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pBS423 delta repA (#RDB15973)

Bifidobacterium longum–E. coli shuttle vector.

Clone info. Bifidobacterium longum–E. coli shuttle vector. Conditional replication (not to replicate) for the first crossover event in B. longum 105-A. Please note that pBS423 delta repA is hard to be replicated in B. longum 105-A strain. Please refer Genbank accession AB687999 for information of the original pBS423.
Comment The depositor uses spectinomycin at a concentration of 75 microgram/mL for selection of transformants in agar media. B. longum 105-A (JCM 31944) is available from JCM as a host strain.
Vector backbone pBS423 delta repA (Plasmid)
Size of vector backbone 4.4 kb
Selectable markers SpR
Depositor|Developer Fukiya, Satoru | Kano, Yasunobu |
 
Sequence (full) RDB15973hts01.seq checkAssembled from experimentally sequenced data.
Publication Hirayama, Y., Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). [link to RRC of NBRP]
Test sheet RDB15973_A9Ijp1-2.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは，非営利の学術研究に限って提供する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15973 pBS423 delta repA DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pBS423 delta repA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15973).

Reference section:

Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. [link to RRC of NBRP]
reference O'Connell Motherway, M., Carbohydrate Syntrophy enhances the establishment of Bifidobacterium breve UCC2003 in the neonatal gut. Sci. Rep. 8 (1): 10627 (2018). PMID 30006512.
reference Nishiyama, K., Bifidobacterium bifidum Extracellular Sialidase Enhances Adhesion to the Mucosal Surface and Supports Carbohydrate Assimilation. MBio 8 (5). pii: e00928-17 (2017). PMID 28974612.
reference Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016).