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Ifa38-mCherry-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Catalog number RDB16046
Resource name Ifa38-mCherry-GFP11
Alternative name pRS314-GPDp-Ifa38-mCherry-GFP11-CYC1ter (pSFL59)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with mCherry-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae IFA38 (KEGG orthology K10251) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Schedule A (GFP-A) [Link]
Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16046 Ifa38-mCherry-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16046_A8I3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB16046_A8I3a.seq check
>D05803B1_A8I3_2_HB-NOS_R_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NNNNNCNGGG TTTTCCCNGT CACGACGTTG
   61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT
  121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC
  181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA
  241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC
  301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA
  361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA
  421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT
  481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC
  541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA
  601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG
  661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT
  721 AGTTAGTCTT TTTTTTAGTT TTAAAACACN AGAACTTAGT TTCGACGGAT GCGGCCGCTC
  781 TAGAACTAGT GGATCCATGA CTTTTATGCA ACAGCTTCAA GANGCTGGGG AAAGANTTAN
  841 GTGTATCAAT GGTCTTTTAT GGGTTGNTTT TGGGCTNGGN GTCTNAAANT GTACAACGTT
  901 ATCACTTANA NTTTTAGCTC TNATTTTTTG ATCTTTTTTT TACTACNNGC ANNCNNNTNC
961 GANNNGNANN GNGNTNAANC NNGNTAAANN NNNNNCNNTC NNNNNNNNNN
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter, GFP11)
Sequence file: RDB16046_A8I3b.seq check
>D05803B1_A8I3_2_SV40pA3'elementReverse_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNNCNNGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
   61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
  121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
  181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
  241 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
  301 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT
  361 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT
  421 GACCATGATT ACGCCAAGCT CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG
  481 GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT TCAGTATAAT
  541 GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA TAAATAACGT
  601 TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG GTTGTCTAAC
  661 TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC
  721 TAATTACATG ACTCGAGGTC GACTCAAGTT ATGCCGGCAG CATTGACGTA CTCGTGCAAC
  781 ACCATGTGAT CCCTGCTTGT ACTCCCTCCG CCTGANCCAC CAGATCTCTT GTACAGTTCG
  841 TCCATTCCAC CCGTANAANG TCTGCCTTCN GCTCGTTCAT ACTGTNNCNC AANGGTGNAA
  901 TCTTCATTGT GGCTANNGAT ATNNNGCTNN NTGTNCACAT TNNACGCGCC NNNNNNCNGN
961 NNNGNNTTCN TTGNCNNNNN NNNNN
//
Primer: Ifa38-C_F
Region: insert mid (Ifa38 3', mCherry 5')
Sequence file: RDB16046_A8I3c.seq check
>D05803B1_A8I3_2_Ifa38_C-F_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGANNNC TTTGGCGTTN ACTCTAANNN NGNNAACTCC
   61 ATTAATTATT CCTTCCNTAA ATCTATCAGA ATTAGAGCCT TAAAAAAAGC CGCAAGACAG
  121 GTTAAAAAGG AAAAGCTTTC CGCTGGCGGC GTTAGCAAGG GTGAGGAGGA TAACATGGCC
  181 ATCATAAAGG AGTTTATGCG GTTCAAGGTA CACATGGAGG GCAGTGTCAA TGGACACGAG
  241 TTTGAGATTG AGGGTGAAGG GGAAGGCCGG CCATATGAGG GGACACAAAC CGCTAAACTC
  301 AAGGTGACAA AAGGAGGACC TCTGCCGTTT GCCTGGGACA TTCTGTCTCC CCAGTTCATG
  361 TATGGGAGCA AGGCATACGT GAAACATCCT GCGGACATTC CAGACTACCT GAAACTCTCC
  421 TTTCCCGAGG GGTTCAAATG GGAGAGGGTC ATGAACTTCG AGGACGGAGG AGTTGTCACA
  481 GTGACTCAGG ACAGTTCCCT CCAAGACGGT GAGTTCATCT ACAAGGTGAA GCTGAGAGGA
  541 ACCAACTTTC CTTCCGACGG GCCAGTGATG CAGAAGAAAA CGATGGGATG GGAAGCCAGC
  601 TCAGAGCGTA TGTATCCCGA AGATGGTGCA CTCAAGGGCG AAATCAAACA GCGCCTGAAA
  661 CTGAAGGATG GAGGCCATTA CGATGCCGAA GTCAAGACCA CCTACAAGGC AAAGAAACCC
  721 GTTCAGCTTC CAGGCGCGTA TAATGTGAAC ATCAAGCTGG ATATCACTAG CCACAATGAA
  781 GANTACACCA TTGTGGAACA GTATGAACGA GCCGAANGCA GACATTCTAC GGGTGGAATG
  841 GACGAACTGT ACAAGAGATC TGGTGGGTCA NGCGGANGGA GTACAAGCAG GGNTCACATG
  901 GNGTTGCNNG ANTACGTCAA TGCTGCCGGN ATNACTTGAN NCGNNNNNNN NNCATGTAAT
961 TANTTATGNC ANGCTNNCNT TCNCGCCNNN CCCCNNCANN NNNNNNNN
//
Primer: TRP1pro_F
Region: TRP1
Sequence file: RDB16046_A8I3d.seq check
>D05803B1_A8I3_2_TRP1pro_F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA GGCAGCTTGG NNTNNGTCTG TTATTAATTT CNCAGGTAGT
   61 TCTGGTCCAT TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA
  121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC
  181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT
  241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC
  301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA
  361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA
  421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT
  481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC
  541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG
  601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA
  661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG
  721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC
  781 AGATGCGTAA GGAGAAAATA CCGCATCANG AAATTGTAAG CGTTAATATT TTGTTAAANT
  841 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCN ATANGNCGAA ATCGGCAAAA
  901 TCCCTTATAA ATCNAAAGAA TAGACCGAGA TAGGGTTGAG NGNTGNTNCN GNTTGGNACN
961 AGANNCCNCT ANTAAAGAAC GNNNNNNNCN NCNNCNAANN NNNAAAAAAN NNNNNNNNN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, Ifa38 5')
Sequence file: RDB16046_A8I3e.seq check
>D05803B1_A8I3_2_TDH3pro_F_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNT GNNNTTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA
   61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
  121 GCCGCTCTAG AACTAGTGGA TCCATGACTT TTATGCAACA GCTTCAAGAG GCTGGGGAAA
  181 GATTTAGGTG TATCAATGGT CTTTTATGGG TTGTTTTTGG GCTTGGTGTC TTAAAATGTA
  241 CAACGTTATC ACTTAGATTT TTAGCTCTTA TTTTTGATCT TTTTTTACTA CCAGCAGTCA
  301 ATTTCGACAA GTATGGTGCT AAAACTGGTA AATACTGTGC TATCACTGGT GCAAGTGACG
  361 GTATTGGTAA AGAATTTGCT AGACAAATGG CGAAACGTGG CTTCAACTTG GTATTGATCT
  421 CGAGAACGCA ATCTAAATTG GAGGCTTTAC AAAAAGAACT AGAAGATCAA CATCATGTCG
  481 TTGTAAAGAT TCTAGCCATT GACATTGCGG AGGATAAAGA ATCCAACTAT GAATCCATTA
  541 AGGAATTGTG TGCGCAGTTA CCAATCACCG TTTTGGTCAA TAATGTTGGT CAATCACACT
  601 CCATTCCTGT TCCATTTTTG GAAACAGAAG AGAAGGAGCT TAGAAATATT ATCACTATCA
  661 ATAACACTGC TACATTATTA ATTACACAAA TCATTGCACC AAAGANTGTG GAAACTGTGA
  721 AAGCTGAAAA CAAGAAGTCG GGTACTCGTG GTTTGATCTT AACCATGGGC TCATTTGGTG
  781 GTCTGANTCC CACCCCACTT TTGGCTACAT ACAGNGGTTC GAAATCATTC TTACAANGTT
  841 GGNCTAACTC TTTGGCTGGG GAANTATCTA AAGATGCTAT CGATGTTGAA TTNATCATTT
  901 CATATTNNGN CANNANCTCN ANGNCNAAAA TCAGAANNNC ATCNTNNNNG NNNCNNAATC
961 CNNNNCAGNT NNNNAANNNN NNTTNNNNNN NNNN
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, mCherry 3')
Sequence file: RDB16046_A8I3f.seq check
>D05803B1_A8I3_2_CYC1Ter_R_D06_12_ABI24.ab1
    1 NNNNNNNNNN GGNNNNAGNG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CTCAAGTTAT GCCGGCAGCA TTGACGTACT CGTGCAACAC
  121 CATGTGATCC CTGCTTGTAC TCCCTCCGCC TGACCCACCA GATCTCTTGT ACAGTTCGTC
  181 CATTCCACCC GTAGAATGTC TGCCTTCGGC TCGTTCATAC TGTTCCACAA TGGTGTAATC
  241 TTCATTGTGG CTAGTGATAT CCAGCTTGAT GTTCACATTA TACGCGCCTG GAAGCTGAAC
  301 GGGTTTCTTT GCCTTGTAGG TGGTCTTGAC TTCGGCATCG TAATGGCCTC CATCCTTCAG
  361 TTTCAGGCGC TGTTTGATTT CGCCCTTGAG TGCACCATCT TCGGGATACA TACGCTCTGA
  421 GCTGGCTTCC CATCCCATCG TTTTCTTCTG CATCACTGGC CCGTCGGAAG GAAAGTTGGT
  481 TCCTCTCAGC TTCACCTTGT AGATGAACTC ACCGTCTTGG AGGGAACTGT CCTGAGTCAC
  541 TGTGACAACT CCTCCGTCCT CGAAGTTCAT GACCCTCTCC CATTTGAACC CCTCGGGAAA
  601 GGAGAGTTTC AGGTAGTCTG GAATGTCCGC AGGATGTTTC ACGTATGCCT TGCTCCCATA
  661 CATGAACTGG GGAGACAGAA TGTCCCAGGC AAACGGCAGA GGTCCTCCTT TTGTCACCTT
  721 GAGTTTAGCG GTTTGTGTCC CCTCATATGG CCGGCCTTCC CCTTCACCCT CAATCTCAAA
  781 CTCGTGTCCA TTGACACTGC CCTCCATGTG TACCTTGAAC CGCATAAACT CCTTTATGAT
  841 GGCCATGTTA TCCTCCTCAN NCTTGCTAAC GCCGCCAGCG GAAAGCTTTT CCTTTTTAAC
  901 CNGNCTTGNG GCTTTTTTTT NNNNNCTAAN TCTGATAGAN TTATGGNNNG NATNATTNAN
961 GNNNTTNANN NNCNNANNNT NAANGCNNAN NNNCNNNNNN NNNN
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB16046_A8I3g.seq check
>D05803B1_A8I3_2_Amp_R_H06_24_ABI24.ab1
    1 NNNNNNNNNN NNNATTGANC NNTTATNNGG NTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
  181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
  241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
  301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
  361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
  421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
  481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
  541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
  601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA NACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
  781 CGGANGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGNGTTGGC GGGTGTCGGG
  841 GCTGGCTTAA CTATGCGGCA TCNNANCANA NTGNACTGAN AGTGCACNNT ANATCACGAN
  901 NTTACTATAT ATATAATATA NGAAGCNTTT NANANAACAG CNTCGNNATA TATGNGNANN
961 TNGNNNNNNN GANGCCNNNN NNNANTANNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2021.03.11

GNP_filter3_RDBDEP_html_210308.pl