The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: mCherry-GFP11; location: endoplasmic reticulum.
Alternative name | pSFL59, pRS314-GPDp-Ifa38-mCherry-GFP11, pRS314-GPDp-Ifa38-mCherry-GFP11-CYC1ter |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with mCherry-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS314 (plasmid) |
Selectable markers | Amp^r (E. coli), TRP1 |
Gene/insert name | S. cerevisiae IFA38 cDNA |
Depositor|Developer | Tamura, Yasushi | |
Sequence (full) ![]() |
RDB16046zzt01.seq compiled by us |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16046 | Ifa38-mCherry-GFP11 | DNA solution |
Materials & Methods section:
The Ifa38-mCherry-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16046). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16046_A8I3p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB16046_A8I3a.seq ![]() |
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>D05803B1_A8I3_2_HB-NOS_R_A06_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NNNNNCNGGG TTTTCCCNGT CACGACGTTG 61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT 121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC 181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA 241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC 301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA 361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA 421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT 481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC 541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA 601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG 661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT 721 AGTTAGTCTT TTTTTTAGTT TTAAAACACN AGAACTTAGT TTCGACGGAT GCGGCCGCTC 781 TAGAACTAGT GGATCCATGA CTTTTATGCA ACAGCTTCAA GANGCTGGGG AAAGANTTAN 841 GTGTATCAAT GGTCTTTTAT GGGTTGNTTT TGGGCTNGGN GTCTNAAANT GTACAACGTT 901 ATCACTTANA NTTTTAGCTC TNATTTTTTG ATCTTTTTTT TACTACNNGC ANNCNNNTNC 961 GANNNGNANN GNGNTNAANC NNGNTAAANN NNNNNCNNTC NNNNNNNNNN // |
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, GFP11) Sequence file: RDB16046_A8I3b.seq ![]() |
>D05803B1_A8I3_2_SV40pA3'elementReverse_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG NNNNNCNNGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT 121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG 181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG 241 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC 301 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT 361 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT 421 GACCATGATT ACGCCAAGCT CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG 481 GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT TCAGTATAAT 541 GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA TAAATAACGT 601 TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG GTTGTCTAAC 661 TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC 721 TAATTACATG ACTCGAGGTC GACTCAAGTT ATGCCGGCAG CATTGACGTA CTCGTGCAAC 781 ACCATGTGAT CCCTGCTTGT ACTCCCTCCG CCTGANCCAC CAGATCTCTT GTACAGTTCG 841 TCCATTCCAC CCGTANAANG TCTGCCTTCN GCTCGTTCAT ACTGTNNCNC AANGGTGNAA 901 TCTTCATTGT GGCTANNGAT ATNNNGCTNN NTGTNCACAT TNNACGCGCC NNNNNNCNGN 961 NNNGNNTTCN TTGNCNNNNN NNNNN // |
Primer: Ifa38-C_F (Pr0897) Region: insert mid (Ifa38 3', mCherry 5') Sequence file: RDB16046_A8I3c.seq ![]() |
>D05803B1_A8I3_2_Ifa38_C-F_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGANNNC TTTGGCGTTN ACTCTAANNN NGNNAACTCC 61 ATTAATTATT CCTTCCNTAA ATCTATCAGA ATTAGAGCCT TAAAAAAAGC CGCAAGACAG 121 GTTAAAAAGG AAAAGCTTTC CGCTGGCGGC GTTAGCAAGG GTGAGGAGGA TAACATGGCC 181 ATCATAAAGG AGTTTATGCG GTTCAAGGTA CACATGGAGG GCAGTGTCAA TGGACACGAG 241 TTTGAGATTG AGGGTGAAGG GGAAGGCCGG CCATATGAGG GGACACAAAC CGCTAAACTC 301 AAGGTGACAA AAGGAGGACC TCTGCCGTTT GCCTGGGACA TTCTGTCTCC CCAGTTCATG 361 TATGGGAGCA AGGCATACGT GAAACATCCT GCGGACATTC CAGACTACCT GAAACTCTCC 421 TTTCCCGAGG GGTTCAAATG GGAGAGGGTC ATGAACTTCG AGGACGGAGG AGTTGTCACA 481 GTGACTCAGG ACAGTTCCCT CCAAGACGGT GAGTTCATCT ACAAGGTGAA GCTGAGAGGA 541 ACCAACTTTC CTTCCGACGG GCCAGTGATG CAGAAGAAAA CGATGGGATG GGAAGCCAGC 601 TCAGAGCGTA TGTATCCCGA AGATGGTGCA CTCAAGGGCG AAATCAAACA GCGCCTGAAA 661 CTGAAGGATG GAGGCCATTA CGATGCCGAA GTCAAGACCA CCTACAAGGC AAAGAAACCC 721 GTTCAGCTTC CAGGCGCGTA TAATGTGAAC ATCAAGCTGG ATATCACTAG CCACAATGAA 781 GANTACACCA TTGTGGAACA GTATGAACGA GCCGAANGCA GACATTCTAC GGGTGGAATG 841 GACGAACTGT ACAAGAGATC TGGTGGGTCA NGCGGANGGA GTACAAGCAG GGNTCACATG 901 GNGTTGCNNG ANTACGTCAA TGCTGCCGGN ATNACTTGAN NCGNNNNNNN NNCATGTAAT 961 TANTTATGNC ANGCTNNCNT TCNCGCCNNN CCCCNNCANN NNNNNNNN // |
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB16046_A8I3d.seq ![]() |
>D05803B1_A8I3_2_TRP1pro_F_C06_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNA GGCAGCTTGG NNTNNGTCTG TTATTAATTT CNCAGGTAGT 61 TCTGGTCCAT TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA 121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC 181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT 241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC 301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA 361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA 421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT 481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC 541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG 601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA 661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG 721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC 781 AGATGCGTAA GGAGAAAATA CCGCATCANG AAATTGTAAG CGTTAATATT TTGTTAAANT 841 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCN ATANGNCGAA ATCGGCAAAA 901 TCCCTTATAA ATCNAAAGAA TAGACCGAGA TAGGGTTGAG NGNTGNTNCN GNTTGGNACN 961 AGANNCCNCT ANTAAAGAAC GNNNNNNNCN NCNNCNAANN NNNAAAAAAN NNNNNNNNN // |
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB16046_A8I3e.seq ![]() |
>D05803B1_A8I3_2_TDH3pro_F_E06_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNT GNNNTTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA 61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG 121 GCCGCTCTAG AACTAGTGGA TCCATGACTT TTATGCAACA GCTTCAAGAG GCTGGGGAAA 181 GATTTAGGTG TATCAATGGT CTTTTATGGG TTGTTTTTGG GCTTGGTGTC TTAAAATGTA 241 CAACGTTATC ACTTAGATTT TTAGCTCTTA TTTTTGATCT TTTTTTACTA CCAGCAGTCA 301 ATTTCGACAA GTATGGTGCT AAAACTGGTA AATACTGTGC TATCACTGGT GCAAGTGACG 361 GTATTGGTAA AGAATTTGCT AGACAAATGG CGAAACGTGG CTTCAACTTG GTATTGATCT 421 CGAGAACGCA ATCTAAATTG GAGGCTTTAC AAAAAGAACT AGAAGATCAA CATCATGTCG 481 TTGTAAAGAT TCTAGCCATT GACATTGCGG AGGATAAAGA ATCCAACTAT GAATCCATTA 541 AGGAATTGTG TGCGCAGTTA CCAATCACCG TTTTGGTCAA TAATGTTGGT CAATCACACT 601 CCATTCCTGT TCCATTTTTG GAAACAGAAG AGAAGGAGCT TAGAAATATT ATCACTATCA 661 ATAACACTGC TACATTATTA ATTACACAAA TCATTGCACC AAAGANTGTG GAAACTGTGA 721 AAGCTGAAAA CAAGAAGTCG GGTACTCGTG GTTTGATCTT AACCATGGGC TCATTTGGTG 781 GTCTGANTCC CACCCCACTT TTGGCTACAT ACAGNGGTTC GAAATCATTC TTACAANGTT 841 GGNCTAACTC TTTGGCTGGG GAANTATCTA AAGATGCTAT CGATGTTGAA TTNATCATTT 901 CATATTNNGN CANNANCTCN ANGNCNAAAA TCAGAANNNC ATCNTNNNNG NNNCNNAATC 961 CNNNNCAGNT NNNNAANNNN NNTTNNNNNN NNNN // |
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, mCherry 3') Sequence file: RDB16046_A8I3f.seq ![]() |
>D05803B1_A8I3_2_CYC1Ter_R_D06_12_ABI24.ab1 1 NNNNNNNNNN GGNNNNAGNG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA 61 ATTACATGAC TCGAGGTCGA CTCAAGTTAT GCCGGCAGCA TTGACGTACT CGTGCAACAC 121 CATGTGATCC CTGCTTGTAC TCCCTCCGCC TGACCCACCA GATCTCTTGT ACAGTTCGTC 181 CATTCCACCC GTAGAATGTC TGCCTTCGGC TCGTTCATAC TGTTCCACAA TGGTGTAATC 241 TTCATTGTGG CTAGTGATAT CCAGCTTGAT GTTCACATTA TACGCGCCTG GAAGCTGAAC 301 GGGTTTCTTT GCCTTGTAGG TGGTCTTGAC TTCGGCATCG TAATGGCCTC CATCCTTCAG 361 TTTCAGGCGC TGTTTGATTT CGCCCTTGAG TGCACCATCT TCGGGATACA TACGCTCTGA 421 GCTGGCTTCC CATCCCATCG TTTTCTTCTG CATCACTGGC CCGTCGGAAG GAAAGTTGGT 481 TCCTCTCAGC TTCACCTTGT AGATGAACTC ACCGTCTTGG AGGGAACTGT CCTGAGTCAC 541 TGTGACAACT CCTCCGTCCT CGAAGTTCAT GACCCTCTCC CATTTGAACC CCTCGGGAAA 601 GGAGAGTTTC AGGTAGTCTG GAATGTCCGC AGGATGTTTC ACGTATGCCT TGCTCCCATA 661 CATGAACTGG GGAGACAGAA TGTCCCAGGC AAACGGCAGA GGTCCTCCTT TTGTCACCTT 721 GAGTTTAGCG GTTTGTGTCC CCTCATATGG CCGGCCTTCC CCTTCACCCT CAATCTCAAA 781 CTCGTGTCCA TTGACACTGC CCTCCATGTG TACCTTGAAC CGCATAAACT CCTTTATGAT 841 GGCCATGTTA TCCTCCTCAN NCTTGCTAAC GCCGCCAGCG GAAAGCTTTT CCTTTTTAAC 901 CNGNCTTGNG GCTTTTTTTT NNNNNCTAAN TCTGATAGAN TTATGGNNNG NATNATTNAN 961 GNNNTTNANN NNCNNANNNT NAANGCNNAN NNNCNNNNNN NNNN // |
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB16046_A8I3g.seq ![]() |
>D05803B1_A8I3_2_Amp_R_H06_24_ABI24.ab1 1 NNNNNNNNNN NNNATTGANC NNTTATNNGG NTTATTGTCT CATGAGCGGA TACATATTTG 61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT 181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC 241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC 301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA 361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT 421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG 481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC 541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA 601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA 661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG 721 ATGACGGTGA AAACCTCTGA NACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 781 CGGANGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGNGTTGGC GGGTGTCGGG 841 GCTGGCTTAA CTATGCGGCA TCNNANCANA NTGNACTGAN AGTGCACNNT ANATCACGAN 901 NTTACTATAT ATATAATATA NGAAGCNTTT NANANAACAG CNTCGNNATA TATGNGNANN 961 TNGNNNNNNN GANGCCNNNN NNNANTANNN NNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
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