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pdsGFP

Destabilized GFP (dsGFP) expression vector with CMV promoter.

Catalog number RDB15992
Resource name pdsGFP
Clone info. Destabilized GFP (dsGFP) expression vector with CMV promoter. PEST sequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 1 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017.
The pdsGFP was constructed by inserting the PEST sequence of mouse ornithine decarboxylase (422–461aa), with a stop codon, into the BglII/HindIII of pEGFP-C1. Expression was confirmed by the depositor with Western blotting.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Mouse Odc1 cDNA
Depositor Mikoshiba, Katsuhiko | Ando, Hideaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1): 12642, 2017).
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RDB15992 pdsGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

References

original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15992_A8G9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB15992_A8G9a.seq
>D05582A1-1_A8G9_CMV-Forward_A01_01_ABI24.ab1
    1 CTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA
  841 TCTAGCCATG GCTTCCCGCC GGAGGTGGAG GAGCAGGATG ATGGCACGCT GCCCATGTCT
  901 TGTGCCCAGG AGAGCGGGAT GGACCGTCAC CCTGCAGCCT GTGCTTCTGC TAGGATCAAT
  961 GTGTAGAAGC TTCGAAATTC TGCAGTCGAC GGTACCGCGG GGCCCCGGGA TCCCACCCGG
 1021 ATCTAGATAA CTGAATCATT AATCAGCCCA ATAACCAACA TTTGGTTAGA AGGTTTTTAA
1081 CTTGCTTTTA AAAAAAACCT TCCCCCACCA
//
Primer: ME-1290RV RDB15992_A8G9b.seq
>D05582A1-1_A8G9_ME-1290RV_B01_04_ABI24.ab1
    1 ATTCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
   61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC
  121 GGGCCCGCGG TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG
  181 CACAGGCTGC AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC
  241 CATCATCCTG CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT
  301 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT
  361 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT
  421 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT
  481 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT
  541 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA
  601 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT
  661 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG
  721 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT
  781 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC
  841 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAAACTTCA GGGTCAGCTT GCCGTAGGTG
  901 GCAATCGCCC TCGCCCTCGC CGGACACGCT GAAACTTGTG GCCGTTTACG TCGCCGTCCA
  961 GCTCGACCAG GATGGGCACC ACCCCCGGGT GAACAGCTCC TCGCCCTTGC TCACCATTGG
 1021 GTGGCGACCC GGTAGCGCTA GCGGATCCTG GACGGGTTCA CTAAACCCAG CTCTGGCATT
1081 ATAATAGACC TCCCAACCGT TACACGCCTA
//
Primer: SV40pro_F RDB15992_A8G9c.seq
>D05582A1-1_A8G9_SV40pro_F_C01_07_ABI24.ab1
    1 CCGGGGCGTA ACAATTTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
  841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
  901 GGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA
  961 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT TCCGGGACGC CGGCTTGGAT
 1021 GAATCCCTCC CAGCGGCGGG ATCTCAATGG CTGCAGGTTC TTTCGCCCAC CCTTAAGGGG
1081 AGCCTTAACC TTGAAAAACA CGGGAAAAGG GAGGACACCA AGGTAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

2019.02.26

GNP_filter3_RDBDEP_html_190131.pl