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Ifa38-mCherry-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Catalog number RDB16046
Resource name Ifa38-mCherry-GFP11
Alternative name pRS314-GPDp-Ifa38-mCherry-GFP11-CYC1ter (pSFL59)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with mCherry-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae IFA38

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks
          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16046 Ifa38-mCherry-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16046zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16046_A8I3p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro)
          Sequence file: RDB16046_A8I3a.seq check
          >D05803B1_A8I3_2_HB-NOS_R_A06_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NNNNNCNGGG TTTTCCCNGT CACGACGTTG
             61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT
            121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC
            181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA
            241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC
            301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA
            361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA
            421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT
            481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC
            541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA
            601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG
            661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT
            721 AGTTAGTCTT TTTTTTAGTT TTAAAACACN AGAACTTAGT TTCGACGGAT GCGGCCGCTC
            781 TAGAACTAGT GGATCCATGA CTTTTATGCA ACAGCTTCAA GANGCTGGGG AAAGANTTAN
            841 GTGTATCAAT GGTCTTTTAT GGGTTGNTTT TGGGCTNGGN GTCTNAAANT GTACAACGTT
            901 ATCACTTANA NTTTTAGCTC TNATTTTTTG ATCTTTTTTT TACTACNNGC ANNCNNNTNC
          961 GANNNGNANN GNGNTNAANC NNGNTAAANN NNNNNCNNTC NNNNNNNNNN
          //
          Primer: SV40pA3'Element Reverse
          Region: T3 pro, insert 3' (CYC1 ter, GFP11)
          Sequence file: RDB16046_A8I3b.seq check
          >D05803B1_A8I3_2_SV40pA3'elementReverse_B06_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNNNCNNGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
             61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
            121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
            181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
            241 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
            301 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT
            361 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT
            421 GACCATGATT ACGCCAAGCT CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG
            481 GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT TCAGTATAAT
            541 GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA TAAATAACGT
            601 TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG GTTGTCTAAC
            661 TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC
            721 TAATTACATG ACTCGAGGTC GACTCAAGTT ATGCCGGCAG CATTGACGTA CTCGTGCAAC
            781 ACCATGTGAT CCCTGCTTGT ACTCCCTCCG CCTGANCCAC CAGATCTCTT GTACAGTTCG
            841 TCCATTCCAC CCGTANAANG TCTGCCTTCN GCTCGTTCAT ACTGTNNCNC AANGGTGNAA
            901 TCTTCATTGT GGCTANNGAT ATNNNGCTNN NTGTNCACAT TNNACGCGCC NNNNNNCNGN
          961 NNNGNNTTCN TTGNCNNNNN NNNNN
          //
          Primer: Ifa38-C_F
          Region: insert mid (Ifa38 3', mCherry 5')
          Sequence file: RDB16046_A8I3c.seq check
          >D05803B1_A8I3_2_Ifa38_C-F_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGANNNC TTTGGCGTTN ACTCTAANNN NGNNAACTCC
             61 ATTAATTATT CCTTCCNTAA ATCTATCAGA ATTAGAGCCT TAAAAAAAGC CGCAAGACAG
            121 GTTAAAAAGG AAAAGCTTTC CGCTGGCGGC GTTAGCAAGG GTGAGGAGGA TAACATGGCC
            181 ATCATAAAGG AGTTTATGCG GTTCAAGGTA CACATGGAGG GCAGTGTCAA TGGACACGAG
            241 TTTGAGATTG AGGGTGAAGG GGAAGGCCGG CCATATGAGG GGACACAAAC CGCTAAACTC
            301 AAGGTGACAA AAGGAGGACC TCTGCCGTTT GCCTGGGACA TTCTGTCTCC CCAGTTCATG
            361 TATGGGAGCA AGGCATACGT GAAACATCCT GCGGACATTC CAGACTACCT GAAACTCTCC
            421 TTTCCCGAGG GGTTCAAATG GGAGAGGGTC ATGAACTTCG AGGACGGAGG AGTTGTCACA
            481 GTGACTCAGG ACAGTTCCCT CCAAGACGGT GAGTTCATCT ACAAGGTGAA GCTGAGAGGA
            541 ACCAACTTTC CTTCCGACGG GCCAGTGATG CAGAAGAAAA CGATGGGATG GGAAGCCAGC
            601 TCAGAGCGTA TGTATCCCGA AGATGGTGCA CTCAAGGGCG AAATCAAACA GCGCCTGAAA
            661 CTGAAGGATG GAGGCCATTA CGATGCCGAA GTCAAGACCA CCTACAAGGC AAAGAAACCC
            721 GTTCAGCTTC CAGGCGCGTA TAATGTGAAC ATCAAGCTGG ATATCACTAG CCACAATGAA
            781 GANTACACCA TTGTGGAACA GTATGAACGA GCCGAANGCA GACATTCTAC GGGTGGAATG
            841 GACGAACTGT ACAAGAGATC TGGTGGGTCA NGCGGANGGA GTACAAGCAG GGNTCACATG
            901 GNGTTGCNNG ANTACGTCAA TGCTGCCGGN ATNACTTGAN NCGNNNNNNN NNCATGTAAT
          961 TANTTATGNC ANGCTNNCNT TCNCGCCNNN CCCCNNCANN NNNNNNNN
          //
          Primer: TRP1pro_F
          Region: TRP1
          Sequence file: RDB16046_A8I3d.seq check
          >D05803B1_A8I3_2_TRP1pro_F_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNA GGCAGCTTGG NNTNNGTCTG TTATTAATTT CNCAGGTAGT
             61 TCTGGTCCAT TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA
            121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC
            181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT
            241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC
            301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA
            361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA
            421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT
            481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC
            541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG
            601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA
            661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG
            721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC
            781 AGATGCGTAA GGAGAAAATA CCGCATCANG AAATTGTAAG CGTTAATATT TTGTTAAANT
            841 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCN ATANGNCGAA ATCGGCAAAA
            901 TCCCTTATAA ATCNAAAGAA TAGACCGAGA TAGGGTTGAG NGNTGNTNCN GNTTGGNACN
          961 AGANNCCNCT ANTAAAGAAC GNNNNNNNCN NCNNCNAANN NNNAAAAAAN NNNNNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Ifa38 5')
          Sequence file: RDB16046_A8I3e.seq check
          >D05803B1_A8I3_2_TDH3pro_F_E06_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNNT GNNNTTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA
             61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
            121 GCCGCTCTAG AACTAGTGGA TCCATGACTT TTATGCAACA GCTTCAAGAG GCTGGGGAAA
            181 GATTTAGGTG TATCAATGGT CTTTTATGGG TTGTTTTTGG GCTTGGTGTC TTAAAATGTA
            241 CAACGTTATC ACTTAGATTT TTAGCTCTTA TTTTTGATCT TTTTTTACTA CCAGCAGTCA
            301 ATTTCGACAA GTATGGTGCT AAAACTGGTA AATACTGTGC TATCACTGGT GCAAGTGACG
            361 GTATTGGTAA AGAATTTGCT AGACAAATGG CGAAACGTGG CTTCAACTTG GTATTGATCT
            421 CGAGAACGCA ATCTAAATTG GAGGCTTTAC AAAAAGAACT AGAAGATCAA CATCATGTCG
            481 TTGTAAAGAT TCTAGCCATT GACATTGCGG AGGATAAAGA ATCCAACTAT GAATCCATTA
            541 AGGAATTGTG TGCGCAGTTA CCAATCACCG TTTTGGTCAA TAATGTTGGT CAATCACACT
            601 CCATTCCTGT TCCATTTTTG GAAACAGAAG AGAAGGAGCT TAGAAATATT ATCACTATCA
            661 ATAACACTGC TACATTATTA ATTACACAAA TCATTGCACC AAAGANTGTG GAAACTGTGA
            721 AAGCTGAAAA CAAGAAGTCG GGTACTCGTG GTTTGATCTT AACCATGGGC TCATTTGGTG
            781 GTCTGANTCC CACCCCACTT TTGGCTACAT ACAGNGGTTC GAAATCATTC TTACAANGTT
            841 GGNCTAACTC TTTGGCTGGG GAANTATCTA AAGATGCTAT CGATGTTGAA TTNATCATTT
            901 CATATTNNGN CANNANCTCN ANGNCNAAAA TCAGAANNNC ATCNTNNNNG NNNCNNAATC
          961 CNNNNCAGNT NNNNAANNNN NNTTNNNNNN NNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, mCherry 3')
          Sequence file: RDB16046_A8I3f.seq check
          >D05803B1_A8I3_2_CYC1Ter_R_D06_12_ABI24.ab1
              1 NNNNNNNNNN GGNNNNAGNG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA
             61 ATTACATGAC TCGAGGTCGA CTCAAGTTAT GCCGGCAGCA TTGACGTACT CGTGCAACAC
            121 CATGTGATCC CTGCTTGTAC TCCCTCCGCC TGACCCACCA GATCTCTTGT ACAGTTCGTC
            181 CATTCCACCC GTAGAATGTC TGCCTTCGGC TCGTTCATAC TGTTCCACAA TGGTGTAATC
            241 TTCATTGTGG CTAGTGATAT CCAGCTTGAT GTTCACATTA TACGCGCCTG GAAGCTGAAC
            301 GGGTTTCTTT GCCTTGTAGG TGGTCTTGAC TTCGGCATCG TAATGGCCTC CATCCTTCAG
            361 TTTCAGGCGC TGTTTGATTT CGCCCTTGAG TGCACCATCT TCGGGATACA TACGCTCTGA
            421 GCTGGCTTCC CATCCCATCG TTTTCTTCTG CATCACTGGC CCGTCGGAAG GAAAGTTGGT
            481 TCCTCTCAGC TTCACCTTGT AGATGAACTC ACCGTCTTGG AGGGAACTGT CCTGAGTCAC
            541 TGTGACAACT CCTCCGTCCT CGAAGTTCAT GACCCTCTCC CATTTGAACC CCTCGGGAAA
            601 GGAGAGTTTC AGGTAGTCTG GAATGTCCGC AGGATGTTTC ACGTATGCCT TGCTCCCATA
            661 CATGAACTGG GGAGACAGAA TGTCCCAGGC AAACGGCAGA GGTCCTCCTT TTGTCACCTT
            721 GAGTTTAGCG GTTTGTGTCC CCTCATATGG CCGGCCTTCC CCTTCACCCT CAATCTCAAA
            781 CTCGTGTCCA TTGACACTGC CCTCCATGTG TACCTTGAAC CGCATAAACT CCTTTATGAT
            841 GGCCATGTTA TCCTCCTCAN NCTTGCTAAC GCCGCCAGCG GAAAGCTTTT CCTTTTTAAC
            901 CNGNCTTGNG GCTTTTTTTT NNNNNCTAAN TCTGATAGAN TTATGGNNNG NATNATTNAN
          961 GNNNTTNANN NNCNNANNNT NAANGCNNAN NNNCNNNNNN NNNN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB16046_A8I3g.seq check
          >D05803B1_A8I3_2_Amp_R_H06_24_ABI24.ab1
              1 NNNNNNNNNN NNNATTGANC NNTTATNNGG NTTATTGTCT CATGAGCGGA TACATATTTG
             61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
            181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
            241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
            301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
            361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
            421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
            481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
            541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
            601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
            661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
            721 ATGACGGTGA AAACCTCTGA NACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
            781 CGGANGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGNGTTGGC GGGTGTCGGG
            841 GCTGGCTTAA CTATGCGGCA TCNNANCANA NTGNACTGAN AGTGCACNNT ANATCACGAN
            901 NTTACTATAT ATATAATATA NGAAGCNTTT NANANAACAG CNTCGNNATA TATGNGNANN
          961 TNGNNNNNNN GANGCCNNNN NNNANTANNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl