Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCDNA3-FLAG-human TRAF6

Expression vector of human TNF receptor associated factor 6 (TRAF6) tagged with FLAG.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Biol. Chem. 292(42), 17250-17257, 2017) and an acknowledgment to the DEPOSITOR are requested.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 292(42), 17250-17257, 2017)。利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とする。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16722_A8Ipp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMVpro,T7 pro,FLAG,insert 5' Sequence file: RDB16722_A8Ipa.seq
>D05839A5-2_A8Ip_CMV-Forward_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNNGCNAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA   121 ATACGACTCA CTATAGGGAG ACCCAAGCTG CCACCATGGA CTACAAGGAC GACGATGACA   181 AGAAGCTTGG TACCGAGCTC GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCAAA   241 TGAGTCTGCT AAACTGTGAA AACAGCTGTG GATCCAGCCA GTCTGAAAGT GACTGCTGTG   301 TGGCCATGGC CAGCTCCTGT AGCGCTGTAA CAAAAGATGA TAGTGTGGGT GGAACTGCCA   361 GCACGGGGAA CCTCTCCAGC TCATTTATGG AGGAGATCCA GGGATATGAT GTAGAGTTTG   421 ACCCACCCCT GGAAAGCAAG TATGAATGCC CCATCTGCTT GATGGCATTA CGAGAAGCAG   481 TGCAAACGCC ATGCGGCCAT AGGTTCTGCA AAGCCTGCAT CATAAAATCA ATAAGGGATG   541 CAGGTCACAA ATGTCCAGTT GACAATGAAA TACTGCTGGA AAATCAACTA TTTCCAGACA   601 ATTTTGCAAA ACGTGAGATT CTTTCTCTGA TGGTGAAATG TCCAAATGAA GGTTGTTTGC   661 ACAAGATGGA ACTGAGACAT CTTGAGGATC ATCAAGCACA TTGTGAGTTT GCTCTTATGG   721 ATTGTCCCCA ATGCCAGCGT CCCTTCNAAA AATTCCATAT TAATATTCAC ATTCTGAANG   781 ATTGTCCAAG GAGACAGGTT TCTTGTGACA ACTGTGCTGC ATCAATGGCA TTTGAAGATA   841 AAGAGATCCA TGACCANAAC TGTCCTTTGG CAAATGTCAT CTGTGAATAC TGNNATACNA   901 TACTCATCAG ANAACAGATG CCTAATCATT ATGATCTAGA CTGCCCNNNN NCCCCNATNC   961 CNTGCNNNTN NNNNNCTTTT GGTTGNCNTG AAAANNANGC NNANGNNNCN CNNNGNNCNN 1021 NNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: insert 3' Sequence file: RDB16722_A8Ipb.seq
>D05839A5-2_A8Ip_BGH_rev2_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGCNNNC AACAGATGGC TGGCAACTAG    61 AAGGCNNCAG TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATANGG   121 CCCTCTAGAT GCATGCTCGA GCTATACCCC TGCATCAGTA CTTCGTGGCT GAAAACCCTC   181 CCTCCGAAGG CTACCCATGT CAAAGCGGGT GGAGACCTCA CAGCGCACTA ATAATGTGTC   241 ATCCTTAATG AAAGTTCTTT GTCTTAGGGC TTCCAGATGC ATAAAAGTTA CATAGCCAAA   301 ACCTTTTGGG TTCCGTGGGA TTGTGGGTCG CTGGAAAGCA AGCAGCTCTG GTTTGGCATC   361 CATTATCTCT TCGTGGTTTT GCCTTACAGG TGCTTCAGAC TGATCAAGAA TTGTAAGGCG   421 TATTGTACCC TGGAAGGGCC AAGGGAGGTG GCTGTCATAT TCTCCTTGCA TTGTGTGGAC   481 AAAAAGGGAT ATATAGTTTG CACAGCGCTG AGCAGTCGGT AACTGAAGGT GCAAGCGCAT   541 GCACAGTTTG TACCCGGGTT TGCCAGTGTA GAATCCAGGG CTATGAATCA CAACAGGTTT   601 CTCCTCTTCT TGACATTTCA AATGCATTCC AAAGTTGCCA ATCTTCCAAA TATAAATTCC   661 ATTGCACTGC TGTGCTTCGA TTTCAGCAAC TTTGTCCTCA AGGGTTCGAA TGGTTCGTTT   721 GAGCTCACTT ACATACATAC TCTGAGTTTC CATTTTAGCA GTCAGCTCCC GGATTTGATG   781 GTCTTGTCTT ANNANGCGAC CCTCTAACTG GTGAATAGTT TCCTGGAAAT TCCGGANCTC   841 TGAGANATAC CNNGAGTCGG GTATNACGCT CAAANCTATG AACAGCCTGG GCCNANNNTT   901 NNNNNTGTGN GACTGGGGTG TTCTCTTGTN NNTGGCGTGC CNNGTNNNTT CCTCTGCNTC   961 TTTTNCNTGG NNANCNAANG TACTGAATGT GCNNNGNNNN TNNNGGCNNN ANGGCNNNNT 1021 NNNNNNTNAN NNNNANGNNA TNNNNNNNNN N //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,NeoR Sequence file: RDB16722_A8Ipc.seq
>D05839A5-2_A8Ip_SV40pro_F_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGAN NNGTGAGGAN GNTTTTTTGG AGGCCTAGGC TTTTGCAAAA    61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT   121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC   181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC   241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG   301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG   361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG   421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG   481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC   541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG   601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC   661 CCGACGGCGA NGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG   721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC   781 ANGACATAGC GTTGGCTANC CGTGATATTG CTGAAGANCT TGGCGGCGAA TGGGCTGACC   841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC   901 NNCTTGANGA NTNNTTCTGA NCGGGANTCT GGGGTTCGAA ATGNNCGANN NANCGNNGCC 961 NNANCNGNCN TCNCNANNNT TNNANTNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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