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Vph1-GFP1-10 (#RDB16049)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: vacuole.

Alternative name pSFL19, pRS316-GPDp-Vph1-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae H(+)-transporting V0 sector ATPase subunit a (Vph1) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae VPH1 cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB16049zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

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RDB16049 Vph1-GFP1-10 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Vph1-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16049).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16049_A8I3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800)
Region: T7 pro, insert 5' (GPD pro, Vph1 5')
Sequence file: RDB16049_A8I3i.seq check
>16049_16049_B0L1F_HB-NOS_R_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGGT ANGCCNGGGT TTTCCCAGTC ACGACGTTGT
   61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA
  121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT
  181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA
  241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC
  301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA
  361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG
  421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG
  481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT
  541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA
  601 AGGTTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG
  661 TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA
  721 GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCATG
  781 GCAGANAANG AGGAAGCGAT TTTTCGCTCT GCTGAAATGG CTTTAGTCCA ATTCTATATT
  841 CCTCAAGAAA TTTCAAGANA NTCTGCTTAC ACTTTANGTC NATTGNNNCT TGTTCAATTC
  901 CGNGACTNGA ACTCTAANGN NCGTGCGTTT CAAANAACNT TTCGNGAACG AAATTANAAN
  961 ANNGNNNANN GTANAAAGNN NNNNTCNNTA NNTTNNNNNN NNTTTNNNNN NANNNNNNNN
1021 NN
//
Primer: SV40pA3'element Reverse (Pr0108)
Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
Sequence file: RDB16049_A8I3j.seq check
>D05803_B4_A8I3_1_SV40pA3'element Reverse_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNANGGN NNNGCNNGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
   61 GCTGNNCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
  121 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
  181 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
  241 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
  301 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
  361 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG
  421 ACCATGATTA CGCCAAGCTC GAAATTAACC CTCACTAAAG GGAACAAAAG CTGGTACCGG
  481 CCGCAAATTA AAGCCTTCGA GCGTCCCAAA ACCTTCTCAA GCAAGGTTTT CAGTATAATG
  541 TTACATGCGT ACACGCGTCT GTACAGAAAA AAAAGAAAAA TTTGAAATAT AAATAACGTT
  601 CTTAATACTA ACATAACTAT AAAAAAATAA ATAGGGACCT AGACTTCAGG TTGTCTAACT
  661 CCTTCCTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
  721 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
  781 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTANTG GTTGTCTGGT AAAAGGNNAG
  841 GGNNATCGCC NATTGNNNNA NTTTNGTNNA TAATGGTCTG CNANTTNNNC GGNANNNTCT
  901 TCNNNNTNNN GGNGNANNNN NNNANNNANC TTTNNNNNCN NNNNTTTNNN TNNNNNNNNN
961 N
//
Primer: paGFP-M_R1 (Pr0893)
Region: insert mid (GFP1-10 5', Vph1 3')
Sequence file: RDB16049_A8I3k.seq check
>16049_16049_B0L1F_paGFP-M_R1_B09_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGNGATC CGGNNNNNNG GAAAAGCATT
   61 GAACACCATA GGTCAGAGTA GTGACAAGTG TTGGCCATGG AACAGGTAGT TTTCCAGTAG
  121 TGCAAATAAA TTTAAGTGTG AGTTTTCCGA TTGTAGCATC ACCTTCACCC TCTCCTCTGA
  181 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCAG
  241 TGAAAAGTTC TTCTCCTTTG CTCATACTAG TGCCACCCAT TGATCCGCCA CCAGACCCTC
  301 CGGATCCGCT TGAAGCGGAA GAGCTTGCAC TAGCAACAGC GACTTCCATG TCTTTATACT
  361 CAAATGCGAA TGGTTCGTAT GGTAAACCTT CACCCACGAA AAACTTGGAC ATAGATTCAA
  421 CCCAGTGCAA ACGTAAGGAA TGAAGCATGG CAGATGTACC TTCCATCAAA ACAAGAACTG
  481 CACATGTTAG TGCGAACCAC ATGGCAAAAA GTGCAACCGT CATAAACACA CCCACAAATC
  541 CTCTAAATCC AAAGGCAATT TGAATTGTCA TTGTCCATAA AACACTAGAC AATTGAGCAT
  601 GTGCCAATGA TAAGGCCCAT AAACGTAAAT AGGATGCAGT GTGCGAAACA CAATTCAAAC
  661 AGAATTCAAT TGTATGAATA ACTTGATGAA TCATAATATC ACCAAAGTCT TCACCATGAG
  721 ATCCAGAACC AACTTCTTCT TCTTCAGCGT CATCGGCGTC CATCGCGGAA ATTAATTGTT
  781 GTGCTTCCNA ATCTTCAGAA CTAGCATCTG CTTCAGTCGA TGGCAGTGGT TCGTGAGATT
  841 TCTTTTNNTG ANTGAANTTG AAATGTAATG GCTTCACCNA TAGCNACNAA NGAATACAAA
  901 CNNAGGNNNT CNACAACAAA AACACTTGGN NCTTTNCTTG NNGANGGTNN ANNTNCATCG
  961 TCNNTAGTTN CNNGGNNANA AAAACATGNT NNATCAANNN NNTNNNNNNN NCNNNNNNNN
1021 NN
//
Primer: URA3pro_F (Pr0170)
Region: URA3 pro, URA3
Sequence file: RDB16049_A8I3l.seq check
>D05803_B4_A8I3_1_URA3pro_F_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TGANNTGCCN NGTATTCTTA ACCCNACTGC ACAGAACAAA
   61 AACCTGNNNG NNNNGAANNN AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
  121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
  181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
  241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
  301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
  361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
  421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
  481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
  541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
  601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
  661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
  721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
  781 TGTTGGAAGA NGANTATTTG CAAAGGGAAN GGATGCTAAN GTAGAGGGTG ANCGTTACAG
  841 AAAAGCANGC TGGNAANCAT ATTTGANAAN ANGCNGNCAG CAAAACTAAA AAAACNGNAT
  901 TATAAGNAAN NGCATGTATA CTAAACTCNC AANNTANNNC NTNNNNTTNA NTANNNNNNN
961 TNNNNNNCNN NNNNNGNNNN NNANNNNCNN NNNNNNNN
//
Primer: TDH3pro_F (Pr0431)
Region: insert mid (GPD pro, Vph1 5')
Sequence file: RDB16049_A8I3m.seq check
>16049_16049_B0L1F_TDH3pro_F_C10_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNC TGTNNATCTA TTTCTTAAAC TTCTTAAATT
   61 CTACTTTTAT AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT
  121 GCGGCCGCAT GGCAGAGAAG GAGGAAGCGA TTTTTCGCTC TGCTGAAATG GCTTTAGTCC
  181 AATTCTATAT TCCTCAAGAA ATTTCAAGAG ACTCTGCTTA CACTTTAGGT CAATTGGGTC
  241 TTGTTCAATT CCGTGACTTG AACTCTAAGG TGCGTGCGTT TCAAAGAACT TTCGTGAACG
  301 AAATTAGAAG ACTGGATAAT GTAGAAAGAC AGTATCGTTA CTTTTATTCT CTTTTGAAGA
  361 AACACGATAT TAAGCTCTAC GAAGGAGACA CGGACAAATA TTTGGACGGC TCAGGTGAAT
  421 TGTACGTTCC ACCAAGCGGT TCAGTGATAG ATGATTATGT CCGGAACGCT TCATATTTGG
  481 AAGAAAGATT GATTCAAATG GAGGATGCAA CCGATCAAAT TGAAGTCCAG AAAAATGACT
  541 TGGAACAGTA TCGCTTTATT TTGCAGTCAG GTGATGAATT TTTCTTGAAG GGTGATAATA
  601 CCGACAGCAC TTCCTATATG GATGAAGACA TGATCGACGC TAATGGGGAA AACATTGCTG
  661 CTGCTATCGG TGCTTCTGTA AACTATGTCA CTGGTGTCAT TGCTAGAGAC AAAGTTGCCA
  721 CCTTANAACA AATTCTTTGG AGAGTATTAA GAGGTAACCT TTTCTTCNAA ACTGTTGAAA
  781 TTGAACAACC TGTTTATGAT GTCAAAACCA GGGAGTATAA ACATAAAAAN TGCTTTTATC
  841 GTATTTTCTC ACGGNGATCT GANTATTAAA AGAANTCNNN NNAGANTGCG GNNNNATTGG
  901 ATGNCNANTC TTTACGANGT TNNNTCTNCC NNCGNNNGGT AGATCACNNN NNTTNGNNNN
961 NNGNNCNNNN NNNNNTTNGA NNGANTNNNN NNNNNN
//
Primer: CYC1ter_R (Pr0022)
Region: insert mid (CYC1 ter, GFP1-10, Vph1 3')
Sequence file: RDB16049_A8I3n.seq check
>D05803_B4_A8I3_1_CYC1ter_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGANATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
  121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
  181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
  241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
  301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
  361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
  421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
  481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
  541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
  601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
  661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
  721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
  781 CAGACCCTCC GGATCCGCTN GAAGCGGAAG ANCTTGCACT ANCAACAGCG NNTTNNATGT
  841 CTNTATACTC AAATGCGAAT GGNNCGTATN GNAAACNNNC ACCNNCNAAA AACTNNNANN
901 TANNNNNNAC CCANNNNNAA CNNNANNNNN NNNANNNNNN GNNNNANNNN NNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,CEN_ARS
Sequence file: RDB16049_A8I3o.seq check
>D05803_B4_A8I3_1_Amp_R_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNTNATNNGG GTTATTGTCT CNTGAGCGGA NACATATTTG
   61 AATGTATNNN NNNNNATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
  181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
  241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
  301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
  361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
  421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
  481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
  541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
  601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT NNTCTGTAAG
  781 CGGATGCCGG GANCAGACAA GCNCGNCANG GCGCGNCAGC GGGNGTNGGC GGGNGNCNGG
  841 GCTGGCTNNA CTATGCNGCA TCAGANCAGA NTGNACTNNN ANNGCACCNT ACCACCNTTT
  901 CNNNTCATCA TTTTTTTTTT TANNNNTTTT TTTTTGANTT CNGNTTTNCN NNNNANNTTT
961 TTTNNNNNNN GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]
user_report Tasnin, M.N., The vacuole controls nucleolar dynamics and micronucleophagy via the NVJ. Biochem. Biophys. Res. Commun. 550: 158-165 (2021). PMID 33706099. [link to RRC of NBRP]