Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.
Catalog number | RDB16037 |
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Resource name | Tom71-GFP1-10 |
Alternative name | pRS316-GPDp-Tom71-GFP1-10 (pSFL22) |
Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS316 (plasmid) |
Selectable markers | Amp^r (E. coli), URA3 |
Gene/insert name | S. cerevisiae TOM71 cDNA |
Depositor|Developer | Tamura, Yasushi | |
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Other clones in our bank | S. cerevisiae TOM71 | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Schedule A (GFP-A) [Link] |
Remarks | Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16037 | Tom71-GFP1-10 | DNA solution |
Electronic file
Original reference
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Dnaconda's recommendation EXR0090j (Japanese text) |
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Featured content | Dnaconda's recommendation EXR0090e (English text) |
Featured content | Visualization of Organelles (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16037_A8I3p1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: HB-NOS_R Region: insert 5' (T7 pro, GPD pro) Sequence file: RDB16037_A8I3i.seq ![]() |
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>16037_16037_B0IuF_HB-Nos_R_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNGNNNNNN NTGGGTNNNG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA 61 ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CAGTTTATCA 121 TTATCAATAC TCGCCATTTC AAAGAATACG TAAATAATTA ATAGTAGTGA TTTTCCTAAC 181 TTTATTTAGT CAAAAAATTA GCCTTTTAAT TCTGCTGTAA CCCGTACATG CCCAAAATAG 241 GGGGCGGGTT ACACAGAATA TATAACATCG TAGGTGTCTG GGTGAACAGT TTATTCCTGG 301 CATCCACTAA ATATAATGGA GCCCGCTTTT TAAGCTGGCA TCCAGAAAAA AAAAGAATCC 361 CAGCACCAAA ATATTGTTTT CTTCACCAAC CATCAGTTCA TAGGTCCATT CTCTTAGCGC 421 AACTACAGAG AACAGGGGCA CAAACAGGCA AAAAACGGGC ACAACCTCAA TGGAGTGATG 481 CAACCTGCCT GGAGTAAATG ATGACACAAG GCAATTGACC CACGCATGTA TCTATCTCAT 541 TTTCTTACAC CTTCTATTAC CTTCTGCTCT CTCTGATTTG GAAAAAGCTG AAAAAAAAGG 601 NTGAAACCAG TTCCCTGAAA TTATNNNNTA CTTGACTAAT AAGTATATAA AGACNGNANG 661 TANTGANTGT AANTCTGNAN NCNATTTCNN NAACTTNNTN AANNCNNNNT TNNNNNNNNN 721 N // |
Primer: SV40pA3'Element Reverse Region: insert 3' (T3 pro, CYC1 ter, GFP1-10 3') Sequence file: RDB16037_A8I3j.seq ![]() |
>16037_16037_B0IuF_SV40pA3'elementReverse_G05_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNCNNGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 61 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 121 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 181 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 241 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 301 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 361 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 421 ACCATGATTA CGCCAAGCTC GAAATTAACC CTCACTAAAG GGAACAAAAG CTGGTACCGG 481 CCGCAAATTA AAGCCTTCGA GCGTCCCAAA ACCTTCTCAA GCAAGGTTTT CAGTATAATG 541 TTACATGCGT ACACGCGTCT GTACAGAAAA AAAAGAAAAA TTTGAAATAT AAATAACGTT 601 CTTAATACTA ACATAACTAT AAAAAAATAA ATAGGGACCT AGACTTCAGG TTGTCTAACT 661 CCTTCCTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT 721 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT 781 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG 841 GGCCATCGCC NATNNGANTA NTTTGTTGAT AATGGNCTGC NNNTTGAACG GAANCNTCTT 901 CNNCGTTGNG GNGAANNGTN AAGTTAGCTT NNANTNCNTT CNTTTGNTNG NCNGNCNNGA 961 NGNNNACNTN GNGNNANNNN ANNNNGNNNN NNNNNTNNNN NNNNNN // |
Primer: paGFP-M_R1 Region: insert mid (GFP1-10 5', TOM71 3') Sequence file: RDB16037_A8I3k.seq ![]() |
>16037_16037_B0IuF_paGFP-M_R1_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNTGCCTT TTNNTGTGAT CCGGATAACG GGAAAAGCAT TGAACACCAT 61 AGGTCAGAGT AGTGACAAGT GTTGGCCATG GAACAGGTAG TTTTCCAGTA GTGCAAATAA 121 ATTTAAGTGT GAGTTTTCCG ATTGTAGCAT CACCTTCACC CTCTCCTCTG ACAGAAAATT 181 TGTGCCCATT AACATCACCA TCTAATTCAA CAAGAATTGG GACAACTCCA GTGAAAAGTT 241 CTTCTCCTTT GCTCATACTA GTGCCACCCA TTGATCCGCC ACCAGACCCT CCGGATCCAA 301 GCATGCCTTT AGCCCTATAA CGAGCTAATG TTAGCTCCAT CTTTGCACTT ATGATTGGAT 361 CAGCCCTCAA ACGCTTTTGT ATCTTTGCAG CTTCCGCGAA GGTAGTAGCT TGAAGTTTTT 421 CATCCATAGT TCTTGCTAAA ATTGCAGAAT CTTCAAACAA TTCTATGGCC TCGTCAATTT 481 TTTCCATTTG TAATTTTAGT TGTGCTAACC CGATCTTCGC TTGTTCAGAT CGTGGATCTA 541 ATTCACAGGC TTTTGTCAGC AGCTTAATGG CTGCGTTGAA TTTCTCTTCN TCCAACTGGG 601 TTGGATCTTG AGAGGANTGT CTAGCTAATA TAGTTGCTTT CCCNATCAAG GGTCCTATAN 661 CCACATGTAT TTTTTCNNGN ACTTCTTCCN ANCTTTNANN NNTANCATAN NNNTNNANTN 721 NNNNNNNNN // |
Primer: URA3pro_F Region: URA3pro, URA3 Sequence file: RDB16037_A8I3l.seq ![]() |
>D05803_A1_A8I3_1_URA3pro_F_C01_07_ABI24.ab1 1 CAAATGTAGT GTTGAGAACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA 61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC 121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT 181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG 241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG 301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG 361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT 421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG 481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA 541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC 601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA 661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC 721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT 781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG 841 AAAAGCAGGC TGGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA 901 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG 961 TTTATTACCC TATGCGGTGT GAATACCGCA CAGATGCGTA AAGGAGAAAA ATACCCGCAT 1021 TCAGAATTGT AAGCGGTATA TTTTGTTAAA ATTCGCGTTA ATTTTTGGTA AATTCAGCTC 1081 AATTTTTTTA AGTCA // |
Primer: TDH3pro_F Region: insert mid (GPD pro, TOM71 5') Sequence file: RDB16037_A8I3m.seq ![]() |
>16037_16037_B0IuF_TDH3pro_F_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNGATTG NAATTCTGTA AATCTATTTC TTAAACTTCT TAAATTCTAC 61 TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATGCGG 121 CCGCATGGCC GAAAACTCCC TCCTGAGGTT TATCACCAAG AACAAAGTGG CCATCCTAGC 181 AACGGTCTCT GCGGGGACTG CTGCTGTGGG TGCTTATGTT TACTACCAGC AAATTAAGCA 241 GCAGCAACAG CAGCAATTGA AGGGTACAAA AGACAACCGA CGTCAAAGTG AGGCCTTTGC 301 TGGTCAAAAT GAAGACGAGG CGGACTTAAA GGACGACGGC AGCGTCGTAA GTGGAAGTAA 361 TAAGAGGAAG AAGAAGAAGA ATAAACGCAA GAGGAACAAT AAGGCAAAAT CGGGAGAAGG 421 GTTTGACTAT CCCTCCTTGC CCAATGGCGA GCCTGATATT GCCCAATTGA AAGGCCTATC 481 ACCCTCCCAG AGACAAGCGT ATGCCGTGCA ACTGAAGAAC AGAGGTAACC ACTTCTTCAC 541 TGCTAAGAAT TTTAATGAGG CCATCAAATA CTACCAGTAT GCCATTGAGC TGGANCCGAA 601 TGANCCAGTG TTCTATTCCA ACATATCTGC TTGTTACATT TCTACNGGCG ANTTANAGAA 661 NNNTATAGAG NTCACTACAA ANGNNNNTGA NANAAAACCT GANNNTTCNA ANNNNNGNNN 721 ANNNNNNNNN N // |
Primer: CYC1ter_R Region: insert mid (CYC1 ter, GFP1-10 3') Sequence file: RDB16037_A8I3n.seq ![]() |
>D05803_A1_A8I3_1_CYC1ter_R_A01_01_ABI24.ab1 1 ATTTCTTTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT GACATAACTA 61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ACTTTTCGTT 121 GGGATCTTTC GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG 181 GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGG AACCATCTTC 241 AACGTTGTGG CGAACTGTGA AGTTAGCTTT GATTCCATTC TTTTGTTTGT CTGCCGTGAT 301 GTATACATTG TGTGAGTTAA AGTTGTACTC GAGTTTGTGT CCGAGAATGT TTCCATCTTC 361 TTTAAAATCA GTACCCTTTA ACTCGATACG ATTAACAAGG GTATCACCTT CAAACTTGAC 421 TACAGCACGC GTCTTGTATT TCCCGTCATC TTTGAAAGAT ATAGTGCGTT CCTGTACATA 481 ACCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCCTTTTC ATGTGATCCG GATAACGGGA 541 AAAGCATTGA ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT 601 TCCAGTAGTG CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC 661 TCCTCTGACA GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC 721 AACTCCAGTG AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCCATTG ATCCGCCACC 781 AGACCCTCCG GATCCAAGCA TGCCTTTAGC CCTATAACGA GCTAATGTTA GCTCCATCTT 841 TGCACTTATG ATTGGATCAG CCCTCAAACG CTTTTTGTAT CTTTGCAGCT TCCGCGAAGG 901 TAGTAGCTTG AAGTTTTTTC ATCCATAGTT CTTGCTAAAA TTGCAGAATC TTCAAACAAA 961 TTCTATGGCC CTCGTCAATT TTTTTTCCCA TTTGTAAATT TTAGTTTGTG CCTACCCGAT 1021 CTTTCGCTTG TTTCAGATCG TTGGATCCTA AATTTCACAG GCTCTGTCAG CAGCTTGATG 1081 ACCTGCGATT GCAT // |
Primer: Amp_R Region: AmpR pro, CEN_ARS, URA3 pro Sequence file: RDB16037_A8I3o.seq ![]() |
>D05803_A1_A8I3_1_Amp_R_D01_10_ABI24.ab1 1 TCTTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA 181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC 241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC 301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA 361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT 421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT 481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC 541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA 601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC 661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT 721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA 781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG 841 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TACCACCTTT 901 TCAATTCATC ATTTTTTTTT TTTTATTCTT TTTTTTTGAT TTCGGTTTCC CTTTGAAATT 961 TTTTTTTGAT TTCGGTAAAT CTCCCGAACA GAAGGGAAGG AACGAATGGT GGAGCACAGA 1021 CTTTAGATTT GGTATATAAT ACGCATATGT AGGTGCTGAG AAACATGAAA TGCCCAGTAT 1081 CGTAACCGAA CTGCACAGAA CCAAAAACCG TTGCAGGA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. |
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2020.11.06
GNP_filter3_RDBDEP_html_201104.pl