The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: 3xFLAG-GFP11; location: peroxisome.
Alternative name | pSFL96, pRS316-GPDp-Pex3N-3xFLAG-GFP11 |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS316 (plasmid) |
Selectable markers | Amp^r (E. coli), URA3 |
Gene/insert name | S. cerevisiae PEX3 cDNA |
Depositor|Developer | Tamura, Yasushi | |
Sequence (full) ![]() |
RDB16052zzt01.seq compiled by us |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16052 | Pex3N-FLAG-GFP11 | DNA solution |
Materials & Methods section:
The Pex3N-FLAG-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16052). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16052_A8I3p1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GDP pro) Sequence file: RDB16052_A8I3f.seq ![]() |
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>16052_16052_B0KqF_HB-NOS_R_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNN NNNNNNNGGT ANGCCNGGGT TTTCCCNGTC ACGACGTTGT 61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA 121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT 181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA 241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC 301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA 361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG 421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG 481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT 541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA 601 AGGTTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG 661 TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA 721 GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCATG 781 GCCNCAAATC AAAGATCACG TTCGCTTCTG CAGANACATC GAGGAAAGGT ACTCATTTCA 841 TTGACTGGAA TANCTGCTTT ATTTACCACA NGGNCNGNGG TANNGNTTTT TCGNGAANAG 901 ATGGTTGNAT NAACAGCANT TACNGNNTAC TGANCNACNC TTCATNNNNN NNANNNCNNN 961 NNNNCTGNNN GNGNNNANNN NNANNANNNN NNNNNN // |
Primer: SV40pA3'element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, 3xFLAG-GFP11 3') Sequence file: RDB16052_A8I3g.seq ![]() |
>D05803_B7_A8I3_2_SV40pA3'element Reverse_F06_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG GNNNNNCNNG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 61 TTGCTGNCCT TTTGCTNNCA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 121 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 181 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 241 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG 301 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT 361 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA 421 TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGTACC 481 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA 541 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG 601 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA 661 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA 721 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTATGTTAT 781 CCCTGCAGCA TTGACGTATT CATGCAAAAC CATGTGATCT CTGGAANTTN NACCNCCACC 841 ACTTCCGCCT TTATCGNCAT CATCCTTATA GNCAATGNCA NGNTCCTTAT NANCGCCATC 901 ANNGNCTTNN NNNTCACNAC NGGNANNNNN NNNNN // |
Primer: URA3pro_F (Pr0170) Region: URA3 pro, URA3 Sequence file: RDB16052_A8I3h.seq ![]() |
>D05803_B7_A8I3_2_URA3pro_F_G06_21_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTG ANNTGCCNNG TATTCTTAAC CCAACTGCAC AGAACAAAAA 61 CCTGCAGGNN NNGAAGANAA ATCATGTCGA AAGCTACATA TAAGGAACGT GCTGCTACTC 121 ATCCTAGTCC TGTTGCTGCC AAGCTATTTA ATATCATGCA CGAAAAGCAA ACAAACTTGT 181 GTGCTTCATT GGATGTTCGT ACCACCAAGG AATTACTGGA GTTAGTTGAA GCATTAGGTC 241 CCAAAATTTG TTTACTAAAA ACACATGTGG ATATCTTGAC TGATTTTTCC ATGGAGGGCA 301 CAGTTAAGCC GCTAAAGGCA TTATCCGCCA AGTACAATTT TTTACTCTTC GAAGACAGAA 361 AATTTGCTGA CATTGGTAAT ACAGTCAAAT TGCAGTACTC TGCGGGTGTA TACAGAATAG 421 CAGAATGGGC AGACATTACG AATGCACACG GTGTGGTGGG CCCAGGTATT GTTAGCGGTT 481 TGAAGCAGGC GGCAGAAGAA GTAACAAAGG AACCTAGAGG CCTTTTGATG TTAGCAGAAT 541 TGTCATGCAA GGGCTCCCTA TCTACTGGAG AATATACTAA GGGTACTGTT GACATTGCGA 601 AGAGCGACAA AGATTTTGTT ATCGGCTTTA TTGCTCAAAG AGACATGGGT GGAAGAGATG 661 AAGGTTACGA TTGGTTGATT ATGACACCCG GTGTGGGTTT AGATGACAAG GGAGACGCAT 721 TGGGTCAACA GTATAGAACC GTGGATGATG TGGTCTCTAC AGGNTCTGAC ATTATTATTG 781 TTGGAAGANG ANTATTTGCA AAGGNNANGG GATGCTAANG TAGAGGGTGA NNGTTACAGA 841 AAAGCANGCT NGGAANCATA NTTGANAANA NGNNGNCNGC AAANNTNAAA AANNTGTATT 901 ATAAGNNANT GCNNNNNNNC NNANNNNNCN ANNTNNNNCT NNNNNTNNNN NNNNNN // |
Primer: CYC1ter_R (Pr0022) Region: insert mid (3xFLAG-GFP11, Pex3, GPD pro) Sequence file: RDB16052_A8I3i.seq ![]() |
>D05803_B7_A8I3_2_CYC1ter_R_H02_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT 61 AATTACATGA CTCGNGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TATGTTATCC 121 CTGCAGCATT GACGTATTCA TGCAAAACCA TGTGATCTCT GGAAGTTGAA CCTCCACCAC 181 TTCCGCCTTT ATCGTCATCA TCCTTATAGT CAATGTCATG ATCCTTATAA TCGCCATCAT 241 GGTCTTTGTA ATCACCACCG GCAGAGGATC CGATCTGTTC TTTGATGAAG TGTTGCTCAG 301 TAATCCGTAA CTGCTGTTTA TACAACCATC TCTTCACGAA AAACACTACC ACCGACCCTG 361 TGGTAAATAA AGCAGCTATT CCAGTCAATG AAATGAGTAC CTTTCCTCGA TGTCTCTGCA 421 GAAGCGAACG TGATCTTTGA TTTGGGGCCA TGCGGCCGCA TCCGTCGAAA CTAAGTTCTG 481 GTGTTTTAAA ACTAAAAAAA AGACTAACTA TAAAAGTAGA ATTTAAGAAG TTTAAGAAAT 541 AGATTTACAG AATTACAATC AATACCTACC GTCTTTATAT ACTTATTAGT CAAGTAGGGG 601 AATAATTTCA GGGAACTGGT TTCAACCTTT TTTTTCAGCT TTTTCCAAAT CAGAGAGAGC 661 AGAAGGTAAT AGAAGGTGTA AGAAAATGAG ATAGATACAT GCGTGGGTCA ATTGCCTTGT 721 GTCATCATTT ACTCCAGGCA NGTTGCATCA CTCNATTGAN GTTGTGCCCG TTTTTTGCCT 781 GTTTGTGCCC CNGTTCTCTG TANTTGCGCT NAGANAATGG ACCTATNAAC TGATGGTTGG 841 TGAAGAAAAC AATATTTTNG GNGCTGGGNN TNTTTTTTTT TTTCTGGATN NNNGCTNNAA 901 AANNCGGNNN NCATTATATT NNNNNGNNNN NNNNNN // |
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB16052_A8I3j.seq ![]() |
>D05803_B7_A8I3_2_Amp_R_H06_24_ABI24.ab1 1 NNNNNNNNNN NNNTNNNNCA NTTATNNGGG TTATTGTCTC ATGAGCGGAN ACATATTTGA 61 ATGTATTTAN AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 121 TGGGTCCTTT TCATCACGTG CTATAAAAAT AATTATAATT TAAATTTTTT AATATAAATA 181 TATAAATTAA AAATAGAAAG TAAAAAAAGA AATTAAAGAA AAAATAGTTT TTGTTTTCCG 241 AAGATGTAAA AGACTCTAGG GGGATCGCCA ACAAATACTA CCTTTTATCT TGCTCTTCCT 301 GCTCTCAGGT ATTAATGCCG AATTGTTTCA TCTTGTCTGT GTAGAAGACC ACACACGAAA 361 ATCCTGTGAT TTTACATTTT ACTTATCGTT AATCGAATGT ATATCTATTT AATCTGCTTT 421 TCTTGTCTAA TAAATATATA TGTAAAGTAC GCTTTTTGTT GAAATTTTTT AAACCTTTGT 481 TTATTTTTTT TTCTTCATTC CGTAACTCTT CTACCTTCTT TATTTACTTT CTAAAATCCA 541 AATACAAAAC ATAAAAATAA ATAAACACAG AGTAAATTCC CAAATTATTC CATCATTAAA 601 AGATACGAGG CGCGTGTAAG TTACAGGCAA GCGATCCGTC TAAGAAACCA TTATTATCAT 661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA 721 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGANANG GTCACAGCTT GTCTGTAAGC 781 GGATGCCGGG ANCAGACAAG CCCGTCANGG CGCGTCAGCG NGTGTTGGCG GGNGTCNGGG 841 CTGGCTTAAC TATGCGGCAT CAGANCANAN TGTACTNNNA GNNCACCATA CCNCCTTTTC 901 NNNNCATCAT TTTTTTTTTN ANNCNTTTTT TTTGANTNNN NNTNNNNNNN NANNTTTTTT 961 NNNNNTNNNG NNNNNNNNCN NNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
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