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pT2/shp53/shNf1/CMV-PDGFA-IRES-DsRed (#RDB16063)

The Sleeping Beauty (SB) transposon system clone. Expression vector of human PDGFA and shRNAs against mouse shNf1/shp53.

Alternative name PNP vector
Clone info. The Sleeping Beauty (SB) transposon system clone. Expression vector of human platelet-derived growth factor subunit A (PDGFA) and shRNAs against mouse Nf1 and Trp53 (shNf1/shp53). DsRed is expressed as a marker. Please refer to Fig.1A of Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018.
Comment Expression was confirmed by the depositor with immunohistochemistry. shRNA for Nf1 (5'-GGACACAATGAGATTAGAT-3') and Trp53 (5'-GTACATGTGTAATAGCTCC-3').
Vector backbone pT2/Onc2 (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r
Gene/insert name Human PDGFA cDNA mouse Nf1 cDNA mouse Trp53 cDNA
Depositor|Developer Koso, Hideto |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018).
Remarks This clone is provided directly from Dr. Hideto Koso of the Institute of Medical Science, Tokyo University.
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必要書類
提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018)。
備考 本クローンは、東京大学医科学研究所の高祖秀登先生から直接提供いたします。

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RDB16063 pT2/shp53/shNf1/CMV-PDGFA-IRES-DsRed DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pT2/shp53/shNf1/CMV-PDGFA-IRES-DsRed was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16063).

Reference section:

Sumiyoshi, K., Koso, H., Watanabe, S., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16063_A8Idp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB16063_A8Ida.seq check
>D05854A4-2_A8Id_CMV-Forward_B02_05_ABI24.ab1
    1 CTTTAAACTC CGGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGA
  121 GCTCAAGCTT CGAATTCACT AGTGATTGCG ATGAGGACCT TGGCTTGCCT GCTGCTCCTC
  181 GGCTGCGGAT ACCTCGCCCA TGTTCTGGCC GAGGAAGCCG AGATCCCCCG CGAGGTGATC
  241 GAGAGGCTGG CCCGCAGTCA GATCCACAGC ATCCGGGACC TCCAGCGACT CCTGGAGATA
  301 GACTCCGTAG GGAGTGAGGA TTCTTTGGAC ACCAGCCTGA GAGCTCACGG GGTCCACGCC
  361 ACTAAGCATG TGCCCGAGAA GCGGCCCCTG CCCATTCGGA GGAAGAGAAG CATCGAGGAA
  421 GCTGTCCCCG CTGTCTGCAA GACCAGGACG GTCATTTACG AGATTCCTCG GAGTCAGGTC
  481 GACCCCACGT CCGCCAACTT CCTGATCTGG CCCCCGTGCG TGGAGGTGAA ACGCTGCACC
  541 GGCTGCTGCA ACACGAGCAG TGTCAAGTGC CAGCCCTCCC GCGTCCACCA CCGCAGCGTC
  601 AAGGTGGCCA AGGTGGAATA CGTCAGGAAG AAGCCAAAAT TAAAAGAAGT CCAGGTGAGG
  661 TTAGAGGAGC ATTTGGAGTG CGCCTGCGCG ACCACAAGCC TGAATCCGGA TTATCGGGAA
  721 GAGGACACGG GAAGGCCTAG GGAGTCAGGT AAAAAACGGA AAAGAAAAAG GTTAAAACCC
  781 ACCTAAAGCA GCCAACCAGA TAATCGAATT CTGCAGTCGA CGGTACCGCG GGGCCCGGGG
  841 ATCCGCCCCT CTCCCTCCCC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAAATAA
  901 GGCCGGTGTG CGTTTGTCTA TATGTTAATT TTCCACCATA TTGCCGTCTT TTGGCAAATG
  961 TTAAGGGCCC GGAAAACCTG GACCCTGTCT TCTTTGAACG AAGCATTTCC TAGGGGGATC
1021 TTTCCCTCTC CGCTAAAGGA ATGCCAGGTC CTGATGAAAT GTCGTTGAAG GAAGGCA
//
Primer: IRES-M_R
Sequence file: RDB16063_A8Idb.seq check
>D05854A4-2_A8Id_IRES-M_R_C02_08_ABI24.ab1
    1 TTCTTCAGAG GACTGCTTCC TTCACGACAT TCAACAGACC TTGCATTCCT TTGGCGAGAG
   61 GGGAAAGACC CCTAGGAATG CTCGTCAAGA AGACAGGGCC AGGTTTCCGG GCCCTCACAT
  121 TGCCAAAAGA CGGCAATATG GTGGAAAATA ACATATAGAC AAACGCACAC CGGCCTTATT
  181 CCAAGCGGCT TCGGCCAGTA ACGTTAGGGG GGGGGGAGGG AGAGGGGCGG ATCCCGGGCC
  241 CGCGGTACCG TCGACTGCAG AATTCGATTA TCTGGTTGGC TGCTTTAGGT GGGTTTTAAC
  301 CTTTTTCTTT TCCGTTTTTT ACCTGACTCC CTAGGCCTTC CCGTGTCCTC TTCCCGATAA
  361 TCCGGATTCA GGCTTGTGGT CGCGCAGGCG CACTCCAAAT GCTCCTCTAA CCTCACCTGG
  421 ACTTCTTTTA ATTTTGGCTT CTTCCTGACG TATTCCACCT TGGCCACCTT GACGCTGCGG
  481 TGGTGGACGC GGGAGGGCTG GCACTTGACA CTGCTCGTGT TGCAGCAGCC GGTGCAGCGT
  541 TTCACCTCCA CGCACGGGGG CCAGATCAGG AAGTTGGCGG ACGTGGGGTC GACCTGACTC
  601 CGAGGAATCT CGTAAATGAC CGTCCTGGTC TTGCAGACAG CGGGGACAGC TTCCTCGATG
  661 CTTCTCTTCC TCCGAATGGG CAGGGGCCGC TTCTCGGGCA CATGCTTAGT GGCGTGGACC
  721 CCGTGAGCTC TCAGGCTGGT GTCCAAAGAA TCCTCACTCC CTACGGAGTC TATCTCCAGG
  781 AGTCGCTGGA GGTCCCGGAT GCTGTGGATC TGACTGCGGG CCAGCCTCTC GATCACCTCG
  841 CGGGGGGATC TCGGCTTCCT CGGCCAGAAC ATGGGCGAGG TATCCGCAGC CGAGGAGCAG
  901 CAGGCAAGCC AAGGTCCTCA TCGCAAATCA CTAGTGAATT CGAAGCTTGA GCTCGAGATC
  961 TGAGTCCGGG TAGCGCTAGC GGGATCTGAC GGTTTCACTA AACCAGCTCT GCTTATATAG
 1021 AACCTCCCAC CCGGTACACG GCCTAACCCG GCCAATTTTG GCCGTCCATG GGGCCGGAAG
1081 TGACTACCGC ACCATTTTGG AAAGGTTTCG C
//
Primer: M13_-40
Sequence file: RDB16063_A8Idc.seq check
>D05854A4-2_A8Id_M13_-40_A02_02_ABI24.ab1
    1 CGGCCAGTGA CGGCGCGTTA ATACGACTCA CTATAGGGCG AATTGGGAGC TCGGATCCCT
   61 ATACAGTTGA AGTCGGAAGT TTACATACAC TTAAGTTGGA GTCATTAAAA CTCGTTTTTC
  121 AACTACTCCA CAAATTTCTT GTTAACAAAC AATAGTTTTG GCAAGTCAGT TAGGACATCT
  181 ACTTTGTGCA TGACACAAGT CATTTTTCCA ACAATTGTTT ACAGACAGAT TATTTCACTT
  241 ATAATTCACT GTATCACAAT TCCAGTGGGT CAGAAGTTTA CATACACTAA GTTGACTGTG
  301 CCTTTAAACA GCTTGGAAAA TTCCAGAAAA TGATGTCATG GCTTTAGAAG CTTAAAAAGT
  361 ACATGTGTAA TAGCTCCTCT CTTGAAGGAG CTATTACACA TGTACGGGGA TCTGTGGTCT
  421 CATACAGAAC TTATAAGATT CCCAAATCCA AAGACATTTC ACGTTTATGG TGATTTCCCA
  481 GAACACATAG CGACATGCAA ATATTGCAGG GCGCCACTCC CCTGTCCCTC ACAGCCATCT
  541 TCCTGCCAGG GCGCACGCGC GCTGGGTGTT CCCGCCTAGT GACACTGGGC CCGCGATTCC
  601 TTGGAGCGGG TTGATGACGT CAGCGTTCGA ATTAGCTTGA TAGCTTAAAA AGGACACAAT
  661 GAGATTAGAT TCTCTTGAAA TCTAATCTCA TTGTGTCCGG GGATCTGTGG TCTCATACAG
  721 AACTTATAAG ATTCCCAAAT CCAAAGACAT TTCACGTTTA TGGTGATTTC CCAGAACACA
  781 TAGCGACATG CAAATATTGC AGGGCGCCAC TCCCCTGTCC CTCACAGCCA TCTTCCTGCC
  841 AGGGCGCACG CGCGCTGGGT GTTCCCCGCC TAGTGACACT GGGCCCGCGA TTCCTTGGAG
  901 CGGGTTTGAT GACGTCAGCG TTCGAATTAT CGGAATTCCG AAAGTTCCTA TTCTCTAGAA
  961 AGTATAAGGA AACTTCAGGT CCTGGAAGAG GAGTTTTACG TCCCAGGAAT ATCCTTAGTT
 1021 TATAATAGTA ATCAAATTAA CCGGGGTCCA TTAGTTTCAA TAGGCCGATA TTATGGGAGT
1081 TCGCGGTTAC AATAACCTTT AACGGGGT
//
Primer: mRFP-inner1
Sequence file: RDB16063_A8Idd.seq check
>D05854A4-2_A8Id_mRFP1-inner1_D02_11_ABI24.ab1
    1 GCAAGCGGGT GGGGAGTGAC CAGGACTCCT CCCTGCAGGA CGGCTCCTTC ATCTACAAGG
   61 TGAAGTTCAT CGGCGTGAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACTATGG
  121 GCTGGGAGGC CTCCACCGAG CGCCTGTACC CCCGCGACGG CGTGCTGAAG GGCGAGATCC
  181 ACAAGGCCCT GAAGCTGAAG GACGGCGGCC ACTACCTGGT GGAGTTCAAG TCTATCTATA
  241 TGGCCAAGAA GCCCGTGCAG CTGCCCGGCT ACTACTACGT GGACTCCAAG CTGGACATCA
  301 CCTCCCACAA CGAGGACTAC ACCATCGTGG AGCAGTACGA GCGCGCCGAG GGCCGCCACC
  361 ACCTGTTCCT GTAGCGGCCG CGATCAATTC TCTAGAGCTC GCTGATCAGC CTCGACTGTG
  421 CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA
  481 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT
  541 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA
  601 GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC
  661 AGCTGGGGCT CGACTAGAGC TTGCGGAAAA GCTAATTCCT ATCACAAACT AGGGGATCTA
  721 GCTTGTGGAA GGCTACTCGA AATGTTTGAC CCAAGTTAAA CAATTTAAAG GCAATGCTAC
  781 CAAATACTAA TTGAGTGTAT GTAAACTTCT GACCCACTGG GAATGTGATG AAAGAAATAA
  841 AAGCTGAAAT GAATCATTCT CTCTACTATT ATTCTGATAT TTCACATTCT TAAAATAAAG
  901 TGGTGATCCT AACTGACCTA AGACAGGGAA ATTTTTACTA GGATTAAATG TCAGGAATTG
  961 TGAAAAAAGT GAGTTTAAAT GTATTTGGCT AAGTGTATGT AAACTTCCGA CTTCACTGTA
 1021 TAGGAATCCT CTAGCTAGAG TCGAACCTTC GAAGGGGGAG CCGGTAACCC CAAGCCATTT
1081 TTGAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sumiyoshi, K., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [link to RRC of NBRP]