Resource data sheet
Prev.

pBS423

Bifidobacterium longum–E. coli shuttle vector.

Catalog number RDB15974
Resource name pBS423
Clone info. Bifidobacterium longum–E. coli shuttle vector.
The SpR gene confers spectinomycin resistance both in E. coli and Bifidobacterium longum 105-A. The depositor uses spectinomycin at a concentration of 75 microgram/mL for selection of transformants in agar media. B. longum 105-A (JCM 31944) is available from JCM as a host strain.
Vector backbone pBS423 (Plasmid)
Size of vector backbone 4.9 kb
Selectable markers SpR
Reference of nucleotide sequence AB687999 (DDBJ accession number)
Depositor Fukiya, Satoru | Kano, Yasunobu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
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RDB15974 pBS423 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Bacteria host strains (en) (English text)
Featured content Bacteria host strains (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15974_A8Fbp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBS423_seq3 | Sequence: RDB15974_A8Fba.seq
>D04829A3-12_A8Fb_pBS423_seq3_E01_05_ABI08.ab1
    1 GCCGCGCAAG TGACATGCTC GTTGTTGACG TTGATTCGTC TGAGGGCCGT TTAATGAGCC
   61 TGTGGGGGCA TCGGGACATG CCGCCCAACT TCATCGCGGA GAATCCGGCC AACGGCCACT
  121 GCCACGCGGG CTGGGTGCTC ACCGAACCCG TCTGCCGCAC CGACATGGCC AGACTCAAGC
  181 CCCTGAAGCT CCTGCACGCC GTCACCGAGG GGCTGCGCCG CAGCGTGGAC GGCGACGAGG
  241 GCTATTCCGG CCTGCTCATG AAGAACCCGT TGAGCGACGC GTGGGACAGC GACCTGTGCC
  301 GCGAGGACAC CTACGACCTG CCCGACCTCG TGGCCGCGCT CGAAGCGCAC GGGGACATGC
  361 CGCCCAAGAG CTGGACGCGC ACCAAACGCG CCCGCGAGGT CGGAGTGGGC CGCAACTGCA
  421 CCCTGTTCGA CGAGGCCCGC ACCCTCGCCT ACCGCGAGGT GCGCCGACTG CCTGACCGCA
  481 CGCCCGCCTC CTCCGACCTG CTGCGCGAGT ACGTGCGCCG CACCTGCCAC GAAATCAACG
  541 CCTCGTTCCC CGACCCGCTG CCCGTGCGCG AGGTCAACGA CACCGCCAAG AGCATCCACA
  601 AGTGGATCAC CACGCGAAGC CGCATGTGGC GAGACGGTGC CGTCGCCAAC GCCGCCACGT
  661 TCGTCGCCAT CCAATCCGCA CGCGGAAAGA AATCGGGCGA GGCCAGACAA AACGCATTTG
  721 AAGAGAAGTT CGCGCAGTAC GCGCAGGAGG TGCTTGGACA ATGACCATCC AGACAATTCG
  781 CAAGAAGCGC CCATTGCCCG CCAAGGAGTT AGCCGAGGCC TATGGGGGTG TCCGTGCGCA
  841 CGATCAAGTA CTGGAATTCG CAAACGCGCG AGGACTGGAT AGACGAGCAG GCAACGTTGC
  901 GCGAATCCAT CCGCGCCTAC CACGACGATG ACGGCCACTC GTGGAGCCAG ACCGCCGAAC
  961 ACTTCAACAT GACCCAAAGG TGCCGTGCGC CCAACGCGCC CTACCGCGCC CGCAAAGGAA
 1021 ACGCGAGGCC GAGGCAAAAG CCGCAAAGGC CCGGGAGTAG CAGTGACGTG AACGTATAAT
1081 ACGATCAACT TCGAATCGCG GCTGCCGAAA TCGGCCTTGT CGGGAAGGAA T
//
Primer: pTBR101CM_seq7 | Sequence: RDB15974_A8Fbb.seq
>D04829A3-12_A8Fb_pTBR101CM_seq7_G01_07_ABI08.ab1
    1 CAATTGCGCG GATCATCCGC GCCTACCACG ACGATGACGG CCACTCGTGG AGCCAGACCG
   61 CCGAACACTT CAACATGACC CAAGGTGCCG TGCGCCAACG CGCCTACCGC GCCCGCAAGG
  121 AACGCGAGGC CGAGGCAAAA GCCGCAAGGC CGGAGTAGCA AGTGACGTGA ACGTATATAC
  181 GTTCACTCGA TTCGCGGTGC GAATCGGCTT GCCGGGAAGA CCCATGCCCC ATAGTCGGCA
  241 GAGCCTCCAG ACCGCTCACG CGGCATCGTG CCACGACTGT GGCACATCAC GTAACACCCG
  301 CACATCCTCA CGGTGGTGCG CGGGTGTTAC GTGATTCGAA CCATCGGCCT GACAGGTCTC
  361 AGATCGCTCC AGCGAGCTTA CGGCGCATGT ACTCGCTGCG GCTGATGTGG TACCGCCTGG
  421 CCATCGCGGT CAGACCGTCC AGCGTGCTCT TGGGCATGCG GACGGACACG CTGACCATCT
  481 GCTCGTCGGG CTCGGCGAGA TGGAGCCCGT AGTACACGCG CCCCGTCAGG TCGTCCGGGA
  541 CAGTCTCGGA CTCGGCCATC CGCTCATCCT CCAGCACCTG CTCCTCGGTC AGCCCGACCA
  601 GCGCCATCAA CTGCCTGTCG CGTTCGCTGA AATCACTCAT AGCGTCCTCC CATCGCCGCG
  661 CAGACGGTCT ATCTCGCGCC TGAACTTCTT GGTCGGCGGC GTGAACGCGT GGTAGATCAC
  721 CACGAGGTCG CCGACGACCC TGTACAGCAT CTCGACGTTC CTGCCGCGCC CGTCGAGCCC
  781 TATCGCCATC CACGCGCCGG ACTCGCGCTC CGCGTCCACC ATGACGCTGC GGAACGCGGT
  841 CACCACGTCC TTCTCGGACA GTTCTGGGTG ACGCTCCATC ACCCTGTCAA GCACCTGGAT
  901 CCCCGGGTAC CGAGCTCGAA TTCTGAGCAA AAGGCCAGCA AAAGGCCAGG GACCGTAAAA
  961 AGGCCGCGTT GCTGGGCGTT TTTCCATAGC TCCCGCCCCC CTGACGAGCA TCACAAAATC
 1021 GACGCTCAAG GTCAGAGTGC GAAACCCGAC AGGAACTATA AGGATACCAA GGGCGGTTTC
1081 CCCCTTGGAG GCTCCTTCGT TGCGCTCCTC TTGGTCGAAA CCCTGGTCCG CCTA
//
Primer: pTBR101CM_seq3 | Sequence: RDB15974_A8Fbc.seq
>D04829A3-12_A8Fb_pTBR101CM_seq3_F01_06_ABI08.ab1
    1 TGTCCGGGAA GCGCCGCGAA ACGGGTCTGG AGGCTCCGCC GACCATGGGG CATGGGTCTT
   61 CCCGGCAAGC CGATTCGCAC CGCGAATCGA GTGATTGTAG CTACAATCAC GTCGCTTGCT
  121 ACTCCGGCCT TCGGCACTCG GGTTGTTGCT CCTGCGCCGA AGGGCGAGGG AGGCTGCGGG
  181 CTCGGTTCAT GTACATGAAC CGTCTAGCTT GCTTACCTTC GATTTGATGG ACATCTCCAT
  241 GTGGATGTCC ATGGAGATGT CCATGGAGAT GTCCATGGAG ATGTCCATGG AGATGTCCAT
  301 GGAGATGTCC ATGTGGGTGT CCATGTGGGT GTCCATGGAT ATATCCATCT GAGCGCATAG
  361 GAAAACGCAT GAGGGTGGAT GATCGTTCGG GGTGATGCCT TCTTCCACGA ACGGGAAGGG
  421 GCTCTTCCTT TTTCTGGAAT GTCATCTTCC AGCGCCCTTC GCCTTGAGCT CCTTGAGCAG
  481 CATCGTCGCG TTGTCGCGGA TGCTCGCGTC CGTGAGCGGG GAGAGCTCGT CCGCATAGGA
  541 GCGGCAGAGG AGCGCGTACA CCCGTTCCCG CACCCAGGCG CTGCGCGCGA TGCCCAGCTT
  601 GAACGCGGCC CGGCACAGCA TCTCGTATTC GTCGCCGGTG AGCAGGACAT TGAGCTGGCG
  661 TCAAAAGTTG ATTAGTCATG CCGTGTATAT ACGTCCGGGG TGTTTTCGGC CAGATTCCAA
  721 CAGTTTTGGG CCACTCCGGG AAGGAACAGC CGACGCTTCG CTGGGCTGAA CCATCTGACT
  781 TGGTTCGGCG CACGCTTGTG CAATGCACAA ATGTGCAATA GGATGTGAGC TATGAGCAAC
  841 GAATATGTGC AGGGGGACGC TGGAGCTGAC GCGGGCGTTC GACGGCTGGT GGCTTCCCCA
  901 GCGTCCCCTG TGCTGCGACG ATGACTACTC CCAGCTGGTG CGCCGGAGCC GCATCGACGC
  961 GCTCAGATGC AAGCACATCG AGGCGAATCC CGGCAGCGCA GGTGAAACAT GCTCGTTGTT
 1021 GACGTTTGAA TTTCGTCTGA GGACCCGTTT AATGAGCCTG GTTGGGGGGC ATCGGGACAT
1081 GGCCGGCCGA ACTTCAATCG GCGAGAATTC GGCCAACGGA CCACCGTGTC CAACGGC
//
Primer: ColE1ori_F | Sequence: RDB15974_A8Fbd.seq
>D04829A3-12_A8Fb_ColE1ori_F_H01_08_ABI08.ab1
    1 CCCCATGTCT GTCGGATCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
   61 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTCC CTGGCCTTTT
  121 GCTGGCCTTT TGCTCAGAAT TCGAGCTCGG TACCCGGGGA TCCAGGTGCT TGACAGGGTG
  181 ATGGAGCGTC ACCCAGAACT GTCCGAGAAG GACGTGGTGA CCGCGTTCCG CAGCGTCATG
  241 GTGGACGCGG AGCGCGAGTC CGGCGCGTGG ATGGCGATAG GGCTCGACGG GCGCGGCAGG
  301 AACGTCGAGA TGCTGTACAG GGTCGTCGGC GACCTCGTGG TGATCTACCA CGCGTTCACG
  361 CCGCCGACCA AGAAGTTCAG GCGCGAGATA GACCGTCTGC GCGGCGATGG GAGGACGCTA
  421 TGAGTGATTT CAGCGAACGC GACAGGCAGT TGATGGCGCT GGTCGGGCTG ACCGAGGAGC
  481 AGGTGCTGGA GGATGAGCGG ATGGCCGAGT CCGAGACTGT CCCGGACGAC CTGACGGGGC
  541 GCGTGTACTA CGGGCTCCAT CTCGCCGAGC CCGACGAGCA GATGGTCAGC GTGTCCGTCC
  601 GCATGCCCAA GAGCACGCTG GACGGTCTGA CCGCGATGGC CAGGCGGTAC CACATCAGCC
  661 GCAGCGAGTA CATGCGCCGT AAGCTCGCTG GAGCGATCTG AGACCTGTCA GGCCGATGGT
  721 TCGAATCACG TAACACCCGC GCACCACCGT GAGGATGTGC GGGTGTTACG TGATGTGCCA
  781 CAGTCGTGGC ACGATGCCGC GTGAGCGGTC TGGAGGGCTC TGCCGACTAT GGGGCATGGG
  841 TCTTCCCGGC AAGCCGATTC GCACCGCGGA ATCGAGTGGA ACGTATATAA CGTTCACGTC
  901 ACTTGCTACT TCCGGGCCTT TGCGGGCTTT TGCCTCGGCC CTCGCGTTTC CTTTGCGGGC
  961 GCCGGTAGGG CGCGTTTGGC GCACGGCAAC CTTTGGGTTC AATGTTTGAA AGTGGTTCGG
 1021 CCGGTTCTTG GTTCCACGAG TGGGCCCGGT CAATCGTCGT TGGTAAGCGC GGATGGGAAT
 1081 TTCGCGCAAC GGTTGGCTTG CTCGTCTATC AGGTCCTCGC CGCGATTTGC GCGAAAATCC
1141 GGATTATGCT ATGGGATGTC CGTGT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061.
reference Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016).

2019.11.02

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