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Vph1-GFP1-10

Expression vector of S. cerevisiae H(+)-transporting V0 sector ATPase subunit a (Vph1) for visualizing organelle contact sites in vivo.

Catalog number RDB16049
Resource name Vph1-GFP1-10
Alternative name pRS316-GPDp-Vph1-GFP1-10 (pSFL19)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae H(+)-transporting V0 sector ATPase subunit a (Vph1) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae VPH1 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae VPH1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16049 Vph1-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16049zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16049_A8I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro, Vph1 5')
          Sequence file: RDB16049_A8I3i.seq check
          >16049_16049_B0L1F_HB-NOS_R_A09_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGGT ANGCCNGGGT TTTCCCAGTC ACGACGTTGT
             61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA
            121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT
            181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA
            241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC
            301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA
            361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG
            421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG
            481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT
            541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA
            601 AGGTTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG
            661 TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA
            721 GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCATG
            781 GCAGANAANG AGGAAGCGAT TTTTCGCTCT GCTGAAATGG CTTTAGTCCA ATTCTATATT
            841 CCTCAAGAAA TTTCAAGANA NTCTGCTTAC ACTTTANGTC NATTGNNNCT TGTTCAATTC
            901 CGNGACTNGA ACTCTAANGN NCGTGCGTTT CAAANAACNT TTCGNGAACG AAATTANAAN
            961 ANNGNNNANN GTANAAAGNN NNNNTCNNTA NNTTNNNNNN NNTTTNNNNN NANNNNNNNN
          1021 NN
          //
          Primer: SV40pA3'element Reverse
          Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
          Sequence file: RDB16049_A8I3j.seq check
          >D05803_B4_A8I3_1_SV40pA3'element Reverse_B05_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNANGGN NNNGCNNGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
             61 GCTGNNCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
            121 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
            181 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
            241 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
            301 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
            361 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG
            421 ACCATGATTA CGCCAAGCTC GAAATTAACC CTCACTAAAG GGAACAAAAG CTGGTACCGG
            481 CCGCAAATTA AAGCCTTCGA GCGTCCCAAA ACCTTCTCAA GCAAGGTTTT CAGTATAATG
            541 TTACATGCGT ACACGCGTCT GTACAGAAAA AAAAGAAAAA TTTGAAATAT AAATAACGTT
            601 CTTAATACTA ACATAACTAT AAAAAAATAA ATAGGGACCT AGACTTCAGG TTGTCTAACT
            661 CCTTCCTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
            721 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
            781 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTANTG GTTGTCTGGT AAAAGGNNAG
            841 GGNNATCGCC NATTGNNNNA NTTTNGTNNA TAATGGTCTG CNANTTNNNC GGNANNNTCT
            901 TCNNNNTNNN GGNGNANNNN NNNANNNANC TTTNNNNNCN NNNNTTTNNN TNNNNNNNNN
          961 N
          //
          Primer: paGFP-M_R1
          Region: insert mid (GFP1-10 5', Vph1 3')
          Sequence file: RDB16049_A8I3k.seq check
          >16049_16049_B0L1F_paGFP-M_R1_B09_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGNGATC CGGNNNNNNG GAAAAGCATT
             61 GAACACCATA GGTCAGAGTA GTGACAAGTG TTGGCCATGG AACAGGTAGT TTTCCAGTAG
            121 TGCAAATAAA TTTAAGTGTG AGTTTTCCGA TTGTAGCATC ACCTTCACCC TCTCCTCTGA
            181 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCAG
            241 TGAAAAGTTC TTCTCCTTTG CTCATACTAG TGCCACCCAT TGATCCGCCA CCAGACCCTC
            301 CGGATCCGCT TGAAGCGGAA GAGCTTGCAC TAGCAACAGC GACTTCCATG TCTTTATACT
            361 CAAATGCGAA TGGTTCGTAT GGTAAACCTT CACCCACGAA AAACTTGGAC ATAGATTCAA
            421 CCCAGTGCAA ACGTAAGGAA TGAAGCATGG CAGATGTACC TTCCATCAAA ACAAGAACTG
            481 CACATGTTAG TGCGAACCAC ATGGCAAAAA GTGCAACCGT CATAAACACA CCCACAAATC
            541 CTCTAAATCC AAAGGCAATT TGAATTGTCA TTGTCCATAA AACACTAGAC AATTGAGCAT
            601 GTGCCAATGA TAAGGCCCAT AAACGTAAAT AGGATGCAGT GTGCGAAACA CAATTCAAAC
            661 AGAATTCAAT TGTATGAATA ACTTGATGAA TCATAATATC ACCAAAGTCT TCACCATGAG
            721 ATCCAGAACC AACTTCTTCT TCTTCAGCGT CATCGGCGTC CATCGCGGAA ATTAATTGTT
            781 GTGCTTCCNA ATCTTCAGAA CTAGCATCTG CTTCAGTCGA TGGCAGTGGT TCGTGAGATT
            841 TCTTTTNNTG ANTGAANTTG AAATGTAATG GCTTCACCNA TAGCNACNAA NGAATACAAA
            901 CNNAGGNNNT CNACAACAAA AACACTTGGN NCTTTNCTTG NNGANGGTNN ANNTNCATCG
            961 TCNNTAGTTN CNNGGNNANA AAAACATGNT NNATCAANNN NNTNNNNNNN NCNNNNNNNN
          1021 NN
          //
          Primer: URA3pro_F
          Region: URA3 pro, URA3
          Sequence file: RDB16049_A8I3l.seq check
          >D05803_B4_A8I3_1_URA3pro_F_C05_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TGANNTGCCN NGTATTCTTA ACCCNACTGC ACAGAACAAA
             61 AACCTGNNNG NNNNGAANNN AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
            121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
            181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
            241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
            301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
            361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
            421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
            481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
            541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
            601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
            661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
            721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
            781 TGTTGGAAGA NGANTATTTG CAAAGGGAAN GGATGCTAAN GTAGAGGGTG ANCGTTACAG
            841 AAAAGCANGC TGGNAANCAT ATTTGANAAN ANGCNGNCAG CAAAACTAAA AAAACNGNAT
            901 TATAAGNAAN NGCATGTATA CTAAACTCNC AANNTANNNC NTNNNNTTNA NTANNNNNNN
          961 TNNNNNNCNN NNNNNGNNNN NNANNNNCNN NNNNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Vph1 5')
          Sequence file: RDB16049_A8I3m.seq check
          >16049_16049_B0L1F_TDH3pro_F_C10_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNC TGTNNATCTA TTTCTTAAAC TTCTTAAATT
             61 CTACTTTTAT AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT
            121 GCGGCCGCAT GGCAGAGAAG GAGGAAGCGA TTTTTCGCTC TGCTGAAATG GCTTTAGTCC
            181 AATTCTATAT TCCTCAAGAA ATTTCAAGAG ACTCTGCTTA CACTTTAGGT CAATTGGGTC
            241 TTGTTCAATT CCGTGACTTG AACTCTAAGG TGCGTGCGTT TCAAAGAACT TTCGTGAACG
            301 AAATTAGAAG ACTGGATAAT GTAGAAAGAC AGTATCGTTA CTTTTATTCT CTTTTGAAGA
            361 AACACGATAT TAAGCTCTAC GAAGGAGACA CGGACAAATA TTTGGACGGC TCAGGTGAAT
            421 TGTACGTTCC ACCAAGCGGT TCAGTGATAG ATGATTATGT CCGGAACGCT TCATATTTGG
            481 AAGAAAGATT GATTCAAATG GAGGATGCAA CCGATCAAAT TGAAGTCCAG AAAAATGACT
            541 TGGAACAGTA TCGCTTTATT TTGCAGTCAG GTGATGAATT TTTCTTGAAG GGTGATAATA
            601 CCGACAGCAC TTCCTATATG GATGAAGACA TGATCGACGC TAATGGGGAA AACATTGCTG
            661 CTGCTATCGG TGCTTCTGTA AACTATGTCA CTGGTGTCAT TGCTAGAGAC AAAGTTGCCA
            721 CCTTANAACA AATTCTTTGG AGAGTATTAA GAGGTAACCT TTTCTTCNAA ACTGTTGAAA
            781 TTGAACAACC TGTTTATGAT GTCAAAACCA GGGAGTATAA ACATAAAAAN TGCTTTTATC
            841 GTATTTTCTC ACGGNGATCT GANTATTAAA AGAANTCNNN NNAGANTGCG GNNNNATTGG
            901 ATGNCNANTC TTTACGANGT TNNNTCTNCC NNCGNNNGGT AGATCACNNN NNTTNGNNNN
          961 NNGNNCNNNN NNNNNTTNGA NNGANTNNNN NNNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP1-10, Vph1 3')
          Sequence file: RDB16049_A8I3n.seq check
          >D05803_B4_A8I3_1_CYC1ter_R_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNGNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGANATAACT
             61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
            121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
            181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
            241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
            301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
            361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
            421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
            481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
            541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
            601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
            661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
            721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
            781 CAGACCCTCC GGATCCGCTN GAAGCGGAAG ANCTTGCACT ANCAACAGCG NNTTNNATGT
            841 CTNTATACTC AAATGCGAAT GGNNCGTATN GNAAACNNNC ACCNNCNAAA AACTNNNANN
          901 TANNNNNNAC CCANNNNNAA CNNNANNNNN NNNANNNNNN GNNNNANNNN NNNNNN
          //
          Primer: Amp_R
          Region: AmpR pro,CEN_ARS
          Sequence file: RDB16049_A8I3o.seq check
          >D05803_B4_A8I3_1_Amp_R_D05_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNC NNTNATNNGG GTTATTGTCT CNTGAGCGGA NACATATTTG
             61 AATGTATNNN NNNNNATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
            181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
            241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
            301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
            361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
            421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
            481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
            541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
            601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
            661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
            721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT NNTCTGTAAG
            781 CGGATGCCGG GANCAGACAA GCNCGNCANG GCGCGNCAGC GGGNGTNGGC GGGNGNCNGG
            841 GCTGGCTNNA CTATGCNGCA TCAGANCAGA NTGNACTNNN ANNGCACCNT ACCACCNTTT
            901 CNNNTCATCA TTTTTTTTTT TANNNNTTTT TTTTTGANTT CNGNTTTNCN NNNNANNTTT
          961 TTTNNNNNNN GNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.
          user_report Tasnin, M.N., The vacuole controls nucleolar dynamics and micronucleophagy via the NVJ. Biochem. Biophys. Res. Commun. 550: 158-165 (2021). PMID 33706099.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl