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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCX-mSlit2 (#RDB16025)

Expression vector of mouse slit guidance ligand 2 (Slit2) with a signal sequence from the human CD8A gene, the FLAG peptide and the 6xHis tag.

Clone info. Expression vector of mouse slit guidance ligand 2 (Slit2) in pCX vector containing a kozak sequence, a signal sequence from the human CD8A gene, the FLAG peptide, and the 6xHis tag.
Comment Expression was confirmed by the depositor with fluorescece imaging.
Vector backbone pCX (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name mouse Slit2 cDNA
Depositor|Developer Masu, Masayuki |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 7(1), 13847, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Tsukuba University. (chizai@ilc.tsukuba.ac.jp) RECIPIENT must contact the DEPOSITOR (chizai@ilc.tsukuba.ac.jp) in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 7(1), 13847, 2017)。 非営利機関が非営利目的の教育・研究用に用いる場合以外は、筑波大学と別途MTAを締結すること(chizai@ilc.tsukuba.ac.jp)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者(chizai@ilc.tsukuba.ac.jp) と別途協議を行うこと。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16025 pCX-mSlit2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCX-mSlit2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16025).

Reference section:

Okada, T., Keino-Masu, K., Nagamine, S., Kametani, F., Ohto, T., Hasegawa, M., van Kuppevelt, T.H., Kunita, S., Takahashi, S., Masu, M., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16025_A8Htp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 (Pr0495)
Region: signal seq,FLAG,6xHis,insert 5'
Sequence file: RDB16025_A8Htc.seq check
>D05660A8-3_A8Ht_pCAG-F_V3_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGGNNNNN GGNANGGCGG GGTTCGGCTT CTGGCGTGTG
   61 ANCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACNGCTC
  121 CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CGCGCCTGCA
  181 GGCCACCATG GCCTTACCAG TGACCGCCTT GCTCCTGCCG CTAGCCTTGC TGCTCCACGC
  241 CGCCAGGCCG GATTACAAGG ACGATGACGA CAAGCATCAT CACCACCATC ACTCTGCAGG
  301 CGGCCGCCAG GCGTGCCCGG CCCAGTGCTC CTGTTCAGGC AGCACGGTGG ACTGTCATGG
  361 GCTGGCACTG CGCAGTGTGC CCAGGAATAT CCCCCGCAAC ACCGAGAGAC TGGATTTGAA
  421 TGGAAATAAC ATCACGAGGA TCACGAAGAT AGATTTTGCT GGTCTCAGGC ACCTCAGAGT
  481 TCTTCAGCTC ATGGAGAACA GAATCAGCAC CATCGAGAGG GGAGCATTCC AGGATCTTAA
  541 GGAGCTGGAA AGACTGCGTT TAAACAGAAA TAACCTTCAG TTGTTTCCTG AGCTGCTGTT
  601 TCTCGGGACT GCGAAGCTCT ACCGGCTTGA TCTCAGTGAA AATCAAATTC AAGCAATTCC
  661 AAGGAAGGCT TTCCGTGGGG CAGTTGACAT TAAAAACCTG CAACTGGATT ACAACCAGAT
  721 CANCTGCATT GAAGATGGGG CGTTCAGAGC TCTACGANAT CTGGAAGTGC TCACTCTGAA
  781 CNATAACAAT ATTACTAGAC TTTCAGTGGC AAGTTTCAAC CATATGCCNN AAACTNAGGA
  841 CATTTCGACT CCACTCNAAN NAACTTGTAC TGCGNNTGCC ACCTANCCTG GCTCTCANAC
  901 TGGCTTCGNC NAAGGNCACG GGNGGGCTNG TACACTCANN NNNTGGGNCC NNTNCNNNCC
961 NGANGNNNNA NNATGNNNNN NANGNNTNNA ANANNNANNN NTTNNNNNNN NNN
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globin pA,insert 3'
Sequence file: RDB16025_A8Htd.seq check
>16025_16025_B1JdF_pCAGGS_R1_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCNA TTGNAATGAA AATAAATTTC CTTTATTAGC CAGAAGTCAG
   61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG
  121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC
  181 CTGAGGAGTG AATTCGCGCG GTACCAGTGT ACAACCTTCG CCGAAGCTAG AAGCTTCTAG
  241 AGACGCGCTT AGGAGGCACA TCTCGCGCAG CCGCACTTCA CCACTTTCTC AACCTCGTCC
  301 ACAAATGAGG AGCCATCTGT GCATTCGAAA GAGTATTTCC GCCTCTTGCT TCTCAGAGGT
  361 CCACAGCACT GGCCTCCAGC GCACCCGCCT CTGCATTCCA AGCGAGATAC TTTCTTAGTT
  421 GTTTGACAGG CAGCGTAACC CTGCTGCTTC TGGTAATAGT CCCTTATCCG TTCCCCTCGA
  481 CAAGAAATTT CTCTATCACA GCTGTCCCCG GTGAATCCAC TGTTGCATTC ACAATAGGGC
  541 TGGCCCACTC CAGAAAGCCT GCACTTCCCA TGCTTGCACT TGATCATCTG GCANGGGTTA
  601 AAGAGATCTT CTTCTTCATC ACAGAGGACA CCGCCATGGN CCTCCNGGCA CTTGCNACTA
  661 TAGGAGAANG NNTTGATGGG CNGGNNNNNC CNNNGNNNNC NTTTGTTTNC NANGNNGNNN
721 CNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Okada, T., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064. [link to RRC of NBRP]