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Tom71-GFP11 (#RDB16038)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP11; location: mitochondria.

Alternative name pSFL23, pRS314-GPDp-Tom71-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae TOM71 cDNA
Depositor|Developer Tamura, Yasushi |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16038 Tom71-GFP11 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Tom71-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16038).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16038_A8I3p1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800)
Region: insert 5' (T7 pro, GPD pro)
Sequence file: RDB16038_A8I3k.seq check
>16038_16038_B0JcF_HB-NOS_R_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTGGGTNNN GCCNGGGTTT TCCCNGTCAC GACGTTGTAA
   61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC
  121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA
  181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA
  241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG
  301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC
  361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG
  421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT
  481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA
  541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG
  601 GNTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGANGGTA
  661 NGNANTGATT GNAATTCTGT AAATCTATTT CNTAAACTTC NTNANTTCTA CTTTTATAGT
721 TAGNCTTTTT TTTNNNNTTT NAAANNNNNN NNANNNNNNN NNN
//
Primer: SV40pA3'Element Reverse (Pr0108)
Region: insert 3' (T3 pro, CYC1 ter, GFP11)
Sequence file: RDB16038_A8I3l.seq check
>16038_16038_A9LbF_SV40pA3'elementReverse_A03_03_ABI24.ab1
    1 NNNNNNNNNG GNNNNNNNNA NGGNNAANGC NNGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
  301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
  361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
  421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
  481 ACCGGCCGCA AATTAAAGCC TTCGAGCGTC CCAAAACCTT CTCAAGCAAG GTTTTCAGTA
  541 TAATGTTACA TGCGTACACG CGTCTGTACA GAAAAAAAAG AAAAATTTGA AATATAAATA
  601 ACGTTCTTAA TACTAACATA ACTATAAAAA AATAAATAGG GACCTAGACT TCAGGTTGTC
  661 TAACTCCTTC CTTTTCGGTT AGAGCGGATG TGGGGGGAGG GCGTGAATGT AAGCGTGACA
  721 TAACTAATTA CATGACTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCTTATGT
  781 AATCCCAGCA GCATTTACGT ACTCATGAAG GANCATGTGG TCACGANTTG TTGATCCGCC
  841 NCCAGANCCT CCGGATNCAA GCATGCCTTT AGCCCNATAA CGANCNAANG TNANCTNCNT
  901 CTTTGCACTN ATGATNNGAT CAGCCCNCAA ACGCTTTTGT ANNNTNNCNG CTNCNNNGAA
961 NNNTANNANC NNGNANNTTT TNCNTCCNNA NNNNTNGNNN AANNTNNNNN NNNNN
//
Primer: CYC1ter_R (Pr0022)
Region: insert mid (CYC1 ter, GFP11, TOM71 3')
Sequence file: RDB16038_A8I3m.seq check
>D05803_A2_A8I3_1_CYC1ter_R_B01_04_ABI24.ab1
    1 CTTTCGTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TATGTAATCC
  121 CAGCAGCATT TACGTACTCA TGAAGGACCA TGTGGTCACG ACTTGTTGAT CCGCCACCAG
  181 ACCCTCCGGA TCCAAGCATG CCTTTAGCCC TATAACGAGC TAATGTTAGC TCCATCTTTG
  241 CACTTATGAT TGGATCAGCC CTCAAACGCT TTTGTATCTT TGCAGCTTCC GCGAAGGTAG
  301 TAGCTTGAAG TTTTTCATCC ATAGTTCTTG CTAAAATTGC AGAATCTTCA AACAATTCTA
  361 TGGCCTCGTC AATTTTTTCC ATTTGTAATT TTAGTTGTGC TAACCCGATC TTCGCTTGTT
  421 CAGATCGTGG ATCTAATTCA CAGGCTTTTG TCAGCAGCTT AATGGCTGCG TTGAATTTCT
  481 CTTCATCCAA CTGGGTTGGA TCTTGAGAGG ATTGTCTAGC TAATATAGTT GCTTTCCCAA
  541 TCAAGGGTCC TATACCCACA TGTATTTTTT CTTGCACTTC TTCCAACCTT TTAGCAATAT
  601 CATATTGTTT AATTGCAGTA TCAAAATCGC CCCTATCTGT CAAGATTTCA GCGAAAAAGG
  661 TGGGCACCTC AGGTAAGGTA GGAAACTTCA ACTTTGTTTC ATTGAAAAAA GCTTCAGATT
  721 CGGTAAATTT ACCTTGCTTG TACAACAAAC AGGCTAATTG AATGTAGGGA TAAACATTTT
  781 CAGGGTTCAA GCTTTGGGCC TTTTGGAAAT CTTCCTTGGC ATTTTTATAA TCTTGTAGAA
  841 TAAAATACAT CTGCCCACGG TGGTAATAGG TTGGCGGATA CTCAGGGTTT AGATCAACAG
  901 CTTTTTTGGA AAAAATTTGA AAAAATTCTT GAGAGTTTTC TTTATCGGCC CAAGGTCAAT
  961 GCCCAAAAAT ATGTACGAAT TTGGGTGTTT GGATGCAGGT TTTATAGATT TCTTGTTAAA
 1021 GACTTGGGGC AATCCTACAG CTTATTTTTT AGAATGGAGA TTACCCGTGT AGCACATTGC
1081 TCAGGGGCCA
//
Primer: TRP1pro_F (Pr0171)
Region: TRP1 pro, TRP1, ori
Sequence file: RDB16038_A8I3n.seq check
>D05803_A2_A8I3_1_TRP1pro_F_G01_19_ABI24.ab1
    1 GACTGGGATT AGCAACAAGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA CAGGTAGTTC
   61 TGGTCCATTG GTGAAAGTTT GCGGCTTGCA GAGCACAGAG GCCGCAGAAT GTGCTCTAGA
  121 TTCCGATGCT GACTTGCTGG GTATTATATG TGTGCCCAAT AGAAAGAGAA CAATTGACCC
  181 GGTTATTGCA AGGAAAATTT CAAGTCTTGT AAAAGCATAT AAAAATAGTT CAGGCACTCC
  241 GAAATACTTG GTTGGCGTGT TTCGTAATCA ACCTAAGGAG GATGTTTTGG CTCTGGTCAA
  301 TGATTACGGC ATTGATATCG TCCAACTGCA TGGAGATGAG TCGTGGCAAG AATACCAAGA
  361 GTTCCTCGGT TTGCCAGTTA TTAAAAGACT CGTATTTCCA AAAGACTGCA ACATACTACT
  421 CAGTGCAGCT TCACAGAAAC CTCATTCGTT TATTCCCTTG TTTGATTCAG AAGCAGGTGG
  481 GACAGGTGAA CTTTTGGATT GGAACTCGAT TTCTGACTGG GTTGGAAGGC AAGAGAGCCC
  541 CGAAAGCTTA CATTTTATGT TAGCTGGTGG ACTGACGCCA GAAAATGTTG GTGATGCGCT
  601 TAGATTAAAT GGCGTTATTG GTGTTGATGT AAGCGGAGGT GTGGAGACAA ATGGTGTAAA
  661 AGACTCTAAC AAAATAGCAA ATTTCGTCAA AAATGCTAAG AAATAGGTTA TTACTGAGTA
  721 GTATTTATTT AAGTATTGTT TGTGCACTTG CCGATCTATG CGGTGTGAAA TACCGCACAG
  781 ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAGCG TTAATATTTT GTTAAAATTC
  841 GCGTTAAATT TTTTGTTAAA TCAGCTCATT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
  901 TCCCTTTATA AATCAAAAGA AATAGACCGA GATAGGGTTT GAGTGTTTGT TTCCAGTTTG
  961 GAACAAGAGT CCACTATTTA AAGAACGGTG GTACCTCCAA CGGTCCAAAG GGCGAAAAAA
 1021 GTCGTCCTAT CATGATCGAT GGACCCAACT TACGTTGATC AATTCACCCT TATCAAGTTT
1081 TTTGGGGTTC GAGTGCCGGT AAAAG
//
Primer: TDH3pro_F (Pr0431)
Region: insert mid (GPD pro, TOM71 5')
Sequence file: RDB16038_A8I3o.seq check
>16038_16038_B0JcF_TDH3pro_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNT GNNNNTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA
   61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
  121 GCCGCATGGC CGAAAACTCC CTCCTGAGGT TTATCACCAA GAACAAAGTG GCCATCCTAG
  181 CAACGGTCTC TGCGGGGACT GCTGCTGTGG GTGCTTATGT TTACTACCAG CAAATTAAGC
  241 AGCAGCAACA GCAGCAATTG AAGGGTACAA AAGACAACCG ACGTCAAAGT GAGGCCTTTG
  301 CTGGTCAAAA TGAAGACGAG GCGGACTTAA AGGACGACGG CAGCGTCGTA AGTGGAAGTA
  361 ATAAGAGGAA GAAGAAGAAG AATAAACGCA AGAGGAACAA TAAGGCAAAA TCGGGAGAAG
  421 GGTTTGACTA TCCCTCCTTG CCCAATGGCG AGCCTGATAT TGCCCAATTG AAAGGCCTAT
  481 CACCCTCCCA GAGACAAGCG TATGCCGTGC AACTGAAGAA CAGAGGTAAC CACTTCTTCA
  541 CTGCTAAGAA TTTTAATGAG GCCATCAAAT ACTACCAGTA TGCCATTGAG CTGGANCCGA
  601 ATGAACCAGT GTTCTATTCC AACATATCTG CTTGTTACAT TTCTACNGGC GANTTANAGA
  661 ANGNTATAGA GNNCACTACA AANGNACTTG ANANNAAANC NGNNNNTTCN AAAGNCNTTG
721 CNNNNNNNNN N
//
Primer: Amp_R (Pr0008)
Region: AmpR pro, CEN_ARS
Sequence file: RDB16038_A8I3p.seq check
>D05803_A2_A8I3_1_Amp_R_H01_22_ABI24.ab1
    1 TTCTTTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
  781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GCGCGTCAGC GGTGTTGCGG TGTCGGGGCT
  841 GGCTTACTAT GCGGCATCAG AGCAGATTGT ACTGGAGAGT GCCACCATAG ATCACGACTT
  901 ACTATATATT ATAATATACG AGCATTATAG AACAGGCATC GTAATATATG TGTACTTGCA
  961 GGTTATGACG CGATGCAGTA ATGAAGATAT TCCTTAATGG AAAATAGCCT GGTCACCTTA
1021 CGTACAATCT TGAATCGCGA CTTACGTATA TGCCGATAGA ATCATTCGTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]