RDB15973 - pBS423 delta repA

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pBS423 delta repA

Bifidobacterium longum–E. coli shuttle vector.

Catalog number	RDB15973
Resource name	pBS423 delta repA
Clone info.	Bifidobacterium longum–E. coli shuttle vector. Conditional replication (not to replicate) for the first crossover event in B. longum 105-A. Please note that pBS423 delta repA is hard to be replicated in B. longum 105-A strain. Please refer Genbank accession AB687999 for information of the original pBS423.
Comment	The depositor uses spectinomycin at a concentration of 75 microgram/mL for selection of transformants in agar media. B. longum 105-A (JCM 31944) is available from JCM as a host strain.
Vector backbone	pBS423 delta repA (Plasmid)
Size of vector backbone	4.4 kb
Selectable markers	SpR
Depositor\|Developer	Fukiya, Satoru \| Kano, Yasunobu \|

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External Database

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.

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提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは，非営利の学術研究に限って提供する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15973	pBS423 delta repA	DNA solution

Please review the QC test results indicated by check

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References and tips

Electronic file

Original reference

original	Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061.

Further references such as user reports and related articles (go to bottom)

Featured content	Bacteria host strains (en) (English text)
Featured content	Bacteria host strains (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15973_A8Fbp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBS423_seq3 Sequence file: RDB15973_A8Fba.seq
>D04829A2-14_A8Fb_pBS423_seq3_A01_01_ABI08.ab1 1 TGGCCGGGGC AGGGGACATG CTCGTTGTTA ACGACACCGC CAAGAGCATC CACAAGTGGA 61 TCACCACGCG AAGCCGCATG TGGCGAGACG GTGCCGTCGC CAACGCCGCC ACGTTCGTCG 121 CCATCCAATC CGCACGCGGA AAGAAATCGG GCGAGGCCAG ACAAAACGCA TTTGAAGAGA 181 AGTTCGCGCA GTACGCGCAG GAGGTGCTTG GACAATGACC ATCCAGACAA TTCGCAAGAA 241 GCGCCCATTG CCCGCCAAGG AGTTAGCCGA GGCCTATGGG GTGTCCGTGC GCACGATCAA 301 GTACTGGAAT TCGCAAACGC GCGAGGACTG GATAGACGAG CAGGCAACGT TGCGCGAATC 361 CATCCGCGCC TACCACGACG ATGACGGCCA CTCGTGGAGC CAGACCGCCG AACACTTCAA 421 CATGACCCAA GGTGCCGTGC GCCAACGCGC CTACCGCGCC CGCAAGGAAC GCGAGGCCGA 481 GGCAAAAGCC GCAAGGCCGG AGTAGCAAGT GACGTGAACG TATATACGTT CACTCGATTC 541 GCGGTGCGAA TCGGCTTGCC GGGAAGACCC ATGCCCCATA GTCGGCAGAG CCTCCAGACC 601 GCTCACGCGG CATCGTGCCA CGACTGTGGC ACATCACGTA ACACCCGCAC ATCCTCACGG 661 TGGTGCGCGG GTGTTACGTG ATTCGAACCA TCGGCCTGAC AGGTCTCAGA TCGCTCCAGC 721 GAGCTTACGG CGCATGTACT CGCTGCGGCT GATGTGGTAC CGCCTGGCCA TCGCGGTCAG 781 ACCGTCCAGC GTGCTCTTGG GCATGCGGAC GGACACGCTG ACCATCTGCT CGTCGGGCTC 841 GGCGAGATGG AGCCCGTAGT ACACGCGCCC CGTCAGGTCG TCCGGGACAG TCTCGGACTC 901 GGCCATCCGC TCATCCTCCA GCACCTGCTC CTCGGTCAGC CCGACCAGCG CCATCAACTG 961 CCTGTCGCGT TCGCTGAAAA TCACTCATAG CGTCCTCCCA TCGCCGCGCA GACGGGTCTA 1021 TCTCGCGCCC TGAACTTCTT GGTCGGCGGG CGTGAAACGC GTGGGTAGAT CCACCCACGA 1081 AGTTCGACCG AACGACCCCT GGTACCAGGC CAATTCCTCG ACGGATTGCC TGATG //
Primer: pTBR101CM_seq7 Sequence file: RDB15973_A8Fbb.seq
>D04829A2-14_A8Fb_pTBR101CM_seq7_B01_02_ABI08.ab1 1 TGTCGGTTAA GCGCGCGAGC GGTCTGGAGG CTCCGCCGAC CATGGGGCAT GGGTCTTCCC 61 GGCAAGCCGA TTCGCACCGC GAATCGAGTG ATTGTAGCTA CAATCACGTC GCTTGCTACT 121 CCGGCCTTCG GCACTCGGGT TGTTGCTCCT GCGCCGAAGG GCGAGGGAGG CTGCGGGCTC 181 GGTTCATGTA CATGAACCGT CTAGCTTGCT TACCTTCGAT TTGATGGACA TCTCCATGTG 241 GATGTCCATG GAGATGTCCA TGGAGATGTC CATGGAGATG TCCATGGAGA TGTCCATGGA 301 GATGTCCATG TGGGTGTCCA TGTGGGTGTC CATGGATATA TCCATCTGAG CGCATAGGAA 361 AACGCATGAG GGTGGATGAT CGTTCGGGGT GATGCCTTCT TCCACGAACG GGAAGGGGCT 421 CTTCCTTTTT CTGGAATGTC ATCTTCCAGC GCCCTTCGCC TTGAGCTCCT TGAGCAGCAT 481 CGTCGCGTTG TCGCGGATGC TCGCGTCCGT GAGCGGGGAG AGCTCGTCCG CATAGGAGCG 541 GCAGAGGAGC GCGTACACCC GTTCCCGCAC CCAGGCGCTG CGCGCGATGC CCAGCTTGAA 601 CGCGGCCCGG CACAGCATCT CGTATTCGTC GCCGGTGAGC AGGACATTGA GCTGGCGTCA 661 AAAGTTGATT AGTCATGCCG TGTATATACG TCCGGGGTGT TTTCGGCCAG ATTCCAACAG 721 TTTTGGGCCA CTCCGGGAAG GAACAGCCGA CGCTTCGCTG GGCTGAACCA TCTGACTTGG 781 TTCGGCGCAC GCTTGTGCAA TGCACAAATG TGCAATAGGA TGTGAGCTAT GAGCAACGAA 841 TATGTGCAGG GGACGCTGGA GCTGACGCGG GCGTTCGACG GCTGGTGGCT TCCCCAGCGT 901 CCCCTGTGCT GCGACGATGA CTACTCCCAG CTGGTGCGCC GGAGCCGCAT CGACGCGCTC 961 AGATGCAAGC ACATCGAGGC GAAATCCGGG CAGCGCAGGT GAACATGCTC GTTTGTTAAC 1021 GACACCCGCC CAGAGCATCC ACAAGTGGAT CACCAACGCG GAGCCGCATG TGGCGAGACG 1081 GTGGCGTCGC TACGACGGCA CGATCGATCG GCCATTCCCA ATTCG //
Primer: pTBR101CM_seq3 Sequence file: RDB15973_A8Fbc.seq
>D04829A2-14_A8Fb_pTBR101CM_seq3_C01_03_ABI08.ab1 1 CAAAGGCGGG CGGAATCATC CGCGCCTACC ACGACGATGA CGGCCACTCG TGGAGCCAGA 61 CCGCCGAACA CTTCAACATG ACCCAAGGTG CCGTGCGCCA ACGCGCCTAC CGCGCCCGCA 121 AGGAACGCGA GGCCGAGGCA AAAGCCGCAA GGCCGGAGTA GCAAGTGACG TGAACGTATA 181 TACGTTCACT CGATTCGCGG TGCGAATCGG CTTGCCGGGA AGACCCATGC CCCATAGTCG 241 GCAGAGCCTC CAGACCGCTC ACGCGGCATC GTGCCACGAC TGTGGCACAT CACGTAACAC 301 CCGCACATCC TCACGGTGGT GCGCGGGTGT TACGTGATTC GAACCATCGG CCTGACAGGT 361 CTCAGATCGC TCCAGCGAGC TTACGGCGCA TGTACTCGCT GCGGCTGATG TGGTACCGCC 421 TGGCCATCGC GGTCAGACCG TCCAGCGTGC TCTTGGGCAT GCGGACGGAC ACGCTGACCA 481 TCTGCTCGTC GGGCTCGGCG AGATGGAGCC CGTAGTACAC GCGCCCCGTC AGGTCGTCCG 541 GGACAGTCTC GGACTCGGCC ATCCGCTCAT CCTCCAGCAC CTGCTCCTCG GTCAGCCCGA 601 CCAGCGCCAT CAACTGCCTG TCGCGTTCGC TGAAATCACT CATAGCGTCC TCCCATCGCC 661 GCGCAGACGG TCTATCTCGC GCCTGAACTT CTTGGTCGGC GGCGTGAACG CGTGGTAGAT 721 CACCACGAGG TCGCCGACGA CCCTGTACAG CATCTCGACG TTCCTGCCGC GCCCGTCGAG 781 CCCTATCGCC ATCCACGCGC CGGACTCGCG CTCCGCGTCC ACCATGACGC TGCGGAACGC 841 GGTCACCACG TCCTTCTCGG ACAGTTCTGG GGTGACGCTC CATCACCCTG TCAAGCACCT 901 GGATCCCCGG GTACCGAGCT CGAATTCTGA GCAAAAGGCC AGCAAAAGGC CAGGGACCGT 961 AAAAAAGGCC GCGTTGCTGG CGTTTTTTCC ATAGGCTCCG CCCCCCCTGA CGAGCATCAC 1021 AAAATCCGAC GGCTCAAGTC AGAGGTGGCG AAACCCCGGA ACAGAACTAT AAGATACCAG 1081 GCGTTCCCCC TGAGCTCCCT CGATGCGCCT CCTTCCATGT TTTTCCCGAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061.
reference	O'Connell Motherway, M., Carbohydrate Syntrophy enhances the establishment of Bifidobacterium breve UCC2003 in the neonatal gut. Sci. Rep. 8 (1): 10627 (2018). PMID 30006512.
reference	Nishiyama, K., Bifidobacterium bifidum Extracellular Sialidase Enhances Adhesion to the Mucosal Surface and Supports Carbohydrate Assimilation. MBio 8 (5). pii: e00928-17 (2017). PMID 28974612.
reference	Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016).

2022.05.17

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