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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Ifa38-GFP11 (#RDB16044)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP11; location: endoplasmic reticulum.

Alternative name pSFL18, pRS314-GPDp-Ifa38-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor|Developer Tamura, Yasushi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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必要書類
提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

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RDB16044 Ifa38-GFP11 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Ifa38-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16044).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16044_A8I3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: TDH3pro_F (Pr0431)
Region: insert mid(GPD pro,Ifa38 5')
Sequence file: RDB16044_A8I3e.seq check
>16044_16044_B3F7F_TDH3pro_F_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTGNNNNNC TGTAAATCTA TTTCTTAAAC TTCTTAAATT
   61 CTACTTTTAT AGNNNNNCNT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT
  121 GCGGCCGCAT GACTTTTATG CAACAGCTTC AAGAGGCTGG GGAAAGATTT AGGTGTATCA
  181 ATGGTCTTTT ATGGGTTGTT TTTGGGCTTG GTGTCTTAAA ATGTACAACG TTATCACTTA
  241 GATTTTTAGC TCTTATTTTT GATCTTTTTT TACTACCAGC AGTCAATTTC GACAAGTATG
  301 GTGCTAAAAC TGGTAAATAC TGTGCTATCA CTGGTGCAAG TGACGGTATT GGTAAAGAAT
  361 TTGCTAGACA AATGGCGAAA CGTGGCTTCA ACTTGGTATT GATCTCGAGA ACGCAATCTA
  421 AATTGGAGGC TTTACAAAAA GAACTAGAAG ATCAACATCA TGTCGTTGTA AAGATTCTAG
  481 CCATTGACAT TGCGGAGGAT AAAGAATCCA ACTATGAATC CATTAAGGAA TTGTGTGCGC
  541 AGTTACCAAT CACCGTTTTG GTCAATAATG TTGGTCAATC ACACTCCATT CCTGTTCCAT
  601 TTTTGGAAAC AGAAGAGAAG GAGCTTAGAA ATATTATCAC TATCAATAAC ACTGCTACAT
  661 TATTAATTAC ACAAATCATT GCACCAAAGA TTGTGGAAAC TGTGAAAGCT GAAAACAAGA
  721 AGTCGGGTAC TCGTGGTTTG ATCTTAACCA TGGGCTCATT TGGTGGTCTG ATTCCCACCC
  781 CACTTTTGGC TACATACAGN GGTTCGAAAT CATTCTTACA AGGTTGGTCT AACTCTTTGG
  841 CTGGGGAATT ATCTAAAGAT GCTATCGATG TTGAATNNAN TCATTNCNTA TTTGGTCNCT
  901 ANCTCGATGN CTAAAATCAN AAGATCATCT TTAATGATNC NNAATNCNCA NCAGTTTGNA
  961 AAATNNNCTT TNANANNCNT NGGNNGANNC TGNNNNTCNN NNNAAANNNN NNCTNNTANN
1021 NNCCCTTNNN GGGNNNNNNC NNNNNNNNNA NTTTNNNNNN NNNN
//
Primer: CYC1ter_R (Pr0022)
Region: insert mid(CYC1 ter, GFP11,Ifa38 3')
Sequence file: RDB16044_A8I3f.seq check
>D05803_A8_A8I3_2_CYC1ter_R_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CGGNNGTGGG GGGNNGGCGT GAANGTAAGC GTGNCATAAC
   61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTAATC
  121 CCAGCAGCAT TTACGTACTC ATGAAGGACC ATGTGGTCAC GACTTGTTGA TCCGCCACCA
  181 GACCCTCCGG ATCCTTCCTT TTTAACCTGT CTTGCGGCTT TTTTTAAGGC TCTAATTCTG
  241 ATAGATTTAT GGAAGGAATA ATTAATGGAG TTAACAATCT TAGAGTAAAC GCCAAAGGTC
  301 TCTGTAATTA CAAATTGATA CACTGCGTGC GCCCAGTAAG GGGTCATAGT AGCGTATCTT
  361 TCTTGAGAGC CGCAGCGTCT GCCAACGCTT CTTAAAGTGG ATTTTACAAA CTGTTGTGGA
  421 TTTGGGATCA TTAAAGATGA TCTTCTGATT TTAGACATCG AGCTAGTGAC CAAATATGAA
  481 ATGATTAATT CAACATCGAT AGCATCTTTA GATAATTCCC CAGCCAAAGA GTTAGACCAA
  541 CCTTGTAAGA ATGATTTCGA ACCACTGTAT GTAGCCNAAA GTGGGGTGGG AATCAGANCA
  601 CCAAATGAGC CCATGGTTAA GATCAAACCA CGANTACCCG ACTTCTTGTT TTCAGCTTTC
  661 ACAGTTTCCA CAATCTTTGG TGCAATGATT TGTGTAATTA ATAATGTAGC AGNGTTATTG
  721 ATAGTGATAN TATTTCTAAG CTCCTTCTCN TCTGTTTCCN AAAATGGAAC NNNAATGNNN
  781 NGNGNNTGNN CAANNTNATN GANCNAAACG GNGANNNNNN ACNGCNCNCA CNNNTNNNTN
841 NNNNNNNTNN NNNNNNNN
//
Primer: TRP1pro_F (Pr0171)
Region: TRP1 mid,
Sequence file: RDB16044_A8I3g.seq check
>D05803_A8_A8I3_2_TRP1pro_F_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAA GGCAGCTTGG NNTNNGTCTG TTATTAATTT CNCAGGTAGT
   61 TCTGGTCCAT TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA
  121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC
  181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT
  241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC
  301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA
  361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA
  421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT
  481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC
  541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG
  601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA
  661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATANGT TATTACTGAG
  721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC
  781 AGATGCGTAA GGAGAAAATA CCGCATCAGG AANTTGTAAG CGTTAATATN NTGTTNAAAT
  841 TCGCGTNNAA TTTTTTGTTA AATCAGCTCA TTTTTTTNAN NNATANGCCN AAANCGGNNA
  901 ANNNCNNTNN AANNCNAAAA GANTAGANNN ANNNAGGGNN NNNNGNTNNN NNNNNNTNNG
961 NNNNNNNNNN
//
Primer: SV40pA3element Reverse (Pr0108)
Region: insert 3'(T3 pro,CYC1 ter)
Sequence file: RDB16044_A8I3h.seq check
>D05803_A8_A8I3_2_SV40pA3'element Reverse_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNANG NNNNNGCNNG CAACGCGGCC TTTTNACGGT TCCTGGCCTT
   61 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
  121 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
  181 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
  241 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
  301 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
  361 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA
  421 TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGTACC
  481 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA
  541 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG
  601 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA
  661 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGANGGCG TGAATGTAAG CGTGACATAA
  721 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTATGTAAT
  781 CCCAGCAGCA TTTACGTACT CATGAANGAN CATGNGGTCA CGANTTGTTG ATNNGCCACC
  841 AGANCCNTCC NGNTNCTTCC TTTTTAACCN NNCTNGCGNN TTTTTTTTTN ANNNNNNTNN
901 NTNNGANNNA NTTNNNNGNA NNNNANNNAN TTNANNNGNN NNTNNNNNAN NNNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: Amp R pro,CEN_ARS
Sequence file: RDB16044_A8I3i.seq check
>D05803_A8_A8I3_2_Amp_R_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTGNN GCNNTNATNN GGGTTANTGT CTCATGAGCG GANACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
  181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
  241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
  301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
  361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
  421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
  481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
  541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
  601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
  661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
  721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTNGTCTGTA
  781 ANCGGATGCC GGGANCAGAN AAGCNNGTCA GGGCGCGTCA GCGNNNGTTG GCGGGTGNCN
  841 GGGGCTGGCT NNACTATGCG GCATCAGANC AGANTGTACT GNNANNGCAN CNTANNTCAC
  901 GANNTTACTA TATATATNAN ATAGGNNNNN NTTNNNAGNA NNANCATCNN NNNNNANNNN
961 NNNNTTNNNN NNNNNN
//
Primer: f1ori_R4 (Pr1179)
Region: insert 5'(T7 pro,GDP pro)
Sequence file: RDB16044_A8I3j.seq check
>16044_16044_B3F7F_f1ori_R4_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNANGCGC CNCNNCNGGG CGCGTCCNTT
   61 CGCCNTTCAG GCTGCGCAAC TGTTGGGANN NNNNNNNNNN GCGGGCCTCT TCGCTATTAC
  121 GCCAGCTGGC GAANNNNNNA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT
  181 CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG
  241 AATTGGAGCT CAGTTTATCA TTATCAATAC TCGCCATTTC AAAGAATACG TAAATAATTA
  301 ATAGTAGTGA TTTTCCTAAC TTTATTTAGT CAAAAAATTA GCCTTTTAAT TCTGCTGTAA
  361 CCCGTACATG CCCAAAATAG GGGGCGGGTT ACACAGAATA TATAACATCG TAGGTGTCTG
  421 GGTGAACAGT TTATTCCTGG CATCCACTAA ATATAATGGA GCCCGCTTTT TAAGCTGGCA
  481 TCCAGAAAAA AAAAGAATCC CAGCACCAAA ATATTGTTTT CTTCACCAAC CATCAGTTCA
  541 TAGGTCCATT CTCTTAGCGC AACTACAGAG AACAGGGGCA CAAACAGGCA AAAAACGGGC
  601 ACAACCTCAA TGGAGTGATG CAACCTGCCT GGAGTAAATG ATGACACAAG GCAATTGACC
  661 CACGCATGTA TCTATCTCAT TTTCTTACAC CTTCTATTAC CTTCTGCTCT CTCTGATTTG
  721 GAAAAANCTG AAAAAAAAGG NTGAAACCAG TTCCCTGAAA TTATTCCCCT ACTTGACTAN
  781 NNNNNATATA AAGACGGNAG GNATTGATTG TAATTCNGTA AATCTATTTC NNNAACTTCT
  841 TAAATTCTAC TTTTANAGTT AGNCTTTTTT TTAGTTTTNA AACNCCAGAA CTTANTTTCN
  901 ACGGATGCGN NCNCNTGNNN TTTNNGCNAC AGCTTCNAGA NNNTGGGNAA ANNNTTANGN
  961 GNANCNNNNG NNNTTTTATG GGNNGNTTTN GGNCNNGGNG NNNNNAAANG NNNANNNNNN
1021 NNNCNNNNNN TTTNNNTNNN NTNNNNNNNT TTTTNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]