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Tom70N-FLAG-GFP1-10 (#RDB16042)

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: 3xFLAG-GFP1-10; location: mitochondria.

Alternative name pMM82, pcDNA3.1-Tom70N-3xFLAG-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 70 (Tom70), N-terminal segments 1-70 fused with 3xFLAG-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human TOMM70 cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB16042hts01.seq checkAssembled from experimentally sequenced data.
Publication Kakimoto, Y., Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]
Test sheet RDB16042_A9Bdp1-5.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16042 Tom70N-FLAG-GFP1-10 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The Tom70N-FLAG-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16042).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]