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Pex3N-FLAG-GFP11

Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 for visualizing organelle contact sites in vivo.

Catalog number RDB16052
Resource name Pex3N-FLAG-GFP11
Alternative name pRS316-GPDp-Pex3N-3xFLAG-GFP11 (pSFL96)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae PEX3 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae PEX3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16052 Pex3N-FLAG-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16052zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16052_A8I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GDP pro)
          Sequence file: RDB16052_A8I3f.seq check
          >16052_16052_B0KqF_HB-NOS_R_G02_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNN NNNNNNNGGT ANGCCNGGGT TTTCCCNGTC ACGACGTTGT
             61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA
            121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT
            181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA
            241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC
            301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA
            361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG
            421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG
            481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT
            541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA
            601 AGGTTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG
            661 TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA
            721 GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCATG
            781 GCCNCAAATC AAAGATCACG TTCGCTTCTG CAGANACATC GAGGAAAGGT ACTCATTTCA
            841 TTGACTGGAA TANCTGCTTT ATTTACCACA NGGNCNGNGG TANNGNTTTT TCGNGAANAG
            901 ATGGTTGNAT NAACAGCANT TACNGNNTAC TGANCNACNC TTCATNNNNN NNANNNCNNN
          961 NNNNCTGNNN GNGNNNANNN NNANNANNNN NNNNNN
          //
          Primer: SV40pA3'element Reverse
          Region: T3 pro, insert 3' (CYC1 ter, 3xFLAG-GFP11 3')
          Sequence file: RDB16052_A8I3g.seq check
          >D05803_B7_A8I3_2_SV40pA3'element Reverse_F06_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG GNNNNNCNNG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
             61 TTGCTGNCCT TTTGCTNNCA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
            121 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
            181 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
            241 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
            301 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
            361 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA
            421 TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGTACC
            481 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA
            541 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG
            601 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA
            661 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA
            721 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTATGTTAT
            781 CCCTGCAGCA TTGACGTATT CATGCAAAAC CATGTGATCT CTGGAANTTN NACCNCCACC
            841 ACTTCCGCCT TTATCGNCAT CATCCTTATA GNCAATGNCA NGNTCCTTAT NANCGCCATC
          901 ANNGNCTTNN NNNTCACNAC NGGNANNNNN NNNNN
          //
          Primer: URA3pro_F
          Region: URA3 pro, URA3
          Sequence file: RDB16052_A8I3h.seq check
          >D05803_B7_A8I3_2_URA3pro_F_G06_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTG ANNTGCCNNG TATTCTTAAC CCAACTGCAC AGAACAAAAA
             61 CCTGCAGGNN NNGAAGANAA ATCATGTCGA AAGCTACATA TAAGGAACGT GCTGCTACTC
            121 ATCCTAGTCC TGTTGCTGCC AAGCTATTTA ATATCATGCA CGAAAAGCAA ACAAACTTGT
            181 GTGCTTCATT GGATGTTCGT ACCACCAAGG AATTACTGGA GTTAGTTGAA GCATTAGGTC
            241 CCAAAATTTG TTTACTAAAA ACACATGTGG ATATCTTGAC TGATTTTTCC ATGGAGGGCA
            301 CAGTTAAGCC GCTAAAGGCA TTATCCGCCA AGTACAATTT TTTACTCTTC GAAGACAGAA
            361 AATTTGCTGA CATTGGTAAT ACAGTCAAAT TGCAGTACTC TGCGGGTGTA TACAGAATAG
            421 CAGAATGGGC AGACATTACG AATGCACACG GTGTGGTGGG CCCAGGTATT GTTAGCGGTT
            481 TGAAGCAGGC GGCAGAAGAA GTAACAAAGG AACCTAGAGG CCTTTTGATG TTAGCAGAAT
            541 TGTCATGCAA GGGCTCCCTA TCTACTGGAG AATATACTAA GGGTACTGTT GACATTGCGA
            601 AGAGCGACAA AGATTTTGTT ATCGGCTTTA TTGCTCAAAG AGACATGGGT GGAAGAGATG
            661 AAGGTTACGA TTGGTTGATT ATGACACCCG GTGTGGGTTT AGATGACAAG GGAGACGCAT
            721 TGGGTCAACA GTATAGAACC GTGGATGATG TGGTCTCTAC AGGNTCTGAC ATTATTATTG
            781 TTGGAAGANG ANTATTTGCA AAGGNNANGG GATGCTAANG TAGAGGGTGA NNGTTACAGA
            841 AAAGCANGCT NGGAANCATA NTTGANAANA NGNNGNCNGC AAANNTNAAA AANNTGTATT
          901 ATAAGNNANT GCNNNNNNNC NNANNNNNCN ANNTNNNNCT NNNNNTNNNN NNNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (3xFLAG-GFP11, Pex3, GPD pro)
          Sequence file: RDB16052_A8I3i.seq check
          >D05803_B7_A8I3_2_CYC1ter_R_H02_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT
             61 AATTACATGA CTCGNGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TATGTTATCC
            121 CTGCAGCATT GACGTATTCA TGCAAAACCA TGTGATCTCT GGAAGTTGAA CCTCCACCAC
            181 TTCCGCCTTT ATCGTCATCA TCCTTATAGT CAATGTCATG ATCCTTATAA TCGCCATCAT
            241 GGTCTTTGTA ATCACCACCG GCAGAGGATC CGATCTGTTC TTTGATGAAG TGTTGCTCAG
            301 TAATCCGTAA CTGCTGTTTA TACAACCATC TCTTCACGAA AAACACTACC ACCGACCCTG
            361 TGGTAAATAA AGCAGCTATT CCAGTCAATG AAATGAGTAC CTTTCCTCGA TGTCTCTGCA
            421 GAAGCGAACG TGATCTTTGA TTTGGGGCCA TGCGGCCGCA TCCGTCGAAA CTAAGTTCTG
            481 GTGTTTTAAA ACTAAAAAAA AGACTAACTA TAAAAGTAGA ATTTAAGAAG TTTAAGAAAT
            541 AGATTTACAG AATTACAATC AATACCTACC GTCTTTATAT ACTTATTAGT CAAGTAGGGG
            601 AATAATTTCA GGGAACTGGT TTCAACCTTT TTTTTCAGCT TTTTCCAAAT CAGAGAGAGC
            661 AGAAGGTAAT AGAAGGTGTA AGAAAATGAG ATAGATACAT GCGTGGGTCA ATTGCCTTGT
            721 GTCATCATTT ACTCCAGGCA NGTTGCATCA CTCNATTGAN GTTGTGCCCG TTTTTTGCCT
            781 GTTTGTGCCC CNGTTCTCTG TANTTGCGCT NAGANAATGG ACCTATNAAC TGATGGTTGG
            841 TGAAGAAAAC AATATTTTNG GNGCTGGGNN TNTTTTTTTT TTTCTGGATN NNNGCTNNAA
          901 AANNCGGNNN NCATTATATT NNNNNGNNNN NNNNNN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB16052_A8I3j.seq check
          >D05803_B7_A8I3_2_Amp_R_H06_24_ABI24.ab1
              1 NNNNNNNNNN NNNTNNNNCA NTTATNNGGG TTATTGTCTC ATGAGCGGAN ACATATTTGA
             61 ATGTATTTAN AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
            121 TGGGTCCTTT TCATCACGTG CTATAAAAAT AATTATAATT TAAATTTTTT AATATAAATA
            181 TATAAATTAA AAATAGAAAG TAAAAAAAGA AATTAAAGAA AAAATAGTTT TTGTTTTCCG
            241 AAGATGTAAA AGACTCTAGG GGGATCGCCA ACAAATACTA CCTTTTATCT TGCTCTTCCT
            301 GCTCTCAGGT ATTAATGCCG AATTGTTTCA TCTTGTCTGT GTAGAAGACC ACACACGAAA
            361 ATCCTGTGAT TTTACATTTT ACTTATCGTT AATCGAATGT ATATCTATTT AATCTGCTTT
            421 TCTTGTCTAA TAAATATATA TGTAAAGTAC GCTTTTTGTT GAAATTTTTT AAACCTTTGT
            481 TTATTTTTTT TTCTTCATTC CGTAACTCTT CTACCTTCTT TATTTACTTT CTAAAATCCA
            541 AATACAAAAC ATAAAAATAA ATAAACACAG AGTAAATTCC CAAATTATTC CATCATTAAA
            601 AGATACGAGG CGCGTGTAAG TTACAGGCAA GCGATCCGTC TAAGAAACCA TTATTATCAT
            661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA
            721 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGANANG GTCACAGCTT GTCTGTAAGC
            781 GGATGCCGGG ANCAGACAAG CCCGTCANGG CGCGTCAGCG NGTGTTGGCG GGNGTCNGGG
            841 CTGGCTTAAC TATGCGGCAT CAGANCANAN TGTACTNNNA GNNCACCATA CCNCCTTTTC
            901 NNNNCATCAT TTTTTTTTTN ANNCNTTTTT TTTGANTNNN NNTNNNNNNN NANNTTTTTT
          961 NNNNNTNNNG NNNNNNNNCN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl