Sequencing and PCR primers for primer information.
Cloning vector of rat cytochrome P450 family 51 subfamily A member 1 (CYP51A1).
| Catalog number | RDB16754 |
|---|---|
| Resource name | Rat CYP51A1/pBSK- |
| Clone info. | Cloning vector of rat cytochrome P450 family 51 subfamily A member 1 (CYP51A1). |
| Vector backbone | pBluescript SK- (plasmid) |
| Size of vector backbone | 3.0 kb |
| Selectable markers | Amp^r |
| Gene/insert name | Rat CYP51A1 cDNA |
| Depositor | Aoyama, Yuri | |
| Other clones in our bank | Rat Cyp51 (KEGG orthology K05917) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Aoyama, Y. et al. J. Biochem. 119(5), 926-33, 1996) is requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16754 | Rat CYP51A1/pBSK- | DNA solution |
Electronic file
Original reference
| original | Aoyama, Y., Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species. J. Biochem. 119 (5): 926-933 (1996). PMID 8797093. |
|---|
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16754_A8K1p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Region: insert 5' | Primer: M13_-40 | Sequence: RDB16754_A8K1a.seq |
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|
>D05837A2-A8K1_1_M13_-40_C01_03_ABI08.ab1 1 CGGCCGGGTT AAATGTATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG 61 AGGTCGACGG TATCGATAAG CTTGATATCG AATTCGGCGG AGGCCGGGGC CGCCTCCCGA 121 CGCCCACCTA CTAGCTTCTC CAGCTCCATC TTTCCCGAGA AGCGGCAGGG ACCTCGGCCT 181 CCAGCTTACC GGGCGGATCG AAGCAGCGGT CGGGATGGTA CTGCTGGGCT TGCTGCAGTC 241 AGGCGGCTCG GTGCTCGGGC AGGCGATGGA GCAGGTGACA GGAGGCAACC TGCTTTCCAC 301 GCTGCTCATC GCCTGCGCCT TCACGCTTAG CCTTGTCTAC CTGTTCCGCC TCGCAGTGGG 361 CCACATGGTC CAGCTGCCCG CTGGAGCGAA AAGTCCGCCA TATATTTACT CTCCAATTCC 421 GTTCCTTGGC CACGCGATAG CATTTGGGAA AAGTCCAATT GAGTTCCTGG AAAATGCATA 481 TGAGAAGTAC GGACCTGTGT TTAGCTTTAC CATGGTGGGC AAGACTTTCA CTTACCTTTT 541 GGGGAGTGAC GCAGCCGCCC TGCTCTTCAA CAGTAAGAAC GAAGACCTGA ATGCGGAAGA 601 GGTCTACGGT CGGCTGACAA CGCCCGTGTT TGGGAAGGGA GTTGCATACG ACGTGCCAAA 661 TGCAGTTTTC CTGGAACAGA AGAAAATACT AAAAAGCGGC CTTAACATAG CCCACTTCAA 721 GCAGTATGTT TCTATCATTG AAAAAGAAGC GAAGGAGTAC TTTAAAAGTT GGGGAGAAAG 781 CGGAGAAAGA AATGTGTTTG AAGCGCTGTC TGAGCTCATA ATTTTAACAG CTAGCCATTG 841 CTTACATGGA AAGGAAATTA GAAGTCAGCT CAACGAGAAA GTGGCTCAGC TGTACGCAGA 901 CCTGGGATGG AGGCTTTAGC CATGCCGCCT GGGCTGTTGC CAGGCTGGCT GCCCCTGCCC 961 GAGTTTCAGG CGCAGGACAG GAGCCCAATC GGAGAGATCA AGAATATTTT TCTACAAGGC 1021 CATCCAGAAC GGTAAGCTGT CAAAGAACTA GGTCGGAGGA CACTCGTCGA AACTCGACTG 1081 GAGTCCTACC GTACAAGGAA TTGGGGG // |
| Region: insert 3' | Primer: Reverse2 | Sequence: RDB16754_A8K1b.seq |
|
>D05837A2-A8K1_1-reverse2_A01_01_ABI08.ab1 1 GTAAACAGGG ATTACGCCAA GCTCGAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC 61 TCCACCGCGG TGGCGGCCGC TCTAGAACTA GTGGATCCCC CGGGCTGCAG GAATTCGGTT 121 TTTTTTTTAA TTAAAATTAA AAATTCCACA AGGATTTGAT TTTGGATTTT TCTGGAACCA 181 ATAACATAAG TACTCTTTAG TATTTATTAT TTTTAGGAAA TAAGGTTATC AAGATAAGGT 241 CCAAGATTGC TGCCATCTCG CTGGAGTGGT GCTCTCAGTT AACACTACAA AGGGCACTCA 301 CACCATCTAG AGCTTCGCAG AAAACTTATC CTTCACCCTC TCACATCTTT ACGCACAGAA 361 GTGGCCCAAC CACACCAATG ACCCATTGCC TCTGTAGCAT CTGACCAGGC TTAAAACTTC 421 CCAAACTAGT TCCAAGCCGG AATCTCCCTA CGGCAACGTG TGCTTCTGAA GTTAGTTACC 481 TGGATGCTAT ATTCTGTATC ATCTCTGAAT ACCGAGGCTC CCCTAAACGA TTTCTAACAT 541 AAACTTGGCT TCCCTGAAAT CCTATCAGAA CTATTATAAC TGTTTCAAAT GCCATTAGGT 601 CTGACAAAAA TCACCGAGTT AATAAAACAT TAAAATCAAA CTGGCTTTCA GAACCACACA 661 CTTAAAAAAC AGTACAGTAT GAAAGCTGTG TCAAAGCTTC ATTCTCTGCC AAGCTGCAGC 721 TTGCAGTCCC GTCTCCACAC TGGCTCCTTG TTCCTTTCTT CATTTTGATC TTCGTTTGTA 781 ACGGATTACT GGGTTTTCTG GGGTATGAAT CATTGTTGTA TAATTCACAC TGGGAAAATA 841 TCCATTGATG AGGTCAAATT CATATAAACG AAGCATAGTG GACCAAATTG TCTTAATTTG 901 AACATAGGCA AAATTTTCTC CAATACAACG ATGGCGCCCG GGCTCCAAAC GGGCACATAG 961 GCAAACTTTC TCTCCCGACG CTGGGGTTGG TCCTGGTAGT AGCGGGTCAG ATTGAAAGTT 1021 CCAAGCGTTC TACCAGAGTC TTTCAGCTCT TTGATGACAG TGGAGACACA CACCTTGAGT 1081 GTCCTGAGAA TGTGTACCTC AGCTC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Aoyama, Y., Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species. J. Biochem. 119 (5): 926-933 (1996). PMID 8797093. |
|---|
2019.08.23
GNP_filter3_RDBDEP_html_190816.pl