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pMXs-SB11-IRES-puro (#RDB16060)

Retroviral expression vector of fish Sleeping Beauty 11 (SB11) transposase in mammalian cells.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sumiyoshi, K., Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name Sleeping Beauty (SB) vector
Clone info. Retroviral expression vector of fish Sleeping Beauty 11 (SB11) transposase in mammalian cells.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pMXs‐IRES‐Puro (plasmid)
Size of vector backbone 5.8 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Salmon Sleeping Beauty transposase cDNA
Depositor|Developer Koso, Hideto |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018).
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB16060 pMXs-SB11-IRES-puro DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMXs-SB11-IRES-puro was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16060).

Reference section:

Sumiyoshi, K., Koso, H., Watanabe, S., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sumiyoshi, K., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [PubMed] [Article] [RRC of NBRP]