Expression vector of sgRNA for left-target of mouse Dmd with hSpCas9-Cdt1(mouse) fusion protein.
Clone info. | Expression vector of sgRNA for left-target of mouse Dmd (5'GGGAAACAAACTACTCATTG3') with hSpCas9-Cdt1(mouse) fusion protein. |
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Vector backbone | pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid) |
Size of vector backbone | 8.5 kb |
Selectable markers | Ampicillin |
Gene/insert name | mouse Cdt1 cDNA mouse Dmd genomic S. pyogenes Cas9 3xFLAG,SV40NLS,nucleospolin NLS |
Depositor|Developer | Sugiyama, Fumihiro | Mizuno, Seiya | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mizuno-Iijima, S. et al., Methods 191: 23-31 (2021)). |
Remarks | The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizuno-Iijima, S. et al., Methods 191: 23-31 (2021))。 |
備考 | 本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB16006 | px330-mC-Dmd-L | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The px330-mC-Dmd-L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16006). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
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