Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.
Catalog number | RDB16043 |
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Resource name | Ifa38-GFP1-10 |
Alternative name | pRS316-GPDp-Ifa38-GFP1-10 (pSFL16) |
Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS316 (plasmid) |
Selectable markers | Amp^r (E. coli), URA3 |
Gene/insert name | S. cerevisiae IFA38 cDNA |
Depositor|Developer | Tamura, Yasushi | |
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Other clones in our bank | S. cerevisiae IFA38 (KEGG orthology K10251) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Schedule A (GFP-A) [Link] |
Remarks | Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16043 | Ifa38-GFP1-10 | DNA solution |
Electronic file
Original reference
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Dnaconda's recommendation EXR0090j (Japanese text) |
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Featured content | Dnaconda's recommendation EXR0090e (English text) |
Featured content | Visualization of Organelles (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16043_A8I3p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: HB-NOS_R Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB16043_A8I3i.seq ![]() |
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>16043_16043_B0JcF_HB-NOS_R_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG GTAACGCCNG GGTTTTCCNN GTCACGACGT 61 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCAGT 121 TTATCATTAT CAATACTCGC CATTTCAAAG AATACGTAAA TAATTAATAG TAGTGATTTT 181 CCTAACTTTA TTTAGTCAAA AAATTAGCCT TTTAATTCTG CTGTAACCCG TACATGCCCA 241 AAATAGGGGG CGGGTTACAC AGAATATATA ACATCGTAGG TGTCTGGGTG AACAGTTTAT 301 TCCTGGCATC CACTAAATAT AATGGAGCCC GCTTTTTAAG CTGGCATCCA GAAAAAAAAA 361 GAATCCCAGC ACCAAAATAT TGTTTTCTTC ACCAACCATC AGTTCATAGG TCCATTCTCT 421 TAGCGCAACT ACAGAGAACA GGGGCACAAA CAGGCAAAAA ACGGGCACAA CCTCAATGGA 481 GTGATGCAAC CTGCCTGGAG TAAATGATGA CACAAGGCAA TTGANCCACG CATGTATCTA 541 TCTCATTTTC TTACNNNTTC TATTACCTTC NGCTCTCTCT GATTTGGAAA AAGCTGAAAA 601 AAAANGNTGN NNCANTTCCC TGAAANTATT CCCCTACTNN ACTNNNANNA NNNNANNANN 661 NNANGNANNN NNNNNNN // |
Primer: SV40pA3'Element Reverse Region: T3 pro, insert 3' (CYC1 ter) Sequence file: RDB16043_A8I3j.seq ![]() |
>16043_16043_B0JcF_SV40pA3'elementReverse_A02_02_ABI24.ab1 1 NNNNNNNNGN NNNNNNNNNA NGNNNNNNCN NGCAACGCGG CCTTTTTACG GTTCCTGGCC 61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA 481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT 541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA 601 CGTTCTTAAT ACTAACATAA CTATNAAAAA AATAAATANG GANCTANACT TCNNNNTGTC 661 NNANTCCNTC CNTTTTNNNN NANNNCNNNN GTNNNNGNNG NNNNGNANNG NNNNCNNNNN 721 NNNNN // |
Primer: paGFP-M_R1 Region: insert mid (GFP1-10 5', Ifa38 3') Sequence file: RDB16043_A8I3k.seq ![]() |
>16043_16043_B0JcF_paGFP-M_R1_G01_19_ABI24.ab1 1 NNNNNNNNNN NAANNNNTGC CTTTTNNTGT GNTCCGGANA ACGGGAAAAG CATTGAACAC 61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA 121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA 181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA 241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC 301 CTTCCTTTTT AACCTGTCTT GCGGCTTTTT TTAAGGCTCT AATTCTGATA GATTTATGGA 361 AGGAATAATT AATGGAGTTA ACAATCTTAG AGTAAACGCC AAAGGTCTCT GTAATTACAA 421 ATTGATACAC TGCGTGCGCC CAGTAAGGGG TCATAGTAGC GTATCTTTCT TGAGAGCCGC 481 AGCGTCTGCC AACGCTTCTT AAAGTGGATT TTACAAACTG TTGTGGATTT GGGATCATTA 541 AAGATGATCT TCTGATTTTA GACATCGAGC TAGTGACCAA ATATGAAATG ATTAATTCAA 601 CATCGATAGC ATCTTTAGAT AATTCCCCAG CCAAAGAGTT AGACCAACCT TGTAAGANNG 661 ANTTCNAACC NCTGTATGTA GNCNNAAAGT GGGGNGGGAA TCNNANCACC AAATGANCCC 721 NNNGNTNAAN NNCAAANNNN NANNNNCNNN NN // |
Primer: URA3pro_F Region: URA3pro, URA3 Sequence file: RDB16043_A8I3l.seq ![]() |
>D05803_A7_A8I3_1_URA3pro_F_C03_09_ABI24.ab1 1 CAAGGCTAGT GTTGAGAAAC ATGAATTGCC CAGTATTCTT AACCCAACTG CACAGAACAA 61 AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA 121 CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT 181 TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG 241 GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG 301 GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA 361 GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA 421 TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG 481 GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG ATGTTAGCAG 541 AATTGTCATG CAAGGGCTCC CTATCTACTG GAGAATATAC TAAGGGTACT GTTGACATTG 601 CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG GGTGGAAGAG 661 ATGAAGGTTA CGATTGGTTG ATTATGACAC CCGGTGTGGG TTTAGATGAC AAGGGAGACG 721 CATTGGGTCA ACAGTATAGA ACCGTGGATG ATGTGGTCTC TACAGGATCT GACATTATTA 781 TTGTTGGAAG AGGACTATTT GCAAAGGGAA GGGATGCTAA GGTAGAGGGT GAACGTTACA 841 GAAAAGCAGG CTGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA 901 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG 961 TTTATTACCC TATGCGGTGT GAAATACCGC ACAGATGCGT AAAGGAGAAA AATACCGCAT 1021 CAGGAAATTT GTAAGCGTTA ATATTTTGAT AAAATTCGCG TCAATTTTTG GTAAATCAGC 1081 TCATTTTTTT AACCA // |
Primer: TDH3pro_F Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB16043_A8I3m.seq ![]() |
>16043_16043_B0JcF_TDH3pro_F_H01_22_ABI24.ab1 1 NNNNNNNNNN NNTGNNTGNN ATTCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT 61 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC GGATGCGGCC 121 GCATGACTTT TATGCAACAG CTTCAAGAGG CTGGGGAAAG ATTTAGGTGT ATCAATGGTC 181 TTTTATGGGT TGTTTTTGGG CTTGGTGTCT TAAAATGTAC AACGTTATCA CTTAGATTTT 241 TAGCTCTTAT TTTTGATCTT TTTTTACTAC CAGCAGTCAA TTTCGACAAG TATGGTGCTA 301 AAACTGGTAA ATACTGTGCT ATCACTGGTG CAAGTGACGG TATTGGTAAA GAATTTGCTA 361 GACAAATGGC GAAACGTGGC TTCAACTTGG TATTGATCTC GAGAACGCAA TCTAAATTGG 421 AGGCTTTACA AAAAGAACTA GAAGATCAAC ATCATGTCGT TGTAAAGATT CTAGCCATTG 481 ACATTGCGGA GGATAAAGAA TCCAACTATG AATCCATTAA GGAATTGTGT GCGCAGTTAC 541 CAATCACCGT TTTGGTCAAT AATGTTGGTC AATCACACTC CATTCCTGTT CCATTTTTGG 601 AAACAGAAGA GAANGANCTT ANAAATATTA TCACTATCNA TAACACTGCT ACATTATTAA 661 TTACACNANT CATTGCACCN AAGANTGNGN AANCNGTGNA NNNTGAAAAN AANNANNTCG 721 GNNNNNNNNN N // |
Primer: CYC1ter_R Region: insert mid (CYC1 ter, GFP1-10, Ifa38 3') Sequence file: RDB16043_A8I3n.seq ![]() |
>D05803_A7_A8I3_1_CYC1ter_R_G01_19_ABI24.ab1 1 ATTTCGTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT 61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT 121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG 181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT 241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA 301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT 361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA 421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT 481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG 541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT 601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT 661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA 721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC 781 CAGACCCTCC GGATCCTTCC TTTTTAACCT GTCTTGCGGC TTTTTTTAAA GGCTCTAATT 841 CTGATAGATT TATGGAAGGA AATAATTAAT GGAGTTAACA ATCTTAGAGT AAACGCCCAA 901 AGGTCTCTGT AATTACAAAT TTGATACACT GCGTGCGCCC AGTAAAGGGT CATAGTAGCG 961 TATCTTTTCT TGAGAGCCGC AGCCGTCTGC GACGCTTCTA AGTGATTTTA CAACTGTTGT 1021 GAATTGGAAT CATGAGATGA TCTTCTGATT TTTAGAACAT CGAAGCT // |
Primer: Amp_R Region: AmpR pro, CEN_ARS Sequence file: RDB16043_A8I3o.seq ![]() |
>D05803_A7_A8I3_1_Amp_R_D03_12_ABI24.ab1 1 TCCGTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA 181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT 241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT 301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG 361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC 421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT 481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT 541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT 601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT 661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG 721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA 781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG 841 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATACCACCT 901 TTTTCAATTC ATCATTTTTT TTTTTATTCT TTTTTTTTTG ATTTCGGTTT CCCTTGAAAT 961 TTTTTTTTGA TTTCGGTAAT CTCCCGAACA GAACGAAGAC GAACGGATGG AGCACAGAAC 1021 TTTAGAATTT GGTATATATA CGCATATTGT AGTGGATGGA GGAAACATGC AATGCCAGTA 1081 TTCCTAACCC AACCTGCACA GGAAACA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. |
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2020.11.09
GNP_filter3_RDBDEP_html_201104.pl