Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBS423

Bifidobacterium longum–E. coli shuttle vector.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは，非営利の学術研究に限って提供する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15974_A8Fbp1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBS423_seq3_4 (Pr1260) Region: insert mid(repA 5') Sequence file: RDB15974_A8Fbg.seq
>15974_15974_B2LdF_M2_pBS423_seq3_4_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NCGNNNCTTC GCTGGGCTGA NCCATCTGNC TTGGTTCGGC    61 GCACGCTTGT GCAATGCACA AATGTGCAAT AGGATGTGAG CTATGAGCAA CGAATATGTG   121 CAGGGGACGC TGGAGCTGAC GCGGGCGTTC GACGGCTGGT GGCTTCCCCA GCGTCCCCTG   181 TGCTGCGACG ATGACTACTC CCAGCTGGTG CGCCGGAGCC GCATCGACGC GCTCAGATGC   241 AAGCACATCG AGGCGAATCC GGCAGCGCAG GTGAACATGC TCGTTGTTGA CGTTGATTCG   301 TCTGAGGGCC GTTTAATGAG CCTGTGGGGG CATCGGGACA TGCCGCCCAA CTTCATCGCG   361 GAGAATCCGG CCAACGGCCA CTGCCACGCG GGCTGGGTGC TCACCGAACC CGTCTGCCGC   421 ACCGACATGG CCAGACTCAA GCCCCTGAAG CTCCTGCACG CCGTCACCGA GGGGCTGCGC   481 CGCAGCGTGG ACGGCGACGA GGGCTATTCC GGCCTGCTCA TGAAGAACCC GTTGAGCGAC   541 GCGTGGGACA GCGACCTGTG CCGCGAGGAC ACCTACGACC TGCCCGACCT CGTGGCCGCG   601 CTCGAAGCGC ACGGGGACAT GCCGCCCAAG AGCTGGACGC GCACCAAACG CGCCCGCGAG   661 GTCGGAGTGG GCCGCAACTG CACCCTGTTC GACGAGGCCC GCACCCTCGC CTACCGCGAG   721 GTGCGCCGAC TGCCTGACCG CACGCCCGCC TCCTCCGACC TGCTGCGCGA GTACGTGCGC   781 CGCACCTGCC ACGAAATCAA CGCCTCGTTC CCCGACCCGC TGCCCGTGCG CGAGGTCAAC   841 GACACCGCCN AGAGCATCCA CAAGTGGATC ACCACNNNAA AGCCGCATGT GGCGANGACG   901 GTGCCGTCNC CNANCNNCCG CCNNCNTTCG TCNNCNNNCC AATNCCGNNN NNNCNGNAAA   961 GAAANNNNGG GNGNNGNCCA GANNAAAANN NNNNTTTNNA NNNNNNNNTT CGCGNNNNTN 1021 ANNNNGCNNN GGANNGNNGC NTNNGNANNN TNGNNCNNNN NNNNNN //
Primer: pTBR101CM_seq3 (Pr0560) Region: insert mid(pTB4_p1 3') Sequence file: RDB15974_A8Fbh.seq
>D04829A3-12_A8Fb_pTBR101CM_seq3_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTGG NNGNTCCGCC GNNCNTGGGG CATGGGTCTT    61 CCCGGCAAGC CGATTCGCAC CGCGAATCGA GTGATTGTAG CTACAATCAC GTCGCTTGCT   121 ACTCCGGCCT TCGGCACTCG GGTTGTTGCT CCTGCGCCGA AGGGCGAGGG AGGCTGCGGG   181 CTCGGTTCAT GTACATGAAC CGTCTAGCTT GCTTACCTTC GATTTGATGG ACATCTCCAT   241 GTGGATGTCC ATGGAGATGT CCATGGAGAT GTCCATGGAG ATGTCCATGG AGATGTCCAT   301 GGAGATGTCC ATGTGGGTGT CCATGTGGGT GTCCATGGAT ATATCCATCT GAGCGCATAG   361 GAAAACGCAT GANGNNGGAT GATCGTTCGG GGTGATGCCT TCTTCCACGA ACGGGAAGGG   421 GCTCTTCCTT TTTCTGGAAT GTCATCTTCC AGCGCCCTTC GCCTTGAGCT CCTTGAGCAG   481 CATCGTCGCG TTGTCGCGGA TGCTCGCGTC CGTGAGCGGG GAGAGCTCGT CCGCATAGGA   541 GCGGCAGAGG AGCGCGTACA CCCGTTCCCG CACCCAGGCG CTGCGCGCGA TGCCCAGCTT   601 GAACGCGGCC CGGCACAGCA TCTCGTATTC GTCGCCGGTG AGCAGGACAT TGAGCTGGCG   661 TCAAAAGTTG ATTAGTCATG CCGTGTATAT ACGTCCGGGG TGTTTTCGGC CAGATTCCAA   721 CAGTTTTGGG CCACTCCGGG AAGGAACAGC CGACGCTTCG CTGGGCTGAA CCATCTGACT   781 TGGTTCGGCG CACGCTTGTG CAATGCACAA ATGTGCAATA GGATGTGAGC TATGANCAAC   841 GAATATGTGC ANGGGGANGC TGGANCTGAC GCNGGNGTTC GACGGNNGGN GGCTTCCCCA   901 GCGNCCCCNG NGCNGCNANG ATGACTACTC CNANCNGGNG NNNCNNNNCC GCATCGNCGC 961 GCTCNNATGN NNGCACATCN NGGCGANNNC NNNNNNCGCA NNNNNANNNN NNNNNN //
Primer: ColE1ori_F (Pr0393) Region: ori,insert(pTB4_p3,pTB4_p2) Sequence file: RDB15974_A8Fbi.seq
>D04829A3-12_A8Fb_ColE1ori_F_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNC NNCCTCTGNN TTGNNCGTCG ATTTTTGTGA NGCTCGTCAG    61 GGGGGCGGAN CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTCC CTGGCCTTTT   121 GCTGGCCTTT TGCTCAGAAT TCGAGCTCGG TACCCGGGGN TCCAGGTGCT TGACAGGGTG   181 ATGGAGCGTC ACCCAGAACT GTCCGAGAAG GACGTGGTGA CCGCGTTCCG CAGCGTCATG   241 GTGGACGCGG AGCGCGAGTC CGGCGCGTGG ATGGCGATAG GGCTCGACGG GCGCGGCAGG   301 AACGTCGAGA TGCTGTACAG GGTCGTCGGC GACCTCGTGG TGATCTACCA CGCGTTCACG   361 CCGCCGACCA AGAAGTTCAG GCGCGAGATA GACCGTCTGC GCGGCGATGG GAGGACGCTA   421 TGAGTGATTT CAGCGAACGC GACAGGCAGT TGATGGCGCT GGTCGGGCTG ACCGAGGAGC   481 AGGTGCTGGA GGATGAGCGG ATGGCCGAGT CCGAGACTGT CCCGGACGAC CTGACGGGGC   541 GCGTGTACTA CGGGCTCCAT CTCGCCGAGC CCGACGAGCA GATGGTCAGC GTGTCCGTCC   601 GCATGCCCAA GAGCACGCTG GACGGTCTGA CCGCGATGGC CAGGCGGTAC CACATCAGCC   661 GCAGCGAGTA CATGCGCCGT AAGCTCGCTG GAGCGATCTG AGACCTGTCA GGCCGATGGT   721 TCGAATCACG TAACACCCGC GCACCACCGT GAGGATGTGC GGGTGTTACG TGATGTGCCA   781 CAGTCGTGGC ACGATGCCGC GTGANCGGTC TGGNNGNNTC TGCCGACTAT GGGGCATGGG   841 TCTTNCCNGC AAGCCGATTC NNACCGNNNA ATCGANNGNA NCGTATATNN NGTTCACGTC   901 NCTTGCNACN NCNNGNNCNT NGCNGNCTTT TNNCTCNNNN NNCNNNNTNN NNTNGNNGNC 961 NNNGGTANGG NNCGNTNGGC NNNNNNNNNN //
Primer: pBS423_seq3_5 (Pr1261) Region: insert mid(repB 5',repA 3') Sequence file: RDB15974_A8Fbj.seq
>15974_15974_B2LdF_M2_pBS423_seq3_5_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC GTTNCTTGCG GGCGCGGTAN GCGCGTTGGC    61 GCACGGCACC TTGGGTCATG TTGAAGTGTT CGGCGGTCTG GCTCCACGAG TGGCCGTCAT   121 CGTCGTGGTA GGCGCGGATG GATTCGCGCA ACGTTGCCTG CTCGTCTATC CAGTCCTCGC   181 GCGTTTGCGA ATTCCAGTAC TTGATCGTGC GCACGGACAC CCCATAGGCC TCGGCTAACT   241 CCTTGGCGGG CAATGGGCGC TTCTTGCGAA TTGTCTGGAT GGTCATTGTC CAAGCACCTC   301 CTGCGCGTAC TGCGCGAACT TCTCTTCAAA TGCGTTTTGT CTGGCCTCGC CCGATTTCTT   361 TCCGCGTGCG GATTGGATGG CGACGAACGT GGCGGCGTTG GCGACGGCAC CGTCTCGCCA   421 CATGCGGCTT CGCGTGGTGA TCCACTTGTG GATGCTCTTG GCGGTGTCGT TGACCTCGCG   481 CACGGGCAGC GGGTCGGGGA ACGAGGCGTT GATTTCGTGG CAGGTGCGGC GCACGTACTC   541 GCGCAGCAGG TCGGAGGAGG CGGGCGTGCG GTCAGGCAGT CGGCGCACCT CGCGGTAGGC   601 GAGGGTGCGG GCCTCGTCGA ACAGGGTGCA GTTGCGGCCC ACTCCGACCT CGCGGGCGCG   661 TTTGGTGCGC GTCCAGCTCT TGGGCGGCAT GTCCCCGTGC GCTTCGAGCG CGGCCACGAN   721 GTCGGGCAGG TCGTANGTGT CCTCNCGGCN NANGTCGCTG TNNCACNCNN CNNTNNNNGG   781 GNNTNCTTCA TGANCAGGCC GGAATAGCCC TCGNCNCNNN NNNNNNCTNN CNGNNGCANN   841 NCCNNNCNGG TGANNGCNTG CNNNNNCTTC NNNGGGCTTN NANNNNGNNN NNNNNCNGTG   901 NNGNNNNAAN NGGTTTCNGG TGANCNNNNC ANNCCNNNNG NNANNNNGNN NNTNGGCCNN 961 NNNNNNNCNN NNNNNNNN //
Primer: pTBR101CM_seq7 (Pr0561) Region: insert mid(repB, 3',pTB4_p2,pTB4_p3 3') Sequence file: RDB15974_A8Fbk.seq
>D04829A3-12_A8Fb_pTBR101CM_seq7_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNTCCGC GCCNANNNCG ACNATGNNGG CCACTCGTGG ANCCAGACCG    61 CCGAACACTT CAACATGACC CAAGGTGCCG TGCGCCAACG CGCCTACCGC GCCCGCAAGG   121 AACGCGAGGC CGAGGCAAAA GCCGCAAGGC CGGAGTAGCA AGTGACGTGA ACGTATATAC   181 GTTCACTCGA TTCGCGGTGC GAATCGGCTT GCCGGGAAGA CCCATGCCCC ATAGTCGGCA   241 GAGCCTCCAG ACCGCTCACG CGGCATCGTG CCACGACTGT GGCACATCAC GTAACACCCG   301 CACATCCTCA CGGTGGTGCG CGGGTGTTAC GTGATTCGAA CCATCGGCCT GACAGGTCTC   361 AGATCGCTCC AGCGAGCTTA CGGCGCATGT ACTCGCTGCG GCTGATGTGG TACCGCCTGG   421 CCATCGCGGT CAGACCGTCC AGCGTGCTCT TGGGCATGCG GACGGACACG CTGACCATCT   481 GCTCGTCGGG CTCGGCGAGA TGGAGCCCGT AGTACACGCG CCCCGTCAGG TCGTCCGGGA   541 CAGTCTCGGA CTCGGCCATC CGCTCATCCT CCAGCACCTG CTCCTCGGTC AGCCCGACCA   601 GCGCCATCAA CTGCCTGTCG CGTTCGCTGA AATCACTCAT AGCGTCCTCC CATCGCCGCG   661 CAGACGGTCT ATCTCGCGCC TGAACTTCTT GGTCGGCGGC GTGAACGCGT GGTAGATCAC   721 CACGAGGTCG CCGACGACCC TGTACAGCAT CTCGACGTTC CTGCCGCGCC CGTCGAGCCC   781 TATCGCCATC CACGCGCCGG ANTCGCGCTC CGCGTCCACC ATGACGCTGC GGAACGCGGT   841 CACCACGTCC TTCTCNGANA GTTCTGGGTG ANGCTCCATC ACCCNGNCAA GCACCTGGNT   901 NCCCGGGNAC CGANCTCNAA TTCNGANCAA AANGNNNGCA AAAGGNNNNG GNNNNTAAAA   961 NNNNCNNGTN GCNNGNNNNT TTTNCNNNNN NNCNNNCCCC NNNNNNANCN NNNNNAANNN 1021 NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl