Please review the QC test results indicated by icon below as well as clone information before placing your order.

Ifa38-GFP1-10

Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16043_A8I3p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB16043_A8I3i.seq
>16043_16043_B0JcF_HB-NOS_R_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG GTAACGCCNG GGTTTTCCNN GTCACGACGT    61 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCAGT   121 TTATCATTAT CAATACTCGC CATTTCAAAG AATACGTAAA TAATTAATAG TAGTGATTTT   181 CCTAACTTTA TTTAGTCAAA AAATTAGCCT TTTAATTCTG CTGTAACCCG TACATGCCCA   241 AAATAGGGGG CGGGTTACAC AGAATATATA ACATCGTAGG TGTCTGGGTG AACAGTTTAT   301 TCCTGGCATC CACTAAATAT AATGGAGCCC GCTTTTTAAG CTGGCATCCA GAAAAAAAAA   361 GAATCCCAGC ACCAAAATAT TGTTTTCTTC ACCAACCATC AGTTCATAGG TCCATTCTCT   421 TAGCGCAACT ACAGAGAACA GGGGCACAAA CAGGCAAAAA ACGGGCACAA CCTCAATGGA   481 GTGATGCAAC CTGCCTGGAG TAAATGATGA CACAAGGCAA TTGANCCACG CATGTATCTA   541 TCTCATTTTC TTACNNNTTC TATTACCTTC NGCTCTCTCT GATTTGGAAA AAGCTGAAAA   601 AAAANGNTGN NNCANTTCCC TGAAANTATT CCCCTACTNN ACTNNNANNA NNNNANNANN 661 NNANGNANNN NNNNNNN //
Primer: SV40pA3'Element Reverse Region: T3 pro, insert 3' (CYC1 ter) Sequence file: RDB16043_A8I3j.seq
>16043_16043_B0JcF_SV40pA3'elementReverse_A02_02_ABI24.ab1     1 NNNNNNNNGN NNNNNNNNNA NGNNNNNNCN NGCAACGCGG CCTTTTTACG GTTCCTGGCC    61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC   121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC   181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT   241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG   301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG   361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC   421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA   481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT   541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA   601 CGTTCTTAAT ACTAACATAA CTATNAAAAA AATAAATANG GANCTANACT TCNNNNTGTC   661 NNANTCCNTC CNTTTTNNNN NANNNCNNNN GTNNNNGNNG NNNNGNANNG NNNNCNNNNN 721 NNNNN //
Primer: paGFP-M_R1 Region: insert mid (GFP1-10 5', Ifa38 3') Sequence file: RDB16043_A8I3k.seq
>16043_16043_B0JcF_paGFP-M_R1_G01_19_ABI24.ab1     1 NNNNNNNNNN NAANNNNTGC CTTTTNNTGT GNTCCGGANA ACGGGAAAAG CATTGAACAC    61 CATAGGTCAG AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA   121 TAAATTTAAG TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA   181 ATTTGTGCCC ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA   241 GTTCTTCTCC TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC   301 CTTCCTTTTT AACCTGTCTT GCGGCTTTTT TTAAGGCTCT AATTCTGATA GATTTATGGA   361 AGGAATAATT AATGGAGTTA ACAATCTTAG AGTAAACGCC AAAGGTCTCT GTAATTACAA   421 ATTGATACAC TGCGTGCGCC CAGTAAGGGG TCATAGTAGC GTATCTTTCT TGAGAGCCGC   481 AGCGTCTGCC AACGCTTCTT AAAGTGGATT TTACAAACTG TTGTGGATTT GGGATCATTA   541 AAGATGATCT TCTGATTTTA GACATCGAGC TAGTGACCAA ATATGAAATG ATTAATTCAA   601 CATCGATAGC ATCTTTAGAT AATTCCCCAG CCAAAGAGTT AGACCAACCT TGTAAGANNG   661 ANTTCNAACC NCTGTATGTA GNCNNAAAGT GGGGNGGGAA TCNNANCACC AAATGANCCC 721 NNNGNTNAAN NNCAAANNNN NANNNNCNNN NN //
Primer: URA3pro_F Region: URA3pro, URA3 Sequence file: RDB16043_A8I3l.seq
>D05803_A7_A8I3_1_URA3pro_F_C03_09_ABI24.ab1     1 CAAGGCTAGT GTTGAGAAAC ATGAATTGCC CAGTATTCTT AACCCAACTG CACAGAACAA    61 AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA   121 CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT   181 TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG   241 GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG   301 GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA   361 GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA   421 TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG   481 GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG ATGTTAGCAG   541 AATTGTCATG CAAGGGCTCC CTATCTACTG GAGAATATAC TAAGGGTACT GTTGACATTG   601 CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG GGTGGAAGAG   661 ATGAAGGTTA CGATTGGTTG ATTATGACAC CCGGTGTGGG TTTAGATGAC AAGGGAGACG   721 CATTGGGTCA ACAGTATAGA ACCGTGGATG ATGTGGTCTC TACAGGATCT GACATTATTA   781 TTGTTGGAAG AGGACTATTT GCAAAGGGAA GGGATGCTAA GGTAGAGGGT GAACGTTACA   841 GAAAAGCAGG CTGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA   901 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG   961 TTTATTACCC TATGCGGTGT GAAATACCGC ACAGATGCGT AAAGGAGAAA AATACCGCAT  1021 CAGGAAATTT GTAAGCGTTA ATATTTTGAT AAAATTCGCG TCAATTTTTG GTAAATCAGC 1081 TCATTTTTTT AACCA //
Primer: TDH3pro_F Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB16043_A8I3m.seq
>16043_16043_B0JcF_TDH3pro_F_H01_22_ABI24.ab1     1 NNNNNNNNNN NNTGNNTGNN ATTCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT    61 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC GGATGCGGCC   121 GCATGACTTT TATGCAACAG CTTCAAGAGG CTGGGGAAAG ATTTAGGTGT ATCAATGGTC   181 TTTTATGGGT TGTTTTTGGG CTTGGTGTCT TAAAATGTAC AACGTTATCA CTTAGATTTT   241 TAGCTCTTAT TTTTGATCTT TTTTTACTAC CAGCAGTCAA TTTCGACAAG TATGGTGCTA   301 AAACTGGTAA ATACTGTGCT ATCACTGGTG CAAGTGACGG TATTGGTAAA GAATTTGCTA   361 GACAAATGGC GAAACGTGGC TTCAACTTGG TATTGATCTC GAGAACGCAA TCTAAATTGG   421 AGGCTTTACA AAAAGAACTA GAAGATCAAC ATCATGTCGT TGTAAAGATT CTAGCCATTG   481 ACATTGCGGA GGATAAAGAA TCCAACTATG AATCCATTAA GGAATTGTGT GCGCAGTTAC   541 CAATCACCGT TTTGGTCAAT AATGTTGGTC AATCACACTC CATTCCTGTT CCATTTTTGG   601 AAACAGAAGA GAANGANCTT ANAAATATTA TCACTATCNA TAACACTGCT ACATTATTAA   661 TTACACNANT CATTGCACCN AAGANTGNGN AANCNGTGNA NNNTGAAAAN AANNANNTCG 721 GNNNNNNNNN N //
Primer: CYC1ter_R Region: insert mid (CYC1 ter, GFP1-10, Ifa38 3') Sequence file: RDB16043_A8I3n.seq
>D05803_A7_A8I3_1_CYC1ter_R_G01_19_ABI24.ab1     1 ATTTCGTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT    61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT   121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG   181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT   241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA   301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT   361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA   421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT   481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG   541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT   601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT   661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA   721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC   781 CAGACCCTCC GGATCCTTCC TTTTTAACCT GTCTTGCGGC TTTTTTTAAA GGCTCTAATT   841 CTGATAGATT TATGGAAGGA AATAATTAAT GGAGTTAACA ATCTTAGAGT AAACGCCCAA   901 AGGTCTCTGT AATTACAAAT TTGATACACT GCGTGCGCCC AGTAAAGGGT CATAGTAGCG   961 TATCTTTTCT TGAGAGCCGC AGCCGTCTGC GACGCTTCTA AGTGATTTTA CAACTGTTGT 1021 GAATTGGAAT CATGAGATGA TCTTCTGATT TTTAGAACAT CGAAGCT //
Primer: Amp_R Region: AmpR pro, CEN_ARS Sequence file: RDB16043_A8I3o.seq
>D05803_A7_A8I3_1_Amp_R_D03_12_ABI24.ab1     1 TCCGTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA   181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT   241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT   301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG   361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC   421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT   481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT   541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT   601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT   661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG   721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA   781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG   841 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATACCACCT   901 TTTTCAATTC ATCATTTTTT TTTTTATTCT TTTTTTTTTG ATTTCGGTTT CCCTTGAAAT   961 TTTTTTTTGA TTTCGGTAAT CTCCCGAACA GAACGAAGAC GAACGGATGG AGCACAGAAC  1021 TTTAGAATTT GGTATATATA CGCATATTGT AGTGGATGGA GGAAACATGC AATGCCAGTA 1081 TTCCTAACCC AACCTGCACA GGAAACA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.11.09

GNP_filter3_RDBDEP_html_201104.pl