Sequencing and PCR primers for primer information.
Cloning vector of G310D variant of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.
| Catalog number | RDB16757 |
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| Resource name | Yeast mutant CYP51F1m/pBKS- |
| Clone info. | Cloning vector of G310D variant of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region. Modified to ATGGCTCTCT TATTAGCAGT TTTT from ATGTCTGCTA CCAAGTCAAT CGTT of nucleic acid sequence coding N-terminal 10 amino acid residues. |
| Vector backbone | pBluescript KS- (plasmid) |
| Size of vector backbone | 3.0 kb |
| Selectable markers | Amp^r |
| Gene/insert name | Saccharomyces cerevisiae mutated CYP51F1(erg11) cDNA |
| Depositor | Aoyama, Yuri | |
| Other clones in our bank | Saccharomyces cerevisiae ERG11 (KEGG orthology K05917) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ishida, N et al. Biochem. Biophys. Res. Commun. 155(1), 317-23, 1988) is requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16757 | Yeast mutant CYP51F1m/pBKS- | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
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RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16757_A8K1p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Region: insert 5' | Primer: M13_-40 | Sequence: RDB16757_A8K1a.seq |
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>D05837A5_A8K1_3_M13_-40_F02_17_ABI24.ab1 1 AAGTCAGCGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CACCGCGGTG 61 GCGGCCGCTC TAGAACTAGT GGATCCATCG ATGCTTAGGA GGTCATATGG CTCTGTTATT 121 AGCAGTTTTT GGAGAGGCAT TGGAATACGT AAACATTGGT TTAAGTCATT TCTTGGCTTT 181 ACCATTGGCC CAAAGAATCT CTTTGATCAT AATAATTCCT TTCATTTACA ATATTGTATG 241 GCAATTACTA TATTCTTTGA GAAAGGACCG TCCACCTCTA GTGTTTTACT GGATTCCATG 301 GGTCGGTAGT GCTGTTGTGT ACGGTATGAA GCCATACGAG TTTTTCGAAG AATGTCAAAA 361 GAAATACGGT GATATTTTTT CATTCGTTTT GTTAGGAAGA GTCATGACTG TGTATTTAGG 421 ACCAAAGGGT CACGAATTTG TCTTCAACGC TAAGTTGGCA GATGTTTCAG CAGAAGCTGC 481 TTACGCTCAT TTGACTACTC CAGTTTTCGG TAAAGGTGTT ATTTACGATT GTCCAAATTC 541 TAGATTGATG GAGCAAAAGA AGTTTGTTAA GGGTGCTCTA ACCAAAGAAG CCTTCAAGAG 601 CTACGTTCCA TTGATTGCTG AAGAAGTGTA CAAGTACTTC AGAGACTCCA AAAACTTCCG 661 TTTGAATGAA AGAACTACTG GTACTATTGA CGTGATGGTT ACTCAACCTG AAATGACTAT 721 TTTCACCGCT TCAAGATCAT TATTGGGTAA GGAAATGAGA GCAAAATTGG ATACCGATTT 781 TGCTTACTTG TACAGTGATT TGGATAAGGG TTTCACTCCA ATCAACTTCG TCTTCCCTAA 841 CTTACCATTG GAACACTATA GAAAGAGAGA TCACGCTCAA AAGGCTATCT CCGGTACTTA 901 CATGTCTTTG ATTAAGGAAA GAAGAAAAGA ACAACGACAT TCAAGACAGA GATTTGATCG 961 ATTCCTTGAT GAAGAACTCT ACCTACAAGG ATGGTGTGAA GATGACTGAT CAAGAATCGC 1021 TAACTTGTTA ATTGATGTCT TAATGGGTGG TCAACATACT TCTGCTGCCA CTTCTGCTTG 1081 GATTTTGTTG CACTTGCTGA AAGACCAGAT GTTCCAACAA GGATGTACGA GAACAATGCT 1141 GTTTTGGATG TTGTAGAAGG ATGACTACGA TTATACAGGA AATGGTGAC // |
| Region: insert 3' | Primer: Reverse2 | Sequence: RDB16757_A8K1b.seq |
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>D05837A5_A8K1_3_reverse2_G02_20_ABI24.ab1 1 AAGGACAATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGGT 61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTT TAGATCTTTT GTTCTGGATT 121 TCTCTTTTCC CAGATGATCT TGGCTGGACC GGTTGGAAGA GTAACCATAG ATGTAAAGTC 181 AGGAGGTGGA ACGGTCTTAC CCTCTGGGTA ATGCCATTTT AATGTTCTGA TAAAAATGGA 241 CATTAGAACA CCTAGCTGAC AGTAAGCAAA GTGTTCACCG ATACATCTGT GTCTACCACC 301 ACCGAAAGGT AAGTATGGAG AGCTGACACC CTTAGAAATG GCACCGAAAC CGTAATCGAC 361 TTCTTCACCG ACGGAATAAG AGGAGGCAGA ATCTTTGTTC CAACGGTGAA TGTTGAATTG 421 GTGAGCATTA GGGAAGTATT CGTCTCTTAA ATGAGTGTAA CCTGGAGAAA CCAAAACGTG 481 ATAACCTGCT GGGATGACAT AAGAAGTGTT TGGAACGTGC ATATCTTTCA TAACCTTACG 541 GAACAAAGAG TGCAATGGAT GGTGCATTCT TAGAGTTTCC TTAATAGTTT GGTTCAACAA 601 TGGCATTTCT TGTAATAAAT CGTAGGTCAA TTCCTTCTTA CCACCATCCA AAACACGCAT 661 TTGTTCTTCG TACAATTCTT GTTGGACATC TGGTCTTTCA GCCAAGTGCA ACAAAATCCA 721 AGCAGAAGTG GCAGCAGAAG TATGTTGACC ACCCATTAAG ACATCAATTA ACAAGTTAGC 781 GATTTCTTGA TCAGTCATCT TCACACCATC CTTGTAGGTA GAGTTCTTCA TCAAGGAATC 841 GATCAAATCT CTGTCTTGAA TGTCGTTGTT CTTTCTTCTT TCCTTAATCA AAGACATGTA 901 AGTACCGGAG ATAGCCTTTT GAGCGTGATC TCTCTTTCTA TAGTGTTCCA ATGGTAAGTT 961 AGGGAAGACG AAGTTGATTG GAGTGAAACC CTTATCCAAA TCACTGTACA AGTAAGCAAA 1021 ATCGGTATCC AATTTTGCTC TCATTTCCTT TACCCAATAA TGATCTGAGC GGTGAAAATA 1081 GTCATTTCAG CTGAGTACAT CACGTCATAG TTACCAGTAG TCTTTCATCC AACAAAGTTT 1141 TGAGTCTCTT GAAGTACTGA ACCGTCTCAG CATTCATGTG TGAC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.08.23
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