Resource data sheet
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pEF-DEST51-mPeg10-RF1-V5

Catalog number RDB16009
Resource name pEF-DEST51-mPeg10-RF1-V5
Clone info. Gateway(R) expression vector of major tranlation product RF1 of the mouse paternally expressed gene 10 (PEG10) tagged with V5 at C-terminus, EF-1 alpha promoter.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-DEST51 (Plasmid)
Size of vector backbone 7.5 kb
Selectable markers Amp^r (E. coli), Blasticidin (mammalian cells).
Gene/insert name Mouse Peg10 cDNA
Depositor Maeda, Shingo |
 
Other clones in our bank mouse Peg10 (NCBI Gene 170676) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1), 13494, 2017). The handling of the property rights for research results obtained by utilizing these resources shall be separately agreed upon by both user of the BIOLOGICAL RESOURCE and the DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16009 pEF-DEST51-mPeg10-RF1-V5 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Shinohara, N., TGF-beta signalling and PEG10 are mutually exclusive and inhibitory in chondrosarcoma cells. Sci. Rep. 7 (1): 13494 (2017). PMID 29044189.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RGB16009A8Hep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RGB16009A8Hea.seq
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Primer: BGH_rev2 RGB16009A8Heb.seq
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Primer: SV40pro_f RGB16009A8Hec.seq
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Please visit Sequencing and PCR primers for primer information.


References

References

original Shinohara, N., TGF-beta signalling and PEG10 are mutually exclusive and inhibitory in chondrosarcoma cells. Sci. Rep. 7 (1): 13494 (2017). PMID 29044189.

2019.02.18

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