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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCDNA3-FLAG-human TRAF6 (#RDB16722)

Expression vector of human TNF receptor associated factor 6 (TRAF6) tagged with FLAG.

Clone info. Expression vector of human TNF receptor associated factor 6 (TRAF6) tagged with FLAG.
Comment Expression was confirmed by the depositor with immunoprecipitation and western blotting.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r
Gene/insert name human TRAF6 cDNA
Depositor|Developer Maruyama, Takashi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Biol. Chem. 292(42), 17250-17257, 2017) and an acknowledgment to the DEPOSITOR are requested.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 292(42), 17250-17257, 2017)。利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とする。

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RDB16722 pCDNA3-FLAG-human TRAF6 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCDNA3-FLAG-human TRAF6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16722).

Reference section:

Tanishima, M., Takashima, S., Honda, A., Yasuda, D., Tanikawa, T., Ishii, S., MaruYama, T., Identification of optineurin as an interleukin-1 receptor-associated kinase 1-binding protein and its role in regulation of MyD88-dependent signaling. J. Biol. Chem. 292 (42): 17250-17257 (2017). PMID 28882891. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16722_A8Ipp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMVpro,T7 pro,FLAG,insert 5'
Sequence file: RDB16722_A8Ipa.seq check
>D05839A5-2_A8Ip_CMV-Forward_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNGCNAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTG CCACCATGGA CTACAAGGAC GACGATGACA
  181 AGAAGCTTGG TACCGAGCTC GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCAAA
  241 TGAGTCTGCT AAACTGTGAA AACAGCTGTG GATCCAGCCA GTCTGAAAGT GACTGCTGTG
  301 TGGCCATGGC CAGCTCCTGT AGCGCTGTAA CAAAAGATGA TAGTGTGGGT GGAACTGCCA
  361 GCACGGGGAA CCTCTCCAGC TCATTTATGG AGGAGATCCA GGGATATGAT GTAGAGTTTG
  421 ACCCACCCCT GGAAAGCAAG TATGAATGCC CCATCTGCTT GATGGCATTA CGAGAAGCAG
  481 TGCAAACGCC ATGCGGCCAT AGGTTCTGCA AAGCCTGCAT CATAAAATCA ATAAGGGATG
  541 CAGGTCACAA ATGTCCAGTT GACAATGAAA TACTGCTGGA AAATCAACTA TTTCCAGACA
  601 ATTTTGCAAA ACGTGAGATT CTTTCTCTGA TGGTGAAATG TCCAAATGAA GGTTGTTTGC
  661 ACAAGATGGA ACTGAGACAT CTTGAGGATC ATCAAGCACA TTGTGAGTTT GCTCTTATGG
  721 ATTGTCCCCA ATGCCAGCGT CCCTTCNAAA AATTCCATAT TAATATTCAC ATTCTGAANG
  781 ATTGTCCAAG GAGACAGGTT TCTTGTGACA ACTGTGCTGC ATCAATGGCA TTTGAAGATA
  841 AAGAGATCCA TGACCANAAC TGTCCTTTGG CAAATGTCAT CTGTGAATAC TGNNATACNA
  901 TACTCATCAG ANAACAGATG CCTAATCATT ATGATCTAGA CTGCCCNNNN NCCCCNATNC
  961 CNTGCNNNTN NNNNNCTTTT GGTTGNCNTG AAAANNANGC NNANGNNNCN CNNNGNNCNN
1021 NNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: insert 3'
Sequence file: RDB16722_A8Ipb.seq check
>D05839A5-2_A8Ip_BGH_rev2_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGCNNNC AACAGATGGC TGGCAACTAG
   61 AAGGCNNCAG TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATANGG
  121 CCCTCTAGAT GCATGCTCGA GCTATACCCC TGCATCAGTA CTTCGTGGCT GAAAACCCTC
  181 CCTCCGAAGG CTACCCATGT CAAAGCGGGT GGAGACCTCA CAGCGCACTA ATAATGTGTC
  241 ATCCTTAATG AAAGTTCTTT GTCTTAGGGC TTCCAGATGC ATAAAAGTTA CATAGCCAAA
  301 ACCTTTTGGG TTCCGTGGGA TTGTGGGTCG CTGGAAAGCA AGCAGCTCTG GTTTGGCATC
  361 CATTATCTCT TCGTGGTTTT GCCTTACAGG TGCTTCAGAC TGATCAAGAA TTGTAAGGCG
  421 TATTGTACCC TGGAAGGGCC AAGGGAGGTG GCTGTCATAT TCTCCTTGCA TTGTGTGGAC
  481 AAAAAGGGAT ATATAGTTTG CACAGCGCTG AGCAGTCGGT AACTGAAGGT GCAAGCGCAT
  541 GCACAGTTTG TACCCGGGTT TGCCAGTGTA GAATCCAGGG CTATGAATCA CAACAGGTTT
  601 CTCCTCTTCT TGACATTTCA AATGCATTCC AAAGTTGCCA ATCTTCCAAA TATAAATTCC
  661 ATTGCACTGC TGTGCTTCGA TTTCAGCAAC TTTGTCCTCA AGGGTTCGAA TGGTTCGTTT
  721 GAGCTCACTT ACATACATAC TCTGAGTTTC CATTTTAGCA GTCAGCTCCC GGATTTGATG
  781 GTCTTGTCTT ANNANGCGAC CCTCTAACTG GTGAATAGTT TCCTGGAAAT TCCGGANCTC
  841 TGAGANATAC CNNGAGTCGG GTATNACGCT CAAANCTATG AACAGCCTGG GCCNANNNTT
  901 NNNNNTGTGN GACTGGGGTG TTCTCTTGTN NNTGGCGTGC CNNGTNNNTT CCTCTGCNTC
  961 TTTTNCNTGG NNANCNAANG TACTGAATGT GCNNNGNNNN TNNNGGCNNN ANGGCNNNNT
1021 NNNNNNTNAN NNNNANGNNA TNNNNNNNNN N
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR
Sequence file: RDB16722_A8Ipc.seq check
>D05839A5-2_A8Ip_SV40pro_F_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGAN NNGTGAGGAN GNTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA NGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC
  781 ANGACATAGC GTTGGCTANC CGTGATATTG CTGAAGANCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 NNCTTGANGA NTNNTTCTGA NCGGGANTCT GGGGTTCGAA ATGNNCGANN NANCGNNGCC
961 NNANCNGNCN TCNCNANNNT TNNANTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tanishima, M., Identification of optineurin as an interleukin-1 receptor-associated kinase 1-binding protein and its role in regulation of MyD88-dependent signaling. J. Biol. Chem. 292 (42): 17250-17257 (2017). PMID 28882891. [link to RRC of NBRP]