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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pT2/shp53/shNf1/SV40-GFP (#RDB16062)

The Sleeping Beauty (SB) transposon system clone. Expression vector of shRNAs against mouse shNf1/shp53.

Alternative name NP vector
Clone info. The Sleeping Beauty (SB) transposon system clone. Expression vector of shRNAs against mouse Nf1 and Trp53 (shNf1/shp53). GFP is expressed as a marker. Please refer to Fig.1A of Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018.
Comment Expression was confirmed by the depositor with immunohistochemistry. shRNA for Nf1 (5'-GGACACAATGAGATTAGAT-3') and Trp53 (5'-GTACATGTGTAATAGCTCC-3').
Vector backbone pT2/Onc2 (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Nf1 cDNA mouse Trp53 cDNA
Depositor|Developer Koso, Hideto |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018).
Remarks This clone is provided directly from Dr. Hideto Koso of the Institute of Medical Science, Tokyo University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018)。
備考 本クローンは、東京大学医科学研究所の高祖秀登先生から直接提供いたします。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16062 pT2/shp53/shNf1/SV40-GFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pT2/shp53/shNf1/SV40-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16062).

Reference section:

Sumiyoshi, K., Koso, H., Watanabe, S., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16062_A8Idp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB16062_A8Ida.seq check
>D05854A3-3_A8Id_M13_-40_E01_13_ABI24.ab1
    1 AACCAGGTGA CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCG GATCCCTATA
   61 CAGTTGAAGT CGGAAGTTTA CATACACTTA AGTTGGAGTC ATTAAAACTC GTTTTTCAAC
  121 TACTCCACAA ATTTCTTGTT AACAAACAAT AGTTTTGGCA AGTCAGTTAG GACATCTACT
  181 TTGTGCATGA CACAAGTCAT TTTTCCAACA ATTGTTTACA GACAGATTAT TTCACTTATA
  241 ATTCACTGTA TCACAATTCC AGTGGGTCAG AAGTTTACAT ACACTAAGTT GACTGTGCCT
  301 TTAAACAGCT TGGAAAATTC CAGAAAATGA TGTCATGGCT TTAGAAGCTT AAAAAGTACA
  361 TGTGTAATAG CTCCTCTCTT GAAGGAGCTA TTACACATGT ACGGGGATCT GTGGTCTCAT
  421 ACAGAACTTA TAAGATTCCC AAATCCAAAG ACATTTCACG TTTATGGTGA TTTCCCAGAA
  481 CACATAGCGA CATGCAAATA TTGCAGGGCG CCACTCCCCT GTCCCTCACA GCCATCTTCC
  541 TGCCAGGGCG CACGCGCGCT GGGTGTTCCC GCCTAGTGAC ACTGGGCCCG CGATTCCTTG
  601 GAGCGGGTTG ATGACGTCAG CGTTCGAATT AGCTTGATAG CTTAAAAAGG ACACAATGAG
  661 ATTAGATTCT CTTGAAATCT AATCTCATTG TGTCCGGGGA TCTGTGGTCT CATACAGAAC
  721 TTATAAGATT CCCAAATCCA AAGACATTTC ACGTTTATGG TGATTTCCCA GAACACATAG
  781 CGACATGCAA ATATTGCAGG GCGCCACTCC CCTGTCCCTC ACAGCCATCT TCCTGCCAGG
  841 GCGCACGCGC GCTGGGTGTT CCCGCCTAGT GACACTGGGC CCGCGATTCC TTGGGAGCGG
  901 GTTGATGACG TCAGCGTTCG AAATTATCGA AATTCCGAAG TTCCTATTTC TCTAGAAAGT
  961 ATAGGAAACT TCAGTCTGAA GAGAGTTTAC GTCCAGATAT TCTTCAAATT TCGCCAAGCA
 1021 CAGTGGGTCG ACCCTGTTGA ATGGTGTGTC AGTTAGGTGT GAAGTCCCCA GGCTCCCCAG
1081 GCAAGGTCCA GTAG
//
Primer: SV40pro_R2
Sequence file: RDB16062_A8Idb.seq check
>D05854A3-3_A8Id_SV40pro_R2_F01_16_ABI24.ab1
    1 TATCAGCATT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG
   61 ACACACATTC CACAGGGTCG ACCACTGTGC TGGCGAATTG AAGATATCTG GACGTAAACT
  121 CCTCTTCAGA CCTGAAGTTC CTATACTTTC TAGAGAATAG GAACTTCGGA ATTCGATAAT
  181 TCGAACGCTG ACGTCATCAA CCCGCTCCAA GGAATCGCGG GCCCAGTGTC ACTAGGCGGG
  241 AACACCCAGC GCGCGTGCGC CCTGGCAGGA AGATGGCTGT GAGGGACAGG GGAGTGGCGC
  301 CCTGCAATAT TTGCATGTCG CTATGTGTTC TGGGAAATCA CCATAAACGT GAAATGTCTT
  361 TGGATTTGGG AATCTTATAA GTTCTGTATG AGACCACAGA TCCCCGGACA CAATGAGATT
  421 AGATTTCAAG AGAATCTAAT CTCATTGTGT CCTTTTTAAG CTATCAAGCT AATTCGAACG
  481 CTGACGTCAT CAACCCGCTC CAAGGAATCG CGGGCCCAGT GTCACTAGGC GGGAACACCC
  541 AGCGCGCGTG CGCCCTGGCA GGAAGATGGC TGTGAGGGAC AGGGGAGTGG CGCCCTGCAA
  601 TATTTGCATG TCGCTATGTG TTCTGGGAAA TCACCATAAA CGTGAAATGT CTTTGGATTT
  661 GGGAATCTTA TAAGTTCTGT ATGAGACCAC AGATCCCCGT ACATGTGTAA TAGCTCCTTC
  721 AAGAGAGGAG CTATTACACA TGTACTTTTT AAGCTTCTAA AGCCATGACA TCATTTTCTG
  781 GAATTTTCCA AGCTGTTTAA AGGCACAGTC AACTTAGTGT ATGTAAACTT CTGACCCACT
  841 GGAATTGTGA TACAGTGAAT TATAAGTGAA ATAATCTGTC TGTAAACAAT TGTTGGAAAA
  901 AATGACTTGT GTCATGCACA AAGTAGATGT CCTAACTGAC TTGCCCAAAA CTATTTGTTT
  961 GTTAACAAGA AATTTGTGGG AGTAGTTTGA AAAACGAGTT TTTATGACTC GACTTAAGTG
 1021 TATGTAAACT TCCGACTCAC TGGTATAGGA TCCGAGCTCG AATCGCCTTA TAGTGAAGTT
1081 CGGTTATTTA CGGACG
//
Primer: SV40pro_F
Sequence file: RDB16062_A8Idc.seq check
>D05854A3-3_A8Id_SV40pro_F_G01_19_ABI24.ab1
    1 CATCGGGCTG GCAATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG
  121 CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCTG GCGAGGGCGA GGGCGATGCC
  181 ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG
  241 CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC
  301 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC
  361 ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC
  421 ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG
  481 GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG
  541 AAGAACGGCA TCAAGGCGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG
  601 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC
  661 AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC
  721 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC
  781 AAGTAATGAG CGGCCGCGAT CAATTCTCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT
  841 CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCCGTGC CTTCCTTGAC CCTGGAAAGG
  901 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT TGTCTGAGTA
  961 GGTGTCATTC TATTCTGGGG GGGGTGGGGT GGGGGCAGAC AGCCAAGGGG GAGGATTTGG
1021 AAGACCATAG CCAGCATGCT GGGATGCCGG TGGGCTCTAT GGCTTCCTGA G
//
Primer: EGFP-C2
Sequence file: RDB16062_A8Idd.seq check
>D05854A3-3_A8Id_EGFP-C2_H01_22_ABI24.ab1
    1 GACCAAAGGA AAAAGGCGCG GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA
   61 TCACTGGTCG GCATGGACGA GCTGTACAAG TAATGAGCGG CCGCGATCAA TTCTCTAGAG
  121 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
  181 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG
  241 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG
  301 ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA
  361 TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCGACTAG AGCTTGCGGA AAAGCTAATT
  421 CCTATCACAA ACTAGGGGAT CTAGCTTGTG GAAGGCTACT CGAAATGTTT GACCCAAGTT
  481 AAACAATTTA AAGGCAATGC TACCAAATAC TAATTGAGTG TATGTAAAAC TTCTGACCCA
  541 CTGGGAATGT GATGAAAGAA ATAAAAGCTG AAAATGAATC ATTCTCTCTA CTATTATTCT
  601 GATATTTCAC ATTCTTAAAA TAAAGTGGTG ATCCTAAACT GACCTAAAGA CAGGGAATTT
  661 TTACTAGGAT TAAATGTCAG AATTGTGAAA AAGTGAGTTT AAATGTATTT GGCTAAGGTG
  721 TATGTAAACT TCCGAACTTC AACTGTATAG GGATCCTCTA GCTAGAGTCG ACCTCGAAGG
  781 GGGGGGCCCG GGTACCCCAG CCTTTTGTTC CCTTTTAGTG AAGGGTTAAA TTGGCGCGCT
  841 TTGGGCGTAA ATCATGGGTC AATAACCTGG TTTCCTGTGT GAAAATTGTA ATCCGCCTCA
  901 CAAATTCCCA CCAACAAACA ATACCGAAGC CACGGAAAGC CATAAAAGTT GTAAAAGCCT
  961 TGGGGTTGCC CTTAAATGAA GGTGGAGCCT AACCTCACCA ATTAAAATTG GCCGTTGCCG
 1021 CTCCACTTGC CCCGCCTTTT TCCAAGATTC CGGGAAAAAC CCTTGATCCG TTGGCCAAGC
 1081 CCTGCCCAAT AAAATGGAAT TCCGGCCAAA ACGTCGCCGG GAGAAAAGAG CCGGTTATAG
1141 GCCGATTATA TTGGGGCGAC G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sumiyoshi, K., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [link to RRC of NBRP]