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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-DEST51-mPeg10-RF1-V5

Catalog number RDB16009
Resource name pEF-DEST51-mPeg10-RF1-V5
Clone info. Gateway(R) expression vector of major tranlation product RF1 of the mouse paternally expressed gene 10 (PEG10) tagged with V5 at C-terminus, EF-1 alpha promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-DEST51 (Plasmid)
Size of vector backbone 7.5 kb
Selectable markers Amp^r (E. coli), Blasticidin (mammalian cells).
Gene/insert name Mouse Peg10 cDNA
Depositor|Developer Maeda, Shingo |
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Mouse Peg10
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1), 13494, 2017). The handling of the property rights for research results obtained by utilizing these resources shall be separately agreed upon by both user of the BIOLOGICAL RESOURCE and the DEPOSITOR.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci. Rep. 7(1), 13494, 2017)。本件リソース利用の結果得られた成果に係る権利の取扱いについては、利用者および寄託者が別途協議するものとする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16009 pEF-DEST51-mPeg10-RF1-V5 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Shinohara, N., TGF-beta signalling and PEG10 are mutually exclusive and inhibitory in chondrosarcoma cells. Sci. Rep. 7 (1): 13494 (2017). PMID 29044189.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RGB16009A8Hep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RGB16009A8Hea.seq check
          1
          Primer: BGH_rev2
          Sequence file: RGB16009A8Heb.seq check
          1
          Primer: SV40pro_f
          Sequence file: RGB16009A8Hec.seq check
          1

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Shinohara, N., TGF-beta signalling and PEG10 are mutually exclusive and inhibitory in chondrosarcoma cells. Sci. Rep. 7 (1): 13494 (2017). PMID 29044189.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl