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pCAGGS-FLAG Qki5

Expression vector of mouse K homology type RNA binding domain, quaking 5 (Qki5).

Catalog number RDB16798
Resource name pCAGGS-FLAG Qki5
Clone info. Expression vector of mouse K homology type RNA binding domain, quaking 5 (Qki5) tagged with FLAG at N-terminus, CAG promoter.
Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS2 (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name mouse Qk cDNA
Depositor Yano, Yoshika |
 
Other clones in our bank mouse Qk (NCBI Gene 19317) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hayakawa-Yano, Y et al. Genes Dev. 31 (18), 1910-1925, 2017) and an acknowledgment to the DEPOSITOR are requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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RDB16798 pCAGGS-FLAG Qki5 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Hayakawa-Yano, Y., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16798_A8L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F RDB16798_A8L7a.seq
>D05707A1_A8L7_2_pCAG-F_D02_11_ABI24.ab1
    1 GCAGAGGGGG GCGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT
   61 GTGACCGGCG GCTCTAGCTA GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT
  121 ACAGCTCCTG GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGCT
  181 CGAGATCCAA GAGAGATACC ACCATGGCCC TCGAGGACTA CAAGGACGAC GATGACAAGT
  241 CTAGAATGGT CGGGGAAATG GAAACGAAGG AGAAGCCGAA GCCCACCCCA GATTATTTGA
  301 TGCAGCTGAT GAACGACAAG AAGCTCATGA GCAGCCTGCC CAACTTCTGC GGGATCTTCA
  361 ACCACCTCGA GCGGCTGCTG GACGAAGAAA TTAGCAGAGT ACGGAAAGAC ATGTACAATG
  421 ACACGTTAAA TGGCAGTACA GAGAAAAGAA GTGCAGAATT GCCTGACGCG GTGGGACCCA
  481 TTGTTCAGTT ACAAGAGAAA CTTTATGTGC CTGTAAAAGA ATACCCTGAT TTTAATTTTG
  541 TTGGGAGAAT CCTTGGACCT AGAGGACTTA CAGCTAAACA ACTTGAAGCA GAAACGGGAT
  601 GTAAAATAAT GGTCCGAGGC AAAGGCTCAA TGAGGGATAA AAAGAAGGAG GAGCAAAATA
  661 GAGGCAAGCC CAATTGGGAG CATCTAAATG AAGACTTACA TGTACTAATC ACTGTGGAAG
  721 ATGCTCAGAA CAGAGCAGAA ATCAAGCTGA AGAGAGCGGT TGAAGAAGTG AAGAAGTTAC
  781 TGGTACCTGC GGCTGAAGGT GAAGACAGCC TGAAGAAGAT GCAGCTGATG GAGCTTGCAA
  841 TTCTGAATGG CACCTACAGA GACGCCAACA TTAAATCACC AGCCCTTGCC TTTTCTCTTG
  901 CAGCAACTGC CCAGGCTGCT CCAAAGGATC ATCACTGGGC CTGCGCCTGT CCTCCCACCA
  961 GCTGCTCTGC GTACACCTAC GCCAGCTGGC CCTACCATAA TGCCTTTGAT CAGACAATAC
 1021 AGACCGCTGT CATGCCCAAA CGGAACTCCT CACCCAACTG CTGCAATTAG TCCCTCAGGG
 1081 CCTGAAGCTG GGTTTAATCC TACACACCCT ATGGATACCC TTAACAACAT TGGCATCAAG
1141 CCCTTAACAT TCCAAAATTC
//
Primer: pCA-bgter RDB16798_A8L7b.seq
>D05707A1_A8L7_2_pCA-bgter_E02_14_ABI24.ab1
    1 CCCGCAATTT AGGTCCCAAT AAATTTTTGG CAGAGGGAAA AAGATCTCAG TGGTATTTGT
   61 GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG
  121 TGAATTCTTA GTTGCCGGTG GCGGCTCGGT CTGCGGTCAC AATCCTTTGG TAAGGATGGA
  181 CACGCATATC GTGCCTTCGA ACTTTAGTAG CCACCGCACC TAACACACCA CTGGGTTCAA
  241 TAGGGTACTC AAGGATTGAT GTAGCTGGTG CCAATGTGTA GGGGTATTCA TAGGGTGTGT
  301 AGATTAACCC AGCTTCAGGC CCTGGAGGGA CTATTGCAGC AGTTGGGTGA GGAGTTCCGT
  361 TTGGCATGAC AGCGGTCTGT ATTTGTCTGA TCAAAGGCAT TATGGTAGGG CCAGCTGGCG
  421 TAGGTGTACG CAGAGCAGCT GGTGGGAGGA CAGGCGCAGG CCCAGTGATG ATCCTTGGAG
  481 CAGCCTGGGC AGTTGCTGCA AGAGAAAAGG CAAGGGCTGG TGATTTAATG TTGGCGTCTC
  541 TGTAGGTGCC ATTCAGAATT GCAAGCTCCA TCAGCTGCAT CTTCTTCAGG CTGTCTTCAC
  601 CTTCAGCCGC AGGTACCAGT AACTTCTTCA CTTCTTCAAC CGCTCTCTTC AGCTTGATTT
  661 CTGCTCTGTT CTGAGCATCT TCCACAGTGA TTAGTACATG TAAGTCTTCA TTTAGATGCT
  721 CCCAATTGGG CTTGCCTCTA TTTTGCTCCT CCTTCTTTTT ATCCCTCATT GAGCCTTTGC
  781 CTCGGACCAT TATTTTACAT CCCGTTTCTG CTTCAAGTTG TTTAGCTGTA AGTCCTCTAG
  841 GTCCAAGGAT TCTCCCAACA AAATTAAAAT CAGGGTATTC TTTTACAGGC ACATAAAGTT
  901 TCTCTTGTAA CTGAACAATG GGGTCCCACC GCGTCAGGCA ATTTCTGCAC TTCTTTTCTC
  961 TGTACTGCCA TTTAACGTGT CATTGTACAT GTCTTTCCGT ACTCTGCTAA TTTCTTCGTC
 1021 CAGCAGCCCG CTCGAGGTGG TTGAAGATCC GCAGAAGTTG GCAGGCTGCT CATGAGCCTT
 1081 CCTTGTCGGT TCATCAGCTG CAATCAAATA TCTGGGATGG ACCTTCCGCT TTCTTCCTTC
1141 GATTTTCCAA TTTTC
//
Primer: SV40pro_F RDB16798_A8L7c.seq
>D05707A1_A8L7_2_SV40pro_F_A01_01_ABI08.ab1
    1 CCTTTGGGCC GTTAAGCCTT TCAGAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
  121 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT
  181 CAATGTATCT TATCATGTCT GGATCCGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
  241 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
  301 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
  361 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
  421 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
  481 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
  541 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
  601 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
  661 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
  721 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
  781 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
  841 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
  901 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
  961 CAAACCACCG CTGGTAGCGG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
 1021 AAAAGGATCT CAAGAAGATC CTTTTGATCT TTTTCTACGG GGTCTGACGC TCAGTGGAAC
 1081 GAAACCTCAC GTTAAAGGAT TTCGTCATGA GGATATCAAA AGGATCCTCA CTTAGATCCT
1141 TTAATAAAAT GAAGTTTAAT TCACTCTAAA GGTAAATAAT ATGAGAGTTA AAACCTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hayakawa-Yano, Y., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239.

2019.06.25

GNP_filter3_RDBDEP_html_190430.pl