Sequencing and PCR primers for primer information.
Destabilized GFP (dsGFP) expression vector with CMV promoter.
| Catalog number | RDB15992 |
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| Resource name | pdsGFP |
| Clone info. | Destabilized GFP (dsGFP) expression vector with CMV promoter. PEST sequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 1 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017. The pdsGFP was constructed by inserting the PEST sequence of mouse ornithine decarboxylase (422–461aa), with a stop codon, into the BglII/HindIII of pEGFP-C1. Expression was confirmed by the depositor with Western blotting. |
| Vector backbone | pEGFP-C1 (Plasmid) |
| Size of vector backbone | 4.7 kb |
| Selectable markers | Kan^r |
| Gene/insert name | Mouse Odc1 cDNA |
| Depositor | Mikoshiba, Katsuhiko | Ando, Hideaki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1): 12642, 2017). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15992 | pdsGFP | DNA solution |
Electronic file
Featured content
| Featured content | Fluorescent protein resources (English text) |
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| Featured content | Fluorescent protein resources (Japanese text) |
References
| original | Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15992_A8G9p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB15992_A8G9a.seq |
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>D05582A1-1_A8G9_CMV-Forward_A01_01_ABI24.ab1 1 CTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC 121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA 181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG 241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC 301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC 361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC 421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC 481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG 541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG 601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC 661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC 721 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG 781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA 841 TCTAGCCATG GCTTCCCGCC GGAGGTGGAG GAGCAGGATG ATGGCACGCT GCCCATGTCT 901 TGTGCCCAGG AGAGCGGGAT GGACCGTCAC CCTGCAGCCT GTGCTTCTGC TAGGATCAAT 961 GTGTAGAAGC TTCGAAATTC TGCAGTCGAC GGTACCGCGG GGCCCCGGGA TCCCACCCGG 1021 ATCTAGATAA CTGAATCATT AATCAGCCCA ATAACCAACA TTTGGTTAGA AGGTTTTTAA 1081 CTTGCTTTTA AAAAAAACCT TCCCCCACCA // |
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| Primer: ME-1290RV | RDB15992_A8G9b.seq |
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>D05582A1-1_A8G9_ME-1290RV_B01_04_ABI24.ab1 1 ATTCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT 61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC 121 GGGCCCGCGG TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG 181 CACAGGCTGC AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC 241 CATCATCCTG CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT 301 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT 361 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT 421 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT 481 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT 541 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA 601 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT 661 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG 721 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT 781 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC 841 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAAACTTCA GGGTCAGCTT GCCGTAGGTG 901 GCAATCGCCC TCGCCCTCGC CGGACACGCT GAAACTTGTG GCCGTTTACG TCGCCGTCCA 961 GCTCGACCAG GATGGGCACC ACCCCCGGGT GAACAGCTCC TCGCCCTTGC TCACCATTGG 1021 GTGGCGACCC GGTAGCGCTA GCGGATCCTG GACGGGTTCA CTAAACCCAG CTCTGGCATT 1081 ATAATAGACC TCCCAACCGT TACACGCCTA // |
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| Primer: SV40pro_F | RDB15992_A8G9c.seq |
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>D05582A1-1_A8G9_SV40pro_F_C01_07_ABI24.ab1 1 CCGGGGCGTA ACAATTTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG CGGCTATCGT 301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 601 AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 901 GGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 961 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT TCCGGGACGC CGGCTTGGAT 1021 GAATCCCTCC CAGCGGCGGG ATCTCAATGG CTGCAGGTTC TTTCGCCCAC CCTTAAGGGG 1081 AGCCTTAACC TTGAAAAACA CGGGAAAAGG GAGGACACCA AGGTAC // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. |
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2019.02.26
GNP_filter3_RDBDEP_html_190131.pl