721 GAGCCTTTGC CTCGGACCAT TATTTTACAT CCCGTTTCTG CTTCAAGTTG TTTAGCTGTA 781 AGTCCTCTAG GTCCAAGGAT TCTCCCAACA AAATTAAAAT CAGGGTATTC TTTTACAGGC 841 ACATAAAGTT TCTCTTGTAA CTGAACAATG GGTCCCACCG CGTCAGGCAA TTCTGCACTT 901 CTTTTCTCTG TACTGCCATT TAACGTGTCA TTGTACATGT CTTTCCGTAC TCTGCTAATT 961 TCTTCGTCCA GCAGCCGCTC GAGGTGGTTT GAAGATCCCG CAGAAGTTGG GCAGGCTGCT 1021 CATGAGCTTT CTTGTCGTTC ATCAGCTGCA TCAATAATCT GGGGGTGGGC CTTCGGCCTC 1081 TCGTTCGGTT TCATTTCCCC GACATTCCTA GACTGTCAAT CGATCGATCC TTGGTAGTCC 1141 TGAGCGATGT GGTAATCGTC CTA //
Primer: SV40pro_F () Sequence file: RDB16800_A8L7c.seq
>D05707A3_A8L7_2_SV40pro_F_C01_03_ABI08.ab1 1 CCCGGGGCGC CGTTAGCCAA TTCAGGAAGT AGTGGAGGAG GCTTTTTTGG AGGCCTAGGC 61 TTTTGCAAAA AGCTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 121 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 181 TCATCAATGT ATCTTATCAT GTCTGGATCC GCTGCATTAA TGAATCGGCC AACGCGCGGG 241 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 301 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 361 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 421 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 481 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 541 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 601 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 661 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 721 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 781 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 841 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 901 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 961 GCAAACAAAC CACCGCTGGT AGCGGGTGGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 1021 CAGAAAAAAA GGATCTCAAG AAGGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAAT 1081 GGGAACGGAA AAACTTCACG TTTAGGGATT TTTGGGTCAT GGAGATTTAT CCAAACGAAT 1141 CCTTCACCTA GAGATTCCTT TTTAATGAAA TGAAGGCTGT TAAGTCAGTC CTAAGAAGGT 1201 GATATACTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Hayakawa-Yano, Y., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239. [link to RRC of NBRP]

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Other resources in our bank

mouse Qk

External Database
mouse Qk

Reference sequence