Please review the QC test results indicated by icon below as well as clone information before placing your order.

pT2/shp53/shNf1/CMV-PDGFA-IRES-DsRed

The Sleeping Beauty (SB) transposon system clone. Expression vector of human PDGFA and shRNAs against mouse shNf1/shp53.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018).
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link]
Remarks	This clone is provided directly from Dr. Hideto Koso of the Institute of Medical Science, Tokyo University.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018)。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	本クローンは、東京大学医科学研究所の高祖秀登先生から直接提供いたします。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16063_A8Idp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB16063_A8Ida.seq
>D05854A4-2_A8Id_CMV-Forward_B02_05_ABI24.ab1     1 CTTTAAACTC CGGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGA   121 GCTCAAGCTT CGAATTCACT AGTGATTGCG ATGAGGACCT TGGCTTGCCT GCTGCTCCTC   181 GGCTGCGGAT ACCTCGCCCA TGTTCTGGCC GAGGAAGCCG AGATCCCCCG CGAGGTGATC   241 GAGAGGCTGG CCCGCAGTCA GATCCACAGC ATCCGGGACC TCCAGCGACT CCTGGAGATA   301 GACTCCGTAG GGAGTGAGGA TTCTTTGGAC ACCAGCCTGA GAGCTCACGG GGTCCACGCC   361 ACTAAGCATG TGCCCGAGAA GCGGCCCCTG CCCATTCGGA GGAAGAGAAG CATCGAGGAA   421 GCTGTCCCCG CTGTCTGCAA GACCAGGACG GTCATTTACG AGATTCCTCG GAGTCAGGTC   481 GACCCCACGT CCGCCAACTT CCTGATCTGG CCCCCGTGCG TGGAGGTGAA ACGCTGCACC   541 GGCTGCTGCA ACACGAGCAG TGTCAAGTGC CAGCCCTCCC GCGTCCACCA CCGCAGCGTC   601 AAGGTGGCCA AGGTGGAATA CGTCAGGAAG AAGCCAAAAT TAAAAGAAGT CCAGGTGAGG   661 TTAGAGGAGC ATTTGGAGTG CGCCTGCGCG ACCACAAGCC TGAATCCGGA TTATCGGGAA   721 GAGGACACGG GAAGGCCTAG GGAGTCAGGT AAAAAACGGA AAAGAAAAAG GTTAAAACCC   781 ACCTAAAGCA GCCAACCAGA TAATCGAATT CTGCAGTCGA CGGTACCGCG GGGCCCGGGG   841 ATCCGCCCCT CTCCCTCCCC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAAATAA   901 GGCCGGTGTG CGTTTGTCTA TATGTTAATT TTCCACCATA TTGCCGTCTT TTGGCAAATG   961 TTAAGGGCCC GGAAAACCTG GACCCTGTCT TCTTTGAACG AAGCATTTCC TAGGGGGATC 1021 TTTCCCTCTC CGCTAAAGGA ATGCCAGGTC CTGATGAAAT GTCGTTGAAG GAAGGCA //
Primer: IRES-M_R Sequence file: RDB16063_A8Idb.seq
>D05854A4-2_A8Id_IRES-M_R_C02_08_ABI24.ab1     1 TTCTTCAGAG GACTGCTTCC TTCACGACAT TCAACAGACC TTGCATTCCT TTGGCGAGAG    61 GGGAAAGACC CCTAGGAATG CTCGTCAAGA AGACAGGGCC AGGTTTCCGG GCCCTCACAT   121 TGCCAAAAGA CGGCAATATG GTGGAAAATA ACATATAGAC AAACGCACAC CGGCCTTATT   181 CCAAGCGGCT TCGGCCAGTA ACGTTAGGGG GGGGGGAGGG AGAGGGGCGG ATCCCGGGCC   241 CGCGGTACCG TCGACTGCAG AATTCGATTA TCTGGTTGGC TGCTTTAGGT GGGTTTTAAC   301 CTTTTTCTTT TCCGTTTTTT ACCTGACTCC CTAGGCCTTC CCGTGTCCTC TTCCCGATAA   361 TCCGGATTCA GGCTTGTGGT CGCGCAGGCG CACTCCAAAT GCTCCTCTAA CCTCACCTGG   421 ACTTCTTTTA ATTTTGGCTT CTTCCTGACG TATTCCACCT TGGCCACCTT GACGCTGCGG   481 TGGTGGACGC GGGAGGGCTG GCACTTGACA CTGCTCGTGT TGCAGCAGCC GGTGCAGCGT   541 TTCACCTCCA CGCACGGGGG CCAGATCAGG AAGTTGGCGG ACGTGGGGTC GACCTGACTC   601 CGAGGAATCT CGTAAATGAC CGTCCTGGTC TTGCAGACAG CGGGGACAGC TTCCTCGATG   661 CTTCTCTTCC TCCGAATGGG CAGGGGCCGC TTCTCGGGCA CATGCTTAGT GGCGTGGACC   721 CCGTGAGCTC TCAGGCTGGT GTCCAAAGAA TCCTCACTCC CTACGGAGTC TATCTCCAGG   781 AGTCGCTGGA GGTCCCGGAT GCTGTGGATC TGACTGCGGG CCAGCCTCTC GATCACCTCG   841 CGGGGGGATC TCGGCTTCCT CGGCCAGAAC ATGGGCGAGG TATCCGCAGC CGAGGAGCAG   901 CAGGCAAGCC AAGGTCCTCA TCGCAAATCA CTAGTGAATT CGAAGCTTGA GCTCGAGATC   961 TGAGTCCGGG TAGCGCTAGC GGGATCTGAC GGTTTCACTA AACCAGCTCT GCTTATATAG  1021 AACCTCCCAC CCGGTACACG GCCTAACCCG GCCAATTTTG GCCGTCCATG GGGCCGGAAG 1081 TGACTACCGC ACCATTTTGG AAAGGTTTCG C //
Primer: M13_-40 Sequence file: RDB16063_A8Idc.seq
>D05854A4-2_A8Id_M13_-40_A02_02_ABI24.ab1     1 CGGCCAGTGA CGGCGCGTTA ATACGACTCA CTATAGGGCG AATTGGGAGC TCGGATCCCT    61 ATACAGTTGA AGTCGGAAGT TTACATACAC TTAAGTTGGA GTCATTAAAA CTCGTTTTTC   121 AACTACTCCA CAAATTTCTT GTTAACAAAC AATAGTTTTG GCAAGTCAGT TAGGACATCT   181 ACTTTGTGCA TGACACAAGT CATTTTTCCA ACAATTGTTT ACAGACAGAT TATTTCACTT   241 ATAATTCACT GTATCACAAT TCCAGTGGGT CAGAAGTTTA CATACACTAA GTTGACTGTG   301 CCTTTAAACA GCTTGGAAAA TTCCAGAAAA TGATGTCATG GCTTTAGAAG CTTAAAAAGT   361 ACATGTGTAA TAGCTCCTCT CTTGAAGGAG CTATTACACA TGTACGGGGA TCTGTGGTCT   421 CATACAGAAC TTATAAGATT CCCAAATCCA AAGACATTTC ACGTTTATGG TGATTTCCCA   481 GAACACATAG CGACATGCAA ATATTGCAGG GCGCCACTCC CCTGTCCCTC ACAGCCATCT   541 TCCTGCCAGG GCGCACGCGC GCTGGGTGTT CCCGCCTAGT GACACTGGGC CCGCGATTCC   601 TTGGAGCGGG TTGATGACGT CAGCGTTCGA ATTAGCTTGA TAGCTTAAAA AGGACACAAT   661 GAGATTAGAT TCTCTTGAAA TCTAATCTCA TTGTGTCCGG GGATCTGTGG TCTCATACAG   721 AACTTATAAG ATTCCCAAAT CCAAAGACAT TTCACGTTTA TGGTGATTTC CCAGAACACA   781 TAGCGACATG CAAATATTGC AGGGCGCCAC TCCCCTGTCC CTCACAGCCA TCTTCCTGCC   841 AGGGCGCACG CGCGCTGGGT GTTCCCCGCC TAGTGACACT GGGCCCGCGA TTCCTTGGAG   901 CGGGTTTGAT GACGTCAGCG TTCGAATTAT CGGAATTCCG AAAGTTCCTA TTCTCTAGAA   961 AGTATAAGGA AACTTCAGGT CCTGGAAGAG GAGTTTTACG TCCCAGGAAT ATCCTTAGTT  1021 TATAATAGTA ATCAAATTAA CCGGGGTCCA TTAGTTTCAA TAGGCCGATA TTATGGGAGT 1081 TCGCGGTTAC AATAACCTTT AACGGGGT //
Primer: mRFP-inner1 Sequence file: RDB16063_A8Idd.seq
>D05854A4-2_A8Id_mRFP1-inner1_D02_11_ABI24.ab1     1 GCAAGCGGGT GGGGAGTGAC CAGGACTCCT CCCTGCAGGA CGGCTCCTTC ATCTACAAGG    61 TGAAGTTCAT CGGCGTGAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACTATGG   121 GCTGGGAGGC CTCCACCGAG CGCCTGTACC CCCGCGACGG CGTGCTGAAG GGCGAGATCC   181 ACAAGGCCCT GAAGCTGAAG GACGGCGGCC ACTACCTGGT GGAGTTCAAG TCTATCTATA   241 TGGCCAAGAA GCCCGTGCAG CTGCCCGGCT ACTACTACGT GGACTCCAAG CTGGACATCA   301 CCTCCCACAA CGAGGACTAC ACCATCGTGG AGCAGTACGA GCGCGCCGAG GGCCGCCACC   361 ACCTGTTCCT GTAGCGGCCG CGATCAATTC TCTAGAGCTC GCTGATCAGC CTCGACTGTG   421 CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA   481 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT   541 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA   601 GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC   661 AGCTGGGGCT CGACTAGAGC TTGCGGAAAA GCTAATTCCT ATCACAAACT AGGGGATCTA   721 GCTTGTGGAA GGCTACTCGA AATGTTTGAC CCAAGTTAAA CAATTTAAAG GCAATGCTAC   781 CAAATACTAA TTGAGTGTAT GTAAACTTCT GACCCACTGG GAATGTGATG AAAGAAATAA   841 AAGCTGAAAT GAATCATTCT CTCTACTATT ATTCTGATAT TTCACATTCT TAAAATAAAG   901 TGGTGATCCT AACTGACCTA AGACAGGGAA ATTTTTACTA GGATTAAATG TCAGGAATTG   961 TGAAAAAAGT GAGTTTAAAT GTATTTGGCT AAGTGTATGT AAACTTCCGA CTTCACTGTA  1021 TAGGAATCCT CTAGCTAGAG TCGAACCTTC GAAGGGGGAG CCGGTAACCC CAAGCCATTT 1081 TTGAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.12.13

GNP_filter3_RDBDEP_html_231213.pl