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Ifa38-FLAG-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Catalog number RDB16045
Resource name Ifa38-FLAG-GFP11
Alternative name pRS313-GPDp-Ifa38-3xFLAG-GFP11 (pSFL86)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS313 (plasmid)
Selectable markers Amp^r (E. coli), HIS3
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae IFA38

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16045 Ifa38-FLAG-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16045zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16045_A8I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro, Ifa38 5')
          Sequence file: RDB16045_A8I3g.seq check
          >16045_16045_B0KqF_HB-NOS_R_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGTNNNGCC NGGGTTTTCC CNGTCACGAC
             61 GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCA
            121 GTTTATCATT ATCAATACTC GCCATTTCAA AGAATACGTA AATAATTAAT AGTAGTGATT
            181 TTCCTAACTT TATTTAGTCA AAAAATTAGC CTTTTAATTC TGCTGTAACC CGTACATGCC
            241 CAAAATAGGG GGCGGGTTAC ACAGAATATA TAACATCGTA GGTGTCTGGG TGAACAGTTT
            301 ATTCCTGGCA TCCACTAAAT ATAATGGAGC CCGCTTTTTA AGCTGGCATC CAGAAAAAAA
            361 AAGAATCCCA GCACCAAAAT ATTGTTTTCT TCACCAACCA TCAGTTCATA GGTCCATTCT
            421 CTTAGCGCAA CTACAGAGAA CAGGGGCACA AACAGGCAAA AAACGGGCAC AACCTCAATG
            481 GAGTGATGCA ACCTGCCTGG AGTAAATGAT GACACAAGGC AATTGACCCA CGCATGTATC
            541 TATCTCATTT TCTTACACCT TCTATTACCT TCTGCTCTCT CTGATTTGGA AAAAGCTGAA
            601 AAAAAAGGTT GAAACCAGTT CCCTGAAATT ATTCCCCTAC TTGACTAATA AGTATATAAA
            661 GACGGTAGGT ATTGATTGTA ATTCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT
            721 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC GGATGCGGCC
            781 GCATGACTTT TATGCAACAG CTTCAAGANG CTGGGGAAAG ATTTANGTGT ATCAATGGTC
            841 TTTTATGGGT TGTTTTTGGG CTTGGTGTCT TAAAATGTAC NACGTTATCA NTTAGATTTT
            901 TTAGCTCTTA NTTTTTGANC TTTTTTTTTA CTANNAGNAN NNNANTTTCN NNNAGTATNG
          961 NTGCTNAAAA NNNGNAAATA CTGNNNNATC ANNGNNNNNN NNNN
          //
          Primer: SV40pA3'element Reverse
          Region: T3 pro, insert 3' (CYC1 ter, 3xFLAG-GFP11 3')
          Sequence file: RDB16045_A8I3h.seq check
          >D05803_A9_A8I3_1_SV40pA3'element Reverse_B04_04_ABI24.ab1
              1 NNNNNNGNNN NNNNNNNNNN NGNNNNNNGC CNGNAACGCG GCCTTTTNAC GGTTCCTGGC
             61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
            121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
            181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
            241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
            301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
            361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
            421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
            481 ACCGGGCCCG GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT
            541 TCAGTATAAT GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA
            601 TAAATAACGT TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG
            661 GTTGTCTAAC TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC
            721 GTGACATAAC TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC
            781 TTATGTTATC CCTGCAGCAT TGACGTATTC ATGCAAAACC ATGTGATCTC NGGAAGTTGA
            841 ACCTCNACCA CTNCCGCCTT TATCGNCATC ATCCTTATAN NCAATGNCAN GATNCTNATA
            901 ATCNCCNTCA NNGNCTTNNN AATCACCNNN GGNNNANGNA NNCNNNNNTT TTTNNNNNNN
          961 NNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC ter, 3xFLAG-GFP11, Ifa38 3')
          Sequence file: RDB16045_A8I3i.seq check
          >D05803_A9_A8I3_1_CYC1ter_R_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA
             61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC
            121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT
            181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG
            241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTCCTTTTTA ACCTGTCTTG CGGCTTTTTT
            301 TAAGGCTCTA ATTCTGATAG ATTTATGGAA GGAATAATTA ATGGAGTTAA CAATCTTAGA
            361 GTAAACGCCA AAGGTCTCTG TAATTACAAA TTGATACACT GCGTGCGCCC AGTAAGGGGT
            421 CATAGTAGCG TATCTTTCTT GAGAGCCGCA GCGTCTGCCA ACGCTTCTTA AAGTGGATTT
            481 TACAAACTGT TGTGGATTTG GGATCATTAA AGATGATCTT CTGATTTTAG ACATCGAGCT
            541 AGTGACCAAA TATGAAATGA TTAATTCAAC ATCGATAGCA TCTTTAGATA ATTCCCCAGC
            601 CAAAGAGTTA GACCAACCTT GTAAGAATGA TTTCGAACCA CTGTATGTAG CCAAAAGTGG
            661 GGTGGGAATC AGANCACCAA ATGAGCCCAT GGTTAAGATC AAACCACGAG TACCCGACTT
            721 CTTGTTTTCA GCTTTCACAG TTTCCACAAT CTTTGGTGCA ATGATTTGTG TAATTAATAA
            781 TGTAGCAGTG TTATTGATAG TGANAATATT TCTAAGCTCC TTCTCTTCTG TNTCCNAAAA
            841 TGNNACANGA ANGGNNNGTG NNNNANNNAN ATTATNGANN NAAANNGGNN NNNNGNANCN
          901 GCGNNCACAA TTCCTNNNNN GNNNTNNNNN NNNNN
          //
          Primer: HIS3pro_F
          Region: HIS3 pro, HIS3
          Sequence file: RDB16045_A8I3j.seq check
          >D05803_A9_A8I3_1_HIS3pro_F_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNTNNNNNNA AGTAANGTGN NTTCTTCGAA NAANATACTA AAAAATGAGC
             61 AGGCAAGATA AACGAAGGCA AAGATGACAG AGCAGAAAGC CCTAGTAAAG CGTATTACAA
            121 ATGAAACCAA GATTCAGATT GCGATCTCTT TAAAGGGTGG TCCCCTAGCG ATAGAGCACT
            181 CGATCTTCCC AGAAAAAGAG GCAGAAGCAG TAGCAGAACA GGCCACACAA TCGCAAGTGA
            241 TTAACGTCCA CACAGGTATA GGGTTTCTGG ACCATATGAT ACATGCTCTG GCCAAGCATT
            301 CCGGCTGGTC GCTAATCGTT GAGTGCATTG GTGACTTACA CATAGACGAC CATCACACCA
            361 CTGAAGACTG CGGGATTGCT CTCGGTCAAG CTTTTAAAGA GGCCCTAGGG GCCGTGCGTG
            421 GAGTAAAAAG GTTTGGATCA GGATTTGCGC CTTTGGATGA GGCACTTTCC AGAGCGGTGG
            481 TAGATCTTTC GAACAGGCCG TACGCAGTTG TCGAACTTGG TTTGCAAAGG GAGAAAGTAG
            541 GAGATCTCTC TTGCGAGATG ATCCCGCATT TTCTTGAAAG CTTTGCAGAG GCTAGCAGAA
            601 TTACCCTCCA CGTTGATTGT CTGCGAGGCA AGAATGATCA TCACCGTAGT GAGAGTGCGT
            661 TCAAGGCTCT TGCGGTTGCC ATAAGAGAAG CCACCTCGCC CAATGGTACC AACGATGTTC
            721 CCTCCACCAA AGGTGTTCTT ATGTAGTGAC ACCGATNATT TAAAGCTGCA GCATACGATA
            781 TATATACNTG TGTATATATG TATACCTATG AATGTCAGTA NGTATGTATA CGAACAGTAT
            841 GATACTGAAG ATGANAAGGT AATGCATCAT TCTATACGNG NCANNCNGAA NGANGNGCGC
            901 TTTCCNTTTT TTCNTTTTNG NTTTTTNCNT TTTTTTTTNC NNNNGNNNNC NNCNGNNNNN
          961 NNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Ifa38 5')
          Sequence file: RDB16045_A8I3k.seq check
          >16045_16045_B0KqF_TDH3pro_F_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT GNNATTCNGT AAATCTATTT CTTAAACTTC TTAAATTCTA
             61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
            121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG
            181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT
            241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG
            301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG
            361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT
            421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA
            481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT
            541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT
            601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT
            661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT
            721 CGGGTACTCG TGGTTTGATC TTAACCATGG GCTCATTTGG TGGTCTGATT CCCACCCCAC
            781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACAAGG TTGGTCTAAC TCTTTGGCTG
            841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTCATATTTG GTCACTANCT
            901 CNANGNCNNA AANCAGAAGA TCATCTTTNA NGATCCNNAA TCCNNNANNN TTTNNNAAAN
          961 TCCNNNTTNN ANNNNNNNNN N
          //
          Primer: Amp_R
          Region: Amp pro, CEN_ARS
          Sequence file: RDB16045_A8I3l.seq check
          >D05803_A9_A8I3_1_Amp_R_D04_10_ABI24.ab1
              1 NNNNNNNNNN NNNATTNNNC NNTNATNNGG GTTANTGTCT CNTGANCGGA TACATATTTG
             61 AATGTATTNN NNNNAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT
            181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA
            241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT
            301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT
            361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT
            421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA
            481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT
            541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT
            601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA
            661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
            721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGANNN GGTCACAGCT TGTCTGTAAG
            781 CGGATGCCGG GAGCAGANAA GCCCGTCANG GCGCGTCAGC GGGNNTTGGC GGGNGTCNGG
            841 GGCTGGCTTA ACTATGCGGC ATCAGANCAG ANTGTACTGA GANTGCACCA TAANNTCCCN
          901 NTTTAANANC TNGNNNANCG CTNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.
          user_report Tasnin, M.N., The vacuole controls nucleolar dynamics and micronucleophagy via the NVJ. Biochem. Biophys. Res. Commun. 550: 158-165 (2021). PMID 33706099.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl