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Erg6-GFP1-10

Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) for visualizing organelle contact sites in vivo.

Catalog number RDB16053
Resource name Erg6-GFP1-10
Alternative name pRS316-GPDp-Erg6-GFP1-10 (pSFL28)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae ERG6 cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae ERG6 (KEGG orthology K00559) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
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RDB16053 Erg6-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16053_A8I3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB16053_A8I3e.seq check
>16053_16053_B0JcF_HB-NOS_R_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNGNGNNNNN NTGGGTAACG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA
   61 ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CAGTTTATCA
  121 TTATCAATAC TCGCCATTTC AAAGAATACG TAAATAATTA ATAGTAGTGA TTTTCCTAAC
  181 TTTATTTAGT CAAAAAATTA GCCTTTTAAT TCTGCTGTAA CCCGTACATG CCCAAAATAG
  241 GGGGCGGGTT ACACAGAATA TATAACATCG TAGGTGTCTG GGTGAACAGT TTATTCCTGG
  301 CATCCACTAA ATATAATGGA GCCCGCTTTT TAAGCTGGCA TCCAGAAAAA AAAAGAATCC
  361 CAGCACCAAA ATATTGTTTT CTTCACCAAC CATCAGTTCA TAGGTCCATT CTCTTAGCGC
  421 AACTACAGAG AACAGGGGCA CAAACAGGCA AAAAACGGGC ACAACCTCAA TGGAGTGATG
  481 CAACCTGCCT GGAGTAAATG ATGACACAAG GCAATTGACC CACGCATGTA TCTATCTCAT
  541 TTTCTTACAC CTTCTATTAC CTTCTGCTCT CTCTGANTTG GAAAAAGCTG AAAAAAAAGG
  601 TTGAAACCAG TTCCCTGAAA TTATTCCCCT ACTTGACTAA TAAGTATATA AAGACGGTAN
  661 GTATTGATTG NAATTCTGNN NNCNATTTCT NNNNTTCNTN NNNNNNACTT TTANNGTNNN
721 NCNTTTTTTT NNNNTTNNAA NNNNNNNNNN
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
Sequence file: RDB16053_A8I3f.seq check
>D05803_B8_A8I3_2_SV40pA3'element Reverse_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGGNANNG CNNGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
  121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
  181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
  241 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG
  301 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA
  361 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA
  421 GCTATGACCA TGATTACGCC AAGCTCGAAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
  481 TACCGGCCGC AAATTAAAGC CTTCGAGCGT CCCAAAACCT TCTCAAGCAA GGTTTTCAGT
  541 ATAATGTTAC ATGCGTACAC GCGTCTGTAC AGAAAAAAAA GAAAAATTTG AAATATAAAT
  601 AACGTTCTTA ATACTAACAT AACTATAAAA AAATAAATAG GGACCTAGAC TTCAGGTTGT
  661 CTAACTCCTT CCTTTTCGGT TAGAGCGGAT GTGGGGGGAG GGCGTGAATG TAAGCGTGAC
  721 ATAACTAATT ACATGACTCG AGGTCGACGG TATCGATAAG CTTGATATCG AATTCTTACT
  781 TTTCGTTGGG NTCTTTCGAA AGGANAGTTT GTGTCGACAG GTAATGGTTG TCTGGNAAAA
  841 NGANAGGGCN ATCGCCNATN GGANNATTTT NGTTGATNAN NGNCNGCNAN TNNNNCGGAA
901 NNNTCTNNNA NNTNNNNGNN ANNNNNNNNN N
//
Primer: paGFP-M_R1
Region: insert mid (GFP1-10 5', Erg6 3')
Sequence file: RDB16053_A8I3g.seq check
>16053_16053_B0JcF_paGFP-M_R1_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNTTTNNTGT GNNCCGGANA ACGGGAAAAG CATTGAACAC
   61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA
  121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA
  181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA
  241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC
  301 CTTGAGTTGC TTCTTGGGAA GTTTGGGAGG GGGTTTCGGC GTTTTCTGGC TTCCTAGCGA
  361 CGAAAAGCAT CATTGGAGTG AATAACTTGG ACTTACCACC GGCAACTAAA CCAACCGCAG
  421 CATTTTCTAG AGCAGCAGTA ACTTCCTTGG AACCTTCTGG GGCTAGACCT AATTTTTCCA
  481 TTACAGTAAC CATTGCTGTA GTAAATTGTC TACCCAAGTA AGAAGTTCTG AAAAATGTGG
  541 CCAAATTAGC TAAGTTTTGA ACGTACTTCC ACTCACCAGT TAATGGGTAA TACCAAGGGA
  601 NTTCATCATC ATTGTCCGCC AGGTCTTCGC TAACGANGAN TTCNAAACCN CAGTTCNTCN
  661 ATGNTTTCCN ANCCNCNNCG ANNTNNNNNA TCTTTGGGAN ACNNTCANNN ANNNNNANTN
721 NNNNNNNNN
//
Primer: URA3pro_F
Region: URA3 pro, URA3
Sequence file: RDB16053_A8I3h.seq check
>D05803_B8_A8I3_2_URA3pro_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTNNN NANNNTGAAA TTGCCNNGTA TTCTTAACCC NACTGCACAG
   61 AACAAAAACC TGCAGGAAAC GAAGATAAAT CATGTCGAAA GCTACATATA AGGAACGTGC
  121 TGCTACTCAT CCTAGTCCTG TTGCTGCCAA GCTATTTAAT ATCATGCACG AAAAGCAAAC
  181 AAACTTGTGT GCTTCATTGG ATGTTCGTAC CACCAAGGAA TTACTGGAGT TAGTTGAAGC
  241 ATTAGGTCCC AAAATTTGTT TACTAAAAAC ACATGTGGAT ATCTTGACTG ATTTTTCCAT
  301 GGAGGGCACA GTTAAGCCGC TAAAGGCATT ATCCGCCAAG TACAATTTTT TACTCTTCGA
  361 AGACAGAAAA TTTGCTGACA TTGGTAATAC AGTCAAATTG CAGTACTCTG CGGGTGTATA
  421 CAGAATAGCA GAATGGGCAG ACATTACGAA TGCACACGGT GTGGTGGGCC CAGGTATTGT
  481 TAGCGGTTTG AAGCAGGCGG CAGAAGAAGT AACAAAGGAA CCTAGAGGCC TTTTGATGTT
  541 AGCAGAATTG TCATGCAAGG GCTCCCTATC TACTGGAGAA TATACTAAGG GTACTGTTGA
  601 CATTGCGAAG AGCGACAAAG ATTTTGTTAT CGGCTTTATT GCTCAAAGAG ACATGGGTGG
  661 AAGAGATGAA GGTTACGATT GGTTGATTAT GACACCCGGT GTGGGTTTAG ATGACAAGGG
  721 AGACGCATTG GGTCAACAGT ATAGAACCGT GGATGATGTG GTCTCTACAG GATCTGACAT
  781 TATTATTGTT GGAAGAGGAC TATTTGCAAA NGGAAGGGAN GCTAANGTAG AGGGTGAACG
  841 TTACAGAAAA GCANGCTGGG AAGCATATTT GANANGATGC GGCCNGCAAA ACTNAAAAAC
  901 TGTATTATAA GTAAATGNAT GTATACTAAA CTCACNAATT AGAGCTNNNN NTTAATTATA
  961 TCNGTTATTA CCCNATGCNG NNGNGAAATA NNNNANAGAN GCNNNANNNA NAAAANNNCN
1021 NANCNNNAAA NNNNNANNNN NNNNNN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, Erg6 5')
Sequence file: RDB16053_A8I3i.seq check
>16053_16053_B0JcF_TDH3pro_F_D02_11_ABI24.ab1
    1 NNNNNNNGNN NTGNNTGNAN NTCTGTAAAT CTATTTCTTA AACTTCTTAA ATTCTACTTT
   61 TATAGTTAGT CTTTTTTTTA GTTTTAAAAC ACCAGAACTT AGTTTCGACG GATGCGGCCG
  121 CATGAGTGAA ACAGAATTGA GAAAAAGACA GGCCCAATTC ACTAGGGAGT TACATGGTGA
  181 TGATATTGGT AAAAAGACAG GTTTGAGTGC ATTGATGTCG AAGAACAACT CTGCCCAAAA
  241 GGAAGCCGTT CAGAAGTACT TGAGAAATTG GGATGGTAGA ACCGATAAAG ATGCCGAAGA
  301 ACGTCGTCTT GAGGATTATA ATGAAGCCAC ACATTCCTAC TATAACGTCG TTACAGATTT
  361 CTATGAATAT GGTTGGGGTT CCTCTTTCCA TTTCAGCAGA TTTTATAAAG GTGAGAGTTT
  421 CGCTGCCTCG ATAGCAAGAC ATGAACATTA TTTAGCTTAC AAGGCTGGTA TTCAAAGAGG
  481 CGATTTAGTT CTCGACGTTG GTTGTGGTGT TGGGGGCCCA GCAAGAGAGN TTGCAAGATT
  541 TACCGGTTGT AACGTCATCG GTCTAAACAA TAACGATTAC CAAATTGCCN AGGCAAAATA
  601 TTACGCTAAA AAATACNATT TGAGTGACCN AATGGACTTT GTAAAGGGTG ANTTCATGAA
  661 AATGGANTTC NAAGANANAC TTTCNANNAN GTTTNTGCNN TTGNNNNNCN TGNCACGCTN
721 NNAANNTNNN NNNNGNNNNN NNNNN
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, GFP1-10 3')
Sequence file: RDB16053_A8I3j.seq check
>D05803_B8_A8I3_2_CYC1ter_R_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGNCATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
  121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
  181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
  241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
  301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
  361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
  421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
  481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
  541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
  601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
  661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
  721 CAACTCCAGT GANAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
  781 CAGACCCTCC GGATCCTTGA NTTGCTTCTT GGGAAGTTTG GGANGGGGTT TCGGCGTTTT
  841 CTGNNTTCCT ANCGACNAAA AGCATCATNN NNNNNNATAA CTNGGACTNA CNNCNNGNNN
901 NNNAACCNAC CNCNNCANTT NNCNNNNNCN NNNNNNNCNN NCNNNNNNNN NN
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB16053_A8I3k.seq check
>D05803_B8_A8I3_2_Amp_R_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNATNNNN CANTNATNNG GGTTATTGTC TCNTGAGCGG ANACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGANA NGGTCACAGC TTGTCTGTAA
  781 GCGGANGCCG GGANCAGACA AGCCCGTCAG GGCGCGTCAG CGGNNGTTGG CGGGNGTCGG
  841 GGCTGGCTTN ACTATGCGGC ATCAGANCAG ANTGNACTGA GAGTGCACCA TANCACCNTT
  901 TCNNNNCNTC ANTTTTTTTT TTTNANNCNT TTTTTTTTNA NTTNNGNTNN CNNTNNNAAN
961 TTTTTTTNNN NTNNNNNNAN NNNNCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.11.06

GNP_filter3_RDBDEP_html_201104.pl