Please review the QC test results indicated by icon below as well as clone information before placing your order.

Ifa38-FLAG-GFP11

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: 3xFLAG-GFP11; location: endoplasmic reticulum.

Sequence (full)	RDB16045zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16045_A8I3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R Region: T7 pro, insert 5' (GPD pro, Ifa38 5') Sequence file: RDB16045_A8I3g.seq
>16045_16045_B0KqF_HB-NOS_R_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGTNNNGCC NGGGTTTTCC CNGTCACGAC    61 GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCA   121 GTTTATCATT ATCAATACTC GCCATTTCAA AGAATACGTA AATAATTAAT AGTAGTGATT   181 TTCCTAACTT TATTTAGTCA AAAAATTAGC CTTTTAATTC TGCTGTAACC CGTACATGCC   241 CAAAATAGGG GGCGGGTTAC ACAGAATATA TAACATCGTA GGTGTCTGGG TGAACAGTTT   301 ATTCCTGGCA TCCACTAAAT ATAATGGAGC CCGCTTTTTA AGCTGGCATC CAGAAAAAAA   361 AAGAATCCCA GCACCAAAAT ATTGTTTTCT TCACCAACCA TCAGTTCATA GGTCCATTCT   421 CTTAGCGCAA CTACAGAGAA CAGGGGCACA AACAGGCAAA AAACGGGCAC AACCTCAATG   481 GAGTGATGCA ACCTGCCTGG AGTAAATGAT GACACAAGGC AATTGACCCA CGCATGTATC   541 TATCTCATTT TCTTACACCT TCTATTACCT TCTGCTCTCT CTGATTTGGA AAAAGCTGAA   601 AAAAAAGGTT GAAACCAGTT CCCTGAAATT ATTCCCCTAC TTGACTAATA AGTATATAAA   661 GACGGTAGGT ATTGATTGTA ATTCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT   721 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC GGATGCGGCC   781 GCATGACTTT TATGCAACAG CTTCAAGANG CTGGGGAAAG ATTTANGTGT ATCAATGGTC   841 TTTTATGGGT TGTTTTTGGG CTTGGTGTCT TAAAATGTAC NACGTTATCA NTTAGATTTT   901 TTAGCTCTTA NTTTTTGANC TTTTTTTTTA CTANNAGNAN NNNANTTTCN NNNAGTATNG 961 NTGCTNAAAA NNNGNAAATA CTGNNNNATC ANNGNNNNNN NNNN //
Primer: SV40pA3'element Reverse Region: T3 pro, insert 3' (CYC1 ter, 3xFLAG-GFP11 3') Sequence file: RDB16045_A8I3h.seq
>D05803_A9_A8I3_1_SV40pA3'element Reverse_B04_04_ABI24.ab1     1 NNNNNNGNNN NNNNNNNNNN NGNNNNNNGC CNGNAACGCG GCCTTTTNAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA   121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG   181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG   241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA   301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT   361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG   421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT   481 ACCGGGCCCG GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT   541 TCAGTATAAT GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA   601 TAAATAACGT TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG   661 GTTGTCTAAC TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC   721 GTGACATAAC TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC   781 TTATGTTATC CCTGCAGCAT TGACGTATTC ATGCAAAACC ATGTGATCTC NGGAAGTTGA   841 ACCTCNACCA CTNCCGCCTT TATCGNCATC ATCCTTATAN NCAATGNCAN GATNCTNATA   901 ATCNCCNTCA NNGNCTTNNN AATCACCNNN GGNNNANGNA NNCNNNNNTT TTTNNNNNNN 961 NNN //
Primer: CYC1ter_R Region: insert mid (CYC ter, 3xFLAG-GFP11, Ifa38 3') Sequence file: RDB16045_A8I3i.seq
>D05803_A9_A8I3_1_CYC1ter_R_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA    61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC   121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT   181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG   241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTCCTTTTTA ACCTGTCTTG CGGCTTTTTT   301 TAAGGCTCTA ATTCTGATAG ATTTATGGAA GGAATAATTA ATGGAGTTAA CAATCTTAGA   361 GTAAACGCCA AAGGTCTCTG TAATTACAAA TTGATACACT GCGTGCGCCC AGTAAGGGGT   421 CATAGTAGCG TATCTTTCTT GAGAGCCGCA GCGTCTGCCA ACGCTTCTTA AAGTGGATTT   481 TACAAACTGT TGTGGATTTG GGATCATTAA AGATGATCTT CTGATTTTAG ACATCGAGCT   541 AGTGACCAAA TATGAAATGA TTAATTCAAC ATCGATAGCA TCTTTAGATA ATTCCCCAGC   601 CAAAGAGTTA GACCAACCTT GTAAGAATGA TTTCGAACCA CTGTATGTAG CCAAAAGTGG   661 GGTGGGAATC AGANCACCAA ATGAGCCCAT GGTTAAGATC AAACCACGAG TACCCGACTT   721 CTTGTTTTCA GCTTTCACAG TTTCCACAAT CTTTGGTGCA ATGATTTGTG TAATTAATAA   781 TGTAGCAGTG TTATTGATAG TGANAATATT TCTAAGCTCC TTCTCTTCTG TNTCCNAAAA   841 TGNNACANGA ANGGNNNGTG NNNNANNNAN ATTATNGANN NAAANNGGNN NNNNGNANCN 901 GCGNNCACAA TTCCTNNNNN GNNNTNNNNN NNNNN //
Primer: HIS3pro_F Region: HIS3 pro, HIS3 Sequence file: RDB16045_A8I3j.seq
>D05803_A9_A8I3_1_HIS3pro_F_C04_07_ABI24.ab1     1 NNNNNNNNNN NNTNNNNNNA AGTAANGTGN NTTCTTCGAA NAANATACTA AAAAATGAGC    61 AGGCAAGATA AACGAAGGCA AAGATGACAG AGCAGAAAGC CCTAGTAAAG CGTATTACAA   121 ATGAAACCAA GATTCAGATT GCGATCTCTT TAAAGGGTGG TCCCCTAGCG ATAGAGCACT   181 CGATCTTCCC AGAAAAAGAG GCAGAAGCAG TAGCAGAACA GGCCACACAA TCGCAAGTGA   241 TTAACGTCCA CACAGGTATA GGGTTTCTGG ACCATATGAT ACATGCTCTG GCCAAGCATT   301 CCGGCTGGTC GCTAATCGTT GAGTGCATTG GTGACTTACA CATAGACGAC CATCACACCA   361 CTGAAGACTG CGGGATTGCT CTCGGTCAAG CTTTTAAAGA GGCCCTAGGG GCCGTGCGTG   421 GAGTAAAAAG GTTTGGATCA GGATTTGCGC CTTTGGATGA GGCACTTTCC AGAGCGGTGG   481 TAGATCTTTC GAACAGGCCG TACGCAGTTG TCGAACTTGG TTTGCAAAGG GAGAAAGTAG   541 GAGATCTCTC TTGCGAGATG ATCCCGCATT TTCTTGAAAG CTTTGCAGAG GCTAGCAGAA   601 TTACCCTCCA CGTTGATTGT CTGCGAGGCA AGAATGATCA TCACCGTAGT GAGAGTGCGT   661 TCAAGGCTCT TGCGGTTGCC ATAAGAGAAG CCACCTCGCC CAATGGTACC AACGATGTTC   721 CCTCCACCAA AGGTGTTCTT ATGTAGTGAC ACCGATNATT TAAAGCTGCA GCATACGATA   781 TATATACNTG TGTATATATG TATACCTATG AATGTCAGTA NGTATGTATA CGAACAGTAT   841 GATACTGAAG ATGANAAGGT AATGCATCAT TCTATACGNG NCANNCNGAA NGANGNGCGC   901 TTTCCNTTTT TTCNTTTTNG NTTTTTNCNT TTTTTTTTNC NNNNGNNNNC NNCNGNNNNN 961 NNNNN //
Primer: TDH3pro_F Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB16045_A8I3k.seq
>16045_16045_B0KqF_TDH3pro_F_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT GNNATTCNGT AAATCTATTT CTTAAACTTC TTAAATTCTA    61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG   121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG   181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT   241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG   301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG   361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT   421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA   481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT   541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT   601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT   661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT   721 CGGGTACTCG TGGTTTGATC TTAACCATGG GCTCATTTGG TGGTCTGATT CCCACCCCAC   781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACAAGG TTGGTCTAAC TCTTTGGCTG   841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTCATATTTG GTCACTANCT   901 CNANGNCNNA AANCAGAAGA TCATCTTTNA NGATCCNNAA TCCNNNANNN TTTNNNAAAN 961 TCCNNNTTNN ANNNNNNNNN N //
Primer: Amp_R Region: Amp pro, CEN_ARS Sequence file: RDB16045_A8I3l.seq
>D05803_A9_A8I3_1_Amp_R_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNATTNNNC NNTNATNNGG GTTANTGTCT CNTGANCGGA TACATATTTG    61 AATGTATTNN NNNNAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC   121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT   181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA   241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT   301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT   361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT   421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA   481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT   541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT   601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA   661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG   721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGANNN GGTCACAGCT TGTCTGTAAG   781 CGGATGCCGG GAGCAGANAA GCCCGTCANG GCGCGTCAGC GGGNNTTGGC GGGNGTCNGG   841 GGCTGGCTTA ACTATGCGGC ATCAGANCAG ANTGTACTGA GANTGCACCA TAANNTCCCN 901 NTTTAANANC TNGNNNANCG CTNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl