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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS423 (#RDB15974)

Bifidobacterium longum–E. coli shuttle vector.

Clone info. Bifidobacterium longum–E. coli shuttle vector.
Comment The SpR gene confers spectinomycin resistance both in E. coli and Bifidobacterium longum 105-A. The depositor uses spectinomycin at a concentration of 75 microgram/mL for selection of transformants in agar media. B. longum 105-A (JCM 31944) is available from JCM as a host strain.
Vector backbone pBS423 (Plasmid)
Size of vector backbone 4.9 kb
Selectable markers SpR
Reference of insert sequence AB687999 (DDBJ accession number)
Depositor|Developer Fukiya, Satoru | Kano, Yasunobu |
 

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは,非営利の学術研究に限って提供する。

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RDB15974 pBS423 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBS423 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15974).

Reference section:

Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15974_A8Fbp1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBS423_seq3_4 (Pr1260)
Region: insert mid(repA 5')
Sequence file: RDB15974_A8Fbg.seq check
>15974_15974_B2LdF_M2_pBS423_seq3_4_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCGNNNCTTC GCTGGGCTGA NCCATCTGNC TTGGTTCGGC
   61 GCACGCTTGT GCAATGCACA AATGTGCAAT AGGATGTGAG CTATGAGCAA CGAATATGTG
  121 CAGGGGACGC TGGAGCTGAC GCGGGCGTTC GACGGCTGGT GGCTTCCCCA GCGTCCCCTG
  181 TGCTGCGACG ATGACTACTC CCAGCTGGTG CGCCGGAGCC GCATCGACGC GCTCAGATGC
  241 AAGCACATCG AGGCGAATCC GGCAGCGCAG GTGAACATGC TCGTTGTTGA CGTTGATTCG
  301 TCTGAGGGCC GTTTAATGAG CCTGTGGGGG CATCGGGACA TGCCGCCCAA CTTCATCGCG
  361 GAGAATCCGG CCAACGGCCA CTGCCACGCG GGCTGGGTGC TCACCGAACC CGTCTGCCGC
  421 ACCGACATGG CCAGACTCAA GCCCCTGAAG CTCCTGCACG CCGTCACCGA GGGGCTGCGC
  481 CGCAGCGTGG ACGGCGACGA GGGCTATTCC GGCCTGCTCA TGAAGAACCC GTTGAGCGAC
  541 GCGTGGGACA GCGACCTGTG CCGCGAGGAC ACCTACGACC TGCCCGACCT CGTGGCCGCG
  601 CTCGAAGCGC ACGGGGACAT GCCGCCCAAG AGCTGGACGC GCACCAAACG CGCCCGCGAG
  661 GTCGGAGTGG GCCGCAACTG CACCCTGTTC GACGAGGCCC GCACCCTCGC CTACCGCGAG
  721 GTGCGCCGAC TGCCTGACCG CACGCCCGCC TCCTCCGACC TGCTGCGCGA GTACGTGCGC
  781 CGCACCTGCC ACGAAATCAA CGCCTCGTTC CCCGACCCGC TGCCCGTGCG CGAGGTCAAC
  841 GACACCGCCN AGAGCATCCA CAAGTGGATC ACCACNNNAA AGCCGCATGT GGCGANGACG
  901 GTGCCGTCNC CNANCNNCCG CCNNCNTTCG TCNNCNNNCC AATNCCGNNN NNNCNGNAAA
  961 GAAANNNNGG GNGNNGNCCA GANNAAAANN NNNNTTTNNA NNNNNNNNTT CGCGNNNNTN
1021 ANNNNGCNNN GGANNGNNGC NTNNGNANNN TNGNNCNNNN NNNNNN
//
Primer: pTBR101CM_seq3 (Pr0560)
Region: insert mid(pTB4_p1 3')
Sequence file: RDB15974_A8Fbh.seq check
>D04829A3-12_A8Fb_pTBR101CM_seq3_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTGG NNGNTCCGCC GNNCNTGGGG CATGGGTCTT
   61 CCCGGCAAGC CGATTCGCAC CGCGAATCGA GTGATTGTAG CTACAATCAC GTCGCTTGCT
  121 ACTCCGGCCT TCGGCACTCG GGTTGTTGCT CCTGCGCCGA AGGGCGAGGG AGGCTGCGGG
  181 CTCGGTTCAT GTACATGAAC CGTCTAGCTT GCTTACCTTC GATTTGATGG ACATCTCCAT
  241 GTGGATGTCC ATGGAGATGT CCATGGAGAT GTCCATGGAG ATGTCCATGG AGATGTCCAT
  301 GGAGATGTCC ATGTGGGTGT CCATGTGGGT GTCCATGGAT ATATCCATCT GAGCGCATAG
  361 GAAAACGCAT GANGNNGGAT GATCGTTCGG GGTGATGCCT TCTTCCACGA ACGGGAAGGG
  421 GCTCTTCCTT TTTCTGGAAT GTCATCTTCC AGCGCCCTTC GCCTTGAGCT CCTTGAGCAG
  481 CATCGTCGCG TTGTCGCGGA TGCTCGCGTC CGTGAGCGGG GAGAGCTCGT CCGCATAGGA
  541 GCGGCAGAGG AGCGCGTACA CCCGTTCCCG CACCCAGGCG CTGCGCGCGA TGCCCAGCTT
  601 GAACGCGGCC CGGCACAGCA TCTCGTATTC GTCGCCGGTG AGCAGGACAT TGAGCTGGCG
  661 TCAAAAGTTG ATTAGTCATG CCGTGTATAT ACGTCCGGGG TGTTTTCGGC CAGATTCCAA
  721 CAGTTTTGGG CCACTCCGGG AAGGAACAGC CGACGCTTCG CTGGGCTGAA CCATCTGACT
  781 TGGTTCGGCG CACGCTTGTG CAATGCACAA ATGTGCAATA GGATGTGAGC TATGANCAAC
  841 GAATATGTGC ANGGGGANGC TGGANCTGAC GCNGGNGTTC GACGGNNGGN GGCTTCCCCA
  901 GCGNCCCCNG NGCNGCNANG ATGACTACTC CNANCNGGNG NNNCNNNNCC GCATCGNCGC
961 GCTCNNATGN NNGCACATCN NGGCGANNNC NNNNNNCGCA NNNNNANNNN NNNNNN
//
Primer: ColE1ori_F (Pr0393)
Region: ori,insert(pTB4_p3,pTB4_p2)
Sequence file: RDB15974_A8Fbi.seq check
>D04829A3-12_A8Fb_ColE1ori_F_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNCCTCTGNN TTGNNCGTCG ATTTTTGTGA NGCTCGTCAG
   61 GGGGGCGGAN CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTCC CTGGCCTTTT
  121 GCTGGCCTTT TGCTCAGAAT TCGAGCTCGG TACCCGGGGN TCCAGGTGCT TGACAGGGTG
  181 ATGGAGCGTC ACCCAGAACT GTCCGAGAAG GACGTGGTGA CCGCGTTCCG CAGCGTCATG
  241 GTGGACGCGG AGCGCGAGTC CGGCGCGTGG ATGGCGATAG GGCTCGACGG GCGCGGCAGG
  301 AACGTCGAGA TGCTGTACAG GGTCGTCGGC GACCTCGTGG TGATCTACCA CGCGTTCACG
  361 CCGCCGACCA AGAAGTTCAG GCGCGAGATA GACCGTCTGC GCGGCGATGG GAGGACGCTA
  421 TGAGTGATTT CAGCGAACGC GACAGGCAGT TGATGGCGCT GGTCGGGCTG ACCGAGGAGC
  481 AGGTGCTGGA GGATGAGCGG ATGGCCGAGT CCGAGACTGT CCCGGACGAC CTGACGGGGC
  541 GCGTGTACTA CGGGCTCCAT CTCGCCGAGC CCGACGAGCA GATGGTCAGC GTGTCCGTCC
  601 GCATGCCCAA GAGCACGCTG GACGGTCTGA CCGCGATGGC CAGGCGGTAC CACATCAGCC
  661 GCAGCGAGTA CATGCGCCGT AAGCTCGCTG GAGCGATCTG AGACCTGTCA GGCCGATGGT
  721 TCGAATCACG TAACACCCGC GCACCACCGT GAGGATGTGC GGGTGTTACG TGATGTGCCA
  781 CAGTCGTGGC ACGATGCCGC GTGANCGGTC TGGNNGNNTC TGCCGACTAT GGGGCATGGG
  841 TCTTNCCNGC AAGCCGATTC NNACCGNNNA ATCGANNGNA NCGTATATNN NGTTCACGTC
  901 NCTTGCNACN NCNNGNNCNT NGCNGNCTTT TNNCTCNNNN NNCNNNNTNN NNTNGNNGNC
961 NNNGGTANGG NNCGNTNGGC NNNNNNNNNN
//
Primer: pBS423_seq3_5 (Pr1261)
Region: insert mid(repB 5',repA 3')
Sequence file: RDB15974_A8Fbj.seq check
>15974_15974_B2LdF_M2_pBS423_seq3_5_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC GTTNCTTGCG GGCGCGGTAN GCGCGTTGGC
   61 GCACGGCACC TTGGGTCATG TTGAAGTGTT CGGCGGTCTG GCTCCACGAG TGGCCGTCAT
  121 CGTCGTGGTA GGCGCGGATG GATTCGCGCA ACGTTGCCTG CTCGTCTATC CAGTCCTCGC
  181 GCGTTTGCGA ATTCCAGTAC TTGATCGTGC GCACGGACAC CCCATAGGCC TCGGCTAACT
  241 CCTTGGCGGG CAATGGGCGC TTCTTGCGAA TTGTCTGGAT GGTCATTGTC CAAGCACCTC
  301 CTGCGCGTAC TGCGCGAACT TCTCTTCAAA TGCGTTTTGT CTGGCCTCGC CCGATTTCTT
  361 TCCGCGTGCG GATTGGATGG CGACGAACGT GGCGGCGTTG GCGACGGCAC CGTCTCGCCA
  421 CATGCGGCTT CGCGTGGTGA TCCACTTGTG GATGCTCTTG GCGGTGTCGT TGACCTCGCG
  481 CACGGGCAGC GGGTCGGGGA ACGAGGCGTT GATTTCGTGG CAGGTGCGGC GCACGTACTC
  541 GCGCAGCAGG TCGGAGGAGG CGGGCGTGCG GTCAGGCAGT CGGCGCACCT CGCGGTAGGC
  601 GAGGGTGCGG GCCTCGTCGA ACAGGGTGCA GTTGCGGCCC ACTCCGACCT CGCGGGCGCG
  661 TTTGGTGCGC GTCCAGCTCT TGGGCGGCAT GTCCCCGTGC GCTTCGAGCG CGGCCACGAN
  721 GTCGGGCAGG TCGTANGTGT CCTCNCGGCN NANGTCGCTG TNNCACNCNN CNNTNNNNGG
  781 GNNTNCTTCA TGANCAGGCC GGAATAGCCC TCGNCNCNNN NNNNNNCTNN CNGNNGCANN
  841 NCCNNNCNGG TGANNGCNTG CNNNNNCTTC NNNGGGCTTN NANNNNGNNN NNNNNCNGTG
  901 NNGNNNNAAN NGGTTTCNGG TGANCNNNNC ANNCCNNNNG NNANNNNGNN NNTNGGCCNN
961 NNNNNNNCNN NNNNNNNN
//
Primer: pTBR101CM_seq7 (Pr0561)
Region: insert mid(repB, 3',pTB4_p2,pTB4_p3 3')
Sequence file: RDB15974_A8Fbk.seq check
>D04829A3-12_A8Fb_pTBR101CM_seq7_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNTCCGC GCCNANNNCG ACNATGNNGG CCACTCGTGG ANCCAGACCG
   61 CCGAACACTT CAACATGACC CAAGGTGCCG TGCGCCAACG CGCCTACCGC GCCCGCAAGG
  121 AACGCGAGGC CGAGGCAAAA GCCGCAAGGC CGGAGTAGCA AGTGACGTGA ACGTATATAC
  181 GTTCACTCGA TTCGCGGTGC GAATCGGCTT GCCGGGAAGA CCCATGCCCC ATAGTCGGCA
  241 GAGCCTCCAG ACCGCTCACG CGGCATCGTG CCACGACTGT GGCACATCAC GTAACACCCG
  301 CACATCCTCA CGGTGGTGCG CGGGTGTTAC GTGATTCGAA CCATCGGCCT GACAGGTCTC
  361 AGATCGCTCC AGCGAGCTTA CGGCGCATGT ACTCGCTGCG GCTGATGTGG TACCGCCTGG
  421 CCATCGCGGT CAGACCGTCC AGCGTGCTCT TGGGCATGCG GACGGACACG CTGACCATCT
  481 GCTCGTCGGG CTCGGCGAGA TGGAGCCCGT AGTACACGCG CCCCGTCAGG TCGTCCGGGA
  541 CAGTCTCGGA CTCGGCCATC CGCTCATCCT CCAGCACCTG CTCCTCGGTC AGCCCGACCA
  601 GCGCCATCAA CTGCCTGTCG CGTTCGCTGA AATCACTCAT AGCGTCCTCC CATCGCCGCG
  661 CAGACGGTCT ATCTCGCGCC TGAACTTCTT GGTCGGCGGC GTGAACGCGT GGTAGATCAC
  721 CACGAGGTCG CCGACGACCC TGTACAGCAT CTCGACGTTC CTGCCGCGCC CGTCGAGCCC
  781 TATCGCCATC CACGCGCCGG ANTCGCGCTC CGCGTCCACC ATGACGCTGC GGAACGCGGT
  841 CACCACGTCC TTCTCNGANA GTTCTGGGTG ANGCTCCATC ACCCNGNCAA GCACCTGGNT
  901 NCCCGGGNAC CGANCTCNAA TTCNGANCAA AANGNNNGCA AAAGGNNNNG GNNNNTAAAA
  961 NNNNCNNGTN GCNNGNNNNT TTTNCNNNNN NNCNNNCCCC NNNNNNANCN NNNNNAANNN
1021 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. [link to RRC of NBRP]
reference Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016).