Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pT2/shp53/shNf1/SV40-GFP

The Sleeping Beauty (SB) transposon system clone. Expression vector of shRNAs against mouse shNf1/shp53.

Catalog number RDB16062
Resource name pT2/shp53/shNf1/SV40-GFP
Alternative name NP vector
Clone info. The Sleeping Beauty (SB) transposon system clone. Expression vector of shRNAs against mouse Nf1 and Trp53 (shNf1/shp53). GFP is expressed as a marker. Please refer to Fig.1A of Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018.
Comment Expression was confirmed by the depositor with immunohistochemistry. shRNA for Nf1 (5'-GGACACAATGAGATTAGAT-3') and Trp53 (5'-GTACATGTGTAATAGCTCC-3').
Vector backbone pT2/Onc2 (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Nf1 cDNA mouse Trp53 cDNA
Depositor|Developer Koso, Hideto |
Other clones in our bank

External Database
Mouse Nf1 mouse Trp53

        Reference sequence
          

        Distribution information

        Ordering This clone is provided directly from Dr. Hideto Koso of the Institute of Medical Science, Tokyo University.
        提供案内 (日本国内) [open/close]

        提供依頼 本クローンは、東京大学医科学研究所の高祖秀登先生から直接提供いたします。


        References and tips

        Electronic file

        Original reference

        original Sumiyoshi, K., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648.

        Further references such as user reports and related articles (go to bottom)

        Featured content


        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB16062_A8Idp1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: M13_-40
        Sequence file: RDB16062_A8Ida.seq check
        >D05854A3-3_A8Id_M13_-40_E01_13_ABI24.ab1
            1 AACCAGGTGA CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCG GATCCCTATA
           61 CAGTTGAAGT CGGAAGTTTA CATACACTTA AGTTGGAGTC ATTAAAACTC GTTTTTCAAC
          121 TACTCCACAA ATTTCTTGTT AACAAACAAT AGTTTTGGCA AGTCAGTTAG GACATCTACT
          181 TTGTGCATGA CACAAGTCAT TTTTCCAACA ATTGTTTACA GACAGATTAT TTCACTTATA
          241 ATTCACTGTA TCACAATTCC AGTGGGTCAG AAGTTTACAT ACACTAAGTT GACTGTGCCT
          301 TTAAACAGCT TGGAAAATTC CAGAAAATGA TGTCATGGCT TTAGAAGCTT AAAAAGTACA
          361 TGTGTAATAG CTCCTCTCTT GAAGGAGCTA TTACACATGT ACGGGGATCT GTGGTCTCAT
          421 ACAGAACTTA TAAGATTCCC AAATCCAAAG ACATTTCACG TTTATGGTGA TTTCCCAGAA
          481 CACATAGCGA CATGCAAATA TTGCAGGGCG CCACTCCCCT GTCCCTCACA GCCATCTTCC
          541 TGCCAGGGCG CACGCGCGCT GGGTGTTCCC GCCTAGTGAC ACTGGGCCCG CGATTCCTTG
          601 GAGCGGGTTG ATGACGTCAG CGTTCGAATT AGCTTGATAG CTTAAAAAGG ACACAATGAG
          661 ATTAGATTCT CTTGAAATCT AATCTCATTG TGTCCGGGGA TCTGTGGTCT CATACAGAAC
          721 TTATAAGATT CCCAAATCCA AAGACATTTC ACGTTTATGG TGATTTCCCA GAACACATAG
          781 CGACATGCAA ATATTGCAGG GCGCCACTCC CCTGTCCCTC ACAGCCATCT TCCTGCCAGG
          841 GCGCACGCGC GCTGGGTGTT CCCGCCTAGT GACACTGGGC CCGCGATTCC TTGGGAGCGG
          901 GTTGATGACG TCAGCGTTCG AAATTATCGA AATTCCGAAG TTCCTATTTC TCTAGAAAGT
          961 ATAGGAAACT TCAGTCTGAA GAGAGTTTAC GTCCAGATAT TCTTCAAATT TCGCCAAGCA
         1021 CAGTGGGTCG ACCCTGTTGA ATGGTGTGTC AGTTAGGTGT GAAGTCCCCA GGCTCCCCAG
        1081 GCAAGGTCCA GTAG
        //
        Primer: SV40pro_R2
        Sequence file: RDB16062_A8Idb.seq check
        >D05854A3-3_A8Id_SV40pro_R2_F01_16_ABI24.ab1
            1 TATCAGCATT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG
           61 ACACACATTC CACAGGGTCG ACCACTGTGC TGGCGAATTG AAGATATCTG GACGTAAACT
          121 CCTCTTCAGA CCTGAAGTTC CTATACTTTC TAGAGAATAG GAACTTCGGA ATTCGATAAT
          181 TCGAACGCTG ACGTCATCAA CCCGCTCCAA GGAATCGCGG GCCCAGTGTC ACTAGGCGGG
          241 AACACCCAGC GCGCGTGCGC CCTGGCAGGA AGATGGCTGT GAGGGACAGG GGAGTGGCGC
          301 CCTGCAATAT TTGCATGTCG CTATGTGTTC TGGGAAATCA CCATAAACGT GAAATGTCTT
          361 TGGATTTGGG AATCTTATAA GTTCTGTATG AGACCACAGA TCCCCGGACA CAATGAGATT
          421 AGATTTCAAG AGAATCTAAT CTCATTGTGT CCTTTTTAAG CTATCAAGCT AATTCGAACG
          481 CTGACGTCAT CAACCCGCTC CAAGGAATCG CGGGCCCAGT GTCACTAGGC GGGAACACCC
          541 AGCGCGCGTG CGCCCTGGCA GGAAGATGGC TGTGAGGGAC AGGGGAGTGG CGCCCTGCAA
          601 TATTTGCATG TCGCTATGTG TTCTGGGAAA TCACCATAAA CGTGAAATGT CTTTGGATTT
          661 GGGAATCTTA TAAGTTCTGT ATGAGACCAC AGATCCCCGT ACATGTGTAA TAGCTCCTTC
          721 AAGAGAGGAG CTATTACACA TGTACTTTTT AAGCTTCTAA AGCCATGACA TCATTTTCTG
          781 GAATTTTCCA AGCTGTTTAA AGGCACAGTC AACTTAGTGT ATGTAAACTT CTGACCCACT
          841 GGAATTGTGA TACAGTGAAT TATAAGTGAA ATAATCTGTC TGTAAACAAT TGTTGGAAAA
          901 AATGACTTGT GTCATGCACA AAGTAGATGT CCTAACTGAC TTGCCCAAAA CTATTTGTTT
          961 GTTAACAAGA AATTTGTGGG AGTAGTTTGA AAAACGAGTT TTTATGACTC GACTTAAGTG
         1021 TATGTAAACT TCCGACTCAC TGGTATAGGA TCCGAGCTCG AATCGCCTTA TAGTGAAGTT
        1081 CGGTTATTTA CGGACG
        //
        Primer: SV40pro_F
        Sequence file: RDB16062_A8Idc.seq check
        >D05854A3-3_A8Id_SV40pro_F_G01_19_ABI24.ab1
            1 CATCGGGCTG GCAATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
           61 AAGCTCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG
          121 CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCTG GCGAGGGCGA GGGCGATGCC
          181 ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG
          241 CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC
          301 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC
          361 ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC
          421 ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG
          481 GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG
          541 AAGAACGGCA TCAAGGCGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG
          601 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC
          661 AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC
          721 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC
          781 AAGTAATGAG CGGCCGCGAT CAATTCTCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT
          841 CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCCGTGC CTTCCTTGAC CCTGGAAAGG
          901 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT TGTCTGAGTA
          961 GGTGTCATTC TATTCTGGGG GGGGTGGGGT GGGGGCAGAC AGCCAAGGGG GAGGATTTGG
        1021 AAGACCATAG CCAGCATGCT GGGATGCCGG TGGGCTCTAT GGCTTCCTGA G
        //
        Primer: EGFP-C2
        Sequence file: RDB16062_A8Idd.seq check
        >D05854A3-3_A8Id_EGFP-C2_H01_22_ABI24.ab1
            1 GACCAAAGGA AAAAGGCGCG GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA
           61 TCACTGGTCG GCATGGACGA GCTGTACAAG TAATGAGCGG CCGCGATCAA TTCTCTAGAG
          121 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
          181 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG
          241 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG
          301 ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA
          361 TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCGACTAG AGCTTGCGGA AAAGCTAATT
          421 CCTATCACAA ACTAGGGGAT CTAGCTTGTG GAAGGCTACT CGAAATGTTT GACCCAAGTT
          481 AAACAATTTA AAGGCAATGC TACCAAATAC TAATTGAGTG TATGTAAAAC TTCTGACCCA
          541 CTGGGAATGT GATGAAAGAA ATAAAAGCTG AAAATGAATC ATTCTCTCTA CTATTATTCT
          601 GATATTTCAC ATTCTTAAAA TAAAGTGGTG ATCCTAAACT GACCTAAAGA CAGGGAATTT
          661 TTACTAGGAT TAAATGTCAG AATTGTGAAA AAGTGAGTTT AAATGTATTT GGCTAAGGTG
          721 TATGTAAACT TCCGAACTTC AACTGTATAG GGATCCTCTA GCTAGAGTCG ACCTCGAAGG
          781 GGGGGGCCCG GGTACCCCAG CCTTTTGTTC CCTTTTAGTG AAGGGTTAAA TTGGCGCGCT
          841 TTGGGCGTAA ATCATGGGTC AATAACCTGG TTTCCTGTGT GAAAATTGTA ATCCGCCTCA
          901 CAAATTCCCA CCAACAAACA ATACCGAAGC CACGGAAAGC CATAAAAGTT GTAAAAGCCT
          961 TGGGGTTGCC CTTAAATGAA GGTGGAGCCT AACCTCACCA ATTAAAATTG GCCGTTGCCG
         1021 CTCCACTTGC CCCGCCTTTT TCCAAGATTC CGGGAAAAAC CCTTGATCCG TTGGCCAAGC
         1081 CCTGCCCAAT AAAATGGAAT TCCGGCCAAA ACGTCGCCGG GAGAAAAGAG CCGGTTATAG
        1141 GCCGATTATA TTGGGGCGAC G
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sumiyoshi, K., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl