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Ifa38-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Catalog number RDB16044
Resource name Ifa38-GFP11
Alternative name pRS314-GPDp-Ifa38-GFP11 (pSFL18)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae IFA38 (KEGG orthology K10251) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB16044 Ifa38-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16044_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB16044_A8I3a.seq check
>D05803_A8_A8I3_2_M13_-40_F10_16_ABI24.ab1
    1 AGAAAGTAAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT
   61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA
  121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG
  181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT
  241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA
  301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG
  361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC
  421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC
  481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC
  541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT
  601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG TTAGTCTTTT
  661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGA CTTTTATGCA
  721 ACAGCTTCAA GAGGCTGGGG AAAGATTTAG GTGTATCAAT GGTCTTTTAT GGGTTGTTTT
  781 TGGGCTTGGT GTCTTAAAAT GTACAACGTT ATCACTTAGA TTTTTTAGCT CTTATTTTTT
  841 GATCTTTTTT TTTACTACCA GCAGTCAATT TCGACAAGTA TGGTGCTAAA AACTGGGTAA
  901 ATACTGTGCT ATCACTGGTG CAAGTGACGG TATTGGTAAA GAAATTTGCT AGACAAATGG
  961 GCGAAACGTG GGCTTCCAAC TTGGTATTTG ATCTCGAGAA CGCAAATCTA AATTTGGAGC
 1021 TTTACAAAAA GAACTTAGGA GATCCAACAT CCATGTCGCA TGTACGATCT AGACAATTGG
1081 ACATTGGCGA AAGGAT
//
Primer: CYC1ter_R
Sequence file: RDB16044_A8I3b.seq check
>D05803_A8_A8I3_2_CYC1ter_R_H01_22_ABI24.ab1
    1 AATTTCATTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC
   61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTAATC
  121 CCAGCAGCAT TTACGTACTC ATGAAGGACC ATGTGGTCAC GACTTGTTGA TCCGCCACCA
  181 GACCCTCCGG ATCCTTCCTT TTTAACCTGT CTTGCGGCTT TTTTTAAGGC TCTAATTCTG
  241 ATAGATTTAT GGAAGGAATA ATTAATGGAG TTAACAATCT TAGAGTAAAC GCCAAAGGTC
  301 TCTGTAATTA CAAATTGATA CACTGCGTGC GCCCAGTAAG GGGTCATAGT AGCGTATCTT
  361 TCTTGAGAGC CGCAGCGTCT GCCAACGCTT CTTAAAGTGG ATTTTACAAA CTGTTGTGGA
  421 TTTGGGATCA TTAAAGATGA TCTTCTGATT TTAGACATCG AGCTAGTGAC CAAATATGAA
  481 ATGATTAATT CAACATCGAT AGCATCTTTA GATAATTCCC CAGCCAAAGA GTTAGACCAA
  541 CCTTGTAAGA ATGATTTCGA ACCACTGTAT GTAGCCAAAA GTGGGGTGGG AATCAGACCA
  601 CCAAATGAGC CCATGGTTAA GATCAAACCA CGAGTACCCG ACTTCTTGTT TTCAGCTTTC
  661 ACAGTTTCCA CAATCTTTGG TGCAATGATT TGTGTAATTA ATAATGTAGC AGTGTTATTG
  721 ATAGTGATAA TATTTCTAAG CTCCTTCTCT TCTGTTTCCA AAAATGGAAC AGGAATGGAG
  781 TGTGATTGAC CAACATTATT GACCAAAACG GTGATTGGTA ACTGCGCACA CAATTCCTTA
  841 ATGGAATTCA TAGTTGGATT CTTTATCCTC CGGCAATGTC AATGGCTAGA AATCTTTACA
  901 ACGACATGAT GTTTGATCTT CTAGTTCTTT TTTGTAAAGC CTCCAATTAG ATTGCGTTTC
  961 TCGAGATCAA TACCCAAGCT GGAAGGCCAC GTTTCGTCAT TGTCCTAGCA ATTCTTACCA
1021 TACGTCACTT GCACAGTGAT AGCACAGATA TTTAGCCAAG TTATAC
//
Primer: TRP1pro_F
Sequence file: RDB16044_A8I3c.seq check
>D05803_A8_A8I3_2_TRP1pro_F_G03_21_ABI24.ab1
    1 TCTGGTAATA GGCACACAAA GGCAGCTTGG AGTATGTCTG TTATTAATTT CACAGGTAGT
   61 TCTGGTCCAT TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA
  121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC
  181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT
  241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC
  301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA
  361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA
  421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT
  481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC
  541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG
  601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA
  661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG
  721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC
  781 AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAG CGTTAATATT TTGTTAAAAT
  841 TCGCGTTAAA TTTTTTGTTA AATCAGCTCA TTTTTTTAAC CAATAGGCCG AAATCGGCAA
  901 AATCCCTTAT AAATCAAAAA GAATAGACCG AGATAGGGTT GAGTGTTTGT TCCAGTTTGG
  961 GAACAAGAGT CCACTATTTA AAGAACGTGC ACTCCAACGT CAAAGGCGAA AAAACGTCTA
 1021 TCAGACGATG GACCAACTAC GTGGATCATC ACCGTATCAG TTTTTTGGGT TCGAGAGCCG
1081 TTAAAAGC
//
Primer: Amp_R
Sequence file: RDB16044_A8I3d.seq check
>D05803_A8_A8I3_2_Amp_R_H03_24_ABI24.ab1
    1 TCATTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
  181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
  241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
  301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
  361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
  421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
  481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
  541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
  601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
  661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
  721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA
  781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG
  841 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATAGATCAC
  901 GACATTACTA TATATATAAT ATAGGAAGCA TTTAATAGAA ACAGCATCGT AATATATGTG
  961 TACTTTTGCA GTTATGACGC CAGATGGGCA GTAGTGGAGA TATTCTTTTA TTGAAAAATA
 1021 GCTTGTCACC TTACGTACAT CCTGAATCGG AGCTTTCGTT TTTTTGCGGA TAAGGAGTTT
1081 AGTTCCGGTC GCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl