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Pex3N-FLAG-GFP11

Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 for visualizing organelle contact sites in vivo.

Catalog number RDB16052
Resource name Pex3N-FLAG-GFP11
Alternative name pRS316-GPDp-Pex3N-3xFLAG-GFP11 (pSFL96)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae PEX3 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae PEX3 (KEGG orthology K13336) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB16052 Pex3N-FLAG-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16052_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB16052_A8I3a.seq check
>D05803_B7_A8I3_2_M13_-40_E06_15_ABI24.ab1
    1 AGCACGCATG TATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC ATTATCAATA
   61 CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA CTTTATTTAG
  121 TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA GGGGGCGGGT
  181 TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG GCATCCACTA
  241 AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC CCAGCACCAA
  301 AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG CAACTACAGA
  361 GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT GCAACCTGCC
  421 TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA TTTTCTTACA
  481 CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG GTTGAAACCA
  541 GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA GGTATTGATT
  601 GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT TAGTCTTTTT
  661 TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCATGGC CCCAAATCAA
  721 AGATCACGTT CGCTTCTGCA GAGACATCGA GGAAAGGTAC TCATTTCATT GACTGGAATA
  781 GCTGCTTTAT TTACCACAGG GTCGGTGGTA GTGTTTTTTC GTGAAGAGAT GGTTGTATAA
  841 ACAGCAGTTA CGGATTACTG AGCAACACTT CATCAAAGAA CAGATCGGAT CCTCTGCCGG
  901 TGGTGATTAC AAAGACCATG ATGGCGATTA TAAGGATCAT GACATTGACT ATAAGGATGA
  961 TGACGATAAA GGCGGAAGGT GGTGGAGGTT TCAACTTCCC AGAGATCACA TGGTTTGCAT
 1021 GAGTACGTCA ATTGCTGGCA GGGATACCAT AAGAAGTCGA TATCAGGCTT AATCGATACC
1081 GATCGAACCC TTCG
//
Primer: CYC1ter_R
Sequence file: RDB16052_A8I3b.seq check
>D05803_B7_A8I3_2_CYC1ter_R_H02_23_ABI24.ab1
    1 CTTTCGTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TATGTTATCC
  121 CTGCAGCATT GACGTATTCA TGCAAAACCA TGTGATCTCT GGAAGTTGAA CCTCCACCAC
  181 TTCCGCCTTT ATCGTCATCA TCCTTATAGT CAATGTCATG ATCCTTATAA TCGCCATCAT
  241 GGTCTTTGTA ATCACCACCG GCAGAGGATC CGATCTGTTC TTTGATGAAG TGTTGCTCAG
  301 TAATCCGTAA CTGCTGTTTA TACAACCATC TCTTCACGAA AAACACTACC ACCGACCCTG
  361 TGGTAAATAA AGCAGCTATT CCAGTCAATG AAATGAGTAC CTTTCCTCGA TGTCTCTGCA
  421 GAAGCGAACG TGATCTTTGA TTTGGGGCCA TGCGGCCGCA TCCGTCGAAA CTAAGTTCTG
  481 GTGTTTTAAA ACTAAAAAAA AGACTAACTA TAAAAGTAGA ATTTAAGAAG TTTAAGAAAT
  541 AGATTTACAG AATTACAATC AATACCTACC GTCTTTATAT ACTTATTAGT CAAGTAGGGG
  601 AATAATTTCA GGGAACTGGT TTCAACCTTT TTTTTCAGCT TTTTCCAAAT CAGAGAGAGC
  661 AGAAGGTAAT AGAAGGTGTA AGAAAATGAG ATAGATACAT GCGTGGGTCA ATTGCCTTGT
  721 GTCATCATTT ACTCCAGGCA GGTTGCATCA CTCCATTGAG GTTGTGCCCG TTTTTTGCCT
  781 GTTTGTGCCC CTGTTCTCTG TAGTTGCGCT AAGAGAATGG ACCTATGAAC TGATGGTTGG
  841 TGAAGAAAAC AATATTTTTG GTGCTGGGAT TCTTTTTTTT TTTCTGGATG CCAGCTTAAA
  901 AAAGCGGGCT CCATTATATT TAGTGGGATG CCAGGAATAA ACTGTTCACC CAGACACCCT
  961 ACGATGTTAT ATATTCCTGT GTAACCCGCC CCCCTATTTT TGGGGCATGT TACTGGCTAC
 1021 AGCAGCATTT AAAAGCCTTA TTTTTTGGAA CTAATCAAGT AGAAATCACT ACTATTTAAT
//
Primer: URA3pro_F
Sequence file: RDB16052_A8I3c.seq check
>D05803_B7_A8I3_2_URA3pro_F_G06_21_ABI24.ab1
    1 CAATCTAGTG TTGAGACATG AATTGCCCAG TATTCTTAAC CCAACTGCAC AGAACAAAAA
   61 CCTGCAGGAA ACGAAGATAA ATCATGTCGA AAGCTACATA TAAGGAACGT GCTGCTACTC
  121 ATCCTAGTCC TGTTGCTGCC AAGCTATTTA ATATCATGCA CGAAAAGCAA ACAAACTTGT
  181 GTGCTTCATT GGATGTTCGT ACCACCAAGG AATTACTGGA GTTAGTTGAA GCATTAGGTC
  241 CCAAAATTTG TTTACTAAAA ACACATGTGG ATATCTTGAC TGATTTTTCC ATGGAGGGCA
  301 CAGTTAAGCC GCTAAAGGCA TTATCCGCCA AGTACAATTT TTTACTCTTC GAAGACAGAA
  361 AATTTGCTGA CATTGGTAAT ACAGTCAAAT TGCAGTACTC TGCGGGTGTA TACAGAATAG
  421 CAGAATGGGC AGACATTACG AATGCACACG GTGTGGTGGG CCCAGGTATT GTTAGCGGTT
  481 TGAAGCAGGC GGCAGAAGAA GTAACAAAGG AACCTAGAGG CCTTTTGATG TTAGCAGAAT
  541 TGTCATGCAA GGGCTCCCTA TCTACTGGAG AATATACTAA GGGTACTGTT GACATTGCGA
  601 AGAGCGACAA AGATTTTGTT ATCGGCTTTA TTGCTCAAAG AGACATGGGT GGAAGAGATG
  661 AAGGTTACGA TTGGTTGATT ATGACACCCG GTGTGGGTTT AGATGACAAG GGAGACGCAT
  721 TGGGTCAACA GTATAGAACC GTGGATGATG TGGTCTCTAC AGGATCTGAC ATTATTATTG
  781 TTGGAAGAGG ACTATTTGCA AAGGGAAGGG GATGCTAAGG TAGAGGGTGA ACGTTACAGA
  841 AAAGCAGGCT GGGAAGCATA TTTGAGAAGA TGCGGCCAGC AAAACTAAAA AAACTGTATT
  901 ATAAGTAAAT GCATGTATAC TAAACTCACA AATTAGAGCT TCAATTTAAT TATATCAGTT
  961 TATTACCCTA TGCGGGTGTG AAAATACCCG CACAGATGCG TAAAGGAGAA AAATACGGCA
 1021 TCATGAAATT TGTAAGCCGA TCATATTTTG TAAAAATTCG CGATAATTTT TGGTAAATTC
1081 AGCTCATTTT TTTTAAC
//
Primer: Amp_R
Sequence file: RDB16052_A8I3d.seq check
>D05803_B7_A8I3_2_Amp_R_H06_24_ABI24.ab1
    1 TTATTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
   61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
  121 TGGGTCCTTT TCATCACGTG CTATAAAAAT AATTATAATT TAAATTTTTT AATATAAATA
  181 TATAAATTAA AAATAGAAAG TAAAAAAAGA AATTAAAGAA AAAATAGTTT TTGTTTTCCG
  241 AAGATGTAAA AGACTCTAGG GGGATCGCCA ACAAATACTA CCTTTTATCT TGCTCTTCCT
  301 GCTCTCAGGT ATTAATGCCG AATTGTTTCA TCTTGTCTGT GTAGAAGACC ACACACGAAA
  361 ATCCTGTGAT TTTACATTTT ACTTATCGTT AATCGAATGT ATATCTATTT AATCTGCTTT
  421 TCTTGTCTAA TAAATATATA TGTAAAGTAC GCTTTTTGTT GAAATTTTTT AAACCTTTGT
  481 TTATTTTTTT TTCTTCATTC CGTAACTCTT CTACCTTCTT TATTTACTTT CTAAAATCCA
  541 AATACAAAAC ATAAAAATAA ATAAACACAG AGTAAATTCC CAAATTATTC CATCATTAAA
  601 AGATACGAGG CGCGTGTAAG TTACAGGCAA GCGATCCGTC TAAGAAACCA TTATTATCAT
  661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA
  721 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC
  781 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG
  841 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA CCACCTTTTC
  901 AATTCATCAT TTTTTTTTTT ATTCTTTTTT TTTGATTTCG GTTTCCCTTG AAATTTTTTT
  961 TTGATTTCGG GTAATCTCCC GACAGAAGGA GAACGACGAT GAGCACAGGA CTTAGATTGG
 1021 TATATATACG CATATGTAGT GATGAAGAAA CATGGAGTGT CCAGTATCTA ACTCGAACTG
1081 CACAGGAACC AAATCCTGGC G
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl