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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA-flag-humanPLCz-AID-EGFP-pA

Expression vector of human phospholipase C zeta (PLC zeta) tagged with mAID degron.

Catalog number RDB16761
Resource name pcDNA-flag-humanPLCz-AID-EGFP-pA
Clone info. Expression vector of human phospholipase C zeta (PLC zeta) mRNA synthesis, tagged with FLAG at N-terminus and AID-EGFP cassette at C-terminus. Auxin-inducible degron (AID) system clone for in vitro transcription, which has an extended poly (A) sequence.
Comment Expression was confirmed by the depositor with EGFP monitoring by microinjection of its mRNA into mouse oocytes.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human PLCz cDNA Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Ogura, Atsuo |
Other clones in our bank
External Database
Human PLCz Arabidopsis thaliana AtIAA17
        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sato, K. et al. Anim. Sci. J. 84 (4): 359-368, 2013; Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) and an acknowledgment to the DEPOSITOR (Dr. Junya Ito, Azabu University, Dr. Atsuo Ogura, RIKEN BRC) are requested.
        Additional terms and conditions:
        In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
        Ordering Please visit Information of Request for Distribution.[link] 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Sato, K. et al. Anim. Sci. J. 84 (4): 359-368, 2013; Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018） を引用し、寄託者 (麻布大学　伊藤潤哉、RIKEN BRC 小倉淳郎) への謝辞の表明を必要とする。
        付加的提供条件：
        利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB16761 pcDNA-flag-humanPLCz-AID-EGFP-pA DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The pcDNA-flag-humanPLCz-AID-EGFP-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16761).

        Reference section:

        Miura, K., Matoba, S., Ogonuki, N., Namiki, T., Ito, J., Kashiwazaki, N., Ogura, A., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504.

        Further references such as user reports and related articles (go to bottom)

        References and tips

        Featured content

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB16761_A8K6p1-3.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward (Pr0016)
        Region: CMV pro,FLAG,insert 5'(hPLCZ1 5')
        Sequence file: RDB16761_A8K6e.seq check
        >D06043A1_A8K6_1_CMV-Forward_A04_01_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNCNN NTGNNGCAAA NGGGCGGTAN GCGTGTACGG TGGGAGGTCT
           61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
          121 ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGTTAAG CTTATGGACT ACAAAGACGA
          181 TGACGACAAG GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCACG AAATGAGATG
          241 GTTTTTGTCA AAGATTCAGG ATGACTTCAG AGGTGGAAAA ATTAACCTAG AAAAAACTCA
          301 GAGGTTACTT GAAAAATTAG ATATTCGGTG CAGTTATATT CATGTGAAAC AGATTTTTAA
          361 GGACAATGAC AGGCTGAAAC AAGGAAGAAT CACCATAGAA GAATTTAGAG CAATTTATCG
          421 AATTATCACG CACAGAGAAG AAATTATTGA GATTTTCAAC ACATATTCTG AAAACCGGAA
          481 AATTCTTTTA GCAAGTAATC TGGCTCAATT TCTGACACAA GAACAATATG CAGCTGAGAT
          541 GAGTAAAGCT ATTGCTTTTG AGATCATTCA GAAATACGAG CCTATCGAAG AAGTTAGGAA
          601 AGCACACCAA ATGTCATTAG AAGGTTTTAC AAGATACATG GATTCACGTG AATGTCTACT
          661 GTTTAAAAAT GAATGTAGAA AAGTTTATCA AGATATGACT CATCCATTAA ATGATTATTT
          721 TATTTCATCT TCACATAACA CATATTTGGT ATCTGATCAA TTATTGNGAC CAAGTGANCT
          781 TTGGGGATAT GTAAGTGCCC TTGTGAAAGG ATGCCGTTGT TTGGAGATTG ACTGCTGGGA
          841 TGGAGCACAA AATGAACCTG TTGTATATCA TGGCTACNCA CTCACAAGCA AACTTCTGTT
          901 NNAAACTGNT ATCCNAGCTA TACACNNNNA TGCANNCATG ANNTCTGANN ACCCNANNGG
        961 NGNTCNNNTT ANNAAANCAC TGNNNNCNNN NNNNNNN
        //
        Primer: GNP Primer #10 (Pr0763)
        Region: TRF2-3'UTR + polyA83,insert 3'(EGFP 3')
        Sequence file: RDB16761_A8K6f.seq check
        >D06043A1_A8K6_1_GNP_Primer#10_C04_07_ABI24.ab1
            1 NNNNNNNNNN NNNTNATTTN NNNGNNAACG TTTACNGGTT CTTTGGTGCC CATAGTCGAG
           61 CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCTTATTAC TTGTACAGCT CGTCCATGCC
          121 GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT
          181 GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG
          241 GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC
          301 GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT
          361 ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC
          421 CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC
          481 CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA
          541 GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT
          601 GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT
          661 GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAN GTGGCATCGC CCTCGCCCTC
          721 GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC
          781 CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACTTTATAC NTCCTCAAAT CGANTTTCCT
          841 CAAGTACGGN GCTCCGTCCN TTGATACCTT CACGAACGCC GCCGCCTCCN GGGCCACNGC
          901 TNGNTTTTTN NNANGAAACC NTCNNGTNCT TCCNGNATGA NCNCNNNNNN GGNNATCCNN
        961 NNNCNNNNNN NNNN
        //
        Primer: EGFP-N (Pr0027)
        Region: insert mid(AID 5',hPLCZ1 3')
        Sequence file: RDB16761_A8K6g.seq check
        >D06043A1_A8K6_1_CMV-Forward_A04_01_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNCNN NTGNNGCAAA NGGGCGGTAN GCGTGTACGG TGGGAGGTCT
           61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
          121 ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGTTAAG CTTATGGACT ACAAAGACGA
          181 TGACGACAAG GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCACG AAATGAGATG
          241 GTTTTTGTCA AAGATTCAGG ATGACTTCAG AGGTGGAAAA ATTAACCTAG AAAAAACTCA
          301 GAGGTTACTT GAAAAATTAG ATATTCGGTG CAGTTATATT CATGTGAAAC AGATTTTTAA
          361 GGACAATGAC AGGCTGAAAC AAGGAAGAAT CACCATAGAA GAATTTAGAG CAATTTATCG
          421 AATTATCACG CACAGAGAAG AAATTATTGA GATTTTCAAC ACATATTCTG AAAACCGGAA
          481 AATTCTTTTA GCAAGTAATC TGGCTCAATT TCTGACACAA GAACAATATG CAGCTGAGAT
          541 GAGTAAAGCT ATTGCTTTTG AGATCATTCA GAAATACGAG CCTATCGAAG AAGTTAGGAA
          601 AGCACACCAA ATGTCATTAG AAGGTTTTAC AAGATACATG GATTCACGTG AATGTCTACT
          661 GTTTAAAAAT GAATGTAGAA AAGTTTATCA AGATATGACT CATCCATTAA ATGATTATTT
          721 TATTTCATCT TCACATAACA CATATTTGGT ATCTGATCAA TTATTGNGAC CAAGTGANCT
          781 TTGGGGATAT GTAAGTGCCC TTGTGAAAGG ATGCCGTTGT TTGGAGATTG ACTGCTGGGA
          841 TGGAGCACAA AATGAACCTG TTGTATATCA TGGCTACNCA CTCACAAGCA AACTTCTGTT
          901 NNAAACTGNT ATCCNAGCTA TACACNNNNA TGCANNCATG ANNTCTGANN ACCCNANNGG
        961 NGNTCNNNTT ANNAAANCAC TGNNNNCNNN NNNNNNN
        //
        Primer: EGFP-N_R2 (Pr0905)
        Region: insert mid(EGFP 5',AID 3')
        Sequence file: RDB16761_A8K6h.seq check
        >16761_16761_B2K7F_EGFP-N_R2_B01_02_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NANCNNNNCA CGCCNNNNGN CAGGGNGNNN
           61 NNGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC
          121 TTGCCGTAGG TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG
          181 TCGCCGTCCA GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC
          241 ACTTTATACA TCCTCAAATC GATTTTCCTC AAGTACGGTG CTCCGTCCAT TGATACCTTC
          301 ACGAACGCCG CCGCCTCCGG GCCACCGCTT GATTTTTGGC AGGAAACCAT CACGTTCTTC
          361 CGGTATGATC TCACCGGTGG CCATCCCACA ACTTGTGCCT TGGCTGGAGG TTTGGCTGGA
          421 TCTTTAGGAC AAGCACTCTT CTCCTTGGAA TCAAAAGTCA CGACGTCATG AGTCGTAGAT
          481 CCTTCCTTGT TTGCAGGCTC ATTATTCAGA TTTAGCTTCA GATCAACCGT CTCTGAGAAC
          541 CCTCTCTTGT TTCCGGTTAC CGGAGCCACT GTATCTCCAC CGGGAAGANC AAGACACAGC
          601 TCAGTCTCCC TCAGANTCAG CTCGACNCTG CCTCTGACGT ACCAAACATA AACAAACAGT
          661 GAAGCANGNT NNANNGNTCT CACCCNTTCT GGAAAACAGA NGANNACGAC GANAACCTTN
        721 GTNNNTGCNT NNAANNNGNN AANNNNNNTN NNCCNNNNNN NNNN
        //
        Primer: SV40pro_F (Pr0110)
        Region: SV40 pro_ori,NeoR
        Sequence file: RDB16761_A8K6i.seq check
        >D06043A1_A8K6_1_SV40pro_F_D04_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNANTGNNG NNGCTTTTTT GGANGCCTAG GCTTTTGCAA
           61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
          121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
          181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
          241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
          301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
          361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
          421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
          481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
          541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
          601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
          661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
          721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
          781 TCANGACATA GCGTTGGCTA CNCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGNNCTGA
          841 NCGCTTCCTC GNGCTTTACG GTATCGCCGC NCCCGATTCG CANCGCATCG CCTTCTATCG
          901 NCTTCTTGAN GAGTNCTTCN GANCGGGANT CTNGGGNNNC GCNNANNGAN NNNNCNANNN
        961 ANNNCNNANN NNNNCNNNNN NNNNN
        //

        Please visit Sequencing and PCR primers for primer information.

        References

        Original, user report and related articles

        original Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504.

        2023.05.01

        GNP_filter3_RDBDEP_html_230501.pl