RDB16761 - pcDNA-flag-humanPLCz-AID-EGFP-pA

Resource data sheet
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pcDNA-flag-humanPLCz-AID-EGFP-pA

Expression vector of human phospholipase C zeta (PLC zeta) tagged with mAID degron.

Catalog number	RDB16761
Resource name	pcDNA-flag-humanPLCz-AID-EGFP-pA
Clone info.	Expression vector of human phospholipase C zeta (PLC zeta) mRNA synthesis, tagged with FLAG at N-terminus and AID-EGFP cassette at C-terminus. Auxin-inducible degron (AID) system clone for in vitro transcription, which has an extended poly (A) sequence. Expression was confirmed by the depositor with EGFP monitoring by microinjection of its mRNA into mouse oocytes.
Vector backbone	pcDNA3.1-pA83 (Plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	Human PLCz cDNA Arabidopsis thaliana AtIAA17 cDNA
Depositor	Ogura, Atsuo \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sato, K. et al. Anim. Sci. J. 84 (4): 359-368, 2013; Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) and an acknowledgment to the DEPOSITOR (Dr. Junya Ito, Azabu University, Dr. Atsuo Ogura, RIKEN BRC) are requested.

Additional terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sato, K. et al. Anim. Sci. J. 84 (4): 359-368, 2013; Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) and an acknowledgment to the DEPOSITOR (Dr. Junya Ito, Azabu University, Dr. Atsuo Ogura, RIKEN BRC) are requested.
Additional terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献（Sato, K. et al. Anim. Sci. J. 84 (4): 359-368, 2013; Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018）を引用し、寄託者 (麻布大学　伊藤潤哉、RIKEN BRC 小倉淳郎) への謝辞の表明を必要とする。
付加的提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16761	pcDNA-flag-humanPLCz-AID-EGFP-pA	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB16761_A8K6p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward	RDB16761_A8K6a.seq
>D06043A1_A8K6_1_CMV-Forward_A04_01_ABI24.ab1 1 CTAATCAAAC TCCGCCCCAT TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT 61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA 121 ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGTTAAG CTTATGGACT ACAAAGACGA 181 TGACGACAAG GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCACG AAATGAGATG 241 GTTTTTGTCA AAGATTCAGG ATGACTTCAG AGGTGGAAAA ATTAACCTAG AAAAAACTCA 301 GAGGTTACTT GAAAAATTAG ATATTCGGTG CAGTTATATT CATGTGAAAC AGATTTTTAA 361 GGACAATGAC AGGCTGAAAC AAGGAAGAAT CACCATAGAA GAATTTAGAG CAATTTATCG 421 AATTATCACG CACAGAGAAG AAATTATTGA GATTTTCAAC ACATATTCTG AAAACCGGAA 481 AATTCTTTTA GCAAGTAATC TGGCTCAATT TCTGACACAA GAACAATATG CAGCTGAGAT 541 GAGTAAAGCT ATTGCTTTTG AGATCATTCA GAAATACGAG CCTATCGAAG AAGTTAGGAA 601 AGCACACCAA ATGTCATTAG AAGGTTTTAC AAGATACATG GATTCACGTG AATGTCTACT 661 GTTTAAAAAT GAATGTAGAA AAGTTTATCA AGATATGACT CATCCATTAA ATGATTATTT 721 TATTTCATCT TCACATAACA CATATTTGGT ATCTGATCAA TTATTGGGAC CAAGTGACCT 781 TTGGGGATAT GTAAGTGCCC TTGTGAAAGG ATGCCGTTGT TTGGAGATTG ACTGCTGGGA 841 TGGAGCACAA AATGAACCTG TTGTATATCA TGGCTACACA CTCACAAGCA AACTTCTGTT 901 TAAAACTGTT ATCCAAGCTA TACACAAGTA TGCATTCATG ACATCTGACT ACCCCAGTGG 961 TGCTCTCTTT AGAAAATCAC TGCCTCCCAC CTGCCCCAAC CAAGAAAGTA ATGGTCAGAC 1021 AATTTTGCAG CTACTTTTTG GAGAGTCCTT GCTTTTCTGG ATATGGCTTG ATGATTTTTC 1081 TTGATTACCT CCTAACCCAA TTCACCTAGA GTCCAACTTT AAAAATTCCT AAAAGAT //
Primer: EGFP-N	RDB16761_A8K6b.seq
>D06043A1_A8K6_1_EGFP-N_B04_04_ABI24.ab1 1 GGACCGGGGT GACGCTCCTC GCCCTTGCTC ACTTTATACA TCCTCAAATC GATTTTCCTC 61 AAGTACGGTG CTCCGTCCAT TGATACCTTC ACGAACGCCG CCGCCTCCGG GCCACCGCTT 121 GATTTTTGGC AGGAAACCAT CACGTTCTTC CGGTATGATC TCACCGGTGG CCATCCCACA 181 ACTTGTGCCT TGGCTGGAGG TTTGGCTGGA TCTTTAGGAC AAGCACTCTT CTCCTTGGAA 241 TCAAAAGTCA CGACGTCATG AGTCGTAGAT CCTTCCTTGT TTGCAGGCTC ATTATTCAGA 301 TTTAGCTTCA GATCAACCGT CTCTGAGAAC CCTCTCTTGT TTCCGGTTAC CGGAGCCACT 361 GTATCTCCAC CGGGAAGACC AAGACACAGC TCAGTCTCCC TCAGATTCAG CTCGACACTG 421 CCTCTGACGT ACCAAACATA AACAAACAGT GAAGCAGGCT CAAGGCTCTC ACCCATTCTG 481 GAAAACAGAG GAATACGACG ATAACCTTTG TTCATGCATA GAAGTGGCAA AGTATATTGC 541 CCAAGAAATT CATTTCCTGC TATTAAACCT TGACCTTCAA CAACAAAACG TATCAATGCC 601 AATTCTGGGA CATGAATAAT AAATGTGAAT GTTTCATTCC ATCTTGGACT AAAAGCATTT 661 TTTTTAATTA CACGAGTCTG CTGCTTCATT TGATCATTTG GAACACCAAA AACTTCTATA 721 ATTACTAATG AATCACCTTT GTTAGATGAT GAATGAGTAA GAGGCAACTG GATACCACTG 781 ATGAGCCTTA TTGTAAGTGT AATTGGCATA CCCTCTTTTA TGTTACTTGG GTTAAAGTAT 841 GATTTACTCT CTCTTAAGAA ATGTGGTTTC AAAATATATC CAGAACCACC ATTATCCAAA 901 AAATTTCCCA TTTTGCAGAT CCATGGGGCA GACCAGGGGT CTGGGAAATT TAAAGCCACC 961 ATTTGACAAC CTATATTCCC AAAATTCTTT GGGGGATTTA AAAATTTAGA AGAGTCTGCT 1021 CTTTGGTTGC TTTGGGATAT ATTCTGGTAA TTGACTTTCC TTGCTGTGAA AAATAAACTC 1081 ATGGACTCGC ATTTTTGAAG TTTTTCGGGC TTTGGGGTGT C //
Primer: GNP_primer#10	RDB16761_A8K6c.seq
>D06043A1_A8K6_1_GNP_Primer#10_C04_07_ABI24.ab1 1 TTATTTGTGC CATTCATTTA AAAGATAACG TTTACAGGTT CTTTGGTGCC CATAGTCGAG 61 CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCTTATTAC TTGTACAGCT CGTCCATGCC 121 GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT 181 GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG 241 GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC 301 GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT 361 ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC 421 CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC 481 CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA 541 GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT 601 GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT 661 GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC 721 GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC 781 CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACTTTATAC ATCCTCAAAT CGATTTTCCT 841 CAAGTACGGT GCTCCGTCCA TTGATACCTT CACGAACGCC GCCGCCTCCG GGGCCACCGC 901 TTGATTTTTG GCAGGAAACC ATCACGTTCT TCCGGTATGA TCTCACCGGT GGCCATCCCA 961 CAACTTGTGC CTTGGCTGGA GGTTTGGCTG GATCTTTAGG ACAGCACTCT TTCTCCTTGG 1021 GATTCATAGT CACGAACGTC ATGAGTCGTA GATCGACCTG ATTGCCAGGT CTCATATCCA 1081 GATTAGCCTT TCCAGAATCA CCGATTCTTC TGGAAGAAA //
Primer: SV40pro_F	RDB16761_A8K6d.seq
>D06043A1_A8K6_1_SV40pro_F_D04_10_ABI24.ab1 1 CATTGTTCCT GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC 121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GCGAAATGAC CGACCAAGCG 961 ACGCCCAACC CTGCCCATCA CGAGATTTCG ATTCCACCCG CCCGCCTTTC TATGAAAGGT 1021 TGGGCTTTCC GGATCGTTTT CCGGGACGCC GGCTTGGAAT GATTCTCAGC GCGGGGATCT 1081 CATGCTGGAA GTCTTCGGCT CAACCCTCCG ACTGGTTTTA TTTGGCCAAG G //

Please visit Sequencing and PCR primers for primer information.

References

original	Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504.

2018.12.14

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