Resource data sheet
Prev.

Yeast mutant CYP51F1m/pBKS-

Cloning vector of G310D variant of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.

Catalog number RDB16757
Resource name Yeast mutant CYP51F1m/pBKS-
Clone info. Cloning vector of G310D variant of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.
Modified to ATGGCTCTCT TATTAGCAGT TTTT from ATGTCTGCTA CCAAGTCAAT CGTT of nucleic acid sequence coding N-terminal 10 amino acid residues.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Saccharomyces cerevisiae mutated CYP51F1(erg11) cDNA
Depositor Aoyama, Yuri |
 
Other clones in our bank Saccharomyces cerevisiae ERG11 (KEGG orthology K05917) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ishida, N et al. Biochem. Biophys. Res. Commun. 155(1), 317-23, 1988) is requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16757 Yeast mutant CYP51F1m/pBKS- DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ishida, N., A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS. Biochem. Biophys. Res. Commun. 155 (1): 317-323 (1988). PMID 3046615.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16757_A8K1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: insert 5' | Primer: M13_-40 | Sequence: RDB16757_A8K1a.seq
>D05837A5_A8K1_3_M13_-40_F02_17_ABI24.ab1
    1 AAGTCAGCGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CACCGCGGTG
   61 GCGGCCGCTC TAGAACTAGT GGATCCATCG ATGCTTAGGA GGTCATATGG CTCTGTTATT
  121 AGCAGTTTTT GGAGAGGCAT TGGAATACGT AAACATTGGT TTAAGTCATT TCTTGGCTTT
  181 ACCATTGGCC CAAAGAATCT CTTTGATCAT AATAATTCCT TTCATTTACA ATATTGTATG
  241 GCAATTACTA TATTCTTTGA GAAAGGACCG TCCACCTCTA GTGTTTTACT GGATTCCATG
  301 GGTCGGTAGT GCTGTTGTGT ACGGTATGAA GCCATACGAG TTTTTCGAAG AATGTCAAAA
  361 GAAATACGGT GATATTTTTT CATTCGTTTT GTTAGGAAGA GTCATGACTG TGTATTTAGG
  421 ACCAAAGGGT CACGAATTTG TCTTCAACGC TAAGTTGGCA GATGTTTCAG CAGAAGCTGC
  481 TTACGCTCAT TTGACTACTC CAGTTTTCGG TAAAGGTGTT ATTTACGATT GTCCAAATTC
  541 TAGATTGATG GAGCAAAAGA AGTTTGTTAA GGGTGCTCTA ACCAAAGAAG CCTTCAAGAG
  601 CTACGTTCCA TTGATTGCTG AAGAAGTGTA CAAGTACTTC AGAGACTCCA AAAACTTCCG
  661 TTTGAATGAA AGAACTACTG GTACTATTGA CGTGATGGTT ACTCAACCTG AAATGACTAT
  721 TTTCACCGCT TCAAGATCAT TATTGGGTAA GGAAATGAGA GCAAAATTGG ATACCGATTT
  781 TGCTTACTTG TACAGTGATT TGGATAAGGG TTTCACTCCA ATCAACTTCG TCTTCCCTAA
  841 CTTACCATTG GAACACTATA GAAAGAGAGA TCACGCTCAA AAGGCTATCT CCGGTACTTA
  901 CATGTCTTTG ATTAAGGAAA GAAGAAAAGA ACAACGACAT TCAAGACAGA GATTTGATCG
  961 ATTCCTTGAT GAAGAACTCT ACCTACAAGG ATGGTGTGAA GATGACTGAT CAAGAATCGC
 1021 TAACTTGTTA ATTGATGTCT TAATGGGTGG TCAACATACT TCTGCTGCCA CTTCTGCTTG
 1081 GATTTTGTTG CACTTGCTGA AAGACCAGAT GTTCCAACAA GGATGTACGA GAACAATGCT
1141 GTTTTGGATG TTGTAGAAGG ATGACTACGA TTATACAGGA AATGGTGAC
//
Region: insert 3' | Primer: Reverse2 | Sequence: RDB16757_A8K1b.seq
>D05837A5_A8K1_3_reverse2_G02_20_ABI24.ab1
    1 AAGGACAATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGGT
   61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTT TAGATCTTTT GTTCTGGATT
  121 TCTCTTTTCC CAGATGATCT TGGCTGGACC GGTTGGAAGA GTAACCATAG ATGTAAAGTC
  181 AGGAGGTGGA ACGGTCTTAC CCTCTGGGTA ATGCCATTTT AATGTTCTGA TAAAAATGGA
  241 CATTAGAACA CCTAGCTGAC AGTAAGCAAA GTGTTCACCG ATACATCTGT GTCTACCACC
  301 ACCGAAAGGT AAGTATGGAG AGCTGACACC CTTAGAAATG GCACCGAAAC CGTAATCGAC
  361 TTCTTCACCG ACGGAATAAG AGGAGGCAGA ATCTTTGTTC CAACGGTGAA TGTTGAATTG
  421 GTGAGCATTA GGGAAGTATT CGTCTCTTAA ATGAGTGTAA CCTGGAGAAA CCAAAACGTG
  481 ATAACCTGCT GGGATGACAT AAGAAGTGTT TGGAACGTGC ATATCTTTCA TAACCTTACG
  541 GAACAAAGAG TGCAATGGAT GGTGCATTCT TAGAGTTTCC TTAATAGTTT GGTTCAACAA
  601 TGGCATTTCT TGTAATAAAT CGTAGGTCAA TTCCTTCTTA CCACCATCCA AAACACGCAT
  661 TTGTTCTTCG TACAATTCTT GTTGGACATC TGGTCTTTCA GCCAAGTGCA ACAAAATCCA
  721 AGCAGAAGTG GCAGCAGAAG TATGTTGACC ACCCATTAAG ACATCAATTA ACAAGTTAGC
  781 GATTTCTTGA TCAGTCATCT TCACACCATC CTTGTAGGTA GAGTTCTTCA TCAAGGAATC
  841 GATCAAATCT CTGTCTTGAA TGTCGTTGTT CTTTCTTCTT TCCTTAATCA AAGACATGTA
  901 AGTACCGGAG ATAGCCTTTT GAGCGTGATC TCTCTTTCTA TAGTGTTCCA ATGGTAAGTT
  961 AGGGAAGACG AAGTTGATTG GAGTGAAACC CTTATCCAAA TCACTGTACA AGTAAGCAAA
 1021 ATCGGTATCC AATTTTGCTC TCATTTCCTT TACCCAATAA TGATCTGAGC GGTGAAAATA
 1081 GTCATTTCAG CTGAGTACAT CACGTCATAG TTACCAGTAG TCTTTCATCC AACAAAGTTT
1141 TGAGTCTCTT GAAGTACTGA ACCGTCTCAG CATTCATGTG TGAC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ishida, N., A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS. Biochem. Biophys. Res. Commun. 155 (1): 317-323 (1988). PMID 3046615.

2019.08.23

GNP_filter3_RDBDEP_html_190816.pl