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Yeast NADPH-P450 reductase/pBKS-

Cloning vector of S. cerevisiae NADPH-P450 reductase.

Catalog number RDB16760
Resource name Yeast NADPH-P450 reductase/pBKS-
Clone info. Cloning vector of S. cerevisiae NADPH-P450 reductase.
Known differences (at least): substitutions G to A (Pro, synonym) at 641nt, A to C (Asn to Thr) at 1903nt and G to T (Gly to Val) at 2056nt of CP036477.1.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Saccharomyces cerevisiae NADPH-P450 reductase cDNA
Depositor Aoyama, Yuri |
 
Other clones in our bank Saccharomyces cerevisiae NCP1 (KEGG orthology K00327) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kitahama, Y et al. Biol. Pharm. Bull. 32(4), 558-63, 2009) is requested.
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RDB16760 Yeast NADPH-P450 reductase/pBKS- DNA solution

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References and tips

Electronic file

Original reference

original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16760_A8K1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: insert 5' | Primer: M13_-40 | Sequence: RDB16760_A8K1a.seq
>D05837A8_A8K1_2_M13_-40_D03_12_ABI24.ab1
    1 AAGCCAATTG GCGCGCGGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGCGGT
   61 GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCGATCA GGATCCATCG
  121 ATGCTTAGGA GGTCATATGC CATTTGGAAT AGACAACACC GACTTCACTG TCCTGGCGGG
  181 GCTAGTGCTT GCCGTGCTAC TGTACGTAAA GAGAAACTCC ATCAAGGAAC TGCTGATGTC
  241 CGATGACGGA GATATCACAG CTGTCAGCTC GGGCAACAGA GACATTGCTC AGGTGGTGAC
  301 CGAAAACAAC AAGAACTACT TGGTGTTGTA TGCGTCGCAG ACTGGGACTG CCGAGGATTA
  361 CGCCAAAAAG TTTTCCAAGG AGCTGGTGGC CAAGTTCAAC CTAAACGTGA TGTGCGCAGA
  421 TGTTGAGAAC TACGACTTTG AGTCGCTAAA CGATGTGCCC GTCATAGTCT CGATTTTTAT
  481 CTCTACATAT GGTGAAGGAG ACTTCCCCGA CGGGGCGGTC AACTTTGAAG ACTTTATTTG
  541 TAATGCGGAA GCGGGTGCAC TATCGAACCT GAGGTATAAT ATGTTTGGTC TGGGAAATTC
  601 TACTTATGAA TTCTTTAATG GTGCCGCCAA GAAGGCCGAG AAGCATCTCT CCGCCGCGGG
  661 CGCTATCAGA CTAGGCAAGC TCGGTGAAGC TGATGATGGT GCAGGAACTA CAGACGAAGA
  721 TTACATGGCC TGGAAGGACT CCATCCTGGA GGTTTTGAAA GACGAACTGC ATTTGGACGA
  781 ACAGGAAGCC AAGTTCACCT CTCAATTCCA GTACACTGTG TTGAACGAAA TCACTGACTC
  841 CATGTCGCTT GGTGAACCCT CTGCTCACTA TTTGCCCTCG CATCAGTTGA ACCGCAACGC
  901 AGACGGCATC CAATTGGGTC CCTTCGATTT GTCTCAACCG TATATTGCAC CCATCGTGAA
  961 ATCTCGCGAA CTGTTCTCTT CCAATGACCG TAATTGCATC CACTCTGAAT TTGACTTGTC
 1021 CGGCTCTAAC ATCAAGTACT CCACTGGTGA CCATCTTGCT GTTGGCCTTC CAACCCATTT
 1081 GGAAAAGGTC GACAGTTCTT ATCCATATTT CACCCTGGCA CCTGGAATCA TTTTTGACTT
1141 GGAAGCCCTG ATCCCAACGT TCCAATGCCT TCCGCGCTGA ACCTACCTAT TTAGC
//
Region: insert 3' | Primer: Reverse2 | Sequence: RDB16760_A8K1b.seq
>D05837A8_A8K1_2_reverse2_E03_15_ABI24.ab1
    1 AGGTGACAAT GATTAACGCA AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
   61 GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGAGGAAGCT TTTACCAGAC
  121 ATCTTCTTGG TATCTACCTG AAGTCTTGAG CATCTTGATT AGCTCTGTTG CTTCATCAGT
  181 GGTAATGGAT TTACCACGGG ATAAGATGCC AACCAATGCG GTTGACACAC CCTTGGCCAT
  241 ACCCTTTGCA TCACCACAGA CGTAGATAAA TGCACCGTTG TTAATCATTT CAAATACTTG
  301 GTCTTCGTAA TCCTTTAATT TATCTTGAAC ATAAACTTTT TTGGTGTTTG GCAACCTGGA
  361 ATGGGCCACG ACCATTTCGA ACGAACCATC CAATTTTTTG GCGTATTCTG GCCATTCGTC
  421 CTGGTACAAG AAATCATCAG TGTTACGGGA TCCATAAAAC AGTATATGCT TACCTAGCGA
  481 AACGTTGTTA CCGCCCTTCT TTTGTGATTC GAGGAACGCG ACACGCTCTC TGATAAACCC
  541 ACGGAATGGG GCAACACCGG TACCTGGACC GATCATGATA ACTGGGGTGG AAGGGTTGGA
  601 AGGCAATCTG AAGTTAGAAC GACGAACGTG GACGGGCAAT TTGTAATTGG CGAAAAGTTT
  661 ACGTGGGCCA TTTAAATCGT AGTGAACAGG TAGGTTAGTT TCGGCAATGT TAACATTGTT
  721 TTGAGCCAAT TGAATGTTTC TTAACAAGTT AGTCGTAACA CCAACAACTG GAGGAGCATC
  781 AGGCAATTCT GGGTTAGGAA AGTTTTCCAC AATGGAGGTG ACATGGACGG TTTGCTTTTC
  841 AGACAGAGAA GAGGAAGAGA TAGAGTAGTA ACGAGGAGTC ATTTGGGGAA CTGATTCGAC
  901 CAAGAATTGC ATGGGTACGG TGTCCCATTT GGCGCCATCA GACAAATATT TCAGAGCATC
  961 TGCGATGTTG AAATATTTGG AGGTTATCTC GACGGCGAAT TGGTCCTTGT CTTTCGAAAG
 1021 CAGAGTCAAT TTTTTCCTTG ACGTCAGCGT TGGGGGCGAA CTGAATCAAA AGATGAAAAC
 1081 CATTGTCTGG GAGACAGGTC CTGTATTTTC AAATAGTGTT TAATAGCAGC GCCAATAGTA
 1141 GTGCCGTGGA GGGCACTTTG ACGGTGGGAT CCAGGGGCTT CAAGGTCAAA AAAATTGGGT
1201 C
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884.

2019.08.23

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