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Yeast CYP51F1(erg11)m/pBKS- (#RDB16756)

Cloning vector of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.

Clone info. Cloning vector of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.
Comment Modified to ATGGCTCTCT TATTAGCAGT TTTT from ATGTCTGCTA CCAAGTCAAT CGTT of nucleic acid sequence coding N-terminal 10 amino acid residues.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Saccharomyces cerevisiae CYP51F1(erg11) cDNA
Depositor|Developer Aoyama, Yuri |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kitahama, Y et al. Biol. Pharm Bull. 32(4), 558-63, 2009) is requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Kitahama, Y et al. Biol. Pharm Bull. 32(4), 558-63, 2009）の引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16756 Yeast CYP51F1(erg11)m/pBKS- DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Yeast CYP51F1(erg11)m/pBKS- was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16756).

Reference section:

Kitahama, Y., Nakamura, M., Yoshida, Y., Aoyama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16756_A8K1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Region: insert 5'
Sequence file: RDB16756_A8K1a.seq check
>D05837A4_A8K1_1_M13_-40_D02_11_ABI24.ab1
    1 AAGCCCCAGG GAACCGCGGC GGTAATACGA CTCACTATAG GGCGAATTGG AGCTCCACCG
   61 CGGTGGCGGC CGCTCTAGAA CTAGTGGATC CATCGATGCT TAGGAGGTCA TATGGCTCTG
  121 TTATTAGCAG TTTTTGGAGA GGCATTGGAA TACGTAAACA TTGGTTTAAG TCATTTCTTG
  181 GCTTTACCAT TGGCCCAAAG AATCTCTTTG ATCATAATAA TTCCTTTCAT TTACAATATT
  241 GTATGGCAAT TACTATATTC TTTGAGAAAG GACCGTCCAC CTCTAGTGTT TTACTGGATT
  301 CCATGGGTCG GTAGTGCTGT TGTGTACGGT ATGAAGCCAT ACGAGTTTTT CGAAGAATGT
  361 CAAAAGAAAT ACGGTGATAT TTTTTCATTC GTTTTGTTAG GAAGAGTCAT GACTGTGTAT
  421 TTAGGACCAA AGGGTCACGA ATTTGTCTTC AACGCTAAGT TGGCAGATGT TTCAGCAGAA
  481 GCTGCTTACG CTCATTTGAC TACTCCAGTT TTCGGTAAAG GTGTTATTTA CGATTGTCCA
  541 AATTCTAGAT TGATGGAGCA AAAGAAGTTT GTTAAGGGTG CTCTAACCAA AGAAGCCTTC
  601 AAGAGCTACG TTCCATTGAT TGCTGAAGAA GTGTACAAGT ACTTCAGAGA CTCCAAAAAC
  661 TTCCGTTTGA ATGAAAGAAC TACTGGTACT ATTGACGTGA TGGTTACTCA ACCTGAAATG
  721 ACTATTTTCA CCGCTTCAAG ATCATTATTG GGTAAGGAAA TGAGAGCAAA ATTGGATACC
  781 GATTTTGCTT ACTTGTACAG TGATTTGGAT AAGGGTTTCA CTCCAATCAA CTTCGTCTTC
  841 CCTAACTTAC CATTGGAACA CTATAGAAAG AGAGATCACG CTCAAAAGGC TATCTCCGGT
  901 ACTTACATGT CTTTGATTAA AGGAAAGAAG AAAGAACAAC GACATTCAAG ACAGAGATTT
  961 GATCGATTCC TTGATGAAAG AACTCTACCT ACAAGGATGG TGTGAAGATG ACTGATCAAG
 1021 AAATCGCTAA CTTGTTTAAT TGGTGTCTTA ATGGGTGGGT CAACATACTT CTGCTGCCAC
 1081 TTCTGCTTGG GATTTTGTTG CACTTGGCTG AAAGACCAGG AATGTCCACA GATGTACGAA
1141 GAACAAATGC CTGTTTTTGA TGGGTGGTAA GAAAGGGAAA TTGA
//
Primer: Reverse2 ()
Region: insert 3'
Sequence file: RDB16756_A8K1b.seq check
>D05837A4_A8K1_1_reverse2_E02_14_ABI24.ab1
    1 AAGGTACATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGGT
   61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTT TAGATCTTTT GTTCTGGATT
  121 TCTCTTTTCC CAGATGATCT TGGCTGGACC GGTTGGAAGA GTAACCATAG ATGTAAAGTC
  181 AGGAGGTGGA ACGGTCTTAC CCTCTGGGTA ATGCCATTTT AATGTTCTGA TAAAAATGGA
  241 CATTAGAACA CCTAGCTGAC AGTAAGCAAA GTGTTCACCG ATACATCTGT GTCTACCACC
  301 ACCGAAAGGT AAGTATGGAG AGCTGACACC CTTAGAAATG GCACCGAAAC CGTAATCGAC
  361 TTCTTCACCG ACGGAATAAG AGGAGGCAGA ATCTTTGTTC CAACGGTGAA TGTTGAATTG
  421 GTGAGCATTA GGGAAGTATT CGTCTCTTAA ATGAGTGTAA CCTGGAGAAA CCAAAACGTG
  481 ATAACCTGCT GGGATGACAT AAGAAGTGTT TGGAACGTGC ATATCTTTCA TAACCTTACG
  541 GAACAAAGAG TGCAATGGAT GGTGCATTCT TAGAGTTTCC TTAATAGTTT GGTTCAACAA
  601 TGGCATTTCT TGTAATAAAT CGTAGGTCAA TTCCTTCTTA CCACCATCCA AAACACGCAT
  661 TTGTTCTTCG TACAATTCTT GTTGGACATC TGGTCTTTCA GCCAAGTGCA ACAAAATCCA
  721 AGCAGAAGTG GCAGCAGAAG TATGTTGACC ACCCATTAAG ACACCAATTA ACAAGTTAGC
  781 GATTTCTTGA TCAGTCATCT TCACACCATC CTTGTAGGTA GAGTTCTTCA TCAAGGAATC
  841 GATCAAATCT CTGTCTTGAA TGTCGTTGTT CTTTCTTCTT TCCTTAATCA AAGACATGTA
  901 AGTACCGGAG ATAGCCTTTT GAGCGTGATC TCTCTTTCTA TAGTGTTCCA ATGGTAAGTT
  961 AGGGAAGACG AAGTTGATTG GAGTGAAACC CTTTATCCAA ATCACTGTAC AAGTAAGCAA
 1021 AATCGGTATC CAATTTTGCT CTCATTTCCC TTACCCAATA ATGATCTGAA GCGTGAAAAA
 1081 TAGTCATTTC AGTGAGTAAC ATCACGTCAA TAGTACAGTA GGTTCTTTCC ATCCAACCGA
1141 AGTTTTGAGT CTCTGAAGTA CTGAACCATC TCAGCAAATC TCAAATG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884. [link to RRC of NBRP]