Sequencing and PCR primers for primer information.
Destabilized CFP (dsCFP) expression vector with CMV promoter.
| Catalog number | RDB15993 |
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| Resource name | pdsCFP |
| Clone info. | Destabilized CFP (dsCFP) expression vector with CMV promoter. PESTsequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017. The pdsCFP was constructed by replacing the EGFP cassette of pdsGFP with the ECFP sequence of pECFP-1 using the NheI/BspEI. Expression was confirmed by the depositor with Western blotting. |
| Vector backbone | pEGFP-C1 (Plasmid) |
| Size of vector backbone | 4.7 kb |
| Selectable markers | Kan^r |
| Gene/insert name | Mouse Odc1 cDNA |
| Depositor | Mikoshiba, Katsuhiko | Ando, Hideaki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1): 12642, 2017). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15993 | pdsCFP | DNA solution |
Electronic file
Featured content
| Featured content | Fluorescent protein resources (English text) |
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| Featured content | Fluorescent protein resources (Japanese text) |
References
| original | Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15993_A8G9p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB15993_A8G9a.seq |
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>D05582A2-4_A8G9_CMV-Forward_B02_05_ABI24.ab1 1 CTTCACTCGG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCCACCA TGGTGAGCAA GGGCGAGGAG 121 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG 181 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 241 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG 301 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC 361 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC 421 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG 481 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACATC 541 AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG 601 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC 661 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC 721 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 781 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TAGCCATGGC 841 TTCCCGCCGG AGGTGGAGGA GCAGGATGAT GGCACGCTGC CCATGTCTTG TGCCCAGGAG 901 AGCGGGATGG ACCGTCACCC TGCAGCCTGT GCTTCTGCTA GGATCAATGT GTAGAAGCTT 961 CGAATTCTGC AGTCGACGGT ACCGCGGGCC CGGGATCCAC CGGATCTAGA TAACTGATCA 1021 TAATCAGCCA TACCACATTT TGTAGAGGAT TTTTACCAGG CCTTTTAAAA AAATCCCTTC 1081 CTCACAACCC ATTCTGT // |
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| Primer: ME-1290RV | RDB15993_A8G9b.seq |
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>D05582A2-4_A8G9_ME-1290RV_C02_08_ABI24.ab1 1 ATCAATTCAT TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT 61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC 121 GGGCCCGCGG TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG 181 CACAGGCTGC AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC 241 CATCATCCTG CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT 301 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT 361 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT 421 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT 481 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT 541 GCTTGTCGGC GGTGATATAG ACGTTGTGGC TGATGTAGTT GTACTCCAGC TTGTGCCCCA 601 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT 661 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG 721 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT 781 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CCCAGGTCAG GGTGGTCACG AGGGTGGGCC 841 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG 901 CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT 961 CGACCAGGAT GGGCACCAAC CCCCGGTGAA ACAGCTCCCT CGCCCTTTGC TCACCAATGG 1021 GTGACTAGCG GAATCTGACG GGCTTCAACT AAAACCAGCC TCTGCTTTAA TAATAGAACT 1081 TCCCACCGGT TAACACGCCT ACCGCCCAAT TTTGGCCGTT CA // |
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| Primer: SV40pro_F | RDB15993_A8G9c.seq |
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>D05582A2-4_A8G9_SV40pro_F_D02_11_ABI24.ab1 1 CCTTGGGCGT TGGCATTCAG GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 61 AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 121 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 181 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 241 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG 301 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 361 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 421 CCTGCCGAGA AAGTATCCAT CATGGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC 481 GGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 541 TGGAAGCCGG TCTTGTCCAA TCAGGATGAT CTGGGACAAA GAGCATCAGG GCTCGCGCCA 601 GCCCAAACGG TTCGCCAGGC TCAAGGCAAC CTGCCCGACG GCCGAAGATC TCGTCTGAAC 661 CCATGGCGAT GCCCGGCTTG CCGAATATCT GGTGGGAAAA ATGGGCGGTT TTCTGGGATC 721 ACCAACGGGG CCGGCGGGGG GGGCGCGAAC CCTTTTCGGA AAACGGTGGC TTCCCGGGAT 781 ATTTGCAAAA ATTTGGGGCG AAAGGGCTAA CCTTTCTCTG GCTTTTCGGA TTCCCGCGCT 841 CCCATATGCA GAGCATCGGC TTTTTATAGG CGCTCTGTGA GAGATTCCTC TCAGAAGGCG 901 GACTCTCTGT GGGTCTCGAA TAGGACCACA CAAGGAGACG CCAACTGCGC TCTCCAGAGG 961 ATATTTATAA TCGCCGCCGC GTCTATTTAG TAGAGAGTGT GTCTGAGGAG TATACTTTCT 1021 GAGAACGGCG TTGAATATCA ATCACCCGGA GCATCACA // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. |
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2019.02.26
GNP_filter3_RDBDEP_html_190131.pl