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Pex3N-GFP1-10

Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 for visualizing organelle contact sites in vivo.

Catalog number RDB16051
Resource name Pex3N-GFP1-10
Alternative name pRS316-GPDp-Pex3N-GFP1-10 (pSFL26)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae PEX3 cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae PEX3 (KEGG orthology K13336) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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RDB16051 Pex3N-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16051_A8I3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB16051_A8I3e.seq check
>16051_16051_B0KqF_HB-NOS_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GTNNNGCCNG GGTTTTCCCN GTCNCGACGT
   61 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCAGT
  121 TTATCATTAT CAATACTCGC CATTTCAAAG AATACGTAAA TAATTAATAG TAGTGATTTT
  181 CCTAACTTTA TTTAGTCAAA AAATTAGCCT TTTAATTCTG CTGTAACCCG TACATGCCCA
  241 AAATAGGGGG CGGGTTACAC AGAATATATA ACATCGTAGG TGTCTGGGTG AACAGTTTAT
  301 TCCTGGCATC CACTAAATAT AATGGAGCCC GCTTTTTAAG CTGGCATCCA GAAAAAAAAA
  361 GAATCCCAGC ACCAAAATAT TGTTTTCTTC ACCAACCATC AGTTCATAGG TCCATTCTCT
  421 TAGCGCAACT ACAGAGAACA GGGGCACAAA CAGGCAAAAA ACGGGCACAA CCTCAATGGA
  481 GTGATGCAAC CTGCCTGGAG TAAATGATGA CACAAGGCAA TTGACCCACG CATGTATCTA
  541 TCTCATTTTC TTACACCTTC TATTACCTTC TGCTCTCTCT GATTTGGAAA AAGCTGAAAA
  601 AAAAGGNTGA AACCAGTTCC CTGAAATTAT TCCCCTACTT GACTAATAAG TATATAAAGA
  661 CNGTNNNNAT TGATTGNAAT TCTGTAAATC TATTTCTTAA ACTTCTTAAA NTCTACTTTT
  721 ATAGTNAGTC NTTTTTTTTA NTTTTNAAAN NCNNNANNNN ANTTTCNNNN NNNGNNNNNN
781 NNNN
//
Primer: SV40pA3'element Reverse
Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
Sequence file: RDB16051_A8I3f.seq check
>D05803_B6_A8I3_1_SV40pA3'element Reverse_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNNNNNCCNG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
   61 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
  121 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
  181 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
  241 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
  301 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
  361 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA
  421 TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGTACC
  481 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA
  541 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG
  601 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA
  661 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA
  721 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTACTTTTC
  781 GTTGGGATCT TTCGAAAGGA NAGTTTGTGT CGACAGGNAA TGGTTGTCTG GNAAAANGAN
  841 AGGGCCATCG CCNATNNGAN NANNNNGTNN ATAANNGNCT GCTANTTNNN NGGAANCNTC
901 TTCNNNNTNN NNNNNNNN
//
Primer: paGFP-M_R1
Region: insert mid (GFP1-10 5', Pex3N, GPD pro)
Sequence file: RDB16051_A8I3g.seq check
>16051_16051_B0KqF_paGFP-M_R1_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTGC CTTTTNNTGT GNNCCGGANA ACGGGAAAAG CATTGAACAC
   61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA
  121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA
  181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA
  241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC
  301 CGATCTGTTC TTTGATGAAG TGTTGCTCAG TAATCCGTAA CTGCTGTTTA TACAACCATC
  361 TCTTCACGAA AAACACTACC ACCGACCCTG TGGTAAATAA AGCAGCTATT CCAGTCAATG
  421 AAATGAGTAC CTTTCCTCGA TGTCTCTGCA GAAGCGAACG TGATCTTTGA TTTGGGGCCA
  481 TGCGGCCGCA TCCGTCGAAA CTAAGTTCTG GTGTTTTAAA ACTAAAAAAA AGACTAACTA
  541 TAAAAGTAGA ATTTAAGAAG TTTAAGAAAT AGATTTACAG AATTACAATC AATACCTACC
  601 GTCTTTATAT ACTTATTAGT CAAGTAGGGG AATAATTTCA GGGAACTGGT TTCAACCTTT
  661 TTTTTCAGCT TTTTCCAAAT CAGAGAGAGC AGAAGGTAAT AGAAGGTGTA AGAAAATGAG
  721 ATAGATACAT GCGTGGGTCA ATTGCCTTGT GTCATCATTT ACTCCAGGCA GGTTGCATCA
  781 CTCCATTGAN GTTGTGCCCG TTTTTTGCCT GTTTGTGCCC CNGTTCTCTG TANTTGCGCT
  841 AAGAGAATGG ANCTATGAAC TGATGGTTGG TGAANAAAAC AATATTTTGG TGNTGGGATT
  901 CTTTTTTTTT TTCNGGNTGN CANCTTAAAA AAGCGGNNTN NATTATANTT NAGTGNANGN
961 CANGNANTAA ANNNNTCNNC CNNANNNCNN NNNNNNNN
//
Primer: URA3pro_F
Region: URA3pro, URA3
Sequence file: RDB16051_A8I3h.seq check
>D05803_B6_A8I3_1_URA3pro_F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TNNNNNGCCN NGTATTCTTA ACCCNACTGC ACAGAACAAA
   61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
  121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
  181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
  241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
  301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
  361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
  421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
  481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
  541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
  601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
  661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
  721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
  781 TGTTGGAAGA NGANTATTTG CAAAGGGAAN GGATGCTAAN GTAGAGGGTG AACGTTACAG
  841 AAAAGCANGC TGGGAANCAT ATTTGAGAAN ANGCNGNNNN CAAANCTAAA AAACTGTATT
  901 ATAAGTNANT GCANNNATAC TAAANNNNCN ANTTANNNCT TNNNTTTNAT TANATCANNN
961 NNNNNNN
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, GFP1-10 3')
Sequence file: RDB16051_A8I3i.seq check
>D05803_B6_A8I3_1_CYC1ter_R_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
  121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
  181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
  241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
  301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
  361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
  421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
  481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
  541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
  601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
  661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
  721 CAACTCCAGT GANNNGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
  781 CAGACCCTCC GGNTNCGATC TGTNCTTTGA TGAAGTGNTG CTCAGTAATC CGTAACTGCT
  841 GTTTATACNA CCATCTCTTC ACGAAAAANN NCTACNACNN ANCNNNNNGN AAANNANNNA
901 NCNNNNNNNN NN
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB16051_A8I3j.seq check
>D05803_B6_A8I3_1_Amp_R_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNATTNNNNC NNTNATNNGG GTTATTGTCT CNTGAGCGGA NACATATTTG
   61 AATGTATNNN NNNNAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
  181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
  241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
  301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
  361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
  421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
  481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
  541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
  601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGANAC GGTCACAGCT TGTCTGTAAG
  781 CGGATGCCGG GAGCAGANAA GCCCGTCANG GCGCGTCAGC GGGNGTTGGC GGGTGTCGGG
  841 GCTGGCTTAA CTATGCGGCA TCAGANCANA NTGTACTNAG ANTGNACCAT ACCACCTTTT
  901 CAATTNATCN TTTTTTTTTT TNNNNNCNTT TTTTTNGANT TNNNNTTNCN NTNNNAANTT
961 TTTTTNNNNT NCNNNNANNN NNNNNANNNN NANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2021.01.06

GNP_filter3_RDBDEP_html_201222.pl