Clone info. | Gateway(R) expression vector of major tranlation product RF1 of the mouse paternally expressed gene 10 (PEG10) tagged with V5 at C-terminus, EF-1 alpha promoter. |
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Comment | Expression was confirmed by the depositor with immunoblotting. |
Vector backbone | pEF-DEST51 (Plasmid) |
Size of vector backbone | 7.5 kb |
Selectable markers | Amp^r (E. coli), Blasticidin (mammalian cells). |
Gene/insert name | Mouse Peg10 cDNA |
Depositor|Developer | Maeda, Shingo | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1), 13494, 2017). The handling of the property rights for research results obtained by utilizing these resources shall be separately agreed upon by both user of the BIOLOGICAL RESOURCE and the DEPOSITOR. |
Remarks | Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci. Rep. 7(1), 13494, 2017)。本件リソース利用の結果得られた成果に係る権利の取扱いについては、利用者および寄託者が別途協議するものとする。 |
備考 | Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16009 | pEF-DEST51-mPeg10-RF1-V5 | DNA solution |
Materials & Methods section:
The pEF-DEST51-mPeg10-RF1-V5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16009). |
Reference section:
Shinohara, N., Maeda, S., Yahiro, Y., Sakuma, D., Matsuyama, K., Imamura, K., Kawamura, I., Setoguchi, T., Ishidou, Y., Nagano, S., Komiya, S., TGF-beta signalling and PEG10 are mutually exclusive and inhibitory in chondrosarcoma cells. Sci. Rep. 7 (1): 13494 (2017). PMID 29044189. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RGB16009A8Hep1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F Sequence file: RGB16009A8Hea.seq ![]() |
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1 |
Primer: BGH_rev2 Sequence file: RGB16009A8Heb.seq ![]() |
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Primer: SV40pro_f Sequence file: RGB16009A8Hec.seq ![]() |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Shinohara, N., TGF-beta signalling and PEG10 are mutually exclusive and inhibitory in chondrosarcoma cells. Sci. Rep. 7 (1): 13494 (2017). PMID 29044189. [link to RRC of NBRP] |
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