Sequencing and PCR primers for primer information.
Bifidobacterium longum–E. coli shuttle vector.
| Catalog number | RDB15973 |
|---|---|
| Resource name | pBS423 delta repA |
| Clone info. | Bifidobacterium longum–E. coli shuttle vector. Conditional replication (not to replicate) for the first crossover event in B. longum 105-A. Please note that pBS423 delta repA is hard to be replicated in B. longum 105-A strain. Please refer Genbank accession AB687999 for information of the original pBS423. The depositor uses spectinomycin at a concentration of 75 microgram/mL for selection of transformants in agar media. B. longum 105-A (JCM 31944) is available from JCM as a host strain. |
| Vector backbone | pBS423 delta repA (Plasmid) |
| Size of vector backbone | 4.4 kb |
| Selectable markers | SpR |
| Depositor | Fukiya, Satoru | Kano, Yasunobu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15973 | pBS423 delta repA | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Bacteria host strains (en) (English text) |
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| Featured content | Bacteria host strains (ja) (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15973_A8Fbp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pBS423_seq3 | Sequence: RDB15973_A8Fba.seq |
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>D04829A2-14_A8Fb_pBS423_seq3_A01_01_ABI08.ab1 1 TGGCCGGGGC AGGGGACATG CTCGTTGTTA ACGACACCGC CAAGAGCATC CACAAGTGGA 61 TCACCACGCG AAGCCGCATG TGGCGAGACG GTGCCGTCGC CAACGCCGCC ACGTTCGTCG 121 CCATCCAATC CGCACGCGGA AAGAAATCGG GCGAGGCCAG ACAAAACGCA TTTGAAGAGA 181 AGTTCGCGCA GTACGCGCAG GAGGTGCTTG GACAATGACC ATCCAGACAA TTCGCAAGAA 241 GCGCCCATTG CCCGCCAAGG AGTTAGCCGA GGCCTATGGG GTGTCCGTGC GCACGATCAA 301 GTACTGGAAT TCGCAAACGC GCGAGGACTG GATAGACGAG CAGGCAACGT TGCGCGAATC 361 CATCCGCGCC TACCACGACG ATGACGGCCA CTCGTGGAGC CAGACCGCCG AACACTTCAA 421 CATGACCCAA GGTGCCGTGC GCCAACGCGC CTACCGCGCC CGCAAGGAAC GCGAGGCCGA 481 GGCAAAAGCC GCAAGGCCGG AGTAGCAAGT GACGTGAACG TATATACGTT CACTCGATTC 541 GCGGTGCGAA TCGGCTTGCC GGGAAGACCC ATGCCCCATA GTCGGCAGAG CCTCCAGACC 601 GCTCACGCGG CATCGTGCCA CGACTGTGGC ACATCACGTA ACACCCGCAC ATCCTCACGG 661 TGGTGCGCGG GTGTTACGTG ATTCGAACCA TCGGCCTGAC AGGTCTCAGA TCGCTCCAGC 721 GAGCTTACGG CGCATGTACT CGCTGCGGCT GATGTGGTAC CGCCTGGCCA TCGCGGTCAG 781 ACCGTCCAGC GTGCTCTTGG GCATGCGGAC GGACACGCTG ACCATCTGCT CGTCGGGCTC 841 GGCGAGATGG AGCCCGTAGT ACACGCGCCC CGTCAGGTCG TCCGGGACAG TCTCGGACTC 901 GGCCATCCGC TCATCCTCCA GCACCTGCTC CTCGGTCAGC CCGACCAGCG CCATCAACTG 961 CCTGTCGCGT TCGCTGAAAA TCACTCATAG CGTCCTCCCA TCGCCGCGCA GACGGGTCTA 1021 TCTCGCGCCC TGAACTTCTT GGTCGGCGGG CGTGAAACGC GTGGGTAGAT CCACCCACGA 1081 AGTTCGACCG AACGACCCCT GGTACCAGGC CAATTCCTCG ACGGATTGCC TGATG // |
| Primer: pTBR101CM_seq7 | Sequence: RDB15973_A8Fbb.seq |
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>D04829A2-14_A8Fb_pTBR101CM_seq7_C01_03_ABI08.ab1 1 CAAAGGCGGG CGGAATCATC CGCGCCTACC ACGACGATGA CGGCCACTCG TGGAGCCAGA 61 CCGCCGAACA CTTCAACATG ACCCAAGGTG CCGTGCGCCA ACGCGCCTAC CGCGCCCGCA 121 AGGAACGCGA GGCCGAGGCA AAAGCCGCAA GGCCGGAGTA GCAAGTGACG TGAACGTATA 181 TACGTTCACT CGATTCGCGG TGCGAATCGG CTTGCCGGGA AGACCCATGC CCCATAGTCG 241 GCAGAGCCTC CAGACCGCTC ACGCGGCATC GTGCCACGAC TGTGGCACAT CACGTAACAC 301 CCGCACATCC TCACGGTGGT GCGCGGGTGT TACGTGATTC GAACCATCGG CCTGACAGGT 361 CTCAGATCGC TCCAGCGAGC TTACGGCGCA TGTACTCGCT GCGGCTGATG TGGTACCGCC 421 TGGCCATCGC GGTCAGACCG TCCAGCGTGC TCTTGGGCAT GCGGACGGAC ACGCTGACCA 481 TCTGCTCGTC GGGCTCGGCG AGATGGAGCC CGTAGTACAC GCGCCCCGTC AGGTCGTCCG 541 GGACAGTCTC GGACTCGGCC ATCCGCTCAT CCTCCAGCAC CTGCTCCTCG GTCAGCCCGA 601 CCAGCGCCAT CAACTGCCTG TCGCGTTCGC TGAAATCACT CATAGCGTCC TCCCATCGCC 661 GCGCAGACGG TCTATCTCGC GCCTGAACTT CTTGGTCGGC GGCGTGAACG CGTGGTAGAT 721 CACCACGAGG TCGCCGACGA CCCTGTACAG CATCTCGACG TTCCTGCCGC GCCCGTCGAG 781 CCCTATCGCC ATCCACGCGC CGGACTCGCG CTCCGCGTCC ACCATGACGC TGCGGAACGC 841 GGTCACCACG TCCTTCTCGG ACAGTTCTGG GGTGACGCTC CATCACCCTG TCAAGCACCT 901 GGATCCCCGG GTACCGAGCT CGAATTCTGA GCAAAAGGCC AGCAAAAGGC CAGGGACCGT 961 AAAAAAGGCC GCGTTGCTGG CGTTTTTTCC ATAGGCTCCG CCCCCCCTGA CGAGCATCAC 1021 AAAATCCGAC GGCTCAAGTC AGAGGTGGCG AAACCCCGGA ACAGAACTAT AAGATACCAG 1081 GCGTTCCCCC TGAGCTCCCT CGATGCGCCT CCTTCCATGT TTTTCCCGAC // |
| Primer: pTBR101CM_seq3 | Sequence: RDB15973_A8Fbc.seq |
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>D04829A2-14_A8Fb_pTBR101CM_seq3_B01_02_ABI08.ab1 1 TGTCGGTTAA GCGCGCGAGC GGTCTGGAGG CTCCGCCGAC CATGGGGCAT GGGTCTTCCC 61 GGCAAGCCGA TTCGCACCGC GAATCGAGTG ATTGTAGCTA CAATCACGTC GCTTGCTACT 121 CCGGCCTTCG GCACTCGGGT TGTTGCTCCT GCGCCGAAGG GCGAGGGAGG CTGCGGGCTC 181 GGTTCATGTA CATGAACCGT CTAGCTTGCT TACCTTCGAT TTGATGGACA TCTCCATGTG 241 GATGTCCATG GAGATGTCCA TGGAGATGTC CATGGAGATG TCCATGGAGA TGTCCATGGA 301 GATGTCCATG TGGGTGTCCA TGTGGGTGTC CATGGATATA TCCATCTGAG CGCATAGGAA 361 AACGCATGAG GGTGGATGAT CGTTCGGGGT GATGCCTTCT TCCACGAACG GGAAGGGGCT 421 CTTCCTTTTT CTGGAATGTC ATCTTCCAGC GCCCTTCGCC TTGAGCTCCT TGAGCAGCAT 481 CGTCGCGTTG TCGCGGATGC TCGCGTCCGT GAGCGGGGAG AGCTCGTCCG CATAGGAGCG 541 GCAGAGGAGC GCGTACACCC GTTCCCGCAC CCAGGCGCTG CGCGCGATGC CCAGCTTGAA 601 CGCGGCCCGG CACAGCATCT CGTATTCGTC GCCGGTGAGC AGGACATTGA GCTGGCGTCA 661 AAAGTTGATT AGTCATGCCG TGTATATACG TCCGGGGTGT TTTCGGCCAG ATTCCAACAG 721 TTTTGGGCCA CTCCGGGAAG GAACAGCCGA CGCTTCGCTG GGCTGAACCA TCTGACTTGG 781 TTCGGCGCAC GCTTGTGCAA TGCACAAATG TGCAATAGGA TGTGAGCTAT GAGCAACGAA 841 TATGTGCAGG GGACGCTGGA GCTGACGCGG GCGTTCGACG GCTGGTGGCT TCCCCAGCGT 901 CCCCTGTGCT GCGACGATGA CTACTCCCAG CTGGTGCGCC GGAGCCGCAT CGACGCGCTC 961 AGATGCAAGC ACATCGAGGC GAAATCCGGG CAGCGCAGGT GAACATGCTC GTTTGTTAAC 1021 GACACCCGCC CAGAGCATCC ACAAGTGGAT CACCAACGCG GAGCCGCATG TGGCGAGACG 1081 GTGGCGTCGC TACGACGGCA CGATCGATCG GCCATTCCCA ATTCG // |
| Primer: ColE1ori_F | Sequence: RDB15973_A8Fbd.seq |
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>D04829A2-14_A8Fb_ColE1ori_F_D01_04_ABI08.ab1 1 GGTATGATCT GTCGACGCCA CCTCTGACTT GAGCGTCGGA TTTTTGTGAT GCTCGTCAGG 61 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTCCC TGGCCTTTTG 121 CTGGCCTTTT GCTCAGAATT CGAGCTCGGT ACCCGGGGAT CCAGGTGCTT GACAGGGTGA 181 TGGAGCGTCA CCCAGAACTG TCCGAGAAGG ACGTGGTGAC CGCGTTCCGC AGCGTCATGG 241 TGGACGCGGA GCGCGAGTCC GGCGCGTGGA TGGCGATAGG GCTCGACGGG CGCGGCAGGA 301 ACGTCGAGAT GCTGTACAGG GTCGTCGGCG ACCTCGTGGT GATCTACCAC GCGTTCACGC 361 CGCCGACCAA GAAGTTCAGG CGCGAGATAG ACCGTCTGCG CGGCGATGGG AGGACGCTAT 421 GAGTGATTTC AGCGAACGCG ACAGGCAGTT GATGGCGCTG GTCGGGCTGA CCGAGGAGCA 481 GGTGCTGGAG GATGAGCGGA TGGCCGAGTC CGAGACTGTC CCGGACGACC TGACGGGGCG 541 CGTGTACTAC GGGCTCCATC TCGCCGAGCC CGACGAGCAG ATGGTCAGCG TGTCCGTCCG 601 CATGCCCAAG AGCACGCTGG ACGGTCTGAC CGCGATGGCC AGGCGGTACC ACATCAGCCG 661 CAGCGAGTAC ATGCGCCGTA AGCTCGCTGG AGCGATCTGA GACCTGTCAG GCCGATGGTT 721 CGAATCACGT AACACCCGCG CACCACCGTG AGGGATGTGC GGGTGTTTAC GTGGATGTGC 781 CCACAGTCGT GGCACGATGG CCGCGGTGAG CGGTCTGGAA GGCTCTGCCC GACTATGGGG 841 GCATGGGTCT TTCCCGGGCA AGCCCGAATT CGCACCGGCG AATTCGGAGT GAACGTAATA 901 TACGTTGCAC GTCACTTTGC CTACTCCGGG CCCTTGCGGC TTTTTGCGCT CGGGCCTCGC 961 GTTTCCCTTG CGGGCGCCGG TAGGGGG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. |
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| reference | O'Connell Motherway, M., Carbohydrate Syntrophy enhances the establishment of Bifidobacterium breve UCC2003 in the neonatal gut. Sci. Rep. 8 (1): 10627 (2018). PMID 30006512. |
| reference | Nishiyama, K., Bifidobacterium bifidum Extracellular Sialidase Enhances Adhesion to the Mucosal Surface and Supports Carbohydrate Assimilation. MBio 8 (5). pii: e00928-17 (2017). PMID 28974612. |
| reference | Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016). |
2019.11.02
GNP_filter3_RDBDEP_html_190927.pl