Resource data sheet
Prev.

pBS423 delta repA

Bifidobacterium longum–E. coli shuttle vector.

Catalog number RDB15973
Resource name pBS423 delta repA
Clone info. Bifidobacterium longum–E. coli shuttle vector. Conditional replication (not to replicate) for the first crossover event in B. longum 105-A. Please note that pBS423 delta repA is hard to be replicated in B. longum 105-A strain. Please refer Genbank accession AB687999 for information of the original pBS423.
The depositor uses spectinomycin at a concentration of 75 microgram/mL for selection of transformants in agar media. B. longum 105-A (JCM 31944) is available from JCM as a host strain.
Vector backbone pBS423 delta repA (Plasmid)
Size of vector backbone 4.4 kb
Selectable markers SpR
Depositor Fukiya, Satoru | Kano, Yasunobu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
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RDB15973 pBS423 delta repA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Bacteria host strains (en) (English text)
Featured content Bacteria host strains (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15973_A8Fbp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBS423_seq3 | Sequence: RDB15973_A8Fba.seq
>D04829A2-14_A8Fb_pBS423_seq3_A01_01_ABI08.ab1
    1 TGGCCGGGGC AGGGGACATG CTCGTTGTTA ACGACACCGC CAAGAGCATC CACAAGTGGA
   61 TCACCACGCG AAGCCGCATG TGGCGAGACG GTGCCGTCGC CAACGCCGCC ACGTTCGTCG
  121 CCATCCAATC CGCACGCGGA AAGAAATCGG GCGAGGCCAG ACAAAACGCA TTTGAAGAGA
  181 AGTTCGCGCA GTACGCGCAG GAGGTGCTTG GACAATGACC ATCCAGACAA TTCGCAAGAA
  241 GCGCCCATTG CCCGCCAAGG AGTTAGCCGA GGCCTATGGG GTGTCCGTGC GCACGATCAA
  301 GTACTGGAAT TCGCAAACGC GCGAGGACTG GATAGACGAG CAGGCAACGT TGCGCGAATC
  361 CATCCGCGCC TACCACGACG ATGACGGCCA CTCGTGGAGC CAGACCGCCG AACACTTCAA
  421 CATGACCCAA GGTGCCGTGC GCCAACGCGC CTACCGCGCC CGCAAGGAAC GCGAGGCCGA
  481 GGCAAAAGCC GCAAGGCCGG AGTAGCAAGT GACGTGAACG TATATACGTT CACTCGATTC
  541 GCGGTGCGAA TCGGCTTGCC GGGAAGACCC ATGCCCCATA GTCGGCAGAG CCTCCAGACC
  601 GCTCACGCGG CATCGTGCCA CGACTGTGGC ACATCACGTA ACACCCGCAC ATCCTCACGG
  661 TGGTGCGCGG GTGTTACGTG ATTCGAACCA TCGGCCTGAC AGGTCTCAGA TCGCTCCAGC
  721 GAGCTTACGG CGCATGTACT CGCTGCGGCT GATGTGGTAC CGCCTGGCCA TCGCGGTCAG
  781 ACCGTCCAGC GTGCTCTTGG GCATGCGGAC GGACACGCTG ACCATCTGCT CGTCGGGCTC
  841 GGCGAGATGG AGCCCGTAGT ACACGCGCCC CGTCAGGTCG TCCGGGACAG TCTCGGACTC
  901 GGCCATCCGC TCATCCTCCA GCACCTGCTC CTCGGTCAGC CCGACCAGCG CCATCAACTG
  961 CCTGTCGCGT TCGCTGAAAA TCACTCATAG CGTCCTCCCA TCGCCGCGCA GACGGGTCTA
 1021 TCTCGCGCCC TGAACTTCTT GGTCGGCGGG CGTGAAACGC GTGGGTAGAT CCACCCACGA
1081 AGTTCGACCG AACGACCCCT GGTACCAGGC CAATTCCTCG ACGGATTGCC TGATG
//
Primer: pTBR101CM_seq7 | Sequence: RDB15973_A8Fbb.seq
>D04829A2-14_A8Fb_pTBR101CM_seq7_C01_03_ABI08.ab1
    1 CAAAGGCGGG CGGAATCATC CGCGCCTACC ACGACGATGA CGGCCACTCG TGGAGCCAGA
   61 CCGCCGAACA CTTCAACATG ACCCAAGGTG CCGTGCGCCA ACGCGCCTAC CGCGCCCGCA
  121 AGGAACGCGA GGCCGAGGCA AAAGCCGCAA GGCCGGAGTA GCAAGTGACG TGAACGTATA
  181 TACGTTCACT CGATTCGCGG TGCGAATCGG CTTGCCGGGA AGACCCATGC CCCATAGTCG
  241 GCAGAGCCTC CAGACCGCTC ACGCGGCATC GTGCCACGAC TGTGGCACAT CACGTAACAC
  301 CCGCACATCC TCACGGTGGT GCGCGGGTGT TACGTGATTC GAACCATCGG CCTGACAGGT
  361 CTCAGATCGC TCCAGCGAGC TTACGGCGCA TGTACTCGCT GCGGCTGATG TGGTACCGCC
  421 TGGCCATCGC GGTCAGACCG TCCAGCGTGC TCTTGGGCAT GCGGACGGAC ACGCTGACCA
  481 TCTGCTCGTC GGGCTCGGCG AGATGGAGCC CGTAGTACAC GCGCCCCGTC AGGTCGTCCG
  541 GGACAGTCTC GGACTCGGCC ATCCGCTCAT CCTCCAGCAC CTGCTCCTCG GTCAGCCCGA
  601 CCAGCGCCAT CAACTGCCTG TCGCGTTCGC TGAAATCACT CATAGCGTCC TCCCATCGCC
  661 GCGCAGACGG TCTATCTCGC GCCTGAACTT CTTGGTCGGC GGCGTGAACG CGTGGTAGAT
  721 CACCACGAGG TCGCCGACGA CCCTGTACAG CATCTCGACG TTCCTGCCGC GCCCGTCGAG
  781 CCCTATCGCC ATCCACGCGC CGGACTCGCG CTCCGCGTCC ACCATGACGC TGCGGAACGC
  841 GGTCACCACG TCCTTCTCGG ACAGTTCTGG GGTGACGCTC CATCACCCTG TCAAGCACCT
  901 GGATCCCCGG GTACCGAGCT CGAATTCTGA GCAAAAGGCC AGCAAAAGGC CAGGGACCGT
  961 AAAAAAGGCC GCGTTGCTGG CGTTTTTTCC ATAGGCTCCG CCCCCCCTGA CGAGCATCAC
 1021 AAAATCCGAC GGCTCAAGTC AGAGGTGGCG AAACCCCGGA ACAGAACTAT AAGATACCAG
1081 GCGTTCCCCC TGAGCTCCCT CGATGCGCCT CCTTCCATGT TTTTCCCGAC
//
Primer: pTBR101CM_seq3 | Sequence: RDB15973_A8Fbc.seq
>D04829A2-14_A8Fb_pTBR101CM_seq3_B01_02_ABI08.ab1
    1 TGTCGGTTAA GCGCGCGAGC GGTCTGGAGG CTCCGCCGAC CATGGGGCAT GGGTCTTCCC
   61 GGCAAGCCGA TTCGCACCGC GAATCGAGTG ATTGTAGCTA CAATCACGTC GCTTGCTACT
  121 CCGGCCTTCG GCACTCGGGT TGTTGCTCCT GCGCCGAAGG GCGAGGGAGG CTGCGGGCTC
  181 GGTTCATGTA CATGAACCGT CTAGCTTGCT TACCTTCGAT TTGATGGACA TCTCCATGTG
  241 GATGTCCATG GAGATGTCCA TGGAGATGTC CATGGAGATG TCCATGGAGA TGTCCATGGA
  301 GATGTCCATG TGGGTGTCCA TGTGGGTGTC CATGGATATA TCCATCTGAG CGCATAGGAA
  361 AACGCATGAG GGTGGATGAT CGTTCGGGGT GATGCCTTCT TCCACGAACG GGAAGGGGCT
  421 CTTCCTTTTT CTGGAATGTC ATCTTCCAGC GCCCTTCGCC TTGAGCTCCT TGAGCAGCAT
  481 CGTCGCGTTG TCGCGGATGC TCGCGTCCGT GAGCGGGGAG AGCTCGTCCG CATAGGAGCG
  541 GCAGAGGAGC GCGTACACCC GTTCCCGCAC CCAGGCGCTG CGCGCGATGC CCAGCTTGAA
  601 CGCGGCCCGG CACAGCATCT CGTATTCGTC GCCGGTGAGC AGGACATTGA GCTGGCGTCA
  661 AAAGTTGATT AGTCATGCCG TGTATATACG TCCGGGGTGT TTTCGGCCAG ATTCCAACAG
  721 TTTTGGGCCA CTCCGGGAAG GAACAGCCGA CGCTTCGCTG GGCTGAACCA TCTGACTTGG
  781 TTCGGCGCAC GCTTGTGCAA TGCACAAATG TGCAATAGGA TGTGAGCTAT GAGCAACGAA
  841 TATGTGCAGG GGACGCTGGA GCTGACGCGG GCGTTCGACG GCTGGTGGCT TCCCCAGCGT
  901 CCCCTGTGCT GCGACGATGA CTACTCCCAG CTGGTGCGCC GGAGCCGCAT CGACGCGCTC
  961 AGATGCAAGC ACATCGAGGC GAAATCCGGG CAGCGCAGGT GAACATGCTC GTTTGTTAAC
 1021 GACACCCGCC CAGAGCATCC ACAAGTGGAT CACCAACGCG GAGCCGCATG TGGCGAGACG
1081 GTGGCGTCGC TACGACGGCA CGATCGATCG GCCATTCCCA ATTCG
//
Primer: ColE1ori_F | Sequence: RDB15973_A8Fbd.seq
>D04829A2-14_A8Fb_ColE1ori_F_D01_04_ABI08.ab1
    1 GGTATGATCT GTCGACGCCA CCTCTGACTT GAGCGTCGGA TTTTTGTGAT GCTCGTCAGG
   61 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTCCC TGGCCTTTTG
  121 CTGGCCTTTT GCTCAGAATT CGAGCTCGGT ACCCGGGGAT CCAGGTGCTT GACAGGGTGA
  181 TGGAGCGTCA CCCAGAACTG TCCGAGAAGG ACGTGGTGAC CGCGTTCCGC AGCGTCATGG
  241 TGGACGCGGA GCGCGAGTCC GGCGCGTGGA TGGCGATAGG GCTCGACGGG CGCGGCAGGA
  301 ACGTCGAGAT GCTGTACAGG GTCGTCGGCG ACCTCGTGGT GATCTACCAC GCGTTCACGC
  361 CGCCGACCAA GAAGTTCAGG CGCGAGATAG ACCGTCTGCG CGGCGATGGG AGGACGCTAT
  421 GAGTGATTTC AGCGAACGCG ACAGGCAGTT GATGGCGCTG GTCGGGCTGA CCGAGGAGCA
  481 GGTGCTGGAG GATGAGCGGA TGGCCGAGTC CGAGACTGTC CCGGACGACC TGACGGGGCG
  541 CGTGTACTAC GGGCTCCATC TCGCCGAGCC CGACGAGCAG ATGGTCAGCG TGTCCGTCCG
  601 CATGCCCAAG AGCACGCTGG ACGGTCTGAC CGCGATGGCC AGGCGGTACC ACATCAGCCG
  661 CAGCGAGTAC ATGCGCCGTA AGCTCGCTGG AGCGATCTGA GACCTGTCAG GCCGATGGTT
  721 CGAATCACGT AACACCCGCG CACCACCGTG AGGGATGTGC GGGTGTTTAC GTGGATGTGC
  781 CCACAGTCGT GGCACGATGG CCGCGGTGAG CGGTCTGGAA GGCTCTGCCC GACTATGGGG
  841 GCATGGGTCT TTCCCGGGCA AGCCCGAATT CGCACCGGCG AATTCGGAGT GAACGTAATA
  901 TACGTTGCAC GTCACTTTGC CTACTCCGGG CCCTTGCGGC TTTTTGCGCT CGGGCCTCGC
961 GTTTCCCTTG CGGGCGCCGG TAGGGGG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061.
reference O'Connell Motherway, M., Carbohydrate Syntrophy enhances the establishment of Bifidobacterium breve UCC2003 in the neonatal gut. Sci. Rep. 8 (1): 10627 (2018). PMID 30006512.
reference Nishiyama, K., Bifidobacterium bifidum Extracellular Sialidase Enhances Adhesion to the Mucosal Surface and Supports Carbohydrate Assimilation. MBio 8 (5). pii: e00928-17 (2017). PMID 28974612.
reference Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016).

2019.11.02

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