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pcDNA-flag-humanPLCz-AID-EGFP-pA (#RDB16761)

Expression vector of human phospholipase C zeta (PLC zeta) tagged with mAID degron.

Clone info. Expression vector of human phospholipase C zeta (PLC zeta) mRNA synthesis, tagged with FLAG at N-terminus and AID-EGFP cassette at C-terminus. Auxin-inducible degron (AID) system clone for in vitro transcription, which has an extended poly (A) sequence.
Comment Expression was confirmed by the depositor with EGFP monitoring by microinjection of its mRNA into mouse oocytes.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human PLCz cDNA Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Ogura, Atsuo |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sato, K. et al. Anim. Sci. J. 84 (4): 359-368, 2013; Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) and an acknowledgment to the DEPOSITOR (Dr. Junya Ito, Azabu University, Dr. Atsuo Ogura, RIKEN BRC) are requested.
Additional terms and conditions:
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Sato, K. et al. Anim. Sci. J. 84 (4): 359-368, 2013; Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018） を引用し、寄託者 (麻布大学　伊藤潤哉、RIKEN BRC 小倉淳郎) への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16761 pcDNA-flag-humanPLCz-AID-EGFP-pA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA-flag-humanPLCz-AID-EGFP-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16761).

Reference section:

Miura, K., Matoba, S., Ogonuki, N., Namiki, T., Ito, J., Kashiwazaki, N., Ogura, A., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16761_A8K6p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,FLAG,insert 5'(hPLCZ1 5')
Sequence file: RDB16761_A8K6e.seq check
>D06043A1_A8K6_1_CMV-Forward_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NTGNNGCAAA NGGGCGGTAN GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGTTAAG CTTATGGACT ACAAAGACGA
  181 TGACGACAAG GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCACG AAATGAGATG
  241 GTTTTTGTCA AAGATTCAGG ATGACTTCAG AGGTGGAAAA ATTAACCTAG AAAAAACTCA
  301 GAGGTTACTT GAAAAATTAG ATATTCGGTG CAGTTATATT CATGTGAAAC AGATTTTTAA
  361 GGACAATGAC AGGCTGAAAC AAGGAAGAAT CACCATAGAA GAATTTAGAG CAATTTATCG
  421 AATTATCACG CACAGAGAAG AAATTATTGA GATTTTCAAC ACATATTCTG AAAACCGGAA
  481 AATTCTTTTA GCAAGTAATC TGGCTCAATT TCTGACACAA GAACAATATG CAGCTGAGAT
  541 GAGTAAAGCT ATTGCTTTTG AGATCATTCA GAAATACGAG CCTATCGAAG AAGTTAGGAA
  601 AGCACACCAA ATGTCATTAG AAGGTTTTAC AAGATACATG GATTCACGTG AATGTCTACT
  661 GTTTAAAAAT GAATGTAGAA AAGTTTATCA AGATATGACT CATCCATTAA ATGATTATTT
  721 TATTTCATCT TCACATAACA CATATTTGGT ATCTGATCAA TTATTGNGAC CAAGTGANCT
  781 TTGGGGATAT GTAAGTGCCC TTGTGAAAGG ATGCCGTTGT TTGGAGATTG ACTGCTGGGA
  841 TGGAGCACAA AATGAACCTG TTGTATATCA TGGCTACNCA CTCACAAGCA AACTTCTGTT
  901 NNAAACTGNT ATCCNAGCTA TACACNNNNA TGCANNCATG ANNTCTGANN ACCCNANNGG
961 NGNTCNNNTT ANNAAANCAC TGNNNNCNNN NNNNNNN
//
Primer: GNP Primer #10 (Pr0763)
Region: TRF2-3'UTR + polyA83,insert 3'(EGFP 3')
Sequence file: RDB16761_A8K6f.seq check
>D06043A1_A8K6_1_GNP_Primer#10_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNTNATTTN NNNGNNAACG TTTACNGGTT CTTTGGTGCC CATAGTCGAG
   61 CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCTTATTAC TTGTACAGCT CGTCCATGCC
  121 GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT
  181 GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG
  241 GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC
  301 GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT
  361 ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC
  421 CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC
  481 CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA
  541 GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT
  601 GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT
  661 GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAN GTGGCATCGC CCTCGCCCTC
  721 GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC
  781 CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACTTTATAC NTCCTCAAAT CGANTTTCCT
  841 CAAGTACGGN GCTCCGTCCN TTGATACCTT CACGAACGCC GCCGCCTCCN GGGCCACNGC
  901 TNGNTTTTTN NNANGAAACC NTCNNGTNCT TCCNGNATGA NCNCNNNNNN GGNNATCCNN
961 NNNCNNNNNN NNNN
//
Primer: EGFP-N (Pr0027)
Region: insert mid(AID 5',hPLCZ1 3')
Sequence file: RDB16761_A8K6g.seq check
>D06043A1_A8K6_1_CMV-Forward_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NTGNNGCAAA NGGGCGGTAN GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGTTAAG CTTATGGACT ACAAAGACGA
  181 TGACGACAAG GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCACG AAATGAGATG
  241 GTTTTTGTCA AAGATTCAGG ATGACTTCAG AGGTGGAAAA ATTAACCTAG AAAAAACTCA
  301 GAGGTTACTT GAAAAATTAG ATATTCGGTG CAGTTATATT CATGTGAAAC AGATTTTTAA
  361 GGACAATGAC AGGCTGAAAC AAGGAAGAAT CACCATAGAA GAATTTAGAG CAATTTATCG
  421 AATTATCACG CACAGAGAAG AAATTATTGA GATTTTCAAC ACATATTCTG AAAACCGGAA
  481 AATTCTTTTA GCAAGTAATC TGGCTCAATT TCTGACACAA GAACAATATG CAGCTGAGAT
  541 GAGTAAAGCT ATTGCTTTTG AGATCATTCA GAAATACGAG CCTATCGAAG AAGTTAGGAA
  601 AGCACACCAA ATGTCATTAG AAGGTTTTAC AAGATACATG GATTCACGTG AATGTCTACT
  661 GTTTAAAAAT GAATGTAGAA AAGTTTATCA AGATATGACT CATCCATTAA ATGATTATTT
  721 TATTTCATCT TCACATAACA CATATTTGGT ATCTGATCAA TTATTGNGAC CAAGTGANCT
  781 TTGGGGATAT GTAAGTGCCC TTGTGAAAGG ATGCCGTTGT TTGGAGATTG ACTGCTGGGA
  841 TGGAGCACAA AATGAACCTG TTGTATATCA TGGCTACNCA CTCACAAGCA AACTTCTGTT
  901 NNAAACTGNT ATCCNAGCTA TACACNNNNA TGCANNCATG ANNTCTGANN ACCCNANNGG
961 NGNTCNNNTT ANNAAANCAC TGNNNNCNNN NNNNNNN
//
Primer: EGFP-N_R2 (Pr0905)
Region: insert mid(EGFP 5',AID 3')
Sequence file: RDB16761_A8K6h.seq check
>16761_16761_B2K7F_EGFP-N_R2_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NANCNNNNCA CGCCNNNNGN CAGGGNGNNN
   61 NNGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC
  121 TTGCCGTAGG TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG
  181 TCGCCGTCCA GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC
  241 ACTTTATACA TCCTCAAATC GATTTTCCTC AAGTACGGTG CTCCGTCCAT TGATACCTTC
  301 ACGAACGCCG CCGCCTCCGG GCCACCGCTT GATTTTTGGC AGGAAACCAT CACGTTCTTC
  361 CGGTATGATC TCACCGGTGG CCATCCCACA ACTTGTGCCT TGGCTGGAGG TTTGGCTGGA
  421 TCTTTAGGAC AAGCACTCTT CTCCTTGGAA TCAAAAGTCA CGACGTCATG AGTCGTAGAT
  481 CCTTCCTTGT TTGCAGGCTC ATTATTCAGA TTTAGCTTCA GATCAACCGT CTCTGAGAAC
  541 CCTCTCTTGT TTCCGGTTAC CGGAGCCACT GTATCTCCAC CGGGAAGANC AAGACACAGC
  601 TCAGTCTCCC TCAGANTCAG CTCGACNCTG CCTCTGACGT ACCAAACATA AACAAACAGT
  661 GAAGCANGNT NNANNGNTCT CACCCNTTCT GGAAAACAGA NGANNACGAC GANAACCTTN
721 GTNNNTGCNT NNAANNNGNN AANNNNNNTN NNCCNNNNNN NNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR
Sequence file: RDB16761_A8K6i.seq check
>D06043A1_A8K6_1_SV40pro_F_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNANTGNNG NNGCTTTTTT GGANGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  781 TCANGACATA GCGTTGGCTA CNCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGNNCTGA
  841 NCGCTTCCTC GNGCTTTACG GTATCGCCGC NCCCGATTCG CANCGCATCG CCTTCTATCG
  901 NCTTCTTGAN GAGTNCTTCN GANCGGGANT CTNGGGNNNC GCNNANNGAN NNNNCNANNN
961 ANNNCNNANN NNNNCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504. [link to RRC of NBRP]