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Tom71-V5-GFP11

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Catalog number RDB16039
Resource name Tom71-V5-GFP11
Alternative name pRS314-GPDp-Tom71-V5-GFP11 (pSFL75)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae TOM71 cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae TOM71 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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RDB16039 Tom71-V5-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16039_A8I3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB16039_A8I3e.seq check
>16039_16039_A8I3F_HB-NOS_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNNNNNNGGN NNNNCCNGGG TTTTCCCAGT CACGACGTTG
   61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT
  121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC
  181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA
  241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC
  301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA
  361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA
  421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT
  481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC
  541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA
  601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG
  661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT
  721 AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGNCGCAT
  781 GGCCGANNAC TCCCTCCTGA NGTTTATCAC CAAGAACAAA GTGGCCATCC TANCAACGGT
  841 CTCTGCGGGG ANTGCTGCTG NGGGTGCTTA TGTTTACTAC CAGCAAATTA AGCAGCAGCN
  901 ACAGCANCNA TTGNANNGGT ACNAAAGANN ACCNANNTCN AANNGANGNC TTTGCTGNNN
961 NAAATNNNNN NNNNN
//
Primer: SV40pA3'element Reverse
Region: T3 pro, insert 3' (CYC ter)
Sequence file: RDB16039_A8I3f.seq check
>D05803_A3_A8I3_2_SV40pA3'element Reverse_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNANGG ANNNGCNNGC AACGCGGCCT TTTNACGGTT CCTGGCCTTT
   61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
  121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
  181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
  241 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
  301 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT
  361 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT
  421 GACCATGATT ACGCCAAGCT CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG
  481 GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT TCAGTATAAT
  541 GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA TAAATAACGT
  601 TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGANC TAGACTTCAG GTTGTCTAAC
  661 TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC
  721 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTANGTAATG
  781 CCTGCAGCAT TGACATATTC GTGTANANCC ATATGATCTC TGCTTGTGGA ACCACCACCA
  841 CTTCCACCAG TTNNNNCNNA GCCNNAAANA NGGTTNGGNA NGGGGTTTCC NNNANCNNNN
  901 NGGCNNANGN TNNNAGNNNN NNNTTNANNC NNNTAACNAN CNNNNNNNAN CNNNNNNTTN
961 NNNNNNNNN
//
Primer: CYC1ter_R
Region: insert mid (CYC ter, V5-GFP11, TOM71 3')
Sequence file: RDB16039_A8I3g.seq check
>D05803_A3_A8I3_2_CYC1ter_R_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT AGGTAATGCC
  121 TGCAGCATTG ACATATTCGT GTAGAACCAT ATGATCTCTG CTTGTGGAAC CACCACCACT
  181 TCCACCAGTT GAGTCTAAGC CCAAAAGAGG GTTTGGTATG GGTTTCCCAG AACCTCCGGC
  241 AGAGGATCCA AGCATGCCTT TAGCCCTATA ACGAGCTAAT GTTAGCTCCA TCTTTGCACT
  301 TATGATTGGA TCAGCCCTCA AACGCTTTTG TATCTTTGCA GCTTCCGCGA AGGTAGTAGC
  361 TTGAAGTTTT TCATCCATAG TTCTTGCTAA AATTGCAGAA TCTTCAAACA ATTCTATGGC
  421 CTCGTCAATT TTTTCCATTT GTAATTTTAG TTGTGCTAAC CCGATCTTCG CTTGTTCAGA
  481 TCGTGGATCT AATTCACAGG CTTTTGTCAG CAGCTTAATG GCTGCGTTGA ATTTCTCTTC
  541 ATCCAACTGG GTTGGATCTT GAGAGGATTG TCTAGCTAAT ATAGTTGCTT TCCCAATCAA
  601 GGGTCCTATA CCCACATGTA TTTTTTCTTG CACTTCTTCC AACCTTTTAG CAATATCATA
  661 TTGTTTAATT GCAGTATCAA AATCGCCCCT ATCTGTCAAG ATTTCAGCGA AAAANGTGGG
  721 CANCTCANGT AANGTAGGAA ACTTCAACTT TGTTTCATTG AAAAAAGCTT CANATTCGGT
  781 AAATTTACCT TGCTTGTACA ACAAACAGGC TAATTGAATG TANGGNTAAA CATTTTTCAG
  841 GGTTCAAGCT TNNNGCCTTT TGGAANTCTT CCTNNGNNTT TTTTATAATC TNGNANAATA
901 AANTACATCN GCCNNCGNNN NNNATANNNN NNNNNN
//
Primer: TRP1pro_F
Region: TRP1
Sequence file: RDB16039_A8I3h.seq check
>D05803_A3_A8I3_2_TRP1pro_F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GCNGCTNGGN NTNNGTCTGT TATTAATTTC NCAGGTAGTT
   61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG
  121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC
  181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC
  241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA
  301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG
  361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC
  421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG
  481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC
  541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC
  601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGANG TGTGGAGACA AATGGTGTAA
  661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT
  721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA
  781 GNTGCGTAAG GANNANNTAC CGCATCANGA AATTGTAAGC GTTAATATTT TGTTAAAANT
  841 CGCGNTNANT TTTTNGTTAA ATCAGCNNAT TTTTTTAANC NANANGCCNA AANCNNNNAA
901 NNCCCNTNNN AANNNNAAAG NNNNNNNCGA NNNNNGGNNN NNNNNNN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, TOM71 5')
Sequence file: RDB16039_A8I3i.seq check
>16039_16039_A8I3F_TDH3pro_F_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTGNN TGNNNTTCTG TNAATCTATT TCTTAAACTT CTTAAATTCT
   61 ACTTTTATAG TTAGTCTTTT TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC
  121 GGCCGCATGG CCGAAAACTC CCTCCTGAGG TTTATCACCA AGAACAAAGT GGCCATCCTA
  181 GCAACGGTCT CTGCGGGGAC TGCTGCTGTG GGTGCTTATG TTTACTACCA GCAAATTAAG
  241 CAGCAGCAAC AGCAGCAATT GAAGGGTACA AAAGACAACC GACGTCAAAG TGAGGCCTTT
  301 GCTGGTCAAA ATGAAGACGA GGCGGACTTA AAGGACGACG GCAGCGTCGT AAGTGGAAGT
  361 AATAAGAGGA AGAAGAAGAA GAATAAACGC AAGAGGAACA ATAAGGCAAA ATCGGGAGAA
  421 GGGTTTGACT ATCCCTCCTT GCCCAATGGC GAGCCTGATA TTGCCCAATT GAAAGGCCTA
  481 TCACCCTCCC AGAGACAAGC GTATGCCGTG CAACTGAAGA ACAGAGGTAA CCACTTCTTC
  541 ACTGCTAAGA ATTTTAATGA GGCCATCAAA TACTACCAGT ATGCCATTGA GCTGGACCCG
  601 AATGAACCAG TGTTCTATTC CAACATATCT GCTTGTTACA TTTCTACGGG CGACTTAGAG
  661 AAGGTTATAG AGTTCACTAC AAAGGCACTT GAGATAAAAC CTGACCATTC TAAAGCCTTG
  721 CTGAGACGTG CATCTGCCAA CGAGTCACTG GGCAACTTCA CTGATGCTAT GTTTGATCTA
  781 TCTGTTTTGT CATTGAATGG TGACTTTGAT GGANCCTCCA TTGAACCTAT GCTGGAAAGA
  841 NNTTTAAATA AGCAAGCAAT GAAAGTACTA AATGAAAACC NATCGAANGA TGAANGCAGA
  901 NGCTCGCANN TTTTNGCCNN CNNACNCNTC NNNGNNNNCN TTTTTTTNGG GNANTTTTTN
961 NNNNNNNACN TNNNNANNNT TNNNNNNNNN N
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB16039_A8I3j.seq check
>D05803_A3_A8I3_2_Amp_R_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN NCANTTATCN GGGTTATTGT CTCATGAGCG GNNACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
  181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
  241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
  301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
  361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
  421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
  481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
  541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
  601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
  661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
  721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ANGGTCACAG CTNGTCTGTA
  781 AGCGGATGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA NCGGGNGTTG GCGGGNGTCN
  841 GGGCTGGCTT AACTATGCGG CATCAGANCA GANTGTACTG ANANNGCNNC NTANATCACG
  901 ANNTTACTAT ATATNTNATA TNNNNNNNNT TTNNNANNNN AGCNNCNNNN NNNNNGNNNN
961 NNTNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2021.01.06

GNP_filter3_RDBDEP_html_201222.pl