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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Erg6-FLAG-GFP11

Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) for visualizing organelle contact sites in vivo.

Catalog number RDB16054
Resource name Erg6-FLAG-GFP11
Alternative name pRS314-GPDp-Erg6-3xFLAG-GFP11 (pSFL67)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae ERG6 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae ERG6

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16054 Erg6-FLAG-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16054zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16054_A8I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro)
          Sequence file: RDB16054_A8I3e.seq check
          >16054_16054_B0JcF_HB-NOS_R_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN AANTTGGNNN NNNCCNGGGT TTTCCCAGTC ACGACGTTGT
             61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA
            121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT
            181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA
            241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC
            301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA
            361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG
            421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG
            481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT
            541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAN TTGGAAAAAG CTGAAAAAAA
            601 AGGNTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGANNG
            661 NANGNANTGA NTGNAATTCT GNNNNNNATT TCNNNNNTTC NTAAATTCNN NTTTNNNANN
          721 NAGTCTTTTT TTTNNNNTTT NNAANNNNNN NNNN
          //
          Primer: SV40pA3'Element Reverse
          Region: T3 pro, insert 3' (CYC1 ter)
          Sequence file: RDB16054_A8I3k.seq check
          >D05803_B9_A8I3_1_SV40pA3'element Reverse_F07_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGNNANGCN NGCAACGCGG CCTTTTNACG GTTCCTGGCC
             61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
            121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
            181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
            241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
            301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG
            361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC
            421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA
            481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT
            541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA
            601 CGTTCTTAAT ACTAACATAA CTATAAAAAA ATAAATAGGG ACCTAGACTT CAGGTTGTCT
            661 AACTCCTTCC TTTTCGGTTA GAGCGGATGT GGGGGGANGG CGTGAATGTA AGCGTGACAT
            721 AACTAATTAC ATGACTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCTTATGTT
            781 ATCCCTGCAG CATTGNCGTA TTCATGCAAA ACCATGTGNT CTCTGGAAGT TGAACCTCCA
            841 CCACTTCCGC CTTTANCGNC ATCATCCTTA TANNCAANNN CATGNNNCTT ATAATCGCNN
          901 TCANGNNNTT NNTANNCACN NCCGGNNNNN NNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP11, 3xFLAG, Erg6 3')
          Sequence file: RDB16054_A8I3l.seq check
          >D05803_B9_A8I3_1_CYC1ter_R_C12_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNCN GNNGTGGGGG GNNGGCGTGA ANGTAAGCGT GACATAACTA
             61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC
            121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT
            181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG
            241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTGAGTTGCT TCTTGGGAAG TTTGGGAGGG
            301 GGTTTCGGCG TTTTCTGGCT TCCTAGCGAC GAAAAGCATC ATTGGAGTGA ATAACTTGGA
            361 CTTACCACCG GCAACTAAAC CAACCGCAGC ATTTTCTAGA GCAGCAGTAA CTTCCTTGGA
            421 ACCTTCTGGG GCTAGACCTA ATTTTTCCAT TACAGTAACC ATTGCTGTAG TAAATTGTCT
            481 ACCCAAGTAA GAAGTTCTGA AAAATGTGGC CAAATTAGCT AAGTTTTGAA CGTACTTCCA
            541 CTCACCAGTT AATGGGTAAT ACCAAGGGAT TTCATCATCA TTGTCCGCCA GGTCTTCGCT
            601 AACGAGGACT TCGAAACCAC AGTTCTTCAA TGCTTTCCTA GCCACGTCGA CATGGAACAT
            661 CTTTGGGATA CCATCACCTA GTTCAATTTC ATAAGCGATC TTTCTATGTT CAGGATTGTT
            721 TTCGTCATAT TTATCAGTCA TTACCCATTC GTAAACAGCA AAGGTACCAC CCGGTTTCNA
            781 AACCTTGTAG ATTTCGCTGT ATACACCTTC TAATTTTGGA NCGTGNCATG TGGCCTCANT
            841 TGCATAAACT TTGTCGAAAG NGTTTTCTNC NAANTNNATT TTNCATGAAN TCACCNNTNN
          901 NNAANNNCAN TTNNGNCANT CNAANNNNNN TTTTTNNNNN NNNNN
          //
          Primer: TRP1pro_F
          Region: TRP1
          Sequence file: RDB16054_A8I3m.seq check
          >D05803_B9_A8I3_1_TRP1prp_F_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCTTNNNN NNTGTCTGTT ATTAATTTNN
             61 CNNGGTAGTT CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA
            121 TGTGCTCTAG ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA
            181 ACAATTGACC CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT
            241 TCAGGCACTC CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG
            301 GCTCTGGTCA ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA
            361 GAATACCAAG AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC
            421 AACATACTAC TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA
            481 GAAGCAGGTG GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG
            541 CAAGAGAGCC CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT
            601 GGTGATGCGC TTAGATTAAA TGGCGTTATT GGTGNNNNNN TAAGCGGAGG TGTGGAGACA
            661 AATGGTGTAA AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT
            721 ATTACTGANN AGTATTTATT TANGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA
            781 ATACCGCACA NATGCGTAAG GAGAAAAATA CCGCATCANG GAAATTGNAA GCGTTAATAT
            841 TTTGTNNANT TCGCGTTNAA NNTTTTGTTN AATCAGCTCA NTTTTTANCC NANANGCCNA
            901 AANNNGNNAA ANNCCTTATA AATCNAAANA NNAGANCGAN NNNNNGGTTG ANTGNTNNNN
            961 NCNNTTNGGA NNAGAGTNCN CTATNNANNN NNCNNGNANN NNNNNGTCNA ANGNCNAAAA
          1021 ACCGTNNATC NNGGNNANNN NNNNNACNNN NNNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Erg6 5')
          Sequence file: RDB16054_A8I3n.seq check
          >16054_16054_B0JcF_TDH3pro_F_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNGNNTGNN NNNCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT
             61 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC GGATGCGGCC
            121 GCATGAGTGA AACAGAATTG AGAAAAAGAC AGGCCCAATT CACTAGGGAG TTACATGGTG
            181 ATGATATTGG TAAAAAGACA GGTTTGAGTG CATTGATGTC GAAGAACAAC TCTGCCCAAA
            241 AGGAAGCCGT TCAGAAGTAC TTGAGAAATT GGGATGGTAG AACCGATAAA GATGCCGAAG
            301 AACGTCGTCT TGAGGATTAT AATGAAGCCA CACATTCCTA CTATAACGTC GTTACAGATT
            361 TCTATGAATA TGGTTGGGGT TCCTCTTTCC ATTTCAGCAG ATTTTATAAA GGTGAGAGTT
            421 TCGCTGCCTC GATAGCAAGA CATGAACATT ATTTAGCTTA CAAGGCTGGT ATTCAAAGAG
            481 GCGATTTAGT TCTCGACGTT GGTTGTGGTG TTGGGGGCCC AGCAAGAGAG ATTGCAAGAT
            541 TTACCGGTTG TAACGTCATC GGTCTAAACA ATAACGATTA CCAAATTGCC AAGGNAAAAT
            601 ATTACGCTAA AAAATACAAT TTGAGTGANC AAATGGACTT TGTAAAGGGT GANTTCATGA
            661 AAATGGNNTT CNAAGANNNN CTTTCNANNA NNTTTATGCN NTTGNNNNNC ATGNNNCGCT
          721 NCNAANNNNN NANNNNGNNN NNNNNNN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB16054_A8I3j.seq check
          >D05803_B9_A8I3_1_Amp_R_H07_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGCANTTAT NAGGGTTATT GTCTCNTGAG CGGATACATA
             61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
            121 CCACCTGGGT CCTTTTCATC ACGTGCTATA AAAATAATTA TAATTTAAAT TTTTTAATAT
            181 AAATATATAA ATTAAAAATA GAAAGTAAAA AAAGAAATTA AAGAAAAAAT AGTTTTTGTT
            241 TTCCGAAGAT GTAAAAGACT CTAGGGGGAT CGCCAACAAA TACTACCTTT TATCTTGCTC
            301 TTCCTGCTCT CAGGTATTAA TGCCGAATTG TTTCATCTTG TCTGTGTAGA AGACCACACA
            361 CGAAAATCCT GTGATTTTAC ATTTTACTTA TCGTTAATCG AATGTATATC TATTTAATCT
            421 GCTTTTCTTG TCTAATAAAT ATATATGTAA AGTACGCTTT TTGTTGAAAT TTTTTAAACC
            481 TTTGTTTATT TTTTTTTCTT CATTCCGTAA CTCTTCTACC TTCTTTATTT ACTTTCTAAA
            541 ATCCAAATAC AAAACATAAA AATAAATAAA CACAGAGTAA ATTCCCAAAT TATTCCATCA
            601 TTAAAAGATA CGAGGCGCGT GTAAGTTACA GGCAAGCGAT CCGTCTAAGA AACCATTATT
            661 ATCATGACAT TAACCTATAA AAATAGGCGT ATCNCGANGC CCTTTCGTCT CGCGCGTTNN
            721 GGTGATGANG GTGAAANNCT CTGANACNTG CAGCTNCCNG ANANGGTCAC AGCTNGTCTG
            781 TAAGCGGANG CCGGGANCAN ANANNCCCGT CANGNNNCGT CANNNGGTGT TNNCGGGNNN
            841 NGGGGNNNGN CNNNNCTANN CNNNNTCNNA NCANANTNGN NCTGANNNNN NNACCNNNNN
          901 NNNCGANNNN NCNNNNTNTN TNNNNNNNNG NNNNNNNTNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl