Please review the QC test results indicated by icon below as well as clone information before placing your order.

Tom70N-FLAG-GFP1-10

Expression vector of human translocase of outer mitochondrial membrane 70 (Tom70), N-terminal segments 1-70.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering	Please visit Information of Request for Distribution.  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16042_A8I3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB16042_A8I3a.seq
>D05803_A6_A8I3_1_CMV-Forward_D08_11_ABI24.ab1     1 CTTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC   121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CGAGCTCGGA   181 TCCGCCACCA TGGCCGCGTC CAAACCCGTG GAAGCAGCCG TCGTTGCAGC CGCTGTGCCG   241 AGCTCTGGGT CAGGCGTGGG TGGAGGAGGA ACCGCTGGTC CCGGAACAGG CGGCCTTCCT   301 CGGTGGCAGT TGGCCCTGGC TGTAGGGGCA CCACTGCTCC TGGGAGCTGG GGCCATCTAT   361 CTGTGGAGCC GCCAACAGCG TCGAAGGGAG GCCAGAGGGG ACTATAAGGA CCATGATGGG   421 GATTACAAGG ATCACGACAT TGACTACAAG GATGACGACG ATAAAGGCGG TAGTGGCGGC   481 GCGGCCGCCG GTGGCACTAG TATGAGCAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA   541 ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG AGGAGAGGGT   601 GAAGGTGATG CTACAATCGG AAAACTCACA CTTAAATTTA TTTGCACTAC TGGAAAACTA   661 CCTGTTCCAT GGCCAACACT TGTCACTACT CTGACCTATG GTGTTCAATG CTTTTCCCGT   721 TATCCGGATC ACATGAAAAG GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA   781 CAGGAACGCA CTATATCTTT CAAAGATGAC GGGAAATACA AGACGCGTGC TGTAGTCAAG   841 TTTGAAAGGT GATACCCTTG TTAATCGTAT CGAGTTAAAG GGTACTGATT TTTAAGAAGA   901 TGGAAACATT CTCGGACACA AACTCGAGTA CACTTTTAAC TCACACAATG TATACATCAC   961 GGGCAGACAA CAAAGATGGC ATCAAAGCTA ACCTTCACAG GTCGCCAACA CGGTTGGAGG 1021 ATTGCATCCC GGTTCACTAG CAGATCGATA TCCACCGAAT ACTCCGAATT GC //
Primer: BGH_rev2 Sequence file: RDB16042_A8I3b.seq
>D05803_A6_A8I3_1_BGH_rev2_E08_14_ABI24.ab1     1 TATAGTTAAG ATGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGATTA CTTTTCGTTG GGATCTTTCG   121 AAAGGACAGT TTGTGTCGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG CCATCGCCAA   181 TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGGA ACCATCTTCA ACGTTGTGGC   241 GAACTGTGAA GTTAGCTTTG ATTCCATTCT TTTGTTTGTC TGCCGTGATG TATACATTGT   301 GTGAGTTAAA GTTGTACTCG AGTTTGTGTC CGAGAATGTT TCCATCTTCT TTAAAATCAG   361 TACCCTTTAA CTCGATACGA TTAACAAGGG TATCACCTTC AAACTTGACT ACAGCACGCG   421 TCTTGTATTT CCCGTCATCT TTGAAAGATA TAGTGCGTTC CTGTACATAA CCTTCGGGCA   481 TGGCACTCTT GAAAAAGTCA TGCCTTTTCA TGTGATCCGG ATAACGGGAA AAGCATTGAA   541 CACCATAGGT CAGAGTAGTG ACAAGTGTTG GCCATGGAAC AGGTAGTTTT CCAGTAGTGC   601 AAATAAATTT AAGTGTGAGT TTTCCGATTG TAGCATCACC TTCACCCTCT CCTCTGACAG   661 AAAATTTGTG CCCATTAACA TCACCATCTA ATTCAACAAG AATTGGGACA ACTCCAGTGA   721 AAAGTTCTTC TCCTTTGCTC ATACTAGTGC CACCGGCGGC CGCGCCGCCA CTACCGCCTT   781 TATCGTCGTC ATCCTTGTAG TCAATGTCGT GATCCTTGTA ATCCCCATCA TGGTCCTTAT   841 AGTCCCCTCT GGCCTCCCTT CGACGCTGTT GGCGGCTCCA CAGATAGATG GCCCCAGCTC   901 CCAGGAGCAG TGGTGCCCCT ACAGCCAGGG CCCAACTGCC ACCGAGGAAG GCCGCCCTGT   961 TCCCGGGACC CAGCGGGTTC CTCCCTCCAC CCACGCCCTG ACCCAGAGCT CGGCACAGCG  1021 CTGCAACGGA CCGGCCTGCC TTTCCACGGG TTGACGGCGG TCATGCCTGG CCGAATTCGA 1081 GTCTCGGTAC TAAGCTTAAG G //
Primer: SV40pro_F Sequence file: RDB16042_A8I3c.seq
>D05803_A6_A8I3_1_SV40pro_F_F08_17_ABI24.ab1     1 CCCTGTCCGG GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC   121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT   661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG   901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC   961 GCCCAACCTG CCATCACGAG ATTTCGATTC CCACCGCCCG CCTTTCCTAT GAAGGGTTGG  1021 GCCTTCGGAT TCGTTTTCCC GGGGACGCTG GCTGGTATGC ATCCTTCCCA GCCGACGGGG 1081 GTATTCAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl