Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Ifa38-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Catalog number RDB16044
Resource name Ifa38-GFP11
Alternative name pRS314-GPDp-Ifa38-GFP11 (pSFL18)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae IFA38

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16044 Ifa38-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16044_A8I3p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB16044_A8I3a.seq check
          >D05803_A8_A8I3_2_M13_-40_F10_16_ABI24.ab1
              1 AGAAAGTAAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT
             61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA
            121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG
            181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT
            241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA
            301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG
            361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC
            421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC
            481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC
            541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT
            601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG TTAGTCTTTT
            661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGA CTTTTATGCA
            721 ACAGCTTCAA GAGGCTGGGG AAAGATTTAG GTGTATCAAT GGTCTTTTAT GGGTTGTTTT
            781 TGGGCTTGGT GTCTTAAAAT GTACAACGTT ATCACTTAGA TTTTTTAGCT CTTATTTTTT
            841 GATCTTTTTT TTTACTACCA GCAGTCAATT TCGACAAGTA TGGTGCTAAA AACTGGGTAA
            901 ATACTGTGCT ATCACTGGTG CAAGTGACGG TATTGGTAAA GAAATTTGCT AGACAAATGG
            961 GCGAAACGTG GGCTTCCAAC TTGGTATTTG ATCTCGAGAA CGCAAATCTA AATTTGGAGC
           1021 TTTACAAAAA GAACTTAGGA GATCCAACAT CCATGTCGCA TGTACGATCT AGACAATTGG
          1081 ACATTGGCGA AAGGAT
          //
          Primer: CYC1ter_R
          Sequence file: RDB16044_A8I3b.seq check
          >D05803_A8_A8I3_2_CYC1ter_R_H01_22_ABI24.ab1
              1 AATTTCATTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC
             61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTAATC
            121 CCAGCAGCAT TTACGTACTC ATGAAGGACC ATGTGGTCAC GACTTGTTGA TCCGCCACCA
            181 GACCCTCCGG ATCCTTCCTT TTTAACCTGT CTTGCGGCTT TTTTTAAGGC TCTAATTCTG
            241 ATAGATTTAT GGAAGGAATA ATTAATGGAG TTAACAATCT TAGAGTAAAC GCCAAAGGTC
            301 TCTGTAATTA CAAATTGATA CACTGCGTGC GCCCAGTAAG GGGTCATAGT AGCGTATCTT
            361 TCTTGAGAGC CGCAGCGTCT GCCAACGCTT CTTAAAGTGG ATTTTACAAA CTGTTGTGGA
            421 TTTGGGATCA TTAAAGATGA TCTTCTGATT TTAGACATCG AGCTAGTGAC CAAATATGAA
            481 ATGATTAATT CAACATCGAT AGCATCTTTA GATAATTCCC CAGCCAAAGA GTTAGACCAA
            541 CCTTGTAAGA ATGATTTCGA ACCACTGTAT GTAGCCAAAA GTGGGGTGGG AATCAGACCA
            601 CCAAATGAGC CCATGGTTAA GATCAAACCA CGAGTACCCG ACTTCTTGTT TTCAGCTTTC
            661 ACAGTTTCCA CAATCTTTGG TGCAATGATT TGTGTAATTA ATAATGTAGC AGTGTTATTG
            721 ATAGTGATAA TATTTCTAAG CTCCTTCTCT TCTGTTTCCA AAAATGGAAC AGGAATGGAG
            781 TGTGATTGAC CAACATTATT GACCAAAACG GTGATTGGTA ACTGCGCACA CAATTCCTTA
            841 ATGGAATTCA TAGTTGGATT CTTTATCCTC CGGCAATGTC AATGGCTAGA AATCTTTACA
            901 ACGACATGAT GTTTGATCTT CTAGTTCTTT TTTGTAAAGC CTCCAATTAG ATTGCGTTTC
            961 TCGAGATCAA TACCCAAGCT GGAAGGCCAC GTTTCGTCAT TGTCCTAGCA ATTCTTACCA
          1021 TACGTCACTT GCACAGTGAT AGCACAGATA TTTAGCCAAG TTATAC
          //
          Primer: TRP1pro_F
          Sequence file: RDB16044_A8I3c.seq check
          >D05803_A8_A8I3_2_TRP1pro_F_G03_21_ABI24.ab1
              1 TCTGGTAATA GGCACACAAA GGCAGCTTGG AGTATGTCTG TTATTAATTT CACAGGTAGT
             61 TCTGGTCCAT TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA
            121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC
            181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT
            241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC
            301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA
            361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA
            421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT
            481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC
            541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG
            601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA
            661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG
            721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC
            781 AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAG CGTTAATATT TTGTTAAAAT
            841 TCGCGTTAAA TTTTTTGTTA AATCAGCTCA TTTTTTTAAC CAATAGGCCG AAATCGGCAA
            901 AATCCCTTAT AAATCAAAAA GAATAGACCG AGATAGGGTT GAGTGTTTGT TCCAGTTTGG
            961 GAACAAGAGT CCACTATTTA AAGAACGTGC ACTCCAACGT CAAAGGCGAA AAAACGTCTA
           1021 TCAGACGATG GACCAACTAC GTGGATCATC ACCGTATCAG TTTTTTGGGT TCGAGAGCCG
          1081 TTAAAAGC
          //
          Primer: Amp_R
          Sequence file: RDB16044_A8I3d.seq check
          >D05803_A8_A8I3_2_Amp_R_H03_24_ABI24.ab1
              1 TCATTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
            181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
            241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
            301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
            361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
            421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
            481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
            541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
            601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
            661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
            721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA
            781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG
            841 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATAGATCAC
            901 GACATTACTA TATATATAAT ATAGGAAGCA TTTAATAGAA ACAGCATCGT AATATATGTG
            961 TACTTTTGCA GTTATGACGC CAGATGGGCA GTAGTGGAGA TATTCTTTTA TTGAAAAATA
           1021 GCTTGTCACC TTACGTACAT CCTGAATCGG AGCTTTCGTT TTTTTGCGGA TAAGGAGTTT
          1081 AGTTCCGGTC GCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl