Please review the QC test results indicated by icon below as well as clone information before placing your order.

Ifa38-GFP1-10

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering	Please visit Information of Request for Distribution.  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16043_A8I3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Region: GPD pro,insert5' Sequence file: RDB16043_A8I3a.seq
>D05803_A7_A8I3_1_M13_-40_E10_13_ABI24.ab1     1 AACCACTAAT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA TCATTATCAA    61 TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT AACTTTATTT   121 AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA TAGGGGGCGG   181 GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC TGGCATCCAC   241 TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA TCCCAGCACC   301 AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG CGCAACTACA   361 GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG ATGCAACCTG   421 CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT CATTTTCTTA   481 CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA AGGTTGAAAC   541 CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG TAGGTATTGA   601 TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA GTTAGTCTTT   661 TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCATG ACTTTTATGC   721 AACAGCTTCA AGAGGCTGGG GAAAGATTTA GGTGTATCAA TGGTCTTTTA TGGGTTGTTT   781 TTGGGCTTGG TGTCTTAAAA TGTACAACGT TATCACTTAG ATTTTTAGCT CTTATTTTTG   841 ATCTTTTTTT ACTACCAGCA GTCAATTTCG ACAAGTATGG TGCTAAAAAC TGGTAAATAC   901 TGTGCTATCA CTGGTGCAAG TGACGGTATT GGGTAAAAGA AATTTGCTAG ACAAATGGCG   961 AAACGTGGCT TCCAACTTGG GTATTTGATC TCGAGGAACG CAATCTAAAT TTGTGGCTTT  1021 ACAAAAGAAC TAGAAGATCC ACCATCAATG GTTCGTGTAA AGAATTCTAA GTCAATGACA 1081 TTGGCTGCAG GCAATTAAAG GCAT //
Primer: CYC1ter_R Region: CYC1 ter,insert3' Sequence file: RDB16043_A8I3b.seq
>D05803_A7_A8I3_1_CYC1ter_R_G01_19_ABI24.ab1     1 ATTTCGTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT    61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT   121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG   181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT   241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA   301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT   361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA   421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT   481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG   541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT   601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT   661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA   721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC   781 CAGACCCTCC GGATCCTTCC TTTTTAACCT GTCTTGCGGC TTTTTTTAAA GGCTCTAATT   841 CTGATAGATT TATGGAAGGA AATAATTAAT GGAGTTAACA ATCTTAGAGT AAACGCCCAA   901 AGGTCTCTGT AATTACAAAT TTGATACACT GCGTGCGCCC AGTAAAGGGT CATAGTAGCG   961 TATCTTTTCT TGAGAGCCGC AGCCGTCTGC GACGCTTCTA AGTGATTTTA CAACTGTTGT 1021 GAATTGGAAT CATGAGATGA TCTTCTGATT TTTAGAACAT CGAAGCT //
Primer: URA3pro_F Region: URA3pro,URA3 Sequence file: RDB16043_A8I3c.seq
>D05803_A7_A8I3_1_URA3pro_F_C03_09_ABI24.ab1     1 CAAGGCTAGT GTTGAGAAAC ATGAATTGCC CAGTATTCTT AACCCAACTG CACAGAACAA    61 AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA   121 CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT   181 TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG   241 GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG   301 GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA   361 GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA   421 TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG   481 GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG ATGTTAGCAG   541 AATTGTCATG CAAGGGCTCC CTATCTACTG GAGAATATAC TAAGGGTACT GTTGACATTG   601 CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG GGTGGAAGAG   661 ATGAAGGTTA CGATTGGTTG ATTATGACAC CCGGTGTGGG TTTAGATGAC AAGGGAGACG   721 CATTGGGTCA ACAGTATAGA ACCGTGGATG ATGTGGTCTC TACAGGATCT GACATTATTA   781 TTGTTGGAAG AGGACTATTT GCAAAGGGAA GGGATGCTAA GGTAGAGGGT GAACGTTACA   841 GAAAAGCAGG CTGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA   901 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG   961 TTTATTACCC TATGCGGTGT GAAATACCGC ACAGATGCGT AAAGGAGAAA AATACCGCAT  1021 CAGGAAATTT GTAAGCGTTA ATATTTTGAT AAAATTCGCG TCAATTTTTG GTAAATCAGC 1081 TCATTTTTTT AACCA //
Primer: Amp_R Region: AmpR pro,CEN_ARS Sequence file: RDB16043_A8I3d.seq
>D05803_A7_A8I3_1_Amp_R_D03_12_ABI24.ab1     1 TCCGTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA   181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT   241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT   301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG   361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC   421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT   481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT   541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT   601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT   661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG   721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA   781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG   841 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATACCACCT   901 TTTTCAATTC ATCATTTTTT TTTTTATTCT TTTTTTTTTG ATTTCGGTTT CCCTTGAAAT   961 TTTTTTTTGA TTTCGGTAAT CTCCCGAACA GAACGAAGAC GAACGGATGG AGCACAGAAC  1021 TTTAGAATTT GGTATATATA CGCATATTGT AGTGGATGGA GGAAACATGC AATGCCAGTA 1081 TTCCTAACCC AACCTGCACA GGAAACA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl