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pCAGGS-FLAG Qki7

Expression vector of mouse K homology type RNA binding domain, quaking 7 (Qki7).

Catalog number RDB16800
Resource name pCAGGS-FLAG Qki7
Clone info. Expression vector of mouse K homology type RNA binding domain, quaking 7 (Qki7) tagged with FLAG at N-terminus, CAG promoter.
Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS2 (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name mouse Qk cDNA
Depositor Yano, Yoshika |
 
Other clones in our bank mouse Qk (NCBI Gene 19317) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hayakawa-Yano, Y et al. Genes Dev. 31 (18), 1910-1925, 2017) and an acknowledgment to the DEPOSITOR are requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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RDB16800 pCAGGS-FLAG Qki7 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Hayakawa-Yano, Y., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16800_A8L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F RDB16800_A8L7a.seq
>D05707A3_A8L7_2_pCAG-F_H02_23_ABI24.ab1
    1 AGGGGGGGGG GAAGGCTGCC TTCGGGGGGG GAGGGGCAGG GCGGGGTTCG GCTTCTGGCG
   61 TGTGACCGGC GGCTCTAGCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC
  121 TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTGC
  181 TCGAGATCCA AGAGAGATAC CACCATGGCC CTCGAGGACT ACAAGGACGA CGATGACAAG
  241 TCTAGAATGG TCGGGGAAAT GGAAACGAAG GAGAAGCCGA AGCCCACCCC AGATTATTTG
  301 ATGCAGCTGA TGAACGACAA GAAGCTCATG AGCAGCCTGC CCAACTTCTG CGGGATCTTC
  361 AACCACCTCG AGCGGCTGCT GGACGAAGAA ATTAGCAGAG TACGGAAAGA CATGTACAAT
  421 GACACGTTAA ATGGCAGTAC AGAGAAAAGA AGTGCAGAAT TGCCTGACGC GGTGGGACCC
  481 ATTGTTCAGT TACAAGAGAA ACTTTATGTG CCTGTAAAAG AATACCCTGA TTTTAATTTT
  541 GTTGGGAGAA TCCTTGGACC TAGAGGACTT ACAGCTAAAC AACTTGAAGC AGAAACGGGA
  601 TGTAAAATAA TGGTCCGAGG CAAAGGCTCA ATGAGGGATA AAAAGAAGGA GGAGCAAAAT
  661 AGAGGCAAGC CCAATTGGGA GCATCTAAAT GAAGACTTAC ATGTACTAAT CACTGTGGAA
  721 GATGCTCAGA ACAGAGCAGA AATCAAGCTG AAGAGAGCGG TTGAAGAAGT GAAGAAGTTA
  781 CTGGTACCTG CGGCTGAAGG TGAAGACAGC CTGAAGAAGA TGCAGCTGAT GGAGCTTGCA
  841 AATTCTGAAT GGCACCTACA GAGACGCCAA CATTAAATCA CCAGCCCTTG CCTTTTCTCT
  901 TGCAGCAACT GCCCAGGCTG CTTCCAAGGA TCATCACTGG GGCCTGGCGG CCTGTCCTCC
  961 CACCAAGCTG CTCCTGCGTA ACAACCTAAC GCCAGCCTGG GCCCTACCCA TAATGCCCTT
 1021 TTGAATCAGA ACAAATAACA GAACCGCCTG GTCATTGCCC AAACGGGAAC TCCTTCAACT
 1081 CAACTGGCTG GGCATAGGTT CCCCTTCCAA GGGCCCTTGA AGCCTGGGGT TAAATCTAAC
 1141 ACAACCCCCT AAGTGAAATA ACCCCCCGTA CAACATTGGG GCACTCAGGC CTTCCAACCA
1201 AATCCGTTGT GAGAGTTATC CCGCGTATAT ATGTGTTGAG CAA
//
Primer: pCA-bgter RDB16800_A8L7b.seq
>D05707A3_A8L7_2_pCA-bgter_A03_03_ABI24.ab1
    1 ACCCCAAGAA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG GTATTTGTGA
   61 GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG
  121 AATTCTCAAT GGGCTGAAAT ATCAGGCATG ACTGGCATTT CAATCCACTC TAACACACCA
  181 CTGGGTTCAA TAGGGTACTC AAGGATTGAT GTAGCTGGTG CCAATGTGTA GGGGTATTCA
  241 TAGGGTGTGT AGATTAACCC AGCTTCAGGC CCTGGAGGGA CTATTGCAGC AGTTGGGTGA
  301 GGAGTTCCGT TTGGCATGAC AGCGGTCTGT ATTTGTCTGA TCAAAGGCAT TATGGTAGGG
  361 CCAGCTGGCG TAGGTGTACG CAGAGCAGCT GGTGGGAGGA CAGGCGCAGG CCCAGTGATG
  421 ATCCTTGGAG CAGCCTGGGC AGTTGCTGCA AGAGAAAAGG CAAGGGCTGG TGATTTAATG
  481 TTGGCGTCTC TGTAGGTGCC ATTCAGAATT GCAAGCTCCA TCAGCTGCAT CTTCTTCAGG
  541 CTGTCTTCAC CTTCAGCCGC AGGTACCAGT AACTTCTTCA CTTCTTCAAC CGCTCTCTTC
  601 AGCTTGATTT CTGCTCTGTT CTGAGCATCT TCCACAGTGA TTAGTACATG TAAGTCTTCA
  661 TTTAGATGCT CCCAATTGGG CTTGCCTCTA TTTTGCTCCT CCTTCTTTTT ATCCCTCATT
  721 GAGCCTTTGC CTCGGACCAT TATTTTACAT CCCGTTTCTG CTTCAAGTTG TTTAGCTGTA
  781 AGTCCTCTAG GTCCAAGGAT TCTCCCAACA AAATTAAAAT CAGGGTATTC TTTTACAGGC
  841 ACATAAAGTT TCTCTTGTAA CTGAACAATG GGTCCCACCG CGTCAGGCAA TTCTGCACTT
  901 CTTTTCTCTG TACTGCCATT TAACGTGTCA TTGTACATGT CTTTCCGTAC TCTGCTAATT
  961 TCTTCGTCCA GCAGCCGCTC GAGGTGGTTT GAAGATCCCG CAGAAGTTGG GCAGGCTGCT
 1021 CATGAGCTTT CTTGTCGTTC ATCAGCTGCA TCAATAATCT GGGGGTGGGC CTTCGGCCTC
 1081 TCGTTCGGTT TCATTTCCCC GACATTCCTA GACTGTCAAT CGATCGATCC TTGGTAGTCC
1141 TGAGCGATGT GGTAATCGTC CTA
//
Primer: SV40pro_F RDB16800_A8L7c.seq
>D05707A3_A8L7_2_SV40pro_F_C01_03_ABI08.ab1
    1 CCCGGGGCGC CGTTAGCCAA TTCAGGAAGT AGTGGAGGAG GCTTTTTTGG AGGCCTAGGC
   61 TTTTGCAAAA AGCTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA
  121 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC
  181 TCATCAATGT ATCTTATCAT GTCTGGATCC GCTGCATTAA TGAATCGGCC AACGCGCGGG
  241 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
  301 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
  361 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
  421 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
  481 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
  541 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
  601 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
  661 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
  721 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
  781 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
  841 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
  901 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
  961 GCAAACAAAC CACCGCTGGT AGCGGGTGGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
 1021 CAGAAAAAAA GGATCTCAAG AAGGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAAT
 1081 GGGAACGGAA AAACTTCACG TTTAGGGATT TTTGGGTCAT GGAGATTTAT CCAAACGAAT
 1141 CCTTCACCTA GAGATTCCTT TTTAATGAAA TGAAGGCTGT TAAGTCAGTC CTAAGAAGGT
1201 GATATACTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hayakawa-Yano, Y., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239.

2019.06.25

GNP_filter3_RDBDEP_html_190430.pl