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Tom71-V5-GFP11

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Catalog number RDB16039
Resource name Tom71-V5-GFP11
Alternative name pRS314-GPDp-Tom71-V5-GFP11 (pSFL75)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae TOM71 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae TOM71

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16039 Tom71-V5-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16039zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16039_A8I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro)
          Sequence file: RDB16039_A8I3e.seq check
          >16039_16039_A8I3F_HB-NOS_R_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NNNNNNNGGN NNNNCCNGGG TTTTCCCAGT CACGACGTTG
             61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT
            121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC
            181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA
            241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC
            301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA
            361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA
            421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT
            481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC
            541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA
            601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG
            661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT
            721 AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGNCGCAT
            781 GGCCGANNAC TCCCTCCTGA NGTTTATCAC CAAGAACAAA GTGGCCATCC TANCAACGGT
            841 CTCTGCGGGG ANTGCTGCTG NGGGTGCTTA TGTTTACTAC CAGCAAATTA AGCAGCAGCN
            901 ACAGCANCNA TTGNANNGGT ACNAAAGANN ACCNANNTCN AANNGANGNC TTTGCTGNNN
          961 NAAATNNNNN NNNNN
          //
          Primer: SV40pA3'element Reverse
          Region: T3 pro, insert 3' (CYC ter)
          Sequence file: RDB16039_A8I3f.seq check
          >D05803_A3_A8I3_2_SV40pA3'element Reverse_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNANGG ANNNGCNNGC AACGCGGCCT TTTNACGGTT CCTGGCCTTT
             61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
            121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
            181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
            241 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
            301 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT
            361 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT
            421 GACCATGATT ACGCCAAGCT CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG
            481 GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT TCAGTATAAT
            541 GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA TAAATAACGT
            601 TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGANC TAGACTTCAG GTTGTCTAAC
            661 TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC
            721 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTANGTAATG
            781 CCTGCAGCAT TGACATATTC GTGTANANCC ATATGATCTC TGCTTGTGGA ACCACCACCA
            841 CTTCCACCAG TTNNNNCNNA GCCNNAAANA NGGTTNGGNA NGGGGTTTCC NNNANCNNNN
            901 NGGCNNANGN TNNNAGNNNN NNNTTNANNC NNNTAACNAN CNNNNNNNAN CNNNNNNTTN
          961 NNNNNNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC ter, V5-GFP11, TOM71 3')
          Sequence file: RDB16039_A8I3g.seq check
          >D05803_A3_A8I3_2_CYC1ter_R_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA
             61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT AGGTAATGCC
            121 TGCAGCATTG ACATATTCGT GTAGAACCAT ATGATCTCTG CTTGTGGAAC CACCACCACT
            181 TCCACCAGTT GAGTCTAAGC CCAAAAGAGG GTTTGGTATG GGTTTCCCAG AACCTCCGGC
            241 AGAGGATCCA AGCATGCCTT TAGCCCTATA ACGAGCTAAT GTTAGCTCCA TCTTTGCACT
            301 TATGATTGGA TCAGCCCTCA AACGCTTTTG TATCTTTGCA GCTTCCGCGA AGGTAGTAGC
            361 TTGAAGTTTT TCATCCATAG TTCTTGCTAA AATTGCAGAA TCTTCAAACA ATTCTATGGC
            421 CTCGTCAATT TTTTCCATTT GTAATTTTAG TTGTGCTAAC CCGATCTTCG CTTGTTCAGA
            481 TCGTGGATCT AATTCACAGG CTTTTGTCAG CAGCTTAATG GCTGCGTTGA ATTTCTCTTC
            541 ATCCAACTGG GTTGGATCTT GAGAGGATTG TCTAGCTAAT ATAGTTGCTT TCCCAATCAA
            601 GGGTCCTATA CCCACATGTA TTTTTTCTTG CACTTCTTCC AACCTTTTAG CAATATCATA
            661 TTGTTTAATT GCAGTATCAA AATCGCCCCT ATCTGTCAAG ATTTCAGCGA AAAANGTGGG
            721 CANCTCANGT AANGTAGGAA ACTTCAACTT TGTTTCATTG AAAAAAGCTT CANATTCGGT
            781 AAATTTACCT TGCTTGTACA ACAAACAGGC TAATTGAATG TANGGNTAAA CATTTTTCAG
            841 GGTTCAAGCT TNNNGCCTTT TGGAANTCTT CCTNNGNNTT TTTTATAATC TNGNANAATA
          901 AANTACATCN GCCNNCGNNN NNNATANNNN NNNNNN
          //
          Primer: TRP1pro_F
          Region: TRP1
          Sequence file: RDB16039_A8I3h.seq check
          >D05803_A3_A8I3_2_TRP1pro_F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG GCNGCTNGGN NTNNGTCTGT TATTAATTTC NCAGGTAGTT
             61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG
            121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC
            181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC
            241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA
            301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG
            361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC
            421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG
            481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC
            541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC
            601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGANG TGTGGAGACA AATGGTGTAA
            661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT
            721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA
            781 GNTGCGTAAG GANNANNTAC CGCATCANGA AATTGTAAGC GTTAATATTT TGTTAAAANT
            841 CGCGNTNANT TTTTNGTTAA ATCAGCNNAT TTTTTTAANC NANANGCCNA AANCNNNNAA
          901 NNCCCNTNNN AANNNNAAAG NNNNNNNCGA NNNNNGGNNN NNNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, TOM71 5')
          Sequence file: RDB16039_A8I3i.seq check
          >16039_16039_A8I3F_TDH3pro_F_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTGNN TGNNNTTCTG TNAATCTATT TCTTAAACTT CTTAAATTCT
             61 ACTTTTATAG TTAGTCTTTT TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC
            121 GGCCGCATGG CCGAAAACTC CCTCCTGAGG TTTATCACCA AGAACAAAGT GGCCATCCTA
            181 GCAACGGTCT CTGCGGGGAC TGCTGCTGTG GGTGCTTATG TTTACTACCA GCAAATTAAG
            241 CAGCAGCAAC AGCAGCAATT GAAGGGTACA AAAGACAACC GACGTCAAAG TGAGGCCTTT
            301 GCTGGTCAAA ATGAAGACGA GGCGGACTTA AAGGACGACG GCAGCGTCGT AAGTGGAAGT
            361 AATAAGAGGA AGAAGAAGAA GAATAAACGC AAGAGGAACA ATAAGGCAAA ATCGGGAGAA
            421 GGGTTTGACT ATCCCTCCTT GCCCAATGGC GAGCCTGATA TTGCCCAATT GAAAGGCCTA
            481 TCACCCTCCC AGAGACAAGC GTATGCCGTG CAACTGAAGA ACAGAGGTAA CCACTTCTTC
            541 ACTGCTAAGA ATTTTAATGA GGCCATCAAA TACTACCAGT ATGCCATTGA GCTGGACCCG
            601 AATGAACCAG TGTTCTATTC CAACATATCT GCTTGTTACA TTTCTACGGG CGACTTAGAG
            661 AAGGTTATAG AGTTCACTAC AAAGGCACTT GAGATAAAAC CTGACCATTC TAAAGCCTTG
            721 CTGAGACGTG CATCTGCCAA CGAGTCACTG GGCAACTTCA CTGATGCTAT GTTTGATCTA
            781 TCTGTTTTGT CATTGAATGG TGACTTTGAT GGANCCTCCA TTGAACCTAT GCTGGAAAGA
            841 NNTTTAAATA AGCAAGCAAT GAAAGTACTA AATGAAAACC NATCGAANGA TGAANGCAGA
            901 NGCTCGCANN TTTTNGCCNN CNNACNCNTC NNNGNNNNCN TTTTTTTNGG GNANTTTTTN
          961 NNNNNNNACN TNNNNANNNT TNNNNNNNNN N
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB16039_A8I3j.seq check
          >D05803_A3_A8I3_2_Amp_R_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTNN NCANTTATCN GGGTTATTGT CTCATGAGCG GNNACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
            181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
            241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
            301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
            361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
            421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
            481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
            541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
            601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
            661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
            721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ANGGTCACAG CTNGTCTGTA
            781 AGCGGATGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA NCGGGNGTTG GCGGGNGTCN
            841 GGGCTGGCTT AACTATGCGG CATCAGANCA GANTGTACTG ANANNGCNNC NTANATCACG
            901 ANNTTACTAT ATATNTNATA TNNNNNNNNT TTNNNANNNN AGCNNCNNNN NNNNNGNNNN
          961 NNTNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl