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pcDNA-flag-humanPLCz-AID-EGFP-pA

Expression vector of human phospholipase C zeta (PLC zeta) tagged with mAID degron.

Catalog number RDB16761
Resource name pcDNA-flag-humanPLCz-AID-EGFP-pA
Clone info. Expression vector of human phospholipase C zeta (PLC zeta) mRNA synthesis, tagged with FLAG at N-terminus and AID-EGFP cassette at C-terminus. Auxin-inducible degron (AID) system clone for in vitro transcription, which has an extended poly (A) sequence.
Expression was confirmed by the depositor with EGFP monitoring by microinjection of its mRNA into mouse oocytes.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human PLCz cDNA Arabidopsis thaliana AtIAA17 cDNA
Depositor Ogura, Atsuo |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sato, K. et al. Anim. Sci. J. 84 (4): 359-368, 2013; Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) and an acknowledgment to the DEPOSITOR (Dr. Junya Ito, Azabu University, Dr. Atsuo Ogura, RIKEN BRC) are requested.
Additional terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB16761 pcDNA-flag-humanPLCz-AID-EGFP-pA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16761_A8K6p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB16761_A8K6a.seq
>D06043A1_A8K6_1_CMV-Forward_A04_01_ABI24.ab1
    1 CTAATCAAAC TCCGCCCCAT TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGTTAAG CTTATGGACT ACAAAGACGA
  181 TGACGACAAG GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCACG AAATGAGATG
  241 GTTTTTGTCA AAGATTCAGG ATGACTTCAG AGGTGGAAAA ATTAACCTAG AAAAAACTCA
  301 GAGGTTACTT GAAAAATTAG ATATTCGGTG CAGTTATATT CATGTGAAAC AGATTTTTAA
  361 GGACAATGAC AGGCTGAAAC AAGGAAGAAT CACCATAGAA GAATTTAGAG CAATTTATCG
  421 AATTATCACG CACAGAGAAG AAATTATTGA GATTTTCAAC ACATATTCTG AAAACCGGAA
  481 AATTCTTTTA GCAAGTAATC TGGCTCAATT TCTGACACAA GAACAATATG CAGCTGAGAT
  541 GAGTAAAGCT ATTGCTTTTG AGATCATTCA GAAATACGAG CCTATCGAAG AAGTTAGGAA
  601 AGCACACCAA ATGTCATTAG AAGGTTTTAC AAGATACATG GATTCACGTG AATGTCTACT
  661 GTTTAAAAAT GAATGTAGAA AAGTTTATCA AGATATGACT CATCCATTAA ATGATTATTT
  721 TATTTCATCT TCACATAACA CATATTTGGT ATCTGATCAA TTATTGGGAC CAAGTGACCT
  781 TTGGGGATAT GTAAGTGCCC TTGTGAAAGG ATGCCGTTGT TTGGAGATTG ACTGCTGGGA
  841 TGGAGCACAA AATGAACCTG TTGTATATCA TGGCTACACA CTCACAAGCA AACTTCTGTT
  901 TAAAACTGTT ATCCAAGCTA TACACAAGTA TGCATTCATG ACATCTGACT ACCCCAGTGG
  961 TGCTCTCTTT AGAAAATCAC TGCCTCCCAC CTGCCCCAAC CAAGAAAGTA ATGGTCAGAC
 1021 AATTTTGCAG CTACTTTTTG GAGAGTCCTT GCTTTTCTGG ATATGGCTTG ATGATTTTTC
1081 TTGATTACCT CCTAACCCAA TTCACCTAGA GTCCAACTTT AAAAATTCCT AAAAGAT
//
Primer: EGFP-N RDB16761_A8K6b.seq
>D06043A1_A8K6_1_EGFP-N_B04_04_ABI24.ab1
    1 GGACCGGGGT GACGCTCCTC GCCCTTGCTC ACTTTATACA TCCTCAAATC GATTTTCCTC
   61 AAGTACGGTG CTCCGTCCAT TGATACCTTC ACGAACGCCG CCGCCTCCGG GCCACCGCTT
  121 GATTTTTGGC AGGAAACCAT CACGTTCTTC CGGTATGATC TCACCGGTGG CCATCCCACA
  181 ACTTGTGCCT TGGCTGGAGG TTTGGCTGGA TCTTTAGGAC AAGCACTCTT CTCCTTGGAA
  241 TCAAAAGTCA CGACGTCATG AGTCGTAGAT CCTTCCTTGT TTGCAGGCTC ATTATTCAGA
  301 TTTAGCTTCA GATCAACCGT CTCTGAGAAC CCTCTCTTGT TTCCGGTTAC CGGAGCCACT
  361 GTATCTCCAC CGGGAAGACC AAGACACAGC TCAGTCTCCC TCAGATTCAG CTCGACACTG
  421 CCTCTGACGT ACCAAACATA AACAAACAGT GAAGCAGGCT CAAGGCTCTC ACCCATTCTG
  481 GAAAACAGAG GAATACGACG ATAACCTTTG TTCATGCATA GAAGTGGCAA AGTATATTGC
  541 CCAAGAAATT CATTTCCTGC TATTAAACCT TGACCTTCAA CAACAAAACG TATCAATGCC
  601 AATTCTGGGA CATGAATAAT AAATGTGAAT GTTTCATTCC ATCTTGGACT AAAAGCATTT
  661 TTTTTAATTA CACGAGTCTG CTGCTTCATT TGATCATTTG GAACACCAAA AACTTCTATA
  721 ATTACTAATG AATCACCTTT GTTAGATGAT GAATGAGTAA GAGGCAACTG GATACCACTG
  781 ATGAGCCTTA TTGTAAGTGT AATTGGCATA CCCTCTTTTA TGTTACTTGG GTTAAAGTAT
  841 GATTTACTCT CTCTTAAGAA ATGTGGTTTC AAAATATATC CAGAACCACC ATTATCCAAA
  901 AAATTTCCCA TTTTGCAGAT CCATGGGGCA GACCAGGGGT CTGGGAAATT TAAAGCCACC
  961 ATTTGACAAC CTATATTCCC AAAATTCTTT GGGGGATTTA AAAATTTAGA AGAGTCTGCT
 1021 CTTTGGTTGC TTTGGGATAT ATTCTGGTAA TTGACTTTCC TTGCTGTGAA AAATAAACTC
1081 ATGGACTCGC ATTTTTGAAG TTTTTCGGGC TTTGGGGTGT C
//
Primer: GNP_primer#10 RDB16761_A8K6c.seq
>D06043A1_A8K6_1_GNP_Primer#10_C04_07_ABI24.ab1
    1 TTATTTGTGC CATTCATTTA AAAGATAACG TTTACAGGTT CTTTGGTGCC CATAGTCGAG
   61 CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCTTATTAC TTGTACAGCT CGTCCATGCC
  121 GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT
  181 GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG
  241 GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC
  301 GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT
  361 ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC
  421 CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC
  481 CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA
  541 GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT
  601 GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT
  661 GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC
  721 GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC
  781 CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACTTTATAC ATCCTCAAAT CGATTTTCCT
  841 CAAGTACGGT GCTCCGTCCA TTGATACCTT CACGAACGCC GCCGCCTCCG GGGCCACCGC
  901 TTGATTTTTG GCAGGAAACC ATCACGTTCT TCCGGTATGA TCTCACCGGT GGCCATCCCA
  961 CAACTTGTGC CTTGGCTGGA GGTTTGGCTG GATCTTTAGG ACAGCACTCT TTCTCCTTGG
 1021 GATTCATAGT CACGAACGTC ATGAGTCGTA GATCGACCTG ATTGCCAGGT CTCATATCCA
1081 GATTAGCCTT TCCAGAATCA CCGATTCTTC TGGAAGAAA
//
Primer: SV40pro_F RDB16761_A8K6d.seq
>D06043A1_A8K6_1_SV40pro_F_D04_10_ABI24.ab1
    1 CATTGTTCCT GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GCGAAATGAC CGACCAAGCG
  961 ACGCCCAACC CTGCCCATCA CGAGATTTCG ATTCCACCCG CCCGCCTTTC TATGAAAGGT
 1021 TGGGCTTTCC GGATCGTTTT CCGGGACGCC GGCTTGGAAT GATTCTCAGC GCGGGGATCT
1081 CATGCTGGAA GTCTTCGGCT CAACCCTCCG ACTGGTTTTA TTTGGCCAAG G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504.

2018.12.14

GNP_filter3_RDBDEP_html_181012.pl