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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pdsGFP

Destabilized GFP (dsGFP) expression vector with CMV promoter.

Catalog number RDB15992
Resource name pdsGFP
Clone info. Destabilized GFP (dsGFP) expression vector with CMV promoter. PEST sequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 1 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017.
Comment The pdsGFP was constructed by inserting the PEST sequence of mouse ornithine decarboxylase (422–461aa), with a stop codon, into the BglII/HindIII of pEGFP-C1. Expression was confirmed by the depositor with Western blotting.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Mouse Odc1 cDNA
Depositor|Developer Mikoshiba, Katsuhiko | Ando, Hideaki |
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External Database
Mouse Odc1

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sci. Rep. 7(1): 12642, 2017).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci. Rep. 7(1): 12642, 2017)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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            RDB15992 pdsGFP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15992_A8G9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,EGFP 5'
            Sequence file: RDB15992_A8G9c.seq check
            >15992_15992_B1JiF_CMV-Forward_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
               61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
              121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
              181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
              241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
              301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
              361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
              421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
              481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
              541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCN
              601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGANGGCAG CGTGCAGCTC GCCNANCNCT
              661 ACCAGCANAA CACCCCCNTC GGNGANNNNC CCGNGCTGCT GNCCGANNNN CACTNNNNGA
            721 NNNCCNNNTC NNNCNNNNNN NNANNNNNNN NNN
            //
            Primer: SV40polyA-R
            Region: insert (PEST),EGFP 3'
            Sequence file: RDB15992_A8G9d.seq check
            >15992_15992_B1JiF_SV40polyA_R_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNTNNN ACCTCTACNA ATGTGGTATG GCNNNNNNNN
               61 ATCAGTTATC TNNNNNNGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC AGAATTCGAA
              121 GCTTCTACAC ATTGATCCTA GCAGAAGCAC AGGCTGCAGG GTGACGGTCC ATCCCGCTCT
              181 CCTGGGCACA AGACATGGGC AGCGTGCCAT CATCCTGCTC CTCCACCTCC GGCGGGAAGC
              241 CATGGCTAGA TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
              301 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
              361 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
              421 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT
              481 TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT
              541 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA NGTTGCCGTC CTCCTTGAAG TCGATGCCCT
              601 TCAGCTCGAT GCGGTTCACC NGGGTGTCGC CCTCNAACTT CACCTCNGCG CGGGTCTTGT
              661 AGTTGCCGTC GTCCTTNAAN AANNNGGTGC GCTNCNNGNN GTNNCCTTCG GGCATNNNNN
            721 ANTNNNANAN NNNNTGCTGN NTNNNNNNNG NNNNGGGNNN NNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40pro_ori,NeoR_KanR
            Sequence file: RDB15992_A8G9f.seq check
            >15992_15992_B1JiF_SV40polyA_R_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNTNNN ACCTCTACNA ATGTGGTATG GCNNNNNNNN
               61 ATCAGTTATC TNNNNNNGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC AGAATTCGAA
              121 GCTTCTACAC ATTGATCCTA GCAGAAGCAC AGGCTGCAGG GTGACGGTCC ATCCCGCTCT
              181 CCTGGGCACA AGACATGGGC AGCGTGCCAT CATCCTGCTC CTCCACCTCC GGCGGGAAGC
              241 CATGGCTAGA TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
              301 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
              361 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
              421 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT
              481 TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT
              541 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA NGTTGCCGTC CTCCTTGAAG TCGATGCCCT
              601 TCAGCTCGAT GCGGTTCACC NGGGTGTCGC CCTCNAACTT CACCTCNGCG CGGGTCTTGT
              661 AGTTGCCGTC GTCCTTNAAN AANNNGGTGC GCTNCNNGNN GTNNCCTTCG GGCATNNNNN
            721 ANTNNNANAN NNNNTGCTGN NTNNNNNNNG NNNNGGGNNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl