Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCX-rRobo2-Fc (#RDB16033)

Expression vector of the extracellular domain of rat slit receptor tagged with the Fc region of human IgG (Robo2-Fc).

Clone info. Expression vector of the extracellular domain of rat slit receptor, which was tagged with the Fc region of human IgG (Robo2-Fc).
Vector backbone pCX (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name rat Robo2 cDNA
Depositor|Developer Masu, Masayuki |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 7(1), 13847, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Tsukuba University. (chizai@ilc.tsukuba.ac.jp) RECIPIENT must contact the DEPOSITOR (chizai@ilc.tsukuba.ac.jp) in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 7(1), 13847, 2017)。 非営利機関が非営利目的の教育・研究用に用いる場合以外は、筑波大学と別途MTAを締結すること(chizai@ilc.tsukuba.ac.jp)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者(chizai@ilc.tsukuba.ac.jp) と別途協議を行うこと。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16033 pCX-rRobo2-Fc DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCX-rRobo2-Fc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16033).

Reference section:

Okada, T., Keino-Masu, K., Nagamine, S., Kametani, F., Ohto, T., Hasegawa, M., van Kuppevelt, T.H., Kunita, S., Takahashi, S., Masu, M., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16033_A8Htp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 (Pr0495)
Region: chimeric intron, insert 5'(rRobo2 5')
Sequence file: RDB16033_A8Hte.seq check
>D05660B7-2_A8Ht_pCAG-F_V3_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT TNNNNNNNNN NNNNNNNAGG GCGGGGTTCG GCTTCTGGCG
   61 TGTGANCGGC GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA
  121 GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTTCGAGG
  181 TCGACGGTAT CGATAAGCTT GATATCGAAT TCCTGCAGCC CAGTCTAAAC TTCGGACCTA
  241 CCTCCTTTTT TGATACTTAT TTTTGTACTT CTGCTCTCTG GGATTGGTTT CTTAAACAAC
  301 CTGGATCCTT TTTCATATGT CAAAATGACT CCTCTGATGT TTACACTACT ATTGCTCTTT
  361 GGATTTCTCT GCATTCGGAC TGACGGATCT CGTCTTCGTC AAGAAGACTT TCCCCCCAGG
  421 ATCGTAGAAC ACCCTTCTGA CGTCATCGTC TCCAAGGGAG AGCCCACCAC TCTGAACTGT
  481 AAAGCAGAGG GCCGGCCCAC TCCCACCATT GAATGGTACA AGGATGGTGA GCGGGTGGAG
  541 ACAGACAAGG ATGATCCCAG GTCACACAGG ATGCTTCTGC CCAGCGGATC CTTATTCTTT
  601 TTGCGAATTG TTCACGGACG CAGAAGTAAA CCAGATGAAG GAACTTACGT CTGTGTTGCA
  661 AGGAACTATC TTGGTGAAGC AGTGAGTCGC AATGCATCTC TGGAAGTAGC ATTATTGCGA
  721 GATGACTTCC GGCAAAACCC CACAGATGTG GTAGTCGCAN CANGAGAGCC TGCANTCTTG
  781 GAGTGCCAGC CACCACGGGG ACACCCNNNA CCAACCATCT ACTGNAAAAA GGACNAAGTC
  841 CGAATTGATG ANAAGGANNA GAGAATAAGT ATCCGTGGTG GGAAGCTGAT GATCTCCNAT
  901 ACTAGGAAAA NCGATGCTGG CNTGTATACC TGNNTGGGGA ANCNNNNNTG GNGGGNANAA
961 AGGNANNNCN ANNCTGCNNN NNTNNCNNNT CTTNNANCNA CNNNNNNNNN N
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globin pA,insert 3'(hIgG Fc 3')
Sequence file: RDB16033_A8Htf.seq check
>16033_16033_B1JdF_pCAGGS_R1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCNNT TNNNNNNAAA ATAAATTTCC TTTATTAGCC AGAAGTCAGA
   61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCTCAGTGG
  121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC
  181 TGAGGAGTGA ATTTCGAGCA GCTGAAGCTT TCATTTACCC GGAGACAGGG AGAGGCTCTT
  241 CTGCGTGTAG TGGTTGTGCA GAGCCTCATG CATCACGGAG CATGAGAAGA CGTTCCCCTG
  301 CTGCCACCTG CTCTTGTCCA CGGTGAGCTT GCTGTAGAGG AAGAAGGAGC CGTCGGAGTC
  361 CAGCACGGGA GGCGTGGTCT TGTAGTTGTT CTCCGGCTGC CCATTGCTCT CCCACTCCAC
  421 GGCGATGTCG CTGGGATAGA AGCCTTTGAC CAGGCAGGTC AGGCTGACCT GGTTCTTGGT
  481 CAGCTCATCC CGGGATGGGG GCAGGGTGTA CACCTGTGGT TCTCGGGGCT GCCCTTTGGC
  541 TTTGGAGANG GTTTTCTCGA TGGGGGCTGG GANGGCTTTG TTGGAGACCT TGCACTTGTA
  601 CTCCTTGNCA TTCNGCCNGT CCTGGNGCAN GANGGTGANG ANGCTGANNN CNCNGTACNT
  661 GCTGNTGNAC NGNTNCTCCC GCGGCTTTGN CNNNGNNNNN TGNNNNTNCN NNNNNNCNNN
721 NNANNNNNNN NNN
//
Primer: rRobo2-M_F (Pr1069)
Region: insert mid(rRobo2 3',hIgG Fc 5')
Sequence file: RDB16033_A8Htg.seq check
>16033_16033_B1JdF_rRobo2-M_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGG NGGNNCGACN ACCCCNGTTT ATTCNGGGCT ACAGAGTGAT
   61 GTACCGACAG ACTTCTGGTC TACAAGCTTC AACTGTGTGG CAGAATCTAG ATGCCAAAGT
  121 CCCGACTGAG AGGAGTGCTG TCCTTGTGAA TCTGAAAAAG GGGGTGACTT ATGAAATTAA
  181 AGTCAGGCCA TATTTCAATG AGTTCCAAGG AATGGACAGT GAATCAAAAA CAATCCGTAC
  241 TACTGAGGAA GCTCCAAGTG CGCCTCCCCA GTCTGTCACG GTGCTGACCG TTGGAAGCCA
  301 TAACAGCACA AGCATTAGTG TTTCCTGGGA TCCTCCACCA GCCGACCACC AGAATGGAAT
  361 CATACAGGAA TACAGATCTG GTCGACTGAT GCTTGGTCCC GGGCTCGACA AAACTCACAC
  421 ATGCCCACCG TGCCCAGCAC CTGAACTCCT GGGGGGACCG TCAGTCTTCC TCTTCCCCCC
  481 AAAACCCAAG GACACCCTCA TGATCTCCCG GACCCCTGAG GTCACATGCG TGGTGGTGGA
  541 CGTGAGCCAC GAAGANCCTG AGGTCAAGTT CAACTGGTAC GTGGACGGCG TGGANGTGCA
  601 TAATGNCNAG ACAAAGCCGC GGGANGAGCA NTACNACAGC ACGTACCGTG TGGTCNGCGT
  661 CCTCNCCGTC CTGCANNANG NNNGGNTGNA TGNNNNNNAN TANNNNNGNN NNNNCNNNNN
721 NNNCNNNNNN NCCNNNNNNN NAANNNNNNT NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,SV40 pA,ori
Sequence file: RDB16033_A8Hth.seq check
>16033_16033_B1JdF_SV40pro_ori_F_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNGCCGC CTCNGCCTCT GANCTATTCC NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
  121 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
  181 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCCGCTGC ATTAATGAAT
  241 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
  301 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
  361 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
  421 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
  481 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
  541 ATAAAGATAC CAGGCGTTTC CCCCTGGAAN CTCCCTCGTG CGCTCTCCTN NTNNCGACCC
  601 NGCCGCTTAC CGGANACCTG TCCGCCNTTC NCCCTTCNGG AAGCGTNNNG CTTTCTNNTA
  661 NCTNACGCTG NNNNATCTCA NTTCGGNGNN NNCGNNNNNT NNNNNNNGGG NNGNGNGNNN
721 NNNNNNCCNN NNNNNNCNNA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Okada, T., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064. [link to RRC of NBRP]