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Erg6-FLAG-GFP11

Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) for visualizing organelle contact sites in vivo.

Catalog number RDB16054
Resource name Erg6-FLAG-GFP11
Alternative name pRS314-GPDp-Erg6-3xFLAG-GFP11 (pSFL67)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae ERG6 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae ERG6 (KEGG orthology K00559) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB16054 Erg6-FLAG-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16054_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB16054_A8I3a.seq check
>D05803_B9_A8I3_1_M13_-40_E07_13_ABI24.ab1
    1 AGCAAGGATG TATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC ATTATCAATA
   61 CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA CTTTATTTAG
  121 TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA GGGGGCGGGT
  181 TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG GCATCCACTA
  241 AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC CCAGCACCAA
  301 AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG CAACTACAGA
  361 GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT GCAACCTGCC
  421 TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA TTTTCTTACA
  481 CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG GTTGAAACCA
  541 GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA GGTATTGATT
  601 GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT TAGTCTTTTT
  661 TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCATGAG TGAAACAGAA
  721 TTGAGAAAAA GACAGGCCCA ATTCACTAGG GAGTTACATG GTGATGATAT TGGTAAAAAG
  781 ACAGGTTTGA GTGCATTGAT GTCGAAGAAC AACTCTGCCC AAAAGGAAGC CGTTCAGAAG
  841 TACTTGAGAA ATTGGGATGG TAGAACCGAT AAAGATGCCG AAGAACGTCG TCTTGAGGAT
  901 TATAATGAAG CCACACATTC CTACTATAAC GTCGTTACAG ATTTCTATGA ATATGGTTGG
  961 GGGTTTCCTC TTTCCCATTT CAGCAGATTT TTATAAACGT GAGAGTTTTC GCCTGCTCGA
 1021 TAGCAGACAT GAACAATAAT TTAGCCTACA AGCTGGTATC AAGAAGCGAA TTAGGTCTCG
1081 GACGATTGGG ATAGA
//
Primer: CYC1ter_R
Sequence file: RDB16054_A8I3b.seq check
>D05803_B9_A8I3_1_CYC1ter_R_C12_09_ABI24.ab1
    1 ATGTCGTTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC
  121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT
  181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG
  241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTGAGTTGCT TCTTGGGAAG TTTGGGAGGG
  301 GGTTTCGGCG TTTTCTGGCT TCCTAGCGAC GAAAAGCATC ATTGGAGTGA ATAACTTGGA
  361 CTTACCACCG GCAACTAAAC CAACCGCAGC ATTTTCTAGA GCAGCAGTAA CTTCCTTGGA
  421 ACCTTCTGGG GCTAGACCTA ATTTTTCCAT TACAGTAACC ATTGCTGTAG TAAATTGTCT
  481 ACCCAAGTAA GAAGTTCTGA AAAATGTGGC CAAATTAGCT AAGTTTTGAA CGTACTTCCA
  541 CTCACCAGTT AATGGGTAAT ACCAAGGGAT TTCATCATCA TTGTCCGCCA GGTCTTCGCT
  601 AACGAGGACT TCGAAACCAC AGTTCTTCAA TGCTTTCCTA GCCACGTCGA CATGGAACAT
  661 CTTTGGGATA CCATCACCTA GTTCAATTTC ATAAGCGATC TTTCTATGTT CAGGATTGTT
  721 TTCGTCATAT TTATCAGTCA TTACCCATTC GTAAACAGCA AAGGTACCAC CCGGTTTCAA
  781 AACCTTGTAG ATTTCGCTGT ATACACCTTC TAATTTTGGA GCGTGACATG TGGCCTCAAT
  841 TGCATAAACT TTGTCGAAAG TGTTTTCTTC GAAATCCATT TTTCATGAAA TCACCCTTTA
  901 CAAAGTCCAT TTTGGTCACT CAAATTGTAT TTTTTTAGCG TAATATTTTT GCCCTTGGAC
  961 AATTTGGGTA AATCGTTTAT TTGTTTAAGA ACCGATGACG TTTACACCCG GTTAAATTCC
 1021 TTGCCATCCT CCTCTTGGCT GGGCCCCCAC AACGACACCG AACGATCGAG CACTAATTCG
1081 ACGTCTTTGA ACTAC
//
Primer: TRP1pro_F
Sequence file: RDB16054_A8I3c.seq check
>D05803_B9_A8I3_1_TRP1prp_F_F01_06_ABI08.ab1
    1 GAATTAGGGT TGAATTTAAC CCACAAAGGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA
   61 CAGGGTAGTT CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA
  121 TGTGCTCTAG ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA
  181 ACAATTGACC CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT
  241 TCAGGCACTC CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG
  301 GCTCTGGTCA ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA
  361 GAATACCAAG AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC
  421 AACATACTAC TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA
  481 GAAGCAGGTG GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG
  541 CAAGAGAGCC CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT
  601 GGTGATGCGC TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA
  661 AATGGTGTAA AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT
  721 ATTACTGAGT AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA
  781 ATACCGCACA GATGCGTAAG GAGAAAAATA CCGCATCAGG GAAATTGTAA GCGTTAATAT
  841 TTTGTTAAAT TCGCGTTAAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA
  901 AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG GATAGGGTTG AGTGTTGGTT
  961 CCAGTTTGGA CAAGAGTCCA CTATTAAAGG AACGTGGACT CCAACGTCAA AGGGCGAAAA
 1021 ACCGTCTATC AGGGCGATGG CCACTACGTG AACCATCACC TAATCAAGGT TTTTTGGGGG
 1081 TCGAGGGTTG CGGTAAAGCA CTTAAATCGG AGCCTAAAGG GGAGCCCCCG GAATTAGAGG
1141 CTGGACGGAA AGCCGCACGT GGCGAAGGAC GGGAAGAAAG CGTAAAGGGA GACGGCG
//
Primer: Amp_R
Sequence file: RDB16054_A8I3d.seq check
>D05803_B9_A8I3_1_Amp_R_H07_22_ABI24.ab1
    1 TTTCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGGGT CCTTTTCATC ACGTGCTATA AAAATAATTA TAATTTAAAT TTTTTAATAT
  181 AAATATATAA ATTAAAAATA GAAAGTAAAA AAAGAAATTA AAGAAAAAAT AGTTTTTGTT
  241 TTCCGAAGAT GTAAAAGACT CTAGGGGGAT CGCCAACAAA TACTACCTTT TATCTTGCTC
  301 TTCCTGCTCT CAGGTATTAA TGCCGAATTG TTTCATCTTG TCTGTGTAGA AGACCACACA
  361 CGAAAATCCT GTGATTTTAC ATTTTACTTA TCGTTAATCG AATGTATATC TATTTAATCT
  421 GCTTTTCTTG TCTAATAAAT ATATATGTAA AGTACGCTTT TTGTTGAAAT TTTTTAAACC
  481 TTTGTTTATT TTTTTTTCTT CATTCCGTAA CTCTTCTACC TTCTTTATTT ACTTTCTAAA
  541 ATCCAAATAC AAAACATAAA AATAAATAAA CACAGAGTAA ATTCCCAAAT TATTCCATCA
  601 TTAAAAGATA CGAGGCGCGT GTAAGTTACA GGCAAGCGAT CCGTCTAAGA AACCATTATT
  661 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC
  721 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG
  781 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT
  841 CGGGGCTGGG CTTAACTATG CGGCATCAGA GCAGATTTGT ACTGAGAAGT GCACCATAGA
  901 TCACGACATT ACTATATATA TAATAATAGG GAAGCATTTA ATAGAACAGG CATCGTAAAT
  961 ATAATGTGTA CCTTTTGCAG TATGACGGCC AGGATGGGCC AGTTAGTGGC AAGAATATTC
 1021 TTTTATTGAA AAAATAGGCT GTTCAACTAA CGGTACATCC TGGATCGGAA GCCTTTCCAT
1081 TATGCCGATA GATATTTCGG TCGGAAAAGG AAAAGG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl