Please review the QC test results indicated by icon below as well as clone information before placing your order.

Pex3N-GFP1-10

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: peroxisome.

Sequence (full)	RDB16051zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16051_A8I3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB16051_A8I3e.seq
>16051_16051_B0KqF_HB-NOS_R_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GTNNNGCCNG GGTTTTCCCN GTCNCGACGT    61 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCAGT   121 TTATCATTAT CAATACTCGC CATTTCAAAG AATACGTAAA TAATTAATAG TAGTGATTTT   181 CCTAACTTTA TTTAGTCAAA AAATTAGCCT TTTAATTCTG CTGTAACCCG TACATGCCCA   241 AAATAGGGGG CGGGTTACAC AGAATATATA ACATCGTAGG TGTCTGGGTG AACAGTTTAT   301 TCCTGGCATC CACTAAATAT AATGGAGCCC GCTTTTTAAG CTGGCATCCA GAAAAAAAAA   361 GAATCCCAGC ACCAAAATAT TGTTTTCTTC ACCAACCATC AGTTCATAGG TCCATTCTCT   421 TAGCGCAACT ACAGAGAACA GGGGCACAAA CAGGCAAAAA ACGGGCACAA CCTCAATGGA   481 GTGATGCAAC CTGCCTGGAG TAAATGATGA CACAAGGCAA TTGACCCACG CATGTATCTA   541 TCTCATTTTC TTACACCTTC TATTACCTTC TGCTCTCTCT GATTTGGAAA AAGCTGAAAA   601 AAAAGGNTGA AACCAGTTCC CTGAAATTAT TCCCCTACTT GACTAATAAG TATATAAAGA   661 CNGTNNNNAT TGATTGNAAT TCTGTAAATC TATTTCTTAA ACTTCTTAAA NTCTACTTTT   721 ATAGTNAGTC NTTTTTTTTA NTTTTNAAAN NCNNNANNNN ANTTTCNNNN NNNGNNNNNN 781 NNNN //
Primer: SV40pA3'element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3') Sequence file: RDB16051_A8I3f.seq
>D05803_B6_A8I3_1_SV40pA3'element Reverse_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GNNNNNCCNG CAACGCGGCC TTTTTACGGT TCCTGGCCTT    61 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG   121 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA   181 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG   241 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG   301 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT   361 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA   421 TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGTACC   481 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA   541 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG   601 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA   661 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA   721 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTACTTTTC   781 GTTGGGATCT TTCGAAAGGA NAGTTTGTGT CGACAGGNAA TGGTTGTCTG GNAAAANGAN   841 AGGGCCATCG CCNATNNGAN NANNNNGTNN ATAANNGNCT GCTANTTNNN NGGAANCNTC 901 TTCNNNNTNN NNNNNNNN //
Primer: paGFP-M_R1 (Pr0893) Region: insert mid (GFP1-10 5', Pex3N, GPD pro) Sequence file: RDB16051_A8I3g.seq
>16051_16051_B0KqF_paGFP-M_R1_C09_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTGC CTTTTNNTGT GNNCCGGANA ACGGGAAAAG CATTGAACAC    61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA   121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA   181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA   241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC   301 CGATCTGTTC TTTGATGAAG TGTTGCTCAG TAATCCGTAA CTGCTGTTTA TACAACCATC   361 TCTTCACGAA AAACACTACC ACCGACCCTG TGGTAAATAA AGCAGCTATT CCAGTCAATG   421 AAATGAGTAC CTTTCCTCGA TGTCTCTGCA GAAGCGAACG TGATCTTTGA TTTGGGGCCA   481 TGCGGCCGCA TCCGTCGAAA CTAAGTTCTG GTGTTTTAAA ACTAAAAAAA AGACTAACTA   541 TAAAAGTAGA ATTTAAGAAG TTTAAGAAAT AGATTTACAG AATTACAATC AATACCTACC   601 GTCTTTATAT ACTTATTAGT CAAGTAGGGG AATAATTTCA GGGAACTGGT TTCAACCTTT   661 TTTTTCAGCT TTTTCCAAAT CAGAGAGAGC AGAAGGTAAT AGAAGGTGTA AGAAAATGAG   721 ATAGATACAT GCGTGGGTCA ATTGCCTTGT GTCATCATTT ACTCCAGGCA GGTTGCATCA   781 CTCCATTGAN GTTGTGCCCG TTTTTTGCCT GTTTGTGCCC CNGTTCTCTG TANTTGCGCT   841 AAGAGAATGG ANCTATGAAC TGATGGTTGG TGAANAAAAC AATATTTTGG TGNTGGGATT   901 CTTTTTTTTT TTCNGGNTGN CANCTTAAAA AAGCGGNNTN NATTATANTT NAGTGNANGN 961 CANGNANTAA ANNNNTCNNC CNNANNNCNN NNNNNNNN //
Primer: URA3pro_F (Pr0170) Region: URA3pro, URA3 Sequence file: RDB16051_A8I3h.seq
>D05803_B6_A8I3_1_URA3pro_F_C06_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TNNNNNGCCN NGTATTCTTA ACCCNACTGC ACAGAACAAA    61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC   121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT   181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG   241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG   301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG   361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT   421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG   481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA   541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC   601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA   661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC   721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT   781 TGTTGGAAGA NGANTATTTG CAAAGGGAAN GGATGCTAAN GTAGAGGGTG AACGTTACAG   841 AAAAGCANGC TGGGAANCAT ATTTGAGAAN ANGCNGNNNN CAAANCTAAA AAACTGTATT   901 ATAAGTNANT GCANNNATAC TAAANNNNCN ANTTANNNCT TNNNTTTNAT TANATCANNN 961 NNNNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP1-10 3') Sequence file: RDB16051_A8I3i.seq
>D05803_B6_A8I3_1_CYC1ter_R_G02_20_ABI24.ab1     1 NNNNNNNNNN NNGNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT    61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT   121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG   181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT   241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA   301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT   361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA   421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT   481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG   541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT   601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT   661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA   721 CAACTCCAGT GANNNGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC   781 CAGACCCTCC GGNTNCGATC TGTNCTTTGA TGAAGTGNTG CTCAGTAATC CGTAACTGCT   841 GTTTATACNA CCATCTCTTC ACGAAAAANN NCTACNACNN ANCNNNNNGN AAANNANNNA 901 NCNNNNNNNN NN //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB16051_A8I3j.seq
>D05803_B6_A8I3_1_Amp_R_D06_12_ABI24.ab1     1 NNNNNNNNNN NNATTNNNNC NNTNATNNGG GTTATTGTCT CNTGAGCGGA NACATATTTG    61 AATGTATNNN NNNNAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC   121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT   181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC   241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC   301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA   361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT   421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG   481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC   541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA   601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA   661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG   721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGANAC GGTCACAGCT TGTCTGTAAG   781 CGGATGCCGG GAGCAGANAA GCCCGTCANG GCGCGTCAGC GGGNGTTGGC GGGTGTCGGG   841 GCTGGCTTAA CTATGCGGCA TCAGANCANA NTGTACTNAG ANTGNACCAT ACCACCTTTT   901 CAATTNATCN TTTTTTTTTT TNNNNNCNTT TTTTTNGANT TNNNNTTNCN NTNNNAANTT 961 TTTTTNNNNT NCNNNNANNN NNNNNANNNN NANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl