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Tom71-GFP11

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Catalog number RDB16038
Resource name Tom71-GFP11
Alternative name pRS314-GPDp-Tom71-GFP11 (pSFL23)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae TOM71 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae TOM71 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB16038 Tom71-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16038_A8I3p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Region: insert5'(GPD pro,TOM71 5')
Sequence file: RDB16038_A8I3f.seq check
>D05803_A2_A8I3_1_M13_-40_B10_04_ABI24.ab1
    1 AACAACTAAT TGTATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA TCATTATCAA
   61 TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT AACTTTATTT
  121 AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA TAGGGGGCGG
  181 GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC TGGCATCCAC
  241 TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA TCCCAGCACC
  301 AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG CGCAACTACA
  361 GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG ATGCAACCTG
  421 CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT CATTTTCTTA
  481 CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA AGGTTGAAAC
  541 CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG TAGGTATTGA
  601 TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA GTTAGTCTTT
  661 TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCATG GCCGAAAACT
  721 CCCTCCTGAG GTTTATCACC AAGAACAAAG TGGCCATCCT AGCAACGGTC TCTGCGGGGA
  781 CTGCTGCTGT GGGTGCTTAT GTTTACTACC AGCAAATTAA GCAGCAGCAA CAGCAGCAAT
  841 TGAAGGGTAC AAAAGACAAC CGACGTCAAA GTGAGGCCTT TGCTGGTCAA AATGAAGACG
  901 AGGCGGACTT AAAGGACGAC GGCAGCGTCG TAAGTGGAAA GTAATAAGAG GAAGAAGAAG
  961 AAGAATAAAC GCAAGAGGAA ACAATAACGC AAAATCGGGA GAAGGTTTGA CTATCCTCCC
 1021 TTGCCCAATG GCCGAGCCTG GATATTGCCC CAAGTGAACG ACTAATCACC CTTCCCAGAA
1081 GAAACAAGCG GTTAATTC
//
Primer: SV40pA3'element Reverse
Region: insert3'(CYC1 ter,GFP11)
Sequence file: RDB16038_A8I3g.seq check
>16038_16038_A9LbF_SV40pA3'elementReverse_A03_03_ABI24.ab1
    1 NNNNNNNNNG GNNNNNNNNA NGGNNAANGC NNGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
  301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
  361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
  421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
  481 ACCGGCCGCA AATTAAAGCC TTCGAGCGTC CCAAAACCTT CTCAAGCAAG GTTTTCAGTA
  541 TAATGTTACA TGCGTACACG CGTCTGTACA GAAAAAAAAG AAAAATTTGA AATATAAATA
  601 ACGTTCTTAA TACTAACATA ACTATAAAAA AATAAATAGG GACCTAGACT TCAGGTTGTC
  661 TAACTCCTTC CTTTTCGGTT AGAGCGGATG TGGGGGGAGG GCGTGAATGT AAGCGTGACA
  721 TAACTAATTA CATGACTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCTTATGT
  781 AATCCCAGCA GCATTTACGT ACTCATGAAG GANCATGTGG TCACGANTTG TTGATCCGCC
  841 NCCAGANCCT CCGGATNCAA GCATGCCTTT AGCCCNATAA CGANCNAANG TNANCTNCNT
  901 CTTTGCACTN ATGATNNGAT CAGCCCNCAA ACGCTTTTGT ANNNTNNCNG CTNCNNNGAA
961 NNNTANNANC NNGNANNTTT TNCNTCCNNA NNNNTNGNNN AANNTNNNN
//
Primer: TRP1pro_F
Region: TRP1 pro,TRP1
Sequence file: RDB16038_A8I3h.seq check
>D05803_A2_A8I3_1_TRP1pro_F_G01_19_ABI24.ab1
    1 GACTGGGATT AGCAACAAGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA CAGGTAGTTC
   61 TGGTCCATTG GTGAAAGTTT GCGGCTTGCA GAGCACAGAG GCCGCAGAAT GTGCTCTAGA
  121 TTCCGATGCT GACTTGCTGG GTATTATATG TGTGCCCAAT AGAAAGAGAA CAATTGACCC
  181 GGTTATTGCA AGGAAAATTT CAAGTCTTGT AAAAGCATAT AAAAATAGTT CAGGCACTCC
  241 GAAATACTTG GTTGGCGTGT TTCGTAATCA ACCTAAGGAG GATGTTTTGG CTCTGGTCAA
  301 TGATTACGGC ATTGATATCG TCCAACTGCA TGGAGATGAG TCGTGGCAAG AATACCAAGA
  361 GTTCCTCGGT TTGCCAGTTA TTAAAAGACT CGTATTTCCA AAAGACTGCA ACATACTACT
  421 CAGTGCAGCT TCACAGAAAC CTCATTCGTT TATTCCCTTG TTTGATTCAG AAGCAGGTGG
  481 GACAGGTGAA CTTTTGGATT GGAACTCGAT TTCTGACTGG GTTGGAAGGC AAGAGAGCCC
  541 CGAAAGCTTA CATTTTATGT TAGCTGGTGG ACTGACGCCA GAAAATGTTG GTGATGCGCT
  601 TAGATTAAAT GGCGTTATTG GTGTTGATGT AAGCGGAGGT GTGGAGACAA ATGGTGTAAA
  661 AGACTCTAAC AAAATAGCAA ATTTCGTCAA AAATGCTAAG AAATAGGTTA TTACTGAGTA
  721 GTATTTATTT AAGTATTGTT TGTGCACTTG CCGATCTATG CGGTGTGAAA TACCGCACAG
  781 ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAGCG TTAATATTTT GTTAAAATTC
  841 GCGTTAAATT TTTTGTTAAA TCAGCTCATT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
  901 TCCCTTTATA AATCAAAAGA AATAGACCGA GATAGGGTTT GAGTGTTTGT TTCCAGTTTG
  961 GAACAAGAGT CCACTATTTA AAGAACGGTG GTACCTCCAA CGGTCCAAAG GGCGAAAAAA
 1021 GTCGTCCTAT CATGATCGAT GGACCCAACT TACGTTGATC AATTCACCCT TATCAAGTTT
1081 TTTGGGGTTC GAGTGCCGGT AAAAG
//
Primer: CYC1ter_R
Region: insert3'(CYC1 ter,GFP11,TOM71 3')
Sequence file: RDB16038_A8I3i.seq check
>D05803_A2_A8I3_1_CYC1ter_R_B01_04_ABI24.ab1
    1 CTTTCGTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TATGTAATCC
  121 CAGCAGCATT TACGTACTCA TGAAGGACCA TGTGGTCACG ACTTGTTGAT CCGCCACCAG
  181 ACCCTCCGGA TCCAAGCATG CCTTTAGCCC TATAACGAGC TAATGTTAGC TCCATCTTTG
  241 CACTTATGAT TGGATCAGCC CTCAAACGCT TTTGTATCTT TGCAGCTTCC GCGAAGGTAG
  301 TAGCTTGAAG TTTTTCATCC ATAGTTCTTG CTAAAATTGC AGAATCTTCA AACAATTCTA
  361 TGGCCTCGTC AATTTTTTCC ATTTGTAATT TTAGTTGTGC TAACCCGATC TTCGCTTGTT
  421 CAGATCGTGG ATCTAATTCA CAGGCTTTTG TCAGCAGCTT AATGGCTGCG TTGAATTTCT
  481 CTTCATCCAA CTGGGTTGGA TCTTGAGAGG ATTGTCTAGC TAATATAGTT GCTTTCCCAA
  541 TCAAGGGTCC TATACCCACA TGTATTTTTT CTTGCACTTC TTCCAACCTT TTAGCAATAT
  601 CATATTGTTT AATTGCAGTA TCAAAATCGC CCCTATCTGT CAAGATTTCA GCGAAAAAGG
  661 TGGGCACCTC AGGTAAGGTA GGAAACTTCA ACTTTGTTTC ATTGAAAAAA GCTTCAGATT
  721 CGGTAAATTT ACCTTGCTTG TACAACAAAC AGGCTAATTG AATGTAGGGA TAAACATTTT
  781 CAGGGTTCAA GCTTTGGGCC TTTTGGAAAT CTTCCTTGGC ATTTTTATAA TCTTGTAGAA
  841 TAAAATACAT CTGCCCACGG TGGTAATAGG TTGGCGGATA CTCAGGGTTT AGATCAACAG
  901 CTTTTTTGGA AAAAATTTGA AAAAATTCTT GAGAGTTTTC TTTATCGGCC CAAGGTCAAT
  961 GCCCAAAAAT ATGTACGAAT TTGGGTGTTT GGATGCAGGT TTTATAGATT TCTTGTTAAA
 1021 GACTTGGGGC AATCCTACAG CTTATTTTTT AGAATGGAGA TTACCCGTGT AGCACATTGC
1081 TCAGGGGCCA
//
Primer: Amp_R
Region: AmpR,CEN_ARS
Sequence file: RDB16038_A8I3j.seq check
>D05803_A2_A8I3_1_Amp_R_H01_22_ABI24.ab1
    1 TTCTTTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
  781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GCGCGTCAGC GGTGTTGCGG TGTCGGGGCT
  841 GGCTTACTAT GCGGCATCAG AGCAGATTGT ACTGGAGAGT GCCACCATAG ATCACGACTT
  901 ACTATATATT ATAATATACG AGCATTATAG AACAGGCATC GTAATATATG TGTACTTGCA
  961 GGTTATGACG CGATGCAGTA ATGAAGATAT TCCTTAATGG AAAATAGCCT GGTCACCTTA
1021 CGTACAATCT TGAATCGCGA CTTACGTATA TGCCGATAGA ATCATTCGTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl