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pdsCFP

Destabilized CFP (dsCFP) expression vector with CMV promoter.

Catalog number RDB15993
Resource name pdsCFP
Clone info. Destabilized CFP (dsCFP) expression vector with CMV promoter. PESTsequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017.
The pdsCFP was constructed by replacing the EGFP cassette of pdsGFP with the ECFP sequence of pECFP-1 using the NheI/BspEI. Expression was confirmed by the depositor with Western blotting.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Mouse Odc1 cDNA
Depositor Mikoshiba, Katsuhiko | Ando, Hideaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1): 12642, 2017).
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RDB15993 pdsCFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

References

original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15993_A8G9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB15993_A8G9a.seq
>D05582A2-4_A8G9_CMV-Forward_B02_05_ABI24.ab1
    1 CTTCACTCGG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCCACCA TGGTGAGCAA GGGCGAGGAG
  121 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG
  181 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC
  241 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG
  301 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC
  361 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC
  421 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG
  481 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACATC
  541 AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG
  601 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC
  661 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC
  721 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC
  781 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TAGCCATGGC
  841 TTCCCGCCGG AGGTGGAGGA GCAGGATGAT GGCACGCTGC CCATGTCTTG TGCCCAGGAG
  901 AGCGGGATGG ACCGTCACCC TGCAGCCTGT GCTTCTGCTA GGATCAATGT GTAGAAGCTT
  961 CGAATTCTGC AGTCGACGGT ACCGCGGGCC CGGGATCCAC CGGATCTAGA TAACTGATCA
 1021 TAATCAGCCA TACCACATTT TGTAGAGGAT TTTTACCAGG CCTTTTAAAA AAATCCCTTC
1081 CTCACAACCC ATTCTGT
//
Primer: ME-1290RV RDB15993_A8G9b.seq
>D05582A2-4_A8G9_ME-1290RV_C02_08_ABI24.ab1
    1 ATCAATTCAT TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
   61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC
  121 GGGCCCGCGG TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG
  181 CACAGGCTGC AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC
  241 CATCATCCTG CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT
  301 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT
  361 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT
  421 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT
  481 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT
  541 GCTTGTCGGC GGTGATATAG ACGTTGTGGC TGATGTAGTT GTACTCCAGC TTGTGCCCCA
  601 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT
  661 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG
  721 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT
  781 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CCCAGGTCAG GGTGGTCACG AGGGTGGGCC
  841 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG
  901 CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT
  961 CGACCAGGAT GGGCACCAAC CCCCGGTGAA ACAGCTCCCT CGCCCTTTGC TCACCAATGG
 1021 GTGACTAGCG GAATCTGACG GGCTTCAACT AAAACCAGCC TCTGCTTTAA TAATAGAACT
1081 TCCCACCGGT TAACACGCCT ACCGCCCAAT TTTGGCCGTT CA
//
Primer: SV40pro_F RDB15993_A8G9c.seq
>D05582A2-4_A8G9_SV40pro_F_D02_11_ABI24.ab1
    1 CCTTGGGCGT TGGCATTCAG GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
  121 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
  181 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
  241 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG
  301 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
  361 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
  421 CCTGCCGAGA AAGTATCCAT CATGGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
  481 GGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
  541 TGGAAGCCGG TCTTGTCCAA TCAGGATGAT CTGGGACAAA GAGCATCAGG GCTCGCGCCA
  601 GCCCAAACGG TTCGCCAGGC TCAAGGCAAC CTGCCCGACG GCCGAAGATC TCGTCTGAAC
  661 CCATGGCGAT GCCCGGCTTG CCGAATATCT GGTGGGAAAA ATGGGCGGTT TTCTGGGATC
  721 ACCAACGGGG CCGGCGGGGG GGGCGCGAAC CCTTTTCGGA AAACGGTGGC TTCCCGGGAT
  781 ATTTGCAAAA ATTTGGGGCG AAAGGGCTAA CCTTTCTCTG GCTTTTCGGA TTCCCGCGCT
  841 CCCATATGCA GAGCATCGGC TTTTTATAGG CGCTCTGTGA GAGATTCCTC TCAGAAGGCG
  901 GACTCTCTGT GGGTCTCGAA TAGGACCACA CAAGGAGACG CCAACTGCGC TCTCCAGAGG
  961 ATATTTATAA TCGCCGCCGC GTCTATTTAG TAGAGAGTGT GTCTGAGGAG TATACTTTCT
1021 GAGAACGGCG TTGAATATCA ATCACCCGGA GCATCACA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

2019.02.26

GNP_filter3_RDBDEP_html_190131.pl