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Tom71-GFP1-10

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Catalog number RDB16037
Resource name Tom71-GFP1-10
Alternative name pRS316-GPDp-Tom71-GFP1-10 (pSFL22)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae TOM71 cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae TOM71 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16037 Tom71-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16037_A8I3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: insert 5' (T7 pro, GPD pro)
Sequence file: RDB16037_A8I3i.seq check
>16037_16037_B0IuF_HB-Nos_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNN NTGGGTNNNG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA
   61 ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CAGTTTATCA
  121 TTATCAATAC TCGCCATTTC AAAGAATACG TAAATAATTA ATAGTAGTGA TTTTCCTAAC
  181 TTTATTTAGT CAAAAAATTA GCCTTTTAAT TCTGCTGTAA CCCGTACATG CCCAAAATAG
  241 GGGGCGGGTT ACACAGAATA TATAACATCG TAGGTGTCTG GGTGAACAGT TTATTCCTGG
  301 CATCCACTAA ATATAATGGA GCCCGCTTTT TAAGCTGGCA TCCAGAAAAA AAAAGAATCC
  361 CAGCACCAAA ATATTGTTTT CTTCACCAAC CATCAGTTCA TAGGTCCATT CTCTTAGCGC
  421 AACTACAGAG AACAGGGGCA CAAACAGGCA AAAAACGGGC ACAACCTCAA TGGAGTGATG
  481 CAACCTGCCT GGAGTAAATG ATGACACAAG GCAATTGACC CACGCATGTA TCTATCTCAT
  541 TTTCTTACAC CTTCTATTAC CTTCTGCTCT CTCTGATTTG GAAAAAGCTG AAAAAAAAGG
  601 NTGAAACCAG TTCCCTGAAA TTATNNNNTA CTTGACTAAT AAGTATATAA AGACNGNANG
  661 TANTGANTGT AANTCTGNAN NCNATTTCNN NAACTTNNTN AANNCNNNNT TNNNNNNNNN
721 N
//
Primer: SV40pA3'Element Reverse
Region: insert 3' (T3 pro, CYC1 ter, GFP1-10 3')
Sequence file: RDB16037_A8I3j.seq check
>16037_16037_B0IuF_SV40pA3'elementReverse_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCNNGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
   61 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
  121 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
  181 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
  241 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
  301 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
  361 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG
  421 ACCATGATTA CGCCAAGCTC GAAATTAACC CTCACTAAAG GGAACAAAAG CTGGTACCGG
  481 CCGCAAATTA AAGCCTTCGA GCGTCCCAAA ACCTTCTCAA GCAAGGTTTT CAGTATAATG
  541 TTACATGCGT ACACGCGTCT GTACAGAAAA AAAAGAAAAA TTTGAAATAT AAATAACGTT
  601 CTTAATACTA ACATAACTAT AAAAAAATAA ATAGGGACCT AGACTTCAGG TTGTCTAACT
  661 CCTTCCTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT
  721 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
  781 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
  841 GGCCATCGCC NATNNGANTA NTTTGTTGAT AATGGNCTGC NNNTTGAACG GAANCNTCTT
  901 CNNCGTTGNG GNGAANNGTN AAGTTAGCTT NNANTNCNTT CNTTTGNTNG NCNGNCNNGA
961 NGNNNACNTN GNGNNANNNN ANNNNGNNNN NNNNNTNNNN NNNNNN
//
Primer: paGFP-M_R1
Region: insert mid (GFP1-10 5', TOM71 3')
Sequence file: RDB16037_A8I3k.seq check
>16037_16037_B0IuF_paGFP-M_R1_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNTGCCTT TTNNTGTGAT CCGGATAACG GGAAAAGCAT TGAACACCAT
   61 AGGTCAGAGT AGTGACAAGT GTTGGCCATG GAACAGGTAG TTTTCCAGTA GTGCAAATAA
  121 ATTTAAGTGT GAGTTTTCCG ATTGTAGCAT CACCTTCACC CTCTCCTCTG ACAGAAAATT
  181 TGTGCCCATT AACATCACCA TCTAATTCAA CAAGAATTGG GACAACTCCA GTGAAAAGTT
  241 CTTCTCCTTT GCTCATACTA GTGCCACCCA TTGATCCGCC ACCAGACCCT CCGGATCCAA
  301 GCATGCCTTT AGCCCTATAA CGAGCTAATG TTAGCTCCAT CTTTGCACTT ATGATTGGAT
  361 CAGCCCTCAA ACGCTTTTGT ATCTTTGCAG CTTCCGCGAA GGTAGTAGCT TGAAGTTTTT
  421 CATCCATAGT TCTTGCTAAA ATTGCAGAAT CTTCAAACAA TTCTATGGCC TCGTCAATTT
  481 TTTCCATTTG TAATTTTAGT TGTGCTAACC CGATCTTCGC TTGTTCAGAT CGTGGATCTA
  541 ATTCACAGGC TTTTGTCAGC AGCTTAATGG CTGCGTTGAA TTTCTCTTCN TCCAACTGGG
  601 TTGGATCTTG AGAGGANTGT CTAGCTAATA TAGTTGCTTT CCCNATCAAG GGTCCTATAN
  661 CCACATGTAT TTTTTCNNGN ACTTCTTCCN ANCTTTNANN NNTANCATAN NNNTNNANTN
721 NNNNNNNNN
//
Primer: URA3pro_F
Region: URA3pro, URA3
Sequence file: RDB16037_A8I3l.seq check
>D05803_A1_A8I3_1_URA3pro_F_C01_07_ABI24.ab1
    1 CAAATGTAGT GTTGAGAACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA
   61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
  121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
  181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
  241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
  301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
  361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
  421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
  481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
  541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
  601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
  661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
  721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
  781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG
  841 AAAAGCAGGC TGGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA
  901 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG
  961 TTTATTACCC TATGCGGTGT GAATACCGCA CAGATGCGTA AAGGAGAAAA ATACCCGCAT
 1021 TCAGAATTGT AAGCGGTATA TTTTGTTAAA ATTCGCGTTA ATTTTTGGTA AATTCAGCTC
1081 AATTTTTTTA AGTCA
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, TOM71 5')
Sequence file: RDB16037_A8I3m.seq check
>16037_16037_B0IuF_TDH3pro_F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNGATTG NAATTCTGTA AATCTATTTC TTAAACTTCT TAAATTCTAC
   61 TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATGCGG
  121 CCGCATGGCC GAAAACTCCC TCCTGAGGTT TATCACCAAG AACAAAGTGG CCATCCTAGC
  181 AACGGTCTCT GCGGGGACTG CTGCTGTGGG TGCTTATGTT TACTACCAGC AAATTAAGCA
  241 GCAGCAACAG CAGCAATTGA AGGGTACAAA AGACAACCGA CGTCAAAGTG AGGCCTTTGC
  301 TGGTCAAAAT GAAGACGAGG CGGACTTAAA GGACGACGGC AGCGTCGTAA GTGGAAGTAA
  361 TAAGAGGAAG AAGAAGAAGA ATAAACGCAA GAGGAACAAT AAGGCAAAAT CGGGAGAAGG
  421 GTTTGACTAT CCCTCCTTGC CCAATGGCGA GCCTGATATT GCCCAATTGA AAGGCCTATC
  481 ACCCTCCCAG AGACAAGCGT ATGCCGTGCA ACTGAAGAAC AGAGGTAACC ACTTCTTCAC
  541 TGCTAAGAAT TTTAATGAGG CCATCAAATA CTACCAGTAT GCCATTGAGC TGGANCCGAA
  601 TGANCCAGTG TTCTATTCCA ACATATCTGC TTGTTACATT TCTACNGGCG ANTTANAGAA
  661 NNNTATAGAG NTCACTACAA ANGNNNNTGA NANAAAACCT GANNNTTCNA ANNNNNGNNN
721 ANNNNNNNNN N
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, GFP1-10 3')
Sequence file: RDB16037_A8I3n.seq check
>D05803_A1_A8I3_1_CYC1ter_R_A01_01_ABI24.ab1
    1 ATTTCTTTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ACTTTTCGTT
  121 GGGATCTTTC GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG
  181 GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGG AACCATCTTC
  241 AACGTTGTGG CGAACTGTGA AGTTAGCTTT GATTCCATTC TTTTGTTTGT CTGCCGTGAT
  301 GTATACATTG TGTGAGTTAA AGTTGTACTC GAGTTTGTGT CCGAGAATGT TTCCATCTTC
  361 TTTAAAATCA GTACCCTTTA ACTCGATACG ATTAACAAGG GTATCACCTT CAAACTTGAC
  421 TACAGCACGC GTCTTGTATT TCCCGTCATC TTTGAAAGAT ATAGTGCGTT CCTGTACATA
  481 ACCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCCTTTTC ATGTGATCCG GATAACGGGA
  541 AAAGCATTGA ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT
  601 TCCAGTAGTG CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC
  661 TCCTCTGACA GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC
  721 AACTCCAGTG AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCCATTG ATCCGCCACC
  781 AGACCCTCCG GATCCAAGCA TGCCTTTAGC CCTATAACGA GCTAATGTTA GCTCCATCTT
  841 TGCACTTATG ATTGGATCAG CCCTCAAACG CTTTTTGTAT CTTTGCAGCT TCCGCGAAGG
  901 TAGTAGCTTG AAGTTTTTTC ATCCATAGTT CTTGCTAAAA TTGCAGAATC TTCAAACAAA
  961 TTCTATGGCC CTCGTCAATT TTTTTTCCCA TTTGTAAATT TTAGTTTGTG CCTACCCGAT
 1021 CTTTCGCTTG TTTCAGATCG TTGGATCCTA AATTTCACAG GCTCTGTCAG CAGCTTGATG
1081 ACCTGCGATT GCAT
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS, URA3 pro
Sequence file: RDB16037_A8I3o.seq check
>D05803_A1_A8I3_1_Amp_R_D01_10_ABI24.ab1
    1 TCTTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
  781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG
  841 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TACCACCTTT
  901 TCAATTCATC ATTTTTTTTT TTTTATTCTT TTTTTTTGAT TTCGGTTTCC CTTTGAAATT
  961 TTTTTTTGAT TTCGGTAAAT CTCCCGAACA GAAGGGAAGG AACGAATGGT GGAGCACAGA
 1021 CTTTAGATTT GGTATATAAT ACGCATATGT AGGTGCTGAG AAACATGAAA TGCCCAGTAT
1081 CGTAACCGAA CTGCACAGAA CCAAAAACCG TTGCAGGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.11.06

GNP_filter3_RDBDEP_html_201104.pl