Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Erg6-GFP1-10

Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) for visualizing organelle contact sites in vivo.

Catalog number RDB16053
Resource name Erg6-GFP1-10
Alternative name pRS316-GPDp-Erg6-GFP1-10 (pSFL28)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae ERG6 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae ERG6

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16053 Erg6-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16053zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16053_A8I3p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro)
          Sequence file: RDB16053_A8I3e.seq check
          >16053_16053_B0JcF_HB-NOS_R_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNGNGNNNNN NTGGGTAACG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA
             61 ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CAGTTTATCA
            121 TTATCAATAC TCGCCATTTC AAAGAATACG TAAATAATTA ATAGTAGTGA TTTTCCTAAC
            181 TTTATTTAGT CAAAAAATTA GCCTTTTAAT TCTGCTGTAA CCCGTACATG CCCAAAATAG
            241 GGGGCGGGTT ACACAGAATA TATAACATCG TAGGTGTCTG GGTGAACAGT TTATTCCTGG
            301 CATCCACTAA ATATAATGGA GCCCGCTTTT TAAGCTGGCA TCCAGAAAAA AAAAGAATCC
            361 CAGCACCAAA ATATTGTTTT CTTCACCAAC CATCAGTTCA TAGGTCCATT CTCTTAGCGC
            421 AACTACAGAG AACAGGGGCA CAAACAGGCA AAAAACGGGC ACAACCTCAA TGGAGTGATG
            481 CAACCTGCCT GGAGTAAATG ATGACACAAG GCAATTGACC CACGCATGTA TCTATCTCAT
            541 TTTCTTACAC CTTCTATTAC CTTCTGCTCT CTCTGANTTG GAAAAAGCTG AAAAAAAAGG
            601 TTGAAACCAG TTCCCTGAAA TTATTCCCCT ACTTGACTAA TAAGTATATA AAGACGGTAN
            661 GTATTGATTG NAATTCTGNN NNCNATTTCT NNNNTTCNTN NNNNNNACTT TTANNGTNNN
          721 NCNTTTTTTT NNNNTTNNAA NNNNNNNNNN
          //
          Primer: SV40pA3'Element Reverse
          Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
          Sequence file: RDB16053_A8I3f.seq check
          >D05803_B8_A8I3_2_SV40pA3'element Reverse_B07_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGGNANNG CNNGCAACGC GGCCTTTTTA CGGTTCCTGG
             61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
            121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
            181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
            241 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG
            301 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA
            361 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA
            421 GCTATGACCA TGATTACGCC AAGCTCGAAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
            481 TACCGGCCGC AAATTAAAGC CTTCGAGCGT CCCAAAACCT TCTCAAGCAA GGTTTTCAGT
            541 ATAATGTTAC ATGCGTACAC GCGTCTGTAC AGAAAAAAAA GAAAAATTTG AAATATAAAT
            601 AACGTTCTTA ATACTAACAT AACTATAAAA AAATAAATAG GGACCTAGAC TTCAGGTTGT
            661 CTAACTCCTT CCTTTTCGGT TAGAGCGGAT GTGGGGGGAG GGCGTGAATG TAAGCGTGAC
            721 ATAACTAATT ACATGACTCG AGGTCGACGG TATCGATAAG CTTGATATCG AATTCTTACT
            781 TTTCGTTGGG NTCTTTCGAA AGGANAGTTT GTGTCGACAG GTAATGGTTG TCTGGNAAAA
            841 NGANAGGGCN ATCGCCNATN GGANNATTTT NGTTGATNAN NGNCNGCNAN TNNNNCGGAA
          901 NNNTCTNNNA NNTNNNNGNN ANNNNNNNNN N
          //
          Primer: paGFP-M_R1
          Region: insert mid (GFP1-10 5', Erg6 3')
          Sequence file: RDB16053_A8I3g.seq check
          >16053_16053_B0JcF_paGFP-M_R1_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NNTTTNNTGT GNNCCGGANA ACGGGAAAAG CATTGAACAC
             61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA
            121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA
            181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA
            241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC
            301 CTTGAGTTGC TTCTTGGGAA GTTTGGGAGG GGGTTTCGGC GTTTTCTGGC TTCCTAGCGA
            361 CGAAAAGCAT CATTGGAGTG AATAACTTGG ACTTACCACC GGCAACTAAA CCAACCGCAG
            421 CATTTTCTAG AGCAGCAGTA ACTTCCTTGG AACCTTCTGG GGCTAGACCT AATTTTTCCA
            481 TTACAGTAAC CATTGCTGTA GTAAATTGTC TACCCAAGTA AGAAGTTCTG AAAAATGTGG
            541 CCAAATTAGC TAAGTTTTGA ACGTACTTCC ACTCACCAGT TAATGGGTAA TACCAAGGGA
            601 NTTCATCATC ATTGTCCGCC AGGTCTTCGC TAACGANGAN TTCNAAACCN CAGTTCNTCN
            661 ATGNTTTCCN ANCCNCNNCG ANNTNNNNNA TCTTTGGGAN ACNNTCANNN ANNNNNANTN
          721 NNNNNNNNN
          //
          Primer: URA3pro_F
          Region: URA3 pro, URA3
          Sequence file: RDB16053_A8I3h.seq check
          >D05803_B8_A8I3_2_URA3pro_F_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNTNNN NANNNTGAAA TTGCCNNGTA TTCTTAACCC NACTGCACAG
             61 AACAAAAACC TGCAGGAAAC GAAGATAAAT CATGTCGAAA GCTACATATA AGGAACGTGC
            121 TGCTACTCAT CCTAGTCCTG TTGCTGCCAA GCTATTTAAT ATCATGCACG AAAAGCAAAC
            181 AAACTTGTGT GCTTCATTGG ATGTTCGTAC CACCAAGGAA TTACTGGAGT TAGTTGAAGC
            241 ATTAGGTCCC AAAATTTGTT TACTAAAAAC ACATGTGGAT ATCTTGACTG ATTTTTCCAT
            301 GGAGGGCACA GTTAAGCCGC TAAAGGCATT ATCCGCCAAG TACAATTTTT TACTCTTCGA
            361 AGACAGAAAA TTTGCTGACA TTGGTAATAC AGTCAAATTG CAGTACTCTG CGGGTGTATA
            421 CAGAATAGCA GAATGGGCAG ACATTACGAA TGCACACGGT GTGGTGGGCC CAGGTATTGT
            481 TAGCGGTTTG AAGCAGGCGG CAGAAGAAGT AACAAAGGAA CCTAGAGGCC TTTTGATGTT
            541 AGCAGAATTG TCATGCAAGG GCTCCCTATC TACTGGAGAA TATACTAAGG GTACTGTTGA
            601 CATTGCGAAG AGCGACAAAG ATTTTGTTAT CGGCTTTATT GCTCAAAGAG ACATGGGTGG
            661 AAGAGATGAA GGTTACGATT GGTTGATTAT GACACCCGGT GTGGGTTTAG ATGACAAGGG
            721 AGACGCATTG GGTCAACAGT ATAGAACCGT GGATGATGTG GTCTCTACAG GATCTGACAT
            781 TATTATTGTT GGAAGAGGAC TATTTGCAAA NGGAAGGGAN GCTAANGTAG AGGGTGAACG
            841 TTACAGAAAA GCANGCTGGG AAGCATATTT GANANGATGC GGCCNGCAAA ACTNAAAAAC
            901 TGTATTATAA GTAAATGNAT GTATACTAAA CTCACNAATT AGAGCTNNNN NTTAATTATA
            961 TCNGTTATTA CCCNATGCNG NNGNGAAATA NNNNANAGAN GCNNNANNNA NAAAANNNCN
          1021 NANCNNNAAA NNNNNANNNN NNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Erg6 5')
          Sequence file: RDB16053_A8I3i.seq check
          >16053_16053_B0JcF_TDH3pro_F_D02_11_ABI24.ab1
              1 NNNNNNNGNN NTGNNTGNAN NTCTGTAAAT CTATTTCTTA AACTTCTTAA ATTCTACTTT
             61 TATAGTTAGT CTTTTTTTTA GTTTTAAAAC ACCAGAACTT AGTTTCGACG GATGCGGCCG
            121 CATGAGTGAA ACAGAATTGA GAAAAAGACA GGCCCAATTC ACTAGGGAGT TACATGGTGA
            181 TGATATTGGT AAAAAGACAG GTTTGAGTGC ATTGATGTCG AAGAACAACT CTGCCCAAAA
            241 GGAAGCCGTT CAGAAGTACT TGAGAAATTG GGATGGTAGA ACCGATAAAG ATGCCGAAGA
            301 ACGTCGTCTT GAGGATTATA ATGAAGCCAC ACATTCCTAC TATAACGTCG TTACAGATTT
            361 CTATGAATAT GGTTGGGGTT CCTCTTTCCA TTTCAGCAGA TTTTATAAAG GTGAGAGTTT
            421 CGCTGCCTCG ATAGCAAGAC ATGAACATTA TTTAGCTTAC AAGGCTGGTA TTCAAAGAGG
            481 CGATTTAGTT CTCGACGTTG GTTGTGGTGT TGGGGGCCCA GCAAGAGAGN TTGCAAGATT
            541 TACCGGTTGT AACGTCATCG GTCTAAACAA TAACGATTAC CAAATTGCCN AGGCAAAATA
            601 TTACGCTAAA AAATACNATT TGAGTGACCN AATGGACTTT GTAAAGGGTG ANTTCATGAA
            661 AATGGANTTC NAAGANANAC TTTCNANNAN GTTTNTGCNN TTGNNNNNCN TGNCACGCTN
          721 NNAANNTNNN NNNNGNNNNN NNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP1-10 3')
          Sequence file: RDB16053_A8I3j.seq check
          >D05803_B8_A8I3_2_CYC1ter_R_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGNCATAACT
             61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
            121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
            181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
            241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
            301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
            361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
            421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
            481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
            541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
            601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
            661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
            721 CAACTCCAGT GANAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
            781 CAGACCCTCC GGATCCTTGA NTTGCTTCTT GGGAAGTTTG GGANGGGGTT TCGGCGTTTT
            841 CTGNNTTCCT ANCGACNAAA AGCATCATNN NNNNNNATAA CTNGGACTNA CNNCNNGNNN
          901 NNNAACCNAC CNCNNCANTT NNCNNNNNCN NNNNNNNCNN NCNNNNNNNN NN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB16053_A8I3k.seq check
          >D05803_B8_A8I3_2_Amp_R_D07_10_ABI24.ab1
              1 NNNNNNNNNN NNNNATNNNN CANTNATNNG GGTTATTGTC TCNTGAGCGG ANACATATTT
             61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
            121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
            181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
            241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
            301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
            361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
            421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
            481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
            541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
            601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
            661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
            721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGANA NGGTCACAGC TTGTCTGTAA
            781 GCGGANGCCG GGANCAGACA AGCCCGTCAG GGCGCGTCAG CGGNNGTTGG CGGGNGTCGG
            841 GGCTGGCTTN ACTATGCGGC ATCAGANCAG ANTGNACTGA GAGTGCACCA TANCACCNTT
            901 TCNNNNCNTC ANTTTTTTTT TTTNANNCNT TTTTTTTTNA NTTNNGNTNN CNNTNNNAAN
          961 TTTTTTTNNN NTNNNNNNAN NNNNCNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl