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pdsCFP

Destabilized CFP (dsCFP) expression vector with CMV promoter.

Catalog number RDB15993
Resource name pdsCFP
Clone info. Destabilized CFP (dsCFP) expression vector with CMV promoter. PESTsequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017.
Comment The pdsCFP was constructed by replacing the EGFP cassette of pdsGFP with the ECFP sequence of pECFP-1 using the NheI/BspEI. Expression was confirmed by the depositor with Western blotting.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Mouse Odc1 cDNA
Depositor|Developer Mikoshiba, Katsuhiko | Ando, Hideaki |
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External Database
Mouse Odc1

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1): 12642, 2017).
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            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci. Rep. 7(1): 12642, 2017)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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            RDB15993 pdsCFP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15993_A8G9p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV-Forward
            Sequence file: RDB15993_A8G9a.seq check
            >D05582A2-4_A8G9_CMV-Forward_B02_05_ABI24.ab1
                1 CTTCACTCGG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
               61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCCACCA TGGTGAGCAA GGGCGAGGAG
              121 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG
              181 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC
              241 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG
              301 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC
              361 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC
              421 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG
              481 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACATC
              541 AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG
              601 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC
              661 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC
              721 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC
              781 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TAGCCATGGC
              841 TTCCCGCCGG AGGTGGAGGA GCAGGATGAT GGCACGCTGC CCATGTCTTG TGCCCAGGAG
              901 AGCGGGATGG ACCGTCACCC TGCAGCCTGT GCTTCTGCTA GGATCAATGT GTAGAAGCTT
              961 CGAATTCTGC AGTCGACGGT ACCGCGGGCC CGGGATCCAC CGGATCTAGA TAACTGATCA
             1021 TAATCAGCCA TACCACATTT TGTAGAGGAT TTTTACCAGG CCTTTTAAAA AAATCCCTTC
            1081 CTCACAACCC ATTCTGT
            //
            Primer: ME-1290RV
            Sequence file: RDB15993_A8G9b.seq check
            >D05582A2-4_A8G9_ME-1290RV_C02_08_ABI24.ab1
                1 ATCAATTCAT TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
               61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC
              121 GGGCCCGCGG TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG
              181 CACAGGCTGC AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC
              241 CATCATCCTG CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT
              301 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT
              361 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT
              421 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT
              481 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT
              541 GCTTGTCGGC GGTGATATAG ACGTTGTGGC TGATGTAGTT GTACTCCAGC TTGTGCCCCA
              601 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT
              661 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG
              721 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT
              781 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CCCAGGTCAG GGTGGTCACG AGGGTGGGCC
              841 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG
              901 CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT
              961 CGACCAGGAT GGGCACCAAC CCCCGGTGAA ACAGCTCCCT CGCCCTTTGC TCACCAATGG
             1021 GTGACTAGCG GAATCTGACG GGCTTCAACT AAAACCAGCC TCTGCTTTAA TAATAGAACT
            1081 TCCCACCGGT TAACACGCCT ACCGCCCAAT TTTGGCCGTT CA
            //
            Primer: SV40pro_F
            Sequence file: RDB15993_A8G9c.seq check
            >D05582A2-4_A8G9_SV40pro_F_D02_11_ABI24.ab1
                1 CCTTGGGCGT TGGCATTCAG GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
               61 AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
              121 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
              181 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
              241 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG
              301 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
              361 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
              421 CCTGCCGAGA AAGTATCCAT CATGGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
              481 GGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
              541 TGGAAGCCGG TCTTGTCCAA TCAGGATGAT CTGGGACAAA GAGCATCAGG GCTCGCGCCA
              601 GCCCAAACGG TTCGCCAGGC TCAAGGCAAC CTGCCCGACG GCCGAAGATC TCGTCTGAAC
              661 CCATGGCGAT GCCCGGCTTG CCGAATATCT GGTGGGAAAA ATGGGCGGTT TTCTGGGATC
              721 ACCAACGGGG CCGGCGGGGG GGGCGCGAAC CCTTTTCGGA AAACGGTGGC TTCCCGGGAT
              781 ATTTGCAAAA ATTTGGGGCG AAAGGGCTAA CCTTTCTCTG GCTTTTCGGA TTCCCGCGCT
              841 CCCATATGCA GAGCATCGGC TTTTTATAGG CGCTCTGTGA GAGATTCCTC TCAGAAGGCG
              901 GACTCTCTGT GGGTCTCGAA TAGGACCACA CAAGGAGACG CCAACTGCGC TCTCCAGAGG
              961 ATATTTATAA TCGCCGCCGC GTCTATTTAG TAGAGAGTGT GTCTGAGGAG TATACTTTCT
            1021 GAGAACGGCG TTGAATATCA ATCACCCGGA GCATCACA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl