Please review the QC test results indicated by icon below as well as clone information before placing your order.

Tom71-GFP1-10

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering	Please visit Information of Request for Distribution.  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16037_A8I3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Region: GPD pro, insert 5' Sequence file: RDB16037_A8I3e.seq
>D05803_A1_A8I3_1_M13_-40_A10_01_ABI24.ab1     1 AACAAGGCAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT    61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA   121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG   181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT   241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA   301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG   361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC   421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC   481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC   541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT   601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAaATTCT ACTTTTATAG TTAGTCTTTT   661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGG CCGAAAACTC   721 CCTCCTGAGG TTTATCACCA AGAACAAAGT GGCCATCCTA GCAACGGTCT CTGCGGGGAC   781 TGCTGCTGTG GGTGCTTATG TTTACTACCA GCAAATTAAG CAGCAGCAAC AGCAGCAATT   841 GAAGGGTACA AAAGACAACC CGACGTCAAA GTGAGGCCTT TGCTGGGTCA AATGAAGACG   901 AGGCGGACTT AAAGGACGAC GGCAGCGTCG TAGTGAGTAT AGAGAGAGGA GAGATAACGC   961 AGAGACATAG CAAATCGGAG AACGTTGACT ATCCCTCCTG CCAATTGGCG AGCCTGGAAT 1021 TGGCCAGATG AAGCCTATAC TCCTCCTGAG AACAGCCGA //
Primer: CYC1ter_R Region: CYC1 ter, insert 3' Sequence file: RDB16037_A8I3f.seq
>D05803_A1_A8I3_1_CYC1ter_R_A01_01_ABI24.ab1     1 ATTTCTTTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT GACATAACTA    61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ACTTTTCGTT   121 GGGATCTTTC GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG   181 GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGG AACCATCTTC   241 AACGTTGTGG CGAACTGTGA AGTTAGCTTT GATTCCATTC TTTTGTTTGT CTGCCGTGAT   301 GTATACATTG TGTGAGTTAA AGTTGTACTC GAGTTTGTGT CCGAGAATGT TTCCATCTTC   361 TTTAAAATCA GTACCCTTTA ACTCGATACG ATTAACAAGG GTATCACCTT CAAACTTGAC   421 TACAGCACGC GTCTTGTATT TCCCGTCATC TTTGAAAGAT ATAGTGCGTT CCTGTACATA   481 ACCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCCTTTTC ATGTGATCCG GATAACGGGA   541 AAAGCATTGA ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT   601 TCCAGTAGTG CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC   661 TCCTCTGACA GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC   721 AACTCCAGTG AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCCATTG ATCCGCCACC   781 AGACCCTCCG GATCCAAGCA TGCCTTTAGC CCTATAACGA GCTAATGTTA GCTCCATCTT   841 TGCACTTATG ATTGGATCAG CCCTCAAACG CTTTTTGTAT CTTTGCAGCT TCCGCGAAGG   901 TAGTAGCTTG AAGTTTTTTC ATCCATAGTT CTTGCTAAAA TTGCAGAATC TTCAAACAAA   961 TTCTATGGCC CTCGTCAATT TTTTTTCCCA TTTGTAAATT TTAGTTTGTG CCTACCCGAT  1021 CTTTCGCTTG TTTCAGATCG TTGGATCCTA AATTTCACAG GCTCTGTCAG CAGCTTGATG 1081 ACCTGCGATT GCAT //
Primer: URA3pro_F Region: URA3 Sequence file: RDB16037_A8I3g.seq
>D05803_A1_A8I3_1_URA3pro_F_C01_07_ABI24.ab1     1 CAAATGTAGT GTTGAGAACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA    61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC   121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT   181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG   241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG   301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG   361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT   421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG   481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA   541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC   601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA   661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC   721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT   781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG   841 AAAAGCAGGC TGGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA   901 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG   961 TTTATTACCC TATGCGGTGT GAATACCGCA CAGATGCGTA AAGGAGAAAA ATACCCGCAT  1021 TCAGAATTGT AAGCGGTATA TTTTGTTAAA ATTCGCGTTA ATTTTTGGTA AATTCAGCTC 1081 AATTTTTTTA AGTCA //
Primer: Amp_R Region: CEN/ARS Sequence file: RDB16037_A8I3h.seq
>D05803_A1_A8I3_1_Amp_R_D01_10_ABI24.ab1     1 TCTTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA   181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC   241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC   301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA   361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT   421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT   481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC   541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA   601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC   661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT   721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA   781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG   841 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TACCACCTTT   901 TCAATTCATC ATTTTTTTTT TTTTATTCTT TTTTTTTGAT TTCGGTTTCC CTTTGAAATT   961 TTTTTTTGAT TTCGGTAAAT CTCCCGAACA GAAGGGAAGG AACGAATGGT GGAGCACAGA  1021 CTTTAGATTT GGTATATAAT ACGCATATGT AGGTGCTGAG AAACATGAAA TGCCCAGTAT 1081 CGTAACCGAA CTGCACAGAA CCAAAAACCG TTGCAGGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl