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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS-FLAG Qki5 (#RDB16798)

Expression vector of mouse K homology type RNA binding domain, quaking 5 (Qki5).

Clone info. Expression vector of mouse K homology type RNA binding domain, quaking 5 (Qki5) tagged with FLAG at N-terminus, CAG promoter.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS2 (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name mouse Qk cDNA
Depositor|Developer Yano, Yoshika |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hayakawa-Yano, Y et al. Genes Dev. 31 (18), 1910-1925, 2017) and an acknowledgment to the DEPOSITOR are requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hayakawa-Yano, Y et al. Genes Dev. 31 (18), 1910-1925, 2017) を引用し、謝辞の表明を必要とする。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16798 pCAGGS-FLAG Qki5 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS-FLAG Qki5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16798).

Reference section:

Hayakawa-Yano, Y., Suyama, S., Nogami, M., Yugami, M., Koya, I., Furukawa, T., Zhou, L., Abe, M., Sakimura, K., Takebayashi, H., Nakanishi, A., Okano, H., Yano, M., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16798_A8L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F ()
Sequence file: RDB16798_A8L7a.seq check
>D05707A1_A8L7_2_pCAG-F_D02_11_ABI24.ab1
    1 GCAGAGGGGG GCGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT
   61 GTGACCGGCG GCTCTAGCTA GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT
  121 ACAGCTCCTG GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGCT
  181 CGAGATCCAA GAGAGATACC ACCATGGCCC TCGAGGACTA CAAGGACGAC GATGACAAGT
  241 CTAGAATGGT CGGGGAAATG GAAACGAAGG AGAAGCCGAA GCCCACCCCA GATTATTTGA
  301 TGCAGCTGAT GAACGACAAG AAGCTCATGA GCAGCCTGCC CAACTTCTGC GGGATCTTCA
  361 ACCACCTCGA GCGGCTGCTG GACGAAGAAA TTAGCAGAGT ACGGAAAGAC ATGTACAATG
  421 ACACGTTAAA TGGCAGTACA GAGAAAAGAA GTGCAGAATT GCCTGACGCG GTGGGACCCA
  481 TTGTTCAGTT ACAAGAGAAA CTTTATGTGC CTGTAAAAGA ATACCCTGAT TTTAATTTTG
  541 TTGGGAGAAT CCTTGGACCT AGAGGACTTA CAGCTAAACA ACTTGAAGCA GAAACGGGAT
  601 GTAAAATAAT GGTCCGAGGC AAAGGCTCAA TGAGGGATAA AAAGAAGGAG GAGCAAAATA
  661 GAGGCAAGCC CAATTGGGAG CATCTAAATG AAGACTTACA TGTACTAATC ACTGTGGAAG
  721 ATGCTCAGAA CAGAGCAGAA ATCAAGCTGA AGAGAGCGGT TGAAGAAGTG AAGAAGTTAC
  781 TGGTACCTGC GGCTGAAGGT GAAGACAGCC TGAAGAAGAT GCAGCTGATG GAGCTTGCAA
  841 TTCTGAATGG CACCTACAGA GACGCCAACA TTAAATCACC AGCCCTTGCC TTTTCTCTTG
  901 CAGCAACTGC CCAGGCTGCT CCAAAGGATC ATCACTGGGC CTGCGCCTGT CCTCCCACCA
  961 GCTGCTCTGC GTACACCTAC GCCAGCTGGC CCTACCATAA TGCCTTTGAT CAGACAATAC
 1021 AGACCGCTGT CATGCCCAAA CGGAACTCCT CACCCAACTG CTGCAATTAG TCCCTCAGGG
 1081 CCTGAAGCTG GGTTTAATCC TACACACCCT ATGGATACCC TTAACAACAT TGGCATCAAG
1141 CCCTTAACAT TCCAAAATTC
//
Primer: pCA-bgter ()
Sequence file: RDB16798_A8L7b.seq check
>D05707A1_A8L7_2_pCA-bgter_E02_14_ABI24.ab1
    1 CCCGCAATTT AGGTCCCAAT AAATTTTTGG CAGAGGGAAA AAGATCTCAG TGGTATTTGT
   61 GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG
  121 TGAATTCTTA GTTGCCGGTG GCGGCTCGGT CTGCGGTCAC AATCCTTTGG TAAGGATGGA
  181 CACGCATATC GTGCCTTCGA ACTTTAGTAG CCACCGCACC TAACACACCA CTGGGTTCAA
  241 TAGGGTACTC AAGGATTGAT GTAGCTGGTG CCAATGTGTA GGGGTATTCA TAGGGTGTGT
  301 AGATTAACCC AGCTTCAGGC CCTGGAGGGA CTATTGCAGC AGTTGGGTGA GGAGTTCCGT
  361 TTGGCATGAC AGCGGTCTGT ATTTGTCTGA TCAAAGGCAT TATGGTAGGG CCAGCTGGCG
  421 TAGGTGTACG CAGAGCAGCT GGTGGGAGGA CAGGCGCAGG CCCAGTGATG ATCCTTGGAG
  481 CAGCCTGGGC AGTTGCTGCA AGAGAAAAGG CAAGGGCTGG TGATTTAATG TTGGCGTCTC
  541 TGTAGGTGCC ATTCAGAATT GCAAGCTCCA TCAGCTGCAT CTTCTTCAGG CTGTCTTCAC
  601 CTTCAGCCGC AGGTACCAGT AACTTCTTCA CTTCTTCAAC CGCTCTCTTC AGCTTGATTT
  661 CTGCTCTGTT CTGAGCATCT TCCACAGTGA TTAGTACATG TAAGTCTTCA TTTAGATGCT
  721 CCCAATTGGG CTTGCCTCTA TTTTGCTCCT CCTTCTTTTT ATCCCTCATT GAGCCTTTGC
  781 CTCGGACCAT TATTTTACAT CCCGTTTCTG CTTCAAGTTG TTTAGCTGTA AGTCCTCTAG
  841 GTCCAAGGAT TCTCCCAACA AAATTAAAAT CAGGGTATTC TTTTACAGGC ACATAAAGTT
  901 TCTCTTGTAA CTGAACAATG GGGTCCCACC GCGTCAGGCA ATTTCTGCAC TTCTTTTCTC
  961 TGTACTGCCA TTTAACGTGT CATTGTACAT GTCTTTCCGT ACTCTGCTAA TTTCTTCGTC
 1021 CAGCAGCCCG CTCGAGGTGG TTGAAGATCC GCAGAAGTTG GCAGGCTGCT CATGAGCCTT
 1081 CCTTGTCGGT TCATCAGCTG CAATCAAATA TCTGGGATGG ACCTTCCGCT TTCTTCCTTC
1141 GATTTTCCAA TTTTC
//
Primer: SV40pro_F ()
Sequence file: RDB16798_A8L7c.seq check
>D05707A1_A8L7_2_SV40pro_F_A01_01_ABI08.ab1
    1 CCTTTGGGCC GTTAAGCCTT TCAGAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
  121 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT
  181 CAATGTATCT TATCATGTCT GGATCCGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
  241 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
  301 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
  361 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
  421 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
  481 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
  541 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
  601 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
  661 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
  721 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
  781 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
  841 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
  901 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
  961 CAAACCACCG CTGGTAGCGG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
 1021 AAAAGGATCT CAAGAAGATC CTTTTGATCT TTTTCTACGG GGTCTGACGC TCAGTGGAAC
 1081 GAAACCTCAC GTTAAAGGAT TTCGTCATGA GGATATCAAA AGGATCCTCA CTTAGATCCT
1141 TTAATAAAAT GAAGTTTAAT TCACTCTAAA GGTAAATAAT ATGAGAGTTA AAACCTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hayakawa-Yano, Y., An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes Dev. 31 (18): 1910-1925 (2017). PMID 29021239. [link to RRC of NBRP]