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YEplac195-SFK1 delta C

Yeast gene transduction vector of S. cerevisiae Sfk1p (SFK1) mutant lacking its C-terminal cytoplasmic region.

Catalog number RDB16715
Resource name YEplac195-SFK1 delta C
Alternative name pKT2194
Clone info. Yeast gene transduction vector of S. cerevisiae Sfk1p (SFK1) mutant that lacks the C-terminal cytoplasmic region (64 amino acids). Please refer to Fig.2B of Mioka, T. et al. Mol. Biol. Cell., 29 (10): 1203-1218, 2018 for the deletion.
Vector backbone YEplac195 (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), URA3 (Yeast)
Gene/insert name S. cerevisiae SFK1 genomic
Depositor Tanaka, Kazuma | Mioka, Tetsuo |
 
Other clones in our bank S. cerevisiae SFK1 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mioka, T., Mol. Biol. Cell, 29(10), 1203-1218, 2018).
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RDB16715 YEplac195-SFK1 delta C Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Mioka, T., Phospholipid flippases and Sfk1p, a novel regulator of phospholipid asymmetry, contribute to low permeability of the plasma membrane. Mol. Biol. Cell 29(10): 1203-1218 (2018). PMID 29540528.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Mioka, T., Phospholipid flippases and Sfk1p, a novel regulator of phospholipid asymmetry, contribute to low permeability of the plasma membrane. Mol. Biol. Cell 29(10): 1203-1218 (2018). PMID 29540528.

2019.07.17

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