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Dpp1-V5-GFP11

Expression vector of S. cerevisiae bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase (Dpp1) for visualizing organelle contact sites in vivo.

Catalog number RDB16050
Resource name Dpp1-V5-GFP11
Alternative name pRS314-GPDp-Dpp1-V5-GFP11-CYC1terb (pSFL108)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase (Dpp1) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae DPP1 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae DPP1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16050 Dpp1-V5-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16050zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16050_A8I3p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro,insert 5'(GPD pro)
          Sequence file: RDB16050_A8I3g.seq check
          >16050_16050_B2ApF_1_HB-NOS_R_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGGNNNN NNCCAGGGTT TTCCCNGTCA CGACGTTGTA
             61 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT
            121 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA
            181 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT
            241 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT
            301 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT
            361 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC
            421 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA
            481 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC
            541 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGANT TGGAAAAAGC TGAAAAAAAA
            601 GGNTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGANT AATAAGTATA TAAAGACGGT
          661 ANGNANTGAN TGNNATTCNG NAAATCNANT TCNNANNNNN NNNNN
          //
          Primer: pUC-CAPbs_up_F
          Region: lac pro,lacO,T3 pro,insert 3'(CYC1 ter, GFP11,V5 tag)
          Sequence file: RDB16050_A8I3h.seq check
          >16050_16050_B2ApF_1_pUC-CAPbs_up_F_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NCAATTAATG TGAGTTAGNN NNNNCATTAG
             61 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA
            121 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT
            181 CACTAAAGGG AACAAAAGCT GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC
            241 CTTCTCAAGC AAGGTTTTCA GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA
            301 AAGAAAAATT TGAAATATAA ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT
            361 AGGGACCTAG ACTTCAGGTT GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG
            421 AGGGCGTGAA TGTAAGCGTG ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA
            481 AGCTTGATAT CGAATTCTTA GGTAATGCCT GCAGCATTGA CATATTCGTG TAGAACCATA
            541 TGATCTCTGC TTGTGGAACC ACCACCACTT CCACCAGTTG AGTCTAAGCC CAAAAGANGG
            601 TTTGGTATGG GTTTCCCAGA NCCTCCGGCA GANGATCCNC TAGTCATACC TTCNTCNGAN
          661 NAAGGANGTA NTTCCTCNTN NGGNNNNCTC TGANGGNNGA NNGNNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid(CYC1 ter, GFP11,V5 tag,Dpp1 3')
          Sequence file: RDB16050_A8I3i.seq check
          >D05803_B5_A8I3_1_CYC1ter_R_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGNCATAACT
             61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TAGGTAATGC
            121 CTGCAGCATT GACATATTCG TGTAGAACCA TATGATCTCT GCTTGTGGAA CCACCACCAC
            181 TTCCACCAGT TGAGTCTAAG CCCAAAAGAG GGTTTGGTAT GGGTTTCCCA GAACCTCCGG
            241 CAGAGGATCC ACTAGTCATA CCTTCATCGG ACAAAGGATG TAATTCCTCA TCCGGGATCC
            301 TCTGATGGGT GACTGCTTCC TCCAGGGTGA CATCTGAATC GTCCATCAAT GGTTTGAACG
            361 GAAGAGGATC ATCAATGGGT GGGAAGATTC TTCTGTAGAA AAAGTGTGCC ATTATATAAC
            421 CCAACATAGA CCCTAAAATT ACATCGACGA AATGATGTCT GTAATCTTGA GTTCTGGATA
            481 GAGCAATTAG TGCAGCTCCT AACAGTGGTA GAAAGGCCAC CATTTTTCTC CATAAAGGCA
            541 TCAACGGTGA TTCAGTCAAA AGTTGCCCAC ATAGCCAGAA GTACAAATAA CCCAGTCCTG
            601 CAAAGCTTTC ACTTGAATGA CCTGACGGAG TTGTCCTAAA CCCATCCAAC AGACGTTCGT
            661 GATTCTTAGT CGTACACACA TCTTTTGCAG TAAATAAAGT GTCCAATGGC AAGCCTTCAA
            721 CAGGTTGGCA ACGATCTAGA AAATCTGGTC TTAGTCTTCC AATCCAATTC TTGATGAAGT
            781 TTGTAAAGAA ACTCGTACTG NACCAAGCGA GTGATAAACC AANGANANAT GTGTACAAAA
            841 TAAAAAATCA AATGTCTTCT ATCGGCCNAA AATGNAACCA ATTATCAATA TGGTTAAANN
            901 TGGNNCGACN AAANCTNTNA ANNAACNNCN TGNNGTTANT TNACNNNNNN NNNTNNNNNN
          961 NNNANNNNNN NNNN
          //
          Primer: TRP1pro_F
          Region: TRP1
          Sequence file: RDB16050_A8I3j.seq check
          >D05803_B5_A8I3_1_TRP1pro_F_G05_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNANG GCAGCTNGGN NTNNGTCTGT TATTAATTTC NCAGGTAGTT
             61 CTGGTCCATT GNNNGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA
            121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC
            181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT
            241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC
            301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA
            361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA
            421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT
            481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC
            541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG
            601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA
            661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG
            721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC
            781 AGATGCGTAA NGAGAAAATA CCGCATCANG AAATTGTAAG CGTTAATATN NTGNTAAAAT
            841 TCGCGTTAAA TTTTTNGNTA AATCAGCTCA TTTTTTTAAN NNATANGCNN AAATCGGCAA
            901 ANTCCNNTAN AANNCAAAAG NNNAGANNNA NATAGGNNNN NNNGNNGTTN NNNNTNNGNN
          961 NNNNNNNN
          //
          Primer: M13_-40
          Region: insert mid(GDP pro,Dpp1 5')
          Sequence file: RDB16050_A8I3k.seq check
          >D05803_B5_A8I3_1_M13_-40_E05_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NTNNCNATAG GGCGAANTGG AGCTCAGTTT ATCATTATCA
             61 ATACTCGCCN NNNNNNNNAN NACGTAAATA ATTAATAGTA GTGATTTTCC TAACTTTATT
            121 TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA ATAGGGGGCG
            181 GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC CTGGCATCCA
            241 CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA ATCCCAGCAC
            301 CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA GCGCAACTAC
            361 AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT GATGCAACCT
            421 GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC TCATTTTCTT
            481 ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA AAGGTTGAAA
            541 CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG GTAGGTATTG
            601 ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT AGTTAGTCTT
            661 TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGCCGCAT GAACAGAGTT
            721 TCGTTTATTA AAACGCCTTT CAACATAGGG GCGAAATGGA GATTAGAAGA NGTCTTTTTG
            781 CTCATTATCA TGATACTTCT TAACTACCCA GTGTATTACC AACAACCGTT CGAACGTCAG
            841 TTTTACATTA ACGATCTCAC TATATCGCAT CCTTATGCGA CAACNGAACG TGTAAATAAC
            901 AACANGTNGT TNGNTTATAG TTTNGTCGNN NCNTCTTTNA NCNNANNNNN NATNNNNNNC
          961 ATTTNNNNNN ANNNNANNNN NNTNNNNTTT TNNNTTTNNN NNNNNNN
          //
          Primer: Amp_R
          Region: AmpR pro,CEN_ARS
          Sequence file: RDB16050_A8I3l.seq check
          >D05803_B5_A8I3_1_Amp_R_H05_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG CANTNATNNG GGTTATTGTC TCATGAGCGG ATACATATTT
             61 GAATGTATTT ANAANAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
            121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
            181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
            241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
            301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
            361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
            421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
            481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
            541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
            601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
            661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
            721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGANA CGGTCACAGC TTGTCTGTAA
            781 GCGGATGCCG GGANCAGACA AGCCNGTCAN GGCGCGTCAG CGGGNGTTGG CGGGNGNCGG
            841 GGCTGGCTTA NCTATGCGGC ATCAGANCAN ANTGTACTGA NANTGCNCCN TANATCACGA
            901 NNTTACNATN TATNTNATAN AGGNNNNNTT TNNTANNNNN NCANCNNNNN ANNNGNNNAN
          961 NTNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl