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Ifa38-FLAG-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Catalog number RDB16045
Resource name Ifa38-FLAG-GFP11
Alternative name pRS313-GPDp-Ifa38-3xFLAG-GFP11 (pSFL86)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS313 (plasmid)
Selectable markers Amp^r (E. coli), HIS3
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae IFA38 (KEGG orthology K10251) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
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RDB16045 Ifa38-FLAG-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16045_A8I3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro, Ifa38 5')
Sequence file: RDB16045_A8I3g.seq check
>16045_16045_B0KqF_HB-NOS_R_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGTNNNGCC NGGGTTTTCC CNGTCACGAC
   61 GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCA
  121 GTTTATCATT ATCAATACTC GCCATTTCAA AGAATACGTA AATAATTAAT AGTAGTGATT
  181 TTCCTAACTT TATTTAGTCA AAAAATTAGC CTTTTAATTC TGCTGTAACC CGTACATGCC
  241 CAAAATAGGG GGCGGGTTAC ACAGAATATA TAACATCGTA GGTGTCTGGG TGAACAGTTT
  301 ATTCCTGGCA TCCACTAAAT ATAATGGAGC CCGCTTTTTA AGCTGGCATC CAGAAAAAAA
  361 AAGAATCCCA GCACCAAAAT ATTGTTTTCT TCACCAACCA TCAGTTCATA GGTCCATTCT
  421 CTTAGCGCAA CTACAGAGAA CAGGGGCACA AACAGGCAAA AAACGGGCAC AACCTCAATG
  481 GAGTGATGCA ACCTGCCTGG AGTAAATGAT GACACAAGGC AATTGACCCA CGCATGTATC
  541 TATCTCATTT TCTTACACCT TCTATTACCT TCTGCTCTCT CTGATTTGGA AAAAGCTGAA
  601 AAAAAAGGTT GAAACCAGTT CCCTGAAATT ATTCCCCTAC TTGACTAATA AGTATATAAA
  661 GACGGTAGGT ATTGATTGTA ATTCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT
  721 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC GGATGCGGCC
  781 GCATGACTTT TATGCAACAG CTTCAAGANG CTGGGGAAAG ATTTANGTGT ATCAATGGTC
  841 TTTTATGGGT TGTTTTTGGG CTTGGTGTCT TAAAATGTAC NACGTTATCA NTTAGATTTT
  901 TTAGCTCTTA NTTTTTGANC TTTTTTTTTA CTANNAGNAN NNNANTTTCN NNNAGTATNG
961 NTGCTNAAAA NNNGNAAATA CTGNNNNATC ANNGNNNNNN NNNN
//
Primer: SV40pA3'element Reverse
Region: T3 pro, insert 3' (CYC1 ter, 3xFLAG-GFP11 3')
Sequence file: RDB16045_A8I3h.seq check
>D05803_A9_A8I3_1_SV40pA3'element Reverse_B04_04_ABI24.ab1
    1 NNNNNNGNNN NNNNNNNNNN NGNNNNNNGC CNGNAACGCG GCCTTTTNAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
  301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
  361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
  421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
  481 ACCGGGCCCG GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT
  541 TCAGTATAAT GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA
  601 TAAATAACGT TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG
  661 GTTGTCTAAC TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC
  721 GTGACATAAC TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC
  781 TTATGTTATC CCTGCAGCAT TGACGTATTC ATGCAAAACC ATGTGATCTC NGGAAGTTGA
  841 ACCTCNACCA CTNCCGCCTT TATCGNCATC ATCCTTATAN NCAATGNCAN GATNCTNATA
  901 ATCNCCNTCA NNGNCTTNNN AATCACCNNN GGNNNANGNA NNCNNNNNTT TTTNNNNNNN
961 NNN
//
Primer: CYC1ter_R
Region: insert mid (CYC ter, 3xFLAG-GFP11, Ifa38 3')
Sequence file: RDB16045_A8I3i.seq check
>D05803_A9_A8I3_1_CYC1ter_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC
  121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT
  181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG
  241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTCCTTTTTA ACCTGTCTTG CGGCTTTTTT
  301 TAAGGCTCTA ATTCTGATAG ATTTATGGAA GGAATAATTA ATGGAGTTAA CAATCTTAGA
  361 GTAAACGCCA AAGGTCTCTG TAATTACAAA TTGATACACT GCGTGCGCCC AGTAAGGGGT
  421 CATAGTAGCG TATCTTTCTT GAGAGCCGCA GCGTCTGCCA ACGCTTCTTA AAGTGGATTT
  481 TACAAACTGT TGTGGATTTG GGATCATTAA AGATGATCTT CTGATTTTAG ACATCGAGCT
  541 AGTGACCAAA TATGAAATGA TTAATTCAAC ATCGATAGCA TCTTTAGATA ATTCCCCAGC
  601 CAAAGAGTTA GACCAACCTT GTAAGAATGA TTTCGAACCA CTGTATGTAG CCAAAAGTGG
  661 GGTGGGAATC AGANCACCAA ATGAGCCCAT GGTTAAGATC AAACCACGAG TACCCGACTT
  721 CTTGTTTTCA GCTTTCACAG TTTCCACAAT CTTTGGTGCA ATGATTTGTG TAATTAATAA
  781 TGTAGCAGTG TTATTGATAG TGANAATATT TCTAAGCTCC TTCTCTTCTG TNTCCNAAAA
  841 TGNNACANGA ANGGNNNGTG NNNNANNNAN ATTATNGANN NAAANNGGNN NNNNGNANCN
901 GCGNNCACAA TTCCTNNNNN GNNNTNNNNN NNNNN
//
Primer: HIS3pro_F
Region: HIS3 pro, HIS3
Sequence file: RDB16045_A8I3j.seq check
>D05803_A9_A8I3_1_HIS3pro_F_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNTNNNNNNA AGTAANGTGN NTTCTTCGAA NAANATACTA AAAAATGAGC
   61 AGGCAAGATA AACGAAGGCA AAGATGACAG AGCAGAAAGC CCTAGTAAAG CGTATTACAA
  121 ATGAAACCAA GATTCAGATT GCGATCTCTT TAAAGGGTGG TCCCCTAGCG ATAGAGCACT
  181 CGATCTTCCC AGAAAAAGAG GCAGAAGCAG TAGCAGAACA GGCCACACAA TCGCAAGTGA
  241 TTAACGTCCA CACAGGTATA GGGTTTCTGG ACCATATGAT ACATGCTCTG GCCAAGCATT
  301 CCGGCTGGTC GCTAATCGTT GAGTGCATTG GTGACTTACA CATAGACGAC CATCACACCA
  361 CTGAAGACTG CGGGATTGCT CTCGGTCAAG CTTTTAAAGA GGCCCTAGGG GCCGTGCGTG
  421 GAGTAAAAAG GTTTGGATCA GGATTTGCGC CTTTGGATGA GGCACTTTCC AGAGCGGTGG
  481 TAGATCTTTC GAACAGGCCG TACGCAGTTG TCGAACTTGG TTTGCAAAGG GAGAAAGTAG
  541 GAGATCTCTC TTGCGAGATG ATCCCGCATT TTCTTGAAAG CTTTGCAGAG GCTAGCAGAA
  601 TTACCCTCCA CGTTGATTGT CTGCGAGGCA AGAATGATCA TCACCGTAGT GAGAGTGCGT
  661 TCAAGGCTCT TGCGGTTGCC ATAAGAGAAG CCACCTCGCC CAATGGTACC AACGATGTTC
  721 CCTCCACCAA AGGTGTTCTT ATGTAGTGAC ACCGATNATT TAAAGCTGCA GCATACGATA
  781 TATATACNTG TGTATATATG TATACCTATG AATGTCAGTA NGTATGTATA CGAACAGTAT
  841 GATACTGAAG ATGANAAGGT AATGCATCAT TCTATACGNG NCANNCNGAA NGANGNGCGC
  901 TTTCCNTTTT TTCNTTTTNG NTTTTTNCNT TTTTTTTTNC NNNNGNNNNC NNCNGNNNNN
961 NNNNN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, Ifa38 5')
Sequence file: RDB16045_A8I3k.seq check
>16045_16045_B0KqF_TDH3pro_F_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT GNNATTCNGT AAATCTATTT CTTAAACTTC TTAAATTCTA
   61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
  121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG
  181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT
  241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG
  301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG
  361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT
  421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA
  481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT
  541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT
  601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT
  661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT
  721 CGGGTACTCG TGGTTTGATC TTAACCATGG GCTCATTTGG TGGTCTGATT CCCACCCCAC
  781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACAAGG TTGGTCTAAC TCTTTGGCTG
  841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTCATATTTG GTCACTANCT
  901 CNANGNCNNA AANCAGAAGA TCATCTTTNA NGATCCNNAA TCCNNNANNN TTTNNNAAAN
961 TCCNNNTTNN ANNNNNNNNN N
//
Primer: Amp_R
Region: Amp pro, CEN_ARS
Sequence file: RDB16045_A8I3l.seq check
>D05803_A9_A8I3_1_Amp_R_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNATTNNNC NNTNATNNGG GTTANTGTCT CNTGANCGGA TACATATTTG
   61 AATGTATTNN NNNNAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT
  181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA
  241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT
  301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT
  361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT
  421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA
  481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT
  541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT
  601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGANNN GGTCACAGCT TGTCTGTAAG
  781 CGGATGCCGG GAGCAGANAA GCCCGTCANG GCGCGTCAGC GGGNNTTGGC GGGNGTCNGG
  841 GGCTGGCTTA ACTATGCGGC ATCAGANCAG ANTGTACTGA GANTGCACCA TAANNTCCCN
901 NTTTAANANC TNGNNNANCG CTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.12.16

GNP_filter3_RDBDEP_html_201216.pl