Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Vph1-GFP1-10

Expression vector of S. cerevisiae H(+)-transporting V0 sector ATPase subunit a (Vph1) for visualizing organelle contact sites in vivo.

Catalog number RDB16049
Resource name Vph1-GFP1-10
Alternative name pRS316-GPDp-Vph1-GFP1-10 (pSFL19)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae H(+)-transporting V0 sector ATPase subunit a (Vph1) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae VPH1 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae VPH1 (KEGG orthology K02154) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16049 Vph1-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16049_A8I3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Region: GPD pro,insert5'
Sequence file: RDB16049_A8I3e.seq check
>D05803_B4_A8I3_1_M13_-40_A05_02_ABI24.ab1
    1 AGCAAGTAAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT
   61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA
  121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG
  181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT
  241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA
  301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG
  361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC
  421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC
  481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC
  541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT
  601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG TTAGTCTTTT
  661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGG CAGAGAAGGA
  721 GGAAGCGATT TTTCGCTCTG CTGAAATGGC TTTAGTCCAA TTCTATATTC CTCAAGAAAT
  781 TTCAAGAGAC TCTGCTTACA CTTTAGGTCA ATTGGGGTCT TGTTCAATTC CGTGACTTGA
  841 ACTCTAAGGT GCGTGCGTTT CAAAGAACTT TCGTGAACGA AATTAGAAGA CTGGATAATG
  901 TAGAAAGACA GTATCGTTAC TTTTATTCTC TTTTGAAGAA ACACGATATT AAGCTCTACG
  961 AAGGAGACAC GGACAAATAT TTGGGACGGG CTCAGTGAAA TTGTACGTTC CACCCAAGCG
 1021 GGTTCAGTGA TTAGATGAAT AATGTCCGGA ACGCTCCATA TTTGGAGAAG AATTGATTCA
1081 AATGGGGAAG GAATTG
//
Primer: CYC1ter_R
Region: CYC1 ter,insert3'
Sequence file: RDB16049_A8I3f.seq check
>D05803_B4_A8I3_1_CYC1ter_R_E02_14_ABI24.ab1
    1 ACTTCATTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
  121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
  181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
  241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
  301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
  361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
  421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
  481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
  541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
  601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
  661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
  721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
  781 CAGACCCTCC GGATCCGCTT GAAGCGGAAG AGCTTGCACT AGCAACAGCG ACTTCCATGT
  841 CTTTATACTC AAATGCGAAT GGTTCGTATG GTAAACCTTC ACCCACGAAA AACTTGGACA
  901 TAGATTCAAC CCAGTGCAAA CGTAAAGGAA TGAAAGCATG GGCAGATGTA CCCTTCCATC
  961 AAAACAGAAC TGCACATGTT TAGTGCGACC ACATGGGCAA AAAGTGCCAG CGGTTCATAA
 1021 AACACACCCC ACAATTCGTC CTAATTCGAA GTCAATTGAA TGATCAATTG TCCGATAAAC
1081 ACTAGACCAA TTGCAGCC
//
Primer: URA3pro_F
Region: URA3 pro,URA3
Sequence file: RDB16049_A8I3g.seq check
>D05803_B4_A8I3_1_URA3pro_F_C05_08_ABI24.ab1
    1 CAAGTCTGGT GTTGAGAACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA
   61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
  121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
  181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
  241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
  301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
  361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
  421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
  481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
  541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
  601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
  661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
  721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
  781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG
  841 AAAAGCAGGC TGGGAAGCAT ATTTGAGAAG ATGCGGCCAG CAAAACTAAA AAAACTGTAT
  901 TATAAGTAAA TGCATGTATA CTAAACTCAC AAATTAGAGC TTCAATTTAA TTATATCAGT
  961 TTATTACCCT ATGCGGGTGT GAAATACCCG CACAGATGCG TAAAGGAGAA AAATACCCGC
 1021 ATCATCAATT GTTAAGCGGT TAATATTTTG CTAAAAATTC CGCGATAATT TTTGCTAAAT
1081 TCAGCTCATT TTTTTAAGCA
//
Primer: Amp_R
Region: AmpR pro,CEN/ARS
Sequence file: RDB16049_A8I3h.seq check
>D05803_B4_A8I3_1_Amp_R_D05_11_ABI24.ab1
    1 TCAGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
  181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
  241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
  301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
  361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
  421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
  481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
  541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
  601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
  781 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG
  841 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ACCACCTTTT
  901 CAATTCATCA TTTTTTTTTT TATTCTTTTT TTTTTGATTT CGGGTTTCCC TTGAAATTTT
  961 TTTTGATTCG GGTAATCTCC CGAACAGAAA GAAGACGACG GATGGAGCAC AGACTTAGGA
 1021 TTGTATATAT ACGCATATGT AGTTGATGAG AAACATGAAT TGCCCAGTAT CTAACTCGAC
1081 CTGCACAGGA TGAAAAGCCT G
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl