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pCAGGS-FLAG Qki6

Expression vector of mouse K homology type RNA binding domain, quaking 6 (Qki6).

Catalog number RDB16799
Resource name pCAGGS-FLAG Qki6
Clone info. Expression vector of mouse K homology type RNA binding domain, quaking 6 (Qki6) tagged with FLAG at N-terminus, CAG promoter.
Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS2 (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name mouse Qk cDNA
Depositor Yano, Yoshika |
 
Other clones in our bank mouse Qk (NCBI Gene 19317) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hayakawa-Yano, Y et al. Genes Dev. 31 (18), 1910-1925, 2017) and an acknowledgment to the DEPOSITOR are requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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RDB16799 pCAGGS-FLAG Qki6 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Hayakawa-Yano, Y., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16799_A8L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F RDB16799_A8L7a.seq
>D05707A2_A8L7_3_pCAG-F_F02_17_ABI24.ab1
    1 GTATGGGGGG GCAGCCTGCC TTCGGGGGGG AGGGGCAGGG CGGGGTTCGG CTTCTGGCGT
   61 GTGACCGGCG GCTCTAGCTA GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT
  121 ACAGCTCCTG GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGCT
  181 CGAGATCCAA GAGAGATACC ACCATGGCCC TCGAGGACTA CAAGGACGAC GATGACAAGT
  241 CTAGAATGGT CGGGGAAATG GAAACGAAGG AGAAGCCGAA GCCCACCCCA GATTATTTGA
  301 TGCAGCTGAT GAACGACAAG AAGCTCATGA GCAGCCTGCC CAACTTCTGC GGGATCTTCA
  361 ACCACCTCGA GCGGCTGCTG GACGAAGAAA TTAGCAGAGT ACGGAAAGAC ATGTACAATG
  421 ACACGTTAAA TGGCAGTACA GAGAAAAGAA GTGCAGAATT GCCTGACGCG GTGGGACCCA
  481 TTGTTCAGTT ACAAGAGAAA CTTTATGTGC CTGTAAAAGA ATACCCTGAT TTTAATTTTG
  541 TTGGGAGAAT CCTTGGACCT AGAGGACTTA CAGCTAAACA ACTTGAAGCA GAAACGGGAT
  601 GTAAAATAAT GGTCCGAGGC AAAGGCTCAA TGAGGGATAA AAAGAAGGAG GAGCAAAATA
  661 GAGGCAAGCC CAATTGGGAG CATCTAAATG AAGACTTACA TGTACTAATC ACTGTGGAAG
  721 ATGCTCAGAA CAGAGCAGAA ATCAAGCTGA AGAGAGCGGT TGAAGAAGTG AAGAAGTTAC
  781 TGGTACCTGC GGCTGAAGGT GAAGACAGCC TGAAGAAGAT GCAGCTGATG GAGCTTGCAA
  841 TTCTGAATGG CACCTACAGA GACGCCAACA TTAAATCACC AGCCCTTGCC TTTTCTCTTG
  901 CAGCAACTGC CCAGGCTGCT CCAAGGATCA TCACTGGGCC TGCGCCTGTC CTCCCACCAG
  961 CTGCTCTGCG TACACCTACG CCAGCTGGGC CCTACCATAA TGCCTTTGAT CAGACAAATA
 1021 CAGACCGCTG TCATGCCCAA ACGGGAACTC CTCACCCAAC TGCTGCAATA GTCCTCAGGG
 1081 CCCTGAAGCT GATTAATCTA CCAACCTATG AATAACCCGT ACACATTGGG CACCAGCCTA
1141 TCAAATTCCA AATC
//
Primer: pCA-bgter RDB16799_A8L7b.seq
>D05707A2_A8L7_3_pCA-bgter_G02_20_ABI24.ab1
    1 AGCCAGAAGT CCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT TTGTGAGCCA
   61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGAATT
  121 CTTAGCCTTT CGTTGGGAAA GCCATACCTA ACACACCACT GGGTTCAATA GGGTACTCAA
  181 GGATTGATGT AGCTGGTGCC AATGTGTAGG GGTATTCATA GGGTGTGTAG ATTAACCCAG
  241 CTTCAGGCCC TGGAGGGACT ATTGCAGCAG TTGGGTGAGG AGTTCCGTTT GGCATGACAG
  301 CGGTCTGTAT TTGTCTGATC AAAGGCATTA TGGTAGGGCC AGCTGGCGTA GGTGTACGCA
  361 GAGCAGCTGG TGGGAGGACA GGCGCAGGCC CAGTGATGAT CCTTGGAGCA GCCTGGGCAG
  421 TTGCTGCAAG AGAAAAGGCA AGGGCTGGTG ATTTAATGTT GGCGTCTCTG TAGGTGCCAT
  481 TCAGAATTGC AAGCTCCATC AGCTGCATCT TCTTCAGGCT GTCTTCACCT TCAGCCGCAG
  541 GTACCAGTAA CTTCTTCACT TCTTCAACCG CTCTCTTCAG CTTGATTTCT GCTCTGTTCT
  601 GAGCATCTTC CACAGTGATT AGTACATGTA AGTCTTCATT TAGATGCTCC CAATTGGGCT
  661 TGCCTCTATT TTGCTCCTCC TTCTTTTTAT CCCTCATTGA GCCTTTGCCT CGGACCATTA
  721 TTTTACATCC CGTTTCTGCT TCAAGTTGTT TAGCTGTAAG TCCTCTAGGT CCAAGGATTC
  781 TCCCAACAAA ATTAAAATCA GGGTATTCTT TTACAGGCAC ATAAAGTTTC TCTTGTAACT
  841 GAACAATGGG TCCCACCGCG TCAGGCAATT CTGCACTTCT TTTCTCTGTA CTGCCATTTA
  901 ACGTGTCATT GTACATGTCT TTCCGTACTC TGCTAATTTC TTCGTCCAGC AGCCGCTCGA
  961 GGTGGTTGAA GATCCCGCAG AAGTTGGGCA GGCTGCTCAT GAGCTTCTTT GTCGTTCATC
 1021 AGCTGCATCA AAATAAATCT GGGGGTGGGC TTTCGGCTTT CTCCTTTCGA TTTTCATTTC
 1081 CCCGACCCAT TTCTAGACTT GGTCATTCGG TTCGTTCTTG GTAGTCTCGA AGGGCCAATT
1141 GGGGTGTGTT AATTCTC
//
Primer: SV40pro_F RDB16799_A8L7c.seq
>D05707A2_A8L7_3_SV40pro_F_B01_02_ABI08.ab1
    1 CCTGGGGGCC CGTTAAACTT ATTCCCGGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG
   61 CTTTTGCAAA AAGCTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
  121 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
  181 CTCATCAATG TATCTTATCA TGTCTGGATC CGCTGCATTA ATGAATCGGC CAACGCGCGG
  241 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
  301 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
  361 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
  421 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
  481 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
  541 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
  601 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
  661 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
  721 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
  781 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
  841 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA AACAGTATTT
  901 GGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTTCGGAAA AAGAGTTGGT AGCTCTTGAT
  961 CCGGCAAACA AACACCGCTG GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGG
 1021 CGCAAGAAAA AAAAGATCTC AAGAAGAATC CTTTTGATCT TTTTCTACGG GGGGTCTGAC
 1081 GCTCAGTGGA ACGAAAACTC ACGGTTAAGG GAATTTGGTC CATGGAGAAT TATCAAAAGG
 1141 AATCTCACCT AGAATTCTTT TACATAAAAA TCAGTTTAAA TCCAATCTAA AGTTGATTAA
1201 TCATCCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hayakawa-Yano, Y., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239.

2019.06.25

GNP_filter3_RDBDEP_html_190430.pl