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Dpp1-V5-GFP11

Expression vector of S. cerevisiae bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase (Dpp1) for visualizing organelle contact sites in vivo.

Catalog number RDB16050
Resource name Dpp1-V5-GFP11
Alternative name pRS314-GPDp-Dpp1-V5-GFP11-CYC1terb (pSFL108)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase (Dpp1) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae DPP1 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae DPP1 (KEGG orthology K18693) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB16050 Dpp1-V5-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16050_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB16050_A8I3a.seq check
>D05803_B5_A8I3_1_M13_-40_E05_14_ABI24.ab1
    1 TACCAAGGGA ATGTATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT ATCATTATCA
   61 ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC TAACTTTATT
  121 TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA ATAGGGGGCG
  181 GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC CTGGCATCCA
  241 CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA ATCCCAGCAC
  301 CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA GCGCAACTAC
  361 AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT GATGCAACCT
  421 GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC TCATTTTCTT
  481 ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA AAGGTTGAAA
  541 CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG GTAGGTATTG
  601 ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT AGTTAGTCTT
  661 TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGCCGCAT GAACAGAGTT
  721 TCGTTTATTA AAACGCCTTT CAACATAGGG GCGAAATGGA GATTAGAAGA TGTCTTTTTG
  781 CTCATTATCA TGATACTTCT TAACTACCCA GTGTATTACC AACAACCGTT CGAACGTCAG
  841 TTTTACATTA ACGATCTCAC TATATCGCAT CCTTATGCGA CAACTGAACG TGTAAATAAC
  901 AACATGTTGT TTGTTTATAG TTTTGTCGTG CCATCTTTAA CCATATTGAT AATTGGTTCC
  961 ATTTTGGCCG ATAGAAGAAC ATTTGATTTT TTATTTTTGT ACACATCTTC TCCTTTGGAT
 1021 TTAATCACTC GCTTGGATCA AGTACGGAGT TTCTTTACGA ACTTCCAATC CAAGATTGGA
1081 TTGGAAAGAC TAAGAAC
//
Primer: CYC1ter_R
Sequence file: RDB16050_A8I3b.seq check
>D05803_B5_A8I3_1_CYC1ter_R_F02_17_ABI24.ab1
    1 ACTTTCTTTC GGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TAGGTAATGC
  121 CTGCAGCATT GACATATTCG TGTAGAACCA TATGATCTCT GCTTGTGGAA CCACCACCAC
  181 TTCCACCAGT TGAGTCTAAG CCCAAAAGAG GGTTTGGTAT GGGTTTCCCA GAACCTCCGG
  241 CAGAGGATCC ACTAGTCATA CCTTCATCGG ACAAAGGATG TAATTCCTCA TCCGGGATCC
  301 TCTGATGGGT GACTGCTTCC TCCAGGGTGA CATCTGAATC GTCCATCAAT GGTTTGAACG
  361 GAAGAGGATC ATCAATGGGT GGGAAGATTC TTCTGTAGAA AAAGTGTGCC ATTATATAAC
  421 CCAACATAGA CCCTAAAATT ACATCGACGA AATGATGTCT GTAATCTTGA GTTCTGGATA
  481 GAGCAATTAG TGCAGCTCCT AACAGTGGTA GAAAGGCCAC CATTTTTCTC CATAAAGGCA
  541 TCAACGGTGA TTCAGTCAAA AGTTGCCCAC ATAGCCAGAA GTACAAATAA CCCAGTCCTG
  601 CAAAGCTTTC ACTTGAATGA CCTGACGGAG TTGTCCTAAA CCCATCCAAC AGACGTTCGT
  661 GATTCTTAGT CGTACACACA TCTTTTGCAG TAAATAAAGT GTCCAATGGC AAGCCTTCAA
  721 CAGGTTGGCA ACGATCTAGA AAATCTGGTC TTAGTCTTCC AATCCAATTC TTGATGAAGT
  781 TTGTAAAGAA ACTCGTACTG AACCAAGCGA GTGATAAACC AAGGAGAGAT GTGTACAAAA
  841 TAAAAAATCA AATGTCTTCT ATCGGCCAAA AATGGAACCA ATTATCAATA TGGTTAAAGA
  901 TGGCACGACA AAAACTATAA ACAAACAACA TGTTGTTATT TTACACGTTC AGTTTGTCGC
  961 ATAAAGGATG CGATATAGTG AGATCGTTAA TGTAAAAACT GACGTTCGAA ACGGCTTGTT
 1021 TGGTAATACA CTGCGTAGTT AAGAAAGTAT CATGGAATTA TTGAGCGAAA AGACATTCTT
1081 TTCTTATC
//
Primer: TRP1pro_F
Sequence file: RDB16050_A8I3c.seq check
>D05803_B5_A8I3_1_TRP1pro_F_G05_20_ABI24.ab1
    1 TAAGGGCATT AGCAACAAAG GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT
   61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG
  121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC
  181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC
  241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA
  301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG
  361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC
  421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG
  481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC
  541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC
  601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA
  661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT
  721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA
  781 GATGCGTAAG GAGAAAATAC CGCATCAGGA AATTGTAAGC GTTAATATTT TGTTAAAATT
  841 CGCGTTAAAT TTTTTGTTAA ATCAGCTCAT TTTTTTAACC AATAGGCCGA AATCGGCAAA
  901 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGGAA
  961 CAAGAGTCCC ACTATTAAAG AAACGTGGAC TCCAACGTCA AATGGCGAAA AACCGTCTAT
 1021 CAGGGCGGAT GGGCCCACTA CGTTGATCAT CAACCCTAAT TCAAGTTTTT GGGGATTCGA
1081 AGGTGCCGGT AAAAGCAACT AAAT
//
Primer: Amp_R
Sequence file: RDB16050_A8I3d.seq check
>D05803_B5_A8I3_1_Amp_R_H05_23_ABI24.ab1
    1 TTCGCTTTAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
  781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG
  841 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAGATCACGA
  901 CATTACTATA TATATAATAT AGGAAGCATT TAATAGAACA GCATCGTAAT ATATGTGTAC
  961 TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGGAAGATAT TCTTTTATTG AAAAAATAGC
 1021 TTGTCACTTT ACGTACATCT TGGATCAGGA GCCTTTTCCT TTTTTTTGCG ATAGAATCAA
1081 TCGGTTCGAA AAAGAAAAGG AGAGGACCAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl