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Yeast CYP51F1(erg11)m/pBKS-

Cloning vector of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.

Catalog number RDB16756
Resource name Yeast CYP51F1(erg11)m/pBKS-
Clone info. Cloning vector of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.
Modified to ATGGCTCTCT TATTAGCAGT TTTT from ATGTCTGCTA CCAAGTCAAT CGTT of nucleic acid sequence coding N-terminal 10 amino acid residues.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Saccharomyces cerevisiae CYP51F1(erg11) cDNA
Depositor Aoyama, Yuri |
 
Other clones in our bank Saccharomyces cerevisiae ERG11 (KEGG orthology K05917) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kitahama, Y et al. Biol. Pharm Bull. 32(4), 558-63, 2009) is requested.
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RDB16756 Yeast CYP51F1(erg11)m/pBKS- DNA solution

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References and tips

Electronic file

Original reference

original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16756_A8K1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: insert 5' | Primer: M13_-40 | Sequence: RDB16756_A8K1a.seq
>D05837A4_A8K1_1_M13_-40_D02_11_ABI24.ab1
    1 AAGCCCCAGG GAACCGCGGC GGTAATACGA CTCACTATAG GGCGAATTGG AGCTCCACCG
   61 CGGTGGCGGC CGCTCTAGAA CTAGTGGATC CATCGATGCT TAGGAGGTCA TATGGCTCTG
  121 TTATTAGCAG TTTTTGGAGA GGCATTGGAA TACGTAAACA TTGGTTTAAG TCATTTCTTG
  181 GCTTTACCAT TGGCCCAAAG AATCTCTTTG ATCATAATAA TTCCTTTCAT TTACAATATT
  241 GTATGGCAAT TACTATATTC TTTGAGAAAG GACCGTCCAC CTCTAGTGTT TTACTGGATT
  301 CCATGGGTCG GTAGTGCTGT TGTGTACGGT ATGAAGCCAT ACGAGTTTTT CGAAGAATGT
  361 CAAAAGAAAT ACGGTGATAT TTTTTCATTC GTTTTGTTAG GAAGAGTCAT GACTGTGTAT
  421 TTAGGACCAA AGGGTCACGA ATTTGTCTTC AACGCTAAGT TGGCAGATGT TTCAGCAGAA
  481 GCTGCTTACG CTCATTTGAC TACTCCAGTT TTCGGTAAAG GTGTTATTTA CGATTGTCCA
  541 AATTCTAGAT TGATGGAGCA AAAGAAGTTT GTTAAGGGTG CTCTAACCAA AGAAGCCTTC
  601 AAGAGCTACG TTCCATTGAT TGCTGAAGAA GTGTACAAGT ACTTCAGAGA CTCCAAAAAC
  661 TTCCGTTTGA ATGAAAGAAC TACTGGTACT ATTGACGTGA TGGTTACTCA ACCTGAAATG
  721 ACTATTTTCA CCGCTTCAAG ATCATTATTG GGTAAGGAAA TGAGAGCAAA ATTGGATACC
  781 GATTTTGCTT ACTTGTACAG TGATTTGGAT AAGGGTTTCA CTCCAATCAA CTTCGTCTTC
  841 CCTAACTTAC CATTGGAACA CTATAGAAAG AGAGATCACG CTCAAAAGGC TATCTCCGGT
  901 ACTTACATGT CTTTGATTAA AGGAAAGAAG AAAGAACAAC GACATTCAAG ACAGAGATTT
  961 GATCGATTCC TTGATGAAAG AACTCTACCT ACAAGGATGG TGTGAAGATG ACTGATCAAG
 1021 AAATCGCTAA CTTGTTTAAT TGGTGTCTTA ATGGGTGGGT CAACATACTT CTGCTGCCAC
 1081 TTCTGCTTGG GATTTTGTTG CACTTGGCTG AAAGACCAGG AATGTCCACA GATGTACGAA
1141 GAACAAATGC CTGTTTTTGA TGGGTGGTAA GAAAGGGAAA TTGA
//
Region: insert 3' | Primer: Reverse2 | Sequence: RDB16756_A8K1b.seq
>D05837A4_A8K1_1_reverse2_E02_14_ABI24.ab1
    1 AAGGTACATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGGT
   61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTT TAGATCTTTT GTTCTGGATT
  121 TCTCTTTTCC CAGATGATCT TGGCTGGACC GGTTGGAAGA GTAACCATAG ATGTAAAGTC
  181 AGGAGGTGGA ACGGTCTTAC CCTCTGGGTA ATGCCATTTT AATGTTCTGA TAAAAATGGA
  241 CATTAGAACA CCTAGCTGAC AGTAAGCAAA GTGTTCACCG ATACATCTGT GTCTACCACC
  301 ACCGAAAGGT AAGTATGGAG AGCTGACACC CTTAGAAATG GCACCGAAAC CGTAATCGAC
  361 TTCTTCACCG ACGGAATAAG AGGAGGCAGA ATCTTTGTTC CAACGGTGAA TGTTGAATTG
  421 GTGAGCATTA GGGAAGTATT CGTCTCTTAA ATGAGTGTAA CCTGGAGAAA CCAAAACGTG
  481 ATAACCTGCT GGGATGACAT AAGAAGTGTT TGGAACGTGC ATATCTTTCA TAACCTTACG
  541 GAACAAAGAG TGCAATGGAT GGTGCATTCT TAGAGTTTCC TTAATAGTTT GGTTCAACAA
  601 TGGCATTTCT TGTAATAAAT CGTAGGTCAA TTCCTTCTTA CCACCATCCA AAACACGCAT
  661 TTGTTCTTCG TACAATTCTT GTTGGACATC TGGTCTTTCA GCCAAGTGCA ACAAAATCCA
  721 AGCAGAAGTG GCAGCAGAAG TATGTTGACC ACCCATTAAG ACACCAATTA ACAAGTTAGC
  781 GATTTCTTGA TCAGTCATCT TCACACCATC CTTGTAGGTA GAGTTCTTCA TCAAGGAATC
  841 GATCAAATCT CTGTCTTGAA TGTCGTTGTT CTTTCTTCTT TCCTTAATCA AAGACATGTA
  901 AGTACCGGAG ATAGCCTTTT GAGCGTGATC TCTCTTTCTA TAGTGTTCCA ATGGTAAGTT
  961 AGGGAAGACG AAGTTGATTG GAGTGAAACC CTTTATCCAA ATCACTGTAC AAGTAAGCAA
 1021 AATCGGTATC CAATTTTGCT CTCATTTCCC TTACCCAATA ATGATCTGAA GCGTGAAAAA
 1081 TAGTCATTTC AGTGAGTAAC ATCACGTCAA TAGTACAGTA GGTTCTTTCC ATCCAACCGA
1141 AGTTTTGAGT CTCTGAAGTA CTGAACCATC TCAGCAAATC TCAAATG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884.

2019.08.23

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