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Tom71-GFP1-10

Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo.

Catalog number RDB16037
Resource name Tom71-GFP1-10
Alternative name pRS316-GPDp-Tom71-GFP1-10 (pSFL22)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name S. cerevisiae TOM71 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae TOM71 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB16037 Tom71-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16037_A8I3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Region: GPD pro, insert 5'
Sequence file: RDB16037_A8I3e.seq check
>D05803_A1_A8I3_1_M13_-40_A10_01_ABI24.ab1
    1 AACAAGGCAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT
   61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA
  121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG
  181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT
  241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA
  301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG
  361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC
  421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC
  481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC
  541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT
  601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAaATTCT ACTTTTATAG TTAGTCTTTT
  661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGG CCGAAAACTC
  721 CCTCCTGAGG TTTATCACCA AGAACAAAGT GGCCATCCTA GCAACGGTCT CTGCGGGGAC
  781 TGCTGCTGTG GGTGCTTATG TTTACTACCA GCAAATTAAG CAGCAGCAAC AGCAGCAATT
  841 GAAGGGTACA AAAGACAACC CGACGTCAAA GTGAGGCCTT TGCTGGGTCA AATGAAGACG
  901 AGGCGGACTT AAAGGACGAC GGCAGCGTCG TAGTGAGTAT AGAGAGAGGA GAGATAACGC
  961 AGAGACATAG CAAATCGGAG AACGTTGACT ATCCCTCCTG CCAATTGGCG AGCCTGGAAT
1021 TGGCCAGATG AAGCCTATAC TCCTCCTGAG AACAGCCGA
//
Primer: CYC1ter_R
Region: CYC1 ter, insert 3'
Sequence file: RDB16037_A8I3f.seq check
>D05803_A1_A8I3_1_CYC1ter_R_A01_01_ABI24.ab1
    1 ATTTCTTTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ACTTTTCGTT
  121 GGGATCTTTC GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG
  181 GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGG AACCATCTTC
  241 AACGTTGTGG CGAACTGTGA AGTTAGCTTT GATTCCATTC TTTTGTTTGT CTGCCGTGAT
  301 GTATACATTG TGTGAGTTAA AGTTGTACTC GAGTTTGTGT CCGAGAATGT TTCCATCTTC
  361 TTTAAAATCA GTACCCTTTA ACTCGATACG ATTAACAAGG GTATCACCTT CAAACTTGAC
  421 TACAGCACGC GTCTTGTATT TCCCGTCATC TTTGAAAGAT ATAGTGCGTT CCTGTACATA
  481 ACCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCCTTTTC ATGTGATCCG GATAACGGGA
  541 AAAGCATTGA ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT
  601 TCCAGTAGTG CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC
  661 TCCTCTGACA GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC
  721 AACTCCAGTG AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCCATTG ATCCGCCACC
  781 AGACCCTCCG GATCCAAGCA TGCCTTTAGC CCTATAACGA GCTAATGTTA GCTCCATCTT
  841 TGCACTTATG ATTGGATCAG CCCTCAAACG CTTTTTGTAT CTTTGCAGCT TCCGCGAAGG
  901 TAGTAGCTTG AAGTTTTTTC ATCCATAGTT CTTGCTAAAA TTGCAGAATC TTCAAACAAA
  961 TTCTATGGCC CTCGTCAATT TTTTTTCCCA TTTGTAAATT TTAGTTTGTG CCTACCCGAT
 1021 CTTTCGCTTG TTTCAGATCG TTGGATCCTA AATTTCACAG GCTCTGTCAG CAGCTTGATG
1081 ACCTGCGATT GCAT
//
Primer: URA3pro_F
Region: URA3
Sequence file: RDB16037_A8I3g.seq check
>D05803_A1_A8I3_1_URA3pro_F_C01_07_ABI24.ab1
    1 CAAATGTAGT GTTGAGAACA TGAATTGCCC AGTATTCTTA ACCCAACTGC ACAGAACAAA
   61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
  121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
  181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
  241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
  301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
  361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
  421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
  481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
  541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
  601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
  661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
  721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA TGTGGTCTCT ACAGGATCTG ACATTATTAT
  781 TGTTGGAAGA GGACTATTTG CAAAGGGAAG GGATGCTAAG GTAGAGGGTG AACGTTACAG
  841 AAAAGCAGGC TGGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA
  901 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG
  961 TTTATTACCC TATGCGGTGT GAATACCGCA CAGATGCGTA AAGGAGAAAA ATACCCGCAT
 1021 TCAGAATTGT AAGCGGTATA TTTTGTTAAA ATTCGCGTTA ATTTTTGGTA AATTCAGCTC
1081 AATTTTTTTA AGTCA
//
Primer: Amp_R
Region: CEN/ARS
Sequence file: RDB16037_A8I3h.seq check
>D05803_A1_A8I3_1_Amp_R_D01_10_ABI24.ab1
    1 TCTTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
  781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG
  841 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TACCACCTTT
  901 TCAATTCATC ATTTTTTTTT TTTTATTCTT TTTTTTTGAT TTCGGTTTCC CTTTGAAATT
  961 TTTTTTTGAT TTCGGTAAAT CTCCCGAACA GAAGGGAAGG AACGAATGGT GGAGCACAGA
 1021 CTTTAGATTT GGTATATAAT ACGCATATGT AGGTGCTGAG AAACATGAAA TGCCCAGTAT
1081 CGTAACCGAA CTGCACAGAA CCAAAAACCG TTGCAGGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl