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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-FLAG-mouse p50 (#RDB16743)

Expression vector of mouse nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1) tagged with FLAG.

Clone info. Expression vector of mouse nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1) tagged with FLAG.
Comment This clone contains a cDNA fragment corresponding to the 503 nt to 1600 nt of NM_008689.2. Known difference (at least) : A to G substitution at 1405nt (Thr, synonym).
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Nfkb1 cDNA
Depositor|Developer Maruyama, Takashi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Biol. Chem. 276 (29): 27657-27662, 2001) and an acknowledgment to the DEPOSITOR and Dr. Tatsushi Muta are requested.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 276 (29): 27657-27662, 2001)。利用者は、研究成果の公表にあたって寄託者ならびに牟田達史博士への謝辞の表明を必要とする。

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RDB16743 pcDNA3-FLAG-mouse p50 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-FLAG-mouse p50 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16743).

Reference section:

Yamazaki, S., Muta, T., Takeshige, K., A novel IkappaB protein, IkappaB-zeta, induced by proinflammatory stimuli, negatively regulates nuclear factor-kappaB in the nuclei. J. Biol. Chem. 276 (29): 27657-27662 (2001). PMID 11356851. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16743_A8Ipp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,FLAG,insert 5'
Sequence file: RDB16743_A8Ipa.seq check
>D05839C8-A8Ip_1_CMV_Foward_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTG NNNNNNNGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGCCAC CATGGACTAC AAGGACGACG ATGACAAGAA
  181 GCTTGGTACC GAGCTCGGAT CCATGGCAGA CGATGATCCC TACGGAACTG GGCAAATGTT
  241 TCATTTGAAC ACTGCTTTGA CTCACTCAAT ATTTAATGCA GAATTATATT CACCAGAAAT
  301 ACCACTGTCA ACAGATGGCC CATACCTTCA AATATTAGAG CAACCAAAAC AGAGGGGATT
  361 TCGATTCCGC TATGTGTGTG AAGGCCCATC ACACGGAGGG CTTCCGGGAG CCTCTAGTGA
  421 GAAGAACAAG AAATCCTACC CACAGGTCAA AATTTGCAAC TATGTGGGGC CTGCAAAGGT
  481 TATCGTTCAG TTGGTCACAA ATGGAAAAAA CATCCACCTG CACGCCCACA GCCTGGTGGG
  541 CAAGCACTGT GAGGACGGGG TATGCACCGT AACAGCAGGA CCCAAGGACA TGGTGGTTGG
  601 CTTTGCAAAC CTGGGAATAC TTCATGTGAC TAAGAAAAAG GTATTTGAAA CACTGGAAGC
  661 ACGGATGACA GAGGCGTGTA TTAGGGGCTA TAATCCTGGA CTTCTGGTGC ATTCTGACCT
  721 TGCCTATCTA CAAGCAGAAN GCGGANGAGA NCGGCAACTC ACAGACAGAG AGAANGAGAT
  781 CATCCGCCAG GCAGCCGTGC AGCAGACCAA GGAGATGGAC CTGANCGTGG TGCGCCTCAT
  841 GTTCACAGCC TTCCTCCCTG ACAGCACTGG CAGCTTCANT CGGANNNTGG ANCCTGTGGT
  901 GNCAGANNNC ATCTATNATA GCAAAGCCCN NAATGNATNC NACCNGAAAA TCGTGNNAAT
961 GGNNANNNNA GCNNNTGTNN GNNNGNNNGG NNNNNNNNNT NNNNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA,SP6 pro,insert 3'
Sequence file: RDB16743_A8Ipb.seq check
>D05839C8-A8Ip_1_BGH_rev2_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG GGGNNGGGCN AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGAG CTCTAGCATT TAGGTGACAC TATAGAATAG GGCCCTCTAG
  121 ATGCATGCTC GAGCGGCCGC CAGTGTGATG GATATCTGCA GAATTCCTAC GGCATAAGCT
  181 TCTGGCGTTT CCTTTGCACT TCCTCTTTGT CTTTGATTTC AGGGTAGTAG AGAAAGGGTT
  241 TCGGTTCACT AGTTTCCAGG TCTGATTTCC TCCGAAGCTG AACAAACACG GAAGCTGGCT
  301 TTGTAATGTT GACATCCTTA TACTTTGGCG TTTTGAAGAC AATGGCAAAC TGTCTATGAA
  361 CATCCGTGGG GGAAAAGTCC CCAAATCCTT CCCAAACTCC GCCATTTTCT TCCTCTTCAT
  421 AAAACCGAAT CTGGATGTCA TCTTTCTGAA CCTTGTCACA GAGAAGGTAA ATCTCCTCCC
  481 CTCCCGTCAC ACATCCTGCT GTTCTGTCCA TTCTCACGAT TTTCAGGTTG GATGCATTCG
  541 GGGCTTTGCT ATCATAGATG GCGTCTGACA CCACAGGCTC CAGTCTCCGA GTGAAGCTGC
  601 CAGTGCTGTC AGGGAGGAAG GCTGTGAACA TGAGGCGCAC CACGCTCAGG TCCATCTCCT
  661 TGGTCTGCTG CACGGCTGCC TGGCGGATGA TCTCCTTCTC TCTGTCTGTG AGTTGCCGGT
  721 CTCCTCCGCC TTCTGCTTGT AGATAGGCAA GGTCAGAATG CACCAGAAGT CCAGGANTAT
  781 AGCCCCTAAT ACACGCCTCT GTCATCCGTG CTTCCAGTGT TTCAAATACC TTTTTCTTAN
  841 NCACATGAAG TATTCCCAGG TTTGCAAAGC CNACCACCAT GTCCTTNNGT NCNGCTGTTA
  901 CGGNGCATAC CCCNNCCNCA CAGNGCTNGC CNACNNNNCN NNNGGCNNGC NNGNNNANGN
961 TTTTTNNNTT TNNGANNNNN NNAANNNNNN NNNTNNNNNN NCNNNNNNNN NN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB16743_A8Ipc.seq check
>D05839C8-A8Ip_1_SV40pro_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG NNGNNGNTTT TTTGGAGGCC TAGGCTTTTG
   61 NNNNNAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
  121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
  661 CATGCCCGAC GGCGAGGNTC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  781 CTATCANGAC ATANCGTTGG CTACCCGTGA TATTGCTGAA NAGCTTGGCG GCGAATGGGC
  841 TGACCGCTTC CTCGTGNTTT ACNGNATCGC CGCNNCCNAT TCGCANCGCA TCGCCTTCTA
  901 TCGCCNTNCT TGACNANTNN TTCTGANCNG GGACTCTGGG GGNNNGAAAT GACCNNANNN
  961 ANGCGANNCC CNAANCNNGC NNTNNNNNAG ANTTTNNANT TNCNNNCNNN NNGNCNNTNC
1021 NANNNNNNNN NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yamazaki, S., A novel IkappaB protein, IkappaB-zeta, induced by proinflammatory stimuli, negatively regulates nuclear factor-kappaB in the nuclei. J. Biol. Chem. 276 (29): 27657-27662 (2001). PMID 11356851. [link to RRC of NBRP]