Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Ifa38-GFP1-10

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: endoplasmic reticulum.

Catalog number RDB16043
Resource name Ifa38-GFP1-10
Alternative name pSFL16, pRS316-GPDp-Ifa38-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae IFA38

          Reference sequence
            
           
          Sequence (full) RDB16043zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16043 Ifa38-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16043_A8I3p1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro)
          Sequence file: RDB16043_A8I3i.seq check
          >16043_16043_B0JcF_HB-NOS_R_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG GTAACGCCNG GGTTTTCCNN GTCACGACGT
             61 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCAGT
            121 TTATCATTAT CAATACTCGC CATTTCAAAG AATACGTAAA TAATTAATAG TAGTGATTTT
            181 CCTAACTTTA TTTAGTCAAA AAATTAGCCT TTTAATTCTG CTGTAACCCG TACATGCCCA
            241 AAATAGGGGG CGGGTTACAC AGAATATATA ACATCGTAGG TGTCTGGGTG AACAGTTTAT
            301 TCCTGGCATC CACTAAATAT AATGGAGCCC GCTTTTTAAG CTGGCATCCA GAAAAAAAAA
            361 GAATCCCAGC ACCAAAATAT TGTTTTCTTC ACCAACCATC AGTTCATAGG TCCATTCTCT
            421 TAGCGCAACT ACAGAGAACA GGGGCACAAA CAGGCAAAAA ACGGGCACAA CCTCAATGGA
            481 GTGATGCAAC CTGCCTGGAG TAAATGATGA CACAAGGCAA TTGANCCACG CATGTATCTA
            541 TCTCATTTTC TTACNNNTTC TATTACCTTC NGCTCTCTCT GATTTGGAAA AAGCTGAAAA
            601 AAAANGNTGN NNCANTTCCC TGAAANTATT CCCCTACTNN ACTNNNANNA NNNNANNANN
          661 NNANGNANNN NNNNNNN
          //
          Primer: SV40pA3'Element Reverse
          Region: T3 pro, insert 3' (CYC1 ter)
          Sequence file: RDB16043_A8I3j.seq check
          >16043_16043_B0JcF_SV40pA3'elementReverse_A02_02_ABI24.ab1
              1 NNNNNNNNGN NNNNNNNNNA NGNNNNNNCN NGCAACGCGG CCTTTTTACG GTTCCTGGCC
             61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
            121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
            181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
            241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
            301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG
            361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC
            421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA
            481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT
            541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA
            601 CGTTCTTAAT ACTAACATAA CTATNAAAAA AATAAATANG GANCTANACT TCNNNNTGTC
            661 NNANTCCNTC CNTTTTNNNN NANNNCNNNN GTNNNNGNNG NNNNGNANNG NNNNCNNNNN
          721 NNNNN
          //
          Primer: paGFP-M_R1
          Region: insert mid (GFP1-10 5', Ifa38 3')
          Sequence file: RDB16043_A8I3k.seq check
          >16043_16043_B0JcF_paGFP-M_R1_G01_19_ABI24.ab1
              1 NNNNNNNNNN NAANNNNTGC CTTTTNNTGT GNTCCGGANA ACGGGAAAAG CATTGAACAC
             61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA
            121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA
            181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA
            241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC
            301 CTTCCTTTTT AACCTGTCTT GCGGCTTTTT TTAAGGCTCT AATTCTGATA GATTTATGGA
            361 AGGAATAATT AATGGAGTTA ACAATCTTAG AGTAAACGCC AAAGGTCTCT GTAATTACAA
            421 ATTGATACAC TGCGTGCGCC CAGTAAGGGG TCATAGTAGC GTATCTTTCT TGAGAGCCGC
            481 AGCGTCTGCC AACGCTTCTT AAAGTGGATT TTACAAACTG TTGTGGATTT GGGATCATTA
            541 AAGATGATCT TCTGATTTTA GACATCGAGC TAGTGACCAA ATATGAAATG ATTAATTCAA
            601 CATCGATAGC ATCTTTAGAT AATTCCCCAG CCAAAGAGTT AGACCAACCT TGTAAGANNG
            661 ANTTCNAACC NCTGTATGTA GNCNNAAAGT GGGGNGGGAA TCNNANCACC AAATGANCCC
          721 NNNGNTNAAN NNCAAANNNN NANNNNCNNN NN
          //
          Primer: URA3pro_F
          Region: URA3 pro, URA3
          Sequence file: RDB16043_A8I3p.seq check
          >D05803_A7_A8I3_1_URA3pro_F_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTGANNTGCC NNGTATTCTT AACCCAACTG CACAGAACAA
             61 AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA
            121 CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT
            181 TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG
            241 GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG
            301 GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA
            361 GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA
            421 TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG
            481 GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG ATGTTAGCAG
            541 AATTGTCATG CAAGGGCTCC CTATCTACTG GAGAATATAC TAAGGGTACT GTTGACATTG
            601 CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG GGTGGAAGAG
            661 ATGAAGGTTA CGATTGGTTG ATTATGACAC CCGGTGTGGG TTTAGATGAC AAGGGAGACG
            721 CATTGGGTCA ACAGTATAGA ACCGTGGATG ATGTGGTCTC TACAGGNTCT GACATTATTA
            781 TTGTTGGAAG ANGACTATTT GCNAAGGGAA NGGANGCTAA NGTAGANGGT GAACGTTACA
            841 GAAAAGCANG CTGGGAAGCA TATTTGAGAA GANGCGGCCA GCAAANCTNA AAAANCNGTA
            901 TTATAAGNNA NTGNNNGTAN NCNNANNNCN NNANNTNNNN CTTCNNNTNN NTTNNNNNNN
          961 NTNNNNNNCN NNNNNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Ifa38 5')
          Sequence file: RDB16043_A8I3m.seq check
          >16043_16043_B0JcF_TDH3pro_F_H01_22_ABI24.ab1
              1 NNNNNNNNNN NNTGNNTGNN ATTCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT
             61 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC GGATGCGGCC
            121 GCATGACTTT TATGCAACAG CTTCAAGAGG CTGGGGAAAG ATTTAGGTGT ATCAATGGTC
            181 TTTTATGGGT TGTTTTTGGG CTTGGTGTCT TAAAATGTAC AACGTTATCA CTTAGATTTT
            241 TAGCTCTTAT TTTTGATCTT TTTTTACTAC CAGCAGTCAA TTTCGACAAG TATGGTGCTA
            301 AAACTGGTAA ATACTGTGCT ATCACTGGTG CAAGTGACGG TATTGGTAAA GAATTTGCTA
            361 GACAAATGGC GAAACGTGGC TTCAACTTGG TATTGATCTC GAGAACGCAA TCTAAATTGG
            421 AGGCTTTACA AAAAGAACTA GAAGATCAAC ATCATGTCGT TGTAAAGATT CTAGCCATTG
            481 ACATTGCGGA GGATAAAGAA TCCAACTATG AATCCATTAA GGAATTGTGT GCGCAGTTAC
            541 CAATCACCGT TTTGGTCAAT AATGTTGGTC AATCACACTC CATTCCTGTT CCATTTTTGG
            601 AAACAGAAGA GAANGANCTT ANAAATATTA TCACTATCNA TAACACTGCT ACATTATTAA
            661 TTACACNANT CATTGCACCN AAGANTGNGN AANCNGTGNA NNNTGAAAAN AANNANNTCG
          721 GNNNNNNNNN N
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP1-10)
          Sequence file: RDB16043_A8I3q.seq check
          >D05803_A7_A8I3_1_CYC1ter_R_G01_19_ABI24.ab1
              1 NNNNNNNNNN NGNNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGNNATAACT
             61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
            121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
            181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
            241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
            301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
            361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
            421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
            481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
            541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
            601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
            661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGNGA
            721 CAACTCCAGT GAAAAGTTCT TCTCCNTTGC TCATACTAGT GCCACCCATT GATNCGCCAC
            781 CAGANCCTCN NGNNNCTTCC TTTTTAACCN GTCTTGCGGC TTTTTTTNAN NNNTCTAATT
          841 CTGANAGANT TATNGANNNN ANNNNTTNAN NNNNTTNACN NNNNNNNNN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB16043_A8I3r.seq check
          >D05803_A7_A8I3_1_Amp_R_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNNNANNGNN NCANTTATNN GGGTNATTGT CTCATGNGCG GANACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
            181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
            241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
            301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
            361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
            421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
            481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
            541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
            601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
            661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
            721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA
            781 AGCGGATGCC GGGANCAGAN AANCCCGTCA NGGCGCGTCA NCGGGNGTTG GCGGGNGTCG
            841 GGGGCTGGCT TNACTATGCG GCATCAGANC AGANNGTACT GANANTGCAC CATACCNCCN
            901 TTTTCNNNTC ATCATTTTTT TTTTTANNCN TTTTTTTNNG ANTTCNNNTN NCNTNNNANN
          961 TTNTTTNNNN NTNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl