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Ifa38-GFP1-10

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Catalog number RDB16043
Resource name Ifa38-GFP1-10
Alternative name pRS316-GPDp-Ifa38-GFP1-10 (pSFL16)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae IFA38 cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae IFA38 (KEGG orthology K10251) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16043 Ifa38-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16043_A8I3p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB16043_A8I3i.seq check
>16043_16043_B0JcF_HB-NOS_R_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG GTAACGCCNG GGTTTTCCNN GTCACGACGT
   61 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCAGT
  121 TTATCATTAT CAATACTCGC CATTTCAAAG AATACGTAAA TAATTAATAG TAGTGATTTT
  181 CCTAACTTTA TTTAGTCAAA AAATTAGCCT TTTAATTCTG CTGTAACCCG TACATGCCCA
  241 AAATAGGGGG CGGGTTACAC AGAATATATA ACATCGTAGG TGTCTGGGTG AACAGTTTAT
  301 TCCTGGCATC CACTAAATAT AATGGAGCCC GCTTTTTAAG CTGGCATCCA GAAAAAAAAA
  361 GAATCCCAGC ACCAAAATAT TGTTTTCTTC ACCAACCATC AGTTCATAGG TCCATTCTCT
  421 TAGCGCAACT ACAGAGAACA GGGGCACAAA CAGGCAAAAA ACGGGCACAA CCTCAATGGA
  481 GTGATGCAAC CTGCCTGGAG TAAATGATGA CACAAGGCAA TTGANCCACG CATGTATCTA
  541 TCTCATTTTC TTACNNNTTC TATTACCTTC NGCTCTCTCT GATTTGGAAA AAGCTGAAAA
  601 AAAANGNTGN NNCANTTCCC TGAAANTATT CCCCTACTNN ACTNNNANNA NNNNANNANN
661 NNANGNANNN NNNNNNN
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter)
Sequence file: RDB16043_A8I3j.seq check
>16043_16043_B0JcF_SV40pA3'elementReverse_A02_02_ABI24.ab1
    1 NNNNNNNNGN NNNNNNNNNA NGNNNNNNCN NGCAACGCGG CCTTTTTACG GTTCCTGGCC
   61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
  121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
  181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
  241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
  301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG
  361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC
  421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA
  481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT
  541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA
  601 CGTTCTTAAT ACTAACATAA CTATNAAAAA AATAAATANG GANCTANACT TCNNNNTGTC
  661 NNANTCCNTC CNTTTTNNNN NANNNCNNNN GTNNNNGNNG NNNNGNANNG NNNNCNNNNN
721 NNNNN
//
Primer: paGFP-M_R1
Region: insert mid (GFP1-10 5', Ifa38 3')
Sequence file: RDB16043_A8I3k.seq check
>16043_16043_B0JcF_paGFP-M_R1_G01_19_ABI24.ab1
    1 NNNNNNNNNN NAANNNNTGC CTTTTNNTGT GNTCCGGANA ACGGGAAAAG CATTGAACAC
   61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA
  121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA
  181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA
  241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC
  301 CTTCCTTTTT AACCTGTCTT GCGGCTTTTT TTAAGGCTCT AATTCTGATA GATTTATGGA
  361 AGGAATAATT AATGGAGTTA ACAATCTTAG AGTAAACGCC AAAGGTCTCT GTAATTACAA
  421 ATTGATACAC TGCGTGCGCC CAGTAAGGGG TCATAGTAGC GTATCTTTCT TGAGAGCCGC
  481 AGCGTCTGCC AACGCTTCTT AAAGTGGATT TTACAAACTG TTGTGGATTT GGGATCATTA
  541 AAGATGATCT TCTGATTTTA GACATCGAGC TAGTGACCAA ATATGAAATG ATTAATTCAA
  601 CATCGATAGC ATCTTTAGAT AATTCCCCAG CCAAAGAGTT AGACCAACCT TGTAAGANNG
  661 ANTTCNAACC NCTGTATGTA GNCNNAAAGT GGGGNGGGAA TCNNANCACC AAATGANCCC
721 NNNGNTNAAN NNCAAANNNN NANNNNCNNN NN
//
Primer: URA3pro_F
Region: URA3pro, URA3
Sequence file: RDB16043_A8I3l.seq check
>D05803_A7_A8I3_1_URA3pro_F_C03_09_ABI24.ab1
    1 CAAGGCTAGT GTTGAGAAAC ATGAATTGCC CAGTATTCTT AACCCAACTG CACAGAACAA
   61 AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA
  121 CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT
  181 TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG
  241 GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG
  301 GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA
  361 GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA
  421 TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG
  481 GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG ATGTTAGCAG
  541 AATTGTCATG CAAGGGCTCC CTATCTACTG GAGAATATAC TAAGGGTACT GTTGACATTG
  601 CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG GGTGGAAGAG
  661 ATGAAGGTTA CGATTGGTTG ATTATGACAC CCGGTGTGGG TTTAGATGAC AAGGGAGACG
  721 CATTGGGTCA ACAGTATAGA ACCGTGGATG ATGTGGTCTC TACAGGATCT GACATTATTA
  781 TTGTTGGAAG AGGACTATTT GCAAAGGGAA GGGATGCTAA GGTAGAGGGT GAACGTTACA
  841 GAAAAGCAGG CTGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA
  901 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG
  961 TTTATTACCC TATGCGGTGT GAAATACCGC ACAGATGCGT AAAGGAGAAA AATACCGCAT
 1021 CAGGAAATTT GTAAGCGTTA ATATTTTGAT AAAATTCGCG TCAATTTTTG GTAAATCAGC
1081 TCATTTTTTT AACCA
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, Ifa38 5')
Sequence file: RDB16043_A8I3m.seq check
>16043_16043_B0JcF_TDH3pro_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNTGNNTGNN ATTCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT
   61 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC GGATGCGGCC
  121 GCATGACTTT TATGCAACAG CTTCAAGAGG CTGGGGAAAG ATTTAGGTGT ATCAATGGTC
  181 TTTTATGGGT TGTTTTTGGG CTTGGTGTCT TAAAATGTAC AACGTTATCA CTTAGATTTT
  241 TAGCTCTTAT TTTTGATCTT TTTTTACTAC CAGCAGTCAA TTTCGACAAG TATGGTGCTA
  301 AAACTGGTAA ATACTGTGCT ATCACTGGTG CAAGTGACGG TATTGGTAAA GAATTTGCTA
  361 GACAAATGGC GAAACGTGGC TTCAACTTGG TATTGATCTC GAGAACGCAA TCTAAATTGG
  421 AGGCTTTACA AAAAGAACTA GAAGATCAAC ATCATGTCGT TGTAAAGATT CTAGCCATTG
  481 ACATTGCGGA GGATAAAGAA TCCAACTATG AATCCATTAA GGAATTGTGT GCGCAGTTAC
  541 CAATCACCGT TTTGGTCAAT AATGTTGGTC AATCACACTC CATTCCTGTT CCATTTTTGG
  601 AAACAGAAGA GAANGANCTT ANAAATATTA TCACTATCNA TAACACTGCT ACATTATTAA
  661 TTACACNANT CATTGCACCN AAGANTGNGN AANCNGTGNA NNNTGAAAAN AANNANNTCG
721 GNNNNNNNNN N
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, GFP1-10, Ifa38 3')
Sequence file: RDB16043_A8I3n.seq check
>D05803_A7_A8I3_1_CYC1ter_R_G01_19_ABI24.ab1
    1 ATTTCGTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
  121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
  181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
  241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
  301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
  361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
  421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
  481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
  541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
  601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
  661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
  721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
  781 CAGACCCTCC GGATCCTTCC TTTTTAACCT GTCTTGCGGC TTTTTTTAAA GGCTCTAATT
  841 CTGATAGATT TATGGAAGGA AATAATTAAT GGAGTTAACA ATCTTAGAGT AAACGCCCAA
  901 AGGTCTCTGT AATTACAAAT TTGATACACT GCGTGCGCCC AGTAAAGGGT CATAGTAGCG
  961 TATCTTTTCT TGAGAGCCGC AGCCGTCTGC GACGCTTCTA AGTGATTTTA CAACTGTTGT
1021 GAATTGGAAT CATGAGATGA TCTTCTGATT TTTAGAACAT CGAAGCT
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB16043_A8I3o.seq check
>D05803_A7_A8I3_1_Amp_R_D03_12_ABI24.ab1
    1 TCCGTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
  181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
  241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
  301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
  361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
  421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
  481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
  541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
  601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
  661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
  721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA
  781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG
  841 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATACCACCT
  901 TTTTCAATTC ATCATTTTTT TTTTTATTCT TTTTTTTTTG ATTTCGGTTT CCCTTGAAAT
  961 TTTTTTTTGA TTTCGGTAAT CTCCCGAACA GAACGAAGAC GAACGGATGG AGCACAGAAC
 1021 TTTAGAATTT GGTATATATA CGCATATTGT AGTGGATGGA GGAAACATGC AATGCCAGTA
1081 TTCCTAACCC AACCTGCACA GGAAACA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.11.09

GNP_filter3_RDBDEP_html_201104.pl