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Rat CYP51A1/pBSK-

Cloning vector of rat cytochrome P450 family 51 subfamily A member 1 (CYP51A1).

Catalog number RDB16754
Resource name Rat CYP51A1/pBSK-
Clone info. Cloning vector of rat cytochrome P450 family 51 subfamily A member 1 (CYP51A1).
Vector backbone pBluescript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Rat CYP51A1 cDNA
Depositor Aoyama, Yuri |
 
Other clones in our bank Rat Cyp51 (KEGG orthology K05917) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Aoyama, Y. et al. J. Biochem. 119(5), 926-33, 1996) is requested.
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RDB16754 Rat CYP51A1/pBSK- DNA solution

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References and tips

Electronic file

Original reference

original Aoyama, Y., Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species. J. Biochem. 119 (5): 926-933 (1996). PMID 8797093.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16754_A8K1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: insert 5' | Primer: M13_-40 | Sequence: RDB16754_A8K1a.seq
>D05837A2-A8K1_1_M13_-40_C01_03_ABI08.ab1
    1 CGGCCGGGTT AAATGTATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG
   61 AGGTCGACGG TATCGATAAG CTTGATATCG AATTCGGCGG AGGCCGGGGC CGCCTCCCGA
  121 CGCCCACCTA CTAGCTTCTC CAGCTCCATC TTTCCCGAGA AGCGGCAGGG ACCTCGGCCT
  181 CCAGCTTACC GGGCGGATCG AAGCAGCGGT CGGGATGGTA CTGCTGGGCT TGCTGCAGTC
  241 AGGCGGCTCG GTGCTCGGGC AGGCGATGGA GCAGGTGACA GGAGGCAACC TGCTTTCCAC
  301 GCTGCTCATC GCCTGCGCCT TCACGCTTAG CCTTGTCTAC CTGTTCCGCC TCGCAGTGGG
  361 CCACATGGTC CAGCTGCCCG CTGGAGCGAA AAGTCCGCCA TATATTTACT CTCCAATTCC
  421 GTTCCTTGGC CACGCGATAG CATTTGGGAA AAGTCCAATT GAGTTCCTGG AAAATGCATA
  481 TGAGAAGTAC GGACCTGTGT TTAGCTTTAC CATGGTGGGC AAGACTTTCA CTTACCTTTT
  541 GGGGAGTGAC GCAGCCGCCC TGCTCTTCAA CAGTAAGAAC GAAGACCTGA ATGCGGAAGA
  601 GGTCTACGGT CGGCTGACAA CGCCCGTGTT TGGGAAGGGA GTTGCATACG ACGTGCCAAA
  661 TGCAGTTTTC CTGGAACAGA AGAAAATACT AAAAAGCGGC CTTAACATAG CCCACTTCAA
  721 GCAGTATGTT TCTATCATTG AAAAAGAAGC GAAGGAGTAC TTTAAAAGTT GGGGAGAAAG
  781 CGGAGAAAGA AATGTGTTTG AAGCGCTGTC TGAGCTCATA ATTTTAACAG CTAGCCATTG
  841 CTTACATGGA AAGGAAATTA GAAGTCAGCT CAACGAGAAA GTGGCTCAGC TGTACGCAGA
  901 CCTGGGATGG AGGCTTTAGC CATGCCGCCT GGGCTGTTGC CAGGCTGGCT GCCCCTGCCC
  961 GAGTTTCAGG CGCAGGACAG GAGCCCAATC GGAGAGATCA AGAATATTTT TCTACAAGGC
 1021 CATCCAGAAC GGTAAGCTGT CAAAGAACTA GGTCGGAGGA CACTCGTCGA AACTCGACTG
1081 GAGTCCTACC GTACAAGGAA TTGGGGG
//
Region: insert 3' | Primer: Reverse2 | Sequence: RDB16754_A8K1b.seq
>D05837A2-A8K1_1-reverse2_A01_01_ABI08.ab1
    1 GTAAACAGGG ATTACGCCAA GCTCGAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC
   61 TCCACCGCGG TGGCGGCCGC TCTAGAACTA GTGGATCCCC CGGGCTGCAG GAATTCGGTT
  121 TTTTTTTTAA TTAAAATTAA AAATTCCACA AGGATTTGAT TTTGGATTTT TCTGGAACCA
  181 ATAACATAAG TACTCTTTAG TATTTATTAT TTTTAGGAAA TAAGGTTATC AAGATAAGGT
  241 CCAAGATTGC TGCCATCTCG CTGGAGTGGT GCTCTCAGTT AACACTACAA AGGGCACTCA
  301 CACCATCTAG AGCTTCGCAG AAAACTTATC CTTCACCCTC TCACATCTTT ACGCACAGAA
  361 GTGGCCCAAC CACACCAATG ACCCATTGCC TCTGTAGCAT CTGACCAGGC TTAAAACTTC
  421 CCAAACTAGT TCCAAGCCGG AATCTCCCTA CGGCAACGTG TGCTTCTGAA GTTAGTTACC
  481 TGGATGCTAT ATTCTGTATC ATCTCTGAAT ACCGAGGCTC CCCTAAACGA TTTCTAACAT
  541 AAACTTGGCT TCCCTGAAAT CCTATCAGAA CTATTATAAC TGTTTCAAAT GCCATTAGGT
  601 CTGACAAAAA TCACCGAGTT AATAAAACAT TAAAATCAAA CTGGCTTTCA GAACCACACA
  661 CTTAAAAAAC AGTACAGTAT GAAAGCTGTG TCAAAGCTTC ATTCTCTGCC AAGCTGCAGC
  721 TTGCAGTCCC GTCTCCACAC TGGCTCCTTG TTCCTTTCTT CATTTTGATC TTCGTTTGTA
  781 ACGGATTACT GGGTTTTCTG GGGTATGAAT CATTGTTGTA TAATTCACAC TGGGAAAATA
  841 TCCATTGATG AGGTCAAATT CATATAAACG AAGCATAGTG GACCAAATTG TCTTAATTTG
  901 AACATAGGCA AAATTTTCTC CAATACAACG ATGGCGCCCG GGCTCCAAAC GGGCACATAG
  961 GCAAACTTTC TCTCCCGACG CTGGGGTTGG TCCTGGTAGT AGCGGGTCAG ATTGAAAGTT
 1021 CCAAGCGTTC TACCAGAGTC TTTCAGCTCT TTGATGACAG TGGAGACACA CACCTTGAGT
1081 GTCCTGAGAA TGTGTACCTC AGCTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Aoyama, Y., Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species. J. Biochem. 119 (5): 926-933 (1996). PMID 8797093.

2019.08.23

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