Please review the QC test results indicated by icon below as well as clone information before placing your order.

Tom71-GFP11

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP11; location: mitochondria.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16038_A8I3p1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: insert 5' (T7 pro, GPD pro) Sequence file: RDB16038_A8I3k.seq
>16038_16038_B0JcF_HB-NOS_R_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTGGGTNNN GCCNGGGTTT TCCCNGTCAC GACGTTGTAA    61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC   121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA   181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA   241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG   301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC   361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG   421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT   481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA   541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG   601 GNTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGANGGTA   661 NGNANTGATT GNAATTCTGT AAATCTATTT CNTAAACTTC NTNANTTCTA CTTTTATAGT 721 TAGNCTTTTT TTTNNNNTTT NAAANNNNNN NNANNNNNNN NNN //
Primer: SV40pA3'Element Reverse (Pr0108) Region: insert 3' (T3 pro, CYC1 ter, GFP11) Sequence file: RDB16038_A8I3l.seq
>16038_16038_A9LbF_SV40pA3'elementReverse_A03_03_ABI24.ab1     1 NNNNNNNNNG GNNNNNNNNA NGGNNAANGC NNGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA   121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG   181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG   241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA   301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT   361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG   421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT   481 ACCGGCCGCA AATTAAAGCC TTCGAGCGTC CCAAAACCTT CTCAAGCAAG GTTTTCAGTA   541 TAATGTTACA TGCGTACACG CGTCTGTACA GAAAAAAAAG AAAAATTTGA AATATAAATA   601 ACGTTCTTAA TACTAACATA ACTATAAAAA AATAAATAGG GACCTAGACT TCAGGTTGTC   661 TAACTCCTTC CTTTTCGGTT AGAGCGGATG TGGGGGGAGG GCGTGAATGT AAGCGTGACA   721 TAACTAATTA CATGACTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCTTATGT   781 AATCCCAGCA GCATTTACGT ACTCATGAAG GANCATGTGG TCACGANTTG TTGATCCGCC   841 NCCAGANCCT CCGGATNCAA GCATGCCTTT AGCCCNATAA CGANCNAANG TNANCTNCNT   901 CTTTGCACTN ATGATNNGAT CAGCCCNCAA ACGCTTTTGT ANNNTNNCNG CTNCNNNGAA 961 NNNTANNANC NNGNANNTTT TNCNTCCNNA NNNNTNGNNN AANNTNNNNN NNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP11, TOM71 3') Sequence file: RDB16038_A8I3m.seq
>D05803_A2_A8I3_1_CYC1ter_R_B01_04_ABI24.ab1     1 CTTTCGTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT    61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TATGTAATCC   121 CAGCAGCATT TACGTACTCA TGAAGGACCA TGTGGTCACG ACTTGTTGAT CCGCCACCAG   181 ACCCTCCGGA TCCAAGCATG CCTTTAGCCC TATAACGAGC TAATGTTAGC TCCATCTTTG   241 CACTTATGAT TGGATCAGCC CTCAAACGCT TTTGTATCTT TGCAGCTTCC GCGAAGGTAG   301 TAGCTTGAAG TTTTTCATCC ATAGTTCTTG CTAAAATTGC AGAATCTTCA AACAATTCTA   361 TGGCCTCGTC AATTTTTTCC ATTTGTAATT TTAGTTGTGC TAACCCGATC TTCGCTTGTT   421 CAGATCGTGG ATCTAATTCA CAGGCTTTTG TCAGCAGCTT AATGGCTGCG TTGAATTTCT   481 CTTCATCCAA CTGGGTTGGA TCTTGAGAGG ATTGTCTAGC TAATATAGTT GCTTTCCCAA   541 TCAAGGGTCC TATACCCACA TGTATTTTTT CTTGCACTTC TTCCAACCTT TTAGCAATAT   601 CATATTGTTT AATTGCAGTA TCAAAATCGC CCCTATCTGT CAAGATTTCA GCGAAAAAGG   661 TGGGCACCTC AGGTAAGGTA GGAAACTTCA ACTTTGTTTC ATTGAAAAAA GCTTCAGATT   721 CGGTAAATTT ACCTTGCTTG TACAACAAAC AGGCTAATTG AATGTAGGGA TAAACATTTT   781 CAGGGTTCAA GCTTTGGGCC TTTTGGAAAT CTTCCTTGGC ATTTTTATAA TCTTGTAGAA   841 TAAAATACAT CTGCCCACGG TGGTAATAGG TTGGCGGATA CTCAGGGTTT AGATCAACAG   901 CTTTTTTGGA AAAAATTTGA AAAAATTCTT GAGAGTTTTC TTTATCGGCC CAAGGTCAAT   961 GCCCAAAAAT ATGTACGAAT TTGGGTGTTT GGATGCAGGT TTTATAGATT TCTTGTTAAA  1021 GACTTGGGGC AATCCTACAG CTTATTTTTT AGAATGGAGA TTACCCGTGT AGCACATTGC 1081 TCAGGGGCCA //
Primer: TRP1pro_F (Pr0171) Region: TRP1 pro, TRP1, ori Sequence file: RDB16038_A8I3n.seq
>D05803_A2_A8I3_1_TRP1pro_F_G01_19_ABI24.ab1     1 GACTGGGATT AGCAACAAGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA CAGGTAGTTC    61 TGGTCCATTG GTGAAAGTTT GCGGCTTGCA GAGCACAGAG GCCGCAGAAT GTGCTCTAGA   121 TTCCGATGCT GACTTGCTGG GTATTATATG TGTGCCCAAT AGAAAGAGAA CAATTGACCC   181 GGTTATTGCA AGGAAAATTT CAAGTCTTGT AAAAGCATAT AAAAATAGTT CAGGCACTCC   241 GAAATACTTG GTTGGCGTGT TTCGTAATCA ACCTAAGGAG GATGTTTTGG CTCTGGTCAA   301 TGATTACGGC ATTGATATCG TCCAACTGCA TGGAGATGAG TCGTGGCAAG AATACCAAGA   361 GTTCCTCGGT TTGCCAGTTA TTAAAAGACT CGTATTTCCA AAAGACTGCA ACATACTACT   421 CAGTGCAGCT TCACAGAAAC CTCATTCGTT TATTCCCTTG TTTGATTCAG AAGCAGGTGG   481 GACAGGTGAA CTTTTGGATT GGAACTCGAT TTCTGACTGG GTTGGAAGGC AAGAGAGCCC   541 CGAAAGCTTA CATTTTATGT TAGCTGGTGG ACTGACGCCA GAAAATGTTG GTGATGCGCT   601 TAGATTAAAT GGCGTTATTG GTGTTGATGT AAGCGGAGGT GTGGAGACAA ATGGTGTAAA   661 AGACTCTAAC AAAATAGCAA ATTTCGTCAA AAATGCTAAG AAATAGGTTA TTACTGAGTA   721 GTATTTATTT AAGTATTGTT TGTGCACTTG CCGATCTATG CGGTGTGAAA TACCGCACAG   781 ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAGCG TTAATATTTT GTTAAAATTC   841 GCGTTAAATT TTTTGTTAAA TCAGCTCATT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA   901 TCCCTTTATA AATCAAAAGA AATAGACCGA GATAGGGTTT GAGTGTTTGT TTCCAGTTTG   961 GAACAAGAGT CCACTATTTA AAGAACGGTG GTACCTCCAA CGGTCCAAAG GGCGAAAAAA  1021 GTCGTCCTAT CATGATCGAT GGACCCAACT TACGTTGATC AATTCACCCT TATCAAGTTT 1081 TTTGGGGTTC GAGTGCCGGT AAAAG //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, TOM71 5') Sequence file: RDB16038_A8I3o.seq
>16038_16038_B0JcF_TDH3pro_F_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNTGNNT GNNNNTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA    61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG   121 GCCGCATGGC CGAAAACTCC CTCCTGAGGT TTATCACCAA GAACAAAGTG GCCATCCTAG   181 CAACGGTCTC TGCGGGGACT GCTGCTGTGG GTGCTTATGT TTACTACCAG CAAATTAAGC   241 AGCAGCAACA GCAGCAATTG AAGGGTACAA AAGACAACCG ACGTCAAAGT GAGGCCTTTG   301 CTGGTCAAAA TGAAGACGAG GCGGACTTAA AGGACGACGG CAGCGTCGTA AGTGGAAGTA   361 ATAAGAGGAA GAAGAAGAAG AATAAACGCA AGAGGAACAA TAAGGCAAAA TCGGGAGAAG   421 GGTTTGACTA TCCCTCCTTG CCCAATGGCG AGCCTGATAT TGCCCAATTG AAAGGCCTAT   481 CACCCTCCCA GAGACAAGCG TATGCCGTGC AACTGAAGAA CAGAGGTAAC CACTTCTTCA   541 CTGCTAAGAA TTTTAATGAG GCCATCAAAT ACTACCAGTA TGCCATTGAG CTGGANCCGA   601 ATGAACCAGT GTTCTATTCC AACATATCTG CTTGTTACAT TTCTACNGGC GANTTANAGA   661 ANGNTATAGA GNNCACTACA AANGNACTTG ANANNAAANC NGNNNNTTCN AAAGNCNTTG 721 CNNNNNNNNN N //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB16038_A8I3p.seq
>D05803_A2_A8I3_1_Amp_R_H01_22_ABI24.ab1     1 TTCTTTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA   181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC   241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC   301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA   361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT   421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT   481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC   541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA   601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC   661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT   721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA   781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GCGCGTCAGC GGTGTTGCGG TGTCGGGGCT   841 GGCTTACTAT GCGGCATCAG AGCAGATTGT ACTGGAGAGT GCCACCATAG ATCACGACTT   901 ACTATATATT ATAATATACG AGCATTATAG AACAGGCATC GTAATATATG TGTACTTGCA   961 GGTTATGACG CGATGCAGTA ATGAAGATAT TCCTTAATGG AAAATAGCCT GGTCACCTTA 1021 CGTACAATCT TGAATCGCGA CTTACGTATA TGCCGATAGA ATCATTCGTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl