Please review the QC test results indicated by icon below as well as clone information before placing your order.

pT2/shp53/shNf1/SV40-GFP

The Sleeping Beauty (SB) transposon system clone. Expression vector of shRNAs against mouse shNf1/shp53.

Distribution information

Ordering	This clone is provided directly from Dr. Hideto Koso of the Institute of Medical Science, Tokyo University.

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References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16062_A8Idp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB16062_A8Ida.seq
>D05854A3-3_A8Id_M13_-40_E01_13_ABI24.ab1     1 AACCAGGTGA CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCG GATCCCTATA    61 CAGTTGAAGT CGGAAGTTTA CATACACTTA AGTTGGAGTC ATTAAAACTC GTTTTTCAAC   121 TACTCCACAA ATTTCTTGTT AACAAACAAT AGTTTTGGCA AGTCAGTTAG GACATCTACT   181 TTGTGCATGA CACAAGTCAT TTTTCCAACA ATTGTTTACA GACAGATTAT TTCACTTATA   241 ATTCACTGTA TCACAATTCC AGTGGGTCAG AAGTTTACAT ACACTAAGTT GACTGTGCCT   301 TTAAACAGCT TGGAAAATTC CAGAAAATGA TGTCATGGCT TTAGAAGCTT AAAAAGTACA   361 TGTGTAATAG CTCCTCTCTT GAAGGAGCTA TTACACATGT ACGGGGATCT GTGGTCTCAT   421 ACAGAACTTA TAAGATTCCC AAATCCAAAG ACATTTCACG TTTATGGTGA TTTCCCAGAA   481 CACATAGCGA CATGCAAATA TTGCAGGGCG CCACTCCCCT GTCCCTCACA GCCATCTTCC   541 TGCCAGGGCG CACGCGCGCT GGGTGTTCCC GCCTAGTGAC ACTGGGCCCG CGATTCCTTG   601 GAGCGGGTTG ATGACGTCAG CGTTCGAATT AGCTTGATAG CTTAAAAAGG ACACAATGAG   661 ATTAGATTCT CTTGAAATCT AATCTCATTG TGTCCGGGGA TCTGTGGTCT CATACAGAAC   721 TTATAAGATT CCCAAATCCA AAGACATTTC ACGTTTATGG TGATTTCCCA GAACACATAG   781 CGACATGCAA ATATTGCAGG GCGCCACTCC CCTGTCCCTC ACAGCCATCT TCCTGCCAGG   841 GCGCACGCGC GCTGGGTGTT CCCGCCTAGT GACACTGGGC CCGCGATTCC TTGGGAGCGG   901 GTTGATGACG TCAGCGTTCG AAATTATCGA AATTCCGAAG TTCCTATTTC TCTAGAAAGT   961 ATAGGAAACT TCAGTCTGAA GAGAGTTTAC GTCCAGATAT TCTTCAAATT TCGCCAAGCA  1021 CAGTGGGTCG ACCCTGTTGA ATGGTGTGTC AGTTAGGTGT GAAGTCCCCA GGCTCCCCAG 1081 GCAAGGTCCA GTAG //
Primer: SV40pro_R2 Sequence file: RDB16062_A8Idb.seq
>D05854A3-3_A8Id_SV40pro_R2_F01_16_ABI24.ab1     1 TATCAGCATT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG    61 ACACACATTC CACAGGGTCG ACCACTGTGC TGGCGAATTG AAGATATCTG GACGTAAACT   121 CCTCTTCAGA CCTGAAGTTC CTATACTTTC TAGAGAATAG GAACTTCGGA ATTCGATAAT   181 TCGAACGCTG ACGTCATCAA CCCGCTCCAA GGAATCGCGG GCCCAGTGTC ACTAGGCGGG   241 AACACCCAGC GCGCGTGCGC CCTGGCAGGA AGATGGCTGT GAGGGACAGG GGAGTGGCGC   301 CCTGCAATAT TTGCATGTCG CTATGTGTTC TGGGAAATCA CCATAAACGT GAAATGTCTT   361 TGGATTTGGG AATCTTATAA GTTCTGTATG AGACCACAGA TCCCCGGACA CAATGAGATT   421 AGATTTCAAG AGAATCTAAT CTCATTGTGT CCTTTTTAAG CTATCAAGCT AATTCGAACG   481 CTGACGTCAT CAACCCGCTC CAAGGAATCG CGGGCCCAGT GTCACTAGGC GGGAACACCC   541 AGCGCGCGTG CGCCCTGGCA GGAAGATGGC TGTGAGGGAC AGGGGAGTGG CGCCCTGCAA   601 TATTTGCATG TCGCTATGTG TTCTGGGAAA TCACCATAAA CGTGAAATGT CTTTGGATTT   661 GGGAATCTTA TAAGTTCTGT ATGAGACCAC AGATCCCCGT ACATGTGTAA TAGCTCCTTC   721 AAGAGAGGAG CTATTACACA TGTACTTTTT AAGCTTCTAA AGCCATGACA TCATTTTCTG   781 GAATTTTCCA AGCTGTTTAA AGGCACAGTC AACTTAGTGT ATGTAAACTT CTGACCCACT   841 GGAATTGTGA TACAGTGAAT TATAAGTGAA ATAATCTGTC TGTAAACAAT TGTTGGAAAA   901 AATGACTTGT GTCATGCACA AAGTAGATGT CCTAACTGAC TTGCCCAAAA CTATTTGTTT   961 GTTAACAAGA AATTTGTGGG AGTAGTTTGA AAAACGAGTT TTTATGACTC GACTTAAGTG  1021 TATGTAAACT TCCGACTCAC TGGTATAGGA TCCGAGCTCG AATCGCCTTA TAGTGAAGTT 1081 CGGTTATTTA CGGACG //
Primer: SV40pro_F Sequence file: RDB16062_A8Idc.seq
>D05854A3-3_A8Id_SV40pro_F_G01_19_ABI24.ab1     1 CATCGGGCTG GCAATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA    61 AAGCTCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG   121 CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCTG GCGAGGGCGA GGGCGATGCC   181 ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG   241 CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC   301 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC   361 ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC   421 ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG   481 GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG   541 AAGAACGGCA TCAAGGCGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG   601 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC   661 AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC   721 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC   781 AAGTAATGAG CGGCCGCGAT CAATTCTCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT   841 CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCCGTGC CTTCCTTGAC CCTGGAAAGG   901 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT TGTCTGAGTA   961 GGTGTCATTC TATTCTGGGG GGGGTGGGGT GGGGGCAGAC AGCCAAGGGG GAGGATTTGG 1021 AAGACCATAG CCAGCATGCT GGGATGCCGG TGGGCTCTAT GGCTTCCTGA G //
Primer: EGFP-C2 Sequence file: RDB16062_A8Idd.seq
>D05854A3-3_A8Id_EGFP-C2_H01_22_ABI24.ab1     1 GACCAAAGGA AAAAGGCGCG GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA    61 TCACTGGTCG GCATGGACGA GCTGTACAAG TAATGAGCGG CCGCGATCAA TTCTCTAGAG   121 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC   181 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG   241 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG   301 ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA   361 TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCGACTAG AGCTTGCGGA AAAGCTAATT   421 CCTATCACAA ACTAGGGGAT CTAGCTTGTG GAAGGCTACT CGAAATGTTT GACCCAAGTT   481 AAACAATTTA AAGGCAATGC TACCAAATAC TAATTGAGTG TATGTAAAAC TTCTGACCCA   541 CTGGGAATGT GATGAAAGAA ATAAAAGCTG AAAATGAATC ATTCTCTCTA CTATTATTCT   601 GATATTTCAC ATTCTTAAAA TAAAGTGGTG ATCCTAAACT GACCTAAAGA CAGGGAATTT   661 TTACTAGGAT TAAATGTCAG AATTGTGAAA AAGTGAGTTT AAATGTATTT GGCTAAGGTG   721 TATGTAAACT TCCGAACTTC AACTGTATAG GGATCCTCTA GCTAGAGTCG ACCTCGAAGG   781 GGGGGGCCCG GGTACCCCAG CCTTTTGTTC CCTTTTAGTG AAGGGTTAAA TTGGCGCGCT   841 TTGGGCGTAA ATCATGGGTC AATAACCTGG TTTCCTGTGT GAAAATTGTA ATCCGCCTCA   901 CAAATTCCCA CCAACAAACA ATACCGAAGC CACGGAAAGC CATAAAAGTT GTAAAAGCCT   961 TGGGGTTGCC CTTAAATGAA GGTGGAGCCT AACCTCACCA ATTAAAATTG GCCGTTGCCG  1021 CTCCACTTGC CCCGCCTTTT TCCAAGATTC CGGGAAAAAC CCTTGATCCG TTGGCCAAGC  1081 CCTGCCCAAT AAAATGGAAT TCCGGCCAAA ACGTCGCCGG GAGAAAAGAG CCGGTTATAG 1141 GCCGATTATA TTGGGGCGAC G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl