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pET-LnBiT

Bacterial expression vector of LnBiT, a comporntnt of the Open Sandwich Bioluminescent Immunoassay (OS-BLIA)

Catalog number RDB16003
Resource name pET-LnBiT
Clone info. Bacterial expression vector of LnBiT, a comporntnt of the Open Sandwich Bioluminescent Immunoassay (OS-BLIA), for reconstituted Nanoluc-based ternary protein-fragment complementation assay (PCA) system. For constitution of the assay system, please refer Fig.2 of Ohmuro-Matsuyama and Ueda, Anal. Chem., 90 (5): 3001-3004, 2018. Codons are optimized for E. coli expression.
Comment Expression was confirmed by the depositor with open sandwich immunoassay, SDS-PAGE.
Vector backbone pET32b (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Oplophorus gracilorostris Luciferase cDNA
Depositor|Developer Ueda, Hiroshi | Ohmuro, Yuki |
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External Database
Oplophorus gracilorostris Luciferase
            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Anal. Chem. 90(5): 3001-3004, 2018). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Anal. Chem. 90(5): 3001-3004, 2018)。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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            RDB16003 pET-LnBiT Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Ohmuro-Matsuyama, Y., Homogeneous Noncompetitive Luminescent Immunodetection of Small Molecules by Ternary Protein Fragment Complementation. Anal. Chem. 90 (5): 3001-3004 (2018). PMID 29446920.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Ohmuro-Matsuyama, Y., Homogeneous Noncompetitive Luminescent Immunodetection of Small Molecules by Ternary Protein Fragment Complementation. Anal. Chem. 90 (5): 3001-3004 (2018). PMID 29446920.
            review Ohmuro-Matsuyama, Y., Protein-Protein Interaction Assays Using Split-NanoLuc. Bioluminescence (ed. H. Suzuki) (IntechOpen, London) (2019).

            2022.05.17

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