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pcDNA3-FLAG-mouse p65 (#RDB16729)

Expression vector of mouse nuclear factor-kappaB (NF-kappaB) subunit p65 tagged with FLAG.

Clone info. Expression vector of mouse nuclear factor-kappaB (NF-kappaB) subunit p65 tagged with FLAG.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Rela cDNA
Depositor|Developer Maruyama, Takashi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Leukoc. Biol. 98(3), 385-393, 2015) and an acknowledgment to the DEPOSITOR and Dr. Tatsushi Muta are requested.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Leukoc. Biol. 98(3), 385-393, 2015)。利用者は、研究成果の公表にあたって寄託者ならびに牟田達史博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16729 pcDNA3-FLAG-mouse p65 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-FLAG-mouse p65 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16729).

Reference section:

Yamazaki, S., Muta, T., Takeshige, K., A novel IkappaB protein, IkappaB-zeta, induced by proinflammatory stimuli, negatively regulates nuclear factor-kappaB in the nuclei. J. Biol. Chem. 276 (29): 27657-27662 (2001). PMID 11356851. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16729_A8Ipp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, T7 pro, FLAG, insert 5'
Sequence file: RDB16729_A8Ipa.seq check
>D05839B3-A8Ip_2_CMV_Forward_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NATGGGCGGT AGGCGTGTAC GGTGGGAGGT
   61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT
  121 TAATACGACT CACTATAGGG AGACCCAAGC TGCCACCATG GACTACAAGG ACGACGATGA
  181 CAAGAAGCTT ATGGACGATC TGTTTCCCCT CATCTTTCCC TCAGAGCCAG CCCAGGCTTC
  241 TGGGCCTTAT GTGGAGATCA TCGAACAGCC GAAGCAACGG GGCATGCGAT TCCGCTATAA
  301 ATGCGAGGGG CGCTCAGCGG GCAGTATTCC TGGCGAGAGA AGCACAGATA CCACCAAGAC
  361 ACACCCCACC ATCAAGATCA ATGGCTACAC AGGACCAGGA ACAGTTCGAA TCTCCCTGGT
  421 CACCAAGGAT CCACCTCACC GGCCTCATCC ACATGAACTT GTGGGGAAGG ACTGCCGGGA
  481 TGGCTACTAT GAGGCTGACC TCTGCCCAGA CCGCAGTATC CATAGCTTCC AGAACCTGGG
  541 GATCCAGTGT GTGAAGAAGC GAGACCTGGA GCAAGCCATT AGCCAGCGAA TCCAGACCAA
  601 CAATAACCCC TTTCACGTTC CTATAGAGGA GCAGCGCGGG GACTATGACT TGAATGCAGT
  661 GCGCCTCTGC TTCCAGGTGA CAGTGCGGGA CCCAGCAGGC AGGCCCCTCC TCCTGACCCC
  721 TGTCCTCTCA CATCCGATTT TTGATAACCG GGCCCCCAAC ACTGCCGAGC TCAAGATCTG
  781 CCGAGTAAAC CGGAACTCTG GGAGCTGCCT CGGTGGGGAN GAGATCTTCT TGCTGTGCGA
  841 CAAGGTGCAG AAAGAANNAC ATTGAGGTGT ATTTCACGGG ANCNGGCTGG GGAGGCACGA
  901 GGCTCCTTTT CTCAAGCTGA TGTGCATCNN CAAGTGNNCN NTGTGTTCCN NANTCCTNCG
  961 TACGNNNNNC CCAGCCTNCN GNCTNNNNNN NGANNNNCCN NGCNNCNNNN NNNGNNNNNN
1021 NNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA, insert 3'
Sequence file: RDB16729_A8Ipb.seq check
>D05839B3-A8Ip_2_BGH_rev2_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNGGNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGAG CTCTAGCATT TAGGTGACAC TATAGAATAG GGCCCTCTAG
  121 ATGCATGCTC GAGCGGCCGC CAGTGTGATG GATATCTGCA GAATTCTTAG GAGCTGATCT
  181 GACTCAAAAG AGCAGAGAAG TCCATGTCCG CAATGGAGGA GAAGTCTTCA TCTCCGGAGA
  241 GACCATTGGG AAGCCCCGAG GTCCCCAGGG GTGTGGGAGC TGGGTCAGGG GGCCTCTGGG
  301 ACCCTGTCAC CAGGCGAGTT ATAGCTTCAG GGTACTCCAT CAGCATGGGC TCAGCTGTGG
  361 AGTGAGACAT GGACACACCC TGGTTCAGGA GCTGCTGAAA CTCTGAGTTG TCCACAGATG
  421 CCAGGTCTGT GAACACTCCT GGGTCTGTGC TGTTGCCAAG CAAGGCCCCC AAGTCTTCAT
  481 CAGCATCAAA CTGCAGGTGC AGCAGGGCTT CCGACAGCGT GCCTTCCCCA GCCTGGGTGC
  541 TCTTTGGAAC AGGTGCAGAC AGGGACTGGG GAGGACCCGG GGTTAGAACT GGGGCAGGAG
  601 CTGGGGGCTG AGCCAGAGGT ACCATGGCTG AGGAAGGGAC CATGGTCTGG GCAAGGACTG
  661 GGGCAGAGGA CGGTGCTAAG GCCAGGGCCT GGTTTGAGAT CTGCCCTGAT GGTAACAGCA
  721 TGGGGGAAAA CTCATCAAAG TTGATGGTGC TGAGGGATGC TGGGAANGTG TAGGGCTGCG
  781 GGGCTGGCTT GGGGACAGAA GTTGAGTTTC GGGTANGCAC AGCAATACGC CGGGTTGGAN
  841 GNCGGGGTTC AGTTGGTCCA TTGAAAGGAC TCTTCTTCAT GATACTCTTG AAGGTCTCAT
  901 ANGTCCNTTT TNCGCTTCTC TTCNATCCNG NGNCGATCAT CNNTGNCTGG NNAGTACTGG
961 AACNNNNTGG GNNCACNGAN CTCNCGATCA NNNNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori, NeoR
Sequence file: RDB16729_A8Ipc.seq check
>D05839B3-A8Ip_2_SV40pro_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANNNGTGANG ANGCTTTTTT GGANGNCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  781 TCANGACATA GCGTTGGCTA NNCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGNCTGA
  841 NCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CNTTCTATCG
  901 CCNTCTTGAC GANTNNTTCT GANCGGGNNT CNGGGGGNNN NAAATGACNN ANCNAGCNAC
961 GCCNNANCNN NNNTCNNNAN NNTTNGANNN CNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamazaki, S., A novel IkappaB protein, IkappaB-zeta, induced by proinflammatory stimuli, negatively regulates nuclear factor-kappaB in the nuclei. J. Biol. Chem. 276 (29): 27657-27662 (2001). PMID 11356851. [link to RRC of NBRP]