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Yeast CYP51F1(erg11)m/pBKS-

Cloning vector of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.

Catalog number RDB16756
Resource name Yeast CYP51F1(erg11)m/pBKS-
Clone info. Cloning vector of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.
Comment Modified to ATGGCTCTCT TATTAGCAGT TTTT from ATGTCTGCTA CCAAGTCAAT CGTT of nucleic acid sequence coding N-terminal 10 amino acid residues.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Saccharomyces cerevisiae CYP51F1(erg11) cDNA
Depositor|Developer Aoyama, Yuri |
Other clones in our bank
External Database
Saccharomyces cerevisiae CYP51F1(erg11)
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kitahama, Y et al. Biol. Pharm Bull. 32(4), 558-63, 2009) is requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Kitahama, Y et al. Biol. Pharm Bull. 32(4), 558-63, 2009）の引用を必要とする。
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          RDB16756 Yeast CYP51F1(erg11)m/pBKS- DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16756_A8K1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Region: insert 5'
          Sequence file: RDB16756_A8K1a.seq check
          >D05837A4_A8K1_1_M13_-40_D02_11_ABI24.ab1
              1 AAGCCCCAGG GAACCGCGGC GGTAATACGA CTCACTATAG GGCGAATTGG AGCTCCACCG
             61 CGGTGGCGGC CGCTCTAGAA CTAGTGGATC CATCGATGCT TAGGAGGTCA TATGGCTCTG
            121 TTATTAGCAG TTTTTGGAGA GGCATTGGAA TACGTAAACA TTGGTTTAAG TCATTTCTTG
            181 GCTTTACCAT TGGCCCAAAG AATCTCTTTG ATCATAATAA TTCCTTTCAT TTACAATATT
            241 GTATGGCAAT TACTATATTC TTTGAGAAAG GACCGTCCAC CTCTAGTGTT TTACTGGATT
            301 CCATGGGTCG GTAGTGCTGT TGTGTACGGT ATGAAGCCAT ACGAGTTTTT CGAAGAATGT
            361 CAAAAGAAAT ACGGTGATAT TTTTTCATTC GTTTTGTTAG GAAGAGTCAT GACTGTGTAT
            421 TTAGGACCAA AGGGTCACGA ATTTGTCTTC AACGCTAAGT TGGCAGATGT TTCAGCAGAA
            481 GCTGCTTACG CTCATTTGAC TACTCCAGTT TTCGGTAAAG GTGTTATTTA CGATTGTCCA
            541 AATTCTAGAT TGATGGAGCA AAAGAAGTTT GTTAAGGGTG CTCTAACCAA AGAAGCCTTC
            601 AAGAGCTACG TTCCATTGAT TGCTGAAGAA GTGTACAAGT ACTTCAGAGA CTCCAAAAAC
            661 TTCCGTTTGA ATGAAAGAAC TACTGGTACT ATTGACGTGA TGGTTACTCA ACCTGAAATG
            721 ACTATTTTCA CCGCTTCAAG ATCATTATTG GGTAAGGAAA TGAGAGCAAA ATTGGATACC
            781 GATTTTGCTT ACTTGTACAG TGATTTGGAT AAGGGTTTCA CTCCAATCAA CTTCGTCTTC
            841 CCTAACTTAC CATTGGAACA CTATAGAAAG AGAGATCACG CTCAAAAGGC TATCTCCGGT
            901 ACTTACATGT CTTTGATTAA AGGAAAGAAG AAAGAACAAC GACATTCAAG ACAGAGATTT
            961 GATCGATTCC TTGATGAAAG AACTCTACCT ACAAGGATGG TGTGAAGATG ACTGATCAAG
           1021 AAATCGCTAA CTTGTTTAAT TGGTGTCTTA ATGGGTGGGT CAACATACTT CTGCTGCCAC
           1081 TTCTGCTTGG GATTTTGTTG CACTTGGCTG AAAGACCAGG AATGTCCACA GATGTACGAA
          1141 GAACAAATGC CTGTTTTTGA TGGGTGGTAA GAAAGGGAAA TTGA
          //
          Primer: Reverse2
          Region: insert 3'
          Sequence file: RDB16756_A8K1b.seq check
          >D05837A4_A8K1_1_reverse2_E02_14_ABI24.ab1
              1 AAGGTACATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGGT
             61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTT TAGATCTTTT GTTCTGGATT
            121 TCTCTTTTCC CAGATGATCT TGGCTGGACC GGTTGGAAGA GTAACCATAG ATGTAAAGTC
            181 AGGAGGTGGA ACGGTCTTAC CCTCTGGGTA ATGCCATTTT AATGTTCTGA TAAAAATGGA
            241 CATTAGAACA CCTAGCTGAC AGTAAGCAAA GTGTTCACCG ATACATCTGT GTCTACCACC
            301 ACCGAAAGGT AAGTATGGAG AGCTGACACC CTTAGAAATG GCACCGAAAC CGTAATCGAC
            361 TTCTTCACCG ACGGAATAAG AGGAGGCAGA ATCTTTGTTC CAACGGTGAA TGTTGAATTG
            421 GTGAGCATTA GGGAAGTATT CGTCTCTTAA ATGAGTGTAA CCTGGAGAAA CCAAAACGTG
            481 ATAACCTGCT GGGATGACAT AAGAAGTGTT TGGAACGTGC ATATCTTTCA TAACCTTACG
            541 GAACAAAGAG TGCAATGGAT GGTGCATTCT TAGAGTTTCC TTAATAGTTT GGTTCAACAA
            601 TGGCATTTCT TGTAATAAAT CGTAGGTCAA TTCCTTCTTA CCACCATCCA AAACACGCAT
            661 TTGTTCTTCG TACAATTCTT GTTGGACATC TGGTCTTTCA GCCAAGTGCA ACAAAATCCA
            721 AGCAGAAGTG GCAGCAGAAG TATGTTGACC ACCCATTAAG ACACCAATTA ACAAGTTAGC
            781 GATTTCTTGA TCAGTCATCT TCACACCATC CTTGTAGGTA GAGTTCTTCA TCAAGGAATC
            841 GATCAAATCT CTGTCTTGAA TGTCGTTGTT CTTTCTTCTT TCCTTAATCA AAGACATGTA
            901 AGTACCGGAG ATAGCCTTTT GAGCGTGATC TCTCTTTCTA TAGTGTTCCA ATGGTAAGTT
            961 AGGGAAGACG AAGTTGATTG GAGTGAAACC CTTTATCCAA ATCACTGTAC AAGTAAGCAA
           1021 AATCGGTATC CAATTTTGCT CTCATTTCCC TTACCCAATA ATGATCTGAA GCGTGAAAAA
           1081 TAGTCATTTC AGTGAGTAAC ATCACGTCAA TAGTACAGTA GGTTCTTTCC ATCCAACCGA
          1141 AGTTTTGAGT CTCTGAAGTA CTGAACCATC TCAGCAAATC TCAAATG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884.

          2022.05.17

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