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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Tom71-GFP11

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Catalog number RDB16038
Resource name Tom71-GFP11
Alternative name pRS314-GPDp-Tom71-GFP11 (pSFL23)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae TOM71 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae TOM71

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16038 Tom71-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16038_A8I3p1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: insert 5' (T7 pro, GPD pro)
          Sequence file: RDB16038_A8I3k.seq check
          >16038_16038_B0JcF_HB-NOS_R_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTTGGGTNNN GCCNGGGTTT TCCCNGTCAC GACGTTGTAA
             61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC
            121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA
            181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA
            241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG
            301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC
            361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG
            421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT
            481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA
            541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG
            601 GNTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGANGGTA
            661 NGNANTGATT GNAATTCTGT AAATCTATTT CNTAAACTTC NTNANTTCTA CTTTTATAGT
          721 TAGNCTTTTT TTTNNNNTTT NAAANNNNNN NNANNNNNNN NNN
          //
          Primer: SV40pA3'Element Reverse
          Region: insert 3' (T3 pro, CYC1 ter, GFP11)
          Sequence file: RDB16038_A8I3l.seq check
          >16038_16038_A9LbF_SV40pA3'elementReverse_A03_03_ABI24.ab1
              1 NNNNNNNNNG GNNNNNNNNA NGGNNAANGC NNGCAACGCG GCCTTTTTAC GGTTCCTGGC
             61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
            121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
            181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
            241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
            301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
            361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
            421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
            481 ACCGGCCGCA AATTAAAGCC TTCGAGCGTC CCAAAACCTT CTCAAGCAAG GTTTTCAGTA
            541 TAATGTTACA TGCGTACACG CGTCTGTACA GAAAAAAAAG AAAAATTTGA AATATAAATA
            601 ACGTTCTTAA TACTAACATA ACTATAAAAA AATAAATAGG GACCTAGACT TCAGGTTGTC
            661 TAACTCCTTC CTTTTCGGTT AGAGCGGATG TGGGGGGAGG GCGTGAATGT AAGCGTGACA
            721 TAACTAATTA CATGACTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCTTATGT
            781 AATCCCAGCA GCATTTACGT ACTCATGAAG GANCATGTGG TCACGANTTG TTGATCCGCC
            841 NCCAGANCCT CCGGATNCAA GCATGCCTTT AGCCCNATAA CGANCNAANG TNANCTNCNT
            901 CTTTGCACTN ATGATNNGAT CAGCCCNCAA ACGCTTTTGT ANNNTNNCNG CTNCNNNGAA
          961 NNNTANNANC NNGNANNTTT TNCNTCCNNA NNNNTNGNNN AANNTNNNNN NNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP11, TOM71 3')
          Sequence file: RDB16038_A8I3m.seq check
          >D05803_A2_A8I3_1_CYC1ter_R_B01_04_ABI24.ab1
              1 CTTTCGTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
             61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TATGTAATCC
            121 CAGCAGCATT TACGTACTCA TGAAGGACCA TGTGGTCACG ACTTGTTGAT CCGCCACCAG
            181 ACCCTCCGGA TCCAAGCATG CCTTTAGCCC TATAACGAGC TAATGTTAGC TCCATCTTTG
            241 CACTTATGAT TGGATCAGCC CTCAAACGCT TTTGTATCTT TGCAGCTTCC GCGAAGGTAG
            301 TAGCTTGAAG TTTTTCATCC ATAGTTCTTG CTAAAATTGC AGAATCTTCA AACAATTCTA
            361 TGGCCTCGTC AATTTTTTCC ATTTGTAATT TTAGTTGTGC TAACCCGATC TTCGCTTGTT
            421 CAGATCGTGG ATCTAATTCA CAGGCTTTTG TCAGCAGCTT AATGGCTGCG TTGAATTTCT
            481 CTTCATCCAA CTGGGTTGGA TCTTGAGAGG ATTGTCTAGC TAATATAGTT GCTTTCCCAA
            541 TCAAGGGTCC TATACCCACA TGTATTTTTT CTTGCACTTC TTCCAACCTT TTAGCAATAT
            601 CATATTGTTT AATTGCAGTA TCAAAATCGC CCCTATCTGT CAAGATTTCA GCGAAAAAGG
            661 TGGGCACCTC AGGTAAGGTA GGAAACTTCA ACTTTGTTTC ATTGAAAAAA GCTTCAGATT
            721 CGGTAAATTT ACCTTGCTTG TACAACAAAC AGGCTAATTG AATGTAGGGA TAAACATTTT
            781 CAGGGTTCAA GCTTTGGGCC TTTTGGAAAT CTTCCTTGGC ATTTTTATAA TCTTGTAGAA
            841 TAAAATACAT CTGCCCACGG TGGTAATAGG TTGGCGGATA CTCAGGGTTT AGATCAACAG
            901 CTTTTTTGGA AAAAATTTGA AAAAATTCTT GAGAGTTTTC TTTATCGGCC CAAGGTCAAT
            961 GCCCAAAAAT ATGTACGAAT TTGGGTGTTT GGATGCAGGT TTTATAGATT TCTTGTTAAA
           1021 GACTTGGGGC AATCCTACAG CTTATTTTTT AGAATGGAGA TTACCCGTGT AGCACATTGC
          1081 TCAGGGGCCA
          //
          Primer: TRP1pro_F
          Region: TRP1 pro, TRP1, ori
          Sequence file: RDB16038_A8I3n.seq check
          >D05803_A2_A8I3_1_TRP1pro_F_G01_19_ABI24.ab1
              1 GACTGGGATT AGCAACAAGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA CAGGTAGTTC
             61 TGGTCCATTG GTGAAAGTTT GCGGCTTGCA GAGCACAGAG GCCGCAGAAT GTGCTCTAGA
            121 TTCCGATGCT GACTTGCTGG GTATTATATG TGTGCCCAAT AGAAAGAGAA CAATTGACCC
            181 GGTTATTGCA AGGAAAATTT CAAGTCTTGT AAAAGCATAT AAAAATAGTT CAGGCACTCC
            241 GAAATACTTG GTTGGCGTGT TTCGTAATCA ACCTAAGGAG GATGTTTTGG CTCTGGTCAA
            301 TGATTACGGC ATTGATATCG TCCAACTGCA TGGAGATGAG TCGTGGCAAG AATACCAAGA
            361 GTTCCTCGGT TTGCCAGTTA TTAAAAGACT CGTATTTCCA AAAGACTGCA ACATACTACT
            421 CAGTGCAGCT TCACAGAAAC CTCATTCGTT TATTCCCTTG TTTGATTCAG AAGCAGGTGG
            481 GACAGGTGAA CTTTTGGATT GGAACTCGAT TTCTGACTGG GTTGGAAGGC AAGAGAGCCC
            541 CGAAAGCTTA CATTTTATGT TAGCTGGTGG ACTGACGCCA GAAAATGTTG GTGATGCGCT
            601 TAGATTAAAT GGCGTTATTG GTGTTGATGT AAGCGGAGGT GTGGAGACAA ATGGTGTAAA
            661 AGACTCTAAC AAAATAGCAA ATTTCGTCAA AAATGCTAAG AAATAGGTTA TTACTGAGTA
            721 GTATTTATTT AAGTATTGTT TGTGCACTTG CCGATCTATG CGGTGTGAAA TACCGCACAG
            781 ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAGCG TTAATATTTT GTTAAAATTC
            841 GCGTTAAATT TTTTGTTAAA TCAGCTCATT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
            901 TCCCTTTATA AATCAAAAGA AATAGACCGA GATAGGGTTT GAGTGTTTGT TTCCAGTTTG
            961 GAACAAGAGT CCACTATTTA AAGAACGGTG GTACCTCCAA CGGTCCAAAG GGCGAAAAAA
           1021 GTCGTCCTAT CATGATCGAT GGACCCAACT TACGTTGATC AATTCACCCT TATCAAGTTT
          1081 TTTGGGGTTC GAGTGCCGGT AAAAG
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, TOM71 5')
          Sequence file: RDB16038_A8I3o.seq check
          >16038_16038_B0JcF_TDH3pro_F_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNTGNNT GNNNNTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA
             61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
            121 GCCGCATGGC CGAAAACTCC CTCCTGAGGT TTATCACCAA GAACAAAGTG GCCATCCTAG
            181 CAACGGTCTC TGCGGGGACT GCTGCTGTGG GTGCTTATGT TTACTACCAG CAAATTAAGC
            241 AGCAGCAACA GCAGCAATTG AAGGGTACAA AAGACAACCG ACGTCAAAGT GAGGCCTTTG
            301 CTGGTCAAAA TGAAGACGAG GCGGACTTAA AGGACGACGG CAGCGTCGTA AGTGGAAGTA
            361 ATAAGAGGAA GAAGAAGAAG AATAAACGCA AGAGGAACAA TAAGGCAAAA TCGGGAGAAG
            421 GGTTTGACTA TCCCTCCTTG CCCAATGGCG AGCCTGATAT TGCCCAATTG AAAGGCCTAT
            481 CACCCTCCCA GAGACAAGCG TATGCCGTGC AACTGAAGAA CAGAGGTAAC CACTTCTTCA
            541 CTGCTAAGAA TTTTAATGAG GCCATCAAAT ACTACCAGTA TGCCATTGAG CTGGANCCGA
            601 ATGAACCAGT GTTCTATTCC AACATATCTG CTTGTTACAT TTCTACNGGC GANTTANAGA
            661 ANGNTATAGA GNNCACTACA AANGNACTTG ANANNAAANC NGNNNNTTCN AAAGNCNTTG
          721 CNNNNNNNNN N
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB16038_A8I3p.seq check
          >D05803_A2_A8I3_1_Amp_R_H01_22_ABI24.ab1
              1 TTCTTTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
             61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
            121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
            181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
            241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
            301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
            361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
            421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
            481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
            541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
            601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
            661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
            721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
            781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GCGCGTCAGC GGTGTTGCGG TGTCGGGGCT
            841 GGCTTACTAT GCGGCATCAG AGCAGATTGT ACTGGAGAGT GCCACCATAG ATCACGACTT
            901 ACTATATATT ATAATATACG AGCATTATAG AACAGGCATC GTAATATATG TGTACTTGCA
            961 GGTTATGACG CGATGCAGTA ATGAAGATAT TCCTTAATGG AAAATAGCCT GGTCACCTTA
          1021 CGTACAATCT TGAATCGCGA CTTACGTATA TGCCGATAGA ATCATTCGTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl