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Tom70N-FLAG-GFP1-10

Expression vector of human translocase of outer mitochondrial membrane 70 (Tom70), N-terminal segments 1-70.

Catalog number RDB16042
Resource name Tom70N-FLAG-GFP1-10
Alternative name pcDNA3.1-Tom70N-3xFLAG-GFP1-10 (pMM82)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 70 (Tom70), N-terminal segments 1-70 fused with 3xFLAG-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human TOMM70 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank human TOMM70 (NCBI Gene 9868) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
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RDB16042 Tom70N-FLAG-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16042_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB16042_A8I3a.seq check
>D05803_A6_A8I3_1_CMV-Forward_D08_11_ABI24.ab1
    1 CTTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CGAGCTCGGA
  181 TCCGCCACCA TGGCCGCGTC CAAACCCGTG GAAGCAGCCG TCGTTGCAGC CGCTGTGCCG
  241 AGCTCTGGGT CAGGCGTGGG TGGAGGAGGA ACCGCTGGTC CCGGAACAGG CGGCCTTCCT
  301 CGGTGGCAGT TGGCCCTGGC TGTAGGGGCA CCACTGCTCC TGGGAGCTGG GGCCATCTAT
  361 CTGTGGAGCC GCCAACAGCG TCGAAGGGAG GCCAGAGGGG ACTATAAGGA CCATGATGGG
  421 GATTACAAGG ATCACGACAT TGACTACAAG GATGACGACG ATAAAGGCGG TAGTGGCGGC
  481 GCGGCCGCCG GTGGCACTAG TATGAGCAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA
  541 ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG AGGAGAGGGT
  601 GAAGGTGATG CTACAATCGG AAAACTCACA CTTAAATTTA TTTGCACTAC TGGAAAACTA
  661 CCTGTTCCAT GGCCAACACT TGTCACTACT CTGACCTATG GTGTTCAATG CTTTTCCCGT
  721 TATCCGGATC ACATGAAAAG GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA
  781 CAGGAACGCA CTATATCTTT CAAAGATGAC GGGAAATACA AGACGCGTGC TGTAGTCAAG
  841 TTTGAAAGGT GATACCCTTG TTAATCGTAT CGAGTTAAAG GGTACTGATT TTTAAGAAGA
  901 TGGAAACATT CTCGGACACA AACTCGAGTA CACTTTTAAC TCACACAATG TATACATCAC
  961 GGGCAGACAA CAAAGATGGC ATCAAAGCTA ACCTTCACAG GTCGCCAACA CGGTTGGAGG
1021 ATTGCATCCC GGTTCACTAG CAGATCGATA TCCACCGAAT ACTCCGAATT GC
//
Primer: BGH_rev2
Sequence file: RDB16042_A8I3b.seq check
>D05803_A6_A8I3_1_BGH_rev2_E08_14_ABI24.ab1
    1 TATAGTTAAG ATGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGATTA CTTTTCGTTG GGATCTTTCG
  121 AAAGGACAGT TTGTGTCGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG CCATCGCCAA
  181 TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGGA ACCATCTTCA ACGTTGTGGC
  241 GAACTGTGAA GTTAGCTTTG ATTCCATTCT TTTGTTTGTC TGCCGTGATG TATACATTGT
  301 GTGAGTTAAA GTTGTACTCG AGTTTGTGTC CGAGAATGTT TCCATCTTCT TTAAAATCAG
  361 TACCCTTTAA CTCGATACGA TTAACAAGGG TATCACCTTC AAACTTGACT ACAGCACGCG
  421 TCTTGTATTT CCCGTCATCT TTGAAAGATA TAGTGCGTTC CTGTACATAA CCTTCGGGCA
  481 TGGCACTCTT GAAAAAGTCA TGCCTTTTCA TGTGATCCGG ATAACGGGAA AAGCATTGAA
  541 CACCATAGGT CAGAGTAGTG ACAAGTGTTG GCCATGGAAC AGGTAGTTTT CCAGTAGTGC
  601 AAATAAATTT AAGTGTGAGT TTTCCGATTG TAGCATCACC TTCACCCTCT CCTCTGACAG
  661 AAAATTTGTG CCCATTAACA TCACCATCTA ATTCAACAAG AATTGGGACA ACTCCAGTGA
  721 AAAGTTCTTC TCCTTTGCTC ATACTAGTGC CACCGGCGGC CGCGCCGCCA CTACCGCCTT
  781 TATCGTCGTC ATCCTTGTAG TCAATGTCGT GATCCTTGTA ATCCCCATCA TGGTCCTTAT
  841 AGTCCCCTCT GGCCTCCCTT CGACGCTGTT GGCGGCTCCA CAGATAGATG GCCCCAGCTC
  901 CCAGGAGCAG TGGTGCCCCT ACAGCCAGGG CCCAACTGCC ACCGAGGAAG GCCGCCCTGT
  961 TCCCGGGACC CAGCGGGTTC CTCCCTCCAC CCACGCCCTG ACCCAGAGCT CGGCACAGCG
 1021 CTGCAACGGA CCGGCCTGCC TTTCCACGGG TTGACGGCGG TCATGCCTGG CCGAATTCGA
1081 GTCTCGGTAC TAAGCTTAAG G
//
Primer: SV40pro_F
Sequence file: RDB16042_A8I3c.seq check
>D05803_A6_A8I3_1_SV40pro_F_F08_17_ABI24.ab1
    1 CCCTGTCCGG GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CCACCGCCCG CCTTTCCTAT GAAGGGTTGG
 1021 GCCTTCGGAT TCGTTTTCCC GGGGACGCTG GCTGGTATGC ATCCTTCCCA GCCGACGGGG
1081 GTATTCAC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl