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AGP1UAS-CYC1pro-VTC1ORF

PCR template to create yeast strain of AGP1(UAS)-CYC1(pro)-VTC1(ORF).

Catalog number RDB15991
Resource name AGP1UAS-CYC1pro-VTC1ORF
Alternative name AGP1(UAS)-CYC1(pro)-VTC1(ORF)
Clone info. Plasmid clone as a PCR template to create yeast strain of AGP1UAS-CYC1pro-VTC1ORF.
Comment Expression was confirmed by the depositor with Northern blot. There are three substitutions in CYC1, TATA1:TATATATA to TACGCGTA, TATA3:TAAATATT to TAGGCCTT, TATA4:TTATACATT to TTGCGCATT.
Vector backbone pBluescript II KS(+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name S. cerevisiae VTC1 genomic DNA S. cerevisiae CYC1 genomic DNA S. cerevisiae AGP1 genomic DNA
Depositor|Developer Kokubo, Tetsuro |
Other clones in our bank
External Database
S. cerevisiae VTC1 S. cerevisiae CYC1 S. cerevisiae AGP1
      Reference sequence
        

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (PLoS One, 12 (11), e0188435, 2017).
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      提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (PLoS One, 12(11), e0188435, 2017)。
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      RDB15991 AGP1UAS-CYC1pro-VTC1ORF Under QC test. Please contact us. DNA solution

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      References and tips

      Electronic file

      Original reference

      original Watanabe, K., SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae. PLoS One 12 (11): e0188435 (2017). PMID 29176831.

      Further references such as user reports and related articles (go to bottom)

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      Sequence information

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      References

      Original, user report and related articles

      original Watanabe, K., SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae. PLoS One 12 (11): e0188435 (2017). PMID 29176831.

      2022.05.17

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