The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: peroxisome.
Alternative name | pSFL26, pRS316-GPDp-Pex3N-GFP1-10 |
---|---|
Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS316 (plasmid) |
Selectable markers | Amp^r (E. coli), URA3 |
Gene/insert name | S. cerevisiae PEX3 cDNA |
Depositor|Developer | Tamura, Yasushi | |
Sequence (full) ![]() |
RDB16051zzt01.seq compiled by us |
---|
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
---|---|
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
---|---|
MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB16051 | Pex3N-GFP1-10 | DNA solution |
Materials & Methods section:
The Pex3N-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16051). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16051_A8I3p1-2.pdf ![]() |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB16051_A8I3e.seq ![]() |
---|
>16051_16051_B0KqF_HB-NOS_R_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GTNNNGCCNG GGTTTTCCCN GTCNCGACGT 61 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCAGT 121 TTATCATTAT CAATACTCGC CATTTCAAAG AATACGTAAA TAATTAATAG TAGTGATTTT 181 CCTAACTTTA TTTAGTCAAA AAATTAGCCT TTTAATTCTG CTGTAACCCG TACATGCCCA 241 AAATAGGGGG CGGGTTACAC AGAATATATA ACATCGTAGG TGTCTGGGTG AACAGTTTAT 301 TCCTGGCATC CACTAAATAT AATGGAGCCC GCTTTTTAAG CTGGCATCCA GAAAAAAAAA 361 GAATCCCAGC ACCAAAATAT TGTTTTCTTC ACCAACCATC AGTTCATAGG TCCATTCTCT 421 TAGCGCAACT ACAGAGAACA GGGGCACAAA CAGGCAAAAA ACGGGCACAA CCTCAATGGA 481 GTGATGCAAC CTGCCTGGAG TAAATGATGA CACAAGGCAA TTGACCCACG CATGTATCTA 541 TCTCATTTTC TTACACCTTC TATTACCTTC TGCTCTCTCT GATTTGGAAA AAGCTGAAAA 601 AAAAGGNTGA AACCAGTTCC CTGAAATTAT TCCCCTACTT GACTAATAAG TATATAAAGA 661 CNGTNNNNAT TGATTGNAAT TCTGTAAATC TATTTCTTAA ACTTCTTAAA NTCTACTTTT 721 ATAGTNAGTC NTTTTTTTTA NTTTTNAAAN NCNNNANNNN ANTTTCNNNN NNNGNNNNNN 781 NNNN // |
Primer: SV40pA3'element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3') Sequence file: RDB16051_A8I3f.seq ![]() |
>D05803_B6_A8I3_1_SV40pA3'element Reverse_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG GNNNNNCCNG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 61 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 121 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 181 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 241 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG 301 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT 361 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA 421 TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGTACC 481 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA 541 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG 601 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA 661 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA 721 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTACTTTTC 781 GTTGGGATCT TTCGAAAGGA NAGTTTGTGT CGACAGGNAA TGGTTGTCTG GNAAAANGAN 841 AGGGCCATCG CCNATNNGAN NANNNNGTNN ATAANNGNCT GCTANTTNNN NGGAANCNTC 901 TTCNNNNTNN NNNNNNNN // |
Primer: paGFP-M_R1 (Pr0893) Region: insert mid (GFP1-10 5', Pex3N, GPD pro) Sequence file: RDB16051_A8I3g.seq ![]() |
>16051_16051_B0KqF_paGFP-M_R1_C09_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNTGC CTTTTNNTGT GNNCCGGANA ACGGGAAAAG CATTGAACAC 61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA 121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA 181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA 241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC 301 CGATCTGTTC TTTGATGAAG TGTTGCTCAG TAATCCGTAA CTGCTGTTTA TACAACCATC 361 TCTTCACGAA AAACACTACC ACCGACCCTG TGGTAAATAA AGCAGCTATT CCAGTCAATG 421 AAATGAGTAC CTTTCCTCGA TGTCTCTGCA GAAGCGAACG TGATCTTTGA TTTGGGGCCA 481 TGCGGCCGCA TCCGTCGAAA CTAAGTTCTG GTGTTTTAAA ACTAAAAAAA AGACTAACTA 541 TAAAAGTAGA ATTTAAGAAG TTTAAGAAAT AGATTTACAG AATTACAATC AATACCTACC 601 GTCTTTATAT ACTTATTAGT CAAGTAGGGG AATAATTTCA GGGAACTGGT TTCAACCTTT 661 TTTTTCAGCT TTTTCCAAAT CAGAGAGAGC AGAAGGTAAT AGAAGGTGTA AGAAAATGAG 721 ATAGATACAT GCGTGGGTCA ATTGCCTTGT GTCATCATTT ACTCCAGGCA GGTTGCATCA 781 CTCCATTGAN GTTGTGCCCG TTTTTTGCCT GTTTGTGCCC CNGTTCTCTG TANTTGCGCT 841 AAGAGAATGG ANCTATGAAC TGATGGTTGG TGAANAAAAC AATATTTTGG TGNTGGGATT 901 CTTTTTTTTT TTCNGGNTGN CANCTTAAAA AAGCGGNNTN NATTATANTT NAGTGNANGN 961 CANGNANTAA ANNNNTCNNC CNNANNNCNN NNNNNNNN // |
Primer: URA3pro_F (Pr0170) Region: URA3pro, URA3 Sequence file: RDB16051_A8I3h.seq ![]() |
>D05803_B6_A8I3_1_URA3pro_F_C06_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN TNNNNNGCCN NGTATTCTTA ACCCNACTGC ACAGAACAAA 61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC 121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT 181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG 241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG 301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG 361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT 421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG 481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA 541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC 601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA 661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC 721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT 781 TGTTGGAAGA NGANTATTTG CAAAGGGAAN GGATGCTAAN GTAGAGGGTG AACGTTACAG 841 AAAAGCANGC TGGGAANCAT ATTTGAGAAN ANGCNGNNNN CAAANCTAAA AAACTGTATT 901 ATAAGTNANT GCANNNATAC TAAANNNNCN ANTTANNNCT TNNNTTTNAT TANATCANNN 961 NNNNNNN // |
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP1-10 3') Sequence file: RDB16051_A8I3i.seq ![]() |
>D05803_B6_A8I3_1_CYC1ter_R_G02_20_ABI24.ab1 1 NNNNNNNNNN NNGNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT 61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT 121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG 181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT 241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA 301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT 361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA 421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT 481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG 541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT 601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT 661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA 721 CAACTCCAGT GANNNGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC 781 CAGACCCTCC GGNTNCGATC TGTNCTTTGA TGAAGTGNTG CTCAGTAATC CGTAACTGCT 841 GTTTATACNA CCATCTCTTC ACGAAAAANN NCTACNACNN ANCNNNNNGN AAANNANNNA 901 NCNNNNNNNN NN // |
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB16051_A8I3j.seq ![]() |
>D05803_B6_A8I3_1_Amp_R_D06_12_ABI24.ab1 1 NNNNNNNNNN NNATTNNNNC NNTNATNNGG GTTATTGTCT CNTGAGCGGA NACATATTTG 61 AATGTATNNN NNNNAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT 181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC 241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC 301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA 361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT 421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG 481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC 541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA 601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA 661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG 721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGANAC GGTCACAGCT TGTCTGTAAG 781 CGGATGCCGG GAGCAGANAA GCCCGTCANG GCGCGTCAGC GGGNGTTGGC GGGTGTCGGG 841 GCTGGCTTAA CTATGCGGCA TCAGANCANA NTGTACTNAG ANTGNACCAT ACCACCTTTT 901 CAATTNATCN TTTTTTTTTT TNNNNNCNTT TTTTTNGANT TNNNNTTNCN NTNNNAANTT 961 TTTTTNNNNT NCNNNNANNN NNNNNANNNN NANNNNNNNN NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
---|
Featured content