Please review the QC test results indicated by icon below as well as clone information before placing your order.

Tom71-GFP1-10

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16037_A8I3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R Region: insert 5' (T7 pro, GPD pro) Sequence file: RDB16037_A8I3i.seq
>16037_16037_B0IuF_HB-Nos_R_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNGNNNNNN NTGGGTNNNG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA    61 ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CAGTTTATCA   121 TTATCAATAC TCGCCATTTC AAAGAATACG TAAATAATTA ATAGTAGTGA TTTTCCTAAC   181 TTTATTTAGT CAAAAAATTA GCCTTTTAAT TCTGCTGTAA CCCGTACATG CCCAAAATAG   241 GGGGCGGGTT ACACAGAATA TATAACATCG TAGGTGTCTG GGTGAACAGT TTATTCCTGG   301 CATCCACTAA ATATAATGGA GCCCGCTTTT TAAGCTGGCA TCCAGAAAAA AAAAGAATCC   361 CAGCACCAAA ATATTGTTTT CTTCACCAAC CATCAGTTCA TAGGTCCATT CTCTTAGCGC   421 AACTACAGAG AACAGGGGCA CAAACAGGCA AAAAACGGGC ACAACCTCAA TGGAGTGATG   481 CAACCTGCCT GGAGTAAATG ATGACACAAG GCAATTGACC CACGCATGTA TCTATCTCAT   541 TTTCTTACAC CTTCTATTAC CTTCTGCTCT CTCTGATTTG GAAAAAGCTG AAAAAAAAGG   601 NTGAAACCAG TTCCCTGAAA TTATNNNNTA CTTGACTAAT AAGTATATAA AGACNGNANG   661 TANTGANTGT AANTCTGNAN NCNATTTCNN NAACTTNNTN AANNCNNNNT TNNNNNNNNN 721 N //
Primer: SV40pA3'Element Reverse Region: insert 3' (T3 pro, CYC1 ter, GFP1-10 3') Sequence file: RDB16037_A8I3j.seq
>16037_16037_B0IuF_SV40pA3'elementReverse_G05_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCNNGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT    61 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA   121 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT   181 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC   241 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA   301 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC   361 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG   421 ACCATGATTA CGCCAAGCTC GAAATTAACC CTCACTAAAG GGAACAAAAG CTGGTACCGG   481 CCGCAAATTA AAGCCTTCGA GCGTCCCAAA ACCTTCTCAA GCAAGGTTTT CAGTATAATG   541 TTACATGCGT ACACGCGTCT GTACAGAAAA AAAAGAAAAA TTTGAAATAT AAATAACGTT   601 CTTAATACTA ACATAACTAT AAAAAAATAA ATAGGGACCT AGACTTCAGG TTGTCTAACT   661 CCTTCCTTTT CGGTTAGAGC GGATGTGGGG GGAGGGCGTG AATGTAAGCG TGACATAACT   721 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT   781 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG   841 GGCCATCGCC NATNNGANTA NTTTGTTGAT AATGGNCTGC NNNTTGAACG GAANCNTCTT   901 CNNCGTTGNG GNGAANNGTN AAGTTAGCTT NNANTNCNTT CNTTTGNTNG NCNGNCNNGA 961 NGNNNACNTN GNGNNANNNN ANNNNGNNNN NNNNNTNNNN NNNNNN //
Primer: paGFP-M_R1 Region: insert mid (GFP1-10 5', TOM71 3') Sequence file: RDB16037_A8I3k.seq
>16037_16037_B0IuF_paGFP-M_R1_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNTGCCTT TTNNTGTGAT CCGGATAACG GGAAAAGCAT TGAACACCAT    61 AGGTCAGAGT AGTGACAAGT GTTGGCCATG GAACAGGTAG TTTTCCAGTA GTGCAAATAA   121 ATTTAAGTGT GAGTTTTCCG ATTGTAGCAT CACCTTCACC CTCTCCTCTG ACAGAAAATT   181 TGTGCCCATT AACATCACCA TCTAATTCAA CAAGAATTGG GACAACTCCA GTGAAAAGTT   241 CTTCTCCTTT GCTCATACTA GTGCCACCCA TTGATCCGCC ACCAGACCCT CCGGATCCAA   301 GCATGCCTTT AGCCCTATAA CGAGCTAATG TTAGCTCCAT CTTTGCACTT ATGATTGGAT   361 CAGCCCTCAA ACGCTTTTGT ATCTTTGCAG CTTCCGCGAA GGTAGTAGCT TGAAGTTTTT   421 CATCCATAGT TCTTGCTAAA ATTGCAGAAT CTTCAAACAA TTCTATGGCC TCGTCAATTT   481 TTTCCATTTG TAATTTTAGT TGTGCTAACC CGATCTTCGC TTGTTCAGAT CGTGGATCTA   541 ATTCACAGGC TTTTGTCAGC AGCTTAATGG CTGCGTTGAA TTTCTCTTCN TCCAACTGGG   601 TTGGATCTTG AGAGGANTGT CTAGCTAATA TAGTTGCTTT CCCNATCAAG GGTCCTATAN   661 CCACATGTAT TTTTTCNNGN ACTTCTTCCN ANCTTTNANN NNTANCATAN NNNTNNANTN 721 NNNNNNNNN //
Primer: URA3pro_F Region: URA3pro, URA3 Sequence file: RDB16037_A8I3l.seq
>D05803_A1_A8I3_1_URA3pro_F_C01_07_ABI24.ab1     1 CAAATGTAGT GTTGAGAACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA    61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC   121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT   181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG   241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG   301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG   361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT   421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG   481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA   541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC   601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA   661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC   721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT   781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG   841 AAAAGCAGGC TGGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA   901 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG   961 TTTATTACCC TATGCGGTGT GAATACCGCA CAGATGCGTA AAGGAGAAAA ATACCCGCAT  1021 TCAGAATTGT AAGCGGTATA TTTTGTTAAA ATTCGCGTTA ATTTTTGGTA AATTCAGCTC 1081 AATTTTTTTA AGTCA //
Primer: TDH3pro_F Region: insert mid (GPD pro, TOM71 5') Sequence file: RDB16037_A8I3m.seq
>16037_16037_B0IuF_TDH3pro_F_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNGATTG NAATTCTGTA AATCTATTTC TTAAACTTCT TAAATTCTAC    61 TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATGCGG   121 CCGCATGGCC GAAAACTCCC TCCTGAGGTT TATCACCAAG AACAAAGTGG CCATCCTAGC   181 AACGGTCTCT GCGGGGACTG CTGCTGTGGG TGCTTATGTT TACTACCAGC AAATTAAGCA   241 GCAGCAACAG CAGCAATTGA AGGGTACAAA AGACAACCGA CGTCAAAGTG AGGCCTTTGC   301 TGGTCAAAAT GAAGACGAGG CGGACTTAAA GGACGACGGC AGCGTCGTAA GTGGAAGTAA   361 TAAGAGGAAG AAGAAGAAGA ATAAACGCAA GAGGAACAAT AAGGCAAAAT CGGGAGAAGG   421 GTTTGACTAT CCCTCCTTGC CCAATGGCGA GCCTGATATT GCCCAATTGA AAGGCCTATC   481 ACCCTCCCAG AGACAAGCGT ATGCCGTGCA ACTGAAGAAC AGAGGTAACC ACTTCTTCAC   541 TGCTAAGAAT TTTAATGAGG CCATCAAATA CTACCAGTAT GCCATTGAGC TGGANCCGAA   601 TGANCCAGTG TTCTATTCCA ACATATCTGC TTGTTACATT TCTACNGGCG ANTTANAGAA   661 NNNTATAGAG NTCACTACAA ANGNNNNTGA NANAAAACCT GANNNTTCNA ANNNNNGNNN 721 ANNNNNNNNN N //
Primer: CYC1ter_R Region: insert mid (CYC1 ter, GFP1-10 3') Sequence file: RDB16037_A8I3n.seq
>D05803_A1_A8I3_1_CYC1ter_R_A01_01_ABI24.ab1     1 ATTTCTTTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT GACATAACTA    61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ACTTTTCGTT   121 GGGATCTTTC GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG   181 GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGG AACCATCTTC   241 AACGTTGTGG CGAACTGTGA AGTTAGCTTT GATTCCATTC TTTTGTTTGT CTGCCGTGAT   301 GTATACATTG TGTGAGTTAA AGTTGTACTC GAGTTTGTGT CCGAGAATGT TTCCATCTTC   361 TTTAAAATCA GTACCCTTTA ACTCGATACG ATTAACAAGG GTATCACCTT CAAACTTGAC   421 TACAGCACGC GTCTTGTATT TCCCGTCATC TTTGAAAGAT ATAGTGCGTT CCTGTACATA   481 ACCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCCTTTTC ATGTGATCCG GATAACGGGA   541 AAAGCATTGA ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT   601 TCCAGTAGTG CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC   661 TCCTCTGACA GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC   721 AACTCCAGTG AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCCATTG ATCCGCCACC   781 AGACCCTCCG GATCCAAGCA TGCCTTTAGC CCTATAACGA GCTAATGTTA GCTCCATCTT   841 TGCACTTATG ATTGGATCAG CCCTCAAACG CTTTTTGTAT CTTTGCAGCT TCCGCGAAGG   901 TAGTAGCTTG AAGTTTTTTC ATCCATAGTT CTTGCTAAAA TTGCAGAATC TTCAAACAAA   961 TTCTATGGCC CTCGTCAATT TTTTTTCCCA TTTGTAAATT TTAGTTTGTG CCTACCCGAT  1021 CTTTCGCTTG TTTCAGATCG TTGGATCCTA AATTTCACAG GCTCTGTCAG CAGCTTGATG 1081 ACCTGCGATT GCAT //
Primer: Amp_R Region: AmpR pro, CEN_ARS, URA3 pro Sequence file: RDB16037_A8I3o.seq
>D05803_A1_A8I3_1_Amp_R_D01_10_ABI24.ab1     1 TCTTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA   181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC   241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC   301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA   361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT   421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT   481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC   541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA   601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC   661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT   721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA   781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG   841 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TACCACCTTT   901 TCAATTCATC ATTTTTTTTT TTTTATTCTT TTTTTTTGAT TTCGGTTTCC CTTTGAAATT   961 TTTTTTTGAT TTCGGTAAAT CTCCCGAACA GAAGGGAAGG AACGAATGGT GGAGCACAGA  1021 CTTTAGATTT GGTATATAAT ACGCATATGT AGGTGCTGAG AAACATGAAA TGCCCAGTAT 1081 CGTAACCGAA CTGCACAGAA CCAAAAACCG TTGCAGGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.11.06

GNP_filter3_RDBDEP_html_201104.pl