Please review the QC test results indicated by icon below as well as clone information before placing your order.

Ifa38-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16044_A8I3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB16044_A8I3a.seq
>D05803_A8_A8I3_2_M13_-40_F10_16_ABI24.ab1     1 AGAAAGTAAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT    61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA   121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG   181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT   241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA   301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG   361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC   421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC   481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC   541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT   601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG TTAGTCTTTT   661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGA CTTTTATGCA   721 ACAGCTTCAA GAGGCTGGGG AAAGATTTAG GTGTATCAAT GGTCTTTTAT GGGTTGTTTT   781 TGGGCTTGGT GTCTTAAAAT GTACAACGTT ATCACTTAGA TTTTTTAGCT CTTATTTTTT   841 GATCTTTTTT TTTACTACCA GCAGTCAATT TCGACAAGTA TGGTGCTAAA AACTGGGTAA   901 ATACTGTGCT ATCACTGGTG CAAGTGACGG TATTGGTAAA GAAATTTGCT AGACAAATGG   961 GCGAAACGTG GGCTTCCAAC TTGGTATTTG ATCTCGAGAA CGCAAATCTA AATTTGGAGC  1021 TTTACAAAAA GAACTTAGGA GATCCAACAT CCATGTCGCA TGTACGATCT AGACAATTGG 1081 ACATTGGCGA AAGGAT //
Primer: CYC1ter_R Sequence file: RDB16044_A8I3b.seq
>D05803_A8_A8I3_2_CYC1ter_R_H01_22_ABI24.ab1     1 AATTTCATTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC    61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTAATC   121 CCAGCAGCAT TTACGTACTC ATGAAGGACC ATGTGGTCAC GACTTGTTGA TCCGCCACCA   181 GACCCTCCGG ATCCTTCCTT TTTAACCTGT CTTGCGGCTT TTTTTAAGGC TCTAATTCTG   241 ATAGATTTAT GGAAGGAATA ATTAATGGAG TTAACAATCT TAGAGTAAAC GCCAAAGGTC   301 TCTGTAATTA CAAATTGATA CACTGCGTGC GCCCAGTAAG GGGTCATAGT AGCGTATCTT   361 TCTTGAGAGC CGCAGCGTCT GCCAACGCTT CTTAAAGTGG ATTTTACAAA CTGTTGTGGA   421 TTTGGGATCA TTAAAGATGA TCTTCTGATT TTAGACATCG AGCTAGTGAC CAAATATGAA   481 ATGATTAATT CAACATCGAT AGCATCTTTA GATAATTCCC CAGCCAAAGA GTTAGACCAA   541 CCTTGTAAGA ATGATTTCGA ACCACTGTAT GTAGCCAAAA GTGGGGTGGG AATCAGACCA   601 CCAAATGAGC CCATGGTTAA GATCAAACCA CGAGTACCCG ACTTCTTGTT TTCAGCTTTC   661 ACAGTTTCCA CAATCTTTGG TGCAATGATT TGTGTAATTA ATAATGTAGC AGTGTTATTG   721 ATAGTGATAA TATTTCTAAG CTCCTTCTCT TCTGTTTCCA AAAATGGAAC AGGAATGGAG   781 TGTGATTGAC CAACATTATT GACCAAAACG GTGATTGGTA ACTGCGCACA CAATTCCTTA   841 ATGGAATTCA TAGTTGGATT CTTTATCCTC CGGCAATGTC AATGGCTAGA AATCTTTACA   901 ACGACATGAT GTTTGATCTT CTAGTTCTTT TTTGTAAAGC CTCCAATTAG ATTGCGTTTC   961 TCGAGATCAA TACCCAAGCT GGAAGGCCAC GTTTCGTCAT TGTCCTAGCA ATTCTTACCA 1021 TACGTCACTT GCACAGTGAT AGCACAGATA TTTAGCCAAG TTATAC //
Primer: TRP1pro_F Sequence file: RDB16044_A8I3c.seq
>D05803_A8_A8I3_2_TRP1pro_F_G03_21_ABI24.ab1     1 TCTGGTAATA GGCACACAAA GGCAGCTTGG AGTATGTCTG TTATTAATTT CACAGGTAGT    61 TCTGGTCCAT TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA   121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC   181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT   241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC   301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA   361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA   421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT   481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC   541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG   601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA   661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG   721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC   781 AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAG CGTTAATATT TTGTTAAAAT   841 TCGCGTTAAA TTTTTTGTTA AATCAGCTCA TTTTTTTAAC CAATAGGCCG AAATCGGCAA   901 AATCCCTTAT AAATCAAAAA GAATAGACCG AGATAGGGTT GAGTGTTTGT TCCAGTTTGG   961 GAACAAGAGT CCACTATTTA AAGAACGTGC ACTCCAACGT CAAAGGCGAA AAAACGTCTA  1021 TCAGACGATG GACCAACTAC GTGGATCATC ACCGTATCAG TTTTTTGGGT TCGAGAGCCG 1081 TTAAAAGC //
Primer: Amp_R Sequence file: RDB16044_A8I3d.seq
>D05803_A8_A8I3_2_Amp_R_H03_24_ABI24.ab1     1 TCATTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA   181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT   241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT   301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG   361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC   421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT   481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT   541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT   601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT   661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG   721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA   781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG   841 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATAGATCAC   901 GACATTACTA TATATATAAT ATAGGAAGCA TTTAATAGAA ACAGCATCGT AATATATGTG   961 TACTTTTGCA GTTATGACGC CAGATGGGCA GTAGTGGAGA TATTCTTTTA TTGAAAAATA  1021 GCTTGTCACC TTACGTACAT CCTGAATCGG AGCTTTCGTT TTTTTGCGGA TAAGGAGTTT 1081 AGTTCCGGTC GCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl