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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Tom70N-FLAG-GFP1-10

Expression vector of human translocase of outer mitochondrial membrane 70 (Tom70), N-terminal segments 1-70.

Catalog number RDB16042
Resource name Tom70N-FLAG-GFP1-10
Alternative name pcDNA3.1-Tom70N-3xFLAG-GFP1-10 (pMM82)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 70 (Tom70), N-terminal segments 1-70 fused with 3xFLAG-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human TOMM70 cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
human TOMM70

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16042 Tom70N-FLAG-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16042_A8I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB16042_A8I3a.seq check
          >D05803_A6_A8I3_1_CMV-Forward_D08_11_ABI24.ab1
              1 CTTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CGAGCTCGGA
            181 TCCGCCACCA TGGCCGCGTC CAAACCCGTG GAAGCAGCCG TCGTTGCAGC CGCTGTGCCG
            241 AGCTCTGGGT CAGGCGTGGG TGGAGGAGGA ACCGCTGGTC CCGGAACAGG CGGCCTTCCT
            301 CGGTGGCAGT TGGCCCTGGC TGTAGGGGCA CCACTGCTCC TGGGAGCTGG GGCCATCTAT
            361 CTGTGGAGCC GCCAACAGCG TCGAAGGGAG GCCAGAGGGG ACTATAAGGA CCATGATGGG
            421 GATTACAAGG ATCACGACAT TGACTACAAG GATGACGACG ATAAAGGCGG TAGTGGCGGC
            481 GCGGCCGCCG GTGGCACTAG TATGAGCAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA
            541 ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG AGGAGAGGGT
            601 GAAGGTGATG CTACAATCGG AAAACTCACA CTTAAATTTA TTTGCACTAC TGGAAAACTA
            661 CCTGTTCCAT GGCCAACACT TGTCACTACT CTGACCTATG GTGTTCAATG CTTTTCCCGT
            721 TATCCGGATC ACATGAAAAG GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA
            781 CAGGAACGCA CTATATCTTT CAAAGATGAC GGGAAATACA AGACGCGTGC TGTAGTCAAG
            841 TTTGAAAGGT GATACCCTTG TTAATCGTAT CGAGTTAAAG GGTACTGATT TTTAAGAAGA
            901 TGGAAACATT CTCGGACACA AACTCGAGTA CACTTTTAAC TCACACAATG TATACATCAC
            961 GGGCAGACAA CAAAGATGGC ATCAAAGCTA ACCTTCACAG GTCGCCAACA CGGTTGGAGG
          1021 ATTGCATCCC GGTTCACTAG CAGATCGATA TCCACCGAAT ACTCCGAATT GC
          //
          Primer: BGH_rev2
          Sequence file: RDB16042_A8I3b.seq check
          >D05803_A6_A8I3_1_BGH_rev2_E08_14_ABI24.ab1
              1 TATAGTTAAG ATGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA GAAGGCACAG
             61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGATTA CTTTTCGTTG GGATCTTTCG
            121 AAAGGACAGT TTGTGTCGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG CCATCGCCAA
            181 TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGGA ACCATCTTCA ACGTTGTGGC
            241 GAACTGTGAA GTTAGCTTTG ATTCCATTCT TTTGTTTGTC TGCCGTGATG TATACATTGT
            301 GTGAGTTAAA GTTGTACTCG AGTTTGTGTC CGAGAATGTT TCCATCTTCT TTAAAATCAG
            361 TACCCTTTAA CTCGATACGA TTAACAAGGG TATCACCTTC AAACTTGACT ACAGCACGCG
            421 TCTTGTATTT CCCGTCATCT TTGAAAGATA TAGTGCGTTC CTGTACATAA CCTTCGGGCA
            481 TGGCACTCTT GAAAAAGTCA TGCCTTTTCA TGTGATCCGG ATAACGGGAA AAGCATTGAA
            541 CACCATAGGT CAGAGTAGTG ACAAGTGTTG GCCATGGAAC AGGTAGTTTT CCAGTAGTGC
            601 AAATAAATTT AAGTGTGAGT TTTCCGATTG TAGCATCACC TTCACCCTCT CCTCTGACAG
            661 AAAATTTGTG CCCATTAACA TCACCATCTA ATTCAACAAG AATTGGGACA ACTCCAGTGA
            721 AAAGTTCTTC TCCTTTGCTC ATACTAGTGC CACCGGCGGC CGCGCCGCCA CTACCGCCTT
            781 TATCGTCGTC ATCCTTGTAG TCAATGTCGT GATCCTTGTA ATCCCCATCA TGGTCCTTAT
            841 AGTCCCCTCT GGCCTCCCTT CGACGCTGTT GGCGGCTCCA CAGATAGATG GCCCCAGCTC
            901 CCAGGAGCAG TGGTGCCCCT ACAGCCAGGG CCCAACTGCC ACCGAGGAAG GCCGCCCTGT
            961 TCCCGGGACC CAGCGGGTTC CTCCCTCCAC CCACGCCCTG ACCCAGAGCT CGGCACAGCG
           1021 CTGCAACGGA CCGGCCTGCC TTTCCACGGG TTGACGGCGG TCATGCCTGG CCGAATTCGA
          1081 GTCTCGGTAC TAAGCTTAAG G
          //
          Primer: SV40pro_F
          Sequence file: RDB16042_A8I3c.seq check
          >D05803_A6_A8I3_1_SV40pro_F_F08_17_ABI24.ab1
              1 CCCTGTCCGG GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
             61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
            121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
            661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
            961 GCCCAACCTG CCATCACGAG ATTTCGATTC CCACCGCCCG CCTTTCCTAT GAAGGGTTGG
           1021 GCCTTCGGAT TCGTTTTCCC GGGGACGCTG GCTGGTATGC ATCCTTCCCA GCCGACGGGG
          1081 GTATTCAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl