Destabilized CFP (dsCFP) expression vector with CMV promoter.
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Clone info. | Destabilized CFP (dsCFP) expression vector with CMV promoter. PESTsequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017. |
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Comment | The pdsCFP was constructed by replacing the EGFP cassette of pdsGFP with the ECFP sequence of pECFP-1 using the NheI/BspEI. Expression was confirmed by the depositor with Western blotting. |
Vector backbone | pEGFP-C1 (Plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r |
Growth conditions | LB+Kan, 37oC |
Gene/insert name | Mouse Odc1 cDNA |
Depositor|Developer | Mikoshiba, Katsuhiko | Ando, Hideaki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1): 12642, 2017). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci. Rep. 7(1): 12642, 2017)。 |
Catalog # | Resource name | Shipping form | Fee |
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RDB15993 | pdsCFP | DNA solution |
Materials & Methods section:
The pdsCFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15993). |
Reference section:
Ando, H., Hirose, M., Kurosawa, G., Impey, S., Mikoshiba, K., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. [PubMed] [Article] [RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. [PubMed] [Article] [RRC of NBRP] |
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