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Pex3N-GFP1-10

Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 for visualizing organelle contact sites in vivo.

Catalog number RDB16051
Resource name Pex3N-GFP1-10
Alternative name pRS316-GPDp-Pex3N-GFP1-10 (pSFL26)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae PEX3 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae PEX3 (KEGG orthology K13336) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB16051 Pex3N-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16051_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB16051_A8I3a.seq check
>D05803_B6_A8I3_1_M13_-40_A06_03_ABI24.ab1
    1 AGCAGGTAAT TGTATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA TCATTATCAA
   61 TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT AACTTTATTT
  121 AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA TAGGGGGCGG
  181 GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC TGGCATCCAC
  241 TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA TCCCAGCACC
  301 AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG CGCAACTACA
  361 GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG ATGCAACCTG
  421 CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT CATTTTCTTA
  481 CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA AGGTTGAAAC
  541 CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG TAGGTATTGA
  601 TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA GTTAGTCTTT
  661 TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCATG GCCCCAAATC
  721 AAAGATCACG TTCGCTTCTG CAGAGACATC GAGGAAAGGT ACTCATTTCA TTGACTGGAA
  781 TAGCTGCTTT ATTTACCACA GGGTCGGTGG TAGTGTTTTT CGTGAAGAGA TGGTTGTATA
  841 AACAGCAGTT ACGGATTACT GAGCAACACT TCATCAAAGA ACAGATCGGA TCCGGAGGGT
  901 CTGGTGGCGG ATCAATGGGT GGCACTAGTA TGAGCAAAGG AGAAGAACTT TTTCACTGGA
  961 GTTTGTCCCA AATTCTTTGT TTGAATTTAG ATGGGTGATG TTTAAATGGG CACAAATTTT
 1021 TCTTGTCAGA AGGAGAGGGT GACGTTGATT GCCTACCATT CGGAAAACTC ACACTAATTA
1081 TTTGCACTAC TGGCAAAACT AACC
//
Primer: CYC1ter_R
Sequence file: RDB16051_A8I3b.seq check
>D05803_B6_A8I3_1_CYC1ter_R_G02_20_ABI24.ab1
    1 ATTTTCATTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
  121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
  181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
  241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
  301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
  361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
  421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
  481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
  541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
  601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
  661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
  721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
  781 CAGACCCTCC GGATCCGATC TGTTCTTTGA TGAAGTGTTG CTCAGTAATC CGTAACTGCT
  841 GTTTATACAA CCATCTCTTC ACGAAAAAAC ACTACCACCG ACCCTGTGGT AAATAAAGCA
  901 GCTATTCCAG TCAATGAAAT GAGTACCTTT TCCCTCGATG TCTCTGCAGA AGCGAAACGT
  961 GATCTTTGAT TTGGGGGGCC ATGCGCCGCC ATCCGGTTCG AAACTAGTTC CTGCTGGTTT
 1021 TAAAACTAAA AAAGACTTAC CTATATAGTA GAAGTTCAAG AAGATTAAGG AGATAGAGTT
1081 TTACG
//
Primer: URA3pro_F
Sequence file: RDB16051_A8I3c.seq check
>D05803_B6_A8I3_1_URA3pro_F_C06_09_ABI24.ab1
    1 CAAAGCTAGT GTTGAGAACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA
   61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
  121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
  181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
  241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
  301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
  361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
  421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
  481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
  541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
  601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
  661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
  721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
  781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG
  841 AAAAGCAGGC TGGGAAGCAT ATTTGAGAAG ATGCGGCCAG CAAAACTAAA AAACTGTATT
  901 ATAAGTAAAT GCATGTATAC TAAACTCACA AATTAGAGCT TCAATTTAAT TATATCAGTT
  961 ATTACCCTAT GCGGGTGTGA AATACCGCAC AGATGCGTAA AGGAGAAAAA TACCCGCATC
 1021 ATGAAATTTG TAAGCGTTTA ATATTTTCTA AAATTCGCGA TTAATTTTTT GGTAATCAGC
1081 TCATTTTTTT AACCCAAATT AG
//
Primer: Amp_R
Sequence file: RDB16051_A8I3d.seq check
>D05803_B6_A8I3_1_Amp_R_D06_12_ABI24.ab1
    1 ACTTTTTATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
  181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
  241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
  301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
  361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
  421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
  481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
  541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
  601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
  781 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG
  841 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ACCACCTTTT
  901 CAATTCATCA TTTTTTTTTT TTTATTCTTT TTTTTTGATT TCGGTTTCCC TTTGAAATTT
  961 TTTTTTGATT TCGGTAAATC TCCGAAACAG AAAGGAAGAA CGAAAGGAAG GGAGCACAGA
 1021 CTTAGATTTG GTATATATAC GCATATGTAG TGGTGGAGAA ACATGGAAAT TGGTCCAGTA
1081 GTCGTAAACC CACTGCACAG AAGTAAAAAA CCCTTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl