Sequencing and PCR primers for primer information.
Bifidobacterium longum–E. coli shuttle vector.
| Catalog number | RDB15974 |
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| Resource name | pBS423 |
| Clone info. | Bifidobacterium longum–E. coli shuttle vector. The SpR gene confers spectinomycin resistance both in E. coli and Bifidobacterium longum 105-A. The depositor uses spectinomycin at a concentration of 75 microgram/mL for selection of transformants in agar media. B. longum 105-A (JCM 31944) is available from JCM as a host strain. |
| Vector backbone | pBS423 (Plasmid) |
| Size of vector backbone | 4.9 kb |
| Selectable markers | SpR |
| Reference of nucleotide sequence | AB687999 (DDBJ accession number) |
| Depositor | Fukiya, Satoru | Kano, Yasunobu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15974 | pBS423 | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Bacteria host strains (en) (English text) |
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| Featured content | Bacteria host strains (ja) (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15974_A8Fbp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pBS423_seq3 | Sequence: RDB15974_A8Fba.seq |
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>D04829A3-12_A8Fb_pBS423_seq3_E01_05_ABI08.ab1 1 GCCGCGCAAG TGACATGCTC GTTGTTGACG TTGATTCGTC TGAGGGCCGT TTAATGAGCC 61 TGTGGGGGCA TCGGGACATG CCGCCCAACT TCATCGCGGA GAATCCGGCC AACGGCCACT 121 GCCACGCGGG CTGGGTGCTC ACCGAACCCG TCTGCCGCAC CGACATGGCC AGACTCAAGC 181 CCCTGAAGCT CCTGCACGCC GTCACCGAGG GGCTGCGCCG CAGCGTGGAC GGCGACGAGG 241 GCTATTCCGG CCTGCTCATG AAGAACCCGT TGAGCGACGC GTGGGACAGC GACCTGTGCC 301 GCGAGGACAC CTACGACCTG CCCGACCTCG TGGCCGCGCT CGAAGCGCAC GGGGACATGC 361 CGCCCAAGAG CTGGACGCGC ACCAAACGCG CCCGCGAGGT CGGAGTGGGC CGCAACTGCA 421 CCCTGTTCGA CGAGGCCCGC ACCCTCGCCT ACCGCGAGGT GCGCCGACTG CCTGACCGCA 481 CGCCCGCCTC CTCCGACCTG CTGCGCGAGT ACGTGCGCCG CACCTGCCAC GAAATCAACG 541 CCTCGTTCCC CGACCCGCTG CCCGTGCGCG AGGTCAACGA CACCGCCAAG AGCATCCACA 601 AGTGGATCAC CACGCGAAGC CGCATGTGGC GAGACGGTGC CGTCGCCAAC GCCGCCACGT 661 TCGTCGCCAT CCAATCCGCA CGCGGAAAGA AATCGGGCGA GGCCAGACAA AACGCATTTG 721 AAGAGAAGTT CGCGCAGTAC GCGCAGGAGG TGCTTGGACA ATGACCATCC AGACAATTCG 781 CAAGAAGCGC CCATTGCCCG CCAAGGAGTT AGCCGAGGCC TATGGGGGTG TCCGTGCGCA 841 CGATCAAGTA CTGGAATTCG CAAACGCGCG AGGACTGGAT AGACGAGCAG GCAACGTTGC 901 GCGAATCCAT CCGCGCCTAC CACGACGATG ACGGCCACTC GTGGAGCCAG ACCGCCGAAC 961 ACTTCAACAT GACCCAAAGG TGCCGTGCGC CCAACGCGCC CTACCGCGCC CGCAAAGGAA 1021 ACGCGAGGCC GAGGCAAAAG CCGCAAAGGC CCGGGAGTAG CAGTGACGTG AACGTATAAT 1081 ACGATCAACT TCGAATCGCG GCTGCCGAAA TCGGCCTTGT CGGGAAGGAA T // |
| Primer: pTBR101CM_seq7 | Sequence: RDB15974_A8Fbb.seq |
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>D04829A3-12_A8Fb_pTBR101CM_seq7_G01_07_ABI08.ab1 1 CAATTGCGCG GATCATCCGC GCCTACCACG ACGATGACGG CCACTCGTGG AGCCAGACCG 61 CCGAACACTT CAACATGACC CAAGGTGCCG TGCGCCAACG CGCCTACCGC GCCCGCAAGG 121 AACGCGAGGC CGAGGCAAAA GCCGCAAGGC CGGAGTAGCA AGTGACGTGA ACGTATATAC 181 GTTCACTCGA TTCGCGGTGC GAATCGGCTT GCCGGGAAGA CCCATGCCCC ATAGTCGGCA 241 GAGCCTCCAG ACCGCTCACG CGGCATCGTG CCACGACTGT GGCACATCAC GTAACACCCG 301 CACATCCTCA CGGTGGTGCG CGGGTGTTAC GTGATTCGAA CCATCGGCCT GACAGGTCTC 361 AGATCGCTCC AGCGAGCTTA CGGCGCATGT ACTCGCTGCG GCTGATGTGG TACCGCCTGG 421 CCATCGCGGT CAGACCGTCC AGCGTGCTCT TGGGCATGCG GACGGACACG CTGACCATCT 481 GCTCGTCGGG CTCGGCGAGA TGGAGCCCGT AGTACACGCG CCCCGTCAGG TCGTCCGGGA 541 CAGTCTCGGA CTCGGCCATC CGCTCATCCT CCAGCACCTG CTCCTCGGTC AGCCCGACCA 601 GCGCCATCAA CTGCCTGTCG CGTTCGCTGA AATCACTCAT AGCGTCCTCC CATCGCCGCG 661 CAGACGGTCT ATCTCGCGCC TGAACTTCTT GGTCGGCGGC GTGAACGCGT GGTAGATCAC 721 CACGAGGTCG CCGACGACCC TGTACAGCAT CTCGACGTTC CTGCCGCGCC CGTCGAGCCC 781 TATCGCCATC CACGCGCCGG ACTCGCGCTC CGCGTCCACC ATGACGCTGC GGAACGCGGT 841 CACCACGTCC TTCTCGGACA GTTCTGGGTG ACGCTCCATC ACCCTGTCAA GCACCTGGAT 901 CCCCGGGTAC CGAGCTCGAA TTCTGAGCAA AAGGCCAGCA AAAGGCCAGG GACCGTAAAA 961 AGGCCGCGTT GCTGGGCGTT TTTCCATAGC TCCCGCCCCC CTGACGAGCA TCACAAAATC 1021 GACGCTCAAG GTCAGAGTGC GAAACCCGAC AGGAACTATA AGGATACCAA GGGCGGTTTC 1081 CCCCTTGGAG GCTCCTTCGT TGCGCTCCTC TTGGTCGAAA CCCTGGTCCG CCTA // |
| Primer: pTBR101CM_seq3 | Sequence: RDB15974_A8Fbc.seq |
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>D04829A3-12_A8Fb_pTBR101CM_seq3_F01_06_ABI08.ab1 1 TGTCCGGGAA GCGCCGCGAA ACGGGTCTGG AGGCTCCGCC GACCATGGGG CATGGGTCTT 61 CCCGGCAAGC CGATTCGCAC CGCGAATCGA GTGATTGTAG CTACAATCAC GTCGCTTGCT 121 ACTCCGGCCT TCGGCACTCG GGTTGTTGCT CCTGCGCCGA AGGGCGAGGG AGGCTGCGGG 181 CTCGGTTCAT GTACATGAAC CGTCTAGCTT GCTTACCTTC GATTTGATGG ACATCTCCAT 241 GTGGATGTCC ATGGAGATGT CCATGGAGAT GTCCATGGAG ATGTCCATGG AGATGTCCAT 301 GGAGATGTCC ATGTGGGTGT CCATGTGGGT GTCCATGGAT ATATCCATCT GAGCGCATAG 361 GAAAACGCAT GAGGGTGGAT GATCGTTCGG GGTGATGCCT TCTTCCACGA ACGGGAAGGG 421 GCTCTTCCTT TTTCTGGAAT GTCATCTTCC AGCGCCCTTC GCCTTGAGCT CCTTGAGCAG 481 CATCGTCGCG TTGTCGCGGA TGCTCGCGTC CGTGAGCGGG GAGAGCTCGT CCGCATAGGA 541 GCGGCAGAGG AGCGCGTACA CCCGTTCCCG CACCCAGGCG CTGCGCGCGA TGCCCAGCTT 601 GAACGCGGCC CGGCACAGCA TCTCGTATTC GTCGCCGGTG AGCAGGACAT TGAGCTGGCG 661 TCAAAAGTTG ATTAGTCATG CCGTGTATAT ACGTCCGGGG TGTTTTCGGC CAGATTCCAA 721 CAGTTTTGGG CCACTCCGGG AAGGAACAGC CGACGCTTCG CTGGGCTGAA CCATCTGACT 781 TGGTTCGGCG CACGCTTGTG CAATGCACAA ATGTGCAATA GGATGTGAGC TATGAGCAAC 841 GAATATGTGC AGGGGGACGC TGGAGCTGAC GCGGGCGTTC GACGGCTGGT GGCTTCCCCA 901 GCGTCCCCTG TGCTGCGACG ATGACTACTC CCAGCTGGTG CGCCGGAGCC GCATCGACGC 961 GCTCAGATGC AAGCACATCG AGGCGAATCC CGGCAGCGCA GGTGAAACAT GCTCGTTGTT 1021 GACGTTTGAA TTTCGTCTGA GGACCCGTTT AATGAGCCTG GTTGGGGGGC ATCGGGACAT 1081 GGCCGGCCGA ACTTCAATCG GCGAGAATTC GGCCAACGGA CCACCGTGTC CAACGGC // |
| Primer: ColE1ori_F | Sequence: RDB15974_A8Fbd.seq |
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>D04829A3-12_A8Fb_ColE1ori_F_H01_08_ABI08.ab1 1 CCCCATGTCT GTCGGATCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 61 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTCC CTGGCCTTTT 121 GCTGGCCTTT TGCTCAGAAT TCGAGCTCGG TACCCGGGGA TCCAGGTGCT TGACAGGGTG 181 ATGGAGCGTC ACCCAGAACT GTCCGAGAAG GACGTGGTGA CCGCGTTCCG CAGCGTCATG 241 GTGGACGCGG AGCGCGAGTC CGGCGCGTGG ATGGCGATAG GGCTCGACGG GCGCGGCAGG 301 AACGTCGAGA TGCTGTACAG GGTCGTCGGC GACCTCGTGG TGATCTACCA CGCGTTCACG 361 CCGCCGACCA AGAAGTTCAG GCGCGAGATA GACCGTCTGC GCGGCGATGG GAGGACGCTA 421 TGAGTGATTT CAGCGAACGC GACAGGCAGT TGATGGCGCT GGTCGGGCTG ACCGAGGAGC 481 AGGTGCTGGA GGATGAGCGG ATGGCCGAGT CCGAGACTGT CCCGGACGAC CTGACGGGGC 541 GCGTGTACTA CGGGCTCCAT CTCGCCGAGC CCGACGAGCA GATGGTCAGC GTGTCCGTCC 601 GCATGCCCAA GAGCACGCTG GACGGTCTGA CCGCGATGGC CAGGCGGTAC CACATCAGCC 661 GCAGCGAGTA CATGCGCCGT AAGCTCGCTG GAGCGATCTG AGACCTGTCA GGCCGATGGT 721 TCGAATCACG TAACACCCGC GCACCACCGT GAGGATGTGC GGGTGTTACG TGATGTGCCA 781 CAGTCGTGGC ACGATGCCGC GTGAGCGGTC TGGAGGGCTC TGCCGACTAT GGGGCATGGG 841 TCTTCCCGGC AAGCCGATTC GCACCGCGGA ATCGAGTGGA ACGTATATAA CGTTCACGTC 901 ACTTGCTACT TCCGGGCCTT TGCGGGCTTT TGCCTCGGCC CTCGCGTTTC CTTTGCGGGC 961 GCCGGTAGGG CGCGTTTGGC GCACGGCAAC CTTTGGGTTC AATGTTTGAA AGTGGTTCGG 1021 CCGGTTCTTG GTTCCACGAG TGGGCCCGGT CAATCGTCGT TGGTAAGCGC GGATGGGAAT 1081 TTCGCGCAAC GGTTGGCTTG CTCGTCTATC AGGTCCTCGC CGCGATTTGC GCGAAAATCC 1141 GGATTATGCT ATGGGATGTC CGTGT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. |
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| reference | Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016). |
2019.11.02
GNP_filter3_RDBDEP_html_190927.pl