Resource data sheet
Prev.

pMXs-SB11-IRES-puro

Retroviral expression vector of fish Sleeping Beauty 11 (SB11) transposase in mammalian cells.

Catalog number RDB16060
Resource name pMXs-SB11-IRES-puro
Alternative name Sleeping Beauty (SB) vector
Clone info. Retroviral expression vector of fish Sleeping Beauty 11 (SB11) transposase in mammalian cells.
Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pMXs‐IRES‐Puro (plasmid)
Size of vector backbone 5.8 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Salmon Sleeping Beauty transposase cDNA
Depositor Koso, Hideto |

Distribution information

Ordering This clone is provided directly from Dr. Hideto Koso of the Institute of Medical Science, Tokyo University.
Distribution information (domestic users) [open/close]



References and tips

Electronic file

Featured content

References

original Sumiyoshi, K., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16060_A8Idp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F2 RDB16060_A8Ida.seq
>D05854A1-2_A8Id_MMLV-pol_F2_F02_17_ABI24.ab1
    1 TCGAGGGGGG GTTTGAATTC CTTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCA
   61 CCATGGGAAA ATCAAAAGAA ATCAGCCAAG ACCTCAGAAA AAAAATTGTA GACCTCCACA
  121 AGTCTGGTTC ATCCTTGGGA GCAATTTCCA AACGCCTGAA AGTACCACGT TCATCTGTAC
  181 AAACAATAGT ACGCAAGTAT AAACACCATG GGACCACGCA GCCGTCATAC CGCTCAGGAA
  241 GGAGACGCGT TCTGTCTCCT AGAGATGAAC GTACTTTGGT GCGAAAAGTG CAAATCAATC
  301 CCAGAACAAC AGCAAAGGAC CTTGTGAAGA TGCTGGAGGA AACAGGTACA AAAGTATCTA
  361 TATCCACAGT AAAACGAGTC CTATATCGAC ATAACCTGAA AGGCCGCTCA GCAAGGAAGA
  421 AGCCACTGCT CCAAAACCGA CATAAGAAAG CCAGACTACG GTTTGCAAGA GCACATGGGG
  481 ACAAAGATCG TACTTTTTGG AGAAATGTCC TCTGGTCTGA TGAAACAAAA ATAGAACTGT
  541 TTGGCCATAA TGACCATCGT TATGTTTGGA GGAAGAAGGG GGAGGCTTGC AAGCCGAAGA
  601 ACACCATCCC AACCGTGAAG CACGGGGGTG GCAGCATCAT GTTGTGGGGG TGCTTTGCTG
  661 CAGGAGGGAC TGGTGCACTT CACAAAATAG ATGGCATCAT GAGGAAGGAA AATTATGTGG
  721 ATATATTGAA GCAACATCTC AAGACATCAG TCAGGAAGTT AAAGCTTGGT CGCAAATGGG
  781 TCTTCCAACA AGACAATGAC CCCAAGCATA CTTCCAAACA CGTGAGAAAA TGGCTTAAGG
  841 ACAACAAAGT CAAAGGTATT GGAGTGGCCA TCACAAAGCC CTGACCTCAA TCCTATAGAA
  901 AATTTGTGGG CAGAACTGAA AAAAGCGTGT GCGAGCAAAG GAGGCCTACA AACCCTGACT
  961 CAGTTTACAC CCAGCTCTGT CAGGAGAAAT GGGCCCAAAT TCACCCAACT TATGTGGAAG
 1021 CTTGTGGCAA GGCTACCGAA CCGTTTGACC AAGTCGACCA ATTTAAAGTC AATGCTACCT
1081 AAT
//
Primer: CSII-CMV3' RDB16060_A8Idb.seq
>D05854A1-2_A8Id_CSII-CMV3'_G02_20_ABI24.ab1
    1 GGGCGGATAG TAGCGGCCGC GGCGCGCCGG CCCTCGAGGC CTGCAGGAAT TCGCCCTTCT
   61 AGTATTTGGT AGCATTGCCT TTAAATTGTT TAACTTGGGT CAAACGTTTC GGGTAGCCTT
  121 CCACAAGCTT CCCACAATAA GTTGGGTGAA TTTTGGCCCA TTCCTCCTGA CAGAGCTGGT
  181 GTAACTGAGT CAGGTTTGTA GGCCTCCTTG CTCGCACACG CTTTTTCAGT TCTGCCCACA
  241 AATTTTCTAT AGGATTGAGG TCAGGGCTTT GTGATGGCCA CTCCAATACC TTGACTTTGT
  301 TGTCCTTAAG CCATTTTCTC ACGTGTTTGG AAGTATGCTT GGGGTCATTG TCTTGTTGGA
  361 AGACCCATTT GCGACCAAGC TTTAACTTCC TGACTGATGT CTTGAGATGT TGCTTCAATA
  421 TATCCACATA ATTTTCCTTC CTCATGATGC CATCTATTTT GTGAAGTGCA CCAGTCCCTC
  481 CTGCAGCAAA GCACCCCCAC AACATGATGC TGCCACCCCC GTGCTTCACG GTTGGGATGG
  541 TGTTCTTCGG CTTGCAAGCC TCCCCCTTCT TCCTCCAAAC ATAACGATGG TCATTATGGC
  601 CAAACAGTTC TATTTTTGTT TCATCAGACC AGAGGACATT TCTCCAAAAA GTACGATCTT
  661 TGTCCCCATG TGCTCTTGCA AACCGTAGTC TGGCTTTCTT ATGTCGGTTT TGGAGCAGTG
  721 GCTTCTTCCT TGCTGAGCGG CCTTTCAGGT TATGTCGATA TAGGACTCGT TTTACTGTGG
  781 ATATAGATAC TTTTGTACCT GTTTCCTCCA GCATCTTCAC AAGGTCCTTT GCTGTTGTTC
  841 TGGGATTGAT TTGCACTTTT CGCACCAAAG TACGTTCATC TCTAGGAGAC AGAACGCGTC
  901 TCCTTCCTGA GCGGTATGAC GGCTGCGTGG TCCCATGGGT GTTTATACTT GCGTACTATT
  961 GTTTGTACAG ATGAAACGTG GTACTTTCAG GCGTTTGGGA AAATTGGCTC CCAACGATGG
 1021 AACCAGACTT GTTGAGTCTA CAATTTTTTT TCTGAAGTCC TGCTGGATTT TCCTTTTGAA
1081 TTTTCCCC
//
Primer: IRES-F RDB16060_A8Idc.seq
>D05854A1-2_A8Id_IRES-F_H02_23_ABI24.ab1
    1 GCAGCTTTCA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTTA CCGAGTACAA
  121 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC
  181 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG
  241 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG
  301 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG
  361 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC
  421 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT
  481 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT
  541 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC
  601 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC
  661 CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAGTCG ACGATAAAAT
  721 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG
  781 GCAAGCTAGC TTAAGTAACG CCATTTTGCA AAGGCATGGA AAAATACATA ACTGAGAATA
  841 GAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC CAAACAGGAT
  901 ATCTGTGGGT AAGCAGTTTC CTGCCCCGGG CTCATGGCCC AAGAAACAGA TGGACAGCTG
  961 ATATGGGCCA AACCAGATAT TCTGTGGTAG CAGTTCTTGC CTCACTCAAG ACAGACAGAT
1021 GATCCCCAGA TGCCGATCAG TCTCAGTCAG ATTCTAGAAC CTATCAGATG GATA
//
Primer: PuroR_F RDB16060_A8Idd.seq
>D05854A1-2_A8Id_PuroR_F_C01_03_ABI08.ab1
    1 TTGCGTTTTC GGCCGGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC
   61 AAGCCCGGTG CCTGAGTCGA CGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG
  121 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA
  181 GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTCAG GAACAGATGG
  241 AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG
  301 GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC
  361 CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT
  421 TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT
  481 ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC
  541 TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC
  601 CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG
  661 TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCACATGC
  721 AGCATGTATC AAAATTAATT TGGTTTTTTT TCTTAAGTAT TTACATTAAA TGGCCATAGT
  781 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
  841 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
  901 ACTCAAAGGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
  961 TGAGCAAAAG GCCAGCAAAA GGCCAGGGAA CCCGTAAAAA GGCCGCGTTG CTGGGCGTTT
 1021 TTTCCATAGG CTCCGCCCCC CCTGACGAGC ATCACAAAAA AATCGACGCT CAAGGTCAGA
 1081 GTGGCGAAAC CCGAACAAGG GACTTATAAG AATACCAGGC GTTTCCCCTG AGCTCCATCG
1141 GTGGCGCTTC TCTTGTTCCG GACTCTGTGC CGGCCTTAAC CGGAAATACC CCTGTGATC
//
Primer: Amp_R RDB16060_A8Ide.seq
>D05854A1-2_A8Id_Amp_R_A01_01_ABI08.ab1
    1 GCTTTTTTTT CATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT
  181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA
  241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
  301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
  361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC
  421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT
  481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC
  541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT
  601 GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT
  661 CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT
  721 CATTTGGGGG CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG
  781 GGAGGTAAGC TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA
  841 TTTTATGCGC CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG
  901 TGGAACTGAC GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTCGG
  961 GGGCCGTTTT TGTGGCCCGA CCTGAGTCCA AAAATCCCGA TCGTTTTGAC TCTTTGGGTG
 1021 CACCCCCCTA ATAGGAGGGA TATGTGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC
 1081 CGCCTCCGTC TGAATTTTTG CTTTCGGTTG GACCGAAGCG CGTCGCGCGA TCCTGGTCTG
1141 GCTGCCAGCC ATCGATTCGT GATGATGGAT CTCCCTGGTT CCTGGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sumiyoshi, K., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648.

2019.03.27

GNP_filter3_RDBDEP_html_190327.pl