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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Tom70N-FLAG-GFP1-10

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: 3xFLAG-GFP1-10; location: mitochondria.

Catalog number RDB16042
Resource name Tom70N-FLAG-GFP1-10
Alternative name pMM82, pcDNA3.1-Tom70N-3xFLAG-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 70 (Tom70), N-terminal segments 1-70 fused with 3xFLAG-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human TOMM70 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank
External Database
human TOMM70
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
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          RDB16042 Tom70N-FLAG-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Tom70N-FLAG-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16042).

          Reference section:

          Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16042_A8I3p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'(Tom70(1-70), 3XFLAG)
          Sequence file: RDB16042_A8I3e.seq check
          >D05803_A6_A8I3_1_CMV-Forward_D08_11_ABI24.ab1
              1 NNNNNNNNNN NNCNTTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGNNG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CGAGCTCGGA
            181 TCCGCCACCA TGGCCGCGTC CAAACCCGTG GAAGCAGCCG TCGTTGCAGC CGCTGTGCCG
            241 AGCTCTGGGT CAGGCGTGGG TGGAGGAGGA ACCGCTGGTC CCGGAACAGG CGGCCTTCCT
            301 CGGTGGCAGT TGGCCCTGGC TGTAGGGGCA CCACTGCTCC TGGGAGCTGG GGCCATCTAT
            361 CTGTGGAGCC GCCAACAGCG TCGAAGGGAG GCCAGAGGGG ACTATAAGGA CCATGATGGG
            421 GATTACAAGG ATCACGACAT TGACTACAAG GATGACGACG ATNAAGGCGG TAGTGGCGGC
            481 GCGGCCGCCG GTGGCACTAG TATGAGCAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA
            541 ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG AGGAGAGGGT
            601 GAAGGTGATG CTACAATCGG AAAACTCACA CTTAAATTTA TTTGCACTAC TGGAAAACTA
            661 CCTGTTCCAT GGCCNACACT TGTCACTACT CTGACCTATG GTGTTCAATG CTTTTCCCGT
            721 TATCCGGATC ACATGAAAAG GCATGACTTT TTCNAGANTG CCATGCCNGA ANGTTATGTA
            781 CAGGAACGCA CTATATCTTT CAAAGATGAN NGGAAATACN NGANGNNNGC TGNANNCNNG
            841 TTNGNANNNN GANNNCNNNN NTNNNNGNAN NNNNTTAAAG GGNNNNNNNT TTNAANNNNN
          901 NGGNANNNNN NNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,insert 3'(GFP1-10 3')
          Sequence file: RDB16042_A8I3f.seq check
          >D05803_A6_A8I3_1_BGH_rev2_E08_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGGGG GNNGGGCNNN CAACAGATGG CTGGCAACTA GAAGGCACAG
             61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGATTA CTTTTCGTTG GGATCTTTCG
            121 AAAGGACAGT TTGTGTCGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG CCATCGCCAA
            181 TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGGA ACCATCTTCA ACGTTGTGGC
            241 GAACTGTGAA GTTAGCTTTG ATTCCATTCT TTTGTTTGTC TGCCGTGATG TATACATTGT
            301 GTGAGTTAAA GTTGTACTCG AGTTTGTGTC CGAGAATGTT TCCATCTTCT TTAAAATCAG
            361 TACCCTTTAA CTCGATACGA TTAACAAGGG TATCACCTTC AAACTTGACT ACAGCACGCG
            421 TCTTGTATTT CCCGTCATCT TTGAAAGATA TAGTGCGTTC CTGTACATAA CCTTCGGGCA
            481 TGGCACTCTT GAAAAAGTCA TGCCTTTTCA TGTGATCCGG ATAACGGGAA AAGCATTGAA
            541 CACCATAGGT CAGAGTAGTG ACAAGTGTTG GCCATGGAAC AGGTAGTTTT CCAGTAGTGC
            601 AAATAAATTT AAGTGTGAGT TTTCCGATTG TAGCATCACC TTCACCCTCT CCTCTGACAG
            661 AAAATTTGTG CCCATTAACA TCACCATCTA ATTCAACAAG AANTGGGACA ACTCCNGTGA
            721 AAAGTTCTTC TCCTTTGCTC ATACTAGTGC CACCGGCGGC CGCGCCGCCA CTACCGCCTT
            781 TATCGTCGTC ATCCTTGTAN TCAATGTCGT GNNCCTTGTN ATCCCCATCA TGGNCCTTAT
            841 AGNCCCNTCN GGCCTCCCTT CGACGCTNTT GGCGGNNNCN CANATAGATN GCCCNNGCNC
          901 CNNNNNNCAN NGGNGCCCCN ACAGCCNNGG NCNNNCNNNC ACNNANNNNN NNNNN
          //
          Primer: paGFP-M_R1 (Pr0893)
          Region: insert mid(GFP1-10 5',3XFLAG,Tom70(1-70))
          Sequence file: RDB16042_A8I3g.seq check
          >16042_16042_B2L8F_paGFP-M_R1_G01_07_ABI08.ab1
              1 NNNNNNNNNN NCNTGCCTTT TNNTGTGATC CGGNNAACGG GAAAAGCATT GAACACCATA
             61 GGTCAGAGTA GTGACAAGTG TTGGCCATGG AACAGGTAGT TTTCCAGTAG TGCAAATAAA
            121 TTTAAGTGTG AGTTTTCCGA TTGTAGCATC ACCTTCACCC TCTCCTCTGA CAGAAAATTT
            181 GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCAG TGAAAAGTTC
            241 TTCTCCTTTG CTCATACTAG TGCCACCGGC GGCCGCGCCG CCACTACCGC CTTTATCGTC
            301 GTCATCCTTG TAGTCAATGT CGTGATCCTT GTAATCCCCA TCATGGTCCT TATAGTCCCC
            361 TCTGGCCTCC CTTCGACGCT GTTGGCGGCT CCACAGATAG ATGGCCCCAG CTCCCAGGAG
            421 CAGTGGTGCC CCTACAGCCA GGGCCAACTG CCACCGAGGA AGGCCGCCTG TTCCGGGACC
            481 AGCGGTTCCT CCTCCACCCA CGCCTGACCC AGAGCTCGGC ACAGCGGCTG CAACGACGGC
            541 TGCTTCCACG GGTTTGGACG CGGCCATGGT GGCGGANCCG AGCTCGGTAC CAAGCTTAAG
            601 TTTAAACGCT AGCCAGCTTG NGTCTCCCTA TAGTGAGTCG NANTAATTTC NANAANCCAG
            661 TAAGCAGTGG GTTCTCTANT NAGCNNGANA NNTCTGCTNA NATAGNNCTC CNNCNNNANA
          721 CGCCNNNNNN CNNNTNNNNN NN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB16042_A8I3h.seq check
          >D05803_A6_A8I3_1_SV40pro_F_F08_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN ANNNNTGANG NNGNTTTTTT GGANGCCTAG GCTTTTGCAA
             61 AAAGCTCCCG GGNGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
            121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
            661 GCCCGACGGC GANGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
            781 TCANGACATA GCGTTGGCTA CNCGNNNTAT TGCTGAANAN CTTGGCGGCG ANNNNNCTGA
            841 CCGCTTCCTC NTGCTTTACG GTATCGCCGC NNCCNNNNNN CANCNCATCG CCNTCTANNG
          901 CCNNNNTNNN NNNNNCTTCT NNNCNGNNNN NNGGGGNNNN NNANNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl