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Yeast mutant CYP51F1m/pBKS- (#RDB16757)

Cloning vector of G310D variant of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.

Clone info. Cloning vector of G310D variant of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.
Comment Modified to ATGGCTCTCT TATTAGCAGT TTTT from ATGTCTGCTA CCAAGTCAAT CGTT of nucleic acid sequence coding N-terminal 10 amino acid residues.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Saccharomyces cerevisiae mutated CYP51F1(erg11) cDNA
Depositor|Developer Aoyama, Yuri |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ishida, N et al. Biochem. Biophys. Res. Commun. 155(1), 317-23, 1988) is requested.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ishida, N et al. Biochem. Biophys. Res. Commun. 155(1), 317-23, 1988)の引用を必要とする。

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RDB16757 Yeast mutant CYP51F1m/pBKS- DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Yeast mutant CYP51F1m/pBKS- was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16757).

Reference section:

Ishida, N., Aoyama, Y., Hatanaka, R., Oyama, Y., Imajo, S., Ishiguro, M., Oshima, T., Nakazato, H., Noguchi, T., Maitra, U.S., et al., A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS. Biochem. Biophys. Res. Commun. 155 (1): 317-323 (1988). PMID 3046615. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16757_A8K1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Region: insert 5'
Sequence file: RDB16757_A8K1a.seq check
>D05837A5_A8K1_3_M13_-40_F02_17_ABI24.ab1
    1 AAGTCAGCGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CACCGCGGTG
   61 GCGGCCGCTC TAGAACTAGT GGATCCATCG ATGCTTAGGA GGTCATATGG CTCTGTTATT
  121 AGCAGTTTTT GGAGAGGCAT TGGAATACGT AAACATTGGT TTAAGTCATT TCTTGGCTTT
  181 ACCATTGGCC CAAAGAATCT CTTTGATCAT AATAATTCCT TTCATTTACA ATATTGTATG
  241 GCAATTACTA TATTCTTTGA GAAAGGACCG TCCACCTCTA GTGTTTTACT GGATTCCATG
  301 GGTCGGTAGT GCTGTTGTGT ACGGTATGAA GCCATACGAG TTTTTCGAAG AATGTCAAAA
  361 GAAATACGGT GATATTTTTT CATTCGTTTT GTTAGGAAGA GTCATGACTG TGTATTTAGG
  421 ACCAAAGGGT CACGAATTTG TCTTCAACGC TAAGTTGGCA GATGTTTCAG CAGAAGCTGC
  481 TTACGCTCAT TTGACTACTC CAGTTTTCGG TAAAGGTGTT ATTTACGATT GTCCAAATTC
  541 TAGATTGATG GAGCAAAAGA AGTTTGTTAA GGGTGCTCTA ACCAAAGAAG CCTTCAAGAG
  601 CTACGTTCCA TTGATTGCTG AAGAAGTGTA CAAGTACTTC AGAGACTCCA AAAACTTCCG
  661 TTTGAATGAA AGAACTACTG GTACTATTGA CGTGATGGTT ACTCAACCTG AAATGACTAT
  721 TTTCACCGCT TCAAGATCAT TATTGGGTAA GGAAATGAGA GCAAAATTGG ATACCGATTT
  781 TGCTTACTTG TACAGTGATT TGGATAAGGG TTTCACTCCA ATCAACTTCG TCTTCCCTAA
  841 CTTACCATTG GAACACTATA GAAAGAGAGA TCACGCTCAA AAGGCTATCT CCGGTACTTA
  901 CATGTCTTTG ATTAAGGAAA GAAGAAAAGA ACAACGACAT TCAAGACAGA GATTTGATCG
  961 ATTCCTTGAT GAAGAACTCT ACCTACAAGG ATGGTGTGAA GATGACTGAT CAAGAATCGC
 1021 TAACTTGTTA ATTGATGTCT TAATGGGTGG TCAACATACT TCTGCTGCCA CTTCTGCTTG
 1081 GATTTTGTTG CACTTGCTGA AAGACCAGAT GTTCCAACAA GGATGTACGA GAACAATGCT
1141 GTTTTGGATG TTGTAGAAGG ATGACTACGA TTATACAGGA AATGGTGAC
//
Primer: Reverse2 ()
Region: insert 3'
Sequence file: RDB16757_A8K1b.seq check
>D05837A5_A8K1_3_reverse2_G02_20_ABI24.ab1
    1 AAGGACAATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGGT
   61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTT TAGATCTTTT GTTCTGGATT
  121 TCTCTTTTCC CAGATGATCT TGGCTGGACC GGTTGGAAGA GTAACCATAG ATGTAAAGTC
  181 AGGAGGTGGA ACGGTCTTAC CCTCTGGGTA ATGCCATTTT AATGTTCTGA TAAAAATGGA
  241 CATTAGAACA CCTAGCTGAC AGTAAGCAAA GTGTTCACCG ATACATCTGT GTCTACCACC
  301 ACCGAAAGGT AAGTATGGAG AGCTGACACC CTTAGAAATG GCACCGAAAC CGTAATCGAC
  361 TTCTTCACCG ACGGAATAAG AGGAGGCAGA ATCTTTGTTC CAACGGTGAA TGTTGAATTG
  421 GTGAGCATTA GGGAAGTATT CGTCTCTTAA ATGAGTGTAA CCTGGAGAAA CCAAAACGTG
  481 ATAACCTGCT GGGATGACAT AAGAAGTGTT TGGAACGTGC ATATCTTTCA TAACCTTACG
  541 GAACAAAGAG TGCAATGGAT GGTGCATTCT TAGAGTTTCC TTAATAGTTT GGTTCAACAA
  601 TGGCATTTCT TGTAATAAAT CGTAGGTCAA TTCCTTCTTA CCACCATCCA AAACACGCAT
  661 TTGTTCTTCG TACAATTCTT GTTGGACATC TGGTCTTTCA GCCAAGTGCA ACAAAATCCA
  721 AGCAGAAGTG GCAGCAGAAG TATGTTGACC ACCCATTAAG ACATCAATTA ACAAGTTAGC
  781 GATTTCTTGA TCAGTCATCT TCACACCATC CTTGTAGGTA GAGTTCTTCA TCAAGGAATC
  841 GATCAAATCT CTGTCTTGAA TGTCGTTGTT CTTTCTTCTT TCCTTAATCA AAGACATGTA
  901 AGTACCGGAG ATAGCCTTTT GAGCGTGATC TCTCTTTCTA TAGTGTTCCA ATGGTAAGTT
  961 AGGGAAGACG AAGTTGATTG GAGTGAAACC CTTATCCAAA TCACTGTACA AGTAAGCAAA
 1021 ATCGGTATCC AATTTTGCTC TCATTTCCTT TACCCAATAA TGATCTGAGC GGTGAAAATA
 1081 GTCATTTCAG CTGAGTACAT CACGTCATAG TTACCAGTAG TCTTTCATCC AACAAAGTTT
1141 TGAGTCTCTT GAAGTACTGA ACCGTCTCAG CATTCATGTG TGAC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ishida, N., A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS. Biochem. Biophys. Res. Commun. 155 (1): 317-323 (1988). PMID 3046615. [link to RRC of NBRP]