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pET-LnBiT

Bacterial expression vector of LnBiT, a comporntnt of the Open Sandwich Bioluminescent Immunoassay (OS-BLIA)

Catalog number RDB16003
Resource name pET-LnBiT
Clone info. Bacterial expression vector of LnBiT, a comporntnt of the Open Sandwich Bioluminescent Immunoassay (OS-BLIA), for reconstituted Nanoluc-based ternary protein-fragment complementation assay (PCA) system. For constitution of the assay system, please refer Fig.2 of Ohmuro-Matsuyama and Ueda, Anal. Chem., 90 (5): 3001-3004, 2018. Codons are optimized for E. coli expression.
Expression was confirmed by the depositor with open sandwich immunoassay, SDS-PAGE.
Vector backbone pET32b (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Oplophorus gracilorostris Luciferase cDNA
Depositor Ueda, Hiroshi | Ohmuro, Yuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Anal. Chem. 90(5): 3001-3004, 2018). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB16003 pET-LnBiT Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Ohmuro-Matsuyama, Y., Homogeneous Noncompetitive Luminescent Immunodetection of Small Molecules by Ternary Protein Fragment Complementation. Anal. Chem. 90 (5): 3001-3004 (2018). PMID 29446920.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

Original, user report and related articles

original Ohmuro-Matsuyama, Y., Homogeneous Noncompetitive Luminescent Immunodetection of Small Molecules by Ternary Protein Fragment Complementation. Anal. Chem. 90 (5): 3001-3004 (2018). PMID 29446920.

2019.07.17

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