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Pex3N-GFP1-10

Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 for visualizing organelle contact sites in vivo.

Catalog number RDB16051
Resource name Pex3N-GFP1-10
Alternative name pRS316-GPDp-Pex3N-GFP1-10 (pSFL26)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae PEX3 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae PEX3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16051 Pex3N-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16051zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16051_A8I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro)
          Sequence file: RDB16051_A8I3e.seq check
          >
          //
          Primer: SV40pA3'element Reverse
          Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
          Sequence file: RDB16051_A8I3f.seq check
          >D05803_B6_A8I3_1_SV40pA3'element Reverse_B06_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG GNNNNNCCNG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
             61 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
            121 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
            181 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
            241 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
            301 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
            361 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA
            421 TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGTACC
            481 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA
            541 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG
            601 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA
            661 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA
            721 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTACTTTTC
            781 GTTGGGATCT TTCGAAAGGA NAGTTTGTGT CGACAGGNAA TGGTTGTCTG GNAAAANGAN
            841 AGGGCCATCG CCNATNNGAN NANNNNGTNN ATAANNGNCT GCTANTTNNN NGGAANCNTC
          901 TTCNNNNTNN NNNNNNNN
          //
          Primer: paGFP-M_R1
          Region: insert mid (GFP1-10 5', Pex3N, GPD pro)
          Sequence file: RDB16051_A8I3g.seq check
          >16051_16051_B0KqF_paGFP-M_R1_C09_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTGC CTTTTNNTGT GNNCCGGANA ACGGGAAAAG CATTGAACAC
             61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA
            121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA
            181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA
            241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC
            301 CGATCTGTTC TTTGATGAAG TGTTGCTCAG TAATCCGTAA CTGCTGTTTA TACAACCATC
            361 TCTTCACGAA AAACACTACC ACCGACCCTG TGGTAAATAA AGCAGCTATT CCAGTCAATG
            421 AAATGAGTAC CTTTCCTCGA TGTCTCTGCA GAAGCGAACG TGATCTTTGA TTTGGGGCCA
            481 TGCGGCCGCA TCCGTCGAAA CTAAGTTCTG GTGTTTTAAA ACTAAAAAAA AGACTAACTA
            541 TAAAAGTAGA ATTTAAGAAG TTTAAGAAAT AGATTTACAG AATTACAATC AATACCTACC
            601 GTCTTTATAT ACTTATTAGT CAAGTAGGGG AATAATTTCA GGGAACTGGT TTCAACCTTT
            661 TTTTTCAGCT TTTTCCAAAT CAGAGAGAGC AGAAGGTAAT AGAAGGTGTA AGAAAATGAG
            721 ATAGATACAT GCGTGGGTCA ATTGCCTTGT GTCATCATTT ACTCCAGGCA GGTTGCATCA
            781 CTCCATTGAN GTTGTGCCCG TTTTTTGCCT GTTTGTGCCC CNGTTCTCTG TANTTGCGCT
            841 AAGAGAATGG ANCTATGAAC TGATGGTTGG TGAANAAAAC AATATTTTGG TGNTGGGATT
            901 CTTTTTTTTT TTCNGGNTGN CANCTTAAAA AAGCGGNNTN NATTATANTT NAGTGNANGN
          961 CANGNANTAA ANNNNTCNNC CNNANNNCNN NNNNNNNN
          //
          Primer: URA3pro_F
          Region: URA3pro, URA3
          Sequence file: RDB16051_A8I3h.seq check
          >D05803_B6_A8I3_1_URA3pro_F_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TNNNNNGCCN NGTATTCTTA ACCCNACTGC ACAGAACAAA
             61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
            121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
            181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
            241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
            301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
            361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
            421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
            481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
            541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
            601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
            661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
            721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
            781 TGTTGGAAGA NGANTATTTG CAAAGGGAAN GGATGCTAAN GTAGAGGGTG AACGTTACAG
            841 AAAAGCANGC TGGGAANCAT ATTTGAGAAN ANGCNGNNNN CAAANCTAAA AAACTGTATT
            901 ATAAGTNANT GCANNNATAC TAAANNNNCN ANTTANNNCT TNNNTTTNAT TANATCANNN
          961 NNNNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP1-10 3')
          Sequence file: RDB16051_A8I3i.seq check
          >D05803_B6_A8I3_1_CYC1ter_R_G02_20_ABI24.ab1
              1 NNNNNNNNNN NNGNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT
             61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
            121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
            181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
            241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
            301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
            361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
            421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
            481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
            541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
            601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
            661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
            721 CAACTCCAGT GANNNGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
            781 CAGACCCTCC GGNTNCGATC TGTNCTTTGA TGAAGTGNTG CTCAGTAATC CGTAACTGCT
            841 GTTTATACNA CCATCTCTTC ACGAAAAANN NCTACNACNN ANCNNNNNGN AAANNANNNA
          901 NCNNNNNNNN NN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB16051_A8I3j.seq check
          >D05803_B6_A8I3_1_Amp_R_D06_12_ABI24.ab1
              1 NNNNNNNNNN NNATTNNNNC NNTNATNNGG GTTATTGTCT CNTGAGCGGA NACATATTTG
             61 AATGTATNNN NNNNAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
            181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
            241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
            301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
            361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
            421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
            481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
            541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
            601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
            661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
            721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGANAC GGTCACAGCT TGTCTGTAAG
            781 CGGATGCCGG GAGCAGANAA GCCCGTCANG GCGCGTCAGC GGGNGTTGGC GGGTGTCGGG
            841 GCTGGCTTAA CTATGCGGCA TCAGANCANA NTGTACTNAG ANTGNACCAT ACCACCTTTT
            901 CAATTNATCN TTTTTTTTTT TNNNNNCNTT TTTTTNGANT TNNNNTTNCN NTNNNAANTT
          961 TTTTTNNNNT NCNNNNANNN NNNNNANNNN NANNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl