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Dpp1-V5-GFP11 (#RDB16050)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: vacuole.

Alternative name pSFL108, pRS314-GPDp-Dpp1-V5-GFP11, pRS314-GPDp-Dpp1-V5-GFP11-CYC1terb
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase (Dpp1) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae DPP1 cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB16050zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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必要書類
提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16050 Dpp1-V5-GFP11 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Dpp1-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16050).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16050_A8I3p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800)
Region: T7 pro,insert 5'(GPD pro)
Sequence file: RDB16050_A8I3g.seq check
>16050_16050_B2ApF_1_HB-NOS_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGGNNNN NNCCAGGGTT TTCCCNGTCA CGACGTTGTA
   61 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT
  121 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA
  181 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT
  241 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT
  301 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT
  361 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC
  421 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA
  481 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC
  541 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGANT TGGAAAAAGC TGAAAAAAAA
  601 GGNTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGANT AATAAGTATA TAAAGACGGT
661 ANGNANTGAN TGNNATTCNG NAAATCNANT TCNNANNNNN NNNNN
//
Primer: pUC-CAPbs_up_F (Pr0536)
Region: lac pro,lacO,T3 pro,insert 3'(CYC1 ter, GFP11,V5 tag)
Sequence file: RDB16050_A8I3h.seq check
>16050_16050_B2ApF_1_pUC-CAPbs_up_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NCAATTAATG TGAGTTAGNN NNNNCATTAG
   61 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA
  121 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT
  181 CACTAAAGGG AACAAAAGCT GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC
  241 CTTCTCAAGC AAGGTTTTCA GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA
  301 AAGAAAAATT TGAAATATAA ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT
  361 AGGGACCTAG ACTTCAGGTT GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG
  421 AGGGCGTGAA TGTAAGCGTG ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA
  481 AGCTTGATAT CGAATTCTTA GGTAATGCCT GCAGCATTGA CATATTCGTG TAGAACCATA
  541 TGATCTCTGC TTGTGGAACC ACCACCACTT CCACCAGTTG AGTCTAAGCC CAAAAGANGG
  601 TTTGGTATGG GTTTCCCAGA NCCTCCGGCA GANGATCCNC TAGTCATACC TTCNTCNGAN
661 NAAGGANGTA NTTCCTCNTN NGGNNNNCTC TGANGGNNGA NNGNNNNN
//
Primer: CYC1ter_R (Pr0022)
Region: insert mid(CYC1 ter, GFP11,V5 tag,Dpp1 3')
Sequence file: RDB16050_A8I3i.seq check
>D05803_B5_A8I3_1_CYC1ter_R_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGNCATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TAGGTAATGC
  121 CTGCAGCATT GACATATTCG TGTAGAACCA TATGATCTCT GCTTGTGGAA CCACCACCAC
  181 TTCCACCAGT TGAGTCTAAG CCCAAAAGAG GGTTTGGTAT GGGTTTCCCA GAACCTCCGG
  241 CAGAGGATCC ACTAGTCATA CCTTCATCGG ACAAAGGATG TAATTCCTCA TCCGGGATCC
  301 TCTGATGGGT GACTGCTTCC TCCAGGGTGA CATCTGAATC GTCCATCAAT GGTTTGAACG
  361 GAAGAGGATC ATCAATGGGT GGGAAGATTC TTCTGTAGAA AAAGTGTGCC ATTATATAAC
  421 CCAACATAGA CCCTAAAATT ACATCGACGA AATGATGTCT GTAATCTTGA GTTCTGGATA
  481 GAGCAATTAG TGCAGCTCCT AACAGTGGTA GAAAGGCCAC CATTTTTCTC CATAAAGGCA
  541 TCAACGGTGA TTCAGTCAAA AGTTGCCCAC ATAGCCAGAA GTACAAATAA CCCAGTCCTG
  601 CAAAGCTTTC ACTTGAATGA CCTGACGGAG TTGTCCTAAA CCCATCCAAC AGACGTTCGT
  661 GATTCTTAGT CGTACACACA TCTTTTGCAG TAAATAAAGT GTCCAATGGC AAGCCTTCAA
  721 CAGGTTGGCA ACGATCTAGA AAATCTGGTC TTAGTCTTCC AATCCAATTC TTGATGAAGT
  781 TTGTAAAGAA ACTCGTACTG NACCAAGCGA GTGATAAACC AANGANANAT GTGTACAAAA
  841 TAAAAAATCA AATGTCTTCT ATCGGCCNAA AATGNAACCA ATTATCAATA TGGTTAAANN
  901 TGGNNCGACN AAANCTNTNA ANNAACNNCN TGNNGTTANT TNACNNNNNN NNNTNNNNNN
961 NNNANNNNNN NNNN
//
Primer: TRP1pro_F (Pr0171)
Region: TRP1
Sequence file: RDB16050_A8I3j.seq check
>D05803_B5_A8I3_1_TRP1pro_F_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNANG GCAGCTNGGN NTNNGTCTGT TATTAATTTC NCAGGTAGTT
   61 CTGGTCCATT GNNNGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA
  121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC
  181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT
  241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC
  301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA
  361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA
  421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT
  481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC
  541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG
  601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA
  661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG
  721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC
  781 AGATGCGTAA NGAGAAAATA CCGCATCANG AAATTGTAAG CGTTAATATN NTGNTAAAAT
  841 TCGCGTTAAA TTTTTNGNTA AATCAGCTCA TTTTTTTAAN NNATANGCNN AAATCGGCAA
  901 ANTCCNNTAN AANNCAAAAG NNNAGANNNA NATAGGNNNN NNNGNNGTTN NNNNTNNGNN
961 NNNNNNNN
//
Primer: M13_-40 (Pr0410)
Region: insert mid(GDP pro,Dpp1 5')
Sequence file: RDB16050_A8I3k.seq check
>D05803_B5_A8I3_1_M13_-40_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NTNNCNATAG GGCGAANTGG AGCTCAGTTT ATCATTATCA
   61 ATACTCGCCN NNNNNNNNAN NACGTAAATA ATTAATAGTA GTGATTTTCC TAACTTTATT
  121 TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA ATAGGGGGCG
  181 GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC CTGGCATCCA
  241 CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA ATCCCAGCAC
  301 CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA GCGCAACTAC
  361 AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT GATGCAACCT
  421 GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC TCATTTTCTT
  481 ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA AAGGTTGAAA
  541 CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG GTAGGTATTG
  601 ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT AGTTAGTCTT
  661 TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGCCGCAT GAACAGAGTT
  721 TCGTTTATTA AAACGCCTTT CAACATAGGG GCGAAATGGA GATTAGAAGA NGTCTTTTTG
  781 CTCATTATCA TGATACTTCT TAACTACCCA GTGTATTACC AACAACCGTT CGAACGTCAG
  841 TTTTACATTA ACGATCTCAC TATATCGCAT CCTTATGCGA CAACNGAACG TGTAAATAAC
  901 AACANGTNGT TNGNTTATAG TTTNGTCGNN NCNTCTTTNA NCNNANNNNN NATNNNNNNC
961 ATTTNNNNNN ANNNNANNNN NNTNNNNTTT TNNNTTTNNN NNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,CEN_ARS
Sequence file: RDB16050_A8I3l.seq check
>D05803_B5_A8I3_1_Amp_R_H05_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG CANTNATNNG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT ANAANAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGANA CGGTCACAGC TTGTCTGTAA
  781 GCGGATGCCG GGANCAGACA AGCCNGTCAN GGCGCGTCAG CGGGNGTTGG CGGGNGNCGG
  841 GGCTGGCTTA NCTATGCGGC ATCAGANCAN ANTGTACTGA NANTGCNCCN TANATCACGA
  901 NNTTACNATN TATNTNATAN AGGNNNNNTT TNNTANNNNN NCANCNNNNN ANNNGNNNAN
961 NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]