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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Pex3N-FLAG-GFP11 (#RDB16052)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: 3xFLAG-GFP11; location: peroxisome.

Alternative name pSFL96, pRS316-GPDp-Pex3N-3xFLAG-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae PEX3 cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB16052zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

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RDB16052 Pex3N-FLAG-GFP11 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Pex3N-FLAG-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16052).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16052_A8I3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800)
Region: T7 pro, insert 5' (GDP pro)
Sequence file: RDB16052_A8I3f.seq check
>16052_16052_B0KqF_HB-NOS_R_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNNNNNNGGT ANGCCNGGGT TTTCCCNGTC ACGACGTTGT
   61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA
  121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT
  181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA
  241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC
  301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA
  361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG
  421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG
  481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT
  541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA
  601 AGGTTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG
  661 TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA
  721 GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCATG
  781 GCCNCAAATC AAAGATCACG TTCGCTTCTG CAGANACATC GAGGAAAGGT ACTCATTTCA
  841 TTGACTGGAA TANCTGCTTT ATTTACCACA NGGNCNGNGG TANNGNTTTT TCGNGAANAG
  901 ATGGTTGNAT NAACAGCANT TACNGNNTAC TGANCNACNC TTCATNNNNN NNANNNCNNN
961 NNNNCTGNNN GNGNNNANNN NNANNANNNN NNNNNN
//
Primer: SV40pA3'element Reverse (Pr0108)
Region: T3 pro, insert 3' (CYC1 ter, 3xFLAG-GFP11 3')
Sequence file: RDB16052_A8I3g.seq check
>D05803_B7_A8I3_2_SV40pA3'element Reverse_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNNNNNCNNG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
   61 TTGCTGNCCT TTTGCTNNCA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
  121 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
  181 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
  241 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
  301 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
  361 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA
  421 TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGTACC
  481 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA
  541 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG
  601 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA
  661 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA
  721 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTATGTTAT
  781 CCCTGCAGCA TTGACGTATT CATGCAAAAC CATGTGATCT CTGGAANTTN NACCNCCACC
  841 ACTTCCGCCT TTATCGNCAT CATCCTTATA GNCAATGNCA NGNTCCTTAT NANCGCCATC
901 ANNGNCTTNN NNNTCACNAC NGGNANNNNN NNNNN
//
Primer: URA3pro_F (Pr0170)
Region: URA3 pro, URA3
Sequence file: RDB16052_A8I3h.seq check
>D05803_B7_A8I3_2_URA3pro_F_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTG ANNTGCCNNG TATTCTTAAC CCAACTGCAC AGAACAAAAA
   61 CCTGCAGGNN NNGAAGANAA ATCATGTCGA AAGCTACATA TAAGGAACGT GCTGCTACTC
  121 ATCCTAGTCC TGTTGCTGCC AAGCTATTTA ATATCATGCA CGAAAAGCAA ACAAACTTGT
  181 GTGCTTCATT GGATGTTCGT ACCACCAAGG AATTACTGGA GTTAGTTGAA GCATTAGGTC
  241 CCAAAATTTG TTTACTAAAA ACACATGTGG ATATCTTGAC TGATTTTTCC ATGGAGGGCA
  301 CAGTTAAGCC GCTAAAGGCA TTATCCGCCA AGTACAATTT TTTACTCTTC GAAGACAGAA
  361 AATTTGCTGA CATTGGTAAT ACAGTCAAAT TGCAGTACTC TGCGGGTGTA TACAGAATAG
  421 CAGAATGGGC AGACATTACG AATGCACACG GTGTGGTGGG CCCAGGTATT GTTAGCGGTT
  481 TGAAGCAGGC GGCAGAAGAA GTAACAAAGG AACCTAGAGG CCTTTTGATG TTAGCAGAAT
  541 TGTCATGCAA GGGCTCCCTA TCTACTGGAG AATATACTAA GGGTACTGTT GACATTGCGA
  601 AGAGCGACAA AGATTTTGTT ATCGGCTTTA TTGCTCAAAG AGACATGGGT GGAAGAGATG
  661 AAGGTTACGA TTGGTTGATT ATGACACCCG GTGTGGGTTT AGATGACAAG GGAGACGCAT
  721 TGGGTCAACA GTATAGAACC GTGGATGATG TGGTCTCTAC AGGNTCTGAC ATTATTATTG
  781 TTGGAAGANG ANTATTTGCA AAGGNNANGG GATGCTAANG TAGAGGGTGA NNGTTACAGA
  841 AAAGCANGCT NGGAANCATA NTTGANAANA NGNNGNCNGC AAANNTNAAA AANNTGTATT
901 ATAAGNNANT GCNNNNNNNC NNANNNNNCN ANNTNNNNCT NNNNNTNNNN NNNNNN
//
Primer: CYC1ter_R (Pr0022)
Region: insert mid (3xFLAG-GFP11, Pex3, GPD pro)
Sequence file: RDB16052_A8I3i.seq check
>D05803_B7_A8I3_2_CYC1ter_R_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGNGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TATGTTATCC
  121 CTGCAGCATT GACGTATTCA TGCAAAACCA TGTGATCTCT GGAAGTTGAA CCTCCACCAC
  181 TTCCGCCTTT ATCGTCATCA TCCTTATAGT CAATGTCATG ATCCTTATAA TCGCCATCAT
  241 GGTCTTTGTA ATCACCACCG GCAGAGGATC CGATCTGTTC TTTGATGAAG TGTTGCTCAG
  301 TAATCCGTAA CTGCTGTTTA TACAACCATC TCTTCACGAA AAACACTACC ACCGACCCTG
  361 TGGTAAATAA AGCAGCTATT CCAGTCAATG AAATGAGTAC CTTTCCTCGA TGTCTCTGCA
  421 GAAGCGAACG TGATCTTTGA TTTGGGGCCA TGCGGCCGCA TCCGTCGAAA CTAAGTTCTG
  481 GTGTTTTAAA ACTAAAAAAA AGACTAACTA TAAAAGTAGA ATTTAAGAAG TTTAAGAAAT
  541 AGATTTACAG AATTACAATC AATACCTACC GTCTTTATAT ACTTATTAGT CAAGTAGGGG
  601 AATAATTTCA GGGAACTGGT TTCAACCTTT TTTTTCAGCT TTTTCCAAAT CAGAGAGAGC
  661 AGAAGGTAAT AGAAGGTGTA AGAAAATGAG ATAGATACAT GCGTGGGTCA ATTGCCTTGT
  721 GTCATCATTT ACTCCAGGCA NGTTGCATCA CTCNATTGAN GTTGTGCCCG TTTTTTGCCT
  781 GTTTGTGCCC CNGTTCTCTG TANTTGCGCT NAGANAATGG ACCTATNAAC TGATGGTTGG
  841 TGAAGAAAAC AATATTTTNG GNGCTGGGNN TNTTTTTTTT TTTCTGGATN NNNGCTNNAA
901 AANNCGGNNN NCATTATATT NNNNNGNNNN NNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro, CEN_ARS
Sequence file: RDB16052_A8I3j.seq check
>D05803_B7_A8I3_2_Amp_R_H06_24_ABI24.ab1
    1 NNNNNNNNNN NNNTNNNNCA NTTATNNGGG TTATTGTCTC ATGAGCGGAN ACATATTTGA
   61 ATGTATTTAN AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
  121 TGGGTCCTTT TCATCACGTG CTATAAAAAT AATTATAATT TAAATTTTTT AATATAAATA
  181 TATAAATTAA AAATAGAAAG TAAAAAAAGA AATTAAAGAA AAAATAGTTT TTGTTTTCCG
  241 AAGATGTAAA AGACTCTAGG GGGATCGCCA ACAAATACTA CCTTTTATCT TGCTCTTCCT
  301 GCTCTCAGGT ATTAATGCCG AATTGTTTCA TCTTGTCTGT GTAGAAGACC ACACACGAAA
  361 ATCCTGTGAT TTTACATTTT ACTTATCGTT AATCGAATGT ATATCTATTT AATCTGCTTT
  421 TCTTGTCTAA TAAATATATA TGTAAAGTAC GCTTTTTGTT GAAATTTTTT AAACCTTTGT
  481 TTATTTTTTT TTCTTCATTC CGTAACTCTT CTACCTTCTT TATTTACTTT CTAAAATCCA
  541 AATACAAAAC ATAAAAATAA ATAAACACAG AGTAAATTCC CAAATTATTC CATCATTAAA
  601 AGATACGAGG CGCGTGTAAG TTACAGGCAA GCGATCCGTC TAAGAAACCA TTATTATCAT
  661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA
  721 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGANANG GTCACAGCTT GTCTGTAAGC
  781 GGATGCCGGG ANCAGACAAG CCCGTCANGG CGCGTCAGCG NGTGTTGGCG GGNGTCNGGG
  841 CTGGCTTAAC TATGCGGCAT CAGANCANAN TGTACTNNNA GNNCACCATA CCNCCTTTTC
  901 NNNNCATCAT TTTTTTTTTN ANNCNTTTTT TTTGANTNNN NNTNNNNNNN NANNTTTTTT
961 NNNNNTNNNG NNNNNNNNCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]