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YEplac195-SFK1 delta C

Yeast gene transduction vector of S. cerevisiae Sfk1p (SFK1) mutant lacking its C-terminal cytoplasmic region.

Catalog number RDB16715
Resource name YEplac195-SFK1 delta C
Alternative name pKT2194
Clone info. Yeast gene transduction vector of S. cerevisiae Sfk1p (SFK1) mutant that lacks the C-terminal cytoplasmic region (64 amino acids). Please refer to Fig.2B of Mioka, T. et al. Mol. Biol. Cell., 29 (10): 1203-1218, 2018 for the deletion.
Vector backbone YEplac195 (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), URA3 (Yeast)
Gene/insert name S. cerevisiae SFK1 genomic
Depositor|Developer Tanaka, Kazuma | Mioka, Tetsuo |
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External Database
S. cerevisiae SFK1
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mioka, T., Mol. Biol. Cell, 29(10), 1203-1218, 2018).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mioka, T., Mol. Biol. Cell, 29(10), 1203-1218, 2018)。
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          RDB16715 YEplac195-SFK1 delta C Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The YEplac195-SFK1 delta C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16715).

          Reference section:

          Mioka, T., Fujimura-Kamada, K., Mizugaki, N., Kishimoto, T., Sano, T., Nunome, H., Williams, D.E., Andersen, R.J., Tanaka, K., Phospholipid flippases and Sfk1p, a novel regulator of phospholipid asymmetry, contribute to low permeability of the plasma membrane. Mol. Biol. Cell 29(10): 1203-1218 (2018). PMID 29540528. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Mioka, T., Phospholipid flippases and Sfk1p, a novel regulator of phospholipid asymmetry, contribute to low permeability of the plasma membrane. Mol. Biol. Cell 29(10): 1203-1218 (2018). PMID 29540528. [link to RRC of NBRP]

          2023.08.17

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