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pdsCFP (#RDB15993)

Destabilized CFP (dsCFP) expression vector with CMV promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Ando, H., Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Destabilized CFP (dsCFP) expression vector with CMV promoter. PESTsequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017.
Comment The pdsCFP was constructed by replacing the EGFP cassette of pdsGFP with the ECFP sequence of pECFP-1 using the NheI/BspEI. Expression was confirmed by the depositor with Western blotting.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Growth conditions LB+Kan, 37oC
Gene/insert name Mouse Odc1 cDNA
Depositor|Developer Mikoshiba, Katsuhiko | Ando, Hideaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1): 12642, 2017).
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必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci. Rep. 7(1): 12642, 2017)。

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RDB15993 pdsCFP DNA solution

How to cite this biological resource

Materials & Methods section:

The pdsCFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15993).

Reference section:

Ando, H., Hirose, M., Kurosawa, G., Impey, S., Mikoshiba, K., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. [PubMed] [Article] [RRC of NBRP]