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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCX-rRobo2-Fc

Expression vector of the extracellular domain of rat slit receptor tagged with the Fc region of human IgG (Robo2-Fc).

Catalog number RDB16033
Resource name pCX-rRobo2-Fc
Clone info. Expression vector of the extracellular domain of rat slit receptor, which was tagged with the Fc region of human IgG (Robo2-Fc).
Vector backbone pCX (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name rat Robo2 cDNA
Depositor|Developer Masu, Masayuki |
Other clones in our bank

External Database
rat Robo2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 7(1), 13847, 2017) RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Tsukuba University. (chizai@ilc.tsukuba.ac.jp) RECIPIENT must contact the DEPOSITOR (chizai@ilc.tsukuba.ac.jp) in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16033 pCX-rRobo2-Fc DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Okada, T., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16033_A8Htp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V3
          Region: chimeric intron, insert 5'(rRobo2 5')
          Sequence file: RDB16033_A8Hte.seq check
          >D05660B7-2_A8Ht_pCAG-F_V3_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNT TNNNNNNNNN NNNNNNNAGG GCGGGGTTCG GCTTCTGGCG
             61 TGTGANCGGC GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA
            121 GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTTCGAGG
            181 TCGACGGTAT CGATAAGCTT GATATCGAAT TCCTGCAGCC CAGTCTAAAC TTCGGACCTA
            241 CCTCCTTTTT TGATACTTAT TTTTGTACTT CTGCTCTCTG GGATTGGTTT CTTAAACAAC
            301 CTGGATCCTT TTTCATATGT CAAAATGACT CCTCTGATGT TTACACTACT ATTGCTCTTT
            361 GGATTTCTCT GCATTCGGAC TGACGGATCT CGTCTTCGTC AAGAAGACTT TCCCCCCAGG
            421 ATCGTAGAAC ACCCTTCTGA CGTCATCGTC TCCAAGGGAG AGCCCACCAC TCTGAACTGT
            481 AAAGCAGAGG GCCGGCCCAC TCCCACCATT GAATGGTACA AGGATGGTGA GCGGGTGGAG
            541 ACAGACAAGG ATGATCCCAG GTCACACAGG ATGCTTCTGC CCAGCGGATC CTTATTCTTT
            601 TTGCGAATTG TTCACGGACG CAGAAGTAAA CCAGATGAAG GAACTTACGT CTGTGTTGCA
            661 AGGAACTATC TTGGTGAAGC AGTGAGTCGC AATGCATCTC TGGAAGTAGC ATTATTGCGA
            721 GATGACTTCC GGCAAAACCC CACAGATGTG GTAGTCGCAN CANGAGAGCC TGCANTCTTG
            781 GAGTGCCAGC CACCACGGGG ACACCCNNNA CCAACCATCT ACTGNAAAAA GGACNAAGTC
            841 CGAATTGATG ANAAGGANNA GAGAATAAGT ATCCGTGGTG GGAAGCTGAT GATCTCCNAT
            901 ACTAGGAAAA NCGATGCTGG CNTGTATACC TGNNTGGGGA ANCNNNNNTG GNGGGNANAA
          961 AGGNANNNCN ANNCTGCNNN NNTNNCNNNT CTTNNANCNA CNNNNNNNNN N
          //
          Primer: pCAGGS_R1
          Region: beta-globin pA,insert 3'(hIgG Fc 3')
          Sequence file: RDB16033_A8Htf.seq check
          >16033_16033_B1JdF_pCAGGS_R1_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNCNNT TNNNNNNAAA ATAAATTTCC TTTATTAGCC AGAAGTCAGA
             61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCTCAGTGG
            121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC
            181 TGAGGAGTGA ATTTCGAGCA GCTGAAGCTT TCATTTACCC GGAGACAGGG AGAGGCTCTT
            241 CTGCGTGTAG TGGTTGTGCA GAGCCTCATG CATCACGGAG CATGAGAAGA CGTTCCCCTG
            301 CTGCCACCTG CTCTTGTCCA CGGTGAGCTT GCTGTAGAGG AAGAAGGAGC CGTCGGAGTC
            361 CAGCACGGGA GGCGTGGTCT TGTAGTTGTT CTCCGGCTGC CCATTGCTCT CCCACTCCAC
            421 GGCGATGTCG CTGGGATAGA AGCCTTTGAC CAGGCAGGTC AGGCTGACCT GGTTCTTGGT
            481 CAGCTCATCC CGGGATGGGG GCAGGGTGTA CACCTGTGGT TCTCGGGGCT GCCCTTTGGC
            541 TTTGGAGANG GTTTTCTCGA TGGGGGCTGG GANGGCTTTG TTGGAGACCT TGCACTTGTA
            601 CTCCTTGNCA TTCNGCCNGT CCTGGNGCAN GANGGTGANG ANGCTGANNN CNCNGTACNT
            661 GCTGNTGNAC NGNTNCTCCC GCGGCTTTGN CNNNGNNNNN TGNNNNTNCN NNNNNNCNNN
          721 NNANNNNNNN NNN
          //
          Primer: rRobo2-M_F
          Region: insert mid(rRobo2 3',hIgG Fc 5')
          Sequence file: RDB16033_A8Htg.seq check
          >16033_16033_B1JdF_rRobo2-M_F_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNGG NGGNNCGACN ACCCCNGTTT ATTCNGGGCT ACAGAGTGAT
             61 GTACCGACAG ACTTCTGGTC TACAAGCTTC AACTGTGTGG CAGAATCTAG ATGCCAAAGT
            121 CCCGACTGAG AGGAGTGCTG TCCTTGTGAA TCTGAAAAAG GGGGTGACTT ATGAAATTAA
            181 AGTCAGGCCA TATTTCAATG AGTTCCAAGG AATGGACAGT GAATCAAAAA CAATCCGTAC
            241 TACTGAGGAA GCTCCAAGTG CGCCTCCCCA GTCTGTCACG GTGCTGACCG TTGGAAGCCA
            301 TAACAGCACA AGCATTAGTG TTTCCTGGGA TCCTCCACCA GCCGACCACC AGAATGGAAT
            361 CATACAGGAA TACAGATCTG GTCGACTGAT GCTTGGTCCC GGGCTCGACA AAACTCACAC
            421 ATGCCCACCG TGCCCAGCAC CTGAACTCCT GGGGGGACCG TCAGTCTTCC TCTTCCCCCC
            481 AAAACCCAAG GACACCCTCA TGATCTCCCG GACCCCTGAG GTCACATGCG TGGTGGTGGA
            541 CGTGAGCCAC GAAGANCCTG AGGTCAAGTT CAACTGGTAC GTGGACGGCG TGGANGTGCA
            601 TAATGNCNAG ACAAAGCCGC GGGANGAGCA NTACNACAGC ACGTACCGTG TGGTCNGCGT
            661 CCTCNCCGTC CTGCANNANG NNNGGNTGNA TGNNNNNNAN TANNNNNGNN NNNNCNNNNN
          721 NNNCNNNNNN NCCNNNNNNN NAANNNNNNT NNNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,SV40 pA,ori
          Sequence file: RDB16033_A8Hth.seq check
          >16033_16033_B1JdF_SV40pro_ori_F_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNGCCGC CTCNGCCTCT GANCTATTCC NGAAGTAGTG AGGAGGCTTT
             61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
            121 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
            181 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCCGCTGC ATTAATGAAT
            241 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
            301 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
            361 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
            421 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
            481 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
            541 ATAAAGATAC CAGGCGTTTC CCCCTGGAAN CTCCCTCGTG CGCTCTCCTN NTNNCGACCC
            601 NGCCGCTTAC CGGANACCTG TCCGCCNTTC NCCCTTCNGG AAGCGTNNNG CTTTCTNNTA
            661 NCTNACGCTG NNNNATCTCA NTTCGGNGNN NNCGNNNNNT NNNNNNNGGG NNGNGNGNNN
          721 NNNNNNCCNN NNNNNNCNNA NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Okada, T., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl