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Tom71-V5-GFP11

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Catalog number RDB16039
Resource name Tom71-V5-GFP11
Alternative name pRS314-GPDp-Tom71-V5-GFP11 (pSFL75)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae TOM71 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae TOM71 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB16039 Tom71-V5-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16039_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB16039_A8I3a.seq check
>D05803_A3_A8I3_2_M13_-40_C10_07_ABI24.ab1
    1 AACCGAGTCA TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT ATCATTATCA
   61 ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC TAACTTTATT
  121 TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA ATAGGGGGCG
  181 GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC CTGGCATCCA
  241 CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA ATCCCAGCAC
  301 CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA GCGCAACTAC
  361 AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT GATGCAACCT
  421 GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC TCATTTTCTT
  481 ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA AAGGTTGAAA
  541 CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG GTAGGTATTG
  601 ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT AGTTAGTCTT
  661 TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGCCGCAT GGCCGAAAAC
  721 TCCCTCCTGA GGTTTATCAC CAAGAACAAA GTGGCCATCC TAGCAACGGT CTCTGCGGGG
  781 ACTGCTGCTG TGGGTGCTTA TGTTTACTAC CAGCAAATTA AGCAGCAGCA ACAGCAGCAA
  841 TTGAAGGGTA CAAAAGACAA CCGACGTCAA AGTGAGGCCT TTGCTGGTCA AAATGAAGAC
  901 GAGGCGGACT TAAAGGACGA CGGCAGCGTC GTAAGTGGAA GTAATAAGAG GAAGAAGAAG
  961 AGATAAACGC AAGAGGAACA ATAATGCAAA ATCGGGAGAA CGTTTGACCT ATCCCTCGTT
 1021 GCCCAATGAC GAAGCTTGAT ATTGGCCCAA GTGAACGGAC CTATCACCCT TCCAGCGACC
1081 AAGGCGGTAT TGC
//
Primer: CYC1ter_R
Sequence file: RDB16039_A8I3b.seq check
>D05803_A3_A8I3_2_CYC1ter_R_C01_07_ABI24.ab1
    1 ATTTCCGTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT AGGTAATGCC
  121 TGCAGCATTG ACATATTCGT GTAGAACCAT ATGATCTCTG CTTGTGGAAC CACCACCACT
  181 TCCACCAGTT GAGTCTAAGC CCAAAAGAGG GTTTGGTATG GGTTTCCCAG AACCTCCGGC
  241 AGAGGATCCA AGCATGCCTT TAGCCCTATA ACGAGCTAAT GTTAGCTCCA TCTTTGCACT
  301 TATGATTGGA TCAGCCCTCA AACGCTTTTG TATCTTTGCA GCTTCCGCGA AGGTAGTAGC
  361 TTGAAGTTTT TCATCCATAG TTCTTGCTAA AATTGCAGAA TCTTCAAACA ATTCTATGGC
  421 CTCGTCAATT TTTTCCATTT GTAATTTTAG TTGTGCTAAC CCGATCTTCG CTTGTTCAGA
  481 TCGTGGATCT AATTCACAGG CTTTTGTCAG CAGCTTAATG GCTGCGTTGA ATTTCTCTTC
  541 ATCCAACTGG GTTGGATCTT GAGAGGATTG TCTAGCTAAT ATAGTTGCTT TCCCAATCAA
  601 GGGTCCTATA CCCACATGTA TTTTTTCTTG CACTTCTTCC AACCTTTTAG CAATATCATA
  661 TTGTTTAATT GCAGTATCAA AATCGCCCCT ATCTGTCAAG ATTTCAGCGA AAAAGGTGGG
  721 CACCTCAGGT AAGGTAGGAA ACTTCAACTT TGTTTCATTG AAAAAAGCTT CAGATTCGGT
  781 AAATTTACCT TGCTTGTACA ACAAACAGGC TAATTGAATG TAGGGATAAA CATTTTTCAG
  841 GGTTCAAGCT TTGGGCCTTT TGGAAATCTT CCTTGGCATT TTTTATAATC TTGTAGAATA
  901 AAATACATCT GCCCACGGTG GTAATAGGTT GGCGGATACT CAGGGTTTAG ATCAACAGCT
  961 TTTTTTGGAA AAAATTTGAA AAAATTTCTT TGAGAGTTTT TCTTTATCGG CCCACGTCCA
 1021 ATGCCCAAAA AATATGGTAC GAGTTTGGTG GTGGATGCAG CTTATAGATC CTTGCTAAAG
1081 TACTGGTCAA TCTAACC
//
Primer: TRP1pro_F
Sequence file: RDB16039_A8I3c.seq check
>D05803_A3_A8I3_2_TRP1pro_F_C02_08_ABI24.ab1
    1 GCATGGCATT AGCAACAAAG GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT
   61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG
  121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC
  181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC
  241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA
  301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG
  361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC
  421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG
  481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC
  541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC
  601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA
  661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT
  721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA
  781 GATGCGTAAG GAGAAAATAC CGCATCAGGA AATTGTAAGC GTTAATATTT TGTTAAAATT
  841 CGCGTTAAAT TTTTTGTTAA ATCAGCTCAT TTTTTTAACC AATAGGCCGA AATCGGCAAA
  901 ATCCCTTTAT AAATCAAAAG AATAGACCGA GATAGGGGTT GAGTGTTGTT CCAGTTTGGA
  961 ACAAGAGTCC ACTATTAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CGTCTATCAA
 1021 GGTCGATGGA CCCACTACGT GATCAATCAC CTATCAAGCT TTTTTTGGGG GATCGAAGGT
1081 GCCATTTAAA GTCAT
//
Primer: Amp_R
Sequence file: RDB16039_A8I3d.seq check
>D05803_A3_A8I3_2_Amp_R_D02_11_ABI24.ab1
    1 TTGGTTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
  181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
  241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
  301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
  361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
  421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
  481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
  541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
  601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
  661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
  721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA
  781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG
  841 GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATAGATCACG
  901 ACATTACTAT ATATATAATA TAGGAAGCAT TTAATAGAAC AGCATCGTAA TATATGTGTA
  961 CTTTGCAGTT ATGACGCCAG ATGGCAGTAG TGGAGATATT CTTTTATTGA AAATAGCTGT
 1021 CACTACGTAC AATCATGGAT TCGGAGCCTA TCTTTTTTGC CGATTAGAAT CAATCCGGTT
1081 CGGACAAAGA AAAAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl