Bifidobacterium longum–E. coli shuttle vector.
Clone info. | Bifidobacterium longum–E. coli shuttle vector. |
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Comment | The SpR gene confers spectinomycin resistance both in E. coli and Bifidobacterium longum 105-A. The depositor uses spectinomycin at a concentration of 75 microgram/mL for selection of transformants in agar media. B. longum 105-A (JCM 31944) is available from JCM as a host strain. |
Vector backbone | pBS423 (Plasmid) |
Size of vector backbone | 4.9 kb |
Selectable markers | SpR |
Reference of insert sequence | AB687999 (DDBJ accession number) |
Depositor|Developer | Fukiya, Satoru | Kano, Yasunobu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは,非営利の学術研究に限って提供する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15974 | pBS423 | DNA solution |
Materials & Methods section:
The pBS423 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15974). |
Reference section:
Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15974_A8Fbp1-4.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pBS423_seq3_4 (Pr1260) Region: insert mid(repA 5') Sequence file: RDB15974_A8Fbg.seq |
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>15974_15974_B2LdF_M2_pBS423_seq3_4_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NCGNNNCTTC GCTGGGCTGA NCCATCTGNC TTGGTTCGGC 61 GCACGCTTGT GCAATGCACA AATGTGCAAT AGGATGTGAG CTATGAGCAA CGAATATGTG 121 CAGGGGACGC TGGAGCTGAC GCGGGCGTTC GACGGCTGGT GGCTTCCCCA GCGTCCCCTG 181 TGCTGCGACG ATGACTACTC CCAGCTGGTG CGCCGGAGCC GCATCGACGC GCTCAGATGC 241 AAGCACATCG AGGCGAATCC GGCAGCGCAG GTGAACATGC TCGTTGTTGA CGTTGATTCG 301 TCTGAGGGCC GTTTAATGAG CCTGTGGGGG CATCGGGACA TGCCGCCCAA CTTCATCGCG 361 GAGAATCCGG CCAACGGCCA CTGCCACGCG GGCTGGGTGC TCACCGAACC CGTCTGCCGC 421 ACCGACATGG CCAGACTCAA GCCCCTGAAG CTCCTGCACG CCGTCACCGA GGGGCTGCGC 481 CGCAGCGTGG ACGGCGACGA GGGCTATTCC GGCCTGCTCA TGAAGAACCC GTTGAGCGAC 541 GCGTGGGACA GCGACCTGTG CCGCGAGGAC ACCTACGACC TGCCCGACCT CGTGGCCGCG 601 CTCGAAGCGC ACGGGGACAT GCCGCCCAAG AGCTGGACGC GCACCAAACG CGCCCGCGAG 661 GTCGGAGTGG GCCGCAACTG CACCCTGTTC GACGAGGCCC GCACCCTCGC CTACCGCGAG 721 GTGCGCCGAC TGCCTGACCG CACGCCCGCC TCCTCCGACC TGCTGCGCGA GTACGTGCGC 781 CGCACCTGCC ACGAAATCAA CGCCTCGTTC CCCGACCCGC TGCCCGTGCG CGAGGTCAAC 841 GACACCGCCN AGAGCATCCA CAAGTGGATC ACCACNNNAA AGCCGCATGT GGCGANGACG 901 GTGCCGTCNC CNANCNNCCG CCNNCNTTCG TCNNCNNNCC AATNCCGNNN NNNCNGNAAA 961 GAAANNNNGG GNGNNGNCCA GANNAAAANN NNNNTTTNNA NNNNNNNNTT CGCGNNNNTN 1021 ANNNNGCNNN GGANNGNNGC NTNNGNANNN TNGNNCNNNN NNNNNN // |
Primer: pTBR101CM_seq3 (Pr0560) Region: insert mid(pTB4_p1 3') Sequence file: RDB15974_A8Fbh.seq |
>D04829A3-12_A8Fb_pTBR101CM_seq3_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTGG NNGNTCCGCC GNNCNTGGGG CATGGGTCTT 61 CCCGGCAAGC CGATTCGCAC CGCGAATCGA GTGATTGTAG CTACAATCAC GTCGCTTGCT 121 ACTCCGGCCT TCGGCACTCG GGTTGTTGCT CCTGCGCCGA AGGGCGAGGG AGGCTGCGGG 181 CTCGGTTCAT GTACATGAAC CGTCTAGCTT GCTTACCTTC GATTTGATGG ACATCTCCAT 241 GTGGATGTCC ATGGAGATGT CCATGGAGAT GTCCATGGAG ATGTCCATGG AGATGTCCAT 301 GGAGATGTCC ATGTGGGTGT CCATGTGGGT GTCCATGGAT ATATCCATCT GAGCGCATAG 361 GAAAACGCAT GANGNNGGAT GATCGTTCGG GGTGATGCCT TCTTCCACGA ACGGGAAGGG 421 GCTCTTCCTT TTTCTGGAAT GTCATCTTCC AGCGCCCTTC GCCTTGAGCT CCTTGAGCAG 481 CATCGTCGCG TTGTCGCGGA TGCTCGCGTC CGTGAGCGGG GAGAGCTCGT CCGCATAGGA 541 GCGGCAGAGG AGCGCGTACA CCCGTTCCCG CACCCAGGCG CTGCGCGCGA TGCCCAGCTT 601 GAACGCGGCC CGGCACAGCA TCTCGTATTC GTCGCCGGTG AGCAGGACAT TGAGCTGGCG 661 TCAAAAGTTG ATTAGTCATG CCGTGTATAT ACGTCCGGGG TGTTTTCGGC CAGATTCCAA 721 CAGTTTTGGG CCACTCCGGG AAGGAACAGC CGACGCTTCG CTGGGCTGAA CCATCTGACT 781 TGGTTCGGCG CACGCTTGTG CAATGCACAA ATGTGCAATA GGATGTGAGC TATGANCAAC 841 GAATATGTGC ANGGGGANGC TGGANCTGAC GCNGGNGTTC GACGGNNGGN GGCTTCCCCA 901 GCGNCCCCNG NGCNGCNANG ATGACTACTC CNANCNGGNG NNNCNNNNCC GCATCGNCGC 961 GCTCNNATGN NNGCACATCN NGGCGANNNC NNNNNNCGCA NNNNNANNNN NNNNNN // |
Primer: ColE1ori_F (Pr0393) Region: ori,insert(pTB4_p3,pTB4_p2) Sequence file: RDB15974_A8Fbi.seq |
>D04829A3-12_A8Fb_ColE1ori_F_H01_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNC NNCCTCTGNN TTGNNCGTCG ATTTTTGTGA NGCTCGTCAG 61 GGGGGCGGAN CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTCC CTGGCCTTTT 121 GCTGGCCTTT TGCTCAGAAT TCGAGCTCGG TACCCGGGGN TCCAGGTGCT TGACAGGGTG 181 ATGGAGCGTC ACCCAGAACT GTCCGAGAAG GACGTGGTGA CCGCGTTCCG CAGCGTCATG 241 GTGGACGCGG AGCGCGAGTC CGGCGCGTGG ATGGCGATAG GGCTCGACGG GCGCGGCAGG 301 AACGTCGAGA TGCTGTACAG GGTCGTCGGC GACCTCGTGG TGATCTACCA CGCGTTCACG 361 CCGCCGACCA AGAAGTTCAG GCGCGAGATA GACCGTCTGC GCGGCGATGG GAGGACGCTA 421 TGAGTGATTT CAGCGAACGC GACAGGCAGT TGATGGCGCT GGTCGGGCTG ACCGAGGAGC 481 AGGTGCTGGA GGATGAGCGG ATGGCCGAGT CCGAGACTGT CCCGGACGAC CTGACGGGGC 541 GCGTGTACTA CGGGCTCCAT CTCGCCGAGC CCGACGAGCA GATGGTCAGC GTGTCCGTCC 601 GCATGCCCAA GAGCACGCTG GACGGTCTGA CCGCGATGGC CAGGCGGTAC CACATCAGCC 661 GCAGCGAGTA CATGCGCCGT AAGCTCGCTG GAGCGATCTG AGACCTGTCA GGCCGATGGT 721 TCGAATCACG TAACACCCGC GCACCACCGT GAGGATGTGC GGGTGTTACG TGATGTGCCA 781 CAGTCGTGGC ACGATGCCGC GTGANCGGTC TGGNNGNNTC TGCCGACTAT GGGGCATGGG 841 TCTTNCCNGC AAGCCGATTC NNACCGNNNA ATCGANNGNA NCGTATATNN NGTTCACGTC 901 NCTTGCNACN NCNNGNNCNT NGCNGNCTTT TNNCTCNNNN NNCNNNNTNN NNTNGNNGNC 961 NNNGGTANGG NNCGNTNGGC NNNNNNNNNN // |
Primer: pBS423_seq3_5 (Pr1261) Region: insert mid(repB 5',repA 3') Sequence file: RDB15974_A8Fbj.seq |
>15974_15974_B2LdF_M2_pBS423_seq3_5_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC GTTNCTTGCG GGCGCGGTAN GCGCGTTGGC 61 GCACGGCACC TTGGGTCATG TTGAAGTGTT CGGCGGTCTG GCTCCACGAG TGGCCGTCAT 121 CGTCGTGGTA GGCGCGGATG GATTCGCGCA ACGTTGCCTG CTCGTCTATC CAGTCCTCGC 181 GCGTTTGCGA ATTCCAGTAC TTGATCGTGC GCACGGACAC CCCATAGGCC TCGGCTAACT 241 CCTTGGCGGG CAATGGGCGC TTCTTGCGAA TTGTCTGGAT GGTCATTGTC CAAGCACCTC 301 CTGCGCGTAC TGCGCGAACT TCTCTTCAAA TGCGTTTTGT CTGGCCTCGC CCGATTTCTT 361 TCCGCGTGCG GATTGGATGG CGACGAACGT GGCGGCGTTG GCGACGGCAC CGTCTCGCCA 421 CATGCGGCTT CGCGTGGTGA TCCACTTGTG GATGCTCTTG GCGGTGTCGT TGACCTCGCG 481 CACGGGCAGC GGGTCGGGGA ACGAGGCGTT GATTTCGTGG CAGGTGCGGC GCACGTACTC 541 GCGCAGCAGG TCGGAGGAGG CGGGCGTGCG GTCAGGCAGT CGGCGCACCT CGCGGTAGGC 601 GAGGGTGCGG GCCTCGTCGA ACAGGGTGCA GTTGCGGCCC ACTCCGACCT CGCGGGCGCG 661 TTTGGTGCGC GTCCAGCTCT TGGGCGGCAT GTCCCCGTGC GCTTCGAGCG CGGCCACGAN 721 GTCGGGCAGG TCGTANGTGT CCTCNCGGCN NANGTCGCTG TNNCACNCNN CNNTNNNNGG 781 GNNTNCTTCA TGANCAGGCC GGAATAGCCC TCGNCNCNNN NNNNNNCTNN CNGNNGCANN 841 NCCNNNCNGG TGANNGCNTG CNNNNNCTTC NNNGGGCTTN NANNNNGNNN NNNNNCNGTG 901 NNGNNNNAAN NGGTTTCNGG TGANCNNNNC ANNCCNNNNG NNANNNNGNN NNTNGGCCNN 961 NNNNNNNCNN NNNNNNNN // |
Primer: pTBR101CM_seq7 (Pr0561) Region: insert mid(repB, 3',pTB4_p2,pTB4_p3 3') Sequence file: RDB15974_A8Fbk.seq |
>D04829A3-12_A8Fb_pTBR101CM_seq7_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNTCCGC GCCNANNNCG ACNATGNNGG CCACTCGTGG ANCCAGACCG 61 CCGAACACTT CAACATGACC CAAGGTGCCG TGCGCCAACG CGCCTACCGC GCCCGCAAGG 121 AACGCGAGGC CGAGGCAAAA GCCGCAAGGC CGGAGTAGCA AGTGACGTGA ACGTATATAC 181 GTTCACTCGA TTCGCGGTGC GAATCGGCTT GCCGGGAAGA CCCATGCCCC ATAGTCGGCA 241 GAGCCTCCAG ACCGCTCACG CGGCATCGTG CCACGACTGT GGCACATCAC GTAACACCCG 301 CACATCCTCA CGGTGGTGCG CGGGTGTTAC GTGATTCGAA CCATCGGCCT GACAGGTCTC 361 AGATCGCTCC AGCGAGCTTA CGGCGCATGT ACTCGCTGCG GCTGATGTGG TACCGCCTGG 421 CCATCGCGGT CAGACCGTCC AGCGTGCTCT TGGGCATGCG GACGGACACG CTGACCATCT 481 GCTCGTCGGG CTCGGCGAGA TGGAGCCCGT AGTACACGCG CCCCGTCAGG TCGTCCGGGA 541 CAGTCTCGGA CTCGGCCATC CGCTCATCCT CCAGCACCTG CTCCTCGGTC AGCCCGACCA 601 GCGCCATCAA CTGCCTGTCG CGTTCGCTGA AATCACTCAT AGCGTCCTCC CATCGCCGCG 661 CAGACGGTCT ATCTCGCGCC TGAACTTCTT GGTCGGCGGC GTGAACGCGT GGTAGATCAC 721 CACGAGGTCG CCGACGACCC TGTACAGCAT CTCGACGTTC CTGCCGCGCC CGTCGAGCCC 781 TATCGCCATC CACGCGCCGG ANTCGCGCTC CGCGTCCACC ATGACGCTGC GGAACGCGGT 841 CACCACGTCC TTCTCNGANA GTTCTGGGTG ANGCTCCATC ACCCNGNCAA GCACCTGGNT 901 NCCCGGGNAC CGANCTCNAA TTCNGANCAA AANGNNNGCA AAAGGNNNNG GNNNNTAAAA 961 NNNNCNNGTN GCNNGNNNNT TTTNCNNNNN NNCNNNCCCC NNNNNNANCN NNNNNAANNN 1021 NNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. [link to RRC of NBRP] |
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reference | Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016). |
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