Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBS423

Bifidobacterium longum–E. coli shuttle vector.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは，非営利の学術研究に限って提供する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15974_A8Fbp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ColE1ori_F Sequence file: RDB15974_A8Fbd.seq
>D04829A3-12_A8Fb_pBS423_seq3_E01_05_ABI08.ab1     1 GCCGCGCAAG TGACATGCTC GTTGTTGACG TTGATTCGTC TGAGGGCCGT TTAATGAGCC    61 TGTGGGGGCA TCGGGACATG CCGCCCAACT TCATCGCGGA GAATCCGGCC AACGGCCACT   121 GCCACGCGGG CTGGGTGCTC ACCGAACCCG TCTGCCGCAC CGACATGGCC AGACTCAAGC   181 CCCTGAAGCT CCTGCACGCC GTCACCGAGG GGCTGCGCCG CAGCGTGGAC GGCGACGAGG   241 GCTATTCCGG CCTGCTCATG AAGAACCCGT TGAGCGACGC GTGGGACAGC GACCTGTGCC   301 GCGAGGACAC CTACGACCTG CCCGACCTCG TGGCCGCGCT CGAAGCGCAC GGGGACATGC   361 CGCCCAAGAG CTGGACGCGC ACCAAACGCG CCCGCGAGGT CGGAGTGGGC CGCAACTGCA   421 CCCTGTTCGA CGAGGCCCGC ACCCTCGCCT ACCGCGAGGT GCGCCGACTG CCTGACCGCA   481 CGCCCGCCTC CTCCGACCTG CTGCGCGAGT ACGTGCGCCG CACCTGCCAC GAAATCAACG   541 CCTCGTTCCC CGACCCGCTG CCCGTGCGCG AGGTCAACGA CACCGCCAAG AGCATCCACA   601 AGTGGATCAC CACGCGAAGC CGCATGTGGC GAGACGGTGC CGTCGCCAAC GCCGCCACGT   661 TCGTCGCCAT CCAATCCGCA CGCGGAAAGA AATCGGGCGA GGCCAGACAA AACGCATTTG   721 AAGAGAAGTT CGCGCAGTAC GCGCAGGAGG TGCTTGGACA ATGACCATCC AGACAATTCG   781 CAAGAAGCGC CCATTGCCCG CCAAGGAGTT AGCCGAGGCC TATGGGGGTG TCCGTGCGCA   841 CGATCAAGTA CTGGAATTCG CAAACGCGCG AGGACTGGAT AGACGAGCAG GCAACGTTGC   901 GCGAATCCAT CCGCGCCTAC CACGACGATG ACGGCCACTC GTGGAGCCAG ACCGCCGAAC   961 ACTTCAACAT GACCCAAAGG TGCCGTGCGC CCAACGCGCC CTACCGCGCC CGCAAAGGAA  1021 ACGCGAGGCC GAGGCAAAAG CCGCAAAGGC CCGGGAGTAG CAGTGACGTG AACGTATAAT 1081 ACGATCAACT TCGAATCGCG GCTGCCGAAA TCGGCCTTGT CGGGAAGGAA T //
Primer: pTBR101CM_seq7 Region: rep B,pTB4_p2,pTB4_p3 Sequence file: RDB15974_A8Fbe.seq
>D04829A3-12_A8Fb_pTBR101CM_seq3_F01_06_ABI08.ab1     1 TGTCCGGGAA GCGCCGCGAA ACGGGTCTGG AGGCTCCGCC GACCATGGGG CATGGGTCTT    61 CCCGGCAAGC CGATTCGCAC CGCGAATCGA GTGATTGTAG CTACAATCAC GTCGCTTGCT   121 ACTCCGGCCT TCGGCACTCG GGTTGTTGCT CCTGCGCCGA AGGGCGAGGG AGGCTGCGGG   181 CTCGGTTCAT GTACATGAAC CGTCTAGCTT GCTTACCTTC GATTTGATGG ACATCTCCAT   241 GTGGATGTCC ATGGAGATGT CCATGGAGAT GTCCATGGAG ATGTCCATGG AGATGTCCAT   301 GGAGATGTCC ATGTGGGTGT CCATGTGGGT GTCCATGGAT ATATCCATCT GAGCGCATAG   361 GAAAACGCAT GAGGGTGGAT GATCGTTCGG GGTGATGCCT TCTTCCACGA ACGGGAAGGG   421 GCTCTTCCTT TTTCTGGAAT GTCATCTTCC AGCGCCCTTC GCCTTGAGCT CCTTGAGCAG   481 CATCGTCGCG TTGTCGCGGA TGCTCGCGTC CGTGAGCGGG GAGAGCTCGT CCGCATAGGA   541 GCGGCAGAGG AGCGCGTACA CCCGTTCCCG CACCCAGGCG CTGCGCGCGA TGCCCAGCTT   601 GAACGCGGCC CGGCACAGCA TCTCGTATTC GTCGCCGGTG AGCAGGACAT TGAGCTGGCG   661 TCAAAAGTTG ATTAGTCATG CCGTGTATAT ACGTCCGGGG TGTTTTCGGC CAGATTCCAA   721 CAGTTTTGGG CCACTCCGGG AAGGAACAGC CGACGCTTCG CTGGGCTGAA CCATCTGACT   781 TGGTTCGGCG CACGCTTGTG CAATGCACAA ATGTGCAATA GGATGTGAGC TATGAGCAAC   841 GAATATGTGC AGGGGGACGC TGGAGCTGAC GCGGGCGTTC GACGGCTGGT GGCTTCCCCA   901 GCGTCCCCTG TGCTGCGACG ATGACTACTC CCAGCTGGTG CGCCGGAGCC GCATCGACGC   961 GCTCAGATGC AAGCACATCG AGGCGAATCC CGGCAGCGCA GGTGAAACAT GCTCGTTGTT  1021 GACGTTTGAA TTTCGTCTGA GGACCCGTTT AATGAGCCTG GTTGGGGGGC ATCGGGACAT 1081 GGCCGGCCGA ACTTCAATCG GCGAGAATTC GGCCAACGGA CCACCGTGTC CAACGGC //
Primer: pTBR101CM_seq3 Region: pTB4_p1,rep A 5' Sequence file: RDB15974_A8Fbf.seq
>D04829A3-12_A8Fb_pTBR101CM_seq7_G01_07_ABI08.ab1     1 CAATTGCGCG GATCATCCGC GCCTACCACG ACGATGACGG CCACTCGTGG AGCCAGACCG    61 CCGAACACTT CAACATGACC CAAGGTGCCG TGCGCCAACG CGCCTACCGC GCCCGCAAGG   121 AACGCGAGGC CGAGGCAAAA GCCGCAAGGC CGGAGTAGCA AGTGACGTGA ACGTATATAC   181 GTTCACTCGA TTCGCGGTGC GAATCGGCTT GCCGGGAAGA CCCATGCCCC ATAGTCGGCA   241 GAGCCTCCAG ACCGCTCACG CGGCATCGTG CCACGACTGT GGCACATCAC GTAACACCCG   301 CACATCCTCA CGGTGGTGCG CGGGTGTTAC GTGATTCGAA CCATCGGCCT GACAGGTCTC   361 AGATCGCTCC AGCGAGCTTA CGGCGCATGT ACTCGCTGCG GCTGATGTGG TACCGCCTGG   421 CCATCGCGGT CAGACCGTCC AGCGTGCTCT TGGGCATGCG GACGGACACG CTGACCATCT   481 GCTCGTCGGG CTCGGCGAGA TGGAGCCCGT AGTACACGCG CCCCGTCAGG TCGTCCGGGA   541 CAGTCTCGGA CTCGGCCATC CGCTCATCCT CCAGCACCTG CTCCTCGGTC AGCCCGACCA   601 GCGCCATCAA CTGCCTGTCG CGTTCGCTGA AATCACTCAT AGCGTCCTCC CATCGCCGCG   661 CAGACGGTCT ATCTCGCGCC TGAACTTCTT GGTCGGCGGC GTGAACGCGT GGTAGATCAC   721 CACGAGGTCG CCGACGACCC TGTACAGCAT CTCGACGTTC CTGCCGCGCC CGTCGAGCCC   781 TATCGCCATC CACGCGCCGG ACTCGCGCTC CGCGTCCACC ATGACGCTGC GGAACGCGGT   841 CACCACGTCC TTCTCGGACA GTTCTGGGTG ACGCTCCATC ACCCTGTCAA GCACCTGGAT   901 CCCCGGGTAC CGAGCTCGAA TTCTGAGCAA AAGGCCAGCA AAAGGCCAGG GACCGTAAAA   961 AGGCCGCGTT GCTGGGCGTT TTTCCATAGC TCCCGCCCCC CTGACGAGCA TCACAAAATC  1021 GACGCTCAAG GTCAGAGTGC GAAACCCGAC AGGAACTATA AGGATACCAA GGGCGGTTTC 1081 CCCCTTGGAG GCTCCTTCGT TGCGCTCCTC TTGGTCGAAA CCCTGGTCCG CCTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl