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Tom71-GFP1-10

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Catalog number RDB16037
Resource name Tom71-GFP1-10
Alternative name pRS316-GPDp-Tom71-GFP1-10 (pSFL22)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae TOM71 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae TOM71

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16037 Tom71-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB16037zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16037_A8I3p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: insert 5' (T7 pro, GPD pro)
          Sequence file: RDB16037_A8I3i.seq check
          >16037_16037_B0IuF_HB-Nos_R_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNGNNNNNN NTGGGTNNNG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA
             61 ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CAGTTTATCA
            121 TTATCAATAC TCGCCATTTC AAAGAATACG TAAATAATTA ATAGTAGTGA TTTTCCTAAC
            181 TTTATTTAGT CAAAAAATTA GCCTTTTAAT TCTGCTGTAA CCCGTACATG CCCAAAATAG
            241 GGGGCGGGTT ACACAGAATA TATAACATCG TAGGTGTCTG GGTGAACAGT TTATTCCTGG
            301 CATCCACTAA ATATAATGGA GCCCGCTTTT TAAGCTGGCA TCCAGAAAAA AAAAGAATCC
            361 CAGCACCAAA ATATTGTTTT CTTCACCAAC CATCAGTTCA TAGGTCCATT CTCTTAGCGC
            421 AACTACAGAG AACAGGGGCA CAAACAGGCA AAAAACGGGC ACAACCTCAA TGGAGTGATG
            481 CAACCTGCCT GGAGTAAATG ATGACACAAG GCAATTGACC CACGCATGTA TCTATCTCAT
            541 TTTCTTACAC CTTCTATTAC CTTCTGCTCT CTCTGATTTG GAAAAAGCTG AAAAAAAAGG
            601 NTGAAACCAG TTCCCTGAAA TTATNNNNTA CTTGACTAAT AAGTATATAA AGACNGNANG
            661 TANTGANTGT AANTCTGNAN NCNATTTCNN NAACTTNNTN AANNCNNNNT TNNNNNNNNN
          721 N
          //
          Primer: SV40pA3'Element Reverse
          Region: insert 3' (T3 pro, CYC1 ter, GFP1-10 3')
          Sequence file: RDB16037_A8I3j.seq check
          >16037_16037_B0IuF_SV40pA3'elementReverse_G05_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNCNNGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
             61 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
            121 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
            181 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
            241 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
            301 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
            361 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG
            421 ACCATGATTA CGCCAAGCTC GAAATTAACC CTCACTAAAG GGAACAAAAG CTGGTACCGG
            481 CCGCAAATTA AAGCCTTCGA GCGTCCCAAA ACCTTCTCAA GCAAGGTTTT CAGTATAATG
            541 TTACATGCGT ACACGCGTCT GTACAGAAAA AAAAGAAAAA TTTGAAATAT AAATAACGTT
            601 CTTAATACTA ACATAACTAT AAAAAAATAA ATAGGGACCT AGACTTCAGG TTGTCTAACT
            661 CCTTCCTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
            721 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
            781 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
            841 GGCCATCGCC NATNNGANTA NTTTGTTGAT AATGGNCTGC NNNTTGAACG GAANCNTCTT
            901 CNNCGTTGNG GNGAANNGTN AAGTTAGCTT NNANTNCNTT CNTTTGNTNG NCNGNCNNGA
          961 NGNNNACNTN GNGNNANNNN ANNNNGNNNN NNNNNTNNNN NNNNNN
          //
          Primer: paGFP-M_R1
          Region: insert mid (GFP1-10 5', TOM71 3')
          Sequence file: RDB16037_A8I3k.seq check
          >16037_16037_B0IuF_paGFP-M_R1_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNTGCCTT TTNNTGTGAT CCGGATAACG GGAAAAGCAT TGAACACCAT
             61 AGGTCAGAGT AGTGACAAGT GTTGGCCATG GAACAGGTAG TTTTCCAGTA GTGCAAATAA
            121 ATTTAAGTGT GAGTTTTCCG ATTGTAGCAT CACCTTCACC CTCTCCTCTG ACAGAAAATT
            181 TGTGCCCATT AACATCACCA TCTAATTCAA CAAGAATTGG GACAACTCCA GTGAAAAGTT
            241 CTTCTCCTTT GCTCATACTA GTGCCACCCA TTGATCCGCC ACCAGACCCT CCGGATCCAA
            301 GCATGCCTTT AGCCCTATAA CGAGCTAATG TTAGCTCCAT CTTTGCACTT ATGATTGGAT
            361 CAGCCCTCAA ACGCTTTTGT ATCTTTGCAG CTTCCGCGAA GGTAGTAGCT TGAAGTTTTT
            421 CATCCATAGT TCTTGCTAAA ATTGCAGAAT CTTCAAACAA TTCTATGGCC TCGTCAATTT
            481 TTTCCATTTG TAATTTTAGT TGTGCTAACC CGATCTTCGC TTGTTCAGAT CGTGGATCTA
            541 ATTCACAGGC TTTTGTCAGC AGCTTAATGG CTGCGTTGAA TTTCTCTTCN TCCAACTGGG
            601 TTGGATCTTG AGAGGANTGT CTAGCTAATA TAGTTGCTTT CCCNATCAAG GGTCCTATAN
            661 CCACATGTAT TTTTTCNNGN ACTTCTTCCN ANCTTTNANN NNTANCATAN NNNTNNANTN
          721 NNNNNNNNN
          //
          Primer: URA3pro_F
          Region: URA3pro, URA3
          Sequence file: RDB16037_A8I3p.seq check
          >D05803_A1_A8I3_1_URA3pro_F_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNANNN TGANNTGCCN NGTATTCTTA ACCCAACTGC ACAGAACAAA
             61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
            121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
            181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
            241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
            301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
            361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
            421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
            481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
            541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
            601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
            661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
            721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
            781 TGTTGGAAGA NGANTATTTG CAAAGGGAAN GGATGCTAAN GTAGAGGGTG AACGTTACAG
            841 AAAAGCANGC TGGGGAANCA TATTTGANAA NATGCGGCCA GCAAAACTAA AAAANNTGTA
          901 TTATAAGTAA ATGNNTGTAT ACTAAANNCN NNANNTANNN CTTNNNTTTN NNNNNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, TOM71 5')
          Sequence file: RDB16037_A8I3m.seq check
          >16037_16037_B0IuF_TDH3pro_F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNGATTG NAATTCTGTA AATCTATTTC TTAAACTTCT TAAATTCTAC
             61 TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATGCGG
            121 CCGCATGGCC GAAAACTCCC TCCTGAGGTT TATCACCAAG AACAAAGTGG CCATCCTAGC
            181 AACGGTCTCT GCGGGGACTG CTGCTGTGGG TGCTTATGTT TACTACCAGC AAATTAAGCA
            241 GCAGCAACAG CAGCAATTGA AGGGTACAAA AGACAACCGA CGTCAAAGTG AGGCCTTTGC
            301 TGGTCAAAAT GAAGACGAGG CGGACTTAAA GGACGACGGC AGCGTCGTAA GTGGAAGTAA
            361 TAAGAGGAAG AAGAAGAAGA ATAAACGCAA GAGGAACAAT AAGGCAAAAT CGGGAGAAGG
            421 GTTTGACTAT CCCTCCTTGC CCAATGGCGA GCCTGATATT GCCCAATTGA AAGGCCTATC
            481 ACCCTCCCAG AGACAAGCGT ATGCCGTGCA ACTGAAGAAC AGAGGTAACC ACTTCTTCAC
            541 TGCTAAGAAT TTTAATGAGG CCATCAAATA CTACCAGTAT GCCATTGAGC TGGANCCGAA
            601 TGANCCAGTG TTCTATTCCA ACATATCTGC TTGTTACATT TCTACNGGCG ANTTANAGAA
            661 NNNTATAGAG NTCACTACAA ANGNNNNTGA NANAAAACCT GANNNTTCNA ANNNNNGNNN
          721 ANNNNNNNNN N
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP1-10)
          Sequence file: RDB16037_A8I3q.seq check
          >D05803_A1_A8I3_1_CYC1ter_R_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GANATAACTA
             61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ACTTTTCGTT
            121 GGGATCTTTC GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG
            181 GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGG AACCATCTTC
            241 AACGTTGTGG CGAACTGTGA AGTTAGCTTT GATTCCATTC TTTTGTTTGT CTGCCGTGAT
            301 GTATACATTG TGTGAGTTAA AGTTGTACTC GAGTTTGTGT CCGAGAATGT TTCCATCTTC
            361 TTTAAAATCA GTACCCTTTA ACTCGATACG ATTAACAAGG GTATCACCTT CAAACTTGAC
            421 TACAGCACGC GTCTTGTATT TCCCGTCATC TTTGAAAGAT ATAGTGCGTT CCTGTACATA
            481 ACCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCCTTTTC ATGTGATCCG GATAACGGGA
            541 AAAGCATTGA ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT
            601 TCCAGTAGTG CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC
            661 TCCTCTGACA GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC
            721 AACTCCAGTG AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCCATTG ATCCGCCACC
            781 AGACCCTCCG GATCCNAGCA TGCCTTTAGC CCTATAACGA GCTAATGTTA NCTCCATCNT
            841 TGCACTTATG ATTGGATCAG CCCTCAAANG CTTTTTGTAT CTTNNNAGCT TNCNCNAANG
          901 NANNANCTNG AAGTTTTTNC ATCCNTNNTN NNNGCTAAAN NNNNNNNNN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB16037_A8I3r.seq check
          >D05803_A1_A8I3_1_Amp_R_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN CANTTATNNG GGTTATTGTC TCATGAGCGG ATACATATTT
             61 GAATGTATTT NNNNNAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
            121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
            181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
            241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
            301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
            361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
            421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
            481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
            541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
            601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
            661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
            721 GATGACGGTG AAAANCTCTG ACACATGCAG CTCCCGGANA CGGTCACAGC TTGTCTGTAA
            781 GCGGATGCCG GGANCAGANA AGCCCGTCAG GGCGCGTCAG CGGGNGTTGG CGGGNGTCGG
            841 GGCTGGCTTA ACNATGCNNC ATCAGANCAG ANTGTACTGA GAGNGNANCA TACCNCCTTT
            901 TCNNNTCNNN NNTTTTTTTT TTTNANTCNT TTTTTTNNNN TNCGGTTTNC NNTNNAAANT
          961 TTTTTTNNNN TNNNGNNANN NNNCNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl