Resource data sheet
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AGP1UAS-CYC1pro-VTC1ORF (#RDB15991)

PCR template to create yeast strain of AGP1(UAS)-CYC1(pro)-VTC1(ORF).

Alternative name AGP1(UAS)-CYC1(pro)-VTC1(ORF)
Clone info. Plasmid clone as a PCR template to create yeast strain of AGP1UAS-CYC1pro-VTC1ORF.
Comment Expression was confirmed by the depositor with Northern blot. There are three substitutions in CYC1, TATA1:TATATATA to TACGCGTA, TATA3:TAAATATT to TAGGCCTT, TATA4:TTATACATT to TTGCGCATT.
Vector backbone pBluescript II KS(+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name S. cerevisiae VTC1 genomic DNA S. cerevisiae CYC1 genomic DNA S. cerevisiae AGP1 genomic DNA
Depositor|Developer Kokubo, Tetsuro |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (PLoS One, 12 (11), e0188435, 2017).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (PLoS One, 12(11), e0188435, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15991 AGP1UAS-CYC1pro-VTC1ORF Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The AGP1UAS-CYC1pro-VTC1ORF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15991).

Reference section:

Watanabe, K., Kokubo, T., SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae. PLoS One 12 (11): e0188435 (2017). PMID 29176831. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Watanabe, K., SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae. PLoS One 12 (11): e0188435 (2017). PMID 29176831. [link to RRC of NBRP]