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AGP1UAS-CYC1pro-VTC1ORF

PCR template to create yeast strain of AGP1(UAS)-CYC1(pro)-VTC1(ORF).

Catalog number RDB15991
Resource name AGP1UAS-CYC1pro-VTC1ORF
Alternative name AGP1(UAS)-CYC1(pro)-VTC1(ORF)
Clone info. Plasmid clone as a PCR template to create yeast strain of AGP1UAS-CYC1pro-VTC1ORF.
Expression was confirmed by the depositor with Northern blot. There are three substitutions in CYC1, TATA1:TATATATA to TACGCGTA, TATA3:TAAATATT to TAGGCCTT, TATA4:TTATACATT to TTGCGCATT.
Vector backbone pBluescript II KS(+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name S. cerevisiae VTC1 genomic DNA S. cerevisiae CYC1 genomic DNA S. cerevisiae AGP1 genomic DNA
Depositor Kokubo, Tetsuro |
 
Other clones in our bank S. cerevisiae VTC1 | S. cerevisiae CYC1 (KEGG orthology K08738) | S. cerevisiae AGP1 (KEGG orthology K16261) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (PLoS One, 12 (11), e0188435, 2017).
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RDB15991 AGP1UAS-CYC1pro-VTC1ORF Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Watanabe, K., SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae. PLoS One 12 (11): e0188435 (2017). PMID 29176831.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Watanabe, K., SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae. PLoS One 12 (11): e0188435 (2017). PMID 29176831.

2019.07.17

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