The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: mitochondria.
Alternative name | pSFL75, pRS314-GPDp-Tom71-V5-GFP11 |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS314 (plasmid) |
Selectable markers | Amp^r (E. coli), TRP1 |
Gene/insert name | S. cerevisiae TOM71 cDNA |
Depositor|Developer | Tamura, Yasushi | |
Sequence (full) ![]() |
RDB16039zzt01.seq compiled by us |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16039 | Tom71-V5-GFP11 | DNA solution |
Materials & Methods section:
The Tom71-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16039). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16039_A8I3p1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB16039_A8I3e.seq ![]() |
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>16039_16039_A8I3F_HB-NOS_R_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NNNNNNNGGN NNNNCCNGGG TTTTCCCAGT CACGACGTTG 61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT 121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC 181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA 241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC 301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA 361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA 421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT 481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC 541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA 601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG 661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT 721 AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGNCGCAT 781 GGCCGANNAC TCCCTCCTGA NGTTTATCAC CAAGAACAAA GTGGCCATCC TANCAACGGT 841 CTCTGCGGGG ANTGCTGCTG NGGGTGCTTA TGTTTACTAC CAGCAAATTA AGCAGCAGCN 901 ACAGCANCNA TTGNANNGGT ACNAAAGANN ACCNANNTCN AANNGANGNC TTTGCTGNNN 961 NAAATNNNNN NNNNN // |
Primer: SV40pA3'element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC ter) Sequence file: RDB16039_A8I3f.seq ![]() |
>D05803_A3_A8I3_2_SV40pA3'element Reverse_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNANGG ANNNGCNNGC AACGCGGCCT TTTNACGGTT CCTGGCCTTT 61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT 121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG 181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG 241 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC 301 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT 361 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT 421 GACCATGATT ACGCCAAGCT CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG 481 GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT TCAGTATAAT 541 GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA TAAATAACGT 601 TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGANC TAGACTTCAG GTTGTCTAAC 661 TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC 721 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTANGTAATG 781 CCTGCAGCAT TGACATATTC GTGTANANCC ATATGATCTC TGCTTGTGGA ACCACCACCA 841 CTTCCACCAG TTNNNNCNNA GCCNNAAANA NGGTTNGGNA NGGGGTTTCC NNNANCNNNN 901 NGGCNNANGN TNNNAGNNNN NNNTTNANNC NNNTAACNAN CNNNNNNNAN CNNNNNNTTN 961 NNNNNNNNN // |
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC ter, V5-GFP11, TOM71 3') Sequence file: RDB16039_A8I3g.seq ![]() |
>D05803_A3_A8I3_2_CYC1ter_R_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA 61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT AGGTAATGCC 121 TGCAGCATTG ACATATTCGT GTAGAACCAT ATGATCTCTG CTTGTGGAAC CACCACCACT 181 TCCACCAGTT GAGTCTAAGC CCAAAAGAGG GTTTGGTATG GGTTTCCCAG AACCTCCGGC 241 AGAGGATCCA AGCATGCCTT TAGCCCTATA ACGAGCTAAT GTTAGCTCCA TCTTTGCACT 301 TATGATTGGA TCAGCCCTCA AACGCTTTTG TATCTTTGCA GCTTCCGCGA AGGTAGTAGC 361 TTGAAGTTTT TCATCCATAG TTCTTGCTAA AATTGCAGAA TCTTCAAACA ATTCTATGGC 421 CTCGTCAATT TTTTCCATTT GTAATTTTAG TTGTGCTAAC CCGATCTTCG CTTGTTCAGA 481 TCGTGGATCT AATTCACAGG CTTTTGTCAG CAGCTTAATG GCTGCGTTGA ATTTCTCTTC 541 ATCCAACTGG GTTGGATCTT GAGAGGATTG TCTAGCTAAT ATAGTTGCTT TCCCAATCAA 601 GGGTCCTATA CCCACATGTA TTTTTTCTTG CACTTCTTCC AACCTTTTAG CAATATCATA 661 TTGTTTAATT GCAGTATCAA AATCGCCCCT ATCTGTCAAG ATTTCAGCGA AAAANGTGGG 721 CANCTCANGT AANGTAGGAA ACTTCAACTT TGTTTCATTG AAAAAAGCTT CANATTCGGT 781 AAATTTACCT TGCTTGTACA ACAAACAGGC TAATTGAATG TANGGNTAAA CATTTTTCAG 841 GGTTCAAGCT TNNNGCCTTT TGGAANTCTT CCTNNGNNTT TTTTATAATC TNGNANAATA 901 AANTACATCN GCCNNCGNNN NNNATANNNN NNNNNN // |
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB16039_A8I3h.seq ![]() |
>D05803_A3_A8I3_2_TRP1pro_F_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG GCNGCTNGGN NTNNGTCTGT TATTAATTTC NCAGGTAGTT 61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG 121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC 181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC 241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA 301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG 361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC 421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG 481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC 541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC 601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGANG TGTGGAGACA AATGGTGTAA 661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT 721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA 781 GNTGCGTAAG GANNANNTAC CGCATCANGA AATTGTAAGC GTTAATATTT TGTTAAAANT 841 CGCGNTNANT TTTTNGTTAA ATCAGCNNAT TTTTTTAANC NANANGCCNA AANCNNNNAA 901 NNCCCNTNNN AANNNNAAAG NNNNNNNCGA NNNNNGGNNN NNNNNNN // |
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, TOM71 5') Sequence file: RDB16039_A8I3i.seq ![]() |
>16039_16039_A8I3F_TDH3pro_F_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNTGNN TGNNNTTCTG TNAATCTATT TCTTAAACTT CTTAAATTCT 61 ACTTTTATAG TTAGTCTTTT TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC 121 GGCCGCATGG CCGAAAACTC CCTCCTGAGG TTTATCACCA AGAACAAAGT GGCCATCCTA 181 GCAACGGTCT CTGCGGGGAC TGCTGCTGTG GGTGCTTATG TTTACTACCA GCAAATTAAG 241 CAGCAGCAAC AGCAGCAATT GAAGGGTACA AAAGACAACC GACGTCAAAG TGAGGCCTTT 301 GCTGGTCAAA ATGAAGACGA GGCGGACTTA AAGGACGACG GCAGCGTCGT AAGTGGAAGT 361 AATAAGAGGA AGAAGAAGAA GAATAAACGC AAGAGGAACA ATAAGGCAAA ATCGGGAGAA 421 GGGTTTGACT ATCCCTCCTT GCCCAATGGC GAGCCTGATA TTGCCCAATT GAAAGGCCTA 481 TCACCCTCCC AGAGACAAGC GTATGCCGTG CAACTGAAGA ACAGAGGTAA CCACTTCTTC 541 ACTGCTAAGA ATTTTAATGA GGCCATCAAA TACTACCAGT ATGCCATTGA GCTGGACCCG 601 AATGAACCAG TGTTCTATTC CAACATATCT GCTTGTTACA TTTCTACGGG CGACTTAGAG 661 AAGGTTATAG AGTTCACTAC AAAGGCACTT GAGATAAAAC CTGACCATTC TAAAGCCTTG 721 CTGAGACGTG CATCTGCCAA CGAGTCACTG GGCAACTTCA CTGATGCTAT GTTTGATCTA 781 TCTGTTTTGT CATTGAATGG TGACTTTGAT GGANCCTCCA TTGAACCTAT GCTGGAAAGA 841 NNTTTAAATA AGCAAGCAAT GAAAGTACTA AATGAAAACC NATCGAANGA TGAANGCAGA 901 NGCTCGCANN TTTTNGCCNN CNNACNCNTC NNNGNNNNCN TTTTTTTNGG GNANTTTTTN 961 NNNNNNNACN TNNNNANNNT TNNNNNNNNN N // |
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB16039_A8I3j.seq ![]() |
>D05803_A3_A8I3_2_Amp_R_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNTNN NCANTTATCN GGGTTATTGT CTCATGAGCG GNNACATATT 61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA 181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT 241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT 301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG 361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC 421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT 481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT 541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT 601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT 661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG 721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ANGGTCACAG CTNGTCTGTA 781 AGCGGATGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA NCGGGNGTTG GCGGGNGTCN 841 GGGCTGGCTT AACTATGCGG CATCAGANCA GANTGTACTG ANANNGCNNC NTANATCACG 901 ANNTTACTAT ATATNTNATA TNNNNNNNNT TTNNNANNNN AGCNNCNNNN NNNNNGNNNN 961 NNTNNNNNNN NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
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