Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Ifa38-FLAG-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Catalog number RDB16045
Resource name Ifa38-FLAG-GFP11
Alternative name pRS313-GPDp-Ifa38-3xFLAG-GFP11 (pSFL86)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS313 (plasmid)
Selectable markers Amp^r (E. coli), HIS3
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae IFA38 (KEGG orthology K10251) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16045 Ifa38-FLAG-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16045_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB16045_A8I3a.seq check
>D05803_A9_A8I3_1_M13_-40_A04_01_ABI24.ab1
    1 TGCCAAGCAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT
   61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA
  121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG
  181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT
  241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA
  301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG
  361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC
  421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC
  481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC
  541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT
  601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG TTAGTCTTTT
  661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGA CTTTTATGCA
  721 ACAGCTTCAA GAGGCTGGGG AAAGATTTAG GTGTATCAAT GGTCTTTTAT GGGTTGTTTT
  781 TGGGCTTGGT GTCTTAAAAT GTACAACGTT ATCACTTAGA TTTTTAGCTC TTATTTTTGA
  841 TCTTTTTTTA CTACCAGCAG TCAATTTCGA CAAGTATGGT GCTAAAACTG GTAAATACTG
  901 TGCTATCACT GGTGCAAGTG ACGGTATTGG TAAAGAATTT GCTAGACAAA TGGCGAAACG
  961 TGGCTTCAAC TTGGGTATTG ATCTCGAGAA CGCAATCTAA ATTGGGAGGC TTTACAAAAA
 1021 GAAACTAGAG AATCACCATC CATGTTCGGT TGTAAAAGGA TCTAAGTCAA TGACCATTGG
1081 CGGAAGGATA AAAAGTCCTA
//
Primer: CYC1ter_R
Sequence file: RDB16045_A8I3b.seq check
>D05803_A9_A8I3_1_CYC1ter_R_A02_02_ABI24.ab1
    1 CCTTCGTTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC
  121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT
  181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG
  241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTCCTTTTTA ACCTGTCTTG CGGCTTTTTT
  301 TAAGGCTCTA ATTCTGATAG ATTTATGGAA GGAATAATTA ATGGAGTTAA CAATCTTAGA
  361 GTAAACGCCA AAGGTCTCTG TAATTACAAA TTGATACACT GCGTGCGCCC AGTAAGGGGT
  421 CATAGTAGCG TATCTTTCTT GAGAGCCGCA GCGTCTGCCA ACGCTTCTTA AAGTGGATTT
  481 TACAAACTGT TGTGGATTTG GGATCATTAA AGATGATCTT CTGATTTTAG ACATCGAGCT
  541 AGTGACCAAA TATGAAATGA TTAATTCAAC ATCGATAGCA TCTTTAGATA ATTCCCCAGC
  601 CAAAGAGTTA GACCAACCTT GTAAGAATGA TTTCGAACCA CTGTATGTAG CCAAAAGTGG
  661 GGTGGGAATC AGACCACCAA ATGAGCCCAT GGTTAAGATC AAACCACGAG TACCCGACTT
  721 CTTGTTTTCA GCTTTCACAG TTTCCACAAT CTTTGGTGCA ATGATTTGTG TAATTAATAA
  781 TGTAGCAGTG TTATTGATAG TGATAATATT TCTAAGCTCC TTCTCTTCTG TTTCCAAAAA
  841 TGGAACAGGA ATGGAGTGTG ATTGACCAAC ATTATTGACC AAAAACGGTG ATTGGTAACT
  901 GCGCACACAA TTCCTTAATG GGATTCATAG TTGGATTCTT TATCCTCCGC AAATGTCAAT
  961 GGGCTAGAAA TCTTTACACG ACATGATGTT GATCTTCTAC ACCTTTTTGT AAAGCTCCAA
 1021 TTTAGAATGC GATTCTCGAG ATCCATTACT AGCTGCAGCC AACGTTCGCG ATTGTCCTAG
1081 CAAA
//
Primer: HIS3pro_F
Sequence file: RDB16045_A8I3c.seq check
>D05803_A9_A8I3_1_HIS3pro_F_C04_07_ABI24.ab1
    1 AGTCATAATA CATTATATAA AGTAATGTGA TTTCTTCGAA GAATATACTA AAAAATGAGC
   61 AGGCAAGATA AACGAAGGCA AAGATGACAG AGCAGAAAGC CCTAGTAAAG CGTATTACAA
  121 ATGAAACCAA GATTCAGATT GCGATCTCTT TAAAGGGTGG TCCCCTAGCG ATAGAGCACT
  181 CGATCTTCCC AGAAAAAGAG GCAGAAGCAG TAGCAGAACA GGCCACACAA TCGCAAGTGA
  241 TTAACGTCCA CACAGGTATA GGGTTTCTGG ACCATATGAT ACATGCTCTG GCCAAGCATT
  301 CCGGCTGGTC GCTAATCGTT GAGTGCATTG GTGACTTACA CATAGACGAC CATCACACCA
  361 CTGAAGACTG CGGGATTGCT CTCGGTCAAG CTTTTAAAGA GGCCCTAGGG GCCGTGCGTG
  421 GAGTAAAAAG GTTTGGATCA GGATTTGCGC CTTTGGATGA GGCACTTTCC AGAGCGGTGG
  481 TAGATCTTTC GAACAGGCCG TACGCAGTTG TCGAACTTGG TTTGCAAAGG GAGAAAGTAG
  541 GAGATCTCTC TTGCGAGATG ATCCCGCATT TTCTTGAAAG CTTTGCAGAG GCTAGCAGAA
  601 TTACCCTCCA CGTTGATTGT CTGCGAGGCA AGAATGATCA TCACCGTAGT GAGAGTGCGT
  661 TCAAGGCTCT TGCGGTTGCC ATAAGAGAAG CCACCTCGCC CAATGGTACC AACGATGTTC
  721 CCTCCACCAA AGGTGTTCTT ATGTAGTGAC ACCGATTATT TAAAGCTGCA GCATACGATA
  781 TATATACATG TGTATATATG TATACCTATG AATGTCAGTA AGTATGTATA CGAACAGTAT
  841 GATACTGAAG ATGACAAGGT AATGCATCAT TCTATACGTG TCATTCTGAA CGAGGCGCGC
  901 TTTCCTTTTT TTCTTTTTTG CTTTTTTCTT TTTTTTTTTC TCTTGAACTC GACGGGATCT
  961 ATGCGGGTGT GAAAATACCC GCACAGATGC GTAAGGAGAA AAATACCCGC ATCAGGAAAT
 1021 TTGTAAGCGT TAATATTTTT GTTTAAAAAT TCGCGTTAAT TTTTGATTAA TTCAGCTTCA
1081 TTTTTTAACC GATAGCCGAA ATTCCGGCCA
//
Primer: Amp_R
Sequence file: RDB16045_A8I3d.seq check
>D05803_A9_A8I3_1_Amp_R_D04_10_ABI24.ab1
    1 TCATTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT
  181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA
  241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT
  301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT
  361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT
  421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA
  481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT
  541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT
  601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
  781 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG
  841 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAAATTCCCG
  901 TTTTAAGAGC TTGGTGAGCG CTAGGAGTCA CTGCCAGGTA TCGTTTGAAC ACGGCATTAG
  961 TCAGGAAAGT CATAACACAG TCCCTTTCCC GCAATTTTCT TTTTTTCTAT AACTCTTGAT
 1021 CCTCCTCTAG TTACACTTCT ATATTTTTTT ATTGCCTCGT AATGGATTTT CCATTTAAAT
1081 ATTTCCACCG TAAGTCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl