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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Ifa38-GFP11

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP11; location: endoplasmic reticulum.

Catalog number RDB16044
Resource name Ifa38-GFP11
Alternative name pSFL18, pRS314-GPDp-Ifa38-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
S. cerevisiae IFA38

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB16044 Ifa38-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Ifa38-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16044).

          Reference section:

          Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16044_A8I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: TDH3pro_F (Pr0431)
          Region: insert mid(GPD pro,Ifa38 5')
          Sequence file: RDB16044_A8I3e.seq check
          >16044_16044_B3F7F_TDH3pro_F_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTGNNNNNC TGTAAATCTA TTTCTTAAAC TTCTTAAATT
             61 CTACTTTTAT AGNNNNNCNT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT
            121 GCGGCCGCAT GACTTTTATG CAACAGCTTC AAGAGGCTGG GGAAAGATTT AGGTGTATCA
            181 ATGGTCTTTT ATGGGTTGTT TTTGGGCTTG GTGTCTTAAA ATGTACAACG TTATCACTTA
            241 GATTTTTAGC TCTTATTTTT GATCTTTTTT TACTACCAGC AGTCAATTTC GACAAGTATG
            301 GTGCTAAAAC TGGTAAATAC TGTGCTATCA CTGGTGCAAG TGACGGTATT GGTAAAGAAT
            361 TTGCTAGACA AATGGCGAAA CGTGGCTTCA ACTTGGTATT GATCTCGAGA ACGCAATCTA
            421 AATTGGAGGC TTTACAAAAA GAACTAGAAG ATCAACATCA TGTCGTTGTA AAGATTCTAG
            481 CCATTGACAT TGCGGAGGAT AAAGAATCCA ACTATGAATC CATTAAGGAA TTGTGTGCGC
            541 AGTTACCAAT CACCGTTTTG GTCAATAATG TTGGTCAATC ACACTCCATT CCTGTTCCAT
            601 TTTTGGAAAC AGAAGAGAAG GAGCTTAGAA ATATTATCAC TATCAATAAC ACTGCTACAT
            661 TATTAATTAC ACAAATCATT GCACCAAAGA TTGTGGAAAC TGTGAAAGCT GAAAACAAGA
            721 AGTCGGGTAC TCGTGGTTTG ATCTTAACCA TGGGCTCATT TGGTGGTCTG ATTCCCACCC
            781 CACTTTTGGC TACATACAGN GGTTCGAAAT CATTCTTACA AGGTTGGTCT AACTCTTTGG
            841 CTGGGGAATT ATCTAAAGAT GCTATCGATG TTGAATNNAN TCATTNCNTA TTTGGTCNCT
            901 ANCTCGATGN CTAAAATCAN AAGATCATCT TTAATGATNC NNAATNCNCA NCAGTTTGNA
            961 AAATNNNCTT TNANANNCNT NGGNNGANNC TGNNNNTCNN NNNAAANNNN NNCTNNTANN
          1021 NNCCCTTNNN GGGNNNNNNC NNNNNNNNNA NTTTNNNNNN NNNN
          //
          Primer: CYC1ter_R (Pr0022)
          Region: insert mid(CYC1 ter, GFP11,Ifa38 3')
          Sequence file: RDB16044_A8I3f.seq check
          >D05803_A8_A8I3_2_CYC1ter_R_H01_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN CGGNNGTGGG GGGNNGGCGT GAANGTAAGC GTGNCATAAC
             61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTAATC
            121 CCAGCAGCAT TTACGTACTC ATGAAGGACC ATGTGGTCAC GACTTGTTGA TCCGCCACCA
            181 GACCCTCCGG ATCCTTCCTT TTTAACCTGT CTTGCGGCTT TTTTTAAGGC TCTAATTCTG
            241 ATAGATTTAT GGAAGGAATA ATTAATGGAG TTAACAATCT TAGAGTAAAC GCCAAAGGTC
            301 TCTGTAATTA CAAATTGATA CACTGCGTGC GCCCAGTAAG GGGTCATAGT AGCGTATCTT
            361 TCTTGAGAGC CGCAGCGTCT GCCAACGCTT CTTAAAGTGG ATTTTACAAA CTGTTGTGGA
            421 TTTGGGATCA TTAAAGATGA TCTTCTGATT TTAGACATCG AGCTAGTGAC CAAATATGAA
            481 ATGATTAATT CAACATCGAT AGCATCTTTA GATAATTCCC CAGCCAAAGA GTTAGACCAA
            541 CCTTGTAAGA ATGATTTCGA ACCACTGTAT GTAGCCNAAA GTGGGGTGGG AATCAGANCA
            601 CCAAATGAGC CCATGGTTAA GATCAAACCA CGANTACCCG ACTTCTTGTT TTCAGCTTTC
            661 ACAGTTTCCA CAATCTTTGG TGCAATGATT TGTGTAATTA ATAATGTAGC AGNGTTATTG
            721 ATAGTGATAN TATTTCTAAG CTCCTTCTCN TCTGTTTCCN AAAATGGAAC NNNAATGNNN
            781 NGNGNNTGNN CAANNTNATN GANCNAAACG GNGANNNNNN ACNGCNCNCA CNNNTNNNTN
          841 NNNNNNNTNN NNNNNNNN
          //
          Primer: TRP1pro_F (Pr0171)
          Region: TRP1 mid,
          Sequence file: RDB16044_A8I3g.seq check
          >D05803_A8_A8I3_2_TRP1pro_F_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNAA GGCAGCTTGG NNTNNGTCTG TTATTAATTT CNCAGGTAGT
             61 TCTGGTCCAT TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA
            121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC
            181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT
            241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC
            301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA
            361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA
            421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT
            481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC
            541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG
            601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA
            661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATANGT TATTACTGAG
            721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC
            781 AGATGCGTAA GGAGAAAATA CCGCATCAGG AANTTGTAAG CGTTAATATN NTGTTNAAAT
            841 TCGCGTNNAA TTTTTTGTTA AATCAGCTCA TTTTTTTNAN NNATANGCCN AAANCGGNNA
            901 ANNNCNNTNN AANNCNAAAA GANTAGANNN ANNNAGGGNN NNNNGNTNNN NNNNNNTNNG
          961 NNNNNNNNNN
          //
          Primer: SV40pA3element Reverse (Pr0108)
          Region: insert 3'(T3 pro,CYC1 ter)
          Sequence file: RDB16044_A8I3h.seq check
          >D05803_A8_A8I3_2_SV40pA3'element Reverse_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNANG NNNNNGCNNG CAACGCGGCC TTTTNACGGT TCCTGGCCTT
             61 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
            121 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
            181 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
            241 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
            301 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
            361 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA
            421 TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGTACC
            481 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA
            541 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG
            601 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA
            661 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGANGGCG TGAATGTAAG CGTGACATAA
            721 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTATGTAAT
            781 CCCAGCAGCA TTTACGTACT CATGAANGAN CATGNGGTCA CGANTTGTTG ATNNGCCACC
            841 AGANCCNTCC NGNTNCTTCC TTTTTAACCN NNCTNGCGNN TTTTTTTTTN ANNNNNNTNN
          901 NTNNGANNNA NTTNNNNGNA NNNNANNNAN TTNANNNGNN NNTNNNNNAN NNNNNNNNN
          //
          Primer: Amp_R (Pr0008)
          Region: Amp R pro,CEN_ARS
          Sequence file: RDB16044_A8I3i.seq check
          >D05803_A8_A8I3_2_Amp_R_H03_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTGNN GCNNTNATNN GGGTTANTGT CTCATGAGCG GANACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
            181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
            241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
            301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
            361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
            421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
            481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
            541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
            601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
            661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
            721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTNGTCTGTA
            781 ANCGGATGCC GGGANCAGAN AAGCNNGTCA GGGCGCGTCA GCGNNNGTTG GCGGGTGNCN
            841 GGGGCTGGCT NNACTATGCG GCATCAGANC AGANTGTACT GNNANNGCAN CNTANNTCAC
            901 GANNTTACTA TATATATNAN ATAGGNNNNN NTTNNNAGNA NNANCATCNN NNNNNANNNN
          961 NNNNTTNNNN NNNNNN
          //
          Primer: f1ori_R4 (Pr1179)
          Region: insert 5'(T7 pro,GDP pro)
          Sequence file: RDB16044_A8I3j.seq check
          >16044_16044_B3F7F_f1ori_R4_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNANGCGC CNCNNCNGGG CGCGTCCNTT
             61 CGCCNTTCAG GCTGCGCAAC TGTTGGGANN NNNNNNNNNN GCGGGCCTCT TCGCTATTAC
            121 GCCAGCTGGC GAANNNNNNA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT
            181 CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG
            241 AATTGGAGCT CAGTTTATCA TTATCAATAC TCGCCATTTC AAAGAATACG TAAATAATTA
            301 ATAGTAGTGA TTTTCCTAAC TTTATTTAGT CAAAAAATTA GCCTTTTAAT TCTGCTGTAA
            361 CCCGTACATG CCCAAAATAG GGGGCGGGTT ACACAGAATA TATAACATCG TAGGTGTCTG
            421 GGTGAACAGT TTATTCCTGG CATCCACTAA ATATAATGGA GCCCGCTTTT TAAGCTGGCA
            481 TCCAGAAAAA AAAAGAATCC CAGCACCAAA ATATTGTTTT CTTCACCAAC CATCAGTTCA
            541 TAGGTCCATT CTCTTAGCGC AACTACAGAG AACAGGGGCA CAAACAGGCA AAAAACGGGC
            601 ACAACCTCAA TGGAGTGATG CAACCTGCCT GGAGTAAATG ATGACACAAG GCAATTGACC
            661 CACGCATGTA TCTATCTCAT TTTCTTACAC CTTCTATTAC CTTCTGCTCT CTCTGATTTG
            721 GAAAAANCTG AAAAAAAAGG NTGAAACCAG TTCCCTGAAA TTATTCCCCT ACTTGACTAN
            781 NNNNNATATA AAGACGGNAG GNATTGATTG TAATTCNGTA AATCTATTTC NNNAACTTCT
            841 TAAATTCTAC TTTTANAGTT AGNCTTTTTT TTAGTTTTNA AACNCCAGAA CTTANTTTCN
            901 ACGGATGCGN NCNCNTGNNN TTTNNGCNAC AGCTTCNAGA NNNTGGGNAA ANNNTTANGN
            961 GNANCNNNNG NNNTTTTATG GGNNGNTTTN GGNCNNGGNG NNNNNAAANG NNNANNNNNN
          1021 NNNCNNNNNN TTTNNNTNNN NTNNNNNNNT TTTTNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

          2023.06.15

          GNP_filter3_RDBDEP_html_230605.pl