Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-DEST51-mPeg10-RF1-V5 (#RDB16009)

Clone info. Gateway(R) expression vector of major tranlation product RF1 of the mouse paternally expressed gene 10 (PEG10) tagged with V5 at C-terminus, EF-1 alpha promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-DEST51 (Plasmid)
Size of vector backbone 7.5 kb
Selectable markers Amp^r (E. coli), Blasticidin (mammalian cells).
Gene/insert name Mouse Peg10 cDNA
Depositor|Developer Maeda, Shingo |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1), 13494, 2017). The handling of the property rights for research results obtained by utilizing these resources shall be separately agreed upon by both user of the BIOLOGICAL RESOURCE and the DEPOSITOR.
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci. Rep. 7(1), 13494, 2017)。本件リソース利用の結果得られた成果に係る権利の取扱いについては、利用者および寄託者が別途協議するものとする。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16009 pEF-DEST51-mPeg10-RF1-V5 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-DEST51-mPeg10-RF1-V5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16009).

Reference section:

Shinohara, N., Maeda, S., Yahiro, Y., Sakuma, D., Matsuyama, K., Imamura, K., Kawamura, I., Setoguchi, T., Ishidou, Y., Nagano, S., Komiya, S., TGF-beta signalling and PEG10 are mutually exclusive and inhibitory in chondrosarcoma cells. Sci. Rep. 7 (1): 13494 (2017). PMID 29044189. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RGB16009A8Hep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RGB16009A8Hea.seq check
1
Primer: BGH_rev2
Sequence file: RGB16009A8Heb.seq check
1
Primer: SV40pro_f
Sequence file: RGB16009A8Hec.seq check
1

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shinohara, N., TGF-beta signalling and PEG10 are mutually exclusive and inhibitory in chondrosarcoma cells. Sci. Rep. 7 (1): 13494 (2017). PMID 29044189. [link to RRC of NBRP]